Low-temperature uniform mixing device
By using the cooling plate and extrusion head assembly of the low-temperature mixing device, the problem of low-temperature mixing in the prior art has been solved, achieving uniform cell distribution and high-precision filling, thereby improving cell viability and filling accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-23
- Publication Date
- 2026-04-03
AI Technical Summary
Existing mixing devices cannot operate at low temperatures, resulting in reduced cell viability at room or high temperatures. Furthermore, existing mixing methods suffer from high shear forces, mixing dead zones, and air bubbles, which affect cell quality and filling accuracy.
A low-temperature mixing device was designed, including a cooling plate and a reciprocating extrusion device. The cooling plate maintains a low-temperature environment, and the extrusion head and roller assembly are used to squeeze and alternately squeeze the liquid storage bag to ensure uniform cell distribution and filling accuracy.
Uniform cell distribution was achieved under low-temperature conditions, reducing shear force damage to cells, improving filling accuracy and cell viability, preventing air bubbles from entering, and ensuring cell quality.
Smart Images
Figure CN224071853U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a mixing device, and more particularly to a cell low-temperature mixing device. Background Technology
[0002] In the field of cell therapy, formulation filling is the last important step in the preparation process. The uniformity and quality of cell formulations are important indicators of concern to customers. In the process of cell fluid filling, the cell fluid is first put into the storage bag, and then the tubing connected to the storage bag is installed on the peristaltic pump, and the filling needle is installed on the follow-up mechanism of the filling equipment. In order to achieve cell uniformity, the storage bag containing cell fluid needs to be mixed manually or placed on a swing mixing device. Mixing and filling are carried out simultaneously. However, the existing mixing devices have the following problems: (1) They work at room temperature and cannot meet the requirements of cell fluid that needs to be stored at low temperature, because it takes a certain amount of time from the start of filling to completion. During this process, if the cells are in a room temperature or high temperature environment for a long time, the cell viability will decrease, which will seriously affect the cell quality; (2) Existing mixing methods, such as stirring, require a stirring paddle in the storage bag, and the mixing process will have large shear forces and mixing dead corners, resulting in problems such as uneven cell density and low cell quality. In addition, in the later stage of filling, due to the reduction of liquid in the storage bag and the effect of cell gravity, it is difficult to achieve uniform mixing of cell preparations; (3) Existing methods for achieving uniform mixing, such as rocker shaking and oscillation method, and rotary reciprocating circular shaking method, are used. The storage bag is placed flat. During the process of mixing and filling, air bubbles are easily introduced into the pipeline, resulting in the actual filling volume being lower than the set volume, i.e., the filling volume deviation is large. Summary of the Invention
[0003] To address the aforementioned problems, this utility model provides a low-temperature mixing device, comprising a cooling plate, an outer frame, and a reciprocating extrusion device. The cooling plate is located within the outer frame, and the reciprocating extrusion device is used to press the liquid storage bag against the cooling plate and to extrude the liquid storage bag.
[0004] Furthermore, the low-temperature mixing device also includes an extrusion head assembly, which is disposed at the lower part of the cooling plate. The extrusion head assembly includes multiple extrusion heads that can extend and retract relative to the outer surface of the cooling plate.
[0005] Furthermore, the reciprocating extrusion device includes: a roller assembly and a movable frame. The movable frame includes a first support plate and a second support plate arranged opposite to each other. The roller assembly includes rollers and bearings. The bearings pass through the rollers. One end of the bearings is pivotally connected to the first support plate, and the other end of the bearings is locked to the second support plate.
[0006] Furthermore, the movable frame can move up and down and back and forth relative to the cooling plate.
[0007] Furthermore, the other end of the bearing is magnetically locked to the second support plate.
[0008] Furthermore, the end of the second support plate is provided with a slot, and the other end of the bearing has a first locking hole. At least one side of the slot is provided with a second locking hole corresponding to the first locking hole. When the other end of the bearing is inserted into the slot, a pin is used to pass through the first locking hole and the second locking hole to lock the other end of the bearing to the second support plate.
