Sample detection pore plate suitable for Raman spectrometer

Abstract

The utility model belongs to the field of sample detection, and particularly relates to a sample detection pore plate suitable for a Raman spectrometer. Comprising a substrate, a plurality of detection holes for sample detection are formed in the upper surface of the substrate, and a plurality of silica gel pads are arranged at the bottoms of the detection holes; the chip is placed on the silica gel pad, and a vertical height difference H is kept between the surface of the chip and the detection hole; during detection, a certain distance exists between the bottom of a light source of the Raman spectrometer and the surface of the pore plate. The detection chip is placed in each hole site, samples are physically separated, and cross contamination of the samples caused by liquid flow among the samples is avoided in a sample adding process and an endowing process.

Description

Technical Field

[0001] This utility model belongs to the field of sample testing, specifically a sample detection well plate suitable for Raman spectrometers. Background Technology

[0002] In existing detection technologies, most methods employ eight detection substrates fixed on the same glass slide for sample analysis (e.g., Figure 4 (As shown). However, this design has significant drawbacks: due to insufficient physical isolation between samples, cross-contamination is highly likely to occur during operation, leading to reduced accuracy of test results. This risk is further amplified in high-throughput or high-sensitivity detection scenarios, affecting the reproducibility of experiments and the reliability of clinical validation. Therefore, a sample detection structure that can avoid cross-contamination is needed. Utility Model Content

[0003] To achieve the above objectives, this invention proposes a sample detection well plate suitable for Raman spectrometers. Several silicone pads are provided at the bottom of the detection wells, and a vertical height difference H is maintained between the chip surface and the detection wells, forming physical barriers between the detection chips to avoid cross-contamination.

[0004] To achieve the above objectives, the present invention specifically employs the following technical means:

[0005] A sample detection well plate suitable for Raman spectrometers includes a substrate with a plurality of detection wells for sample detection on its upper surface, and a plurality of silicone pads at the bottom of the detection wells; the chip is placed on the silicone pads, and a vertical height difference H is maintained between the chip surface and the detection wells; during detection, there is a certain distance between the bottom of the light source of the Raman spectrometer and the surface of the well plate.

[0006] Preferably, the sample includes three silicone pads, each with a thickness of 1 mm. The distance from the chip surface to the detection well is 4 mm. During detection, the distance from the bottom of the Raman spectrometer's light source to the surface of the well plate is 7 mm. The sample detection well plate is a 96-well plate.

[0007] Compared with the prior art, the present invention has the following beneficial technical effects:

[0008] By using the detection substrate in conjunction with a 96-well plate and placing three 1mm thick silicone pads at the bottom of the detection wells, a stable physical isolation barrier is formed between the chip and the detection wells. This design completely solves the problem of cross-contamination between samples caused by insufficient physical isolation when the detection substrate is fixed to the same glass slide in traditional methods, significantly improving the accuracy of the test results and the reliability of clinical validation.

[0009] By controlling the thickness of the silicone pads and the vertical height difference between the chip surface and the detection wells, a precise distance is maintained between the bottom of the Raman spectrometer's light source and the well plate surface. The Raman focusing step eliminates the need for repeated manual adjustments, improving the limit of detection and the stability of linear experimental results. The 96-well plate structure allows for a 12-fold increase in sample volume compared to traditional 8-well plates, and it is compatible with standard automated equipment, making it particularly suitable for large-scale clinical screening scenarios. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of an improved detection hole in a specific embodiment of this utility model;

[0011] Figure 2 This is a structural schematic diagram of a specific embodiment of the present invention;

[0012] Figure 3 This is a side view of a specific embodiment of the present invention;

[0013] Figure 4 This is a schematic diagram of the background technology;

[0014] Figure 5 These are the detection results of different samples in the background technology.

[0015] The numbers in the diagram represent: 1. Detection hole; 2. Silicone pad; 3. Chip; 4. Raman spectrometer; 5. Glass slide. Detailed Implementation

[0016] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of the present invention. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0017] The accompanying drawings are for illustrative purposes only and are schematic diagrams, not actual pictures, and should not be construed as limiting the invention. To better illustrate the embodiments of the invention, some parts in the drawings may be omitted, enlarged, or reduced, and do not represent the actual product dimensions. It is understandable to those skilled in the art that some well-known structures and their descriptions are omitted in the drawings.

