Rapid protein membrane transfer device

Abstract

The utility model relates to the technical field of protein membrane transfer equipment, in particular to a rapid protein membrane transfer device which comprises a membrane transfer box, a carrier plate and a membrane transfer box, the carrier plate is fixedly connected with the inner wall of the membrane transfer box, a placing groove is formed in the upper surface of the carrier plate, the membrane transfer box is arranged in the placing groove, and a plurality of supporting legs are fixedly connected to the upper surface of the membrane transfer box. Compared with the prior art, by arranging the membrane transfer box, the plug-in assembly and the limiting assembly, the membrane transfer box is electrically connected through the plug-in assembly, so that a plug is inserted into a socket and then is connected with an external power supply connector, and at the moment, membrane transfer can be carried out on rapid protein; according to the technical scheme, the power supply connector is fixed through the fixing assembly, then the external power supply connector is fixed through the limiting assembly, falling of the power supply connector is effectively avoided, damage to a socket is avoided, the rotating film boxes are quite convenient and rapid to mount and dismount, the three rotating film boxes can be operated at the same time, and the operation efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of protein transfer equipment technology, specifically a rapid protein transfer device. Background Technology

[0002] The principle of protein transfer is to use an electric field to transfer proteins from a gel to a solid support (such as an NC membrane or a PVDF membrane). During the transfer process, the electric field is perpendicular to the gel and the membrane, causing the negatively charged proteins to be released under the influence of the current. Through interaction with the transfer membrane, they are firmly adsorbed and fixed on the membrane, maintaining their relative position in the gel.

[0003] The prior art publication number CN209513819U discloses a rapid protein transfer device, comprising a transfer chamber with an electrode interface at one end. Inside the transfer chamber is a carrier plate fixed within the chamber, and the carrier plate has a fitting groove into which a transfer box is fitted. An electrode tip is located on the inner wall of one end of the fitting groove, and this electrode tip is connected to the electrode interface via an electrode wire. One end of the transfer box has an electrode connector, and electrode plates are located inside the transfer box, each fixed to the internal interface of the electrode connector. This design allows for simple transfer; during transfer, the transfer box containing the material is simply inserted into the fitting groove of the carrier plate, and the electrode connector on the transfer box engages with the electrode tip on the groove wall. Electrodes can then be directly connected via the electrode interface. This method simplifies the cumbersome process of manually connecting electrodes and shortens the transfer time.

[0004] With the above setup, the existing protein transfer device, by setting up a transfer box and a transfer container, allows the gel and membrane to connect with the electrode plates inside the transfer container when the material is first loaded into the transfer container in a specific order. During transfer, the transfer container containing the material is simply inserted into the fitting groove of the carrier plate, and the electrode connector on the transfer container connects with the electrode tip on the groove wall, allowing direct electrode connection via the electrode interface. However, the existing protein transfer device has the following drawbacks during operation:

[0005] When using the protein transfer device described in the prior art, the transfer box containing the material needs to be embedded into the fitting groove first, and then an external power supply is used to electrically connect to the electrode connector through the electrode interface. However, due to the mismatch between the size of the electrode interface and the electrode connector, it is quite troublesome to connect the external power supply. It requires manual connection by the operator, which is very cumbersome. Moreover, the external power supply is prone to detachment from the electrode connector after being hit, which can easily damage the electrode connector. Utility Model Content

[0006] The present invention aims to provide a rapid protein transfer device, which mainly solves the technical problem that the external power supply is cumbersome to connect to the electrode connector through the electrode interface in the existing technology, and is also prone to detachment from the external power supply and electrode connector, causing damage to the electrode connector.

[0007] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0008] A rapid protein transfer device includes a transfer chamber, a carrier plate, and a transfer box. The carrier plate is fixed to the inner wall of the transfer chamber, and a placement groove is formed on the upper surface of the carrier plate. The transfer box is located inside the placement groove, and multiple support legs are fixedly connected to the upper surface of the transfer box. The bottom surface of the transfer box abuts against the top surface of the support legs. An insertion port is provided on one side of the transfer box, and a movable groove is formed on the inner wall of one side of the transfer chamber. A plug-in component is installed inside the movable groove.

