Microflora preparation equipment in anaerobic environment

Abstract

The utility model relates to the field of anaerobic preparation equipment, in particular to flora preparation equipment in an anaerobic environment, which comprises an anaerobic glove box, a first gas inlet and outlet structure, a second gas inlet and outlet structure, a separator, a centrifugal machine and a conveying component, the anaerobic glove box comprises an operation compartment and a buffer compartment, the separator and the centrifuge are arranged in the operation compartment, the conveying assembly is arranged in the buffer compartment, and an openable and closable sealing door is arranged between the operation compartment and the buffer compartment; the operation compartment is connected with the first air inlet and outlet structure, and the buffer compartment is connected with the second air inlet and outlet structure. According to the utility model, the first gas inlet and outlet structure and the second gas inlet and outlet structure are arranged to respectively provide anaerobic environments for the operation compartment and the buffer compartment, so that the environmental consistency in the flora extraction and treatment process is ensured, and the influence of external environmental factors on anaerobic flora is reduced; in the operation process, the samples are conveyed into the operation compartment from the buffering compartment through the conveying assembly, the operation can be completed without changing hands, operation is easy, and efficiency is high.

Description

TECHNICAL FIELD

[0001] The utility model relates to anaerobic preparation equipment field, concretely to a kind of flora preparation equipment under anaerobic environment. BACKGROUND

[0002] When flora preparation is carried out, some flora needs to be carried out in anaerobic environment. For example, when intestinal flora transplantation is carried out, since the microbial community in human intestinal tract is mainly composed of anaerobes, it is necessary to be treated in an oxygen-free environment to ensure the activity of these microorganisms. However, in the existing intestinal flora transplantation technology, there is a lack of effective anaerobic environment control means, resulting in a low survival rate of anaerobic flora during transplantation, which affects the effect of transplantation.

[0003] In the invention patent with application publication number CN109109025A, a simple anaerobic operation box is disclosed, which comprises a transparent cuboid-shaped box body, a circular box cover is arranged on the top of the box body, operation holes are arranged on the left and right sides of the front part of the box body, waste liquid tanks are arranged on the left and right sides of the bottom of the box body, a liquid discharge hole is arranged at the connection between the waste liquid tank and the side of the box body, air vents are also arranged on the sides of the box body, and an ultraviolet sterilization lamp is also arranged inside the box body. In this scheme, the anaerobic box only provides an anaerobic flora treatment environment, it is difficult to ensure the consistency of the extraction and treatment environment conditions of the anaerobic flora, and the anaerobic flora in the intestinal contents is easily affected by external bacteria and oxygen, so it is difficult to well guarantee the number and quality of the anaerobic flora. Moreover, if the extraction of intestinal flora is exposed to air, it is difficult to avoid the pollution of the extracted bacteria by the environment.

[0004] In the prior art, there is also an intestinal flora extraction and treatment device arranged in the anaerobic box, and a buffer room connected to one side of the anaerobic box is arranged to ensure the consistency of the extraction and treatment environment conditions of the anaerobic flora and avoid the pollution of the extracted bacteria by the environment. For example, in the invention patent with application publication number CN112111392A, an anaerobic operation box for extracting intestinal flora from feces is disclosed, which comprises a main box body and a buffer room arranged adjacent to each other, the main box body is sealed, and an ethanol combustion device, a temperature detection control device, an oxygen concentration detection module and an ultraviolet sterilization lamp are arranged in the main box body.

[0005] However, since the operation box can only be operated through the glove hole arranged on the box body, in actual operation, the hand close to the sealed door is opposite to the opening direction of the internal box door, so it is difficult to operate, and the hand that is easy to operate is far away from the internal box door, so it is difficult to touch the internal box door and take the articles in the buffer room, and frequent hand changing operation is needed, which is complicated and low in efficiency. UTILITY MODEL CONTENTS

[0006] In order to overcome the problems in the prior art, the utility model aims to provide a flora preparation equipment under anaerobic environment, which can effectively control the anaerobic environment and facilitate operation to improve efficiency.

[0007] To achieve the above object, the utility model provides the following technical scheme: a kind of flora preparation equipment under anaerobic environment, including anaerobic glove box, first air inlet and outlet structure, second air inlet and outlet structure, separation instrument, centrifuge and conveying assembly;

[0008] The anaerobic glove box includes an operating compartment and a buffer compartment, the separation instrument and the centrifuge are arranged in the operating compartment, and the conveying assembly is arranged in the buffer compartment.

[0009] The utility model further provides that the conveying assembly includes a placement table, a driving device and a linear reciprocating structure.

