Liquid nitrogen freezing sample rack for DNA extraction of tobacco production raw materials

Abstract

The utility model relates to the technical field of biological sample treatment, in particular to a liquid nitrogen freezing sample holder for DNA extraction of tobacco-making raw materials, which comprises a sample holder, a counterweight device and a liquid nitrogen flow guide structure, an upper cover plate is further mounted above the sample holder, a lower cover plate is further mounted below the sample holder, a circular bulge is arranged on the upper cover plate, and the lower cover plate is arranged on the lower cover plate. The counterweight device is detachably fixed on the upper cover plate, the liquid nitrogen flow guide structure comprises uniformly distributed flow guide holes, and an EP pipe hole site is arranged in the sample holder; the sample rack, the upper cover plate and the lower cover plate are made of aviation aluminum or 304 stainless steel and are resistant to liquid nitrogen corrosion, the service life is prolonged by 3-5 times, and the equipment maintenance cost is reduced; and the solid aluminum alloy counterweight device is fixed to the upper cover plate in a buckled mode through a circular protrusion, the overall density reaches 1.2 g / cm, it is ensured that the counterweight device is completely immersed in liquid nitrogen, manual pressing is not needed, and the operation safety is improved by 80%.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological sample processing technical field, concretely is a kind of tobacco raw material DNA extraction liquid nitrogen frozen sample rack. BACKGROUND

[0002] Tobacco raw material DNA molecular marker identification test is mainly applied to the quantitative and qualitative identification of smoke and non-smoke, type, variety, etc. of test material. High-quality test material genomic DNA is a prerequisite for ensuring the stability and accuracy of the above identification test results. Currently, the extraction of most plant genomic DNA requires grinding the tissue with liquid nitrogen. On the one hand, low temperature can cause tissue freeze cracking, facilitating grinding to break cells and completely releasing DNA. On the other hand, the low-temperature environment of liquid nitrogen can reduce the activity of DNase, preventing genomic DNA from being degraded during extraction, thereby improving the yield of DNA. Therefore, liquid nitrogen frozen material is an important pretreatment process for tobacco raw material DNA molecular marker identification.

[0003] With the popularization of automated cell disruptors, the mortar liquid nitrogen grinding pretreatment process is gradually replaced. Only a small amount of test material tissue is needed to be placed in a thick-walled ep tube, and then the ep tube is placed under the liquid surface of liquid nitrogen after the grinding beads are put in and the ep tube cover is closed. The ep is then loaded into the sample rack of the automated cell disruptor for grinding. However, due to the buoyancy of liquid nitrogen, the test material tissue often floats on the liquid surface, making it difficult to freeze the test material tissue. Manual assistance is needed to insert the ep into the liquid surface, which poses a risk of liquid nitrogen splash injury to the operator. In addition, the sample rack module of the automated cell disruptor is made of plastic, which prohibits liquid nitrogen freezing. Placing the sample into the sample rack and then into liquid nitrogen can easily damage the sample rack, and the replacement cost is high.

[0004] Therefore, there is a need to design a tobacco raw material DNA extraction liquid nitrogen frozen sample rack to solve the above problems. UTILITY MODEL CONTENTS

[0005] The utility model aims to provide a kind of tobacco raw material DNA extraction liquid nitrogen frozen sample rack to solve the problems raised in the above background.

[0006] To achieve the above purpose, the utility model provides the following technical scheme:

[0007] A kind of tobacco raw material DNA extraction liquid nitrogen frozen sample rack, including sample rack, counterweight device and liquid nitrogen flow guide structure, the upper portion of the sample rack is also provided with upper cover plate, the lower portion of the sample rack is also provided with lower cover plate, the upper cover plate is provided with circular protrusion, the counterweight device can be detachably fixed on the upper cover plate, the liquid nitrogen flow guide structure includes uniformly distributed flow guide hole, the inside of the sample rack is provided with EP tube hole position.

[0008] As the preferred scheme of the utility model, the sample rack, the upper cover plate and the lower cover plate adopt metal material, specifically one of aviation aluminum and 304 stainless steel.

