Gradient density liquid automatic layering centrifugal tube for ultracentrifugation of cell exosome

Abstract

The gradient density liquid automatic layering centrifugal tube comprises a tube body and a tube cap, external threads are arranged at the upper end of the tube body, the tube cap is screwed on the external threads at the upper end of the tube body and matched with the upper end face of the tube body in a sealing mode, an air inlet and a sampling opening are formed in the tube body, and the sampling opening is communicated with the tube body. The utility model relates to the technical field of centrifugal tubes, in the ultra-speed centrifugal operation process, the air guide communication component and the sealed sampling component are both in a closed state, a sample and gradient density liquid in the centrifugal tube are automatically layered under the action of centrifugal force, and the gradient density liquid in the centrifugal tube is separated from the sample through the sealed sampling component. When the separation liquid needs to be extracted, the pipe cap does not need to be opened, only the gas guide communication component needs to be opened, the sealing sampling component cover plate needs to be opened, the sampling pipe is inserted into the guide pipe, the sampling pipe further penetrates through the elastic sealing piece to stretch into the separation liquid, and the separation liquid is sampled, so that the operation is convenient, and the sealing performance is good.

Description

Technical Field

[0001] This utility model relates to the field of centrifuge tube technology, specifically to an automatic stratification centrifuge tube for ultracentrifugation of cell exosomes using gradient density liquid. Background Technology

[0002] Ultracentrifugation (UC) is a classic method for exosome extraction. Through centrifugation at different speeds, cells, cell debris, and macromolecules are gradually removed, ultimately yielding an exosome precipitate. This method is mainly divided into differential ultracentrifugation (DUC) and density gradient ultracentrifugation. Density gradient ultracentrifugation separates samples by adding an inert medium with a density gradient (such as sucrose or cesium chloride) and utilizing the difference in particle density with the medium density. This allows for automatic stratification of the gradient density liquid within the centrifuge tube under the action of a high-speed centrifuge. Traditional centrifuge tubes used for density gradient ultracentrifugation typically consist of a tube body and a cap, with the cap either flexibly connected to the tube body or screwed together. This structure is relatively simple, and samples inside the tube are prone to splashing out. Therefore, this study addresses these issues and led to this research. Utility Model Content

[0003] To address the shortcomings of existing technologies, this invention provides an automatic gradient density liquid stratification centrifuge tube for ultracentrifugation of exosomes, which solves the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model is implemented through the following technical solution: an automatic stratification centrifuge tube for ultracentrifuging of cell exosomes using gradient density liquid, comprising a tube body and a cap, wherein the upper end of the tube body is provided with an external thread, and the cap is screwed onto the external thread at the upper end of the tube body and seals against the upper end face of the tube body; the tube body is provided with an air inlet and a sampling port, the air inlet is provided with an air guiding and connecting component, and the sampling port is provided with a sealing sampling component;

[0005] The sealed sampling component includes a support, a cover plate, an L-shaped limiting block, a guide tube, and an elastic sealing element. The support is mounted on the pipe cap. The cover plate is coaxially arranged with the sampling port and one end is rotatably connected to the support. The L-shaped limiting block is symmetrically arranged with the support and is engaged with one end of the cover plate. The guide tube is mounted on the lower end of the sampling port, and the elastic sealing element is mounted on the lower end of the guide tube.

[0006] The aforementioned elastic sealing element includes a mounting ring and elastic diaphragms. The mounting ring is fixed on the outer wall of the lower end of the guide tube. The elastic diaphragms are all fan-shaped rubber plates arranged in a ring array on the inner wall of the mounting ring. The circular baffle formed by the elastic diaphragms seals the opening at the lower end of the guide tube.

[0007] The cover plate is symmetrically provided with wing plates at both ends. One wing plate is rotatably connected to the support, and the other wing plate is snapped and fixed with the L-shaped limiting block. A sealing gasket is provided on the lower end face of the cover plate.

[0008] The aforementioned air guiding and connecting component includes a valve seat, a valve sleeve, a sealing ring, and a valve stem. The valve seat is assembled at the air inlet position, the valve sleeve is embedded in the valve seat, an annular groove is formed on the inner circumferential surface of the valve seat, the sealing ring is disposed in the annular groove, and the valve stem is threadedly engaged with the valve sleeve.

[0009] The valve stem is provided with a sealing head at its lower end, and the sealing head is provided with a tapered boss at its lower end. A through hole is provided at the central axis position of the upper part of the valve stem, and ventilation channels are provided on both sides of the through hole.

