Magnetic cell sorting device with sorting column

Abstract

The utility model discloses a magnetic cell sorting device with a sorting column, which comprises a fixed frame, a magnetic sorting mechanism and a moving mechanism, the magnetic sorting mechanism sequentially comprises the sorting column, a limiting sleeve, a first metal shell, a magnetic component and a second metal shell from inside to outside, and the magnet structure of the magnetic component is a Halbach array. The moving mechanism comprises a first shell and a second shell; the magnetic separation mechanism has a first state and a second state, the magnetic assembly is located in the second metal shell all the time, when the magnetic separation mechanism is in the first state, the magnetic separation mechanism is located between the first shell and the second shell, and the first metal shell is located in the magnetic assembly; when the magnetic separation mechanism is in the second state, the first metal shell is located outside the magnetic assembly. The magnetic cell sorting device with the sorting column can effectively and controllably activate and shield a magnetic field, and ensures that an invalid magnetic field is weakened and the magnetic field intensity of a sorting area is enhanced in a working state, so that the magnetic cell sorting effect is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell magnetic force sorting device technical field, concretely relates to a cell magnetic force sorting device with sorting column. BACKGROUND

[0002] In the field of cell sorting, the magnetic force sorting is generally adopted in the market at present, and the magnetic field environment can directly and fundamentally affect the sorting result. For the magnetic sorting device with column, in order to make the labeled cells stay between the magnetic beads in the sorting column, the magnetic field strength of the initial magnetic field required is strong, and the initial magnetic field uniformly covers the magnetized area, so that the magnetic field line passes between the sorting column bodies, and all the magnetic substances in the sorting column are magnetized.

[0003] In the prior art, the magnetic sorting device with column has two problems: (1) the device mostly adopts the magnetic field formed by single or two magnets, and the magnetic surface usually appears the phenomenon that the surface magnetic strength is high in the middle and low on both sides, resulting in weak magnetic field strength and uneven magnetic field distribution, which can affect the sorting effect and sorting flux; (2) the strength of the invalid magnetic field is too high, and in the cell magnetic sorting process, the introduction of the invalid magnetic field with too high strength can bring strong magnetic risk, thereby causing electromagnetic interference to other electrical components; and the grabbing ability of the magnetic labeled cells is affected, thereby leading to the undesirable experimental result. UTILITY MODEL CONTENT

[0004] Therefore, in order to solve the problems in the prior art, the utility model provides a cell magnetic force sorting device with sorting column, which can enhance the strength of the effective magnetic field, reduce the strength of the invalid magnetic field, improve the uniformity of the effective magnetic field coverage, and effectively improve the cell magnetic sorting effect.

[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0006] The utility model provides a cell magnetic sorting device with sorting column, which is filled with magnetic beads in the sorting column, and comprises a fixed frame, a magnetic sorting mechanism and a moving mechanism, the moving mechanism is installed on the fixed frame, the magnetic sorting mechanism is installed on the moving mechanism, the magnetic sorting mechanism comprises, from inside to outside, a sorting column, a limiting sleeve, a first metal shell, a magnetic assembly and a second metal shell, the magnet structure of the magnetic assembly is a Halbach array, the moving mechanism comprises a first shell and a second shell, and the first shell is located above the second shell, the magnetic sorting mechanism has a first state and a second state, when the magnetic sorting mechanism is in the first state, the magnetic sorting mechanism is located between the first shell and the second shell, the first metal shell is located inside the magnetic assembly, and the magnetic assembly is located inside the second metal shell, when the magnetic sorting mechanism is in the second state, the first metal shell is located outside the magnetic assembly, and the magnetic assembly is located inside the second metal shell, and the first metal shell and the second metal shell both have ultrahigh magnetic permeability.

[0007] By adopting the magnetic assembly with the Halbach array magnet structure, a uniform basic magnetic field can be provided, and the synergistic effect between the first metal shell and the sorting column filled with magnetic beads can form a double magnetic field enhancement effect, by increasing the second metal shell and utilizing the cooperation of the second metal shell with the first metal shell and the moving mechanism, in combination with the ultrahigh magnetic permeability of the first metal shell and the second metal shell, the magnetic field of the magnetic assembly can be shielded and activated, so that the first state (non-working state) and the second state (working state) of the magnetic sorting mechanism can be switched, that is, by moving the first metal shell out of the magnetic assembly to be located outside the magnetic assembly through the moving mechanism, the magnetic field of the magnetic assembly can be activated, when the first metal shell returns to the inside of the magnetic assembly, the magnetic field of the magnetic assembly is shielded, and the magnetic field is deactivated, the utility model can effectively and controllably activate and deactivate the high-strength permanent magnetic field. In addition, the second metal shell always weakens the invalid magnetic field, when the magnetic sorting mechanism is in the second state, the second metal shell can weaken the invalid magnetic field and enhance the magnetic field strength of the magnetic sorting area (the area where the magnetic beads in the sorting column exist) at the same time, so as to facilitate the magnetic sorting work of cells, ensure that a uniform and high-strength magnetic field always exists in the whole cell sorting process, and be beneficial to improving the cell magnetic sorting effect.

