Strain fermentation tank

Abstract

The utility model provides a strain fermentation tank, which relates to the technical field of strain processing devices, and comprises a tank body, the upper part and the bottom of the tank body are respectively communicated and fixedly provided with a feed pipe and a discharge pipe, and the bottom of the tank body is also communicated and fixedly provided with an air inlet pipe; the device further comprises a bacterium mixing mechanism, an air supplementing mechanism and a PH adjusting mechanism, the bacterium mixing mechanism comprises stirring blades, the stirring blades are transversely and rotatably arranged at the position, close to the bottom, of the tank body, and a driving source for driving the stirring blades to rotate is fixedly arranged outside the tank body; the air supply mechanism comprises an aeration ring, the aeration ring is fixedly arranged at the bottom in the tank body, a microporous aeration disc is arranged around the top of the aeration ring, an aeration ring pipe is communicated with the bottom of the aeration ring, the aeration ring pipe is communicated with the air inlet pipe, and the stirring blades are transversely arranged, so that the stirring blades generate a flow field guided by horizontal circulation, and the transverse shearing force and the shearing area are remarkably reduced; therefore, mechanical damage to hyphae caused by high-speed shearing is reduced, the hyphae can complete metabolic growth in a low-shearing environment, and the yield of a target product is increased.

Description

TECHNICAL FIELD

[0001] The utility model relates to a strain processing device technical field especially relates to a strain fermentation tank. BACKGROUND

[0002] Strain fermentation tank is the core equipment in the microbial fermentation process, mainly used for providing suitable growth environment (such as temperature, dissolved oxygen, pH value etc.) for strain, and promoting the uniform distribution of nutrient substances, strengthening the dissolved oxygen mass transfer efficiency through stirring, so as to improve the metabolic activity of bacteria and the product synthesis capacity.

[0003] Traditional fermentation tank usually adopts top drive type stirring mechanism, and the stirring shaft and paddle are rotated by the motor at the top end of the tank body, and the mechanical shear force is used to break the bubbles and mix the materials, to prevent the medium from settling and maintain the uniformity of the fluid in the tank, which plays an important role in the control of the density of the bacteria and the accumulation of the product, and is especially suitable for liquid fermentation system with high oxygen transfer efficiency.

[0004] However, the above-mentioned existing fermentation tank still has obvious defects in actual application: the stirring shaft driven at the top end produces transverse agitation due to the long cantilever, and the paddle forms a high-strength transverse shear field, which easily causes mechanical damage to fragile microorganisms such as mycelium, resulting in mycelium breakage and intracellular substance leakage, which not only reduces the activity of the bacteria, but also may change the metabolic flow direction and affect the yield of the target product. UTILITY MODEL CONTENTS

[0005] In view of the deficiencies in the prior art, the utility model provides a strain fermentation tank, which solves the problem of high-strength transverse shear field formed by the paddle, stirring shear damage to mycelium and influence on the yield of target product in the prior art.

[0006] According to the embodiment of the utility model, a strain fermentation tank comprises:

[0007] A tank body is fixedly arranged, and an inlet pipe and an outlet pipe are respectively communicated with and fixedly arranged at the upper part and the bottom part of the tank body, and an air inlet pipe is further communicated with and fixedly arranged at the bottom part of the tank body;

[0008] A mixed bacteria mechanism comprises a stirring blade, which is arranged to rotate transversely at the bottom part of the tank body, and a driving source is fixedly arranged outside the tank body to drive the stirring blade to rotate;

[0009] A gas supplementing mechanism comprises an aeration ring, which is fixedly arranged at the inner bottom part of the tank body, and a plurality of microporous aeration discs are fixedly arranged around the aeration ring, and an aeration ring pipe is communicated with the bottom part of the microporous aeration disc and the air inlet pipe;

[0010] A PH adjusting mechanism is in communication with and fixedly arranged on the side wall of the tank body for adjusting the PH in the tank body.