[0009] As the preferred scheme of the utility model, the counterweight device adopts solid aluminum alloy block, is equipped with the circular convex structure matched with the upper cover plate at the bottom, and is fixed through the buckle.

[0010] As the preferred scheme of the utility model, the surface of the counterweight device is coated with polytetrafluoroethylene coating.

[0011] As the preferred scheme of the utility model, the diameter of the flow guide hole on the liquid nitrogen flow guide structure is 2-5mm, and is arranged according to matrix.

[0012] As the preferred scheme of the utility model, the flow guide hole in the liquid nitrogen flow guide structure is funnel-shaped, large at the top and small at the bottom.

[0013] As the preferred scheme of the utility model, the edge of the sample rack is further provided with splash-proof protection structure, and the splash-proof protection structure comprises silica gel sealing ring and double-layer heat insulation cavity.

[0014] As the preferred scheme of the utility model, the silica gel sealing ring is detachable structure.

[0015] As the preferred scheme of the utility model, the inner layer of the double-layer heat insulation cavity adopts foam material with good heat insulation performance, and the outer layer adopts high-strength plastic material.

[0016] As the preferred scheme of the utility model, the inner wall of the EP pipe hole position is provided with anti-skid lines.

[0017] As the preferred scheme of the utility model, the surface of the sample rack, the upper cover plate and the lower cover plate is subjected to passivation treatment.

[0018] Compared with the prior art, the utility model has the beneficial effects that:

[0019] The utility model discloses a kind of tobacco raw material DNA extraction liquid nitrogen frozen sample racks, can realize following effect:1.Material optimization: sample rack, upper cover plate, lower cover plate adopt aviation aluminum or 304 stainless steel, liquid nitrogen corrosion resistance, life extension 3-5 times, reduce equipment maintenance cost;2.Balast stable immersion: solid aluminum alloy counterweight device is fixed by round protrusion and upper cover plate buckle, overall density reaches 1.2g / cm, ensure completely immersed liquid nitrogen, without manual pressing, operation safety improves 80%;3.Liquid nitrogen penetration efficiency improves: flow guide hole makes liquid nitrogen penetration speed accelerate 40%, freezing uniformity improves, reduces DNA degradation risk;4.Splash safety design: splash protection structure includes silica gel sealing ring and double-layer heat insulation cavity, can block liquid nitrogen splash, operation safety improves 90%;5.Equipment compatibility enhances: counterweight device bottom round protrusion is matched with automated equipment positioning hole, without modification directly use;Polytetrafluoroethylene coating reduces metal contact abrasion, prolongs equipment life;6.Operation efficiency improves: 24 tubes can be handled simultaneously, cooperate with automated equipment, grinding efficiency improves 5 times, meet high-throughput detection demand. BRIEF DESCRIPTION OF DRAWINGS

[0020] Fig. 1 It is whole three-dimensional structure schematic diagram of the utility model;

[0021] Fig. 2 It is internal structure schematic diagram of the utility model;

[0022] Fig. 3 It is side view structure schematic diagram of the utility model.

[0023] In the drawing: 1, sample rack;2, upper cover plate;3, lower cover plate;4, round protrusion;5, liquid nitrogen flow guide structure;6, EP tube hole site;7, splash protection structure. DETAILED DESCRIPTION

[0024] The technical scheme in the embodiments of the utility model will be described below in conjunction with the embodiments of the utility model, apparently, the described embodiments are only part of the embodiments of the utility model, not all the embodiments, based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without making creative labor belong to the range of protection of the utility model.

[0025] In order to facilitate understanding of the utility model, the utility model will be described more fully below with reference to relevant drawings. Several embodiments of the utility model are given. However, the utility model can be realized in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and comprehensive.

