Multi-connected test paper

Abstract

The utility model relates to detection test paper, in particular to multi-union detection test paper which is characterized by comprising a shell, a filter layer and test paper, a sample inlet and an observation opening are formed in the shell at intervals, the test paper is arranged in the shell and provided with a feeding area and a detection area, the detection area is provided with a plurality of sub-detection areas, and the sub-detection areas are communicated with the filter layer. The test paper feeding area is positioned below the sample inlet; the plurality of sub-detection areas are arranged along the length direction of the test paper, each sub-detection area is used for detecting different target substances in a sample, and the detection area is positioned in the observation opening; the filter layer is arranged between the test paper feeding area and the sample inlet, the sample inlet is suitable for placing a sample, the sample can enter the test paper through the sample inlet and the filter layer, and the filter layer is used for filtering impurities in the sample. The method has the advantage of improving the detection efficiency.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of test paper, and relates to a multi-association test paper. BACKGROUND

[0002] In the field of modern biotechnology and medicine, multi-enzyme cleaning solution is widely used in the cleaning process of laboratory instruments, medical devices and industrial equipment. These cleaning solutions usually contain multiple enzymes such as protease, lipase, amylase and cellulase, each of which has a specific degradation effect on different types of stains.

[0003] However, the commercially available test paper usually only detects a certain enzyme (such as protease), and cannot comprehensively evaluate the overall activity of the multi-enzyme cleaning solution. The limitation of this single enzyme detection method is that it can only reflect the activity level of a certain enzyme in the cleaning solution, and cannot provide information about the activity of other enzymes. The existing single enzyme detection method has obvious limitations and cannot meet the demand for overall activity evaluation of multi-enzyme cleaning solution. SUMMARY

[0004] The technical problem to be solved by the present application is to provide a multi-association test paper to simultaneously detect the activity of multiple enzymes in a multi-enzyme cleaning solution.

[0005] To solve the above technical problems, the present application provides a multi-association test paper, comprising a shell, a filter layer and a test paper,

[0006] The shell is provided with a sample inlet and an observation port spaced apart from each other, the test paper is arranged in the shell, the test paper has a feeding area and a detection area, the detection area has a plurality of sub-detection areas, and the feeding area of the test paper is located below the sample inlet;

[0007] A plurality of sub-detection areas are arranged along the length direction of the test paper, each of the sub-detection areas is used for detecting different target substances in the sample, and the detection area is located in the observation port;

[0008] The filter layer is arranged between the feeding area of the test paper and the sample inlet, the sample inlet is adapted to put in a sample, the sample can enter the test paper through the sample inlet and the filter layer, and the filter layer is used for filtering impurities in the sample.

[0009] Optionally, a plurality of gradient color developing cards are arranged on the outer surface of the shell, each of the gradient color developing cards corresponds to one of the detection areas and is arranged on one side of the corresponding detection area along the width direction of the test paper.

[0010] Optionally, a plurality of grooves are arranged on the outer surface of the shell, each of the gradient color developing cards is arranged in one of the grooves, and the gradient color developing card is bonded in the groove.

[0011] Optionally, at least one side of the observation port along the width direction of the test paper is provided with a plurality of gradient color developing cards, and adjacent gradient color developing cards on the same side of the observation port are arranged at intervals along the length direction of the test paper.

[0012] Optionally, the sample inlet is in the shape of a tapered hole, the tip of the sample inlet is close to the sample inlet end of the test paper, and the filter layer is fixed at the sample inlet end of the test paper.

[0013] Optionally, the test paper further comprises a water absorption layer, the water absorption layer is located at one end of the test paper away from the sample inlet end, and the water absorption layer is respectively bonded to the shell and the test paper.

[0014] Optionally, the shell comprises an upper shell and a lower shell, the sample inlet and the observation port are arranged on the upper shell, the upper shell and the lower shell are detachably connected, and the test paper is fixed on the lower shell.

[0015] Optionally, the observation port is provided with a transparent plate, and the transparent plate seals the observation port.