[0009] Furthermore, the reciprocating extrusion device includes: a roller assembly and a rocking arm. The rocking arm includes a first rocking plate and a second rocking plate arranged opposite each other. The roller assembly includes three rollers arranged at the top, middle, and bottom, and three bearings passing through the three rollers respectively. Gaskets are provided on both sides of the three rollers. The bearings of the upper and lower rollers are fixedly connected to the gaskets at both ends. The bearings of the middle roller pass through the gaskets on both sides and are fixedly connected to the front ends of the first rocking plate and the second rocking plate respectively. The rear ends of the first rocking plate and the second rocking plate are connected to a drive mechanism on the back of the outer frame. The drive mechanism can drive the first rocking plate and the second rocking plate to reciprocate around the axis.
[0010] Furthermore, the reciprocating extrusion device includes a bag pressing mechanism, which includes left and right swing arms and a pressure plate. The two ends of the pressure plate are connected to the lower parts of the left and right swing arms, and the upper parts of the swing arms are hinged to the upper left and right sides of the outer frame. A torsion spring is sleeved on the hinge shaft so that the pressure plate always presses against the cooling plate.
[0011] Furthermore, the reciprocating extrusion device also includes: a rack, a gear, a connecting rod, and a support plate. The rack is set on the back of the outer frame and extends vertically. The gear is arranged on the support plate and meshes with the rack. One end of the connecting rod is hinged to the lower part of the rack, and the other end is hinged to the support plate. Driven by the repeated forward and reverse rotation of the gear, the cooling plate moves periodically in an approximately S-shaped trajectory relative to the support plate through the movement of the rack and the connecting rod.
[0012] Furthermore, the reciprocating extrusion device includes: a support frame, a rocking frame, and a bag pressing mechanism. The rocking frame is fixed to the back of the outer frame, and a bushing is provided on the rocking frame. The support frame is provided with a corresponding shaft hole, and the shaft passes through the shaft hole and is inserted into the bushing, so that the rocking frame can pivot relative to the support frame.
[0013] The top of the outer frame is provided with a first arc-shaped groove and a second arc-shaped groove, which are located on both sides of the shaft. A first pin is disposed in the first arc-shaped groove, and a second pin is disposed in the second arc-shaped groove. The bag-pressing mechanism includes a first connecting rod and a second connecting rod. The two ends of the first connecting rod are hinged to the shaft and the first pin, respectively, and the two ends of the second connecting rod are hinged to the shaft and the second pin, respectively. The first pin and the second pin can slide within the first arc-shaped groove and the second arc-shaped groove, respectively.
[0014] The bag pressing mechanism also includes a third link and a fourth link. One end of the third link is hinged to the first pin and the other end is hinged to the upper end of the first roller. One end of the fourth link is hinged to the second pin and the other end is hinged to the upper end of the second roller. The first roller and the second roller extend vertically along the outer frame. The liquid storage bag is sandwiched between the first roller, the second roller and the cooling plate.
[0015] Furthermore, at the bottom of the outer frame, a third arc-shaped groove corresponding to the first arc-shaped groove and a fourth arc-shaped groove corresponding to the second arc-shaped groove are symmetrically provided. The bag pressing mechanism is also symmetrically provided at the bottom of the outer frame with a fifth link, a sixth link, a seventh link, and an eighth link respectively corresponding to the first link, the second link, the third link, and the fourth link.
[0016] The fifth connecting rod is hinged at one end to the shaft and at the other end to the third pin, which is located within the third arc-shaped groove. The seventh connecting rod is hinged at one end to the third pin and at the other end to the lower end of the first roller. The sixth connecting rod is hinged at one end to the shaft and at the other end to the fourth pin, which is located within the fourth arc-shaped groove. The eighth connecting rod is hinged at one end to the fourth pin and at the other end to the lower end of the second roller.
[0017] This invention addresses the need for reagents requiring low-temperature filling by incorporating a cooling plate. A reciprocating extrusion device presses the storage bag against the cooling plate while simultaneously squeezing the cell-filled bag, ensuring uniform cell distribution and overcoming the problem of high shear force and cell damage caused by mixing methods like stirring in existing technologies. Furthermore, the addition of alternating reciprocating extrusion heads in the later stages of filling facilitates mixing of the cell preparation, resolving the difficulty in achieving uniform mixing due to liquid reduction and gravity in the later stages of filling, as is often the case in existing technologies. Additionally, the device features a suspended storage bag containing cell solution, with the gas located at the top and the filling outlet at the bottom, effectively preventing air bubbles from entering the pipeline during mixing and filling, thus ensuring high filling accuracy.
