Temperature measuring and uniform mixing device for preparing agarose gel
By designing a combination of a shaking mixing device and a thermochromic film for agarose gel preparation, the problems of low mixing efficiency and solution contamination in traditional agarose gel preparation were solved, achieving efficient and safe solution mixing and temperature control.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-04
- Publication Date
- 2026-03-27
AI Technical Summary
In the traditional agarose gel preparation process, manual mixing is cumbersome and inefficient, the solution temperature is difficult to control, and traditional temperature measuring equipment is prone to contaminating the solution, increasing operational risks.
A temperature-measuring mixing device including a shaking mixing device and a thermochromic film was designed. The container is fixed on the temperature measuring plate by a fixing device, and the temperature is monitored by the thermochromic film to avoid direct contact with the solution. Combined with shaking mixing, the temperature is ensured to be within a specified range.
This method achieves efficient solution mixing and temperature control, avoids solution contamination, reduces manual operation, and improves the convenience and safety of experiments.
Smart Images
Figure CN224040715U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to agarose gel preparation technical field, especially related to a temperature measuring and mixing device for agarose gel preparation. BACKGROUND
[0002] In the field of biological experiments, the preparation of agarose gel is a basic and critical operation, which is widely used in nucleic acid electrophoresis and many other experimental links. In the traditional preparation process of agarose gel, the experimenters need to manually mix the agarose solution with other reagents, which is tedious and inefficient. For example, when mixing the heated and melted agarose solution with buffer, dye and other reagents, it is often necessary to shake the container manually, which not only consumes a lot of manpower, but also cannot guarantee the uniformity of the mixture, which may adversely affect the experimental results.
[0003] If the temperature of the agarose solution is below 50℃, it will start to solidify, affecting the mixing of the dye and the agarose solution. However, if the temperature of the agarose solution is too high when mixed with the dye, the dye may volatilize, which not only affects the combination of the dye and the agarose solution, but also may cause the release of high toxicity of some dyes. Therefore, it is necessary to monitor the temperature of the solution during the mixing process, and quickly mix the dye with the agarose solution within a certain temperature range to avoid the solidification of the agarose solution and the rapid volatilization of the dye. According to experiments, the temperature of the agarose solution is most suitable for mixing with the dye when it is between 60-70℃.
[0004] In the past, in order to monitor the temperature of the solution, laboratories mostly used traditional thermometers, such as glass mercury thermometers or electronic thermometers. These thermometers need to be inserted directly into the solution during use. On the one hand, it is easy to contaminate the solution, especially for biological experimental samples with high purity requirements, even a small amount of contamination may interfere with the experimental data, causing the experimental results to deviate. On the other hand, frequent insertion and removal of the thermometer during the experimental operation not only increases the operation steps, but also increases the risk of human error. If not careful, the container may be knocked over, causing the solution to spill and waste, or even damaging the experimental equipment.
[0005] At the same time, in order to avoid the risk of occupational exposure, the preparation process of agarose gel is usually carried out in a biological safety cabinet. The operator needs to operate the instruments in the safety cabinet with gloves on and observe the situation in the cabinet through the glass. In this operating environment, neither manually shaking the preparation container nor using the traditional temperature monitoring device to monitor the temperature is convenient.
[0006] In summary, there is an urgent need to develop a new temperature measuring and mixing device suitable for the preparation of agarose gel to overcome the many shortcomings of the existing technology and promote the development of biological experimental technology towards more accurate, efficient and environmentally friendly directions. INVENTION CONTENTS
[0007] The utility model intends to provide a kind of temperature measuring and mixing device for agarose gel preparation, mainly for solving the technical problems that manual shaking of agarose solution and possible contamination of agarose solution caused by using traditional temperature measuring equipment in prior art.
[0008] To solve the above technical problems, the utility model provides the following technical solutions:
[0009] A kind of temperature measuring and mixing device for agarose gel preparation, including oscillation mixing device, the oscillation mixing device includes the vibration plate that can be vibrated, and the fixing device for fixing preparation container is clamped on vibration plate, the fixing device is fixedly connected with the temperature measuring plate for placing preparation container, and the upper surface of the temperature measuring plate is provided with thermochromic film.
