Cell culture bed for bioreactor

Abstract

The utility model discloses a cell culture bed for a bioreactor, and relates to the technical field of cell culture. Comprising a housing, a first bearing seat, a rotating shaft, a first gear, a first motor, a second gear, a circular groove, a pulley, a rotating disc, a second bearing seat, a sliding rail, a second motor, a lead screw, a sliding block, a mounting plate, an arc-shaped groove, a spherical pin shaft, an illuminating lamp, a support, a locking rod, a pneumatic cylinder, a culture solution adding chamber, a valve, an adding nozzle, a feeding opening, a clamping device and the like. The culture solution adding chamber is driven by the pneumatic cylinder, and the culture solution can be accurately added according to cell growth requirements by matching with the valve and the adding nozzle. When it is observed that cell growth enters a specific stage and needs more nutrition, the culture solution can be added in time, insufficient or excessive cell nutrition supply is avoided, a stable and appropriate growth environment is created for cells, and the quality and efficiency of cell culture are effectively improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, especially a cell culture bed for bioreactor. BACKGROUND

[0002] In the field of cell culture technology, the traditional cell culture equipment has many drawbacks, which adversely affect the quality, efficiency and operation convenience of cell culture.

[0003] Culture environment control: The existing equipment often cannot add culture solution in time according to the real-time growth state of cells, leading to insufficient or excessive nutrition supply during cell growth, limiting the improvement of cell growth efficiency and quality.

[0004] Cell observation and operation convenience: The existing cell culture equipment has great defects in cell observation. The lighting device position is fixed and the angle is not adjustable, which is difficult to meet the needs of clear observation of cells from different angles. Researchers cannot observe the characteristics of cell morphology and growth state comprehensively, which is not conducive to timely discovery of abnormal changes and potential problems of cells.

[0005] Cell fixation and protection: The stability of the traditional cell culture device in fixing the cell culture container is poor. During the operation of the equipment, the culture container is easy to shake, which not only destroys the growth environment of cells, but also may cause the collision and damage of the cell culture container, resulting in the failure of cell culture. At the same time, the fixing structure lacks buffer protection measures, which cannot effectively reduce the impact on the cell culture container when the outside is shaken or the equipment is moved, further threatening the survival and growth of cells. UTILITY MODEL CONTENT

[0006] The utility model aims at solving the above problems and provides a cell culture bed for bioreactor.

[0007] To solve the above problems, the utility model provides a technical scheme: a cell culture bed for bioreactor, including cover, first bearing seat, pivot, first gear, first motor, second gear, round groove, pulley, carousel, second bearing seat, slide rail, second motor, lead screw, sliding block, mounting plate, arc slot, spherical pin shaft, illuminating lamp, support, locking rod, pneumatic cylinder, culture solution adding chamber, valve, adding mouth, feed inlet and holder, the first bearing seat and first motor are fixedly connected on the inside bottom surface center of cover chamber, the pivot is movably connected in the first bearing seat, the carousel is fixedly connected on the top end of pivot, a plurality of holders are arranged on carousel in the form of circular array, the first gear is fixedly connected on the middle end position of pivot, the second gear is fixedly connected on the output end of first motor, the first gear and second gear are engaged together, the pulley is connected on both sides of carousel lower surface and is slidably connected in the round groove of the inside bottom surface of cover chamber, the pneumatic cylinder is fixedly connected on the right side of cover chamber top end, the culture solution adding chamber is fixedly connected on the output end of pneumatic cylinder, the feed inlet is arranged on both sides of culture solution adding chamber top end, the adding mouth is communicated with culture solution adding chamber bottom end through valve, the adding mouth is aligned with holder, the slide rail is fixedly connected on the left side chamber wall of cover, the second bearing seat is fixedly connected on the left side top end of cover chamber, the second motor is fixedly connected on the left side bottom end of cover chamber, the lead screw is fixedly connected on the output end of second motor, the sliding block is movably connected on the lead screw top end in second bearing seat, the sliding block is slidably connected on the slide rail left side, the mounting plate is fixedly connected on the sliding block right side, the arc slot is fixedly connected on the mounting plate right side, the spherical pin shaft is rotatably connected in the arc slot, the illuminating lamp is fixedly connected on the spherical pin shaft right side, the support is fixedly connected on the arc slot bottom surface, the locking rod is threadedly connected in the support, and the top end of locking rod abuts against spherical pin shaft.