[0018] Under low temperature conditions, it can fully mix cell preparations, while having advantages such as low shear force, no damage to cells, and high filling accuracy. Attached Figure Description
[0019] Figure 1 This is the structure of the first embodiment of the low-temperature mixing device of this utility model. Figure 1 ;
[0020] Figure 2 This is the structure of the first embodiment of the low-temperature mixing device of this utility model. Figure 2 ;
[0021] Figure 3 This is the structure of the first embodiment of the low-temperature mixing device of this utility model. Figure 3 ;
[0022] Figure 4 This is a schematic diagram of the rollers in different positions in the first embodiment of the low-temperature mixing device of this utility model;
[0023] Figure 5 This is a schematic diagram of the extrusion head assembly structure of this utility model;
[0024] Figure 6 This is the second embodiment of the low-temperature mixing device of this utility model. Figure 1 ;
[0025] Figure 7 This is a schematic diagram of the roller running trajectory in the second embodiment of the low-temperature mixing device of this utility model;
[0026] Figure 8 This is the third embodiment of the low-temperature mixing device of this utility model. Figure 1 ;
[0027] Figure 9 This is the third embodiment of the low-temperature mixing device of this utility model. Figure 2 ;
[0028] Figure 10 This is a schematic diagram of the movement trajectory of the liquid storage bag in the third embodiment of the low-temperature mixing device of this utility model.
[0029] Figure 11 This is a structural diagram of the fourth embodiment of the low-temperature mixing device of this utility model;
[0030] Figure 12 This is a schematic diagram of the outer frame of the fourth embodiment of the low-temperature mixing device of this utility model in the first position;
[0031] Figure 13 This is a schematic diagram of the outer frame of the fourth embodiment of the low-temperature mixing device of this utility model in the second position;
[0032] Figure 14 This is a schematic diagram of the outer frame of the fourth embodiment of the low-temperature mixing device of this utility model in the third position. Detailed Implementation
[0033] See Figure 1-5 This is the first embodiment of the low-temperature mixing device of the present invention. The low-temperature mixing device includes a roller assembly 2, a moving frame 3, a cooling plate 4, an outer frame 5, a temperature detection device (not shown), and an extrusion head assembly 8.
[0034] The cooling plate 4 is located within the outer frame 5. The surface temperature of the cooling plate 4 can be set between 2-8℃, with an accuracy controllable within ±2℃, depending on actual needs. The cooling plate 4 employs a conventional cooling method. (See [link to relevant documentation]). Figure 2 A refrigeration pipe 12 is embedded in the refrigeration plate 4 and is connected to the compressor 13. A heat insulation frame 11 is also arranged between the refrigeration plate 4 and the outer frame 5 to block heat transfer between the refrigeration plate 4 and the outer frame 5.
[0035] The movable frame 3 includes a first support plate 31 and a second support plate 32 arranged opposite to each other. The roller assembly 2 includes a roller 21 and a bearing 22. The bearing 22 passes through the roller 21. One end of the bearing 22 is pivotally connected to the first support plate 31, and the other end of the bearing 22 is locked to the second support plate 32. That is, the other end of the bearing 22 can be fixed to the second support plate 32 as needed, or this fixing can be removed to separate the other end of the bearing 22 from the second support plate 32.
[0036] For example, the other end of the bearing 22 is magnetically locked to the second support plate 32. Preferably, the end of the second support plate 32 is provided with a slot 321, and the other end of the bearing 22 has a first locking hole. At least one side of the slot 321 is provided with a second locking hole corresponding to the first locking hole. When the other end of the bearing is inserted into the slot 321, a pin is used to pass through the first locking hole and the second locking hole to fix the other end of the bearing 22 to the second support plate 32.
[0037] Therefore, the roller assembly 2 can be opened and closed in a manner similar to a door. When open, the liquid storage bag 1 is hung on the cooling plate 4. When closed, the roller 21 is pressed onto the liquid storage bag 1.
[0038] The movable frame 3 can move up and down and back and forth relative to the cooling plate 4, so that the roller 21 continuously squeezes the liquid storage bag 1 to achieve uniform mixing of the internal liquid.
