Static mixing core for preparing LNP liquid preparation
The three-stage flow channel structure combining linear and annular flow channels solves the problem of unsatisfactory mixing effect in traditional microchannels, and realizes uniform mixing and mass production of LNP.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-06
- Publication Date
- 2026-03-17
AI Technical Summary
Traditional T-type and Y-type microchannels do not provide ideal mixing results and make it difficult to achieve uniform mixing of LNPs.
A three-stage flow channel structure combining linear and annular flow channels is adopted. By setting long and short flow channels, a velocity difference is generated to promote the collision of the mixture. Combined with internal threaded connection, the mixing effect is improved.
It improves the uniformity of liposome nanoparticles, supports mass production, and facilitates single-use.
Smart Images
Figure CN223995835U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of liquid mixer technology, specifically to a static mixing core for LNP liquid preparation. Background Technology
[0002] Nanomaterials have gained widespread recognition for their therapeutic potential as novel drug delivery systems that complement traditional pharmacology. They can effectively deliver cytotoxic chemotherapy drugs, antibiotics, and nucleic acid therapy drugs. Liposome nanoparticles (LNPs) have attracted great attention in preclinical and clinical studies due to their significant pharmacological properties, such as protecting drugs from degradation, improving solubility, regulating drug release, and altering drug biodistribution, as well as their excellent therapeutic effects, such as enabling targeted drug delivery to disease sites.
[0003] Microfluidic processes are a highly successful technology for the industrial production of various liquid nanoparticles (LNPs). Compared to traditional mixing methods, the small size of microfluidic channels offers advantages in heat and mass transfer. Rapid mixing at the microscale using microfluidic devices enables more uniform and repeatable nanoparticle formation and can be used for mass production. By controlling parameters such as flow rate, concentration, and mixing ratio, the size, drug loading capacity, and stability of LNPs can be specifically customized.
[0004] T-type and Y-type microchannels are the earliest and simplest microchips for producing liquid nuclei (LNPs). After introducing lipids and a buffer solution at the inlet, the LNP forms at the liquid interface through dilution with an organic solvent based on molecular diffusion. However, the mixing effect of traditional T-type and Y-type channels is not ideal. Summary of the Invention
[0005] To address the above technical problems, the purpose of this invention is to provide a static mixing core for LNP solution preparation with good mixing effect.
[0006] The technical solution of this utility model is: a static mixing core for LNP solution preparation, comprising a box body and a mixing body, wherein the mixing body is disposed inside the box body, and the mixing body includes an inlet, an outlet, and a flow channel. The openings of the inlet and outlet are disposed on the upper surface of the box body, and the flow channel connects the inlet and the outlet.
[0007] In a preferred embodiment, there are two liquid inlets, namely a first liquid inlet and a second liquid inlet; the flow channel includes a primary flow channel, a secondary flow channel and a tertiary flow channel, one end of the primary flow channel is connected to the first liquid inlet and the second liquid inlet, and the other end is connected to the secondary flow channel, and one end of the tertiary flow channel is connected to the secondary flow channel, and the other end is connected to the liquid outlet.
[0008] In a further technical solution, the primary flow channel includes a first inlet flow channel connected to the first inlet port, a second inlet flow channel connected to the second inlet port, and a mixing flow channel connecting the first inlet flow channel and the second inlet flow channel.
[0009] In the above technical solution, the liquid in the first liquid inlet channel and the liquid in the second liquid inlet channel are initially mixed in the mixing channel.
[0010] In a further technical solution, the first liquid inlet channel, the second liquid inlet channel, and the mixing channel are all linear channels, the length of the first liquid inlet channel is less than the length of the second liquid inlet channel, and the vertical cross-sectional shapes of the first liquid inlet channel, the second liquid inlet channel, and the mixing channel are the same.
[0011] In a further technical solution, the secondary flow channel is formed by connecting multiple annular flow channels.
[0012] In a further technical solution, the annular flow channel includes an upper connecting port and a lower connecting port, the upper connecting port and the lower connecting port are located on the same semicircle, and the upper connecting port and the lower connecting port divide the annular flow channel into a long flow channel and a short flow channel.