[0010] A certain gap is left between the sealing head and the inner wall of the valve sleeve, and a nut is fixed to the upper end of the valve stem. Beneficial effects

[0011] This invention provides an automatic gradient density liquid stratification centrifuge tube for ultracentrifugation of exosomes. It offers the following advantages: This automatic gradient density liquid stratification centrifuge tube for ultracentrifugation of exosomes improves upon existing centrifuge tube caps by adding a gas-conducting communication component and a sealing sampling component. During ultracentrifugation, both the gas-conducting communication component and the sealing sampling component remain closed. The sample and gradient density liquid inside the centrifuge tube automatically stratify under centrifugal force. When extraction of the separated liquid is required, there is no need to open the cap; only the gas-conducting communication component and the sealing sampling component cover need to be opened. The sampling tube is then inserted into the guide tube and further penetrates through the elastic seal into the separated liquid for sampling. The structure is simple, the sealing performance is good, and the operation is convenient. There is no need to worry about splashing of the separated liquid during the entire sampling process, and all components can be reused after cleaning and disinfection. Attached Figure Description

[0012] Figure 1 This is a three-dimensional structural diagram of the gradient density liquid automatic stratification centrifuge tube for ultracentrifugation of cell exosomes according to the present invention.

[0013] Figure 2 This is an isometric structural diagram of the cap described in this utility model.

[0014] Figure 3 This utility model Figure 2 A cross-sectional structural diagram.

[0015] Figure 4 This utility model Figure 3 A magnified schematic diagram of the structure at position a.

[0016] In the diagram: 1. Pipe body; 2. Pipe cap; 3. Support; 4. Cover plate; 5. L-shaped limiting block; 6. Guide tube; 7. Mounting ring; 8. Elastic diaphragm; 9. Wing plate; 10. Sealing gasket; 11. Valve seat; 12. Valve sleeve; 13. Sealing ring; 14. Valve stem; 1401. Sealing head; 1402. Conical boss; 1403. Through hole; 1404. Vent channel; 1405. Nut. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Example: Refer to the appendix of the instruction manual Figure 1-4As can be seen, this application specifically designs an automatic stratification centrifuge tube for ultracentrifugation of cell exosomes using gradient density liquid, including a tube body 1 and a cap 2. The upper end of the tube body 1 is provided with an external thread, and the cap 2 is screwed onto the external thread at the upper end of the tube body 1 and seals with the upper end face of the tube body 1. The tube body 1 is provided with an air inlet and a sampling port. The air inlet is provided with a gas guiding and connecting component, and the sampling port is provided with a sealing sampling component. The sealing sampling component includes a support 3, a cover plate 4, an L-shaped limiting block 5, a guide tube 6, and an elastic sealing element. The support 3 is located on the cap. 2. The cover plate 4 is coaxially arranged with the sampling port and one end is rotatably connected to the support 3. The L-shaped limiting block 5 is symmetrically arranged with the support 3 and is engaged with one end of the cover plate 4. The guide tube 6 is set on the lower end of the sampling port. The elastic sealing element is set on the lower end of the guide tube 6. The elastic sealing element includes a mounting ring 7 and elastic diaphragm 8. The mounting ring 7 is fixed on the outer wall of the lower end of the guide tube 6. The elastic diaphragm 8 are all fan-shaped rubber plates and are arranged in a ring array on the inner wall of the mounting ring 7. The circular baffle formed by the elastic diaphragm 8 seals the opening at the lower end of the guide tube 6. The cover plate 4 has symmetrically arranged wing plates 9 at both ends. One wing plate 9 is rotatably connected to the support 3, and the other wing plate 9 is snapped and fixed to the L-shaped limiting block 5. A sealing gasket 10 is provided on the lower end face of the cover plate 4. The existing centrifuge tube cap 2 is improved by adding a gas guiding communication component and a sealing sampling component to the cap 2. During the ultracentrifugation operation, the gas guiding communication component and the sealing sampling component are both in a closed state. The sample and gradient density liquid in the centrifuge tube automatically separate into layers under the action of centrifugal force. When it is necessary to extract the separated liquid, there is no need to open it. The cap 2 only requires opening the gas-conducting connection component and pressing one end of the cover plate 4 to compress the sealing gasket 10, pushing the wing plate 9 at one end of the cover plate 4 out from the L-shaped limiting block 5, thereby opening the sealing sampling component cover plate 4. The sampling tube is then inserted into the guide tube 6 and further passes through the elastic diaphragm flap 8 inside the mounting ring 7 to reach the separation liquid for sampling. The structure is simple, with a double sealing design, good sealing performance, and convenient operation. During the entire sampling process, there is no need to worry about the separation liquid splashing, and all components can be reused after cleaning and disinfection.