[0039] The extrusion head assembly 8 is disposed at the lower part of the cooling plate 4. The extrusion head assembly 8 includes a plurality of extrusion heads 81, which can extend and retract relative to the outer surface of the cooling plate 4. The heads of the extrusion heads are made of silicone material, which will not damage the liquid storage bag.
[0040] When in use, set the temperature value (e.g., 4℃). The compressor 13 starts running, and the temperature detection device feeds back the temperature information of the cooling plate 4 to the control system in real time. After the temperature reaches the set temperature, the second support plate 32 releases the lock on the bearing 22, and the roller assembly 2 rotates relative to the first support plate 31, moving away from the cooling plate 4. The liquid storage bag 1 containing the cell preparation that needs to be filled at low temperature is installed on the cooling plate 4 (e.g., by using a hook to hang it on the cooling plate), and then the second support plate 32 locks the bearing 22.
[0041] After fixing the outlet pipe 7 to the peristaltic pump and setting parameters such as the filling volume, filling begins. In the early stage of low-temperature mixing and filling, the roller assembly 2 and the moving frame 3 move synchronously up and down and back and forth along the cooling plate 4, thereby squeezing the liquid storage bag 1 and achieving mixing. A layer of silicone is fitted onto the roller 21, which generates friction upon contact with the liquid storage bag 1. The roller 21 moves up and down through its rotation. The moving frame 3 moves up and down and back and forth controlled by a servo motor.
[0042] At the beginning of the filling process, roller 21 and the moving frame 3 are positioned at the bottom of the equipment. Roller 21 is close to the liquid storage bag 1, approximately 40-55mm away from the cooling plate 4 (this distance may vary depending on the liquid volume). Roller 21 and the moving frame 3 move upwards synchronously along the cooling plate 4, compressing the liquid in the liquid storage bag 1. After roller 21 and the moving frame 3 reach the top of the liquid storage bag 1, the servo motor releases the moving frame 3 forward a short distance, away from the cooling plate 4. Roller 21 and the moving frame 3 then move vertically and rapidly downwards to the bottom of the liquid storage bag 1. The servo motor then adjusts the moving frame 3 and roller 21 to move closer to the cooling plate 4, repeating the above trajectory. As filling progresses, the servo motor adjusts the moving frame 3 in real time, shortening the distance between roller 21 and the cooling plate 4.
[0043] By using the driving roller 21 to squeeze and mix the liquid upwards, the problem of uneven cell density caused by cell sedimentation over time during the filling of cell preparations is solved.
[0044] During the later stages of low-temperature mixing and filling, when the distance between roller 21 and cooling plate 4 is approximately 10-20mm, the control system stops the up-and-down movement of roller 21 and moving frame 3. Roller 21 stops at the lower end of liquid storage bag 1, approximately 10-20mm away from cooling plate 4, and is in close contact with liquid storage bag 1. During the mixing process in the later stages of filling, roller 21 serves to fix liquid storage bag 1 in place.
[0045] Subsequently, the control system activates the squeezing head assembly 8, driving multiple squeezing heads 81 to alternately extend and retract, thereby squeezing the liquid storage bag 1, similar to a massage, to mix the liquid inside the bag. The squeezing heads 81 are mushroom-shaped. The heads of the squeezing heads are made of silicone, which will not damage the liquid storage bag.
[0046] See Figure 6-7This is the second embodiment of the low-temperature mixing device of the present invention. The low-temperature mixing device includes a cooling plate 4, an outer frame 5, a temperature detection device, a roller assembly 2, and a rocking arm 75.
[0047] Unlike the first embodiment, the outer frame 5 has elongated grooves extending vertically at both ends. The rocker arm 75 passes through these grooves. The rocker arm 75 includes a first rocker plate 751 and a second rocker plate 752 arranged opposite each other. The roller assembly 2 includes three rollers 21 arranged in an upper, middle, and lower configuration, and three bearings 22 passing through each of the three rollers. Washers 23 are provided on both sides of the three rollers. The bearings of the upper and lower rollers are fixedly connected to the washers 23 at both ends. The bearings of the middle roller pass through the washers 23 on both sides and are fixedly connected to the front ends of the first rocker plate 751 and the second rocker plate 752 respectively. One end of the bearing passing through the middle roller passes through the washer 23 and the threaded hole 753 at the front end of the first rocker plate 751, and the other end passes through the washer 23 and the threaded hole 753 at the front end of the second rocker plate 752, and is fixed with screws.