[0011] The technical principle of the utility model is as follows: the bacteria liquid is input into the tank body through the feeding pipe, the driving source is started to drive the stirring blade to rotate, the stirring blade rotation generates a horizontal circulation dominant flow field, the bacteria liquid is uniformly mixed, meanwhile, the aeration ring is started, a large amount of micro-bubbles are generated through the micro-porous aeration disc, the micro-bubbles are affected by the flow field generated by the stirring blade when rising and moving with the bacteria liquid, the residence time is increased, and the oxygen dissolution efficiency is improved.

[0012] Further, the stirring blade structure comprises a backward-swept three-blade curved paddle, and the edge of the stirring blade is subjected to streamline blunting treatment.

[0013] Further, the driving source comprises a motor, and the output shaft of the motor is connected with the stirring blade.

[0014] Further, the tank body is further fixedly connected with a speed reducer, one side of the stirring blade is fixedly connected with a rotating shaft, the rotating shaft penetrates through the tank body and is fixedly connected with the driven end of the speed reducer, and the output shaft of the motor is fixedly connected with the driving end of the speed reducer.

[0015] Further, the PH adjusting mechanism comprises an acid inlet pipe and an alkali inlet pipe, and the acid inlet pipe and the alkali inlet pipe are in communication with the tank body respectively.

[0016] Still further, the PH adjusting mechanism further comprises a pH meter, and the pH meter is in communication with and fixedly arranged on the side wall of the tank body.

[0017] Still further, a plurality of micro-holes are uniformly arranged on the micro-porous aeration disc.

[0018] Still further, a top cover is detachably arranged on the top of the tank body, a pressure regulating valve is fixedly arranged at the top end of the top cover, and a pressure gauge is in communication with and fixedly arranged on one side of the pressure regulating valve.

[0019] Still further, an observation port is further in communication with and fixedly arranged on one side of the top of the top cover.

[0020] Still further, a liquid level meter and a temperature gauge are in communication with and arranged on the side wall of the tank body.

[0021] Compared with the prior art, the utility model has the following beneficial effects:

[0022] 1. The transversely arranged side-in type stirring blade generates a horizontal circulation dominant flow field, the transverse shear force and shear area are significantly reduced, the mechanical damage of mycelium caused by high-speed shearing is reduced, the mycelium is mainly subjected to mild radial impact instead of high shear impact in the bacteria liquid, the mycelium completes metabolic growth in a low shear environment, and the target product yield is improved.

[0023] 2. By using a microporous aeration disc arranged around the aeration ring, uniform and dense microbubbles can be generated at the bottom of the tank. As the bubbles rise, they come into contact with the horizontal circulation, which lengthens the bubble trajectory, increases the average residence time, improves the gas-liquid mass transfer interface renewal frequency, enhances dissolved oxygen efficiency, and increases the yield of the target product. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model.

[0025] Figure 2 This is a cross-sectional structural diagram of an embodiment of the present utility model.

[0026] Figure 3 This is a schematic diagram of the mixed bacteria mechanism according to an embodiment of the present invention.

[0027] Figure 4 This is a schematic diagram of the aeration disc structure according to an embodiment of the present invention.

[0028] Figure 5 This is a schematic diagram of the control principle of the pH adjustment mechanism in an embodiment of this utility model.

[0029] In the above attached diagrams: 1. Tank body; 11. Top cover; 111. Pressure regulating valve; 112. Pressure gauge; 113. Observation port; 12. Feed pipe; 13. Level gauge; 14. Sight glass; 15. Thermometer; 16. pH meter; 17. pH adjustment mechanism; 171. Acid inlet pipe; 172. Alkali inlet pipe; 18. Discharge pipe; 181. Pneumatic valve; 19. Support leg; 2. Mixing mechanism; 21. Stirring blade; 22. Rotating shaft; 23. Protective cover; 24. Reducer; 25. Motor; 3. Air supply mechanism; 31. Air inlet pipe; 32. Microporous aeration disc; 33. Aeration ring pipe; 34. Aeration ring. Detailed Implementation

[0030] The technical solution of this utility model will be further described below with reference to the accompanying drawings and embodiments.

