Plant elisa detection heating box

Abstract

The utility model discloses a plant elisa detection heating box which comprises a base, a cover and a heating mechanism, the cover is hinged to the base, a heating plane is arranged in the base and made of heat conduction materials, the heating mechanism is arranged in the base and makes contact with the heating plane so that heat can be transmitted to the heating plane, the cover is light-proof, and the heating mechanism is arranged in the base and makes contact with the heating plane. And the heating plane is used for placing the microwell plate so as to heat the microwell plate. Compared with the prior art, by arranging the heating plane and the heating mechanism, the micro-pore plate or the kit on the heating plane is heated, so that the micro-pore plate is always kept in a constant-temperature condition in the developing process, the detection accuracy is ensured, the waiting time can be shortened by heating the kit, and the detection efficiency is improved. Therefore, detection requirements can be extracted as soon as possible, and detection accuracy is guaranteed.

Description

Technical Field

[0001] This utility model relates to a detection device, and more particularly to a heating box for plant ELISA detection used in substrate colorimetric reactions. Background Technology

[0002] Currently, the detection process of plant ELISA kits is mainly carried out in microplates. After the sample and reagents react, a colored substance is produced. The absorbance value at a specific wavelength is measured, and the content of the substance in the sample is calculated according to a standard curve. The detection process requires the addition of an enzyme substrate. The substrate is catalyzed by the enzyme to become a colored product. This needs to be carried out at room temperature or 37°C. After the substrate color reaction takes 30 minutes, a stop solution is added to turn the blue color to a dark yellow. The mixture is then thoroughly mixed by shaking, and finally, the result is measured on a microplate reader. Since this test may be performed outside the laboratory, the room temperature cannot be guaranteed to be 20-25°C or 37°C, which can lead to inaccurate results and fail to meet detection requirements. Furthermore, because the kit is refrigerated, it needs to be left at room temperature for 15-30 minutes after removal before use, which also prolongs the operation time. Utility Model Content

[0003] The purpose of this invention is to provide a heating box for plant ELISA detection, and the technical problem to be solved is to achieve heating during the colorimetric reaction of the reagent kit or substrate.

[0004] To solve the above problems, the present invention adopts the following technical solution: a heating box for plant ELISA detection, including a base, a cover, and a heating mechanism. The cover is hinged to the base, and a heating plane is provided in the base. The heating plane is made of a thermally conductive material. The heating mechanism is located in the base and contacts the heating plane to transfer heat to the heating plane. The cover is opaque. A microplate is placed on the heating plane to heat the microplate. The heating plane is a harmonica tube made of aluminum alloy. The inner cavity of the heating plane is filled with a refrigerant. Both ends of the heating plane are provided with sealing surfaces to seal the inner cavity.

[0005] Furthermore, the heating mechanism includes a PTC heating component, a temperature sensor, and a control board. The temperature sensor is disposed on the heating plane, the PTC heating component includes at least one heating surface that is in contact with the heating plane, and the control board is connected to the PTC heating component and the temperature sensor respectively.

[0006] Furthermore, the heating mechanism also includes a power supply, which is connected to the PTC heating component, the temperature sensor, and the control board.

[0007] Furthermore, the heating source of the heating mechanism is located at the center of the heating plane.

[0008] Furthermore, the PTC heating assembly includes multiple PTC heating elements, an electrode plate sandwiching the PTC heating elements in the middle, insulating paper wrapped around the electrode plate, and a heat-conducting aluminum tube.

[0009] Compared with the prior art, this invention heats the microplate or reagent kit placed on the heating plane by setting a heating plane and a heating mechanism, so that the microplate is kept at a constant temperature during the color development process, ensuring the accuracy of the detection. Heating the reagent kit reduces the waiting time, thereby meeting the requirement of early extraction and detection and ensuring the accuracy of the detection. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the external structure of this utility model.

[0011] Figure 2 This is a schematic diagram of the internal structure of this utility model.

[0012] Figure 3 This is a schematic diagram of the structure of the PTC heating assembly of this utility model. Detailed Implementation

[0013] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0014] like Figure 1 and Figure 2 As shown, this utility model discloses a heating box for plant ELISA detection, including a base 1, a cover 2, and a heating mechanism 3. The cover 2 is hinged to one end of the base 1. The base 1 has a cavity and an opening. A heating plane 4 is provided in the base 1, close to the opening of the base 1. The heating plane 4 is made of a thermally conductive material. The heating mechanism 3 is located in the base 1 and contacts the heating plane 4 to transfer heat to the heating plane 4, thereby satisfying the requirement of heating the entire microplate. The cover 2 is opaque, specifically made of opaque plastic, to meet the requirement of partial opacity. In this utility model, the heating temperature of the heating mechanism 3 is based on the detection requirements, generally between 20-37℃. The heating plane 4 can also heat the reagent kit taken from the refrigerated environment to room temperature, which is generally between 20-25℃.

