High-throughput semi-automatic oocyte and embryo freezing carrier
By designing a high-throughput semi-automated oocyte and embryo cryopreservation carrier, the problems of limited quantity and waste of consumables in a single cryopreservation were solved, realizing efficient cryopreservation of multiple cells/embryos, and improving the cryopreservation effect and ease of operation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-17
- Publication Date
- 2026-03-20
AI Technical Summary
Existing cryogenic carriers have a limited number of cells that can be frozen at one time, the freezing effect is greatly affected by the operator's skill level, and multiple transfers of cells/embryos are required, resulting in waste of consumables.
Design a high-throughput semi-automated oocyte and embryo cryopreservation carrier, comprising blades and a handle, and equipped with a cryopreservation operation area, a sample placement area, and a reagent outflow area. The channel has sample loading holes to precisely control the volume of cryoprotectant, enabling single-cycle multi-cell/embryo cryopreservation.
It increases freezing throughput, reduces operational difficulty, decreases consumable usage, simplifies the operation process, and improves the stability and efficiency of freezing effects.
Smart Images
Figure CN224007630U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of ultralow temperature frozen preservation, in particular to a high-throughput semi-automatic oocyte and embryo freezing carrier. BACKGROUND
[0002] The freezing carrier is a bearing device for oocyte / embryo freezing preservation, and the commonly used carriers in the freezing at present mainly include the thin tube method, the open type elongated plastic thin tube method, the solid surface method, the ultrafine open type elongated plastic thin tube method, the electron microscope copper mesh method, the Cryotop method, the drop freezing method and the freezing ring method etc. Researches point out that the Cryotop method is the most commonly used and effective freezing carrier, and can significantly improve the cooling rate through the minimum freezing protective agent loading volume, and is more beneficial to the oocyte / embryo to form the vitrification state and reduce the freezing damage.
[0003] The traditional Cryotop freezing carrier is composed of a blade and a handle, and has a simple structure. The volume of the freezing protective liquid dropped on the carrier directly affects the cooling speed during the freezing operation, and has a decisive influence on the freezing effect of the oocyte / embryo. In order to guarantee the best freezing efficiency, the existing Cryotop freezing carrier usually freezes at most about 5 oocytes / embryos at a time, which reduces the freezing throughput and the effective utilization rate of the carrier. Moreover, the volume of the dropped freezing protective liquid is directly related to the technical proficiency of the operator, which leads to a large difference in the freezing effect of different operators. In addition, when the existing freezing carrier is used for freezing operation, the oocyte / embryo needs to be balanced in an additional operation dish for the freezing balancing liquid and the vitrification freezing liquid, and there is a phenomenon of multiple transfers of the oocyte / embryo and waste of reagents and consumables. Therefore, the technical personnel in the field provide a high-throughput semi-automatic oocyte and embryo freezing carrier to solve the problems in the above background technology. SUMMARY
[0004] (I) Technical problems solved
[0005] In view of the deficiencies of the prior art, the utility model provides a high-throughput semi-automatic oocyte and embryo freezing carrier to solve the problems that the volume of the freezing protective liquid dropped by different operators is different, which leads to a large difference in the freezing effect, and when the existing freezing carrier is used for freezing operation, the oocyte / embryo needs to be balanced in an additional operation dish for the freezing balancing liquid and the vitrification freezing liquid, and there is a phenomenon of multiple transfers of the oocyte / embryo and waste of reagents and consumables.
[0006] (II) Technical solutions
[0007] To achieve the above object, the utility model provides the following technical scheme: A high flux semi -automatic oocyte and embryo freezing carrier, including blade and handle, the blade and handle are connected, the blade is equipped with freezing operation area and bearing area, the freezing operation area includes reagent inflow area, sample placement area and reagent outflow area, the sample placement area includes the flue, the flue is equipped with sample addition hole, one end of the flue is communicated with reagent inflow area, and the other end of the flue is communicated with reagent outflow area.
[0008] Preferably, the flue is provided with three, the flue is parallel with each other, one end of each flue is communicated with reagent inflow area, and the other end is communicated with reagent outflow area, when the blade is placed horizontally, the freezing protectant flows from reagent inflow area to reagent outflow area through the flue, after the blade is inclined, the liquid in reagent outflow area can be discharged.
[0009] Preferably, the number of sample addition holes on each flue is four, the four sample addition holes are arranged in parallel, and the four sample addition holes are communicated with each other in pairs, the freezing protectant can flow from the sample addition hole adjacent to reagent inflow area to the subsequent sample addition hole in turn until reagent outflow area.
[0010] Preferably, the inner diameter of the sample addition hole is 0.4mm, and the height is 0.1mm.
[0011] (Three) beneficial effects
[0012] Compared with the prior art, the utility model provides a high flux semi -automatic oocyte and embryo freezing carrier, which has the following beneficial effects:
[0013] Through the design, by setting the inner diameter and height of the sample addition hole, the volume of the freezing protectant during the freezing of the oocyte / embryo is accurately controlled, which not only ensures the rapid cooling speed, but also reduces the technical difficulty of the freezing operation, significantly improves the freezing effect, and through the setting of three flues and multiple sample addition holes, the number of oocyte / embryo frozen at a time can be significantly increased, which helps to improve the freezing flux. In addition, by setting the freezing operation area on the blade, the entire operation process can be completed on the carrier through the single transfer of the oocyte / embryo, which can simplify the freezing operation process and reduce the use of consumables, and the practicability, effectiveness and operability are significantly improved. BRIEF DESCRIPTION OF DRAWINGS
[0014] Figure 1 It is a kind of high flux semi -automatic oocyte and embryo freezing carrier's stereoscopic structure schematic diagram provided by the embodiment of the present application.
