Magnetic cell sorting device
By introducing a coil lifting mechanism into the sorting device, the magnetic field is used to move the nanomagnetic beads within the sorting column, solving the problems of slow speed and incomplete sorting in the existing technology, and achieving a faster cell sorting process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-03-24
AI Technical Summary
Existing magnetic cell sorting devices are slow in column-type sorting and have the risk of incomplete sorting, especially due to the speed bottleneck caused by the unidirectional nature of the cell-magnetic bead binding process.
A coil lifting mechanism is adopted, which generates a magnetic field around the sorting column by means of an energized coil and a magnetic ring. Combined with the action of a servo cylinder and an electric push rod, the coil and magnetic ring move up and down around the sorting column, which enhances the movement of the nano magnetic beads inside the sorting column and increases the probability of cell contact with the magnetic beads.
It accelerates cell sorting speed, improves sorting efficiency and thoroughness, and solves the speed bottleneck and incomplete sorting problems existing in the current technology.
Smart Images
Figure CN224025255U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell sorting equipment technical field, concretely relates to a magnetic cell sorting device. BACKGROUND
[0002] The immunomagnetic cell sorting is based on the characteristics that the cell surface antigen can be combined with the specific antibody connected with the magnetic bead, in the external magnetic field, the cell connected with the magnetic bead through the antibody is adsorbed and stays in the magnetic field, and the cell without the surface antigen does not have the magnetism and does not stay in the magnetic field, so as to achieve the purpose of cell separation.
[0003] Among them, the magnetic sorting is also divided into column type and columnless type, the column type sorting needs to use sorting column, sorting support and nanometer magnetic bead, and 20-50nm small magnetic beads are used, the advantage is high purity, but the speed is slow, and the columnless sorting just needs the process from incubation to separation, and the time is basically between 8 to 25 minutes, the columnless sorting does not need the sorting column, and is matched with the medium magnetic bead within 100nm, and the advantage is fast speed. The present inventor finds that in the column type sorting process, the specific antibody combination between the cell to be sorted and the magnetic bead relies on the magnetic attraction of the magnetic bead itself to make the cell close to the magnetic bead, so that the process of the cell close to the magnetic bead is unidirectional, and the magnetic bead is in a relatively static state relative to the cell, so that the speed is slow in the sorting process, and there is the risk of incomplete cell sorting. Therefore, the person skilled in the art provides a magnetic cell sorting device according to the above provided viewpoint, UTILITY MODEL CONTENT
[0004] The utility model discloses a magnetic cell sorting device technical scheme to solve the deficiency in the background art. In order to solve the defects and defects in the background art, the technical scheme has the following contents:
[0005] The coil lifting mechanism is clamped on the left side wall of the coil lifting mechanism.
[0006] The coil lifting mechanism includes a stand, a top plate fixedly connected to the left side top of the stand, and an electric telescopic rod fixed to the upper surface of the top plate, the telescopic shaft of the electric telescopic rod is connected with the telescopic shaft penetrating through the top plate, the bottom end of the telescopic shaft is fixedly connected with a movable block, two servo cylinders are fixed to the left side of the movable block, a double-head electric push rod is connected to the telescopic shaft of the servo cylinder, and clamping plates are fixedly connected to the front and rear telescopic shafts of the double-head electric push rod.
[0007] The power supply coil comprises a sorting column, a magnetic ring on the outer surface of the sorting column, and a coil wound at equal distances on the outer surface of the magnetic ring, and the outer surface of the coil is in clamping contact with the side surface of the clamping plate.
[0008] Specifically, the current supplied to the coil is adjusted by a controller between the coil and the power supply line, the magnetic ring generates a magnetic field by being supplied with power, the cells to be sorted and the nanometer magnetic beads are then put into the sorting column, the two telescopic shafts of the double-head electric push rod drive the clamping plates to approach each other and clamp the coil, the coil and the magnetic ring are coaxially arranged with the sorting column by the extension and retraction of the servo cylinder, the extension and retraction of the electric telescopic rod drive the telescopic shaft to pull the movable block to move up and down, and the clamping plates clamp the magnetic ring and the coil to move up and down on the periphery of the sorting column in the process of the movable block moving up and down, the nanometer magnetic beads in the sorting column are moved by the magnetic field generated by the magnetic ring and the coil, so that the contact probability of the cells and the nanometer magnetic beads is increased and the sorting speed is accelerated.
[0009] As a preferred scheme of the utility model, a plurality of magnetic beads are arranged in the inner cavity of the sorting column, and the cells to be sorted are arranged in the inner cavity of the sorting column.
[0010] As a preferred scheme of the utility model, the sorting column and the magnetic ring are coaxially arranged, and the inner side surfaces of the magnetic ring and the coil are spaced apart from the outer surface of the sorting column by a distance of 1-3 cm.