[0031] like Figure 1 and Figure 2 As shown in the figure, this utility model embodiment proposes a microbial fermentation tank, including a tank body 1. Two pairs of support legs 19 are symmetrically fixed at the bottom of the tank body 1, so that the tank body 1 is horizontally fixed. The upper side wall and the bottom of the tank body 1 are respectively fixedly provided with an inlet pipe 12 and an outlet pipe 18 communicating with the inside and outside. The inlet pipe 12 and the outlet pipe 18 are respectively connected to the microbial liquid conveying pipes from the upper and lower processes. A pneumatic valve 181 for controlling the opening and closing of the pipe is fixedly provided on the outlet pipe 18. An air inlet pipe 31 is also fixedly provided at the bottom of the tank body 1 for replenishing air.

[0032] likeFigure 1 and Figure 2 As shown in the figure, this utility model embodiment proposes a microbial fermentation tank, which also includes a mixing mechanism 2. The mixing mechanism 2 includes a stirring blade 21, which is laterally rotatably disposed near the bottom of the tank body 1. The stirring blade 21 is preferably made of titanium alloy. The structure of the stirring blade 21 is a swept-back three-bladed curved blade. The edges of the stirring blade 21 are streamlined and passivated so that when the stirring blade 21 rotates, the radially moving bacterial liquid can form a mild flow field dominated by horizontal circulation after touching the inner wall of the curved surface of the tank body 1, which significantly reduces the lateral shear force and shear area, thereby reducing the mechanical damage of high-speed shear to the hyphae. At the same time, a drive source for driving the stirring blade 21 to rotate is fixedly disposed outside the tank body 1. In this embodiment, the drive source is a motor 25, and the output shaft of the motor 25 is connected to the stirring blade 21.

[0033] like Figures 1-3 As shown in this embodiment, to further convert the high speed of the motor 25 to a low speed suitable for mycelial growth while increasing the output torque, a reducer 24 is fixedly connected to the outside of the tank body 1. A rotating shaft 22 is fixedly connected to one side of the stirring blade 21. The rotating shaft 22 passes through the tank body 1 and is fixedly connected to the driven end of the reducer 24. A protective cover 23 for protecting the rotating shaft 22 is fixedly installed on the reducer 24. The output shaft of the motor 25 is fixedly connected to the drive end of the reducer 24, so that when the rotating shaft 22 of the motor 25 rotates, the high-speed low-torque power output can be converted into low-speed high-torque power through the reducer 24 and transmitted to the rotating shaft 22, thereby driving the stirring blade 21 to rotate. This achieves the purpose of preventing culture medium sedimentation, mixing the mycelium, and maintaining the uniformity of the fluid in the tank. Furthermore, multiple mixing mechanisms 2 can be arranged around the outer wall of the tank body 1 near the bottom. The stirring blades 21 of multiple mixing mechanisms 2 can be driven simultaneously to form a composite flow field, thereby further improving the mixing effect.

[0034] like Figures 1-2 and Figure 4As shown, the utility model embodiment proposes a kind of strain fermentation tank, still including air supplement mechanism 3, the air supplement mechanism 3 includes aeration ring 34, the aeration ring 34 is fixedly arranged in the tank body 1 inner bottom around the discharge pipe 18, the aeration ring 34 bottom is connectedly provided with the aeration ring pipe 33 of circular ring, the aeration ring pipe 33 is coaxially fixedly arranged in the tank body 1 bottom, and with the air inlet pipe 31 communication, the aeration ring 34 top is fixedly provided with several microporous aeration disc 32 in circumferential array, the microporous aeration disc 32 is detachably fixed with the aeration ring 34 by bolt, to facilitate the microporous aeration disc 32 replacement, the microporous aeration disc 32 is evenly provided with several micropores, can be selected according to actual use demand to micropore aperture, the micropore aperture is preferably set as 50 μm or so, to produce uniform and dense microbubble for bacteria liquid oxygen supply, the microporous aeration disc 32 bottom is connected with the aeration ring pipe 33, improves the efficiency and effect of dissolved oxygen.