[0026] It should be noted that when an element is referred to as being "on" another element, it can be directly on the other element or intervening elements can also be present. When an element is referred to as being "connected" or "coupled" to another element, it can be directly connected or coupled to the other element or intervening elements can also be present. As used herein the terms "vertical", "horizontal", "left", "right" and similar terms are used for explanation purposes only.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0028] Embodiments, please refer to Figs. 1-3 The application provides a technical scheme:

[0029] A tobacco raw material DNA extraction liquid nitrogen freezing sample rack, comprising a sample rack 1, a counterweight device and a liquid nitrogen flow guide structure 5, an upper cover plate 2 is further installed above the sample rack 1, and a lower cover plate 3 is further installed below the sample rack 1, the surfaces of the sample rack 1, the upper cover plate 2 and the lower cover plate 3 are all subjected to passivation treatment, a circular protrusion 4 is arranged on the upper cover plate 2, the counterweight device is detachably fixed on the upper cover plate 2, the liquid nitrogen flow guide structure 5 comprises uniformly distributed flow guide holes, an EP tube hole position 6 is arranged in the inside of the sample rack 1, and an anti-skid pattern is arranged on the inner wall of the EP tube hole position; the upper cover plate 2 is installed above the sample rack 1 to ensure stable installation. Then, the lower cover plate 3 is installed below the sample rack 1 to complete the assembly of the sample rack main body. Then, the solid aluminum alloy counterweight device with a circular protrusion structure matched with the upper cover plate 2 is fixed on the upper cover plate 2 through buckling. Then, it is checked whether the EP tube hole position 6 in the inside of the sample rack 1 is normal to ensure that there is no blockage or damage. At the same time, it is confirmed that there is no foreign matter in the uniformly distributed flow guide holes of the liquid nitrogen flow guide structure 5. Finally, the splash-proof protection structure 7 at the edge of the sample rack 1 is checked to ensure that the silica gel sealing ring and the double-layer heat insulation cavity are intact.

[0030] Specifically, the sample holder 1, the upper cover plate 2 and the lower cover plate 3 are made of metal material, specifically one of aviation aluminum and 304 stainless steel; according to actual demand and cost consideration, aviation aluminum or 304 stainless steel is selected to make the sample holder 1, the upper cover plate 2 and the lower cover plate 3. During the manufacturing process, the parts are strictly processed according to the design specifications to ensure the dimensional accuracy and quality of each part. After manufacturing, quality detection is performed on each part to check for defects or damage; aviation aluminum or 304 stainless steel has good corrosion resistance and can resist the low-temperature corrosion of liquid nitrogen, prolonging the service life of the sample holder, reducing the frequency of replacing the sample holder due to material damage, and reducing experimental costs. At the same time, these two materials have high strength, ensuring the structural stability of the sample holder during use and reducing deformation, providing reliable protection for the smooth progress of the experiment.

[0031] Specifically, the counterweight device is made of solid aluminum alloy block, the bottom is provided with a circular protruding structure matched with the upper cover plate 2, and is fixed by buckle; the surface of the counterweight device is coated with polytetrafluoroethylene coating; the solid aluminum alloy counterweight device with circular protruding structure matched with the upper cover plate 2 is manufactured. During installation, the circular protrusion of the counterweight device is aligned with the circular protrusion 4 on the upper cover plate 2, and the two are firmly fixed by buckle. After installation, check whether the buckle is tightened to ensure that the counterweight device will not loosen during use; the design of the counterweight device increases the overall weight of the sample holder and lowers the center of gravity. The circular protruding structure at the bottom matches the design of the upper cover plate 2, which not only facilitates installation, but also ensures the stability of the counterweight device after installation. The buckle fixing method further enhances the reliability of the connection, so that the sample holder can sink stably in liquid nitrogen, avoiding floating on the surface of liquid nitrogen due to the lightness of the sample holder, ensuring that the tobacco raw materials can be fully frozen, and improving the success rate of DNA extraction. In addition, the polytetrafluoroethylene coating on the surface of the counterweight device has good corrosion resistance and low friction coefficient, which can prevent the counterweight device from being corroded by liquid nitrogen, while reducing friction with other objects during operation, avoiding damage to the sample holder and other equipment.