[0010] In actual use, the linear reciprocating structure is driven by the driving device to move, and the placement table is extended into the operating compartment or withdrawn from the sealing door.

[0011] The utility model further provides that the linear reciprocating structure is a conveyor belt structure, which includes a driving gear, a driven gear and a conveyor belt.

[0012] The utility model further provides that the inner wall of the operating compartment away from the buffer compartment is provided with a fixing frame, the separation instrument is arranged on the fixing frame, and the centrifuge is arranged below the fixing frame.

[0013] The conveyor belt structure conveys the placement table from the sealing door to the operating compartment, after taking the sample on the placement table, the conveyor belt structure drives the placement table to withdraw into the buffer compartment, and then the sample is processed on the operating table or extracted by the separation instrument, the centrifuge and the like.

[0014] The utility model further provides that the operating compartment is provided with a plurality of operation holes, and gloves are sealingly arranged at the operation holes.

[0015] The operation holes are arranged near the operating table, the separation instrument and the centrifuge, which facilitates operation.

[0016] The utility model further provides that the operating compartment is further provided with a door opening motor, and the door opening motor is connected with the sealing door.

[0017] The existing anaerobic box is difficult to operate when the hand close to the sealing door is opposite to the opening direction of the sealing door, and the hand far from the sealing door is difficult to touch the sealing door and take the objects in the buffer room.

[0018] The utility model further sets up: still be provided with disinfection device in the operation room, disinfection device is ultraviolet disinfection module or epoxy methane disinfection module.

[0019] The disinfection device can disinfect the whole system before and after use to avoid cross contamination.

[0020] The utility model further sets up: still be provided with temperature control system and humidity adjusting device in the operation room.

[0021] The temperature control system ensures that the temperature in the glove box is maintained within the range suitable for microbial growth. The humidity adjusting device can maintain appropriate humidity level in the glove box to prevent the bacterial solution from drying.

[0022] The utility model further sets up: first air inlet and first air outlet, first air inlet is provided with first air inlet valve, first air outlet is provided with first air outlet valve,

[0023] Second air inlet and second air outlet, second air inlet is provided with second air inlet valve, second air outlet is provided with second air outlet valve,

[0024] The operation room and the buffer room are respectively provided with an oxygen content detector.

[0025] The utility model further sets up: the bacterial population preparation equipment under anaerobic environment still includes control module, control module is electrically connected with drive arrangement, door motor, disinfection device, temperature control system, humidity adjusting device, first air inlet valve, first air outlet valve, second air inlet valve, second air outlet valve and oxygen content detector.

[0026] In summary, the beneficial effects of the above technical solutions of the utility model are as follows:

[0027] 1. The utility model discloses a first air inlet and a second air inlet are arranged in the operation compartment and the buffer compartment respectively, the effect that inert gas replaces the air in the sealed glove box is adopted, a strict anaerobic environment is provided, the external air and bacteria are isolated, the consistency of the environment in the process of bacterial population extraction and processing is guaranteed, the influence of external environmental factors on anaerobic bacterial population is reduced, and the quantity and quality of the processed anaerobic bacterial population are guaranteed. The survival rate of anaerobic bacterial population in the transplantation process is significantly improved.

[0028] 2. The utility model discloses a door opening motor can automatically open the sealing door, and then the conveying assembly conveys the placing table from the sealing door to the operation compartment, which can be completed without changing hands, and is simple and efficient to operate.

[0029] 3. The utility model discloses efficient disinfection device and isolation measures are arranged, and the risk of cross contamination is reduced.

[0030] 4. The utility model discloses temperature control system and humidity adjusting device ensure that the temperature and humidity in the glove box are kept in the range suitable for microorganism growth, and the survival rate of anaerobic bacterial population in the transplantation process is significantly improved. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the following will be briefly introduced the drawings needed to be used in the embodiment, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creating creative labor.

[0032] Fig. 1 It is the internal structure schematic diagram of bacterial population preparation equipment.

[0033] Fig. 2 It is the main view of bacterial population preparation equipment of conveying assembly driving placing table and extending into operation compartment state.

[0034] Fig. 3 It is the main view of bacterial population preparation equipment of conveying assembly driving placing table and extending into operation compartment state.

[0035] In the drawings, the component list represented by each sign is as follows:

[0036] 100, operation compartment, 101, operation hole, 102, operation table, 103, first air inlet, 104, first air outlet;

[0037] 200, buffer compartment, 201, buffer compartment door, 202, second air inlet, 203, second air outlet;

[0038] 300, conveying assembly, 301 placing table, 302, fixed block, 303, driving device.