[0009] The working principle and beneficial effects of this utility model:

[0010] 1. Working principle: When rapid protein transfer is required, first pull the slide bar. The slide bar moves the L-shaped plate and compresses the spring. Then, one of the transfer boxes is placed into the placement slot, and the other transfer boxes are stacked inside the transfer chamber. Then, release the slide bar. Under the action of the spring return force, the L-shaped plate moves the plug into the socket. Then, connect the power connector to the conductive terminal and adjust the limiting component. At this time, rapid protein transfer can be performed.

[0011] 2. Beneficial effects:

[0012] Existing technologies, through the use of a transfer chamber and transfer box, allow for direct connection of the gel and membrane to the electrode plates within the transfer box when materials are loaded in a specific order. During transfer, the transfer box containing the material is simply inserted into the fitting slot of the carrier plate, and the electrode connectors on the transfer box connect to the electrode terminals on the groove wall. Electrode connection is achieved directly through the electrode interface. However, existing protein transfer devices require the material-loaded transfer box to be inserted into the fitting slot first, followed by an external power supply connected to the electrode connectors via the electrode interface. This connection is cumbersome due to the size mismatch between the electrode interface and the electrode connectors, necessitating manual intervention. The previous manual connection process was cumbersome, and the external power supply was prone to detachment from the electrode connector after impact, potentially damaging the connector. This solution addresses this issue by using a transfer box, a plug-in assembly, and a limiting assembly. The plug-in assembly electrically connects the transfer box, allowing the plug to be inserted into the socket. Then, the external power connector is connected, enabling rapid protein transfer. The limiting assembly secures the external power connector, effectively preventing it from detaching and damaging the socket. The installation and removal of the transfer box are quick and easy, and three transfer boxes can be operated simultaneously, significantly improving operational efficiency.

[0013] Preferably, the plug-in assembly includes an L-shaped plate with a fixed cavity inside. The L-shaped plate is slidably connected to a movable groove. A compression spring is fixedly connected to the inner wall of the movable groove, and the other end of the compression spring is fixedly connected to the L-shaped plate. A slide rod is fixedly connected to one side of the L-shaped plate, passing through the transfer chamber and slidably connected thereto. A conductive plate is fixedly connected to the inner wall of the fixed cavity. Multiple plugs are fixedly connected to one side of the conductive plate, passing through the L-shaped plate and fixedly connected thereto. The plugs are inserted into corresponding sockets. A conductive terminal is fixedly connected to one end of the conductive plate, and a power connector is inserted into the outer wall of the conductive terminal. A limiting assembly for fixing the power connector is fixedly connected to the outer wall of the transfer chamber. By setting the plug-in assembly, the plugs and sockets can be tightly connected. Then, by inserting the power connector into the conductive terminal, power can be supplied to the transfer chamber, thereby realizing the rapid transfer of proteins.

[0014] Preferably, the limiting component includes a fixed block, a movable groove on one side of the fixed block, a limiting block slidably connected to the inner wall of the movable groove, symmetrical pull rods fixedly connected to the end of the limiting block away from the power connector, the pull rods passing through the fixed block and slidably connected to it, a limiting spring fixedly connected to the outer wall of the pull rods, one end of the limiting spring fixedly connected to the limiting block, the other end of the limiting spring fixedly connected to the movable groove, a pull plate fixedly connected to the end of the pull rod away from the limiting block, and a limiting groove on one side of the power connector, with the limiting block inserted into the limiting groove. By setting the limiting component, the power connector can be fixed, preventing the power connector from detaching from the conductive terminals when pulled, thus avoiding damage to the conductive terminals.

[0015] Preferably, the upper surface of the transfer box is symmetrically provided with sliding grooves, and the outer wall of the transfer box is fixedly connected with symmetrical sliders, which are slidably connected to the sliding grooves. By setting the sliding grooves and sliders, the transfer box can be limited to prevent it from moving and causing the plug to be unable to be inserted into the socket.

[0016] Preferably, the inner wall of the movable groove is symmetrically provided with guide grooves, and a guide block is slidably connected to the inner wall of the guide groove. The guide block is fixedly connected to the L-shaped plate. By providing guide grooves and guide blocks, the L-shaped plate can be limited, preventing the L-shaped plate from detaching from the movable groove.

