Equipment for colorimetric detection of glucose content

Abstract

The utility model relates to the field of glucose detection, and particularly discloses a device for colorimetric detection of glucose content, which comprises a reaction unit used for incubating and reacting a detection sample and a reaction solution; the detection unit is used for performing colorimetric detection on the reacted solution; and the central control unit is used for controlling equipment operation and conveying a detection sample and a reaction solution. The reaction temperature is regulated and controlled by the central control unit, and the detection sample and the reaction solution are conveyed to realize automatic detection of incubation, reaction and color comparison of the detection sample and the reaction solution, so that the detection speed is greatly improved.

Description

Technical Field

[0001] This utility model relates to the field of glucose detection, and more specifically, to a colorimetric device for detecting glucose content. Background Technology

[0002] Glucose plays a vital role in living organisms and serves as a clinical indicator for certain diseases. The level of glucose in the blood is a crucial indicator of overall health; many diseases, such as diabetes and hypoglycemia, are caused by glucose imbalances. Insufficient or excessive glucose in the body can lead to hypoglycemia or diabetes, respectively. Diabetes, as a disorder of glucose metabolism, significantly impacts health; therefore, quantitative detection of blood glucose levels is essential for its diagnosis and treatment. Due to the widespread presence of glucose in organisms, the food industry, and environmental pollution, many techniques, including colorimetric, fluorescence, electrochemical, chemiluminescence, and photoelectrochemical methods, have been developed for glucose detection. However, existing methods are complex, involve numerous steps, and require frequent transfer of reaction solutions, resulting in slow detection speeds and poor accuracy.

[0003] Therefore, there is an urgent need for a device that can detect glucose content with high precision and high efficiency. Summary of the Invention

[0004] In view of this, the present invention proposes a colorimetric detection device for glucose content, aiming to solve the problems of existing detection methods being complex to operate, having many steps, requiring frequent transfer of reaction solutions, resulting in slow detection speed and poor accuracy.

[0005] This utility model provides a colorimetric device for detecting glucose content, characterized in that it includes:

[0006] The reaction unit is used to mix, incubate, and react the reaction solution and the test sample.

[0007] The detection unit is used to perform colorimetric detection on the solution after the reaction.

[0008] The central control unit, connected to the reaction unit and the detection unit, is used to hold the shell for transporting the test sample and reaction solution, and to analyze the colorimetric detection results of the detection unit.

[0009] Furthermore, the reaction unit includes:

[0010] The reaction chamber is a square box with a trapezoidal slider on the top surface of its side wall. The reaction chamber is used to provide a reaction site for the test sample and the reaction solution.

[0011] A sliding cover is located above the reaction chamber, and a trapezoidal groove is provided at the bottom of the sliding cover, which is engaged with the slider;

[0012] Eight temperature sensors are installed and located at the bends inside the reaction chamber to detect the temperature information inside the reaction chamber.

[0013] A sealing gasket is fitted and connected to the inner wall of the reaction chamber and penetrates the inner wall of the reaction chamber;

[0014] The air inlet is a circular through hole, and multiple inlets are provided on the inner side wall of the reaction chamber.

[0015] The exhaust vent is a circular through-hole, symmetrically arranged with the air inlet;

[0016] A shaking table is connected to the bottom surface of the reaction chamber, and the top surface of the shaking table is provided with multiple limiting grooves.

[0017] The bottom of the reaction vessel is fitted and connected to the shaking table via the limiting groove;

[0018] The injection tube extends into the interior of the reaction vessel through the top surface on one side, and is connected to the sealing gasket on the other side.

[0019] The suction tube extends from the top of the reaction vessel to the bottom of the reaction vessel on one side, and is connected to the sealing gasket on the other side.

