Integrated fluorescence reaction device

Abstract

The utility model relates to the technical field of fluorescence detection, and discloses an integrated fluorescence reaction device which comprises a detection box, equipment for fluorescence detection is arranged in the detection box, a horizontal sliding plate is arranged in the detection box in a sliding manner, and a placing plate for placing a test tube is arranged at the top of the sliding plate in a sliding manner; a transmission mechanism is mounted between the bottom of the placement plate and the detection box, the placement plate is in transmission fit with the sliding plate through the transmission mechanism, and the transmission mechanism is used for driving the placement plate to reciprocate in the horizontal direction; the detection box is provided with a box opening for the sliding plate and the placing plate to slide out, and a sealing box door is installed at the box opening and fixedly connected with the sliding plate. An electric push rod is fixedly mounted on the detection box; an output shaft of the electric push rod is fixedly connected with the sealing box door; a controller is fixedly installed on the detection box, and the electric push rod is electrically connected with the controller. The controller is simple in structure, low in control difficulty, low in power consumption and low in detection cost.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of fluorescence detection technology, especially to a one-piece fluorescence reaction device. BACKGROUND

[0002] Fluorescence reaction refers to the process in which certain substances, after absorbing light energy, are in an excited state, and then release energy in the form of light when returning to the ground state from the excited state. These substances emit visible light when irradiated by specific wavelengths of light. This technology is mainly applied in the fields of anti-counterfeiting, biomedicine and material science. In the field of biomedicine, fluorescent dyes can be used to label biomolecules such as proteins and nucleic acids. Under a fluorescence microscope, the location, distribution and dynamic changes of these labeled molecules in cells can be observed, which helps to study the structure and function of cells.

[0003] The food fluorescence detection device disclosed in the utility model has the advantages that the food fluorescence detection device is provided with the sealing cover plate, the sample storage base frame, the test tube storage rack, the test tube insertion port, the bottom positioning groove and the guide buffer column, so that the food fluorescence detection device can be used for the detection of food, and the sealing cover plate can be used for sealing the food in the food fluorescence detection device, and the sample storage base frame and the test tube storage rack can be used for storing the sample and the test tube, and the test tube insertion port and the bottom positioning groove can be used for guiding the test tube to be inserted into the test tube insertion port and to be positioned in the bottom positioning groove, and the guide buffer column can be used for buffering the test tube when the test tube is inserted into the test tube insertion port, so that the test tube can be prevented from being damaged.

[0004] Based on the above technical features, the problem is that in the prior art, the reciprocating sliding of the test tube rack is driven by a single motor, and another motor is additionally used to drive the sealing cover plate to slide, resulting in increased power consumption of the detection device, increased detection cost, and increased control difficulty of the control unit for the two motors.

[0005] Therefore, it is necessary to solve the above problems by using a one-piece fluorescence reaction device. UTILITY MODEL CONTENTS

[0006] The utility model aims at providing a one-piece fluorescence reaction device to solve the problems in the background art.

[0007] In order to achieve the above object, the utility model provides the following technical scheme: a device of integral fluorescent reaction, including detection box, the equipment for fluorescent detection is arranged in the detection box, the horizontal slide plate is installed in the detection box, the top of the slide plate is slidably installed for placing the test tube's placing plate;

[0008] The transmission mechanism is installed between the bottom of the placing plate and the detection box, the placing plate is drivenly matched with the slide plate through the transmission mechanism, and the transmission mechanism is used for driving the placing plate to reciprocatingly slide in the horizontal direction;

[0009] The box mouth is installed on the detection box, the box mouth is used for the slide of the slide plate and the placing plate, the sealing box door is installed at the box mouth, and the sealing box door is fixedly connected with the slide plate;

[0010] The electric push rod is fixedly installed on the detection box, and the output shaft of the electric push rod is fixedly connected with the sealing box door;

[0011] The controller is fixedly installed on the detection box, and the electric push rod is electrically connected with the controller.