[0017] Preferably, the transfer boxes are stacked vertically, and there are three transfer boxes. This improves work efficiency. Attached Figure Description

[0018] Figure 1 This is a structural diagram of the present utility model patent;

[0019] Figure 2 This is a cross-sectional structural diagram of the present utility model patent;

[0020] Figure 3 This utility model patent Figure 2 Structural diagram at point A;

[0021] Figure 4 This is a structural diagram of the internal structure of the transfer box of this utility model patent.

[0022] The reference numerals in the accompanying drawings of the instruction manual include: 1. Transfer box; 2. Carrier plate; 3. Transfer container; 4. Placement slot; 5. Support leg; 6. Socket; 7. Movable slot; 8. L-shaped plate; 9. Fixed cavity; 10. Conductive plate; 11. Plug; 12. Compression spring; 13. Conductive terminal; 14. Power connector; 15. Fixed block; 16. Moving slot; 17. Limiting block; 18. Pull rod; 19. Limiting spring; 20. Pull plate; 21. Limiting slot; 22. Slide groove; 23. Slider; 24. Guide groove; 25. Guide block; 26. Slide rod. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] like Figures 1-4 As shown, a rapid protein transfer device includes a transfer chamber 1, a carrier plate 2, and a transfer container 3. The upper surface of the transfer chamber 1 has symmetrically formed grooves 22. Symmetrical sliders 23 are fixed to the outer wall of the transfer container 3, and the sliders 23 are slidably connected to the grooves 22. The carrier plate 2 is fixed to the inner wall of the transfer chamber 1, and a placement groove 4 is formed on its upper surface. The transfer container 3 is located inside the placement groove 4. Multiple support legs 5 are fixedly connected to the upper surface of the transfer container 3, and the bottom surface of the transfer container 3 abuts against the top surface of the support legs 5. An insertion device is provided on one side of the transfer container 3. A movable groove 7 is formed on one inner wall of the transfer box 1. An insertion assembly is installed inside the movable groove 7. The insertion assembly includes an L-shaped plate 8, which has a fixed cavity 9. The L-shaped plate 8 is slidably connected to the movable groove 7. Guide grooves 24 are symmetrically formed on the inner wall of the movable groove 7. A guide block 25 is slidably connected to the inner wall of the guide groove 24 and is fixedly connected to the L-shaped plate 8. A compression spring 12 is fixedly connected to the inner wall of the movable groove 7, and the other end of the compression spring 12 is fixedly connected to the L-shaped plate 8. A sliding rod 26 is fixedly connected to one side of the L-shaped plate 8. Rod 26 passes through and slides through the transfer chamber 1. A conductive plate 10 is fixedly attached to the inner wall of the fixed cavity 9. Multiple plugs 11 are fixedly attached to one side of the conductive plate 10. The plugs 11 pass through and are fixedly attached to the L-shaped plate 8. The plugs 11 are inserted into the sockets 6. A conductive terminal 13 is fixedly attached to one end of the conductive plate 10. A power connector 14 is inserted into the outer wall of the conductive terminal 13. A limiting assembly for fixing the power connector 14 is fixedly attached to the outer wall of the transfer chamber 1. The limiting assembly includes a fixing block 15. A moving groove 16 is formed on one side of the fixing block 15. The inner wall of the moving groove 16 is slidably connected to a limiting block 17. The end of the limiting block 17 away from the power connector 14 is fixedly connected to a symmetrical pull rod 18. The pull rod 18 passes through the fixing block 15 and is slidably connected to it. The outer wall of the pull rod 18 is fixedly connected to a limiting spring 19. One end of the limiting spring 19 is fixedly connected to the limiting block 17, and the other end of the limiting spring 19 is fixedly connected to the moving groove 16. The end of the pull rod 18 away from the limiting block 17 is fixedly connected to a pull plate 20. A limiting groove 21 is opened on one side of the power connector 14, and the limiting block 17 is inserted into the limiting groove 21.