[0039] 400. Sealed door; 401. Door opening motor;

[0040] 500. Separator; 600. Centrifuge; 700. Sterilization device; 800. Humidity regulator; 810. Temperature control system; 900. Control module. Detailed Implementation

[0041] To enable those skilled in the art to better understand the technical solution of this utility model, the technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. Based on the embodiments of this utility model, other similar embodiments obtained by those skilled in the art without creative effort should all fall within the protection scope of this utility model. Furthermore, directional terms mentioned in the following embodiments, such as "up," "down," "left," and "right," are only for reference to the directions in the accompanying drawings; therefore, the directional terms used are for illustrative purposes and not for limiting the scope of this utility model.

[0042] The present invention will be further described below with reference to the accompanying drawings and preferred embodiments.

[0043] Example:

[0044] like Figs. 1-3 As shown, this is a preferred embodiment of the present invention, a microbial community preparation device under anaerobic environment, including an anaerobic glove box, a first inlet and outlet structure, a second inlet and outlet structure, a separator 500, a centrifuge 600 and a conveying component 300.

[0045] The anaerobic glove box includes an operating compartment 100 and a buffer compartment 200. The separator 500 and centrifuge 600 are located in the operating compartment 100, and the conveying assembly 300 is located in the buffer compartment 200. An openable and closable sealing door 400 is provided between the operating compartment 100 and the buffer compartment 200. The position of the conveying assembly 300 matches the position of the sealing door 400. The operating compartment 100 is connected to a first inlet / outlet structure, and the buffer compartment is connected to a second inlet / outlet structure.

[0046] The conveying assembly 300 includes a placement platform 301, a drive device 303, and a linear reciprocating structure. The placement platform 301 is fixed on the linear reciprocating structure, and the drive device 303 is connected to the linear reciprocating structure. In actual use, the drive device 303 drives the linear reciprocating structure to extend the placement platform 301 into the operating compartment 100 or retract it from the sealing door 400.

[0047] In this embodiment, the straight reciprocating structure is a conveyor belt structure, which comprises a driving gear, a driven gear and a conveyor belt, one end of the conveyor belt is fixedly connected to the driving gear, and the other end is fixedly connected to the driven gear; the driving gear is connected to the driving device 303, and the bottom of the end of the placing table 301 away from the sealing door is provided with a fixed block 302, which is fixed on the conveyor belt.

[0048] The operating compartment 100 is also provided with an opening door motor 401 connected to the sealing door 400.

[0049] The operating compartment 100 is provided with a fixed frame on the inner wall away from the buffer compartment 200, and the separation instrument 500 is arranged on the fixed frame, and the centrifuge 600 is arranged below the fixed frame.

[0050] The conveyor belt structure transports the placing table 301 from the sealing door 400 to the operating compartment 100, and after taking the sample on the placing table 301, the conveyor belt structure drives the placing table 301 to return to the buffer compartment 200, and then the sample is processed on the operating table 102 or the separation instrument 500 and the centrifuge 600 are used for extracting intestinal bacteria.

[0051] The operating compartment 100 is provided with a plurality of operation holes 101, and gloves are arranged on the operation holes 101. The operation holes 101 are arranged near the operating table 102, the separation instrument 500 and the centrifuge 600, which is convenient for operation. The buffer compartment 200 is also provided with a buffer compartment door 201 for placing and taking the sample in the buffer compartment 200.

[0052] The operating compartment 100 is also provided with a disinfection device 700, which is an ultraviolet disinfection module or an ethylene oxide disinfection module.

[0053] The disinfection device 700 can disinfect the whole system before and after use to avoid cross contamination.

[0054] The operating compartment 100 is also provided with a temperature control system 810 and a humidity adjusting device 800. The temperature control system 810 ensures that the temperature in the operating compartment 100 is maintained within the range suitable for microbial growth. The humidity adjusting device can maintain the appropriate humidity level in the operating compartment 100 to prevent the bacterial solution from drying.

[0055] The first air inlet structure comprises a first air inlet 103 and a first air outlet 104; the first air inlet 103 is provided with a first air inlet valve, and the first air outlet 104 is provided with a first air outlet valve;

[0056] The second air inlet structure comprises a second air inlet 202 and a second air outlet 203; the second air inlet 202 is provided with a second air inlet valve, and the second air outlet 203 is provided with a second air outlet valve; the operating compartment 100 and the buffer compartment 200 are respectively provided with oxygen content detectors.

[0057] The first air inlet 103 and the second air inlet 202 are connected with external gas conveying devices, inert gas is injected into the operating compartment 100 and the buffer compartment 200 through the first air inlet 103 and the second air inlet 202, and the internal oxygen is replaced. The replaced gas is discharged from the first air outlet 104 and the second air outlet 203.