[0015] Figure 2 It is the structure enlarged view of freezing operation area in the high flux semi -automatic oocyte and embryo freezing carrier provided by the embodiment of the present application.
[0016] Figure 3 is a side view of a high-throughput semi-automatic oocyte and embryo freezing carrier sample adding hole provided by the embodiment of the application.
[0017] In the figure: 1, blade; 2, handle; 3, freezing operation area; 4, bearing area; 5, sample inflow area; 6, sample placement area; 7, reagent outflow area; 8, channel; 9, sample adding hole. DETAILED DESCRIPTION
[0018] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application.
[0019] The application provides a technical scheme, a high-throughput semi-automatic oocyte and embryo freezing carrier, please refer to Figure 1 、 Figure 2 、 Figure 3 , including blade 1 and handle 2, the blade 1 and handle 2 are connected, the blade 1 is provided with freezing operation area 3 and bearing area 4, the freezing operation area 3 is provided with reagent inflow area 5, sample placement area 6 and reagent outflow area 7, the sample placement area 6 is provided with channel 8, the channel 8 is provided with sample adding hole 9, the inner diameter of the sample adding hole 9 is 0.4mm, the height is 0.1mm, one end of the channel 8 is communicated with the reagent inflow area 5, the other end of the channel 8 is communicated with the reagent outflow area 7.
[0020] Please refer to Figure 1 、 Figure 2 、 Figure 3 , the channel 8 is provided with three, the channels 8 are parallel to each other, one end of each channel 8 is communicated with the reagent inflow area 5, the other end is communicated with the reagent outflow area 7, when the blade 1 is horizontally placed, the freezing protective agent flows from the reagent inflow area 5 to the reagent outflow area 7 through the channel 8, after the blade 1 is inclined, the liquid in the reagent outflow area 7 can be discharged, the number of sample adding holes 9 provided on each channel 8 is four, the four sample adding holes 9 are arranged in parallel, and the four sample adding holes 9 are communicated with each other in pairs, the freezing protective agent can flow from the sample adding hole 9 adjacent to the reagent inflow area 5 to the subsequent sample adding hole 9 in turn until the reagent outflow area 7.
[0021] The high-throughput semi-automatic oocyte and embryo freezing carrier in the application is composed of blade 1 and handle 2, the freezing operation area 3 and the bearing area 4 are respectively arranged on the blade 1, the sample inflow area 5, the sample placement area 6 and the reagent outflow area 7 are arranged in the freezing operation area 33.
[0022] The sample placement area 6 is provided with three independent parallel channels 8, one end of each channel 8 is communicated with the sample inflow area 5, and the other end is communicated with the reagent outflow area 7, the cryoprotective agent passes through the sample inflow area 5 and the channel 8 and then reaches the reagent outflow area 7, and four circular sample addition holes 9 are embedded in each independent channel 8, each circular sample addition hole 9 is 0.4mm in diameter and 0.1mm in height, the circular sample addition holes 9 are arranged in parallel and communicated with each other;
[0023] The fixed-volume circular sample addition hole 9 arranged on the blade 1 can accurately control the volume of the cryoprotective liquid, realize rapid cooling, and reduce the technical level required for the operator, thereby ensuring the effectiveness and popularity of the freezing operation; the multiple sample addition holes 9 can complete the freezing operation of 12 oocytes / embryos at a time, thereby greatly improving the throughput of the freezing operation; the freezing operation area 3 can realize the freezing operation by single transfer of oocytes / embryos, and does not require other additional consumables, thereby effectively simplifying the freezing operation steps, reducing the freezing cost, and having significant practicability and operability.
[0024] It should be noted that, in the present text, relational terms such as first and second and the like are used merely to differentiate one entity or action from another, without necessarily requiring or implying any actual such relationship or order between or among the entities or actions. Also, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.
[0025] In the present text, unless otherwise explicitly specified and limited, the terms "mount", "set", "connect", "fix", "screw", and the like should be understood in a broad sense, for example, can be fixedly connected, or can be detachably connected, or can be integrated; can be mechanically connected, or can be electrically connected; can be directly connected, or can be indirectly connected through an intermediate medium; can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited, the specific meaning of the above terms in the present utility model can be understood according to the specific circumstances by those skilled in the art.
[0026] Although the embodiments of the present utility model have been shown and described, those skilled in the art can understand that various changes, modifications, replacements, and variations can be made to these embodiments without departing from the principles and spirits of the present utility model, the scope of the present utility model is defined by the appended claims and their equivalents.
Claims
1. A high-throughput semi-automated oocyte and embryo cryopreservation carrier, comprising blades (1) and handles (2), said blades (1) and handles (2) being connected, characterized in that: The blade (1) is provided with a freezing operation area (3) and a carrying area (4). The freezing operation area (3) includes a reagent inflow area (5), a sample placement area (6) and a reagent outflow area (7). The sample placement area (6) includes a channel (8). The channel (8) is provided with a sample addition hole (9). One end of the channel (8) is connected to the reagent inflow area (5), and the other end of the channel (8) is connected to the reagent outflow area (7).
2. The high-throughput semi-automated oocyte and embryo cryopreservation carrier according to claim 1, characterized in that: There are three passageways (8), which are parallel to each other.
3. The high-throughput semi-automated oocyte and embryo cryopreservation carrier according to claim 1, characterized in that: Each of the aforementioned passageways (8) has four sample loading holes (9), which are arranged in parallel.
4. The high-throughput semi-automated oocyte and embryo cryopreservation carrier according to claim 1, characterized in that: The inner diameter of the sample feeding hole (9) is 0.4 mm and the height is 0.1 mm.