[0008] As preferred, the specific structure of the holder comprises holder cover, fixed clamping shell, pin shaft, limiting block, buffer pad, pull rod, spring and handle, the holder cover is fixedly connected on the carousel, the fixed clamping shell is arranged on both sides of the chamber inside of holder cover, the buffer pad is arranged on the inner side of fixed clamping shell, the spring is fixedly connected between fixed clamping shell and holder cover, the pin shaft is slidably connected in holder cover and is fixedly connected on the lower end of the outer side of fixed clamping shell, the limiting block is fixedly connected on the outer side end of pin shaft, the pull rod is fixedly connected on the upper end of the outer side of fixed clamping shell, and the handle is fixedly connected on the outer side end of pull rod.

[0009] As preferred, the handle is provided with anti-skid lines.

[0010] As preferred, the spring is coated with wear-resistant coating.

[0011] As preferred, the slide rail is coated with lubricating grease.

[0012] As preferred, the side of the housing is hinged with a hatch by a hinge.

[0013] The beneficial effects of the utility model are: (1) precise regulation and control of culture environment: the air cylinder drives the culture solution adding chamber, cooperates with the valve and the adding nozzle, and can accurately add culture solution according to the cell growth demand. When the cell growth is observed to enter a specific stage and more nutrition is needed, the culture solution can be added in time to avoid insufficient or excessive cell nutrition supply, create a stable and suitable growth environment for cells, and effectively improve the quality and efficiency of cell culture.

[0014] (2) optimization of cell observation experience: the second motor, lead screw, sliding block, arc-shaped groove, spherical pin shaft and locking rod and other components work cooperatively, so that the illuminating lamp can be flexibly adjusted in position and angle. The scientific researcher can illuminate the cell culture container from different directions to observe the cell morphology, growth state and other characteristics comprehensively and clearly. For example, when observing the cell division process, the light angle can be adjusted at any time to capture key details and discover abnormal changes and potential problems of cells in time to provide accurate basis for scientific research.

[0015] (3) stable fixation of cell culture container: the special structure design of the clamp includes the fixed clamping shell, spring and buffer pad, which not only can stably fix the cell culture container to prevent it from shaking during the operation of the equipment, but also has good buffer protection performance. When the equipment is subjected to external vibration or movement, the spring and buffer pad can effectively reduce the impact on the culture container, reduce the risk of collision and damage of the cell culture container, protect the survival and growth environment of cells, and improve the success rate of cell culture.

[0016] (4) overall performance advantage is prominent: the overall structure of the cell culture bed is reasonable and simple, and the components cooperate closely with each other, so that the production cost is effectively controlled. Moreover, the installation process is relatively simple, which reduces the installation difficulty and time cost. In addition, the equipment integrates culture solution adding, cell observation and container fixation and other functions, is complete in function, and can meet the diversified needs in the cell culture process to provide an efficient and convenient experimental tool for scientific researchers. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a structure schematic view of the utility model.

[0018] Figure 2 It is a local enlarged structure schematic view of the utility model Figure One .

[0019] Figure 3 It is a local enlarged structure schematic view of the utility modelFigure Two .

[0020] Figure 4 The partial enlarged structure diagram of the utility model Figure Three .

[0021] 1 - cover; 2 - first bearing seat; 3 - rotating shaft; 4 - first gear; 5 - first motor; 6 - second gear; 7 - round groove; 8 - pulley; 9 - rotating disc; 10 - second bearing seat; 11 - slide rail; 12 - second motor; 13 - lead screw; 14 - sliding block; 15 - mounting plate; 16 - arc slot; 17 - spherical pin shaft; 18 - illuminating lamp; 19 - support; 20 - locking rod; 21 - pneumatic cylinder; 22 - culture solution adding chamber; 23 - valve; 24 - adding nozzle; 25 - feed inlet; 26 - gripper cover; 27 - fixed clamping shell; 28 - pin shaft; 29 - limiting block; 30 - buffer pad; 31 - pull rod; 32 - spring; 33 - handle. DETAILED DESCRIPTION