[0016] The multi-association detection test paper provided in the embodiment of the application has three sub-detection zones, one of which is used for protease detection, and the chemical reaction of azocasein and protease occurs color developing reaction: the color changes from yellow to red. Another is for lipase detection, and the color developing reaction occurs between bromothymol blue-olive oil emulsion and lipase, and the color changes from blue to green. The third sub-detection zone is for amylase detection, and the color developing reaction occurs between iodine-starch complex and amylase, and the color changes from blue to colorless. The three enzymes can be detected at the same time, and the detection efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 is the overall schematic diagram of the multi-association detection test paper provided in the application;

[0018] Figure 2 is the test paper structure diagram of the multi-association detection test paper provided in the application.

[0019] The reference signs in the specification are as follows:

[0020] 1, shell; 11, upper shell; 12, lower shell; 13, sample inlet; 14, observation port; 2, test paper; 21, sub-detection zone; 3, filter layer; 4, gradient color developing card; 5, water absorption layer; 6, transparent plate. DETAILED DESCRIPTION

[0021] In order to make the technical problems, technical solutions and beneficial effects solved by the present application clearer, the present application will be further described in detail below in conjunction with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and not intended to limit the present application.

[0022] Referring to Figure 1 and Figure 2 The present application provides a multi-association test paper 2, comprising a shell 1, a filter layer 3 and a test paper 2,

[0023] The shell 1 is provided with a sample inlet 13 and an observation port 14 spaced from each other, the test paper 2 is arranged in the shell 1, the test paper 2 has a feeding area and a detection area, the detection area has a plurality of sub-detection areas 21, and the feeding area of the test paper 2 is located below the sample inlet 13;

[0024] A plurality of sub-detection areas 21 are arranged along the length direction of the test paper 2, each of the sub-detection areas 21 is used for detecting different target substances in the sample, and the detection area is located in the observation port 14;

[0025] The filter layer 3 is arranged between the feeding area of the test paper 2 and the sample inlet 13, the sample inlet 13 is adapted to put in a sample, the sample can enter the test paper 2 through the sample inlet 13 and the filter layer 3, and the filter layer 3 is used for filtering impurities in the sample.

[0026] In the present application, the sub-detection areas 21 are three, one of which is used for protease detection, and the protease chemical reaction with azocasein occurs color reaction: the color changes from yellow to red. Another is lipase detection, and the bromothymol blue-olive oil emulsion and lipase occur color reaction, and the color changes from blue to green. The third sub-detection area 21 is amylase detection, and the iodine-starch complex and amylase occur color reaction, and the color changes from blue to colorless.

[0027] When the sample passes through the filter layer 3 from the sample inlet 13 and gradually penetrates to the detection area, the sample passes through the three sub-detection areas 21 one by one, and the protease, lipase and amylase in the sample react with different color substrates in the sub-detection areas 21 respectively, so as to determine whether the multiple enzymes in the sample exist.

[0028] As an example, the multi-association inspection test paper 2 further comprises a plurality of gradient color developing cards 4 arranged on the outer surface of the shell 1, each of the gradient color developing cards 4 corresponds to one of the detection zones and is arranged on one side of the corresponding detection zone along the width direction of the test paper 2. By designing the substrate concentration gradient, semi-quantitative detection of different enzyme concentrations is realized, and semi-quantitative comparison (for example, “insufficient / qualified / excessive”) is realized by color comparison. A single detection can simultaneously obtain multiple enzyme concentration data, and the efficiency is improved by more than 3 times.

[0029] As an example, a plurality of grooves are arranged on the outer surface of the shell 1, each of the gradient color developing cards 4 is placed in one of the grooves, and the gradient color developing card 4 is bonded in the groove. In this embodiment, when the gradient color developing card 4 is installed, glue is squeezed into the groove in advance, and then the gradient color developing card 4 is placed in the groove, so that the gradient color developing card 4 is stably bonded in the groove, and the groove also limits the position of the gradient color developing card 4, facilitating assembly.

[0030] As an example, at least one of the two opposite sides of the observation port 14 along the width direction of the test paper 2 is provided with a plurality of gradient color developing cards 4, and adjacent gradient color developing cards 4 located on the same side of the observation port 14 are arranged at intervals along the length direction of the test paper 2.