[0008] According to the preferred embodiment of the utility model, the orthogonal projection of the sorting column, the limiting sleeve, the first metal shell, the magnetic assembly and the second metal shell on the horizontal plane is a concentric circle, the inner wall of the limiting sleeve is attached to the outer wall of the sorting column, the inner wall of the first metal shell is attached to the outer wall of the limiting sleeve, and there is a gap between the outer wall of the first metal shell and the inner wall of the magnetic assembly. In some embodiments of the utility model, under the action of external force, the first metal shell can move up and down relative to the magnetic assembly; the limiting sleeve is used for limiting and fixing the sorting column, the shape and contour of the bottom of the limiting sleeve are consistent with the shape and contour of the bottom of the sorting column, so that the sorting column is prevented from falling or being pushed out in the vertically downward direction.

[0009] According to the preferred embodiment of the utility model, the height of the sorting column is less than the height of the magnetic assembly, the heights of the limiting sleeve, the first metal shell and the magnetic assembly are equal, and the bottom surface of the sorting column is flush with the bottom surfaces of the limiting sleeve, the first metal shell and the magnetic assembly; the height of the second metal shell is greater than the height of the magnetic assembly. The height of the sorting column is less than the height of the magnetic assembly, so that the sorting column can be within the effective magnetic field range during magnetic sorting of cells, and the magnetic sorting effect is ensured.

[0010] According to the preferred embodiment of the utility model, the moving mechanism comprises a pushing table, a driver and at least two lead screws, the lead screws are perpendicular to the pushing table, the two ends of the lead screws are rotatably connected to the top and bottom of the fixed frame respectively, the rotating shaft of the driver is fixedly connected to one end of the lead screw, the first shell is fixedly connected to the bottom surface of the pushing table, the lead screw penetrates through the thickness direction of the pushing table and is rotatably connected to the pushing table, and the driver is used for driving the pushing table to move along the length direction of the lead screw.

[0011] According to the preferred embodiment of the utility model, the bottom of the second shell is fixedly connected to the bottom of the fixed frame, the shapes of the first shell and the second shell are both hollow cylinders, and the inner diameter of the second shell is greater than the outer diameters of the first shell and the first metal shell.

[0012] According to the preferred embodiment of the utility model, the top surface of the second shell is attached to the bottom surface of the magnetic assembly, and the centers of the first shell and the second shell and the center of the magnetic assembly are all located on the same extension line parallel to the lead screw. The second shell can support the magnetic sorting mechanism, and the first metal shell can be pushed out and retracted conveniently.

[0013] According to the preferred embodiment of the utility model, the outer diameter of the first shell is less than the inner diameter of the magnetic assembly and greater than the outer diameter of the limiting sleeve, and the inner diameter of the first shell is equal to or greater than the inner diameter of the first metal shell.

[0014] According to the preferred embodiment of the utility model, when the magnetic sorting mechanism is in the first state and the second state, the bottom surface of the first shell and the top surface of the first metal shell are at least partially attached.

[0015] Preferably, the inner diameter and the outer diameter of the first shell, the second shell and each mechanism in the entire magnetic sorting mechanism are designed to ensure that when the driver drives the push table to move downward along the length direction of the screw rod, the first shell can be driven to move downward to push the first metal shell and push the first metal shell out of the magnetic assembly, so that the first metal shell is located inside the second shell.

[0016] According to the preferred embodiment of the utility model, the length of the first shell and the second shell is greater than or equal to the length of the first metal shell, and when the magnetic sorting mechanism is in the second state, the first metal shell is also located inside the second shell. The length of the first shell and the second shell is greater than or equal to the length of the first metal shell, so as to ensure that the first shell can completely push the first metal shell out of the magnetic assembly and completely enter the second shell.