[0048] The rear ends of the first rocking plate 751 and the second rocking plate 752 are connected to the drive mechanism on the back of the outer frame 5. The drive mechanism can drive the first rocking plate 751 and the second rocking plate 752 to reciprocate around the axis 754, thereby squeezing the liquid in the storage bag 1.
[0049] During the mixing process of the cell preparation, the three rollers 21 significantly increase the contact area with the liquid storage bag 1. When the liquid volume in the storage bag is large, the liquid can be fully squeezed to achieve the purpose of mixing. As the liquid in the storage bag decreases, the servo motor controls the axis 754 of the rocker arm 75 to move away from the cooling plate 4, and then the roller assembly 2 moves closer to the cooling plate 4, repeatedly swinging back and forth to complete the mixing of different volumes.
[0050] The filling process is as follows: Before filling, initialize the mixing equipment on the control panel. The roller assembly 2 and the rocker arm 75 are moved away from the cooling plate 4, with a distance of approximately 80-100mm. The liquid storage bag 1 is suspended on the hook at the top of the cooling plate 4. After suspension, based on the initial volume of the liquid storage bag 1, set the corresponding parameters (e.g., swing start position, swing speed, retraction distance, etc.) and adjust the distance between the roller assembly 2 and the cooling plate 4 so that the roller assembly 2 just contacts the lower end of the liquid storage bag 1. The servo motor drives the rocker arm 75, causing the roller assembly 2 to reciprocate relative to the axis 754 of the rocker arm 75, gradually approaching the cooling plate 4 and squeezing the liquid in the liquid storage bag 1. After reaching the highest point of its trajectory, the roller assembly 2 quickly returns to the lower end of the liquid storage bag 1, repeating the reciprocating swing. As the liquid in the storage bag decreases, the servo motor controls the axis 754 of the rocker arm 75 to move away from the cooling plate 4, causing the roller assembly 2 to move closer to the cooling plate 4, repeating the reciprocating swing trajectory (see...). Figure 7 ), to complete the mixing of different volumes.
[0051] During the later stages of filling, when the distance between the roller assembly 2 and the cooling plate is 10-20mm, the movement of the rocker arm 75 is stopped. The roller 21 stops at the lower end of the liquid storage bag 1, approximately 10-20mm away from the cooling plate 4, and is tightly attached to the liquid storage bag 1 for fixation. Then, the extrusion head assembly 8 is activated, driving multiple extrusion heads 81 to alternately extend and retract, thereby extruding the liquid storage bag 1 and mixing the liquid inside. This extrusion process is the same as in Example 1.
[0052] See Figure 8-10 This is the third embodiment of the low-temperature mixing device of the present invention. The low-temperature mixing device includes a cooling plate 4, an outer frame 5, a temperature detection device, an extrusion head assembly 8, a rack 18, a gear 17, a connecting rod 16, a support plate 14, and a bag pressing mechanism.
[0053] Unlike the first embodiment, it does not use a roller assembly during the mixing process, but instead employs the following structure:
[0054] The pressing mechanism includes left and right swing arms 121 and a pressing plate 122. The two ends of the pressing plate 122 are connected to the lower parts of the left and right swing arms 121, and the upper parts of the swing arms 121 are hinged to the upper left and right sides of the outer frame 5. A torsion spring is sleeved on the hinge shaft, ensuring that the pressing plate 122 always remains pressed against the cooling plate 4. During the mixing process of the cell preparation, the pressing plate 122 presses against the storage bag 1, ensuring that the storage bag 1 remains tightly attached to the cooling plate 4. This prevents the storage bag 1 from detaching from the cooling plate 4 during mixing, which would cause large temperature fluctuations in the storage bag and affect cell quality. Furthermore, a layer of silicone is attached inside the pressing plate 122, preventing damage to the storage bag 1.
[0055] The rack 18 is disposed on the back of the outer frame 5 and extends vertically. Preferably, racks 18 are disposed on both sides of the back of the outer frame 5. A gear 17 is arranged on the support plate 14 and meshes with the rack 18. One end of the connecting rod 16 is hinged to the lower part of the rack 18, and the other end is hinged to the support plate 14. The gear 17 drives the rack 18 to swing up and down by repeatedly rotating forward and backward. At the same time, the movement of the rack 18 and the connecting rod 16 causes the cooling plate 4 and the liquid storage bag 1 to move periodically in an approximately S-shaped trajectory relative to the support plate 14, thereby causing the cells in the liquid storage bag 1 to sway basically along the S-shaped trajectory (see...). Figure 10 ).