[0031] In order to facilitate observation and avoid confusion between the gradient color developing card 4 and the comparison results of the color developing reaction, each gradient color developing card 4 is arranged at intervals with adjacent gradient color developing cards 4 to avoid confusion in visual comparison.

[0032] As an example, the sample inlet 13 is in the shape of a tapered hole, the tip of the sample inlet 13 is close to the sample inlet end of the test paper 2, and the filter layer 3 is fixed on the sample inlet end of the test paper 2. The upper surface of the filter layer 3 closes the sample inlet 13, and the lower surface of the filter layer 3 is fixed on the sample inlet end of the test paper 2.

[0033] As an example, the multi-association inspection test paper 2 further comprises a water absorption layer 5, which is located at one end of the test paper 2 away from the sample inlet end, and the water absorption layer 5 is bonded with the shell 1 and the test paper 2 respectively. If the sample amount of the user is too large, to avoid too much liquid sample from spilling out of the test paper 2 and flowing into the shell 1, the sample in the shell 1 may flow out due to shaking of the multi-association inspection test paper 2, to avoid such a situation, a water absorption layer 5 is added at the end of the test paper 2 away from the sample inlet end to store excess sample in the water absorption layer 5.

[0034] As an example, the housing 1 comprises an upper shell 11 and a lower shell 12, the sample inlet 13 and the observation port 14 are arranged on the upper shell 11, and the upper shell 11 and the lower shell 12 are detachably connected, generally through buckling to achieve the detachable connection of the two, and the test paper 2 is fixed on the lower shell 12.

[0035] As an example, the observation port 14 is built-in with a transparent plate 6, Figure 1 The transparent plate 6 is indicated by a dashed line, and the transparent plate 6 closes the observation port 14. In this embodiment, the transparent plate 6 made of plastic material is used to close the observation port 14, so as to avoid the pollution of external substances to the test paper 2 and improve the detection effect.

[0036] The above is only a preferred embodiment of the present application, and is not used to limit the present application, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A multi-parallel detection test paper, characterized in that, The device comprises a shell, a filter layer and a test paper, The shell is provided with a sample inlet and an observation window at intervals, the test paper is arranged in the shell, the test paper has a feeding area and a detection area, the detection area has a plurality of sub-detection areas, and the feeding area of the test paper is located below the sample inlet; A plurality of sub-detection areas are arranged along the length direction of the test paper, each of the sub-detection areas is used for detecting different target substances in the sample, and the detection area is located in the observation window; The filter layer is arranged between the feeding area of the test paper and the sample inlet, the sample inlet is adapted to put in a sample, the sample can enter the test paper through the sample inlet and the filter layer, and the filter layer is used for filtering impurities in the sample.

2. The multiplex test paper according to claim 1, characterized in that, A plurality of gradient color developing cards are arranged on the outer surface of the shell, each of the gradient color developing cards corresponds to one of the detection areas and is arranged on one side of the corresponding detection area along the width direction of the test paper.

3. The multiplex test paper according to claim 2, characterized in that, The outer surface of the shell is provided with a plurality of grooves, each of the gradient color developing cards is arranged in one of the grooves, and the gradient color developing card is bonded in the groove.

4. The multiplex test paper according to claim 2, characterized in that, At least one of the two opposite sides of the observation window along the width direction of the test paper is provided with a plurality of gradient color developing cards, and adjacent gradient color developing cards located on the same side of the observation window are arranged at intervals along the length direction of the test paper.

5. The multiplex test paper according to claim 4, characterized in that, The sample inlet is in the shape of a tapered hole, the tip of the sample inlet is close to the sample inlet end of the test paper, and the filter layer is fixed at the sample inlet end of the test paper.

6. The multiplex test paper according to claim 5, characterized in that, A water absorption layer is further included, the water absorption layer is located at one end of the test paper away from the sample inlet end, and the water absorption layer is bonded with the shell and the test paper respectively.

7. The multiplex test paper according to claim 4, wherein The shell comprises an upper shell and a lower shell, the sample inlet and the observation window are arranged on the upper shell, the upper shell and the lower shell are detachably connected, and the test paper is fixed on the lower shell.

8. The multi-project test paper according to claim 1, characterized in that, The observation window is provided with a transparent plate, and the transparent plate seals the observation window.

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