Sterile automatic liquid adding device

Abstract

The utility model belongs to the technical field of liquid adding devices, and particularly relates to a sterile automatic liquid adding device which comprises a liquid storage assembly, a liquid adding assembly and a conveying assembly. The liquid storage assembly comprises a fixing support, an inverted liquid storage bottle is arranged on the fixing support, the liquid storage bottle is used for storing sterile liquid, a first connector and a second connector which are communicated with the interior of the liquid storage bottle are formed in the liquid storage bottle, and the second connector is connected with a sterile air filter; the liquid adding assembly comprises a sealing bottle, a sealing cover is connected to the sealing bottle, a dropper is arranged on the sealing cover, and the upper end and the lower end of the dropper are located outside and inside the sealing bottle respectively; the conveying assembly comprises a wireless remote controller and a peristaltic pump, the wireless remote controller is used for starting or closing the peristaltic pump and adjusting the rotating speed of the peristaltic pump, the two ends of a hose of the peristaltic pump are connected with water pipes, and the two water pipes are connected with the first connector of the liquid storage bottle and the upper end of the dropper respectively; the problems that according to a traditional liquid adding method, the liquid adding amount is difficult to control, operation is tedious, efficiency is low, and sterile liquid is prone to being polluted are solved.

Description

Technical Field

[0001] This utility model belongs to the technical field of liquid dispensing devices, specifically relating to a sterile automated liquid dispensing device. Background Technology

[0002] In the microspore culture technology of rapeseed, the microspore extraction, microspore chromosome doubling, and microspore suspension culture stages require frequent addition of different sterile liquids for treatment. For example, after disinfection of rapeseed buds, they need to be washed three times with pre-cooled sterile water; during bud grinding, a sterile 13% sucrose solution is needed; after collecting microspores by centrifuge tubes and filtering them through a filter membrane, they need to be washed three times with sterile NLN13 medium solution; during microspore chromosome doubling culture, a sterile NLN16 medium solution containing colchicine is needed for liquid culture; and during microspore suspension culture, a sterile NLN13 medium solution is needed again.

[0003] The commonly used method for adding liquid is as follows: put sterile liquid into a glass bottle, open the glass bottle cap in the clean bench, lift the glass bottle by hand, align the glass bottle opening directly above the opening of the culture dish to be added, and immediately seal the glass bottle opening with the glass bottle cap after adding the liquid to prevent contamination.

[0004] The above-mentioned liquid addition method has the following disadvantages: 1. The glass bottles containing sterile liquid are heavy and difficult to lift manually. In addition, the petri dishes usually have a small diameter, making it difficult to control the amount of liquid added during the process, resulting in either too much or too little liquid being added. 2. Since liquid addition is frequent throughout the microspore culture process, manual liquid addition is extremely inconvenient. Furthermore, the repeated opening and closing of the glass bottle caps increases the risk of contamination of the sterile liquid. 3. Staff usually need to process multiple rapeseed microspore materials, and these materials cannot be mixed. This results in each material needing to repeat the above-mentioned liquid addition process, leading to low work efficiency. Utility Model Content

[0005] The purpose of this invention is to provide a sterile automated liquid dispensing device to solve the problems of difficult control of liquid volume, cumbersome operation, low efficiency, and easy contamination of sterile liquids in traditional liquid dispensing methods.

[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0007] An automated aseptic liquid dispensing device includes a liquid storage component, a liquid dispensing component, and a delivery component. The liquid storage component includes a fixed support with an inverted liquid storage bottle on it. The liquid storage bottle is used to store sterile liquid. The liquid storage bottle has a first interface and a second interface communicating with the interior of the liquid storage bottle. A sterile air filter is connected to the second interface. The liquid dispensing component includes a sealed bottle with a threaded sealing cap. A dropper is provided on the sealing cap, with its upper and lower ends located outside and inside the sealed bottle, respectively. The delivery component includes a wireless remote control and a peristaltic pump. The wireless remote control is used to start or stop the peristaltic pump and to adjust its rotation speed. Both ends of the peristaltic pump hose are connected to water pipes, which are connected to the first interface of the liquid storage bottle and the upper end of the dropper, respectively.

[0008] Furthermore, the liquid dispensing assembly also includes a telescopic bracket with a fixing plate on it. The fixing plate is connected to the upper end of the telescopic bracket via a universal ball joint. The fixing plate is equipped with a first magnet. The sealing cap is equipped with a second magnet, which can be attracted to the first magnet to fix the dropper on the telescopic bracket.

[0009] Furthermore, the liquid storage assembly also includes a refrigerator, and the fixing bracket, liquid storage bottle and sterile air filter are all placed inside the refrigerator.

[0010] Furthermore, the sterile air filter is used to prevent the liquid in the storage bottle from being contaminated. When the peristaltic pump draws the liquid, the outside air enters the storage bottle after being filtered by the sterile air filter, so that the pressure inside the storage bottle is always equal to the outside atmospheric pressure. The sterile air filter is placed higher than the storage bottle.

[0011] Furthermore, the sealing cap has a central hole, the dropper is installed in the central hole, and a sealing ring is provided between the central hole and the dropper.