[0010] In the present scheme, the device is placed in a biological safety cabinet, then after all accessories are assembled, the preparation container heated in microwave oven is placed on the temperature measuring plate, and then the preparation container is fixed to contact with the temperature measuring plate by the fixing device;By observing the color of thermochromic film on temperature measuring plate, it is judged whether the solution in preparation container reaches the specified temperature range, and after reaching, dye is added, then the oscillation mixing device is started, and the vibration plate starts to vibrate to mix the solution in preparation container, and the color of thermochromic film is paid attention to at all times during mixing, and the oscillation mixing device is closed before the solution temperature deviates from the specified temperature range, and the preparation container is taken out, and the mixed solution is poured into the gel preparation plate.
[0011] The advantages of the present scheme are:
[0012] 1. In order to avoid contamination of agarose solution, the existing laboratory equipment (i.e. oscillation mixing device) is improved, and the fixing device is added to the oscillation mixing device to fix the preparation container on the temperature measuring plate, and the solution is shaken under the vibration by starting the oscillation mixing device, which reduces the artificial burden and does not contaminate the solution.
[0013] 2. The temperature measurement of the solution is completed by thermochromic film, which does not directly contact the solution during temperature measurement, avoiding contamination of the solution, and because the solution only needs to be kept in a temperature range rather than an accurate temperature, high-cost accurate temperature measuring equipment is not needed, and thermochromic film is used to detect whether the temperature is in the set range.
[0014] 3. The whole preparation process is completed in a biological safety cabinet, and only the discoloration of thermochromic film needs to be observed during temperature measurement, without the need for reading, which is convenient to use.
[0015] In summary, the present scheme solves the technical problems that manual shaking of agarose solution and possible contamination of agarose solution caused by using traditional temperature measuring equipment in prior art.
[0016] Preferably, the fixing device includes a snap-fit assembly snapping onto the vibrating plate. The snap-fit assembly includes snap-fit blocks fixed at the four corners of the vibrating plate. A connecting assembly is provided between adjacent snap-fit blocks. The connecting assembly includes a first snap-fit plate and a second snap-fit plate. The first snap-fit plate has a strip groove. The second snap-fit plate has a slider that can be inserted into and slide within the strip groove. A limit nut is threaded to the end of the slider away from the second snap-fit plate. The ends of the first and second snap-fit plates that are away from each other are respectively fixed to adjacent snap-fit blocks. In this solution, the four snap-fit blocks are first pre-connected through the connecting assembly, then the four snap-fit blocks are snapped onto the four corners of the vibrating plate, and then locked by the connecting assembly to fix the relative positions of adjacent snap-fit blocks. The pre-connection and locking steps of adjacent snap-fit blocks are as follows: the slider is inserted into the strip groove, and then the adjacent snap-fit blocks can be pulled tight by pulling the first and second snap-fit plates. Then, the limit nut is tightened so that the first and second snap-fit plates are pressed tightly against each other and cannot slide against each other.
[0017] Preferably, the fixing device includes a clamping component fixed to the snap-fit assembly. The clamping component includes a prism disposed on the upper surface of the snap-fit block. A fixing component for securing the preparation container and preventing horizontal displacement of the preparation container is sleeved on the prism. A spring is sleeved on the prism, with one end of the spring fixed to the upper surface of the snap-fit block and the other end fixedly connected to the lower surface of the fixing component. In this solution, after the preparation container is removed from the microwave oven and placed in the biosafety cabinet, the fixing component is pulled upwards, and then the preparation container is placed below the fixing component. The fixing component is then lowered to secure the preparation container and prevent horizontal displacement. Then, the shaking and mixing device is activated to shake and mix the solution in the preparation container.
[0018] Preferably, the temperature measuring plate is fixedly connected to the card block, and the lower surface of the temperature measuring plate is in close contact with the vibration plate. A soft layer is provided on the surface of the temperature measuring plate, and the thermochromic film is attached to the upper surface of the soft layer. In this design, the soft layer prevents the temperature measuring plate from forming hard contact with the preparation container, thus preventing damage to the preparation container during vibration.
