Stem cell blending device
By designing a stem cell mixing device with multiple sets of stirring components and cell sieves, the problems of insufficient mixing and flocculent matter in existing technologies have been solved, achieving efficient and uniform mixing of stem cells and removal of flocculent matter, thereby improving the uniformity and stability of cell culture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-28
- Publication Date
- 2026-03-27
AI Technical Summary
In the current technology, insufficient mixing during stem cell culture and expansion leads to an uneven cell microenvironment, affecting cell activity and proliferation efficiency, and also results in the presence of stem cell flocculent material.
A stem cell mixing device was designed, comprising multiple stirring components and a cell sieve. Through multiple stirring and filtration processes, the cells are ensured to be uniformly mixed and flocculent matter is removed. The device includes a first, second, and third stirring component and a perforated baffle. Stirring is performed using a motor-driven rotating shaft and stirring blades, combined with a conical structure and cell sieve design.
This method achieves efficient and uniform mixing of stem cells, removes most of the flocculent material, improves the uniformity and stability of cell culture, and ensures the stability of cell activity and proliferation efficiency.
Smart Images
Figure CN224047362U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to a stem cell mixing device. BACKGROUND
[0002] In the stem cell culture, amplification and biological manufacturing process, the lack of mixing degree directly affects the uniformity of cell microenvironment, and further leads to the decline of cell activity, the fluctuation of proliferation efficiency and the uncontrollable differentiation result. Although the prior art has optimized the mixing operation, it still has a significant bottleneck in realizing high uniformity, and the specific problems can be summarized as follows: 1. The stirring degree is insufficient, and the mixing is not uniform enough. 2. Lack of filtration, and the stem cells are affected by flocculation.
[0003] Therefore, in order to solve the above technical problems, the stem cell mixing device is provided. CONTENT OF THE UTILITY MODEL
[0004] In view of the deficiencies of the prior art, the technical problem to be solved by the utility model is to provide a stem cell mixing device.
[0005] In order to solve the above technical problems, the technical scheme of the utility model is as follows: a stem cell mixing device, comprising a mixing pipeline, a first stirring assembly, a perforated baffle, a second stirring assembly, a cell screen and a third stirring assembly are sequentially arranged inside the mixing pipeline along the fluid flow direction.
[0006] Preferably, the mixing pipeline is composed of an inlet pipeline, a left intermediate pipeline, a right intermediate pipeline and an outlet pipeline which are sequentially connected along the fluid flow direction.
[0007] Preferably, the bottom end of the inlet pipeline is connected with the top end of the left intermediate pipeline, the bottom end of the left intermediate pipeline is connected with the bottom end of the right intermediate pipeline, and the top end of the right intermediate pipeline is connected with the bottom end of the outlet pipeline.
[0008] Preferably, the first stirring assembly, the perforated baffle, the second stirring assembly and the cell screen are vertically distributed along the axis of the left intermediate pipeline.
[0009] Preferably, the third stirring assembly is arranged at the joint of the connected bottom ends of the left and right intermediate pipelines.
[0010] Preferably, the bottom of the connected bottom ends of the left and right intermediate pipelines is a tapered structure which gradually narrows from top to bottom, and the third stirring assembly is located in the middle of the tapered structure.
[0011] Preferably, the bottom end of the tapered structure is a circular arc bottom.
[0012] Preferably, the first, second and third stirring assemblies each include a rotating shaft driven by a motor, and a plurality of radially extending stirring blades are circumferentially distributed on the rotating shaft.
[0013] Preferably, the perforated baffle plate is provided with a through-flow hole in the middle.
[0014] Preferably, the perforated baffle plate is in the shape of an arc structure with a low middle and a high periphery.
[0015] Compared with the prior art, the stem cell mixing device has the following beneficial effects: the stem cell mixing device is provided with three groups of stirring assemblies, namely, first, second and third stirring assemblies, the number of the stirring assemblies is large and the positions of the stirring assemblies are different, so that the stirring and mixing effect can be improved; the stem cell mixing device is provided with a cell screen, so that relatively large stem cells and flocculation can be filtered out.
[0016] The utility model will be further explained in detail in combination with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS
[0017] Figure 1 It is a structure schematic view of the embodiment of the utility model.
[0018] In the figure: first stirring assembly 1, perforated baffle plate 2, second stirring assembly 3, cell screen 4, third stirring assembly 5, inlet pipe 6, left middle pipe 7, right middle pipe 8, outlet pipe 9, conical structure 10, rotating shaft 11, stirring blade 12, through-flow hole 13. DETAILED DESCRIPTION
[0019] The utility model will be further explained in combination with the drawings and specific embodiments.
[0020] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
[0021] It should be noted that the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and furthermore, it should be understood that when the terms "comprise" and / or "include" are used in the specification, there is a presence of the features, steps, operations, devices, components and / or combinations thereof.
[0022] As shown in Figure 1 The embodiment provides a stem cell mixing device, which comprises a mixing pipeline, and the mixing pipeline is internally provided with a first stirring assembly 1, a perforated baffle plate 2, a second stirring assembly 3, a cell screen 4 and a third stirring assembly 5 in sequence along a fluid flow direction.