[0032] Specifically, the diameter of the guide holes on the liquid nitrogen guide structure 5 is 2-5 mm, arranged in a matrix; the guide holes in the liquid nitrogen guide structure are funnel-shaped, large at the top and small at the bottom; when manufacturing the liquid nitrogen guide structure 5, according to the design requirements, uniformly open the guide holes with a diameter of 2-5 mm on the structure, and arrange them in a matrix. After completion, check whether the guide holes are unobstructed and whether there is any blockage. When using the sample holder, place the sample holder in liquid nitrogen, and the liquid nitrogen will quickly and uniformly penetrate into the sample holder through these guide holes and fully contact the EP tube placed in the EP tube hole site 6; the design of the guide holes on the liquid nitrogen guide structure 5 greatly improves the guide efficiency of liquid nitrogen. The matrix-arranged guide holes can evenly distribute liquid nitrogen inside the sample holder, ensuring that each EP tube can fully contact liquid nitrogen, achieving rapid freezing and ensuring consistent freezing effect of tobacco raw materials, which is conducive to improving the quality and efficiency of DNA extraction. The smaller guide hole diameter of 2-5 mm can prevent the rapid loss of liquid nitrogen, while preventing impurities from entering the sample holder and affecting the experimental results.

[0033] Specifically, the edge of the sample holder 1 is also provided with a splash-proof protection structure 7, which includes a silica gel sealing ring and a double-layer heat insulation cavity; the silica gel sealing ring is a detachable structure; the inner layer of the double-layer heat insulation cavity is made of foam material with good heat insulation performance, and the outer layer is made of high-strength plastic material; install the splash-proof protection structure 7 at the edge of the sample holder 1, install the silica gel sealing ring at the appropriate position to ensure good sealing, and then install the double-layer heat insulation cavity. When using the sample holder, place it in liquid nitrogen and closely observe the state of the splash-proof protection structure 7 during operation; the silica gel sealing ring in the splash-proof protection structure 7 can effectively seal the edge of the sample holder to prevent liquid nitrogen from splashing out of the edge. The double-layer heat insulation cavity further enhances the protection effect, not only blocking the low temperature of liquid nitrogen, but also reducing the risk of injury to the operator caused by liquid nitrogen splashing out, ensuring the personal safety of the operator. At the same time, the double-layer heat insulation cavity can also reduce the heat exchange between the sample holder and the external environment, which helps to maintain the low-temperature environment inside the sample holder and improve the freezing effect.

[0034] The utility model discloses a work flow: when using the tobacco raw material DNA extraction liquid nitrogen freezing sample holder, first check sample holder 1, ensure that its inside EP pipe hole position 6 is without blockage, is without damage, and sample holder 1 whole structure is complete, is without deformation, crack and other defects;Check upper cover plate 2, confirm that its surface round convex 4 is intact, is not worn or deformed, so as to avoid affecting the connection with the counterweight device;View lower cover plate 3, ensure that its structure is complete, can be closely matched with sample holder 1;Check counterweight device, confirm that its bottom and the round convex structure matched with upper cover plate 2 are normal, and the buckle connecting part is not damaged, and the surface polytetrafluoroethylene coating is complete;Observe liquid nitrogen flow guide structure 5, ensure that its uniformly distributed flow guide hole is not blocked by foreign matter, and the flow guide hole diameter is in 2-5mm range, and the matrix arrangement is normal;Check the splashproof protection structure 7 of sample holder 1 edge, ensure that the silica gel sealing ring is sealed well, and the double-layer heat insulation cavity is not damaged, is not cracked;Then install upper cover plate 2 above sample holder 1, make both accurate alignment, ensure firm installation;Install lower cover plate 3 below sample holder 1, complete the assembly of sample holder main body, ensure that the whole structure is stable;The solid aluminum alloy counterweight device with matched round convex structure is fixed on upper cover plate 2 through buckle, make the round convex of counterweight device closely cooperate with the round convex 4 of upper cover plate 2, check whether the buckle is buckled, ensure that the counterweight device is installed stably;EP tube loaded with tobacco raw material is placed in EP tube hole position 6 in the inside of sample holder 1 in sequence, ensure that the EP tube is placed in place, and will not shake or tilt;Slowly put the sample holder with assembled sample into liquid nitrogen, in the process of putting, liquid nitrogen will quickly and uniformly penetrate into the inside of sample holder through the flow guide hole on liquid nitrogen flow guide structure 5, and fully contact with EP tube, and the tobacco raw material is frozen;In the whole freezing process, the silica gel sealing ring in splashproof protection structure 7 of sample holder 1 edge and double-layer heat insulation cavity play a role, prevent liquid nitrogen from splashing out, protect the safety of operator, reduce the heat exchange between sample holder and external environment, and maintain the internal low-temperature environment;When the tobacco raw material is frozen, carefully take out the sample holder from liquid nitrogen, in the process of taking out, splashproof protection structure 7 continues to prevent liquid nitrogen from splashing out, and ensures the operation safety;After taking out, according to the subsequent experimental requirements, the EP tube can be taken out from the EP tube hole position 6 of sample holder 1, and the next step of DNA extraction and other experimental operations are carried out.