[0056] The filling process is as follows: Before filling, open the bag pressing mechanism, move the pressing plate 122 away from the cooling plate 4, install the liquid storage bag 1 in the cooling plate 4, and gently lower the bag pressing mechanism. Fix the liquid outlet pipe 7 on the peristaltic pump, set the filling volume and other parameters, and drive the gear 17 to rotate repeatedly in both directions. The liquid storage bag 1 moves periodically in an approximate S-shaped trajectory under the drive of the cooling plate 4.
[0057] As the liquid decreases, the pressure plate 122 gradually reduces the distance between itself and the cooling plate 4 under the action of the torsion spring. When the distance between the pressure plate 122 and the cooling plate is 10-20mm, the gear 17 stops rotating. Then, the extrusion head assembly 8 is activated, driving multiple extrusion heads 81 to alternately extend and retract, thereby extruding and extruding the liquid storage bag 1, similar to massaging, to mix the liquid inside the storage bag 1. This extrusion process is the same as in Embodiment 1.
[0058] See Figure 11-14 This is the fourth embodiment of the low-temperature mixing device of this utility model. The low-temperature mixing device includes a cooling plate 4, an outer frame 5, a temperature detection device, an extrusion head assembly 8, and a bag pressing mechanism. The structure and operation of the cooling plate 4, outer frame 5, temperature detection device, and extrusion head assembly 8 are basically the same as those in Embodiment 1. The structure and working principle of the bag pressing mechanism will be described in detail below.
[0059] The low-temperature mixing device further includes a support frame 71 and a rocking frame 72. The rocking frame 72 is fixed to the back of the outer frame 5. A bushing is provided on the rocking frame 72, and a corresponding shaft hole is provided on the support frame 71. A shaft 73 passes through the shaft hole and is inserted into the bushing, thereby allowing the rocking frame 72 to pivot relative to the support frame 71. Preferably, the axis of the shaft 73 is parallel to the upper and lower center axes A of the outer frame.
[0060] The top of the outer frame 5 is provided with a first arc-shaped groove 51 and a second arc-shaped groove 52. The first arc-shaped groove 51 and the second arc-shaped groove 52 are respectively located on both sides of the shaft 73, with their centers on the center line of the shaft 73. A first pin 53 is provided in the first arc-shaped groove 51, and a second pin 54 is provided in the second arc-shaped groove 52. The bag-pressing mechanism includes a first connecting rod 91 and a second connecting rod 92. The two ends of the first connecting rod 91 are respectively hinged to the shaft 73 and the first pin 53 (that is, the two ends of the first connecting rod 91 are respectively provided with holes, which are sleeved on the shaft 73 and the first pin 53). The two ends of the second connecting rod 92 are respectively hinged to the shaft 73 and the second pin 54. The first pin 53 and the second pin 54 can slide in the first arc-shaped groove 51 and the second arc-shaped groove 52, respectively.
[0061] The bag-pressing mechanism further includes a third link 93 and a fourth link 94. One end of the third link 93 is hinged to the first pin 53, and the other end is hinged to the upper end of the first roller 931. One end of the fourth link 94 is hinged to the second pin 54, and the other end is hinged to the upper end of the second roller 941. The first roller 931 and the second roller 941 extend vertically along the outer frame 5. The liquid storage bag 1 is sandwiched between the first roller 931, the second roller 941, and the cooling plate 4. The surfaces of the first roller 931 and the second roller 941 are coated with a layer of silicone to prevent damage to the liquid storage bag.
[0062] In order to maintain the smooth operation of the first roller 931 and the second roller 941, a third arc-shaped groove corresponding to the first arc-shaped groove 51 and a fourth arc-shaped groove corresponding to the second arc-shaped groove 52 are symmetrically provided at the bottom of the outer frame 5. The third arc-shaped groove has the same shape as the first arc-shaped groove 51, and the fourth arc-shaped groove has the same shape as the second arc-shaped groove 52.