[0017] According to the preferred embodiment of the utility model, the material of the first metal shell and the second metal shell is nickel-iron alloy, and the material of the first shell and the second shell is non-magnetic material.

[0018] Compared with the prior art, the cell magnetic sorting device with a sorting column of the utility model has the advantages that: the magnetic assembly with a Halbach array magnet structure can provide a uniform basic magnetic field, and the synergistic effect between the first metal shell and the sorting column filled with magnetic beads can form a double magnetic field enhancement effect; by increasing the second metal shell and utilizing the cooperation of the second metal shell with the first metal shell and the moving mechanism, the activation and shielding of the high-strength permanent magnetic field can be effectively and controllably realized, so that the switching between the non-working state and the working state of the magnetic sorting mechanism is realized, the invalid magnetic field is weakened and the magnetic field strength of the sorting area is enhanced in the working state, so that a uniform and high-strength magnetic field always exists in the entire cell sorting process, which is beneficial to improve the cell magnetic sorting effect. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical scheme in the embodiments of the utility model, the drawings needed in the embodiment description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the utility model, and other drawings can be obtained by those skilled in the art without creating labor.

[0020] Figure 1 It is a cross-sectional structure schematic view of the cell magnetic sorting device in the preferred embodiment of the utility model.

[0021] Figure 2 is a sectional view structure schematic diagram of the magnetic sorting mechanism in the preferred embodiment of the utility model;

[0022] Figure 3 is Figure 2 is a sectional view schematic diagram along A-A direction;

[0023] Wherein, the sign is: fixed frame-1, magnetic sorting mechanism-2, sorting column-21, magnetic bead-211, limit sleeve-22, first metal shell-23, magnetic assembly-24, permanent magnet-241, second metal shell-25, moving mechanism-3, first shell-31, second shell-32, push table-33, driver-34, screw rod-35. DETAILED DESCRIPTION

[0024] In order to make the person in the art better understand the technical scheme of the utility model, the technical scheme in the embodiment of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiment of the utility model, obviously, the described embodiment is only a part of the embodiment of the utility model, rather than all the embodiments. Based on the embodiment in the utility model, all other embodiments obtained by the person skilled in the art without making creative labor should belong to the scope of the protection of the utility model.

[0025] Referring to Figures 1 to 3 The cell magnetic force sorting device with sorting column 21 of the embodiment includes fixed frame 1, magnetic sorting mechanism 2 and moving mechanism 3, moving mechanism 3 is installed on fixed frame 1, magnetic sorting mechanism 2 is installed on moving mechanism 3, and the height direction of fixed frame 1 is parallel to the length direction of magnetic sorting mechanism 2 and moving mechanism 3.

[0026] Further, as Figure 2As shown, the magnetic separation mechanism 2 comprises, from inside to outside, a separation column 21, a limiting sleeve 22, a first metal shell 23, a magnetic assembly 24 and a second metal shell 25, the material of the first metal shell 23 and the second metal shell 25 is nickel-iron alloy, which has super-high magnetic permeability. Among them, the first metal shell 23 and the magnetic assembly 24 are both hollow cylindrical structures, the orthogonal projection of the separation column 21, the limiting sleeve 22, the first metal shell 23, the magnetic assembly 24 and the second metal shell 25 on the horizontal plane is a concentric circle. The separation column 21 is filled with magnetic beads 211, the inner wall of the limiting sleeve 22 is attached to the outer wall of the separation column 21, and the shape and contour of the bottom of the limiting sleeve 22 is consistent with the shape and contour of the bottom of the separation column 21, so that the limiting sleeve 22 can limit and fix the separation column 21, thereby ensuring that the separation column 21 will not fall or be pushed out in the vertical downward direction. The inner wall of the first metal shell 23 is attached to the outer wall of the limiting sleeve 22, and there is a gap between the outer wall of the first metal shell 23 and the inner wall of the magnetic assembly 24, under the action of external force, the first metal shell 23 can move up and down relative to the magnetic assembly 24.

[0027] Preferably, the magnet structure of the magnetic assembly 24 is a Halbach array, the magnetic assembly 24 of the embodiment is formed by fixing four permanent magnets 241 with different magnetization directions in the second metal shell 25, the height of the second metal shell 25 is greater than the height of the magnetic assembly 24, and the top and bottom of the second metal shell 25 both have shielding parts extending inward from the outer wall thereof for fixing and limiting the magnetic assembly 24; Figures 1 to 3 The magnetic poles of each permanent magnet 241 are indicated. The height of the separation column 21 is less than the height of the magnetic assembly 24, the bottom surface of the separation column 21 is flush with the bottom surfaces of the limiting sleeve 22, the first metal shell 23 and the magnetic assembly 24, and the heights of the limiting sleeve 22, the first metal shell 23 and the magnetic assembly 24 are equal, so as to ensure that the entire separation column 21 can be within the effective magnetic field range during magnetic separation of cells, and the magnetic separation effect is guaranteed.