[0012] Compared with the prior art, the present invention has the following beneficial technical effects:

[0013] 1. This utility model, by setting up a liquid storage component, a liquid addition component, and a delivery component, allows for liquid addition by removing the sealing cap from the sealed bottle, aligning the dropper nozzle with the culture dish to be added, and then starting the peristaltic pump. The sterile liquid in the storage bottle enters the culture dish through the lower end of the dropper under the action of the peristaltic pump, thus completing the liquid addition operation. By setting up a peristaltic pump and a wireless remote control, the amount of liquid added at one time can be precisely controlled. Compared with the traditional manual liquid addition method, the liquid addition operation has a higher degree of automation, is more convenient and faster, and not only effectively improves the liquid addition efficiency, but also avoids the situation of adding too much or too little liquid.

[0014] 2. By setting up a sealed bottle and a dropper, the small diameter of the dropper effectively reduces the risk of contamination of sterile liquids compared to the traditional method of directly pouring liquids from a glass bottle.

[0015] 3. During microspore culture, the sterile liquid needs to be kept at a low temperature when adding liquid. Traditional manual liquid addition methods take a long time, and the temperature of the sterile liquid will rise after the glass bottle is placed in the clean bench for too long, which will affect the microspore culture. However, in this invention, the storage bottle is always kept in the refrigerator during the liquid addition process, which can ensure that the sterile liquid is always kept at a low temperature and meets the temperature requirements. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the liquid storage component of this utility model;

[0018] Figure 3 This is an exploded view of the liquid addition assembly of this utility model;

[0019] Figure 4 This is a schematic diagram of the conveying component of this utility model.

[0020] In the diagram: Liquid storage assembly 1, fixed bracket 11, liquid storage bottle 12, bottle cap 13, first interface 14, second interface 15, sterile air filter 16, refrigerator 17, liquid filling assembly 2, sealing bottle 21, sealing cap 22, second magnet 221, dropper 23, sealing ring 24, base 25, telescopic rod 26, fixing plate 27, first magnet 271, universal ball joint 28, conveying assembly 3, peristaltic pump 31, hose 32, clean bench 4, and storage table 5. Detailed Implementation

[0021] The following detailed description illustrates the specific implementation method:

[0022] Example

[0023] like Figure 1 As shown, a sterile automated liquid dispensing device includes a liquid storage component 1, a liquid dispensing component 2, and a delivery component 3.

[0024] like Figure 2As shown, the liquid storage assembly 1 includes a refrigerator 17, a fixed bracket 11 placed inside the refrigerator 17, and a sterile air filter 16. An inverted liquid storage bottle 12 is mounted on the fixed bracket 11. The liquid storage bottle 12 is used to store sterile liquid. A cap 13 is threaded onto the liquid storage bottle 12. The cap 13 has a first interface 14 and a second interface 15 communicating with the interior of the liquid storage bottle 12. The second interface 15 is connected to the sterile air filter 16. The sterile air filter 16 is used to prevent contamination of the liquid inside the liquid storage bottle 12. The sterile air filter 16 is positioned higher than the liquid storage bottle 12 to prevent the liquid in the liquid storage bottle 12 from flowing back into the sterile air filter 16. During the liquid addition process, the refrigerator 17 creates a low-temperature environment for the liquid storage bottle 12, ensuring that the sterile liquid remains at a low temperature, meeting the temperature requirements for microspore culture.

[0025] like Figure 3 As shown, the liquid addition assembly 2 includes a sealed bottle 21, a sealed cap 22 threaded onto the sealed bottle 21, a second magnet 221 bonded to the sealed cap 22, a central hole on the sealed cap 22, and a dropper 23 installed in the central hole. The upper and lower ends of the dropper 23 are located outside and inside the sealed bottle 21, respectively, and a sealing ring 24 is fixed between the dropper 23 and the central hole. The liquid addition assembly 2 also includes a telescopic bracket, which includes a base 25 and a telescopic rod 26 fixed on the base 25. The upper end of the telescopic rod 26 is connected to a fixing plate 27 through a universal ball joint 28, and a first magnet 271 is bonded to the fixing plate 27.

[0026] When adding liquid, the liquid adding assembly 2 should be placed on the clean bench 4. Then, the sealing cap 22 should be removed from the sealed bottle 21, and the second magnet 221 should be attached to the first magnet 271 to fix the dropper 23 on the fixing plate 27. The height and tilt angle of the dropper 23 should be adjusted by the telescopic rod 26 and the universal ball head 28 so that the lower end of the dropper 23 is aligned with the culture dish to be added. After the liquid adding operation is completed, the dropper 23 should be immediately resealed in the sealed bottle 21 through the sealing cap 22 to reduce the risk of contamination. Since the diameter of the dropper 23 is small, the risk of sterile liquid being contaminated can be further reduced. Moreover, there is no need to hold the glass bottle during the liquid adding process, which saves time and effort.