[0063] Similarly, the bag pressing mechanism is also symmetrically provided with a fifth link, a sixth link, a seventh link, and an eighth link at the bottom of the outer frame 5, which correspond to the first link 91, the second link 92, the third link 93, and the fourth link 94, respectively.
[0064] Similarly, one end of the fifth connecting rod is hinged to shaft 73, and the other end is hinged to the third pin, which is located in the third arc-shaped groove. One end of the seventh connecting rod is hinged to the third pin, and the other end is hinged to the lower end of the first roller 931. One end of the sixth connecting rod is hinged to shaft 73, and the other end is hinged to the fourth pin, which is located in the fourth arc-shaped groove. One end of the eighth connecting rod is hinged to the fourth pin, and the other end is hinged to the lower end of the second roller 941.
[0065] See Figure 12-14 The rocking frame 72 begins to swing under the drive of the drive mechanism. Figure 12 This is a schematic diagram of the rocker 72 in the first position, at which point the outer frame 5 is rotated to the leftmost position. Figure 13 This is a schematic diagram of the rocker 72 in the second position, at which time the outer frame 5 is in the middle position; Figure 14 This is a schematic diagram of the shaking frame 72 in the third position, at which point the outer frame 5 is rotated to the rightmost position. During the shaking process, the first roller 931 and the second roller 941 roll back and forth along the surface of the storage bag, alternately squeezing the cells longitudinally to achieve mixing.
[0066] This invention addresses the need for reagents requiring low-temperature filling by incorporating a cooling plate 4. A reciprocating extrusion device holds the storage bag 1 against the cooling plate 4 while simultaneously extruding the cell-filled bag, ensuring uniform cell distribution and resolving the issue of high shear force and cell damage caused by stirring or other mixing methods in existing technologies. Furthermore, the addition of alternating reciprocating extrusion heads in the later stages of filling facilitates mixing of the cell preparation, overcoming the difficulty in achieving uniform mixing due to liquid reduction and gravity in the later stages of filling, as is common in existing technologies. Additionally, the device features a suspended storage bag containing cell fluid, with the gas located at the top and the filling outlet at the bottom, effectively preventing air bubbles from entering the pipeline during mixing and filling, thus ensuring high filling accuracy.
[0067] This invention offers advantages such as fully mixing cell preparations while maintaining low temperature, low shear force to avoid cell damage, and high filling precision.
[0068] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.
Claims
1. A low-temperature mixing device, characterized in that, It includes a cooling plate (4), an outer frame (5), and a reciprocating extrusion device. The cooling plate (4) is located inside the outer frame (5). The reciprocating extrusion device is used to press the liquid storage bag (1) against the cooling plate (4) and to extrude the liquid storage bag (1).
2. The low-temperature mixing device according to claim 1, characterized in that, The low-temperature mixing device also includes an extrusion head assembly (8), which is disposed at the lower part of the cooling plate (4). The extrusion head assembly (8) includes a plurality of extrusion heads (81), which can extend and retract relative to the outer surface of the cooling plate (4).
3. The low-temperature mixing apparatus according to claim 1 or 2, characterized in that, The reciprocating extrusion device includes: a roller assembly (2) and a moving frame (3). The moving frame (3) includes a first support plate (31) and a second support plate (32) arranged opposite to each other. The roller assembly (2) includes a roller (21) and a bearing (22). The bearing (22) passes through the roller (21). One end of the bearing (22) is pivotally connected to the first support plate (31), and the other end of the bearing (22) is locked to the second support plate (32).
4. The low-temperature mixing device according to claim 3, characterized in that, The movable frame (3) can move up and down and back and forth relative to the cooling plate (4).
5. The low-temperature mixing device according to claim 3, characterized in that, The other end of the bearing (22) is magnetically locked to the second support plate (32).
6. The low-temperature mixing device according to claim 3, characterized in that, The end of the second support plate (32) is provided with a slot (321), and the other end of the bearing (22) is provided with a first locking hole. At least one side of the slot (321) is provided with a second locking hole corresponding to the first locking hole. When the other end of the bearing (22) is inserted into the slot (321), a pin is used to pass through the first locking hole and the second locking hole to lock the other end of the bearing (22) with the second support plate (32).