[0012] Preferably, the transmission mechanism includes a vertical transmission shaft, the bottom of the slide plate is fixedly installed with a vertical support shaft, a connecting rod is fixedly arranged on the support shaft in the radial direction, the transmission shaft penetrates the end of the connecting rod away from the support shaft and is rotationally connected with the connecting rod, the transmission shaft is drivenly matched with the slide plate through a first transmission assembly, the first transmission assembly is used for driving the transmission shaft to rotate, and the placing plate is drivenly matched with the transmission shaft through a second transmission assembly, and the second transmission assembly is used for driving the placing plate to reciprocatingly slide in the horizontal direction.

[0013] Preferably, the first transmission assembly includes a first gear, a second gear and a rack, the rack is fixedly arranged on the inner wall of the bottom of the detection box along the sliding direction of the slide plate, the first gear is fixedly sleeved on the bottom end of the support shaft, and the second gear is fixedly sleeved on the bottom end of the transmission shaft; the first gear is in meshing cooperation with the rack, and the second gear is in meshing cooperation with the first gear.

[0014] Preferably, the second transmission assembly includes a rotating disc, the rotating disc is fixedly sleeved on the top end of the transmission shaft, an eccentric shaft is fixedly arranged on the top of the rotating disc, the eccentric shaft is parallel to the transmission shaft and deviates from the transmission shaft, a horizontal groove is formed in the bottom of the placing plate, the eccentric shaft is inserted into the horizontal groove in the upward direction and is in driving cooperation with the placing plate, and a gap hole is formed in the slide plate and used for the eccentric shaft to move.

[0015] Preferably, two guide grooves are formed in the top of the slide plate, the two guide grooves are symmetrically arranged relative to the gap hole, the horizontal groove is located between the two guide grooves and is perpendicular to the two guide grooves, two sliding blocks are fixedly arranged on the bottom of the placing plate, the two sliding blocks correspond to the two guide grooves one by one, each sliding block is located in the corresponding guide groove and is in limiting sliding cooperation with the corresponding guide groove.

[0016] Preferably, a one-way bearing is arranged between the transmission shaft and the second gear; an inner ring of the one-way bearing is fixedly sleeved on the bottom end of the transmission shaft, and the second gear is fixedly sleeved on an outer ring of the one-way bearing.

[0017] Preferably, two guide rails are fixedly arranged on the inner wall of the detection box, and the sliding plate is transversely arranged between the two guide rails; the sliding plate is inserted into the tracks of the two guide rails and is in limited sliding cooperation with the two guide rails.

[0018] Preferably, a fixed shaft fixedly connected with the placement plate is vertically arranged on the placement plate; a plurality of placement grooves for placing test tubes are formed in the placement plate and are uniformly distributed along the circumference of the fixed shaft; and a plurality of limiting and clamping components for clamping the test tubes are fixedly arranged on the fixed shaft and correspond to the plurality of placement grooves in one-to-one correspondence.

[0019] Preferably, the limiting and clamping component comprises a horizontal U-shaped frame arranged along the radial direction of the fixed shaft and fixedly connected with the fixed shaft; two arc-shaped clamping plates for clamping the test tubes are mounted between the two distal ends of the U-shaped frame, the arc-shaped openings of the two arc-shaped clamping plates are opposite to each other and correspond to the two distal ends of the U-shaped frame in one-to-one correspondence; a support rod is fixedly arranged on the end portion of each arc-shaped clamping plate which is opposite to the other, and the support rod on each arc-shaped clamping plate penetrates through the distal end of the corresponding U-shaped frame and is in limited sliding cooperation with the distal end of the corresponding U-shaped frame; and a spring is fixedly arranged between each arc-shaped clamping plate and the distal end of the corresponding U-shaped frame.