[0035] As Figures 1-2 As shown, in the embodiment, further, the tank body 1 one side is provided with transparent sight glass 14, can observe tank body 1 internal condition through the sight glass 14 in production process, the tank body 1 top is detachably provided with top cover 11, the top cover 11 can be sealed and fixed on the tank body 1 by bolt, to facilitate the tank body 1 to be removed when being overhauled or maintained, the top cover 11 top one side is connected and fixedly provided with observation port 113, the observation port 113 is hinged with sealing cover, the sealing cover one side is fixedly provided with valve for controlling sealing cover opening and closing, the valve is preferably set as normally closed, can first judge tank body 1 internal condition through the observation port 113 when being overhauled or maintained, improve the efficiency of overhaul or maintenance, further, the top cover 11 top end is fixedly provided with pressure regulating valve 111, the pressure regulating valve 111 can balance the air pressure in the tank body 1, prevent pressure being too high, the pressure regulating valve 111 one side is connected and fixedly provided with pressure gauge 112, for monitoring the pressure in the tank body 1, the tank body 1 side wall is also connected and provided with liquid level gauge 13 and temperature gauge 15, for monitoring the liquid level and temperature in the tank body 1, provide suitable environment for the strain propagation.

[0036] As Figures 1-2As shown, in the embodiment, further, the tank body 1 side wall is further provided with a PH adjusting mechanism 17, the PH adjusting mechanism 17 comprises an acid inlet pipe 171 and an alkali inlet pipe 172, the acid inlet pipe 171 and the alkali inlet pipe 172 are communicated with the tank body 1 respectively, the acid inlet pipe 171 and the alkali inlet pipe 172 are respectively provided with an acid liquid pipeline and an alkali liquid pipeline for conveying acid liquid and alkali liquid, the acid liquid pipeline and the alkali liquid pipeline are respectively provided with an acid liquid conveying pump and an alkali liquid conveying pump, the acid liquid conveying pump and the alkali liquid conveying pump are preferably pneumatic pumps, the tank body 1 side wall is further communicated and fixedly provided with a PH meter 16, for monitoring the PH in the tank body 1, for further realizing intelligent control, such as Figure 5 As shown, the PH meter 16 is further electrically connected with a data processing element, the data processing element is electrically connected with the acid liquid conveying pump and the alkali liquid conveying pump, the data processing element can receive an electric signal from the PH meter 16 and output the electric signal to the acid liquid conveying pump or the alkali liquid conveying pump after processing, for controlling the input of acid liquid or alkali liquid, and further adjusting the PH of the bacteria liquid in the tank body 1, specifically, first, the PH range or threshold value is preset through the data processing element, when the data processing unit receives the electric signal from the PH meter 16 and detects that the PH in the tank body exceeds the range or reaches the corresponding threshold value, the data processing element will output the electric signal to the acid liquid conveying pump or the alkali liquid conveying pump after analysis and processing, the acid liquid conveying pump or the alkali liquid conveying pump starts to supplement acid liquid or alkali liquid into the bacteria liquid in the tank body 1, so that the PH in the tank body is always maintained in the range optimal for the growth of bacteria, and a suitable environment is provided for the propagation of bacteria.

[0037] The technical principle of the utility model is: through the feed pipe 12, the bacteria liquid is input into the tank body 1, the motor 25 is started to drive the stirring blade 21 to rotate, the stirring blade 21 rotates to generate a horizontal circulation dominant mild flow field, promotes the uniform mixing of bacteria liquid, at the same time, the aeration ring 34 is started, a large number of micro-bubbles are generated in the microporous aeration disc 32, the micro-bubbles are affected by the flow field generated by the stirring blade 21 when rising, and are mixed with the bacteria liquid, the residence time is increased, and the oxygen dissolution efficiency and effect are improved.