7. The low-temperature mixing apparatus according to claim 1 or 2, characterized in that, The reciprocating extrusion device includes: a roller assembly (2) and a rocking arm (75). The rocking arm (75) includes a first rocking plate (751) and a second rocking plate (752) arranged opposite to each other. The roller assembly (2) includes three rollers (21) arranged in the upper, middle and lower positions, and three bearings (22) passing through the three rollers respectively. The three rollers are provided with pads (23) on both sides. The bearings of the upper roller and the lower roller are fixedly connected to the pads (23) at both ends. The bearings of the middle roller pass through the pads (23) on both sides and are fixedly connected to the front ends of the first rocking plate (751) and the second rocking plate (752) respectively. The rear ends of the first rocking plate (751) and the second rocking plate (752) are connected to the drive mechanism on the back of the outer frame (5). The drive mechanism can drive the first rocking plate (751) and the second rocking plate (752) to reciprocate around the axis (754).
8. The low-temperature mixing apparatus according to claim 1 or 2, characterized in that, The reciprocating extrusion device includes a bag pressing mechanism, which includes left and right swing arms (121) and a pressure plate (122). The two ends of the pressure plate (122) are connected to the lower parts of the left and right swing arms (121). The upper part of the swing arms (121) is hinged to the upper left and right sides of the outer frame (5). A torsion spring is provided on the hinge shaft so that the pressure plate (122) always presses against the cooling plate (4).
9. The low-temperature mixing apparatus according to claim 8, characterized in that, The reciprocating extrusion device further includes: a rack (18), a gear (17), a connecting rod (16), and a support plate (14). The rack (18) is set on the back of the outer frame (5) and extends up and down. The gear (17) is arranged on the support plate (14) and meshes with the rack (18). One end of the connecting rod (16) is hinged to the lower part of the rack (18) and the other end is hinged to the support plate (14). Driven by the repeated forward and reverse rotation of the gear (17), the cooling plate (4) moves in a roughly S-shaped trajectory relative to the support plate (14) through the movement of the rack (18) and the connecting rod (16).
10. The low-temperature mixing apparatus according to claim 1 or 2, characterized in that, The reciprocating extrusion device includes: a support frame (71), a rocking frame (72), and a bag pressing mechanism. The rocking frame (72) is fixed to the back of the outer frame (5). A bushing is provided on the rocking frame (72), and a corresponding shaft hole is provided on the support frame (71). A shaft (73) passes through the shaft hole and is inserted into the bushing, so that the rocking frame (72) can pivot relative to the support frame (71). A first arc groove (51) and a second arc groove (52) are provided on the top of the outer frame (5). The first arc groove (51) and the second arc groove (52) are located on both sides of the shaft (73). A first pin (53) is provided in the first arc groove (51), and a second pin (54) is provided in the second arc groove (52). The bag pressing mechanism includes a first connecting rod (91) and a second connecting rod (92). The two ends of the first connecting rod (91) are hinged to the shaft (73) and the first pin (53) respectively. The two ends of the second connecting rod (92) are hinged to the shaft (73) and the second pin (54) respectively. The first pin (53) and the second pin (54) can slide in the first arc groove (51) and the second arc groove (52) respectively. The bag pressing mechanism also includes a third link (93) and a fourth link (94). One end of the third link (93) is hinged to the first pin (53), and the other end is hinged to the upper end of the first roller (931). One end of the fourth link (94) is hinged to the second pin (54), and the other end is hinged to the upper end of the second roller (941). The first roller (931) and the second roller (941) extend vertically along the outer frame (5). The liquid storage bag (1) is sandwiched between the first roller (931), the second roller (941) and the cooling plate (4).
11. The low-temperature mixing apparatus according to claim 10, characterized in that, At the bottom of the outer frame (5), a third arc groove corresponding to the first arc groove (51) and a fourth arc groove corresponding to the second arc groove (52) are symmetrically provided. The bag pressing mechanism is also symmetrically provided at the bottom of the outer frame (5) with a fifth link, a sixth link, a seventh link, and an eighth link respectively corresponding to the first link (91), the second link (92), the third link (93), and the fourth link (94). The fifth link is hinged at one end to the shaft (73) and at the other end to the third pin, which is located in the third arc groove. The seventh link is hinged at one end to the third pin and at the other end to the lower end of the first roller (931). The sixth link is hinged at one end to the shaft (73) and at the other end to the fourth pin, which is located in the fourth arc groove. The eighth link is hinged at one end to the fourth pin and at the other end to the lower end of the second roller (941).