[0020] The technical effects and advantages of the utility model are as follows: the reciprocating sliding of the placement plate and the sliding of the sliding plate are combined through the transmission mechanism, only a single electric push rod is needed to drive, the structure is simple, the control difficulty of the controller is reduced, the power consumption is reduced, and the detection cost is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is a three-dimensional structure schematic view of the utility model;

[0022] Figure 2 It is a detection box internal schematic view of the utility model;

[0023] Figure 3 It is a transmission mechanism schematic view of the utility model;

[0024] Figure 4 It is a limiting and clamping component schematic view of the utility model;

[0025] Figure 5 It is a guide rail schematic view of the utility model.

[0026] In the figure: 1, detection box; 2, mounting plate; 3, electric push rod; 4, sealing box door; 5, sliding plate; 6, rack; 7, first gear; 8, support shaft; 9, connecting rod; 10, transmission shaft; 11, one-way bearing; 12, second gear; 13, rotating disc; 14, eccentric shaft; 15, placing plate; 16, transverse groove; 17, sliding block; 18, guide groove; 19, fixed shaft; 20, U-shaped frame; 21, support rod; 22, spring; 23, arc-shaped clamping plate; 24, placing groove; 25, guide rail. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0028] The utility model provides a kind of device for integral fluorescent reaction as shown in Figures 1 to 5 The device includes a detection box 1. The detection box 1 has a device for fluorescence detection. The device is prior art and will not be described here.

[0029] Two horizontal guide rails 25 are fixedly arranged on the inner wall of the detection box 1, and a horizontal sliding plate 5 is transversely arranged between the two guide rails 25. The sliding plate 5 is inserted into the tracks of the two guide rails 25 and is limitedly slidably connected with the two guide rails 25.

[0030] A box opening is formed in the side of the detection box 1, and a sealing box door 4 is installed at the box opening. The sealing box door 4 is fixedly connected with the sliding plate 5. An installation plate 2 is fixedly arranged on the outer wall of the detection box 1, and an electric push rod 3 is fixedly installed on the installation plate 2. The electric push rod 3 is arranged along the sliding direction of the sliding plate 5, and the output shaft of the electric push rod 3 is fixedly connected with the sealing box door 4.

[0031] A controller is fixedly installed on the detection box 1, and the electric push rod 3 is electrically connected with the controller to control the start and stop of the electric push rod 3.

[0032] A placing plate 15 for placing test tubes is slidably installed on the top of the sliding plate 5. The test tubes are internally provided with samples and fluorescent dyes.

[0033] A transmission mechanism is installed between the bottom of the placing plate 15 and the detection box 1. The placing plate 15 is drivingly connected with the sliding plate 5 through the transmission mechanism, and the transmission mechanism is used to drive the placing plate 15 to reciprocally slide in the horizontal direction.

[0034] Specifically, the transmission mechanism comprises a vertical transmission shaft 10. The bottom of the sliding plate 5 is fixedly provided with a vertical support shaft 8, and a connecting rod 9 is fixedly arranged on the support shaft 8 in a radial direction. The transmission shaft 10 penetrates the end of the connecting rod 9 away from the support shaft 8 and is rotationally connected with the connecting rod 9.

[0035] The transmission shaft 10 is in transmission cooperation with the sliding plate 5 through a first transmission assembly, which is used to drive the transmission shaft 10 to rotate.

[0036] Specifically, the first transmission assembly comprises a first gear 7, a second gear 12 and a rack 6. The rack 6 is fixedly arranged on the inner wall of the bottom of the detection box 1 in the sliding direction of the sliding plate 5. The first gear 7 is fixedly sleeved on the bottom end of the support shaft 8, and the second gear 12 is fixedly sleeved on the bottom end of the transmission shaft 10. The first gear 7 is in meshing cooperation with the rack 6, and the second gear 12 is in meshing cooperation with the first gear 7.

[0037] The placement plate 15 is in transmission cooperation with the transmission shaft 10 through a second transmission assembly, which is used to drive the placement plate 15 to reciprocally slide in a horizontal direction.