[0035] Although the embodiments of the utility model have been shown and described, it is to be understood that various changes, modifications, substitutions and variations can be made thereto without departing from the principles and spirit of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A kind of tobacco raw material DNA extraction liquid nitrogen frozen sample holder, including sample holder (1), counterweight device and liquid nitrogen flow guide structure (5), it is characterized in that: The upper part of the sample holder (1) is further provided with an upper cover plate (2), and the lower part of the sample holder (1) is further provided with a lower cover plate (3), the upper cover plate (2) is provided with a circular protrusion (4), the counterweight device is detachably fixed on the upper cover plate (2), the liquid nitrogen flow guide structure (5) comprises uniformly distributed flow guide holes, and the inside of the sample holder (1) is provided with an EP tube hole position (6).

2. The liquid nitrogen frozen sample rack for extracting DNA of tobacco raw material according to claim 1, characterized in that: The sample holder (1), the upper cover plate (2) and the lower cover plate (3) are made of metal material, and specifically can be one of aviation aluminum and 304 stainless steel.

3. The liquid nitrogen frozen sample rack for extracting DNA of tobacco raw material according to claim 1, characterized in that: The counterweight device is made of solid aluminum alloy block, and the bottom is provided with a circular protrusion structure matched with the upper cover plate (2) and is fixed by buckling.

4. The liquid nitrogen frozen sample rack for extracting DNA of tobacco raw material according to claim 1, characterized in that: The surface of the counterweight device is coated with a polytetrafluoroethylene coating.

5. The liquid nitrogen frozen sample holder for extracting DNA of tobacco raw material according to claim 1, characterized in that: The diameter of the flow guide holes on the liquid nitrogen flow guide structure (5) is 2-5mm, and the flow guide holes are arranged in a matrix.

6. The liquid nitrogen frozen sample holder for extracting DNA of tobacco raw material according to claim 1, characterized in that: The flow guide holes in the liquid nitrogen flow guide structure (5) are funnel-shaped, large at the top and small at the bottom.

7. The liquid nitrogen frozen sample holder for extracting DNA of tobacco raw material according to claim 1, characterized in that: The edge of the sample holder (1) is further provided with a splash-proof protection structure (7), and the splash-proof protection structure (7) comprises a silica gel sealing ring and a double-layer heat insulation cavity.

8. The liquid nitrogen frozen sample rack for extracting DNA of tobacco raw material according to claim 7, characterized in that: The silica gel sealing ring is a detachable structure.

9. The liquid nitrogen frozen sample holder for extracting DNA of tobacco raw material according to claim 7, characterized in that: The inner layer of the double-layer heat insulation cavity is made of foam material with good heat insulation performance, and the outer layer is made of high-strength plastic material.

10. The liquid nitrogen frozen sample holder for extracting DNA of tobacco raw material according to claim 1, characterized in that: The inner wall of the EP tube hole position (6) is provided with anti-skid lines.

11. The liquid nitrogen frozen sample holder for extracting DNA of tobacco raw material according to claim 1, characterized in that: The surfaces of the sample holder (1), the upper cover plate (2) and the lower cover plate (3) are all passivated.

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