[0011] As a preferred scheme of the utility model, the current input end of the coil is connected with the power supply line through a wire, and a controller is arranged between the coil and the power supply line.
[0012] As a preferred scheme of the utility model, the guide columns penetrating the movable block are fixedly connected to the bottom of the top plate, and the circular holes for the guide columns to penetrate are formed in the front and rear regions of the movable block.
[0013] As a preferred scheme of the utility model, the vertical plate is locked and connected to the left side surface of the movable block by screws, and the telescopic cylinder body of the servo cylinder is connected to the left side surface of the vertical plate.
[0014] As a preferred scheme of the utility model, the support plate is fixedly connected to the distal end of the telescopic shaft of the servo cylinder, and the right side surface of the cylinder body of the double-head electric push rod is fixedly connected to the left side surface of the support plate.
[0015] As a preferred scheme of the utility model, the silica gel pad is fixedly connected to the side surface of the clamping plate approaching each other by an adhesive, and the outer surface of the coil is in contact with the silica gel pad.
[0016] In the above technical scheme, the utility model provides the technical effects and advantages:
[0017] The scheme sets the energized coil and the magnetic ring on the periphery of the sorting column, uses the coil and the magnetic ring to generate a magnetic field on the periphery of the sorting column, attracts the nanometer magnetic beads in the sorting column to move, cooperates with the coil lifting mechanism to pull the coil and the magnetic ring to reciprocate up and down, and then drives the nanometer magnetic beads in the sorting column to move up and down, so as to accelerate the combination with the cells in the sorting column and improve the speed of cell sorting. BRIEF DESCRIPTION OF DRAWINGS
[0018] In order to more clearly explain the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art according to these drawings.
[0019] Figure 1 The schematic view of the cell sorting acceleration mechanism is shown in the figure.
[0020] Figure 2 The schematic view of the coil lifting mechanism is shown in the figure.
[0021] Figure 3 The schematic view of the energized coil is shown in the figure.
[0022] Explanation of reference signs:
[0023] 1, coil lifting mechanism; 11, stand column; 12, movable block; 13, clamping plate; 14, double-head electric push rod; 15, support plate; 16, servo electric cylinder; 17, vertical plate; 18, telescopic shaft; 19, electric telescopic rod; 110, top plate; 111, guide column; 2, energized coil; 21, sorting column; 22, magnetic ring; 23, coil. DETAILED DESCRIPTION
[0024] In order to more clearly explain and illustrate the technical solutions and implementation modes of the present application, the following introduces several preferred specific embodiments for implementing the technical solutions of the present application.
[0025] The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, and uses. It should be understood that throughout the drawings, the same or like reference numerals can be used for the same or like components and features. The various drawings are schematic illustrations only and are not necessarily drawn to scale. Certain portions of the drawings can be shown exaggerated in size, or drawn using a different perspective, for purposes of explanation. Various publications, patents, and published patent specifications referred to in this document are hereby incorporated by reference in their entirety. The technical solutions of the present application will be described clearly and completely below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application.
[0026] Embodiment, a better technical scheme of a magnetic cell sorting device, comprising the following:
[0027] The coil lifting mechanism 1 is provided with an energized coil 2 clamped on the left side wall of the coil lifting mechanism 1. The coil lifting mechanism 1 comprises a stand 11, a top plate 110 fixedly connected to the left top of the stand 11, and an electric telescopic rod 19 fixed to the upper surface of the top plate 110. The telescopic shaft of the electric telescopic rod 19 is connected with a telescopic shaft 18 penetrating through the top plate 110. The bottom end of the telescopic shaft 18 is fixedly connected with a movable block 12. Two servo cylinders 16 are fixed to the left side of the movable block 12. A double-head electric push rod 14 is connected to the telescopic shaft of the servo cylinder 16. A clamping plate 13 is fixedly connected to the front and rear telescopic shafts of the double-head electric push rod 14.
[0028] The energized coil 2 comprises a sorting column 21, a magnetic ring 22 located on the outer surface of the sorting column 21, and a coil 23 wound at equal distances on the outer surface of the magnetic ring 22. The outer surface of the coil 23 is in clamping contact with the side surface of the clamping plate 13. A plurality of magnetic beads are arranged in the inner cavity of the sorting column 21. Cells to be sorted are arranged in the inner cavity of the sorting column 21. The sorting column 21 and the magnetic ring 22 are coaxially arranged. The inner surface of the magnetic ring 22 and the coil 23 has a spacing of 1-3 cm from the outer surface of the sorting column 21. The current inlet end of the coil 23 is connected with the power cord through a wire, and a controller is arranged between the coil 23 and the power cord.
