Purification device for recombinant collagen production

Abstract

The utility model discloses a purification device for recombinant collagen production, which relates to the technical field of recombinant collagen production, and comprises a box body, the lower end of the box body is fixedly connected with a collecting hopper, the outer side of the collecting hopper is fixedly connected with a connecting pipe, the other end of the connecting pipe is fixedly connected with a filter box, and the filter box is fixedly connected with a filter screen. The upper end of the filtering box is fixedly connected with a supporting frame, the upper end of the supporting frame is fixedly connected to the outer side of the box body, and a stirring assembly is arranged in an inner cavity of the box body. At the moment, through transmission cooperation of a transmission gear, a second bevel gear can conveniently drive a second stirring shaft to rotate reversely, then a plurality of stirring rods can rotate forwards and backwards to stir a cell solution of recombinant collagen in the box body, and it is ensured that raw materials are fully mixed.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of recombinant collagen production, especially relates to a purification device for recombinant collagen production. BACKGROUND

[0002] Recombinant collagen, as a kind of bioactive material with wide application prospect, plays a key role in medicine, cosmetics, food and many other fields.

[0003] However, the existing purification device for recombinant collagen production in the raw material processing stage, since the raw material of recombinant collagen is usually cell culture solution containing recombinant genes, the composition is extremely complex, including cell debris, unreacted culture medium components, impurities, nucleic acids and endotoxins, and the traditional device often lacks efficient stirring and cell crushing synergistic mechanism, so that the recombinant collagen in the cell cannot be fully released, and the impurities are difficult to uniformly disperse, which greatly affects the subsequent purification effect, secondly, in the filtering stage, the existing filtering assembly is difficult to replace and maintain conveniently, when the filtering assembly is blocked or the filtering effect is reduced, a large amount of time and manpower is needed to disassemble, clean or replace, which seriously affects the production efficiency, in order to solve the above problems, we propose a purification device for recombinant collagen production. SUMMARY

[0004] The main purpose of the utility model is to provide a purification device for recombinant collagen production, which can effectively solve the problems in the background art.

[0005] To achieve the above purpose, the technical scheme adopted by the utility model is:

[0006] A purification device for recombinant collagen production, comprising a box body, a collecting hopper is fixedly connected to the lower end of the box body, a connecting pipe is fixedly connected to the outer side of the collecting hopper, the other end of the connecting pipe is fixedly connected with a filter box, a supporting frame is fixedly connected to the upper end of the filter box, the supporting frame is fixedly connected to the outer side of the box body, a stirring assembly is arranged in the inner cavity of the box body, an ultrasonic cell disrupter is arranged on the outer side of the stirring assembly, the ultrasonic cell disrupter is fixedly installed on the inner wall of the box body, a filtering assembly is arranged on the inner side of the filter box, a discharge pipe is fixedly connected to one side of the filter box, the discharge pipe is located below the filtering assembly, a feeding port is formed in the upper end of the box body, a limiting assembly is arranged on one side of the filter box, and the filtering assembly is fixedly connected to the inner side of the filter box through the limiting assembly.

[0007] Preferably, the stirring assembly comprises a driving motor, the driving motor is arranged at the upper end of the box body, the output end of the driving motor is fixedly connected with an upper stirring shaft, the upper stirring shaft is rotatably connected with the box body, the lower end of the upper stirring shaft is fixedly connected with a first bevel gear, the outer side of the upper stirring shaft is fixedly connected with a plurality of stirring rods, and the plurality of stirring rods are arranged in the inner cavity of the box body.

[0008] Preferably, the inner cavity of the box body is fixedly connected with a mounting seat, one side of the mounting seat is rotatably connected with a transmission gear, the transmission gear is meshedly connected with the first bevel gear, the outer side of the transmission gear is also meshed with a second bevel gear, the lower end of the second bevel gear is fixedly connected with a second stirring shaft, the second stirring shaft is rotatably connected with the lower end of the inner wall of the box body, and the outer side of the second stirring shaft is also fixedly connected with a plurality of stirring rods, and the plurality of stirring rods are arranged in the inner cavity of the box body.