[0019] Preferably, the fixing component includes a fixing arm sleeved on the prism, the fixing arm being fixedly connected to a spring, and a fixing ring for securing the preparation container being fixed to the side of the fixing arm. In this design, the fixing ring can encircle the preparation container, and when the preparation container is a conical flask, the fixing ring can press against the conical surface of the conical flask, making the conical flask more stably fixed.
[0020] Preferably, the surface of the fixing ring is coated with a rubber layer. In this design, the rubber layer can prevent the fixing ring from making hard contact with the preparation container during vibration, thus preventing damage to the preparation container. At the same time, the rubber layer can also increase the friction with the preparation container, preventing the preparation container from coming off the fixing component during vibration.
[0021] Preferably, the limiting nut surface is fixed with a holding part for facilitating manual tightening of the limiting nut. BRIEF DESCRIPTION OF DRAWINGS
[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments will be briefly introduced as follows. In all the drawings, similar elements or parts are generally identified by similar reference signs. In the drawings, each element or part is not necessarily drawn according to the actual proportion.
[0023] Figure 1 It is a perspective view of the temperature measuring and mixing device for agarose gel preparation of the utility model patent;
[0024] Figure 2 It is a fixing device structure view of the temperature measuring and mixing device for agarose gel preparation of the utility model patent;
[0025] Figure 3 It is a perspective view of the temperature measuring and mixing device for agarose gel preparation of the utility model patent; Figure 2 It is an enlarged view of A of the temperature measuring and mixing device for agarose gel preparation;
[0026] Figure 4 It is a schematic view of the oscillation mixing machine of the temperature measuring and mixing device for agarose gel preparation of the utility model patent;
[0027] Figure 5 It is a schematic view of the conical flask of the temperature measuring and mixing device for agarose gel preparation of the utility model patent.
[0028] The reference signs in the drawings of the specification include: 1, oscillation mixing machine; 21, clamping block; 22, prism; 23, spring; 241, fixing arm; 242, fixing ring; 25, temperature measuring plate; 31, second clamping plate; 32, first clamping plate; 33, sliding block; 34, limiting nut. DETAILED DESCRIPTION
[0029] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0030] In the description of the utility model, it is necessary to explain that the orientation or positional relation indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "top", "bottom", "top surface", "bottom surface", "inner", "outer", "inner side", "outer side" and the like is the orientation or positional relation shown based on the drawings, and is only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as limiting the utility model.
[0031] In the description of the utility model, the meaning of several is one or more, and the meaning of multiple is more than two, and greater than, less than, more than and the like are not included in the number, and above, below, within and the like are included in the number. If the terms "first", "second", "third" are described, they are only for the purpose of description and distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or the sequence of indicated technical features.
[0032] In the description of the utility model, it is necessary to explain that, unless otherwise specified and limited, the terms "mounting", "connection", "connection", "setting" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication between two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances. The embodiments will be described below according to the overall structure of the utility model.
[0033] As shown in Figure 1 The temperature measuring and mixing device for preparing agarose gel of the utility model takes the commonly used oscillation mixer 1 in the laboratory as the power center (as shown in Figure 1 and Figure 4 The device is designed in combination with the agarose solution preparation method to avoid solution pollution during preparation and real-time monitoring of solution temperature.
[0034] As shown in Figure 1 The oscillation mixer 1 comprises a vibratable vibration plate, and clamping blocks 21 are fixed at the four corners of the vibration plate, the two side surfaces of the clamping blocks 21 are at right angles, and connecting assemblies are arranged between adjacent clamping blocks 21; as Figure 3As shown, the connecting assembly comprises a first clamping plate 32 and a second clamping plate 31, the first clamping plate 32 is provided with a strip-shaped slot, the second clamping plate 31 is fixed with a sliding block 33 which can be inserted into the strip-shaped slot and slide in the strip-shaped slot, the end of the sliding block 33 away from the second clamping plate 31 is threadedly connected with a limiting nut 34, the surface of the limiting nut 34 is fixed with a holding part which is convenient for manually tightening the limiting nut 34; the ends of the first clamping plate 32 and the second clamping plate 31 away from each other are respectively fixed on the adjacent clamping blocks 21.