[0028] Further, with reference to Figure 1 The moving mechanism 3 comprises a first shell 31, a second shell 32, a push table 33, a driver 34 and at least two lead screws 35, in the embodiment, two lead screws 35 and two drivers 34 are provided, the driver 34 is preferably a motor, the lead screw 35 is perpendicular to the push table 33, the two ends of the lead screw 35 are rotatably connected to the top and bottom of the fixed frame 1, and the distance between the top and bottom of the fixed frame 1 is always kept unchanged; the rotating shaft of the driver 34 is fixedly connected to one end of the lead screw 35, the lead screw 35 penetrates through the thickness direction of the push table 33 and is rotatably connected to the push table 33, the rotating shaft of the driver 34 can drive the lead screw 35 to rotate, thereby enabling the push table 33 to move along the length direction of the lead screw 35.

[0029] Specifically, the first shell 31 is located above the second shell 32, the top surface of the first shell 31 is fixedly connected with the bottom surface of the push table 33, the bottom of the second shell 32 is fixedly connected with the bottom of the fixed frame 1, the top surface of the second shell 32 is always attached to the bottom surface of the magnetic assembly 24, and the materials of the first shell 31 and the second shell 32 are non-magnetic materials. The second shell 32 can support the magnetic separation mechanism 2. Since the first metal shell 23 and the second metal shell 25 have an attractive force with the magnetic assembly 24, when the magnetic assembly 24 is supported by the second shell 32, the stability of the first metal shell 23 and the second metal shell 25 can be ensured.

[0030] In the embodiment, the first shell 31 and the second shell 32 are both hollow cylinders, and the centers of the first shell 31 and the second shell 32 are located on the same extension line parallel to the lead screw 35. In addition, the magnetic separation mechanism 2 has a first state and a second state. When the magnetic separation mechanism 2 is in the first state, the magnetic separation mechanism 2 is located between the first shell 31 and the second shell 32, the first metal shell 23 is located inside the magnetic assembly 24, and the magnetic assembly 24 is located inside the second metal shell 25. When the magnetic separation mechanism 2 is in the second state, the first metal shell 23 is located outside the magnetic assembly 24 and inside the second shell 32, and the magnetic assembly 24 is located inside the second metal shell 25.

[0031] Further, in order to ensure that when the driver 34 drives the push table 33 to move downward along the length direction of the lead screw 35, the first shell 31 can be driven to move downward to push the first metal shell 23 and push the first metal shell 23 out of the magnetic assembly 24, so that it is located inside the second shell 32, the inner diameter of the second shell 32 is greater than the outer diameter of the first shell 31 and the first metal shell 23, the outer diameter of the first shell 31 is less than the inner diameter of the magnetic assembly 24 and greater than the outer diameter of the limiting sleeve 22, the inner diameter of the first shell 31 is equal to or greater than the inner diameter of the first metal shell 23, and the bottom surface of the first shell 31 is at least partially attached to the top surface of the first metal shell 23. The length of the first shell 31 and the second shell 32 is greater than or equal to the length of the first metal shell 23.

[0032] In the embodiment, the first metal shell 23 is pushed out of the magnetic assembly 24 and / or is retracted by driving the push table 33 and the first shell 31 to move up and down by the driving action of the driver 34, so as to realize the switching between the first state (non-working state) and the second state (working state) of the magnetic separation mechanism 2. When the sample is poured from the top of the separation column 21, the push table 33 drives the first shell 31 to push the first metal shell 23 out of the magnetic assembly 24 to the outside of the magnetic assembly 24 (at this time, in the second state), so as to realize the activation of the magnetic field of the magnetic assembly 24, and the second metal shell 25 can weaken the invalid magnetic field and enhance the magnetic field strength of the magnetic separation area, which is beneficial to improve the cell magnetic separation effect and ensure the effective separation and collection of the target cells in the sample (the sample can flow out from the bottom of the separation column 21). When the sample completes the magnetic separation in the separation column 21, the rotating shaft of the driver 34 is reversed to drive the push table 33 and the first shell 31 to move upwards, and the first metal shell 23 is not subjected to the pressure action of the first shell 31, so as to further move upwards to enter the inside of the magnetic assembly 24 (at this time, in the first state) due to the magnetic attraction of the magnetic assembly 24, so as to realize the shielding of the magnetic field of the magnetic assembly 24 and deactivate the magnetic field.