[0009] Preferably, the filtering assembly comprises a filtering frame, the filtering frame is slidably connected to the inner side of the filtering box, one side of the filtering frame is fixedly connected with a handle, the handle is arranged on the outer side of the filtering box, the inner side of the filtering frame is fixedly installed with a microfiltration membrane filter screen, and the lower end of the microfiltration membrane filter screen is provided with an ultrafiltration membrane filter screen fixedly installed on the filtering frame.

[0010] Preferably, the limiting assembly comprises a sleeve, the sleeve is fixedly installed on one side of the filtering box, the inner side of the sleeve is slidably connected with a limiting rod, the outer side of the limiting rod is fixedly connected with a circular plate, the circular plate is slidably connected to the inner side of the sleeve, one side of the circular plate is fixedly connected with a spring, the other end of the spring is fixedly connected with the sleeve, one end of the limiting rod is fixedly connected with a pull ring, the pull ring is arranged on the outer side of the sleeve, the outer side of the filtering frame is provided with a limiting hole, and the other end of the limiting rod is arranged in the inner cavity of the limiting hole.

[0011] Preferably, the outer side of the connecting pipe is provided with a valve.

[0012] Preferably, the outer side of the driving motor is fixedly connected with a mounting block, and the mounting block is fixedly installed at the upper end of the box body.

[0013] Compared with the prior art, the utility model has the advantages of the following:

[0014] 1. The reconstituted collagen production purification device, the output end of the driving motor drives the upper stirring shaft to rotate, the upper stirring shaft rotates to drive the first bevel gear at the lower end to rotate, at this time, the transmission cooperation of the transmission gear can conveniently realize the reverse rotation of the second stirring shaft driven by the second bevel gear, thereby realizing the forward and reverse rotation of the plurality of stirring rods to stir the cell solution of the reconstituted collagen in the box, ensuring that the raw materials are fully mixed, while stirring, the ultrasonic disrupter fixedly installed on the inner wall of the box is started, the ultrasonic disrupter utilizes the cavitation effect of ultrasonic waves to crush the cells in the raw materials, so that the reconstituted collagen in the cells is fully released into the solution, and the synergistic effect of stirring and ultrasonic crushing improves the release efficiency and uniformity of the reconstituted collagen.

[0015] 2. The reconstituted collagen production purification device, when it is necessary to replace or maintain the filter assembly, the limit rod slides in the sleeve by pulling the pull ring, the circular plate on the outer side of the limit rod slides and compresses the spring, until the other end of the limit rod is out of the limit hole formed on the outer side of the filter frame, at this time, the limit between the filter frame and the filter box is released, the filter frame can be conveniently pulled out from the inner side of the filter box for cleaning, replacing the filter screen and other maintenance operations. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the overall structure schematic of the reconstituted collagen production purification device of the utility model Figure 1 ;

[0017] Figure 2 It is the overall structure schematic of the reconstituted collagen production purification device of the utility model Figure 2 ;

[0018] Figure 3 It is the overall structure sectional view of the reconstituted collagen production purification device of the utility model;

[0019] Figure 4 It is the partial explosion structure schematic of the reconstituted collagen production purification device of the utility model;

[0020] Figure 5 It is the enlarged structure schematic of place A of the reconstituted collagen production purification device of the utility model.

[0021] In the figure: 1, box; 2, support frame; 3, collecting hopper; 4, connecting pipe; 5, valve; 6, filter box; 7, filter assembly; 71, filter frame; 72, microfiltration membrane filter screen; 73, ultrafiltration membrane filter screen; 8, handle; 9, limiting assembly; 91, sleeve; 92, limiting rod; 93, round plate; 94, spring; 95, pull ring; 96, limiting hole; 10, driving motor; 11, mounting block; 12, upper stirring shaft; 13, first bevel gear; 14, transmission gear; 15, second bevel gear; 16, second stirring shaft; 17, stirring rod; 18, discharge pipe; 19, ultrasonic crusher. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, purposes and effects of the utility model easy to understand, the utility model is further described below in combination with specific embodiments.