[0018] Please see Figure 4When eight detection substrates are fixed on the same glass slide in a traditional method, insufficient physical isolation can lead to cross-contamination of sample solutions due to tilting or spillage. Sample 1 served as a negative control, sample 2 was negative, and samples 3 and 4 were positive. When multiple detection substrate chips are placed on the slide, cross-contamination of sample 2 occurred due to accidental tilting or liquid spillage during sample addition, incubation, or washing processes, resulting in a weakly positive test result.

[0019] Please see Figures 1-3 The detection hole 1 of this invention has three silicone pads 2 at its bottom, each 1mm thick. The silicone pads 2 support the chip 3, creating a 4mm vertical height difference between the surface of the chip 3 and the detection hole 1. This design effectively avoids cross-contamination between samples through a physical isolation barrier.

[0020] The sample detection plate adopts a 96-well plate structure, which can accommodate 96 independent detection units at a time. The chip 3 is fixed to the bottom of the detection well 1 by the silicone pad 2, ensuring that there is no risk of liquid leakage between the detection units. The structure shown in the figure is compatible with automated equipment, significantly improving the detection throughput.

[0021] The bottom of the light source in the Raman spectrometer 4 maintains a precise distance of 7 mm from the surface of the well plate. This distance is achieved through the thickness of the silicone pad 2 and the height difference of the chip 3, ensuring that Raman focusing requires no manual adjustment. In actual operation, the Raman spectrometer 4 can be fixed with an iron frame to further ensure detection stability.

[0022] The specific implementation steps are as follows: Use a clamp to fix the handheld Raman spectrometer to a fixture with an iron frame, thus fixing the height of the Raman spectrometer from the detection stage. Embed the Raman detection substrate chip in a 96-well plate. Insert three silicone pads (1mm thick each, totaling 3mm) into the bottom of the plate. The distance from the chip surface to the well is 4mm. This ensures that Raman spectral signals from different sample wells are acquired at the same height when using the Raman spectrometer for spectral detection and collecting specific Raman peak signals. During detection, the distance from the bottom of the portable Raman spectrometer's light source to the surface of the 96-well plate is 7mm. The first well in the first row of the 96-well plate contains the substrate solution to remove background noise interference. 4μL of substrate solution + 4μL of DNA are dropped onto the chip in the well of the 96-well plate. Incubate at 65℃ for 10 minutes, rinse with pure water, and observe the results using the Raman spectrometer.

[0023] When using glass slides as the substrate for the chip, the lack of physical separation between samples during the detection process, such as sample addition and incubation, easily leads to cross-contamination. By using a 96-well plate as the substrate chip carrier, the detection chip is placed in each well of the 96-well plate, with physical separation between samples. This eliminates cross-contamination caused by liquid flow during sample addition and distribution. Furthermore, the 96-well plate can be covered during incubation, preventing aerosol contamination from water baths or incubators.

Claims

1. A sample detection well plate suitable for a Raman spectrometer, comprising a substrate, the upper surface of which is provided with a plurality of detection wells (1) for sample detection, characterized in that, The bottom of the detection hole is provided with several silicone pads (2); a chip (3) is placed on the silicone pad (2), and the chip (3) surface and the detection hole maintain a vertical height difference H; during detection, there is a certain distance between the bottom of the light source of the Raman spectrometer (4) and the surface of the aperture plate.

2. The sample detection well plate suitable for a Raman spectrometer of claim 1, wherein, Includes 3 silicone pads (2), each silicone pad is 1mm thick.

3. The sample detection well plate suitable for a Raman spectrometer of claim 1, wherein, The distance from the surface of the chip (3) to the detection hole (1) is 4 mm.

4. The sample detection well plate suitable for a Raman spectrometer of claim 1, wherein, During the detection, the distance from the bottom of the light source of the Raman spectrometer (4) to the surface of the aperture plate is 7 mm.

5. The sample detection well plate suitable for a Raman spectrometer of claim 1, wherein, The sample detection plate is a 96-well plate.

Images