Polymerase chain reaction (PCR) and capillary electrophoresis method IISI detection kit

Abstract

The utility model relates to the technical field of kits, and discloses a PCR and capillary electrophoresis method IISI detection kit, which comprises a kit body and a kit cover, the top of the kit body is rotatably connected with the kit cover through a hinge, an inner container is arranged inside the kit body, the inner wall of the inner container is provided with a placing groove, the inner walls of the kit body and the kit cover are both provided with a protective assembly, and the protective assembly is connected with the kit cover through a hinge. The limiting assembly is arranged on the inner wall of the containing groove, the limiting assembly comprises a support sliding on the inner wall of the containing groove, and the inner wall of the support is rotationally connected with a guide wheel through a connecting shaft; the support drives the guide wheel to retract into the inner container by moving the shifting block, the first spring is compressed at the moment, then the RCR pipe is inserted into the containing groove and located in the arc-shaped groove, then the shifting block is loosened, the support drives the guide wheel to automatically reset through resilience force of the first spring to clamp and fix the RCR pipe, and the device is suitable for the RCR pipes of different sizes. The practicability of the kit is improved.

Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, specifically to a PCR and capillary electrophoresis IISI detection kit. Background Technology

[0002] PCR (polymerase chain reaction) and capillary electrophoresis are two commonly used techniques in molecular biology. They are often used together to detect and analyze DNA. PCR technology can amplify specific DNA fragments, while capillary electrophoresis is used to separate and analyze these amplified DNA fragments. Test kits combining these two technologies have a variety of clinical applications, depending on the design of the kit and the gene or mutation being detected.

[0003] According to the utility model patent with announcement number CN221317272U, a multiplex PCR detection kit, which relates to the field of PCR detection technology, its beneficial effect is as follows: when removing the PCR tube, the threaded cap is rotated, and the threaded cap drives the threaded rod to rotate inside the nut, causing it to rise. After rising, the bearing at the bottom of the threaded rod and the placement rack will slowly rise from the inside of the placement slot, thereby exposing the PCR tube bottle body placed on the placement rack. After the PCR tube bottle body is exposed, it is convenient to pick it up.

[0004] In the above technology, the RCR tube is placed in a placement slot. Since the size of the placement slot is fixed, only RCR tubes that are compatible with it can be placed. When placing RCR tubes of different sizes, the tubes may become unstable or fail to fit. This technology is not suitable for RCR tubes of different sizes and has limited applicability. Utility Model Content

[0005] The purpose of this invention is to provide a PCR and capillary electrophoresis IISI detection kit to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a PCR and capillary electrophoresis IISI detection kit, comprising a box body and a box lid, wherein the top of the box body is rotatably connected to the box lid via a hinge, the inside of the box body is provided with an inner liner, the inner wall of the inner liner is provided with a placement groove, and the inner walls of both the box body and the box lid are provided with protective components.

[0007] A limiting component is disposed on the inner wall of the placement groove. The limiting component includes a bracket that slides on the inner wall of the placement groove. A guide wheel is rotatably connected to the inner wall of the bracket via a connecting shaft. A first spring is fixedly connected to one side of the bracket, and one end of the first spring is fixedly connected to the inner wall of the inner liner.

[0008] Preferably, the limiting component further includes an arc-shaped groove formed in the inner wall of the inner liner, and a lever is fixedly connected to the top of the bracket, the top of the lever penetrating the top of the inner liner.

[0009] Preferably, the limiting component further includes a limiting block fixed to one side of the bracket, and the inner wall of the inner liner is provided with a limiting groove that cooperates with the limiting block.

[0010] Preferably, the protective component includes a fixing cylinder that is fixed to the inner wall of the box body and the inner wall of the box cover respectively, and the inner wall of the fixing cylinder has a buffer chamber.

[0011] Preferably, the protective assembly further includes a connecting cylinder that slides on the inner wall of the buffer chamber, and a connecting plate is fixedly connected to the top of the connecting cylinder.

[0012] Preferably, the protective assembly further includes a second spring disposed on the outside of the connecting cylinder, one end of the second spring being fixedly connected to the top of the fixed cylinder, and the other end of the second spring being fixedly connected to the bottom of the connecting plate.