[0035] In use, first, the four clamping blocks 21 are pre-connected through the connecting assembly, then the four clamping blocks 21 are buckled on the four corners of the vibration plate, then the relative positions of the adjacent clamping blocks 21 are fixed through locking by the connecting assembly, the pre-connection and locking steps of the adjacent clamping blocks 21 are as follows: the sliding block 33 is inserted into the strip-shaped slot, then the adjacent clamping blocks 21 are pulled tight by pulling the first clamping plate 32 and the second clamping plate 31, so that the clamping blocks 21 are tightly attached to the vibration plate, then the first clamping plate 32 and the second clamping plate 31 are tightly pressed and cannot slide relative to each other by tightening the limiting nut 34.
[0036] As shown in Figure 1 , Figure 2 , the upper surface of the clamping block 21 is fixed with a prism 22, the prism 22 is sleeved with a fixing component for sleeving the preparation container and preventing the horizontal displacement of the preparation container, the prism 22 is sleeved with a spring 23, one end of the spring 23 is fixed with the upper surface of the clamping block 21, and the other end is fixedly connected with the lower surface of the fixing component; when the preparation container is taken out from the microwave oven and placed in the biological safety cabinet, the fixing component is pulled upward, then the preparation container is placed below the fixing component, then the fixing component falls to cover the preparation container to prevent the horizontal displacement of the preparation container, then the solution in the preparation container is mixed and shaken by opening the shaking and mixing device.
[0037] The fixing component comprises a fixing arm 241 sleeved on the prism 22, the fixing arm 241 is fixedly connected with the spring 23, and the fixing arm 241 is fixed with a fixing ring 242 on the side surface for sleeving the preparation container; the fixing ring 242 can enclose the preparation container, and the surface of the fixing ring 242 is attached with a rubber layer, which can prevent the preparation container from being damaged due to hard contact between the fixing ring 242 and the preparation container during the shaking process, and at the same time, the rubber layer can also increase the friction force with the preparation container to prevent the preparation container from being taken out of the fixing component during the shaking process.
[0038] The temperature measuring plate 25 is fixedly connected to the clamping block 21, and the lower surface of the temperature measuring plate 25 is tightly attached to the vibration plate. A soft sponge cloth (the thickness of the sponge cloth is preferably 1 mm) is arranged on the surface of the temperature measuring plate 25, and the thermochromic film is attached to the upper surface of the sponge cloth. The sponge cloth can prevent the temperature measuring plate 25 from being in hard contact with the preparation container and damaging the preparation container during vibration. The thermochromic film can be an inorganic thermochromic film or a high polymer thermochromic film. When the temperature of the bottom of the preparation container is 60-75°C, the thermochromic film can display a unique and conspicuous color.
[0039] The preparation container in the embodiment is a conical flask (as shown in Figure 5 The fixing ring 242 can compress the conical surface of the conical flask, so that the conical flask is fixed more stably.
[0040] The technical principle of the embodiment is as follows:
[0041] In the biological safety cabinet, the four clamping blocks 21 are pre-connected through the connecting assembly, that is, the sliding block 33 is inserted into the strip-shaped slot, and then the four clamping blocks 21 are buckled at the four corners of the vibration plate. By pulling the first clamping plate 32 and the second clamping plate 31, the adjacent clamping blocks 21 can be pulled tightly, so that the clamping blocks 21 are tightly attached to the vibration plate. Then, by tightening the limiting nut 34, the first clamping plate 32 and the second clamping plate 31 are tightly pressed and cannot slide relative to each other.
[0042] In the laboratory, an appropriate amount of agarose powder is weighed and added to the conical flask, and an appropriate amount of buffer is added to the conical flask. Then, the conical flask is placed in a microwave oven for heating. After heating, the conical flask is placed in a biological safety cabinet, the fixing ring 242 is lifted upwards, the conical flask is placed on the temperature measuring plate 25 and contacts the thermochromic film, and then the fixing ring 242 is lowered to cover the conical flask and press the conical surface of the conical flask. The conical flask is stably fixed on the shaking mixer 1.