[0038] Specifically, the second transmission assembly comprises a rotating disc 13, which is fixedly sleeved on the top end of the transmission shaft 10. The top of the rotating disc 13 is fixedly provided with an eccentric shaft 14, which is parallel to the transmission shaft 10 and deviates from the transmission shaft 10. The bottom of the placement plate 15 is provided with a horizontal groove 16, the eccentric shaft 14 is upwardly inserted into the horizontal groove 16 and is in transmission cooperation with the placement plate 15 in abutment. The sliding plate 5 is provided with a clearance hole for the eccentric shaft 14 to move, and the clearance hole is a circular hole and penetrates the sliding plate 5.

[0039] The top of the sliding plate 5 is provided with two guide grooves 18, which are symmetrically arranged relative to the clearance hole, and the horizontal groove 16 is located between the two guide grooves 18 and is perpendicular to the two guide grooves 18. The bottom of the placement plate 15 is fixedly provided with two sliding blocks 17, which correspond to the two guide grooves 18. Each sliding block 17 is located in the corresponding guide groove 18 and is in limiting sliding cooperation with the corresponding guide groove 18. In this embodiment, each sliding block 17 is a reverse T-shaped block, and each guide groove 18 matches the corresponding sliding block 17.

[0040] A one-way bearing 11 is arranged between the transmission shaft 10 and the second gear 12. The inner ring of the one-way bearing 11 is fixedly sleeved on the bottom end of the transmission shaft 10, and the second gear 12 is fixedly sleeved on the outer ring of the one-way bearing 11.

[0041] The top of the placement plate 15 is vertically provided with a fixed shaft 19 fixedly connected with the placement plate 15. The placement plate 15 is provided with a plurality of placement grooves 24 for placing test tubes, and the plurality of placement grooves 24 are uniformly distributed along the circumferential direction of the fixed shaft 19. A plurality of limiting and clamping assemblies for clamping test tubes are fixedly arranged on the fixed shaft 19, and the plurality of limiting and clamping assemblies correspond to the plurality of placement grooves 24.

[0042] Specifically, the limiting and clamping assembly comprises a horizontal U-shaped frame 20. The U-shaped frame 20 is arranged along the radial direction of the fixing shaft 19 and is fixedly connected with the fixing shaft 19. Two arc-shaped clamping plates 23 for clamping test tubes are arranged between the two distal ends of the U-shaped frame 20, and the arc openings of the two arc-shaped clamping plates 23 are opposite to each other and correspond to the two distal ends of the U-shaped frame 20 one by one.

[0043] The distal ends of the two arc-shaped clamping plates 23 are fixedly provided with a support rod 21, and the support rod 21 on each arc-shaped clamping plate 23 penetrates the distal end of the corresponding U-shaped frame 20 and is in limiting and sliding fit with the distal end of the corresponding U-shaped frame 20.

[0044] A spring 22 is fixedly arranged between each arc-shaped clamping plate 23 and the distal end of the corresponding U-shaped frame 20. A spring 22 is sleeved on each support rod 21.

[0045] Each test tube is clamped by two limiting and clamping assemblies arranged one above the other.

[0046] Working principle: when the integrated fluorescence reaction device is used for fluorescence detection, the controller controls the electric push rod 3 to start, the output shaft of the electric push rod 3 extends and drives the sealing box door 4 to move away from the detection box 1, and the sealing box door 4 gradually opens the box opening. At the same time, the sealing box door 4 drives the sliding plate 5 to slide out of the detection box 1, and the sliding plate 5 pushes the two sliding blocks 17 to move synchronously. The two sliding blocks 17 drive the placement plate 15 to move synchronously, and the placement plate 15 drives the fixing shaft 19 to move synchronously until all the placement grooves 24 on the placement plate 15 are moved out of the detection box 1. At this time, the controller controls the electric push rod 3 to stop. In this process, the sliding plate 5 drives the support shaft 8 to move synchronously, and the support shaft 8 drives the first gear 7 to move synchronously. The rack 6 drives the first gear 7 to rotate, the first gear 7 drives the second gear 12 to rotate, and the second gear 12 drives the outer ring of the one-way bearing 11 to rotate idly.