[0027] like Figure 4 As shown, the delivery assembly 3 includes a wireless remote control and a peristaltic pump 31. The wireless remote control is used to start or stop the peristaltic pump 31 and to adjust the speed of the peristaltic pump 31. Both ends of the peristaltic pump 31 hose 32 are connected to water pipes (not shown in the figure). The two water pipes are respectively connected to the first interface 14 of the storage bottle 12 and the upper end of the dropper 23. When the peristaltic pump 31 draws liquid, the outside air enters the storage bottle 12 after being filtered by the sterile air filter 16, so that the pressure inside the storage bottle 12 is always equal to the outside atmospheric pressure.

[0028] During the liquid addition process, the sterile liquid in the storage bottle 12 enters the dropper 23 through the water pipe under the action of the peristaltic pump 31, and finally enters the culture dish to be added through the lower end of the dropper 23. By setting the peristaltic pump 31 and the wireless remote control together, the liquid addition time and the amount of liquid added at one time can be automatically controlled. The liquid addition operation is convenient and quick, which not only effectively improves the liquid addition efficiency, but also avoids the situation of adding too much or too little liquid.

[0029] The specific implementation is as follows:

[0030] 1. Connect the liquid storage bottle 12, the liquid addition component 2, and the delivery component 3 of the liquid storage component 1 in the ultra-clean workbench 4 to ensure that the entire pipeline and the liquid storage bottle 12 are free from contamination;

[0031] 2. Then, place the liquid storage bottle 12 upside down on the fixed support 11, and place the fixed support 11, the liquid storage bottle 12 and the sterile air filter 16 in the refrigerator 17 of the shelf 5, and place the peristaltic pump 31 on the shelf 5.

[0032] 3. Sterilize in the ultra-clean workbench for 30 minutes;

[0033] 4. Remove the sealing cap 22 from the sealing bottle 21 and immediately seal the sealing bottle 21 with the ordinary bottle cap 13 to prevent contamination of the sealing bottle 21. Then, attach the sealing cap 22 to the fixing plate 27, place the culture dish to be added at the lower end of the dropper 23, and adjust the height and angle of the dropper 23 so that the lower end of the dropper 23 is directly facing the mouth of the culture dish.

[0034] 5. The peristaltic pump 31 is controlled by a wireless remote control to deliver the sterile liquid in the storage bottle 12 to the dropper 23 through the water pipe, and finally drip it into the petri dish.

[0035] 6. After adding liquid to one culture dish, turn off the peristaltic pump 31 and move the next culture dish to the lower end of the dropper 23. Repeat step 5 to add liquid again.

[0036] 7. After all culture dishes have been filled with liquid, turn off the peristaltic pump 31 and reseal the dropper 23 in the sealed bottle 21 through the sealing cap 22.

[0037] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A sterile automated liquid addition device, characterized by: The system includes a liquid storage assembly (1), a liquid dispensing assembly (2), and a delivery assembly (3). The liquid storage assembly (1) includes a fixed bracket (11) on which an inverted liquid storage bottle (12) is mounted. The liquid storage bottle (12) is used to store sterile liquid. The liquid storage bottle (12) has a first interface (14) and a second interface (15) communicating with the interior of the liquid storage bottle (12). A sterile air filter (16) is connected to the second interface (15). The liquid dispensing assembly (2) includes a sealed bottle (21) with threads on it. A sealing cap (22) is connected, and a dropper (23) is provided on the sealing cap (22). The upper and lower ends of the dropper (23) are located outside and inside the sealing bottle (21), respectively. The delivery assembly (3) includes a wireless remote control and a peristaltic pump (31). The wireless remote control is used to start or stop the peristaltic pump (31) and to adjust the speed of the peristaltic pump (31). Both ends of the hose (32) of the peristaltic pump (31) are connected to water pipes. The two water pipes are connected to the first interface (14) of the storage bottle (12) and the upper end of the dropper (23), respectively.

2. The sterile automated liquid addition device of claim 1, wherein: The liquid dispensing assembly (2) also includes a telescopic bracket, on which a fixing plate (27) is provided. The fixing plate (27) is connected to the upper end of the telescopic bracket via a universal ball joint (28). A first magnet (271) is provided on the fixing plate (27); a second magnet (221) is provided on the sealing cap (22). The second magnet (221) can be attracted to the first magnet (271) to fix the dropper (23) on the telescopic bracket.

3. The sterile automated liquid addition device of any of claims 1-2, wherein: The liquid storage assembly (1) also includes a refrigerator (17), and the fixed bracket (11), liquid storage bottle (12), and sterile air filter (16) are all placed inside the refrigerator (17).

4. The aseptic automated liquid addition device of claim 3, wherein: The sterile air filter (16) is used to prevent the liquid in the storage bottle (12) from being contaminated. When the peristaltic pump (31) draws the liquid, the outside air enters the storage bottle (12) after being filtered by the sterile air filter (16), so that the pressure inside the storage bottle (12) is always equal to the outside atmospheric pressure. The sterile air filter (16) is placed higher than the storage bottle (12).

5. The aseptic automated liquid addition device of claim 4, wherein: The sealing cap (22) has a central hole, the dropper (23) is installed in the central hole, and a sealing ring (24) is provided between the central hole and the dropper (23).

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