[0013] Preferably, rubber blocks are provided at the four corners of the bottom of the box and the four corners of the top of the lid.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] This invention uses a movable lever to retract the support and guide wheel into the inner chamber, at which point the first spring is compressed. Then, the RCR tube is inserted into the placement slot and positioned in the arc-shaped groove. After releasing the lever, the rebound force of the first spring causes the support and guide wheel to automatically reset and clamp the RCR tube in place. By adjusting the distance between multiple sets of supports, it can be adapted to RCR tubes of different sizes, thus improving the practicality of the reagent kit. Attached Figure Description

[0016] Figure 1 A schematic diagram of a preferred embodiment of the PCR and capillary electrophoresis IISI detection kit provided by this utility model;

[0017] Figure 2 A schematic diagram of the inner liner and placement groove provided by this utility model;

[0018] Figure 3 This is a partial cross-sectional structural schematic diagram provided for this utility model;

[0019] Figure 4 for Figure 2 Enlarged schematic diagram of the structure at point A in the middle;

[0020] Figure 5 for Figure 3 Enlarged schematic diagram of the structure at point B.

[0021] In the diagram: 1. Box body; 2. Box lid; 3. Inner liner; 4. Placement slot; 5. Limiting component; 51. Bracket; 52. Guide wheel; 53. First spring; 54. Arc-shaped groove; 55. Pulley; 56. Limiting block; 57. Limiting groove; 6. Protective component; 61. Fixing cylinder; 62. Buffer chamber; 63. Connecting cylinder; 64. Connecting plate; 65. Second spring; 7. Rubber block. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figures 1-5 As shown, the PCR and capillary electrophoresis IISI detection kit includes a box body 1 and a box cover 2. The top of the box body 1 is connected to the box cover 2 by a hinge. The box body 1 is provided with an inner liner 3. The inner wall of the inner liner 3 is provided with a placement groove 4. The inner walls of both the box body 1 and the box cover 2 are provided with protective components 6.

[0024] The limiting component 5 is disposed on the inner wall of the placement groove 4. The limiting component 5 includes a bracket 51 that slides on the inner wall of the placement groove 4. A guide wheel 52 is rotatably connected to the inner wall of the bracket 51 via a connecting shaft. A first spring 53 is fixedly connected to one side of the bracket 51. One end of the first spring 53 is fixedly connected to the inner wall of the inner liner 3. The limiting component 5 also includes an arc-shaped groove 54 formed in the inner wall of the inner liner 3. A lever 55 is fixedly connected to the top of the bracket 51. The top of the lever 55 penetrates the top of the inner liner 3. The limiting component 5 also includes a limiting block 56 fixed to one side of the bracket 51. A limiting groove 57 that cooperates with the limiting block 56 is formed in the inner wall of the inner liner 3.

[0025] The movable lever 55 retracts the bracket 51 into the inner liner 3, and the guide wheel 52 moves synchronously with the bracket 51. Through the cooperation of the limiting block 56 and the limiting groove 57, the bracket 51 can maintain stable movement. At this time, the first spring 53 is in a compressed state. Then, the RCR tube is inserted into the placement groove 4 and its bottom is located in the arc-shaped groove 54. Then, the lever 55 is released, and the rebound force of the first spring 53 causes the bracket 51 to drive the guide wheel 52 to automatically reset and clamp and fix the RCR tube. By adjusting the distance of the bracket 51, it is easy to insert RCR tubes of different sizes into the placement groove 4, which improves the practicality of the reagent kit.

[0026] The protective component 6 includes a fixing cylinder 61 that is fixed to the inner wall of the box body 1 and the box cover 2 respectively. The inner wall of the fixing cylinder 61 is provided with a buffer chamber 62. The protective component 6 inside the box cover 2 is the same as the protective component 6 inside the box body 1. There are eight fixing cylinders 61, and four are grouped together. A group of fixing cylinders 61 corresponds to the four corners of the connecting plate 64.

[0027] The protective component 6 also includes a connecting cylinder 63 that slides on the inner wall of the buffer chamber 62. A connecting plate 64 is fixedly connected to the top of the connecting cylinder 63, and the inner liner 3 is located on the connecting plate 64.