[0047] After that, the test tubes containing samples and fluorescent dyes are inserted into the corresponding placement grooves 24 through the two limiting and clamping assemblies. In this process, the operator can simultaneously push open the two arc-shaped clamping plates 23 of the two limiting and clamping assemblies. When the test tubes are inserted into the corresponding placement grooves 24, the arc-shaped clamping plates 23 of the two limiting and clamping assemblies are loosened. At this time, under the elastic force of the spring 22, the two arc-shaped clamping plates 23 of the two limiting and clamping assemblies clamp the test tubes.

[0048] Then, the controller controls the electric push rod 3 to start again, the output shaft of the electric push rod 3 retracts and drives the sealing box door 4 to move close to the detection box 1, and the sealing box door 4 gradually closes the box mouth. At the same time, the sealing box door 4 drives the sliding plate 5 to slide back to the detection box 1, and the sliding plate 5 drives the placing plate 15 to move back to the detection box 1 through the support shaft 8, the connecting rod 9, the transmission shaft 10, the rotating disc 13 and the eccentric shaft 14. The placing plate 15 drives the test tube to move into the detection box 1 through the placing groove 24 and the limiting clamping assembly on the fixed shaft 19.

[0049] In this process, the sliding plate 5 drives the support shaft 8 to move synchronously, and the support shaft 8 drives the first gear 7 to move synchronously. The rack 6 drives the first gear 7 to reverse, the first gear 7 drives the second gear 12 to reverse, and the second gear 12 drives the transmission shaft 10 to rotate synchronously through the one-way bearing 11. The transmission shaft 10 drives the rotating disc 13 to rotate synchronously, and the rotating disc 13 drives the eccentric shaft 14 to revolve around the transmission shaft 10. The eccentric shaft 14 drives the placing plate 15 to slide back and forth along the horizontal direction through the horizontal groove 16, and the placing plate 15 drives the fixed shaft 19 to move synchronously. The fixed shaft 19 drives the test tube to move back and forth through the limiting clamping assembly, and the sample and the fluorescent dye in the test tube are shaken, so that the sample and the fluorescent dye are rapidly mixed and reacted.

[0050] When the sealing box door 4 completely closes the box mouth, the controller controls the electric push rod 3 to stop. Then, the fluorescence detection can be performed by using the equipment for fluorescence detection in the detection box 1.

[0051] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, should be included in the protection scope of the present application.

Claims

1. A device for integrated fluorescent reaction, comprising a detection box (1) with equipment for fluorescent detection in the detection box (1), characterized in that: The detection box (1) is slidably installed with a horizontal sliding plate (5), and the top of the sliding plate (5) is slidably installed with a placing plate (15) for placing test tubes; A transmission mechanism is installed between the bottom of the placing plate (15) and the detection box (1), and the placing plate (15) is in transmission cooperation with the sliding plate (5) through the transmission mechanism, and the transmission mechanism is used to drive the placing plate (15) to reciprocally slide in the horizontal direction; A box opening is formed on the detection box (1) for the sliding plate (5) and the placing plate (15) to slide out, and a sealing box door (4) is installed at the box opening, and the sealing box door (4) is fixedly connected with the sliding plate (5); An electric push rod (3) is fixedly installed on the detection box (1), and the output shaft of the electric push rod (3) is fixedly connected with the sealing box door (4); A controller is fixedly installed on the detection box (1), and the electric push rod (3) is electrically connected with the controller.

2. The device of claim 1, wherein: The transmission mechanism comprises a vertical transmission shaft (10); the bottom of the sliding plate (5) is fixedly installed with a vertical support shaft (8), and a connecting rod (9) is fixedly arranged on the support shaft (8) in the radial direction; the transmission shaft (10) penetrates the end of the connecting rod (9) away from the support shaft (8) and is rotationally connected with the connecting rod (9); the transmission shaft (10) is in transmission cooperation with the sliding plate (5) through a first transmission assembly, and the first transmission assembly is used to drive the transmission shaft (10) to rotate; the placing plate (15) is in transmission cooperation with the transmission shaft (10) through a second transmission assembly, and the second transmission assembly is used to drive the placing plate (15) to reciprocally slide in the horizontal direction.