[0022] As Figures 1 to 4As shown in the specific embodiment, the following technical solutions are adopted: a cell culture bed for a bioreactor, comprising a cover 1, a first bearing seat 2, a rotating shaft 3, a first gear 4, a first motor 5, a second gear 6, a circular groove 7, a pulley 8, a rotating disc 9, a second bearing seat 10, a sliding rail 11, a second motor 12, a lead screw 13, a sliding block 14, a mounting plate 15, an arc-shaped groove 16, a spherical pin shaft 17, an illuminating lamp 18, a support 19, a locking rod 20, a pneumatic cylinder 21, a culture solution adding chamber 22, a valve 23, an adding nozzle 24, a feeding port 25, and a holder; the first bearing seat 2 and the first motor 5 are fixedly connected to the center of the bottom surface inside the cavity of the cover 1; the rotating shaft 3 is movably connected in the first bearing seat 2, the rotating disc 9 is fixedly connected to the top end of the rotating shaft 3, and a plurality of holders are arranged on the rotating disc 9 in a circular array; the first gear 4 is fixedly connected to the middle end position of the rotating shaft 3, the second gear 6 is fixedly connected to the output end of the first motor 5, and the first gear 4 and the second gear 6 are engaged with each other; the pulley 8 is connected to the lower surface of the rotating disc 9 on both sides and is slidingly connected in the circular groove 7 prearranged in the bottom surface inside the cavity of the cover 1; the pneumatic cylinder 21 is fixedly connected to the right top end inside the cavity of the cover 1, the culture solution adding chamber 22 is fixedly connected to the output end of the pneumatic cylinder 21, the feeding port 25 is arranged on both sides of the top end of the culture solution adding chamber 22, the adding nozzle 24 is communicated with the culture solution adding chamber 22 through the valve 23 at the bottom end of the culture solution adding chamber 22, and the adding nozzle 24 is aligned with the holder; the sliding rail 11 is fixedly connected to the left cavity wall of the cover 1; the second bearing seat 10 is fixedly connected to the left top end inside the cavity of the cover 1, the second motor 12 is fixedly connected to the left bottom end inside the cavity of the cover 1, the lead screw 13 is fixedly connected to the output end of the second motor 12, and the top end of the lead screw 13 is movably connected in the second bearing seat 10; the sliding block 14 is threadedly connected to the lead screw 13, the left side of the sliding block 14 is slidingly connected to the sliding rail 11, the right side of the sliding block 14 is fixedly connected to the mounting plate 15, the right side of the mounting plate 15 is fixedly connected to the arc-shaped groove 16, the spherical pin shaft 17 is rotatably connected in the arc-shaped groove 16, and the illuminating lamp 18 is fixedly connected to the right side of the spherical pin shaft 17; the support 19 is fixedly connected to the bottom surface of the arc-shaped groove 16, the locking rod 20 is threadedly connected in the support 19, and the top end of the locking rod 20 abuts against the spherical pin shaft 17.

[0023] As preferred, the specific structure of the holder comprises a holder cover 26, a fixed clamping shell 27, a pin shaft 28, a limiting block 29, a buffer pad 30, a pull rod 31, a spring 32 and a handle 33; the holder cover 26 is fixedly connected on the rotating disc 9; both sides of the cavity of the holder cover 26 are provided with the fixed clamping shell 27, and the inner side of the fixed clamping shell 27 is provided with the buffer pad 30; the spring 32 is fixedly connected between the fixed clamping shell 27 and the holder cover 26; the lower end of the outer side of the fixed clamping shell 27 is fixedly connected with the pin shaft 28, the pin shaft 28 is slidingly connected in the holder cover 26, and the outer side end of the pin shaft 28 is fixedly connected with the limiting block 29; the upper end of the outer side of the fixed clamping shell 27 is fixedly connected with the pull rod 31, and the outer side end of the pull rod 31 is fixedly connected with the handle 33.

[0024] Wherein, the handle 33 is provided with anti-skid lines; the spring 32 is coated with a wear-resistant coating; the sliding rail 11 is coated with lubricating grease; and the side of the cover 1 is hingedly connected with a door through a hinge.