[0038] The utility model discloses a horizontal side -entry type stirring blade 21 makes, and stirring blade 21 produces horizontal ring flow dominant flow field, significantly reduces lateral shear force and shearing area to reduce the mechanical damage of high -speed shearing to mycelium, and mycelium is mainly subjected to mild radial impact in broth instead of high shear impact, and mycelium completes metabolic growth in low shear environment, through the micro -bubble aerator 32 of surrounding setting on the aeration ring 34, can produce uniform and dense micro -bubble at the bottom of jar body 1, and the bubble is contacted with horizontal ring flow in the rising process, makes the bubble motion trajectory lengthen, and average residence time increases, and the renewal frequency of gas -liquid mass transfer interface promotes, and the efficiency of dissolved oxygen improves, and the target product yield is improved, and the volume that stirring blade 21 occupies is smaller and simpler compared to traditional stirring mechanism, and it is more favorable to the cleaning and later maintenance of jar body 1, and further reduce production cost.

[0039] Finally, it is explained that the above examples are only used to illustrate the technical solutions of the utility model and are not limited. Although the utility model has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the utility model can be modified or replaced equivalently without departing from the purpose and scope of the technical solutions of the utility model, and they should be covered in the scope of the claims of the utility model.

Claims

1. A strain fermentation tank characterized by comprising: The utility model relates to a kind of multi-strain fermentation tank, including: Tank body (1), the upper portion and bottom of the tank body (1) are respectively communicated and fixedly provided with feed pipe (12) and discharge pipe (18), the bottom of the tank body (1) is also communicated and fixedly provided with air inlet pipe (31); Multi-strain mechanism (2), the stirring blade (21) is transversely rotatably arranged at the bottom of the tank body (1), and the driving source for driving the stirring blade (21) is fixedly arranged outside the tank body (1); Air supplement mechanism (3), the aeration ring (34) is fixedly arranged in the bottom of the tank body (1), and a plurality of microporous aeration discs (32) are fixedly arranged around the aeration ring (34), the bottom of the microporous aeration disc (32) is communicated with aeration ring pipe (33), and the aeration ring pipe (33) is communicated with the air inlet pipe (31); PH adjusting mechanism (17), the PH adjusting mechanism (17) is communicated and fixedly arranged on the side wall of the tank body (1), for adjusting the PH in the tank body.

2. The strain fermentation tank according to claim 1, characterized by: The stirring blade (21) structure includes a backward-swept three-blade curved surface paddle, and the edge of the stirring blade (21) is subjected to streamline blunting treatment.

3. The strain fermenter according to claim 1, wherein: The driving source includes motor (25), and the output shaft of the motor (25) is connected with the stirring blade (21).

4. The strain fermenter according to claim 3, wherein: The tank body (1) is also fixedly connected with a speed reducer (24) outside, one side of the stirring blade (21) is fixedly connected with a rotating shaft (22), the rotating shaft (22) penetrates the tank body (1) and is fixedly connected with the driven end of the speed reducer (24), and the output shaft of the motor (25) is fixedly connected with the driving end of the speed reducer (24).

5. The strain fermenter according to claim 1, wherein: The PH adjusting mechanism (17) includes acid inlet pipe (171) and alkali inlet pipe (172), and the acid inlet pipe (171) and the alkali inlet pipe (172) are communicated with the tank body (1) respectively.

6. A seed fermenter as claimed in claim 5, characterised in that: The PH adjusting mechanism (17) further includes a pH meter (16), and the pH meter (16) is communicated and fixedly arranged on the side wall of the tank body (1).

7. The strain fermenter according to claim 1, wherein: A plurality of micropores are uniformly arranged on the microporous aeration disc (32).

8. The strain fermenter according to claim 1, wherein: The top of the tank body (1) is detachably provided with a top cover (11), the top end of the top cover (11) is fixedly provided with a pressure regulating valve (111), one side of the pressure regulating valve (111) is communicated and fixedly provided with a pressure gauge (112).

9. A seed fermenter as claimed in claim 8, characterised in that: The top of the top cover (11) is also communicated and fixedly provided with a viewing port (113) on one side.

10. The strain fermenter according to claim 1, wherein: The liquid level meter (13) and the temperature gauge (15) are communicated and arranged on the side wall of the tank body (1).

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