[0023] As shown in Figures 1-5 A kind of purification device for recombination collagen production, including box 1, the lower end of box 1 is fixedly connected with collecting hopper 3, the outer side of collecting hopper 3 is fixedly connected with connecting pipe 4, another end of connecting pipe 4 is fixedly connected with filter box 6, the upper end of filter box 6 is fixedly connected with support frame 2, the upper end of support frame 2 is fixedly connected on the outer side of box 1, the inner cavity of box 1 is provided with stirring assembly, the outer side of stirring assembly is provided with ultrasonic crusher 19, ultrasonic crusher 19 is fixedly installed on the inner wall of box 1, the inner side of filter box 6 is provided with filter assembly 7, one side of filter box 6 is fixedly connected with discharge pipe 18, discharge pipe 18 is below filter assembly 7, the upper end of box 1 is provided with feed inlet, one side of filter box 6 is provided with limiting assembly 9, filter assembly 7 is fixedly clamped on the inner side of filter box 6 by limiting assembly 9.

[0024] In the embodiment, stirring assembly includes driving motor 10, driving motor 10 is located at the upper end of box 1, the output end of driving motor 10 is fixedly connected with upper stirring shaft 12, upper stirring shaft 12 is rotatably connected with box 1, the lower end of upper stirring shaft 12 is fixedly connected with first bevel gear 13, the outer side of upper stirring shaft 12 is fixedly connected with multiple stirring rods 17, multiple stirring rods 17 are all located in the inner cavity of box 1, mounting seat is fixedly connected on the inner cavity of box 1, transmission gear 14 is rotatably connected on the side of mounting seat, transmission gear 14 is meshingly connected with first bevel gear 13, the outer side of transmission gear 14 is also meshed with second bevel gear 15, the lower end of second bevel gear 15 is fixedly connected with second stirring shaft 16, second stirring shaft 16 is rotatably connected with the lower end of the inner wall of box 1, the outer side of second stirring shaft 16 is also fixedly connected with multiple stirring rods 17, multiple stirring rods 17 are all located in the inner cavity of box 1.

[0025] Specifically, through the feeding port opened on the upper end of the box body 1, the cell solution containing recombinant collagen or other related mixture raw materials can be poured into the inner cavity of the box body 1. At this time, the driving motor 10 is started, and the output end of the driving motor 10 drives the upper stirring shaft 12 to rotate. The rotation of the upper stirring shaft 12 drives the first bevel gear 13 at the lower end to rotate. At this time, through the transmission cooperation of the transmission gear 14, the second bevel gear 15 can conveniently drive the second stirring shaft 16 to rotate in the opposite direction, so that the plurality of stirring rods 17 can rotate in the opposite direction to stir the cell solution of the recombinant collagen in the box body 1, and ensure that the raw materials are fully mixed. At the same time of stirring, the ultrasonic crusher 19 fixedly installed on the inner wall of the box body 1 is started. The ultrasonic crusher 19 uses the cavitation effect of ultrasonic waves to crush the cells in the raw materials, so that the recombinant collagen in the cells is fully released into the solution. The synergistic effect of stirring and ultrasonic crushing improves the release efficiency and uniformity of the recombinant collagen.

[0026] In the embodiment, the filter assembly 7 includes a filter frame 71 which is slidingly connected to the inner side of the filter box 6. One side of the filter frame 71 is fixedly connected with a handle 8 which is arranged on the outer side of the filter box 6. The inner side of the filter frame 71 is fixedly installed with a microfiltration membrane filter screen 72. The microfiltration membrane filter screen 72 is provided at the lower end with an ultrafiltration membrane filter screen 73 which is fixedly installed on the filter frame 71.

[0027] Specifically, through the design of the connecting pipe 4, the solution can be conveniently poured into the filter box 6. After the solution enters the filter box 6, it first contacts the microfiltration membrane filter screen 72 for preliminary filtration, which can effectively remove larger particle impurities such as incompletely crushed cell fragments and large insoluble substances. Then, the solution is filtered again by the ultrafiltration membrane filter screen 73 to block relatively large molecular weight impurities such as part of the albumin and nucleic acid, while allowing the recombinant collagen solution to pass through, thereby achieving preliminary purification. After filtration, the solution will flow out of the filter box 6 through the discharge pipe 18 for collection for further purification treatment.