[0025] The use state of the utility model is: (1) the placing and fixing of the cell culture container: opening the door of the cover 1 through the hinge, the researcher holds the holder handle 33 and pulls the pull rod 31, driving the fixed clamping shell 27 to move outward against the elastic force of the spring 32. At this time, the cell culture container is placed in the holder cover 26, the handle 33 is loosened, and under the action of the spring 32, the fixed clamping shell 27 moves inward, and the buffer pad 30 on the inner side is tightly attached to the cell culture container, achieving stable fixing and preventing the culture container from shaking during the operation of the equipment. At the same time, the pin shaft 28 slides in the holder cover 26, and the limiting block 29 plays a role in limiting the moving range of the fixed clamping shell 27.

[0026] (2) adding of the culture solution: when the culture solution needs to be added, the pneumatic cylinder 21 is started, the output end pushes the culture solution adding chamber 22 to move downward, and the adding nozzle 24 is close to the cell culture container. Then, the valve 23 is opened, the culture solution in the culture solution adding chamber 22 is injected from the feed inlet 25 in advance, and flows into the cell culture container through the adding nozzle 24. The researcher can accurately control the opening and closing degree of the valve 23 and the extension amount of the pneumatic cylinder 21 according to the cell growth state, so as to realize the accurate addition of the culture solution and provide a suitable nutrient environment for the cells.

[0027] (3) Cell observation: if you want to observe the cells, start the second motor 12, the output end drives the screw rod 13 to rotate. The slider 14 on the screw rod 13 moves up and down under the guidance of the slide rail 11, and in turn drives the mounting plate 15, the arc-shaped groove 16 and the illuminating lamp 18 to move up and down, so as to adjust the lighting height. When it is necessary to adjust the lighting angle, loosen the locking rod 20 connected by threads in the support 19, so that the spherical pin shaft 17 can rotate in the arc-shaped groove 16, and after the illuminating lamp 18 is rotated to the appropriate angle, tighten the locking rod 20 against the spherical pin shaft 17 to fix the lighting angle. At this time, the researchers can clearly observe the characteristics of the cells in the cell culture container from different angles, such as the morphology, growth state and the like, and timely find the abnormal changes of the cells.

[0028] (4) Overall operation of the cell culture bed: during the cell culture process, the first motor 5 is started, and the second gear 6 at the output end drives the first gear 4 on the rotating shaft 3 to rotate, and in turn drives the rotating shaft 3 to rotate, and the rotating disc 9 fixed at the top end of the rotating shaft 3 rotates. The pulleys 8 on both sides of the lower surface of the rotating disc 9 slide in the circular groove 7 pre-set on the inner bottom surface of the housing 1, so as to ensure the stable rotation of the rotating disc 9. A plurality of cell culture containers on the rotating disc 9 rotate, so that the adding nozzle 24 can be aligned with the cell culture container in the holder when the culture solution is added in the above step, and at the same time, the cell observation in the above step is facilitated, so that the illuminating lamp 18 can be effectively aligned with the cell culture container in the holder, and the observation is facilitated.

[0029] In the description of the utility model, it is to be understood that the orientation or positional relationship indicated by the terms "coaxial", "bottom", "one end", "top", "middle", "the other end", "upper", "one side", "top", "inner", "front", "central", "both ends" and the like is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as a limitation on the utility model.

[0030] In the utility model, unless otherwise explicitly specified and limited, the terms "mounting", "setting", "connecting", "fixing", "threading" and the like should be understood in a broad sense, for example, can be fixedly connected, or can be detachably connected, or can be integrated; can be mechanically connected, or can be electrically connected; can be directly connected, or can be indirectly connected through an intermediate medium; can be the communication or interaction relationship between two elements, unless otherwise explicitly limited, and the specific meaning of the above terms in the utility model can be understood according to the specific circumstances by the ordinary skilled person in the art.

[0031] The basic principle and main features of the utility model and the advantages of the utility model are shown and described above, and the person skilled in the art should understand that the utility model is not limited by the above examples, and the above examples and the description in the specification are only to illustrate the principle of the utility model, and various changes and improvements can be made to the utility model without departing from the spirit and scope of the utility model, and these changes and improvements all fall within the scope of the utility model claimed, and the scope of protection of the utility model is defined by the appended claims and equivalents thereof.

[0032] The control mode of the utility model is controlled by manually starting and closing the switch, and the wiring diagram of the power element and the provision of the power supply are common knowledge in the art, and the utility model is mainly used for protecting the mechanical device, so the control mode and the wiring arrangement of the utility model will not be explained in detail.