[0015] like Figure 2As shown, the heating mechanism 3 includes a PTC heating element 5, a temperature sensor 6, and a control board 7. The temperature sensor 6 is located on the heating plane 4 to detect the temperature of the heating plane 4, ensuring that the temperature of the heating plane 4 is maintained at the set temperature. The control board 7 has buttons and a display screen connected to it. The buttons are located on the base 1 to realize temperature adjustment and on / off functions. The PTC heating element 5 includes at least one heating surface that is in contact with the heating plane 4. The control board 7 is connected to the PTC heating element 5 and the temperature sensor 6 respectively. Specifically, the heating mechanism 3 also includes a power supply 12, which is connected to the PTC heating element 5, the temperature sensor 6, and the control board 7 respectively. The power supply 12 can be a rechargeable battery. The housing 1 has a charging interface for charging the power supply 12 to meet wireless use in different scenarios.

[0016] In this invention, the heating plane 4 covers the opening of the entire box 1, and the heating plate 4 can be made of aluminum alloy.

[0017] In this invention, the heating plane 4 is a harmonica tube made of aluminum alloy. The inner cavity of the heating plane 4 is filled with refrigerant. Both ends of the heating plane 4 are provided with sealing surfaces to seal the inner cavity. The use of a harmonica tube and the filling with refrigerant can quickly achieve heat conduction to the entire heating plane.

[0018] In this invention, the PTC heating component 5 is located in the middle of the heating plane 4 to enable rapid heat conduction to both sides.

[0019] like Figure 3 As shown, the PTC heating assembly 5 includes multiple PTC heating elements 8, an electrode plate 9 sandwiching the PTC heating elements 8 in the middle, insulating paper 10 wrapped around the electrode plate 9, and a heat-conducting aluminum tube 11. Specifically, the PTC heating elements 8 are arranged in a row, and the conductive coating on the PTC heating elements 8 is connected to the electrode plate 9. The electrode plate 9 is provided with two pieces, namely a positive electrode and a negative electrode. The insulating paper 10 is wrapped around the electrode plate 9. Finally, the heat-conducting aluminum tube 11 is sleeved on the insulating paper 10 and pressed tightly. The length direction of the PTC heating assembly 5 is perpendicular to the length direction of the inner cavity of the heating plane 4, so that heat can be conducted to both sides of the length direction of the inner cavity.

[0020] In use, the PTC heating element 5 is first turned on to maintain its operating temperature at room temperature (20-25 degrees Celsius). The reagent kit taken from the refrigerator is placed on the heating surface 4 to prepare the plant specimen. During the substrate colorimetric reaction, the microplate is placed on the heating surface 4, the lid is sealed, and after 30 minutes, the stop solution is added. The microplate is then removed from the heating surface 4 and measured using a microplate reader. Of course, light protection is required during the colorimetric reaction, and since the lid is made of an opaque material, this requirement is met.

[0021] This invention enables the reagent kit to be used in a short time, addressing the experimental requirement of extraction as soon as possible after sample collection. Heating the substrate during the colorimetric reaction ensures the accuracy of color development and detection.

Claims

1. A plant elisa detection heating cartridge, characterized by: The application relates to a heating device for a microplate, which comprises a base (1), a cover (2), a heating mechanism (3), the cover (2) is hinged to the base (1), a heating plane (4) is arranged in the base (1), the heating plane (4) is made of a heat-conducting material, the heating mechanism (3) is arranged in the base (1) and is in contact with the heating plane (4) to realize heat transfer to the heating plane (4), the cover (2) is light-proof, and the heating plane (4) is used for placing a microplate to heat the microplate; the heating plane (4) is a harmonica tube made of an aluminum alloy material, refrigerant is filled in an inner cavity of the heating plane (4), and both ends of the heating plane (4) are provided with sealing surfaces to seal the inner cavity.

2. The plant ELISA test heating cartridge of claim 1, wherein: The heating mechanism (3) comprises a PTC heating assembly (5), a temperature sensor (6) and a control board (7), the temperature sensor (6) is arranged on the heating plane (4), the PTC heating assembly (5) comprises at least one heating surface, the heating surface is attached to the heating plane (4), and the control board (7) is connected with the PTC heating assembly (5) and the temperature sensor (6) respectively.

3. The plant ELISA test heating cartridge of claim 1, wherein: The heating mechanism (3) further comprises a power supply (12), the power supply (12) is connected with the PTC heating assembly (5), the temperature sensor (6) and the control board (7) respectively.

4. The plant ELISA test heating cartridge of claim 1, wherein: The heating source of the heating mechanism (3) is arranged at a middle position of the heating plane (4).

5. The plant ELISA test heating cartridge of claim 2, wherein: The PTC heating assembly (5) comprises a plurality of PTC heating elements (8), an electrode plate (9) clamping the PTC heating elements (8), insulating paper (10) wrapped outside the electrode plate (9) and a heat-conducting aluminum pipe (11).

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