[0025] As can be seen from the above, the specific embodiments of this utility model are as follows:

[0026] When rapid protein transfer is required, first pull the slide bar 26. The slide bar 26 moves the L-shaped plate 8 and compresses the compression spring 12. Then, one of the transfer boxes 3 is placed into the placement slot 4, and the other transfer boxes 3 are stacked inside the transfer box 1. Then, release the slide bar 26. Under the action of the spring force of the compression spring 12, the L-shaped plate 8 moves the plug 11 into the socket 6. Then, pull the pull plate 20. The pull plate 20 moves the pull rod 18. The pull rod 18 moves the limiting block 17, so that the limiting block 17 compresses the limiting spring 19. Then, the power connector 14 is plugged into the conductive terminal 13. Then, release the pull plate 20. Under the action of the limiting spring 19, the limiting block 17 is locked into the limiting slot 21. At this time, rapid protein transfer can be performed.

[0027] The above descriptions are merely embodiments of this utility model, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A rapid protein transfer blotting device comprising a blotting tank (1), a carrier plate (2) and a blotting cassette (3), characterized in that, The carrier plate (2) is fixed on the inner wall of the membrane transfer box (1), the upper surface of the carrier plate (2) is provided with a placing groove (4), the membrane transfer box (3) is arranged in the placing groove (4), the upper surface of the membrane transfer box (3) is fixedly connected with a plurality of supporting legs (5), the bottom surface of the membrane transfer box (3) is in abutment with the top surface of the supporting leg (5), one side of the membrane transfer box (3) is provided with a socket (6), and one side of the inner wall of the membrane transfer box (1) is provided with a movable groove (7), and the movable groove (7) is internally provided with a plug-in assembly.

2. The rapid protein transfer blotting apparatus of claim 1, wherein: The plug-in assembly comprises an L-shaped plate (8), a fixed cavity (9) is formed in the L-shaped plate (8), the L-shaped plate (8) is slidably connected with the movable groove (7), a compression spring (12) is fixedly connected to the inner wall of the movable groove (7), the other end of the compression spring (12) is fixedly connected with the L-shaped plate (8), one side of the L-shaped plate (8) is fixedly connected with a sliding rod (26), the sliding rod (26) penetrates through the membrane transfer box (1) and is slidably connected with the membrane transfer box (1), the inner wall of the fixed cavity (9) is fixedly connected with a conductive plate (10), a plurality of plugs (11) are fixedly connected to one side of the conductive plate (10), the plugs (11) penetrate through the L-shaped plate (8) and are fixedly connected with the L-shaped plate (8), the plugs (11) are correspondingly plugged into the socket (6), one end of the conductive plate (10) is fixedly connected with a conductive terminal (13), the outer wall of the conductive terminal (13) is plugged into a power connector (14), and the outer wall of the membrane transfer box (1) is fixedly connected with a limiting assembly for fixing the power connector (14).

3. The rapid protein transfer blotting apparatus of claim 2, wherein: The limiting assembly comprises a fixed block (15), a moving groove (16) is formed in one side of the fixed block (15), a limiting block (17) is slidably connected to the inner wall of the moving groove (16), symmetrically arranged pull rods (18) are fixedly connected to the end of the limiting block (17) away from the power connector (14), the pull rods (18) penetrate through the fixed block (15) and are slidably connected with the fixed block (15), a limiting spring (19) is fixedly connected to the outer wall of the pull rod (18), one end of the limiting spring (19) is fixedly connected with the limiting block (17), the other end of the limiting spring (19) is fixedly connected with the moving groove (16), a pull plate (20) is fixedly connected to the end of the pull rod (18) away from the limiting block (17), a limiting groove (21) is formed in one side of the power connector (14), and the limiting block (17) is plugged into the limiting groove (21).

4. The rapid protein transfer blotting apparatus of claim 1, wherein: The upper surface of the membrane transfer box (1) is symmetrically provided with a sliding groove (22), and the outer wall of the membrane transfer box (3) is fixedly connected with symmetrically arranged sliding blocks (23), the sliding blocks (23) are slidably connected with the sliding groove (22).

5. The rapid protein transfer blotting apparatus of claim 1, wherein: The inner wall of the movable groove (7) is symmetrically provided with a guide groove (24), and the inner wall of the guide groove (24) is slidably connected with a guide block (25), and the guide block (25) is fixedly connected with the L-shaped plate (8).

6. The rapid protein transfer blotting apparatus of claim 1, wherein: The membrane transfer boxes (3) are arranged in a stacked manner, and three membrane transfer boxes (3) are arranged.

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