[0020] Furthermore, the detection unit includes:

[0021] The testing chamber is a square box used to provide a testing area;

[0022] A flip-top is located on top of the reaction chamber;

[0023] A light source, connected to the inner wall of the detection chamber, is used to provide a detection light source;

[0024] Receiver 250 is connected to the other inner wall of the detection chamber and is symmetrically arranged with the light source;

[0025] The base has its top surface connected to the bottom surface of the light source and the receiver 250, and its bottom surface connected to the bottom surface of the detection chamber.

[0026] A light shield, connected to the top surface of the light source and receiver 250, is used to prevent external light sources from affecting the detection.

[0027] Furthermore, the base has multiple mounting slots evenly distributed in the center of its top surface, and these mounting slots are arranged parallel to the light source.

[0028] Furthermore, the reaction unit also includes:

[0029] The cuvette has its bottom fitted into the mounting groove;

[0030] The cuvette lid is fitted and connected to the light-shielding plate at the top, and the bottom of the cuvette lid fits into the top of the cuvette.

[0031] The liquid inlet pipe is a cylindrical tube, with one end connected to the side wall of the reaction chamber and the other end connected to the cuvette cap.

[0032] Furthermore, the central control unit includes:

[0033] The central control compartment is a square box.

[0034] A central control system is located at the top of the central control compartment and is connected to the inner wall of the central control compartment.

[0035] The top surface of the heat circulation system is connected to the bottom surface of the central control system, and the bottom surface of the heat circulation system is connected to the bottom surface of the hollow chamber.

[0036] The power supply is connected to the bottom surface of the central control system on its top surface and to the bottom surface of the hollow compartment. The power supply is used to provide power.

[0037] Furthermore, the reaction vessel includes:

[0038] The test tube cap has an annular sealing groove embedded in its bottom surface.

[0039] The test tube is a cylindrical tube with an annular sealing ring at the top, which fits into the sealing groove.

[0040] Furthermore, the top surface of the central control system is equipped with multiple solution tanks, cleaning fluid tanks, and a control panel. The solution tanks are used to hold test samples and reaction solutions, the cleaning fluid tanks are used to hold cleaning fluid, and the control panel is used to display test results and control the operation of the equipment.

[0041] Furthermore, the central control system is provided with a first pipe, a second pipe, and a third pipe on its side;

[0042] One end of the first pipe passes through the side wall of the reaction chamber and is connected to the injection pipe, and the other end is connected to the solution chamber. The first pipe is used to transport the test sample and reaction solution in the solution chamber to the reaction container through the injection pipe.

[0043] The second pipe passes through the side wall of the reaction chamber and is connected to the liquid suction pipe. The second pipe is used to draw the solution after the reaction in the reaction vessel.

[0044] The third pipe passes through the side wall of the detection chamber and is connected to the inlet pipe. The third pipe is used to inject the reacted solution into the cuvette.

[0045] Furthermore, an air inlet pipe and an air outlet pipe are provided on the two parallel outer side walls of the heat circulation system;

[0046] The air inlet pipe is connected to the air outlet, and the air outlet pipe is connected to the air inlet.

[0047] Compared with the prior art, the advantages of this utility model are as follows: by controlling the reaction temperature and transporting the test sample and reaction solution through the central control unit, the incubation, reaction and colorimetric detection of the test sample and reaction solution are realized, which greatly improves the detection efficiency and detection accuracy. Attached Figure Description

[0048] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0049] Figure 1 This is a three-dimensional structural view of a colorimetric glucose content detection device provided in one embodiment of the present invention.

[0050] Figure 2 This is a diagram showing the internal structure of the reaction unit in this invention.

[0051] Figure 3 This is a cross-sectional view of the reaction vessel in this utility model;

[0052] Figure 4 This is a cross-sectional view of the reaction chamber in this utility model;

[0053] Figure 5 This is a diagram showing the internal structure of the detection unit in this utility model;

[0054] Figure 6 This is a cross-sectional view of the detection unit in this utility model;

[0055] Figure 7 This is a diagram showing the internal structure of the central control unit in this utility model.