[0028] In the embodiment, the limiting assembly 9 includes a sleeve 91 which is fixedly installed on one side of the filter box 6. The inner side of the sleeve 91 is slidingly connected with a limiting rod 92. The outer side of the limiting rod 92 is fixedly connected with a circular plate 93 which is slidingly connected to the inner side of the sleeve 91. One side of the circular plate 93 is fixedly connected with a spring 94, and the other end of the spring 94 is fixedly connected with the sleeve 91. One end of the limiting rod 92 is fixedly connected with a pull ring 95 which is arranged on the outer side of the sleeve 91. The outer side of the filter frame 71 is provided with a limiting hole 96, and the other end of the limiting rod 92 is arranged in the inner cavity of the limiting hole 96.

[0029] Specifically, after a period of filtration, the filter assembly 7 will need to be replaced or maintained, at which time the operator pulls the pull ring 95, the limiting rod 92 slides in the sleeve 91, the round plate 93 outside the limiting rod 92 slides and compresses the spring 94, until the other end of the limiting rod 92 is out of the limiting hole 96 outside the filter frame 71, at this time, the limiting between the filter frame 71 and the filter box 6 is removed, the operator can pull the handle 8, the filter frame 71 is pulled out from the inside of the filter box 6, and the maintenance operation such as cleaning and replacing the filter screen is carried out, conversely, the filter frame 71 is slid into the inside of the filter box 6 again, the pull ring 95 is loosened, under the action of the spring 94, the round plate 93 drives the limiting rod 92 to reset, the other end of the limiting rod 92 is inserted into the inner cavity of the limiting hole 96 again, and the filter assembly 7 is fixed to the inside of the filter box 6.

[0030] In this embodiment, the outer side of the connecting pipe 4 is provided with a valve 5.

[0031] Specifically, through the design of the valve 5, the worker can control the flow rate of the solution in the connecting pipe 4 as needed, which is more convenient.

[0032] In this embodiment, the outer side of the driving motor 10 is fixedly connected with a mounting block 11, and the mounting block 11 is fixedly installed at the upper end of the box body 1.

[0033] Specifically, through the design of the mounting block 11, the stability of the driving motor 10 during work can be improved.

[0034] It needs explaining, the utility model discloses a kind of purification device for recombinant collagen production, user first passes through the feed inlet being opened on the upper end of box 1, can the cell solution or other related mixture raw materials containing recombinant collagen is put into the inner cavity of box 1, at this time, start drive motor 10, the output end of drive motor 10 will drive upper stirring shaft 12 to rotate, upper stirring shaft 12 rotation will drive the rotation of first bevel gear 13 at lower end, at this time, by the transmission cooperation of transmission gear 14, it can be easily realized that second bevel gear 15 drives second stirring shaft 16 to rotate reversely, and then multiple stirring rods 17 can be realized to rotate reversely in recombinant collagen's cell solution in box 1 is stirred, ensure that raw materials are mixed fully, while stirring, start ultrasonic disrupter 19 fixedly installed on the inner wall of box 1, ultrasonic disrupter 19 utilizes the cavitation effect of ultrasonic wave, and the cell in raw materials is broken, so that recombinant collagen in cell is fully released into solution, the synergistic effect of stirring and ultrasonic disruption improves the release efficiency and uniformity of recombinant collagen, at this time, valve 5 arranged outside connecting pipe 4 is opened, can realize that solution flows to filter box 6 through connecting pipe 4, after solution enters filter box 6, first contact microfiltration membrane filter screen 72 and carry out preliminary filtration, can effectively remove the cell debris that is not completely broken, large insoluble substances and other larger particle impurities, then in ultrafiltration membrane filter screen 73 again filtration, relatively large molecular mass impurities, such as part of impurity protein, nucleic acid are blocked, while allowing recombinant collagen solution to pass through, realize preliminary purification, after filtration, solution will flow out of filter box 6 through discharge pipe 18, so as to be collected for subsequent further purification treatment, after a period of filtration, filter assembly 7 will need to be replaced or maintained, at this time, operator pulls pull ring 95, so that limiting rod 92 slides in sleeve 91, round plate 93 outside limiting rod 92 slides and compresses spring 94, until the other end of limiting rod 92 is out of limiting hole 96 arranged outside filter frame 71, at this time, the limit between filter frame 71 and filter box 6 is removed, and the operator can pull handle 8 to pull filter frame 71 out of the inside of filter box 6, and perform cleaning, replace filter screen and other maintenance operations, conversely, filter frame 71 is slid into the inside of filter box 6 again, and the round plate 93 drives the limiting rod 92 to reset under the action of the spring 94, the other end of the limiting rod 92 is inserted into the inner cavity of the limiting hole 96 again, the filter assembly 7 is fixed to the inside of the filter box 6, and it is more practical.