[0013] In the above technical solution, there are 3-10 annular flow channels, and two adjacent annular flow channels are connected through an upper or lower connecting port.
[0014] In a further technical solution, the three-stage flow channel is a linear flow channel, and the vertical cross-sectional shape of the three-stage flow channel is the same as that of the first liquid inlet flow channel.
[0015] In a preferred embodiment, the inlet and outlet are cylindrical, and the inner surfaces of the inlet and outlet are provided with internal threads.
[0016] In the above technical solution, the internal thread design allows for a more airtight connection between the external connector and the inlet and outlet, resulting in better injection and mixing effects.
[0017] The advantages of this utility model are:
[0018] 1. This utility model adopts a three-stage flow channel combination of linear flow channel and annular flow channel, which can effectively improve the uniformity of liposome nanoparticles;
[0019] 2. The arrangement of long and short channels within the annular flow channel of this utility model can create a velocity difference after the mixed liquid enters. When the two mixed liquids with velocity differences collide, the uniformity of the liposome nanoparticles can be improved.
[0020] 3. This utility model supports single-use and is convenient for mass production. Attached Figure Description
[0021] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the following description of the embodiments will be briefly introduced. The drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments:
[0023] Figure 1 This is a perspective view of the present utility model;
[0024] Figure 2 This is a top view of the present invention;
[0025] Figure 3 This is a schematic diagram of the internal structure of this utility model;
[0026] Figure 4 This is a schematic diagram of the hybrid structure of this utility model;
[0027] Figure 5 This is a schematic diagram of the flow channel structure of this utility model;
[0028] Figure 6 This is a top view of the flow channel of this utility model;
[0029] Figure 7 for Figure 4 Enlarged view of point A in the middle;
[0030] Figure 8 for Figure 6 Enlarged view of section B in the middle.
[0031] Among them: 1. First liquid inlet; 2. Second liquid inlet; 3. Liquid outlet; 4. First liquid inlet channel; 5. Second liquid inlet channel; 6. Mixing channel; 7. Annular channel; 8. Tertiary channel; 9. Upper connecting port; 10. Lower connecting port; 11. Long channel; 12. Short channel. Detailed Implementation
[0032] Example: Figure 1-3 As shown in Figure 7, a static mixing core for LNP solution preparation includes a box and a mixing body. The mixing body is disposed inside the box and includes an inlet, an outlet 3, and a flow channel. The openings of the inlet and outlet 3 are disposed on the upper surface of the box, and the flow channel connects the inlet and outlet 3.
[0033] like Figure 4As shown, there are two inlets, namely the first inlet 1 and the second inlet 2; the flow channel includes a primary flow channel, a secondary flow channel and a tertiary flow channel 8. One end of the primary flow channel is connected to the first inlet 1 and the second inlet 2, and the other end is connected to the secondary flow channel. One end of the tertiary flow channel 8 is connected to the secondary flow channel, and the other end is connected to the outlet 3.
[0034] The flow channel diameter is 0.3 mm. The suitable flow rate range is 3-25 ml / min.
[0035] like Figure 5 , 6 As shown, the primary flow channel includes a first inlet flow channel 4 connected to the first inlet port 1, a second inlet flow channel 5 connected to the second inlet port 2, and a mixing flow channel 6 connecting the first inlet flow channel 4 and the second inlet flow channel 5.
[0036] The first inlet channel 4, the second inlet channel 5, and the mixing channel 6 are all linear channels. The length of the first inlet channel 4 is less than the length of the second inlet channel 5. The vertical cross-sectional shapes of the first inlet channel 4, the second inlet channel 5, and the mixing channel 6 are the same, and the vertical cross-section is rectangular.
[0037] The secondary flow channel is composed of multiple annular flow channels 7 connected together.