[0056] In the diagram: 100-Reaction unit; 110-Reaction chamber; 111-Slider; 120-Sliding cover; 121-Slide groove; 130-Temperature sensor; 140-Sealing gasket; 151-Air inlet; 152-Air outlet; 160-Reaction vessel; 161-Test tube; 162-Sealing ring; 163-Test tube cap; 164-Sealing groove; 170-Liquid suction tube; 180-Liquid injection tube; 190-Shaking table; 191-Limiting groove; 200-Detection unit; 210-Flip cover; 220-Light shield; 230-Liquid inlet tube; 240 - Light source; 250- Receiver; 260- Base; 261- Mounting slot; 270- Cuvette cover; 271- Through hole; 272- Limiting ring; 280- Cuvette; 290- Detection chamber; 300- Central control unit; 310- Central control chamber; 320- Central control system; 321- Solution chamber; 322- Cleaning solution chamber; 323- Control panel; 324- First pipe; 325- Second pipe; 326- Third pipe; 330- Thermal circulation system; 331- Air outlet duct; 332- Air inlet duct; 340- Power supply. Detailed Implementation

[0057] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0058] In the description of this application, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation; or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0059] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0060] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0061] See Figure 1-7 As shown, this embodiment provides a colorimetric device for detecting glucose content, characterized in that it includes:

[0062] The reaction unit 100 is used to mix, incubate, and react the reaction solution and the test sample.

[0063] The detection unit 200 is used to perform colorimetric detection on the solution after the reaction.

[0064] The central control unit 300 is connected to the reaction unit 100 and the detection unit 200. It is used to hold the shell for transporting the test sample and reaction solution, and to analyze the results of the colorimetric detection by the detection unit 200.

[0065] See Figure 1-7 As shown, in some embodiments of this utility model, the reaction unit 100 includes: a reaction chamber 110, which is a square box, with a trapezoidal slider 111 provided on the top surface of the side wall of the reaction chamber 110, and the reaction chamber 110 is used to provide a reaction site for the test sample and the reaction solution; a sliding cover 120, located above the reaction chamber 110, with a trapezoidal groove 121 provided at the bottom of the sliding cover 120, and the groove 121 fitting into the slider 111; eight temperature sensors 130, located at the bend inside the reaction chamber 110, used to detect the temperature information inside the reaction chamber 110; a sealing gasket 140, which fits into and penetrates the inner side wall of the reaction chamber 110; and an air inlet 151. Multiple circular through holes 271 are provided on the inner wall of the reaction chamber 110; an exhaust port 152, also a circular through hole 271, is symmetrically arranged with the air inlet 151; a shaking table 190 is connected to the bottom surface of the reaction chamber 110, and multiple limiting grooves 191 are provided on the top surface of the shaking table 190; a reaction container 160 is fitted and connected to the shaking table 190 at its bottom through the limiting grooves 191; a liquid injection pipe 180 extends into the interior of the reaction container 160 through the top surface of the reaction container 160 on one side, and is connected to the sealing gasket 140 on the other side; a liquid suction pipe 170 extends into the bottom of the reaction container 160 through the top surface of the reaction container on one side, and is connected to the sealing gasket 140 on the other side.

[0066] Specifically, the reaction chamber 110 is made of heat-insulating material; the slider 111 and the groove 121 are flexible colloids, and their interlocking can effectively prevent heat loss from the inside of the reaction chamber 110; the sealing gasket 140 is also a flexible colloid.

[0067] Understandably, the reaction chamber 110 made of heat-insulating material can prevent external temperature from affecting the internal temperature of the reaction chamber 110, while the slider 111, the groove 121 and the sealing gasket 140 greatly increase the airtightness of the reaction chamber 110.

[0068] Specifically, the shaking stage 190 has an eccentric structure inside, which allows the shaking stage 190 to perform slow eccentric movements when reacting with the solution and testing the sample, so that the reaction solution and the testing sample can be fully mixed.