[0029] The bottom of the top plate 110 is fixedly connected with the guide column 111 penetrating the movable block 12, the inner front and back areas of the movable block 12 are provided with the circular holes for the guide column 111 to penetrate, the left side surface of the movable block 12 is fixedly connected with the vertical plate 17 through the screw locking, the telescopic cylinder body of the servo cylinder 16 is connected with the left side surface of the vertical plate 17, the telescopic shaft of the servo cylinder 16 is fixedly connected with the support plate 15, the right side surface of the cylinder body of the double-head electric push rod 14 is fixedly connected with the left side surface of the support plate 15, the side surface of the mutually close clamping plates 13 is fixedly connected with the layer of silica gel pad through the adhesive, and the outer ring surface of the coil 23 is in contact with the silica gel pad.
[0030] According to the above-mentioned preferred technical scheme, the working process of the technical scheme is described.
[0031] The controller between the coil 23 and the power line is used to adjust the current supplied to the coil 23, the coil 23 is powered to generate a magnetic field of the magnetic ring 22, then the cells to be sorted and the nanometer magnetic beads are put into the sorting column 21, the two telescopic shafts of the double-head electric push rod 14 drive the clamping plates 13 to be close to each other and clamp the coil 23, the coil 23 and the magnetic ring 22 are coaxially arranged with the sorting column 21 by the telescopic action of the servo cylinder 16, then the telescopic shaft 18 is pulled by the telescopic action of the electric telescopic rod 19 to drive the movable block 12 to move up and down, and the clamping plates 13 clamp the magnetic ring 22 and the coil 23 to move up and down on the periphery of the sorting column 21 in the process of the movable block 12 moving up and down, the nanometer magnetic beads in the sorting column 21 are moved by the magnetic field generated by the magnetic ring 22 and the coil 23, so that the contact probability of the cells and the nanometer magnetic beads is increased, and the sorting speed is increased.
[0032] The above only describes some exemplary embodiments of the present application by way of illustration, without doubt, for ordinary skilled in the art, without departing from the spirit and scope of the present application, the described embodiments can be modified in various ways. Therefore, the above drawings and description are illustrative in nature and should not be understood as limiting the scope of protection of the present application.
Claims
1. A magnetic cell sorting device, comprising a coil lifting mechanism (1), characterized in that: An energized coil (2) is clamped on the left side wall of the coil lifting mechanism (1); The coil lifting mechanism (1) includes a column (11), a top plate (110) fixedly connected to the top left side of the column (11), and an electric telescopic rod (19) fixed to the upper surface of the top plate (110). The telescopic shaft of the electric telescopic rod (19) is connected to a telescopic shaft (18) that passes through the top plate (110). A movable block (12) is fixedly connected to the bottom end of the telescopic shaft (18). Two servo electric cylinders (16) are fixed to the left side of the movable block (12). A double-headed electric push rod (14) is connected to the telescopic shaft of the servo electric cylinder (16). A clamping plate (13) is fixedly connected to the front and rear telescopic shafts of the double-headed electric push rod (14). The energized coil (2) includes a sorting column (21), a magnetic ring (22) located on the outer surface of the sorting column (21), and a coil (23) wound at equal intervals on the outer ring of the magnetic ring (22). The outer surface of the coil (23) is in contact with the side surface of the clamping plate (13) that is close to each other.
2. The magnetic cell sorting device according to claim 1, characterized in that: The inner cavity of the sorting column (21) is provided with a number of magnetic beads, and the inner cavity of the sorting column (21) is provided with cells to be sorted.
3. The magnetic cell sorting device according to claim 1, characterized in that: The sorting column (21) and the magnetic ring (22) are arranged coaxially, and there is a gap of 1-3 cm between the inner surface of the magnetic ring (22) and the inner surface of the coil (23) and the outer surface of the sorting column (21).
4. The magnetic cell sorting device according to claim 1, characterized in that: The current input terminal of the coil (23) is connected to the power line through a wire, and a controller is provided between the coil (23) and the power line.
5. A magnetic cell sorting device according to claim 1, characterized in that: The bottom front and rear sides of the top plate (110) are fixedly connected to guide posts (111) that pass through the movable block (12), and the front and rear areas of the movable block (12) are provided with round holes for the guide posts (111) to pass through.
6. The magnetic cell sorting device according to claim 1, characterized in that: A vertical plate (17) is screwed to the left side surface of the movable block (12), and the telescopic cylinder of the servo electric cylinder (16) is connected to the left side surface of the vertical plate (17).
7. A magnetic cell sorting device according to claim 1, characterized in that: The telescopic shaft of the servo electric cylinder (16) is fixedly connected to a support plate (15), and the right side surface of the cylinder body of the double-headed electric push rod (14) is fixedly connected to the left side surface of the support plate (15).
8. A magnetic cell sorting device according to claim 1, characterized in that: A silicone pad is fixedly connected to the side surface of the clamps (13) that are close to each other by an adhesive, and the outer ring surface of the coil (23) is in contact with the silicone pad.