[0035] The basic principle and main features of the present application and the advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A purification device for recombinant collagen production comprising a cabinet (1), characterized in that: The lower end of the box (1) is fixedly connected with a collecting hopper (3), the outer side of the collecting hopper (3) is fixedly connected with a connecting pipe (4), the other end of the connecting pipe (4) is fixedly connected with a filter box (6), the upper end of the filter box (6) is fixedly connected with a support frame (2), the upper end of the support frame (2) is fixedly connected to the outer side of the box (1), the inner cavity of the box (1) is provided with a stirring assembly, the outer side of the stirring assembly is provided with an ultrasonic crusher (19), the ultrasonic crusher (19) is fixedly installed on the inner wall of the box (1), the inner side of the filter box (6) is provided with a filter assembly (7), one side of the filter box (6) is fixedly connected with a discharge pipe (18), the discharge pipe (18) is located below the filter assembly (7), the upper end of the box (1) is provided with a feeding port, one side of the filter box (6) is provided with a limiting assembly (9), and the filter assembly (7) is fixedly clamped on the inner side of the filter box (6) through the limiting assembly (9).

2. The purification device for producing recombinant collagen according to claim 1, characterized in that: The stirring assembly comprises a driving motor (10), the driving motor (10) is arranged at the upper end of the box (1), the output end of the driving motor (10) is fixedly connected with an upper stirring shaft (12), the upper stirring shaft (12) is rotatably connected with the box (1), the lower end of the upper stirring shaft (12) is fixedly connected with a first bevel gear (13), the outer side of the upper stirring shaft (12) is fixedly connected with a plurality of stirring rods (17), and the plurality of stirring rods (17) are arranged in the inner cavity of the box (1).

3. The purification device for producing recombinant collagen according to claim 2, characterized in that: The inner cavity of the box (1) is fixedly connected with a mounting seat, one side of the mounting seat is rotatably connected with a transmission gear (14), the transmission gear (14) is meshedly connected with the first bevel gear (13), the outer side of the transmission gear (14) is also meshed with a second bevel gear (15), the lower end of the second bevel gear (15) is fixedly connected with a second stirring shaft (16), the second stirring shaft (16) is rotatably connected with the lower end of the inner wall of the box (1), and the outer side of the second stirring shaft (16) is also fixedly connected with a plurality of stirring rods (17).

4. The purification device for producing recombinant collagen according to claim 1, characterized in that: The filter assembly (7) comprises a filter frame (71), the filter frame (71) is slidably connected to the inner side of the filter box (6), one side of the filter frame (71) is fixedly connected with a handle (8), the handle (8) is arranged on the outer side of the filter box (6), the inner side of the filter frame (71) is fixedly installed with a microfiltration membrane filter screen (72), and the lower end of the microfiltration membrane filter screen (72) is provided with an ultrafiltration membrane filter screen (73) fixedly installed on the filter frame (71).

5. The purification device for producing recombinant collagen according to claim 4, characterized in that: Said limiting assembly (9) includes sleeve (91), sleeve (91) is fixedly installed on one side of filter box (6), the inner side of sleeve (91) is slidably connected with limiting rod (92), the outer side of limiting rod (92) is fixedly connected with round plate (93), the inner side of sleeve (91) is slidably connected with round plate (93), one side of round plate (93) is fixedly connected with spring (94), the other end of spring (94) is fixedly connected with sleeve (91), one end of limiting rod (92) is fixedly connected with pull ring (95), pull ring (95) is arranged on the outer side of sleeve (91), the outer side of filter frame (71) is provided with limiting hole (96), the other end of limiting rod (92) is arranged in the inner cavity of limiting hole (96).

6. The purification device for producing recombinant collagen according to claim 1, characterized in that: The outer side of the connecting pipe (4) is provided with a valve (5).

7. The purification device for producing recombinant collagen according to claim 2, characterized in that: The outer side of the driving motor (10) is fixedly connected with the mounting block (11), and the mounting block (11) is fixedly installed on the upper end of the box body (1).

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