[0069] See Figure 1-7 As shown, the detection unit 200 includes: a detection chamber 290, which is a square box for providing a detection area; a flip cover 210, which is located on top of the reaction chamber 110; a light source 240, which is connected to one inner wall of the detection chamber 290 for providing the detection light source 240; a receiver 250, which is connected to the other inner wall of the detection chamber 290 and is symmetrically arranged with the light source 240; a base 260, whose top surface is connected to the bottom surface of the light source 240 and the receiver 250, and whose bottom surface is connected to the bottom surface of the detection chamber 290; and a light shield 220, which is connected to the top surface of the light source 240 and the receiver 250 for preventing the external light source 240 from affecting the detection.

[0070] Specifically, the light source 240, receiver 250, and cuvette 280 constitute a spectrophotometer that converts the color change of the solution after the reaction into a light signal and transmits it to the central control unit 300.

[0071] See Figure 2 As shown, multiple mounting slots 261 are evenly arranged in the center of the top surface of the base 260, and the multiple mounting slots 261 are arranged parallel to the light source 240; the reaction unit 100 also includes: a cuvette 280, the bottom of which is fitted into the mounting slot 261; a cuvette cover 270, the top of which is fitted into the light shield 220, and the bottom of the cuvette cover 270 is fitted into the top of the cuvette 280; and a liquid inlet pipe 230, which is a cylindrical tube, one end of which is connected to the side wall of the reaction chamber 110, and the other end is connected to the cuvette cover 270.

[0072] Specifically, before starting the test, open the flip cover 210, place the cuvette 280 into the mounting slot 261, and insert the cuvette cover 270 into the cuvette 280.

[0073] See Figure 1-7 As shown, the central control unit 300 includes:

[0074] The central control compartment 310 is a square box; the central control system 320 is located on top of the central control compartment 310 and is connected to the inner wall of the central control compartment 310; the top surface of the heat circulation system 330 is connected to the bottom surface of the central control system 320, and the bottom surface of the heat circulation system 330 is connected to the bottom surface of the hollow compartment; the power supply 340 is connected to the bottom surface of the central control system 320 and the bottom surface of the hollow compartment, and the power supply 340 is used to provide power.

[0075] Specifically, the power supply 340 is used to provide power to the various components of the equipment to ensure that they can operate normally, and the central control unit 300 is used to control the thermal circulation system 330 to provide a suitable reaction temperature for the reaction unit 100 and to receive the light signal transmitted by the detection unit 200 and analyze and display the detection results.

[0076] See Figure 1-7 As shown, the reaction vessel 160 includes:

[0077] The test tube cap 163 has an annular sealing groove 164 embedded in its bottom surface; the test tube 161 is a cylindrical tube, and an annular sealing ring 162 is provided at the top of the test tube 161, which fits into the sealing groove 164.

[0078] Specifically, the sealing ring 162 is a flexible colloid and its size is slightly larger than that of the sealing groove 164, so that after the sealing ring 162 is embedded in the sealing groove 164, the sealing ring 162 can fully contact the sealing groove 164, which greatly improves the airtightness of the reaction vessel 160.

[0079] See Figure 1-7 As shown, the top surface of the central control system 320 is equipped with multiple solution tanks 321, cleaning solution tanks 322, and control panels 323. The solution tanks 321 are used to hold test samples and reaction solutions, the cleaning solution tanks 322 are used to hold cleaning solutions, and the control panels 323 are used to display test results and control the operation of the equipment.