[0038] The annular flow channel 7 includes an upper connecting port 9 and a lower connecting port 10. The upper connecting port 9 and the lower connecting port 10 are located on the same semicircle. The upper connecting port 9 and the lower connecting port 10 divide the annular flow channel 7 into a long flow channel 11 and a short flow channel 12.
[0039] There are 7 annular flow channels 7, and two adjacent annular flow channels 7 are connected by an upper connecting port 9 or a lower connecting port 10.
[0040] The third-stage flow channel 8 is a linear flow channel, and the vertical cross-sectional shape of the third-stage flow channel 8 is the same as that of the first liquid inlet flow channel 4.
[0041] The inlet and outlet 3 are cylindrical, and the inner surfaces of the inlet and outlet 3 are provided with internal threads.
[0042] The internal thread design allows for a tighter connection between the external connector and the inlet and outlet 3, resulting in better injection and mixing.
[0043] How to use this embodiment:
[0044] A lipid solution connector is installed at the first inlet 1, and an acetic acid solution connector is installed at the second inlet 2. The lipid solution enters the first inlet channel 4, and the acetic acid solution enters the second inlet channel 5. The two solutions initially come into contact and mix in the mixing channel 6. The mixed solution enters the first annular channel 7, where it is split into two streams: one through a short channel 12, and the other through a long channel 11. Due to the arrangement of the long and short channels 12, the pressure and flow rate of the mixed solution in the long and short channels 11 are different. After colliding and mixing through the long and short channels 11 and 12, the two streams of mixed solution enter the second annular channel 7, continuing to split and collide until they collide and mix again at the lower connecting port 10 of the seventh annular channel 7. The mixed solution then enters the tertiary channel 8 and exits from the outlet 3 to the external equipment.
Claims
1. A static mixing core for LNP solution preparation, comprising a box body, a mixing body, the mixing body is arranged in the box body, characterized in that: The mixing body comprises a liquid inlet, a liquid outlet and a flow channel, the liquid inlet and the liquid outlet are arranged on the upper surface of the box body, and the flow channel is communicated with the liquid inlet and the liquid outlet.
2. The static mixing core for LNP liquid preparation according to claim 1, characterized in that: The liquid inlet is provided with two first liquid inlets and second liquid inlets; the flow channel comprises a first flow channel, a second flow channel and a third flow channel, one end of the first flow channel is communicated with the first liquid inlet and the second liquid inlet, the other end is communicated with the second flow channel, one end of the third flow channel is communicated with the second flow channel, and the other end is communicated with the liquid outlet.
3. The static mixing core for LNP liquid preparation according to claim 2, characterized in that: The first flow channel comprises a first liquid inlet flow channel communicated with the first liquid inlet, a second liquid inlet flow channel communicated with the second liquid inlet, and a mixing flow channel communicated with the first liquid inlet flow channel and the second liquid inlet flow channel.
4. The static mixing core for LNP liquid preparation according to claim 3, characterized in that: The first liquid inlet flow channel, the second liquid inlet flow channel and the mixing flow channel are linear flow channels, the length of the first liquid inlet flow channel is less than the length of the second liquid inlet flow channel; the vertical cross-sectional shape of the first liquid inlet flow channel, the second liquid inlet flow channel and the mixing flow channel is the same.
5. The static mixing core for LNP liquid preparation according to claim 2, characterized in that: The second flow channel is communicated by a plurality of annular flow channels.
6. A static mixing core for LNP solution preparation according to claim 5, characterized in that: The annular flow channel comprises an upper communication port and a lower communication port, the upper communication port and the lower communication port are located on the same semicircle, and the upper communication port and the lower communication port divide the annular flow channel into a long flow channel and a short flow channel.
7. The static mixing core for LNP liquid preparation according to claim 3, characterized in that: The third flow channel is a linear flow channel, and the vertical cross-sectional shape of the third flow channel is the same as that of the first liquid inlet flow channel.
8. The static mixing core for LNP liquid preparation according to claim 1, characterized in that: The liquid inlet and the liquid outlet are cylindrical, and the inner surface of the liquid inlet and the liquid outlet is provided with internal threads.