Claims

1. A cell culture bed for a bioreactor, characterized by: It includes cover (1), first bearing seat (2), shaft (3), first gear (4), first motor (5), second gear (6), round groove (7), pulley (8), rotating disc (9), second bearing seat (10), slide rail (11), second motor (12), lead screw (13), sliding block (14), mounting plate (15), arc slot (16), spherical pin shaft (17), illuminating lamp (18), support (19), locking rod (20), pneumatic cylinder (21), culture solution adding chamber (22), valve (23), adding nozzle (24), feed inlet (25) and holder; The first bearing seat (2) and the first motor (5) are fixedly connected to the center of the bottom surface inside the chamber of the cover (1); The first bearing seat (2) is movably connected with the shaft (3), and the top end of the shaft (3) is fixedly connected with the rotating disc (9), and a plurality of holders are arranged on the rotating disc (9) in a circular array; The first gear (4) is fixedly connected to the middle end position of the shaft (3), and the output end of the first motor (5) is fixedly connected with the second gear (6), and the first gear (4) and the second gear (6) are engaged with each other; The lower surface of the rotating disc (9) is connected with the pulley (8) on both sides, and the pulley (8) is slidably connected in the round groove (7) prearranged in the bottom surface inside the chamber of the cover (1); The top right side of the chamber inside the cover (1) is fixedly connected with the pneumatic cylinder (21), the output end of the pneumatic cylinder (21) is fixedly connected with the culture solution adding chamber (22), the top of the culture solution adding chamber (22) is provided with the feed inlet (25), the bottom of the culture solution adding chamber (22) is communicated with the adding nozzle (24) through the valve (23), and the adding nozzle (24) is aligned with the holder; The left side chamber wall of the cover (1) is fixedly connected with the slide rail (11); The left side top of the chamber inside the cover (1) is fixedly connected with the second bearing seat (10), and the left side bottom of the chamber inside the cover (1) is fixedly connected with the second motor (12), and the output end of the second motor (12) is fixedly connected with the lead screw (13), and the top end of the lead screw (13) is movably connected in the second bearing seat (10); The lead screw (13) is threadedly connected with the sliding block (14), the left side of the sliding block (14) is slidably connected in the slide rail (11), the right side of the sliding block (14) is fixedly connected with the mounting plate (15), the right side of the mounting plate (15) is fixedly connected with the arc slot (16), the arc slot (16) is rotatably connected with the spherical pin shaft (17), and the right side of the spherical pin shaft (17) is fixedly connected with the illuminating lamp (18); The bottom surface of the arc slot (16) is fixedly connected with the support (19), the support (19) is threadedly connected with the locking rod (20), and the top end of the locking rod (20) abuts against the spherical pin shaft (17).

2. The cell culture bed for a bioreactor of claim 1, wherein: The specific structure of the holder includes holder cover (26), fixed clamping shell (27), pin shaft (28), limiting block (29), buffer pad (30), pull rod (31), spring (32) and handle (33). The clamping cover (26) is fixedly connected on the rotary table (9); Both sides of the chamber of the clamping cover (26) are provided with fixed clamping shells (27), and the inner sides of the fixed clamping shells (27) are provided with buffer pads (30); The fixed clamping shells (27) and the clamping cover (26) are fixedly connected with springs (32); The lower ends of the outer sides of the fixed clamping shells (27) are fixedly connected with pin shafts (28), the pin shafts (28) are slidingly connected in the clamping cover (26), and the outer side ends of the pin shafts (28) are fixedly connected with limit blocks (29); The upper ends of the outer sides of the fixed clamping shells (27) are fixedly connected with pull rods (31), and the outer side ends of the pull rods (31) are fixedly connected with handles (33).

3. The cell culture bed for a bioreactor of claim 2, wherein: Anti-skid lines are arranged on the handles (33).

4. The cell culture bed for a bioreactor of claim 2, wherein: The springs (32) are coated with wear-resistant coatings.

5. The cell culture bed for a bioreactor of claim 1, wherein: The slide rails (11) are coated with lubricating grease.

6. The cell culture bed for a bioreactor of claim 1, wherein: A warehouse door is hingedly connected to the side of the cover (1) through a hinge.

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