[0019] In a preferred embodiment, the aforementioned air-guiding and connecting component includes a valve seat 11, a valve sleeve 12, a sealing ring 13, and a valve stem 14. The valve seat 11 is assembled at the air inlet position, the valve sleeve 12 is embedded in the valve seat 11, an annular groove is formed on the inner circumferential surface of the valve seat 11, the sealing ring 13 is disposed in the annular groove, and the valve stem 14 is threadedly engaged with the valve sleeve 12. A sealing head 1401 is provided at the lower end of the valve stem 14, and a conical boss 1402 is provided at the lower end of the sealing head 1401. A through hole 1403 is formed at the central axis position of the upper part of the valve stem 14, and air passages 1404 are formed on both sides of the through hole 1403. A certain gap is left between the sealing head 1401 and the inner wall of the valve sleeve 12. A nut 1405 is fixed at the upper end of the valve stem 14. During centrifugal operation, the valve stem 14 is in the position of... Figure 4In the state shown, the side wall of the sealing head 1401 presses against the sealing ring 13 to achieve a sealing effect. When sampling is required, the nut 1405 at the upper end of the valve stem 14 is rotated to drive the valve stem 14 to rotate and the valve stem 14 moves upward under the action of the thread. The upward movement of the valve stem 14 drives the sealing head 1401 and the conical head to move upward synchronously. When the conical head contacts the sealing ring 13, the seal gradually fails, and the pipe body 1 can be connected to the external environment through the gap between the valve sleeve 12 and the sealing head 1401, the vent 1404 and the through hole 1403, thereby effectively improving the convenience of sampling operations.

[0020] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, the phrase "comprising an element defined as..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0021] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automatic stratification centrifuge tube for ultracentrifuging exosomes using gradient density liquid, comprising a tube body and a cap, characterized in that, The upper end of the tube body is provided with an external thread, and the tube cap is screwed onto the external thread at the upper end of the tube body and is sealed to the upper end face of the tube body. The tube body is provided with an air inlet and a sampling port. The air inlet is provided with an air guiding and connecting component, and the sampling port is provided with a sealing sampling component. The sealed sampling component includes a support, a cover plate, an L-shaped limiting block, a guide tube, and an elastic sealing element. The support is mounted on the pipe cap. The cover plate is coaxially arranged with the sampling port and one end is rotatably connected to the support. The L-shaped limiting block is symmetrically arranged with the support and is engaged with one end of the cover plate. The guide tube is mounted on the lower end of the sampling port, and the elastic sealing element is mounted on the lower end of the guide tube.

2. The automatic stratification centrifuge tube for ultracentrifugation of exosomes according to claim 1, characterized in that, The elastic sealing element includes a mounting ring and elastic diaphragms. The mounting ring is fixed on the outer wall of the lower end of the guide tube. The elastic diaphragms are all fan-shaped rubber plates arranged in a ring array on the inner wall of the mounting ring. The circular baffle formed by the elastic diaphragms seals the opening at the lower end of the guide tube.

3. The automatic stratification centrifuge tube for ultracentrifugation of exosomes according to claim 1, characterized in that, The cover plate is symmetrically provided with wing plates at both ends. One wing plate is rotatably connected to the support, and the other wing plate is snapped and fixed with the L-shaped limiting block. A sealing gasket is provided on the lower end face of the cover plate.

4. The automatic stratification centrifuge tube for ultracentrifugation of exosomes according to claim 1, characterized in that, The air guiding and connecting component includes a valve seat, a valve sleeve, a sealing ring, and a valve stem. The valve seat is assembled at the air inlet position, the valve sleeve is embedded in the valve seat, an annular groove is formed on the inner circumferential surface of the valve seat, the sealing ring is disposed in the annular groove, and the valve stem is threadedly engaged with the valve sleeve.

5. The automatic stratification centrifuge tube for ultracentrifugation of exosomes according to claim 4, characterized in that, The valve stem is provided with a sealing head at its lower end, and the sealing head is provided with a conical boss at its lower end. A through hole is provided at the central axis position of the upper part of the valve stem, and ventilation channels are provided on both sides of the through hole.

6. The automatic stratification centrifuge tube for ultracentrifugation of exosomes according to claim 5, characterized in that, A certain gap is left between the sealing head and the inner wall of the valve sleeve, and a nut is fixed to the upper end of the valve stem.

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