3. The device of claim 2, wherein: The first transmission assembly comprises a first gear (7), a second gear (12) and a rack (6); the rack (6) is fixedly arranged on the inner wall of the bottom of the detection box (1) in the sliding direction of the sliding plate (5); the first gear (7) is fixedly sleeved on the bottom end of the support shaft (8), and the second gear (12) is fixedly sleeved on the bottom end of the transmission shaft (10); the first gear (7) is in meshing cooperation with the rack (6), and the second gear (12) is in meshing cooperation with the first gear (7).

4. The device of claim 2, wherein: The second transmission assembly comprises a rotating disc (13), and the rotating disc (13) is fixedly sleeved on the top end of the transmission shaft (10); an eccentric shaft (14) is fixedly arranged on the top of the rotating disc (13), and the eccentric shaft (14) is parallel to and deviates from the transmission shaft (10); the bottom of the placing plate (15) is provided with a horizontal groove (16), the eccentric shaft (14) is upwardly inserted into the horizontal groove (16) and is in transmission cooperation with the placing plate (15); the sliding plate (5) is provided with a clearance hole for the eccentric shaft (14) to move.

5. The device of claim 4, wherein: The top of the sliding plate (5) is provided with two guide grooves (18), and the two guide grooves (18) are symmetrically arranged relative to the clearance hole; the horizontal groove (16) is located between the two guide grooves (18) and is perpendicular to the two guide grooves (18); the bottom of the placing plate (15) is fixedly provided with two sliding blocks (17), and the two sliding blocks (17) correspond to the two guide grooves (18) one by one; each sliding block (17) is located in the corresponding guide groove (18) and is in limiting sliding cooperation with the corresponding guide groove (18).

6. The device of claim 3, wherein: The one-way bearing (11) is arranged between the transmission shaft (10) and the second gear (12), the inner ring of the one-way bearing (11) is fixedly sleeved on the bottom end of the transmission shaft (10), and the second gear (12) is fixedly sleeved on the outer ring of the one-way bearing (11).

7. The device of claim 1, wherein: Two guide rails (25) are fixedly arranged on the inner wall of the detection box (1), and the sliding plate (5) is transversely arranged between the two guide rails (25); the sliding plate (5) is inserted into the tracks of the two guide rails (25) and is in limiting sliding cooperation with the two guide rails (25).

8. The device of claim 1, wherein: A fixing shaft (19) fixedly connected with the placing plate (15) is vertically arranged on the placing plate (15); a plurality of placing grooves (24) for placing test tubes are formed in the placing plate (15) and are uniformly distributed along the circumference of the fixing shaft (19); a plurality of limiting and clamping assemblies for clamping test tubes are fixedly arranged on the fixing shaft (19) and one-to-one correspond to the plurality of placing grooves (24).

9. The device of claim 8, wherein: The limiting and clamping assembly comprises a horizontal U-shaped frame (20) arranged along the radial direction of the fixing shaft (19) and fixedly connected with the fixing shaft (19); two arc-shaped clamping plates (23) for clamping test tubes are mounted between the two distal ends of the U-shaped frame (20), the arc-shaped openings of the two arc-shaped clamping plates (23) are opposite to each other and one-to-one correspond to the two distal ends of the U-shaped frame (20); a support rod (21) is fixedly arranged on the end portion of each arc-shaped clamping plate (23) which faces away from the other, the support rod (21) on each arc-shaped clamping plate (23) penetrates through the distal end of the corresponding U-shaped frame (20) and is in limiting sliding cooperation with the distal end of the corresponding U-shaped frame (20); a spring (22) is fixedly arranged between each arc-shaped clamping plate (23) and the distal end of the corresponding U-shaped frame (20).

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