[0028] The protective component 6 also includes a second spring 65 disposed on the outside of the connecting cylinder 63. One end of the second spring 65 is fixedly connected to the top of the fixed cylinder 61, and the other end of the second spring 65 is fixedly connected to the bottom of the connecting plate 64. By ensuring that the initial force of the multiple sets of second springs 65 is greater than the weight of the inner liner 3, the second springs 65 are prevented from compressing automatically.

[0029] After the RCR tube is installed, rotate the box cover 2 and move one of the protective components 6 so that the connecting plate 64 in the mechanism is located on top of the RCR tube. During the transportation of the reagent kit, the two sets of protective components 6 arranged symmetrically can buffer the RCR tube inside. When the inner liner 3 is vibrated, it will move up and down inside the box 1. At this time, the connecting plate 64 can drive the connecting cylinder 63 to move into the buffer chamber 62 in the fixed cylinder 61, and cause multiple sets of second springs 65 to compress and rebound and absorb and release energy, thereby achieving the buffering and protection effect of the RCR tube and avoiding damage to the RCR tube inside the inner liner 3.

[0030] Rubber blocks 7 are provided at the four corners of the bottom of the box body 1 and the four corners of the top of the box cover 2; the rubber blocks 7 can protect the four corners of the reagent kit and prevent damage from bumps.

[0031] Working principle: Moving the lever 55 causes the support 51 to retract the guide wheel 52 into the inner liner 3. At this time, the first spring 53 is compressed. Then, the RCR tube is inserted into the placement slot 4 and located in the arc-shaped groove 54. Then, the lever 55 is released. The rebound force of the first spring 53 causes the support 51 to automatically reset the guide wheel 52 and clamp and fix the RCR tube. By adjusting the distance of the support 51, it is easy to insert RCR tubes of different sizes into the placement slot 4, which improves the practicality of the reagent kit.

[0032] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0033] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A PCR and capillary electrophoresis IISI detection kit, comprising a housing (1), characterized in that, Also includes: The lid (2) is connected to the top of the box body (1) by a hinge. The box body (1) is provided with an inner liner (3). The inner wall of the inner liner (3) is provided with a placement groove (4). The inner walls of the box body (1) and the lid (2) are both provided with protective components (6). A limiting component (5) is disposed on the inner wall of the placement groove (4). The limiting component (5) includes a bracket (51) that slides on the inner wall of the placement groove (4). A guide wheel (52) is rotatably connected to the inner wall of the bracket (51) via a connecting shaft. A first spring (53) is fixedly connected to one side of the bracket (51). One end of the first spring (53) is fixedly connected to the inner wall of the inner liner (3).

2. The PCR and capillary electrophoresis IISI detection kit according to claim 1, characterized in that: The limiting component (5) also includes an arc-shaped groove (54) formed on the inner wall of the inner liner (3), and a lever (55) is fixedly connected to the top of the bracket (51), the top of the lever (55) penetrating the top of the inner liner (3).

3. The PCR and capillary electrophoresis IISI detection kit according to claim 2, characterized in that: The limiting component (5) also includes a limiting block (56) fixed to one side of the bracket (51), and the inner wall of the inner liner (3) is provided with a limiting groove (57) that cooperates with the limiting block (56).

4. The PCR and capillary electrophoresis IISI detection kit according to claim 1, characterized in that: The protective component (6) includes a fixing cylinder (61) fixed to the inner wall of the box body (1) and the box cover (2) respectively, and the inner wall of the fixing cylinder (61) is provided with a buffer chamber (62).

5. The PCR and capillary electrophoresis IISI detection kit according to claim 4, characterized in that: The protective component (6) further includes a connecting cylinder (63) that slides on the inner wall of the buffer chamber (62), and a connecting plate (64) is fixedly connected to the top of the connecting cylinder (63).

6. The PCR and capillary electrophoresis IISI detection kit according to claim 5, characterized in that: The protective component (6) further includes a second spring (65) disposed on the outside of the connecting cylinder (63), one end of the second spring (65) being fixedly connected to the top of the fixed cylinder (61), and the other end of the second spring (65) being fixedly connected to the bottom of the connecting plate (64).

7. The PCR and capillary electrophoresis IISI detection kit according to claim 4, characterized in that: Rubber blocks (7) are provided at the four corners of the bottom of the box body (1) and the four corners of the top of the box lid (2).

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