[0080] See Figure 1-7 As shown, the central control system 320 has a first pipe 324, a second pipe 325, and a third pipe 326 on its side. One end of the first pipe 324 passes through the side wall of the reaction chamber 110 and is connected to the injection pipe 180, and the other end is connected to the solution chamber 321. The first pipe 324 is used to transport the test sample and reaction solution in the solution chamber 321 to the reaction container 160 through the injection pipe 180. The second pipe 325 passes through the side wall of the reaction chamber 110 and is connected to the suction pipe. The second pipe 325 is used to aspirate the solution after reaction in the reaction container 160. The third pipe 326 passes through the side wall of the detection chamber 290 and is connected to the inlet pipe 230. The third pipe 326 is used to inject the solution after reaction into the cuvette 280.

[0081] Specifically, the central control system is equipped with a peristaltic pump and a solenoid valve. The two work together and, through the first pipe 324, the second pipe 325 and the third pipe 326, can accurately draw in and discharge test samples and reaction solutions.

[0082] See Figure 1-7 As shown, the heat circulation system 330 has an air inlet pipe 332 and an air outlet pipe 331 on its two parallel outer side walls; the air inlet pipe 332 is connected to the air outlet 152, and the air outlet pipe 331 is connected to the air inlet 151.

[0083] Specifically, the air outlet duct 331 and the exhaust duct are connected to the exhaust port 152 and the air inlet 151 by being embedded inside the reaction chamber 110.

[0084] Specifically, the heat circulation system 330 is equipped with heating equipment and a fan. Air is drawn from inside the reaction chamber 110 through the air inlet pipe 332, heated by the heating equipment, and then transported back into the reaction chamber 110 through the exhaust pipe to form a circulating air duct.

[0085] See Figure 1-7 As shown, the specific method of using this utility model is as follows: Serum sample, glucose oxidase solution, buffer solution, TMB, and CuFe-TA nanozyme are placed into solution chamber 321 respectively. Then, cuvette 280 is placed into installation slot 261, and cuvette cap 270 is inserted into cuvette 280. Afterwards, the incubation temperature is set to 37℃ and the reaction temperature to 40℃ via control panel 323, and then the detection is started. During the detection process, the central control unit 300 first injects the serum sample and glucose oxidase solution into reaction container 160 and incubates at 37℃ for 30 minutes. Then, buffer solution, TMB, and CuFe-TA nanozyme are added and mixed and reacted at 40℃ for 15 minutes. The reacted solution is then aspirated and injected into cuvette 280 for colorimetric detection. After the detection is completed, the results are displayed on control panel 323, thus completing the detection.

[0086] After the test is completed, the tester clicks "clean" on the control panel 323. Then, the central control system 320 injects the cleaning solution into the reaction vessel 160. The shaking table 190 increases its power and shakes for 10 seconds to ensure that the cleaning solution is thoroughly cleaned before being sucked out. This process is repeated three times. After completion, the heat circulation system 330 increases its heating power to dry the reaction vessel 160, thus completing the cleaning process.

[0087] It will be understood by those skilled in the art that the above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A colorimetric device for detecting glucose content, characterized in that, include: The central control unit is a square box used to hold the test sample and reaction solution transported by the shell, as well as to analyze the colorimetric detection results of the detection unit; The reaction unit, connected to the outer wall of the central control unit, is used to mix, incubate, and react the reaction solution and test sample delivered by the central control unit. The detection unit has one outer wall connected to the outer wall of the central control unit and another outer wall connected to the reaction unit, and is used to perform colorimetric detection on the reaction solution delivered by the central control unit.

2. The colorimetric device for detecting glucose content according to claim 1, characterized in that, The reaction unit includes: The reaction chamber is a square box, and a trapezoidal slider is provided on the top surface of the side wall of the reaction chamber. A sliding cover is located above the reaction chamber, and a trapezoidal groove is provided at the bottom of the sliding cover, which is engaged with the slider; Eight temperature sensors are installed and located at the bends inside the reaction chamber to detect the temperature information inside the reaction chamber. A sealing gasket is fitted and connected to the inner wall of the reaction chamber and penetrates the inner wall of the reaction chamber; The air inlet is a circular through hole, and multiple inlets are provided on the inner side wall of the reaction chamber. The exhaust vent is a circular through-hole, symmetrically arranged with the air inlet; A shaking table is connected to the bottom surface of the reaction chamber, and the top surface of the shaking table is provided with multiple limiting grooves. The bottom of the reaction vessel is fitted and connected to the shaking table via the limiting groove; The injection tube extends into the interior of the reaction vessel through the top surface on one side, and is connected to the sealing gasket on the other side. The suction tube extends from the top of the reaction vessel to the bottom of the reaction vessel on one side, and is connected to the sealing gasket on the other side.