[0043] The color change of the thermochromic film is observed. When the thermochromic film changes to a specified color, it is proved that the solution in the conical flask has been cooled to a specified temperature range. Then, nucleic acid dye is added to the conical flask, the shaking mixer 1 is started to shake, and the color change of the thermochromic film in contact with the conical flask is observed at any time to ensure that the shaking and mixing are stopped before the color of the thermochromic film changes to the color corresponding to the temperature outside the specified temperature, the shaking mixer 1 is turned off, the fixing ring 242 is lifted, the conical flask is removed, and the mixed solution is poured into the gel preparation plate.
[0044] The foregoing description of the specific exemplary embodiments of the present application is for the purpose of illustrating and describing, rather than limiting the application, and it will be apparent to those of ordinary skill in the art that many changes and modifications can be made to the embodiments with the foregoing teaching, notwithstanding what can be suggested above and although only a few of the exemplary embodiments of the present application have been described. Although the embodiments of the present application have been described, the specific embodiments are merely illustrative of the present application and are not intended to limit the present application, and the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner, and the exemplary embodiments are selected and described for the purpose of explaining the specific principles of the present application and its practical application, so that those skilled in the art can make modifications, replacements, variations and various different selections and changes to the embodiments without creative contribution after reading the specification, as long as they are within the scope of the claims of the present application.
Claims
1. A temperature measuring and mixing device for preparing an agarose gel, characterized in that, The utility model provides a mixing device for preparation container, which comprises a shaking mixing device, the shaking mixing device comprises a shakable vibration plate, a fixing device for fixing the preparation container is clamped on the vibration plate, a temperature measuring plate for placing the preparation container is fixedly connected to the fixing device, and a thermochromic film is arranged on the upper surface of the temperature measuring plate.
2. The temperature measuring and mixing device for preparing agarose gel according to claim 1, characterized in that, The fixing device comprises a clamping assembly clamped on the vibration plate, the clamping assembly comprises clamping blocks fixed at four corners of the vibration plate, connecting assemblies are arranged between adjacent clamping blocks, the connecting assemblies comprise first clamping plates and second clamping plates, strip-shaped grooves are formed in the first clamping plates, sliding blocks are fixed to the second clamping plates and can be inserted into the strip-shaped grooves and slide in the strip-shaped grooves, and limit nuts are threadedly connected to one ends of the sliding blocks away from the second clamping plates; and one ends of the first clamping plates and the second clamping plates away from each other are fixed to adjacent clamping blocks respectively.
3. The temperature measuring and mixing device for preparing agarose gel according to claim 2, characterized in that, The fixing device comprises clamping assemblies fixed to the clamping assembly, the clamping assemblies comprise prisms arranged on upper surfaces of the clamping blocks, fixing components for sleeving the preparation containers and preventing horizontal displacement of the preparation containers are sleeved on the prisms, springs are sleeved on the prisms, one ends of the springs are fixed to the upper surfaces of the clamping blocks, and the other ends of the springs are fixedly connected to lower surfaces of the fixing components.
4. The temperature measuring and mixing device for preparing agarose gel according to claim 1, characterized in that, The temperature measuring plate is fixedly connected to the clamping blocks, the lower surface of the temperature measuring plate is tightly attached to the vibration plate, a soft layer with soft texture is arranged on the surface of the temperature measuring plate, and the thermochromic film is attached to the upper surface of the soft layer.
5. The temperature measuring and mixing device for preparing agarose gel according to claim 3, characterized in that, The fixing component comprises a fixing arm sleeved on the prism, the fixing arm is fixedly connected to the spring, and a fixing ring for sleeving the preparation container is fixed to the side surface of the fixing arm.
6. The temperature measuring and mixing device for preparing agarose gel according to claim 5, characterized in that, A rubber layer is attached to the surface of the fixing ring.
7. The temperature measuring and mixing device for preparing agarose gel according to claim 5, characterized in that, A holding part for facilitating manual tightening of the limit nut is fixed to the surface of the limit nut.