[0023] In the embodiment of the utility model, the mixing pipeline is composed of the inlet pipeline 6, the left intermediate pipeline 7, the right intermediate pipeline 8 and the outlet pipeline 9 which are sequentially communicated along the fluid flow direction.
[0024] In the embodiment of the utility model, the inlet pipeline bottom end is communicated with the left intermediate pipeline top end, the left intermediate pipeline bottom end is communicated with the right intermediate pipeline bottom end, and the right intermediate pipeline top end is communicated with the outlet pipeline bottom end.
[0025] In the embodiment of the utility model, the first stirring assembly, the perforated baffle, the second stirring assembly and the cell screen are vertically distributed along the axial direction of the left intermediate pipeline.
[0026] In the embodiment of the utility model, the third stirring assembly is arranged at the joint of the communicated bottom ends of the left and right intermediate pipelines.
[0027] In the embodiment of the utility model, the pipeline bottom of the communicated bottom ends of the left and right intermediate pipelines is a tapered structure 10 which gradually narrows from top to bottom, and the third stirring assembly is located at the middle part of the tapered structure.
[0028] In the embodiment of the utility model, the bottom tip of the tapered structure is a circular arc bottom.
[0029] In the embodiment of the utility model, the first, second and third stirring assemblies each include a rotating shaft 11 driven by a motor, and a plurality of radially extending stirring blades 12 are circumferentially distributed on the rotating shaft.
[0030] In the embodiment of the utility model, the perforated baffle is provided with a through-flow hole 13 in the middle.
[0031] In the embodiment of the utility model, the perforated baffle has an arc structure with a low middle and high periphery.
[0032] In the embodiment of the utility model, the pipeline wall of the left intermediate pipeline is provided with an inspection hole for facilitating the inspection or cleaning of the cell screen.
[0033] In the embodiment of the utility model, the working principle of the stem cell mixing device is as follows:
[0034] The stem cells exist in the form of particles suspended in a fluid in a specific experiment or engineering scenario, the inlet pipeline of the device is connected to a fluid source when in use, the fluid source is provided with flow power by a pump, and the outlet pipeline is connected to the next process.
[0035] Fluids from the inlet pipe into the left middle pipe, first through the first stirring assembly stirring, the hole baffle fluid slightly blocked, so that the fluid and the first stirring assembly contact stirring time extension;Fluids through the hole baffle in the middle of the flow hole down smoothly to the second stirring assembly, the second stirring assembly for the second time stirring, after the cell screen filter out the larger stem cells and flocculation, the fluid flows down to the tapered structure, the tapered structure to the fluid play a certain convergence function, the third stirring assembly for the third time centralized stirring, stirring fluid through the right middle pipe, outlet pipe to the next process.
[0036] The utility model are suitable for stem cell mixing operation product, the device features can be good and uniform mixing cell, avoid the influence of stem cell flocculation as far as possible;Stem cells and other culture medium reagent can be mixed uniformly.
[0037] The above is only the preferred embodiment of the utility model, and does not limit other forms of the utility model, and any skilled person in the art can change or modify the equivalent embodiment of equivalent change by using the disclosed technical content. However, any simple modification, equivalent change and modification of the above embodiment according to the technical essence of the utility model without departing from the technical scheme of the utility model still belongs to the protection scope of the technical scheme of the utility model.
Claims
1. A stem cell homogenizing device, characterized by: The mixing pipeline comprises a first stirring assembly, a perforated baffle, a second stirring assembly and a cell screen arranged in sequence along the fluid flow direction.
2. The stem cell mixing device of claim 1, wherein: The mixing pipeline comprises an inlet pipeline, a left intermediate pipeline, a right intermediate pipeline and an outlet pipeline which are connected in sequence along the fluid flow direction.
3. The stem cell mixing device of claim 2, wherein: The bottom end of the inlet pipeline is connected with the top end of the left intermediate pipeline, the bottom end of the left intermediate pipeline is connected with the bottom end of the right intermediate pipeline, and the top end of the right intermediate pipeline is connected with the bottom end of the outlet pipeline.
4. The stem cell mixing device of claim 3, wherein: The first stirring assembly, the perforated baffle, the second stirring assembly and the cell screen are vertically distributed along the axis of the left intermediate pipeline.
5. The stem cell mixing device of claim 3, wherein: The third stirring assembly is arranged at the joint of the connected bottom ends of the left and right intermediate pipelines.
6. The stem cell mixing device of claim 5, wherein: The bottom of the connected bottom ends of the left and right intermediate pipelines is a tapered structure which is gradually narrowed from top to bottom, and the third stirring assembly is located at the middle of the tapered structure.
7. The stem cell mixing device of claim 6, wherein: The bottom tip of the tapered structure is a circular arc bottom.
8. The stem cell mixing device of claim 1, wherein: The first, second and third stirring assemblies each comprise a rotating shaft driven by a motor, and a plurality of radially extending stirring blades are circumferentially distributed on the rotating shaft.
9. The stem cell mixing device of claim 1, wherein: The perforated baffle is provided with a through-flow hole in the middle.
10. The stem cell mixing device of claim 1, wherein: The perforated baffle has an arc structure with a low middle and high periphery.