3. The colorimetric device for detecting glucose content according to claim 2, characterized in that, The detection unit includes: The testing chamber is a square box used to provide a testing area; A flip-top is located on the top of the reaction chamber and is hinged to the side wall of the detection chamber; A light source, connected to the inner wall of the detection chamber, is used to provide a detection light source; The receiver is connected to the other inner wall of the detection chamber and is symmetrically arranged with the light source; The base has its top surface connected to the bottom surface of the light source and the receiver, and its bottom surface connected to the bottom surface of the detection chamber. A light-shielding plate, connected to the top surface of the light source and receiver, is used to prevent external light sources from affecting the detection.

4. The colorimetric device for detecting glucose content according to claim 3, characterized in that, The base has multiple mounting slots evenly distributed in the center of its top surface, and these mounting slots are arranged parallel to the light source.

5. The colorimetric device for detecting glucose content according to claim 4, characterized in that, The reaction unit further includes: The cuvette has its bottom fitted into the mounting groove; The cuvette lid has its top fitted into the light-shielding plate, and the bottom of the cuvette lid is fitted into the top of the cuvette. The liquid inlet pipe is a cylindrical tube, with one end connected to the side wall of the reaction chamber and the other end connected to the cuvette cap.

6. The colorimetric device for detecting glucose content according to claim 5, characterized in that, The central control unit includes: The central control compartment is a square box. A central control system is located at the top of the central control compartment and is connected to the inner wall of the central control compartment. The top surface of the heat circulation system is connected to the bottom surface of the central control system, and the bottom surface of the heat circulation system is connected to the bottom surface of the central control compartment. The power supply is connected to the bottom surface of the central control system on its top surface and to the bottom surface of the central control compartment. The power supply is used to provide power.

7. The colorimetric device for detecting glucose content according to claim 6, characterized in that, The reaction vessel includes: The test tube cap has an annular sealing groove embedded in its bottom surface; The test tube is a cylindrical tube with an annular sealing ring at the top, which fits into the sealing groove.

8. The colorimetric device for detecting glucose content according to claim 7, characterized in that, The top surface of the central control system is equipped with multiple solution tanks, cleaning fluid tanks, and a control panel. The solution tanks are used to hold test samples and reaction solutions, the cleaning fluid tanks are used to hold cleaning fluid, and the control panel is used to display test results and control the operation of the equipment.

9. The colorimetric device for detecting glucose content according to claim 8, characterized in that, The central control system is provided with a first pipe, a second pipe, and a third pipe on its side; One end of the first pipe passes through the side wall of the reaction chamber and is connected to the injection pipe, and the other end is connected to the solution chamber. The first pipe is used to transport the test sample and reaction solution in the solution chamber to the reaction container through the injection pipe. The second pipe passes through the side wall of the reaction chamber and is connected to the liquid suction pipe. The second pipe is used to draw the solution after the reaction in the reaction vessel. The third pipe passes through the side wall of the detection chamber and is connected to the inlet pipe. The third pipe is used to inject the reacted solution into the cuvette.

10. The colorimetric device for detecting glucose content according to claim 9, characterized in that, The heat circulation system has an air inlet pipe and an air outlet pipe installed on its two parallel outer side walls. The air inlet pipe is connected to the air outlet, and the air outlet pipe is connected to the air inlet.

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