[0058] The anaerobic environment bacteria colony preparation device further comprises a control module 900, which is electrically connected with the driving device 303, the door opening motor 401, the sterilization device 700, the temperature control system 810, the humidity adjusting device 800, the first air inlet valve, the first air outlet valve, the second air inlet valve, the second air outlet valve and the oxygen content detectors.

[0059] In actual operation, first, the sterilization device 700 is started to sterilize the operating compartment 100, the sealing door 400 is closed to ensure that the operating compartment 100 is not in contact with external air, the first air inlet valve and the first air outlet valve are opened by the control module 900, so that the operating compartment 100 becomes an anaerobic environment. The buffer compartment door 201 is opened, the sample is placed on the placement table 301, and then the buffer compartment door 201 is closed, the second air inlet valve and the second air outlet valve are opened by the control module 900, and the oxygen content detectors in the two compartments are compared, so that the buffer compartment 200 becomes an anaerobic environment as the operating compartment 100. The sealing door 400 is opened by the control module 900 controlling the door opening motor 401, and the driving device 303 is started to convey the sample into the operating compartment 100, the operator puts his hand into the glove in the operation hole 101 of the operating compartment 100 to prepare the sample and the like, the centrifuge 600 is used to separate the bacteria and the culture solution, so as to facilitate subsequent analysis or processing, and the separator 500 is used to separate specific bacteria through stirring and fractional filtration. The temperature control system 810 and the humidity adjusting device 800 are adjusted to keep the operating compartment 100 within a range suitable for microbial growth.

[0060] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A device for preparing microbial communities under anaerobic conditions, characterized in that, The anaerobic glove box, the first gas inlet and outlet structure, the second gas inlet and outlet structure, the separation instrument, the centrifuge and the conveying assembly are included. The anaerobic glove box includes an operation compartment and a buffer compartment, the separation instrument and the centrifuge are arranged in the operation compartment, the conveying assembly is arranged in the buffer compartment, a sealable door is arranged between the operation compartment and the buffer compartment, the position of the conveying assembly is matched with the position of the sealable door, the operation compartment is connected with the first gas inlet and outlet structure, and the buffer compartment is connected with the second gas inlet and outlet structure.

2. The apparatus according to claim 1, wherein The conveying assembly includes a placing table, a driving device and a linear reciprocating structure, the placing table is fixed on the linear reciprocating structure, and the driving device is connected with the linear reciprocating structure.

3. The apparatus according to claim 2, wherein The linear reciprocating structure is a conveyor belt structure, the conveyor belt structure includes a driving gear, a driven gear and a conveying belt, one end of the conveying belt is fixed on the driving gear in tension, and the other end of the conveying belt is fixed on the driven gear. The driving gear is connected with the driving device, and a fixed block is arranged at the bottom of the end of the placing table away from the sealable door, and the fixed block is fixed on the conveying belt.

4. The apparatus according to claim 1, wherein A fixed frame is arranged on the inner wall of the operation compartment away from the buffer compartment, the separation instrument is arranged on the fixed frame, the centrifuge is arranged below the fixed frame, and an operation table is arranged between the centrifuge and the buffer compartment.

5. The apparatus according to claim 1, wherein The operation compartment is provided with a plurality of operation holes, and gloves are arranged at the operation holes in a sealed mode.

6. The apparatus according to claim 3, wherein An opening door motor is further arranged in the operation compartment, and the opening door motor is connected with the sealable door.

7. The apparatus according to claim 6, wherein A disinfection device is further arranged in the operation compartment, and the disinfection device is an ultraviolet disinfection module or an ethylene oxide disinfection module.

8. The apparatus according to claim 7, wherein A temperature control system and a humidity adjusting device are further arranged in the operation compartment.

9. The apparatus according to claim 8, wherein The first gas inlet and outlet structure includes a first gas inlet and a first gas outlet, a first gas inlet valve is arranged at the first gas inlet, and a first gas outlet valve is arranged at the first gas outlet, the second gas inlet and outlet structure includes a second gas inlet and a second gas outlet, a second gas inlet valve is arranged at the second gas inlet, and a second gas outlet valve is arranged at the second gas outlet. Oxygen content detectors are arranged in the operation compartment and the buffer compartment.

10. The apparatus according to claim 9, wherein The anaerobic environment bacteria preparation equipment further includes a control module, and the control module is electrically connected with the driving device, the opening door motor, the disinfection device, the temperature control system, the humidity adjusting device, the first gas inlet valve, the first gas outlet valve, the second gas inlet valve, the second gas outlet valve and the oxygen content detectors.

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