[0033] The above embodiment is only for illustrating the technical concept and characteristics of the present application, and the purpose is to enable those skilled in the art to understand the content of the present application and implement it, and cannot limit the protection scope of the present application. Any equivalent changes or modifications made according to the spirit and essence of the present application shall be covered within the protection scope of the present application.

Claims

1. A cell magnetic sorting device with a sorting column, wherein the sorting column is filled with magnetic beads, characterized in that: The device includes a fixed frame, a magnetic sorting mechanism, and a moving mechanism. The moving mechanism is mounted on the fixed frame, and the magnetic sorting mechanism is mounted on the moving mechanism. The magnetic sorting mechanism, from the inside out, includes a sorting column, a limiting sleeve, a first metal shell, a magnetic component, and a second metal shell. The magnetic component has a Helbeck array magnet structure. The moving mechanism includes a first shell and a second shell, with the first shell located above the second shell. The magnetic sorting mechanism has a first state and a second state. When the magnetic sorting mechanism is in the first state, it is located between the first and second shells, with the first metal shell inside the magnetic component and the magnetic component inside the second metal shell. When the magnetic sorting mechanism is in the second state, the first metal shell is outside the magnetic component and the magnetic component is inside the second metal shell.

2. The cell magnetic sorting device with a sorting column according to claim 1, characterized in that: The sorting column, the limiting sleeve, the first metal shell, the magnetic component, and the second metal shell are concentric circles when projected onto the horizontal plane. The inner wall of the limiting sleeve is in contact with the outer wall of the sorting column, the inner wall of the first metal shell is in contact with the outer wall of the limiting sleeve, and there is a gap between the outer wall of the first metal shell and the inner wall of the magnetic component.

3. The cell magnetic sorting device with a sorting column according to claim 2, characterized in that: The height of the sorting column is less than the height of the magnetic component, and the heights of the limiting sleeve, the first metal shell, and the magnetic component are all equal. The bottom surface of the sorting column is flush with the bottom surfaces of the limiting sleeve, the first metal shell, and the magnetic component. The height of the second metal shell is greater than the height of the magnetic component.

4. The cell magnetic sorting device with a sorting column according to claim 2, characterized in that: The moving mechanism includes a pusher, a driver, and at least two lead screws. The lead screws are perpendicular to the pusher, and their two ends are rotatably connected to the top and bottom of the fixed frame, respectively. The drive shaft is fixedly connected to one end of the lead screw. The first housing is fixedly connected to the bottom surface of the pusher. The lead screw passes through the thickness direction of the pusher and is rotatably connected to the pusher. The driver is used to drive the pusher to move along the length direction of the lead screw.

5. The cell magnetic sorting device with a sorting column according to claim 4, characterized in that: The bottom of the second housing is fixedly connected to the bottom of the fixed frame. Both the first housing and the second housing are hollow cylinders. The inner diameter of the second housing is larger than the outer diameter of the first housing and the first metal shell.

6. The cell magnetic sorting device with a sorting column according to claim 5, characterized in that: The top surface of the second housing is in contact with the bottom surface of the magnetic component, and the centers of the first housing and the second housing are located on the same extension line parallel to the lead screw as the center of the magnetic component.

7. The cell magnetic sorting device with a sorting column according to claim 6, characterized in that: The outer diameter of the first housing is smaller than the inner diameter of the magnetic component and larger than the outer diameter of the limiting sleeve. The inner diameter of the first housing is equal to or greater than the inner diameter of the first metal shell.

8. The cell magnetic sorting device with a sorting column according to claim 7, characterized in that: When the magnetic sorting mechanism is in the first state and the second state, the bottom surface of the first housing is at least partially in contact with the top surface of the first metal housing.

9. The cell magnetic sorting device with a sorting column according to claim 7, characterized in that: The lengths of both the first and second housings are greater than or equal to the length of the first metal housing. When the magnetic sorting mechanism is in the second state, the first metal housing is still located inside the second housing.

10. The cell magnetic sorting device with a sorting column according to any one of claims 1 to 9, characterized in that: Both the first and second metal shells are made of nickel-iron alloy, and both shells are made of non-magnetic materials.

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