Exosome extracting and filtering device

Abstract

The utility model relates to the technical field of exosomes, and discloses an exosome extracting and filtering device which comprises a vertical supporting pipe used for providing longitudinal supporting force, a second reinforcing plate installed on the inner side of the vertical supporting pipe and used for providing an installation position, and a third filtering tank installed on the front portion of the second reinforcing plate and used for providing an installation position. The second filtering tank is installed on the upper portion of the first filtering tank and used for providing a filtering effect on exosomes, the storage device is installed on the upper portion of the third filtering tank, the soft pipe is installed on the outer side of the soft pipe and used for limiting movement, and the switch valve is used for controlling extraction of the exosomes. The sequence of the filtering part can be changed as required, the filtering requirements under different conditions are met, and the device is simple in structure and convenient to popularize and apply.

Description

Technical Field

[0001] This utility model relates to the field of exosome technology, and in particular to an exosome extraction and filtration device. Background Technology

[0002] Exosomes are nanoscale extracellular vesicles secreted by most cells. They contain components such as mRNA, miRNA, proteins, and lipids. In 2013, James Rothman, Randy Schekman, and Thomas Südhof were awarded the Nobel Prize in Physiology or Medicine for their discovery of vesicle transport mechanisms. As an important class of extracellular vesicles, exosomes have attracted widespread attention due to their functions in immune regulation, antigen presentation, participation in intercellular signal transduction, and applications in disease diagnosis and treatment. Before in-depth research on exosomes, the extraction and filtration of exosomes is fundamental to all subsequent experiments; only highly active and stable exosomes can ensure the quality and reliability of subsequent experiments.

[0003] Currently, there are some exosome extraction and filtration devices, but they all have some problems in their application. The main problems are that the filtration sequence cannot be changed, the device structure is complex, and it is difficult to promote and apply them. Summary of the Invention

[0004] In order to overcome the shortcomings of the existing technology, the purpose of this utility model is to provide an exosome extraction and filtration device, whose filtration section can be changed in order as needed to meet the filtration needs of different situations. The device has a simple structure and is easy to promote and apply.

[0005] This utility model is achieved using the following technical solution: an exosome extraction and filtration device, comprising: a vertical support tube, which provides longitudinal support; a second reinforcing plate, which is installed inside the vertical support tube to provide an installation position; a third filter tank, which is installed in front of the second reinforcing plate to provide filtration of exosomes; a storage container, which is installed on the upper part of the third filter tank; a flexible tube, on the outside of which a side plate is installed to restrict movement; and a switch valve for controlling the extraction of exosomes.

[0006] Preferably, the vertical support tubes are symmetrically installed on both sides of the second reinforcing plate, the second reinforcing plate is installed below the first reinforcing plate, the first reinforcing plate is installed inside the vertical support tube, a transverse support tube for providing lateral support force is installed at the bottom of the vertical support tube, and a support angle is installed at the bottom of the transverse support tube.

[0007] Preferably, a second filter canister is installed on the left side of the third filter canister, a first filter canister is installed on the left side of the second filter canister, an injection pipe is installed on the left side of the first filter canister, and an injection port for inputting the exosomes to be filtered is installed on the left side of the injection pipe.

[0008] Preferably, a first output pipe is installed on the right side of the third filter tank for outputting the filtered exosomes, and a power pump is installed on the right side of the first output pipe for providing power.

[0009] Preferably, a second output pipe is connected to the right side of the storage device. The outer side of the second output pipe is fixed to the lower part of the storage device by a fixing clamp. An adapter pipe is installed on the upper part of the second output pipe. One end of the adapter pipe is connected to the storage device, and the other end of the adapter pipe is connected to the power pump.

[0010] Preferably, a spring is installed at the rear of the side plate, a vertical input tube is installed at the bottom of the soft tube, a horizontal output tube is installed at the bottom of the vertical input tube, a buffer tube is installed on the right side of the horizontal output tube to prevent excessive force during exosome extraction, a second output tube is installed at the bottom right side of the buffer tube, and a shell is installed on the outside of the buffer tube.

[0011] Preferably, the top of the switching valve is equipped with an external connecting pipe for outputting the filtered and extracted exosomes, the bottom of the switching valve is equipped with a second adapter, the bottom of the second adapter is equipped with a handle, the bottom of the handle is equipped with a first adapter, and the bottom of the first adapter is equipped with an extraction tube.

[0012] Preferably, the bottom of the extraction tube is connected to the top of the flexible tube.

[0013] Preferably, the first filter canister 203 is equipped with a first filter material 208, the second filter canister 204 is equipped with a second filter material 209, and the third filter canister 205 is equipped with a third filter material 210.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] 1. The present invention relates to an exosome extraction and filtration device, which, through the installation of a first filter tank, a second filter tank, and a third filter tank, allows the placement of the required filter material. The material can be replaced and the replacement sequence is flexible, making it widely applicable and achieving the effect of filtering exosomes.

[0016] 2. The present invention provides an exosome extraction and filtration device, which can store filtered exosomes through an installed storage tank to prepare for the subsequent extraction stage, and can output the exosomes in the storage tank through an output pipe for the next extraction step.

[0017] 3. The exosome extraction and filtration device of this utility model can buffer the impact force when exosomes are output through the installed buffer tube to prevent the device from vibrating. The side plate and spring installed on the outside of the soft tube can increase the pressure and rebound force on the outside to keep the exosome output of the soft tube stable. Furthermore, a power device can be added to further increase the shrinkage and enlargement of the soft tube and increase the output of exosomes. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the oblique structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the front view structure of this utility model;

[0020] Figure 3 This is a schematic diagram of the left-side structure of this utility model;

[0021] Figure 4 This is a partial structural schematic diagram of this utility model.

[0022] In the diagram: 101, Horizontal support pipe; 102, Support angle; 103, Vertical support pipe; 104, First reinforcing plate; 105, Second reinforcing plate; 201, Injection port; 202, Injection pipe; 203, First filter tank; 204, Second filter tank; 205, Third filter tank; 206, First output pipe; 207, Power pump; 208, First filter material; 209, Second filter material; 210, Third filter material; 301, Adapter pipe; 302, Storage tank; 303, Second output pipe; 304, Fixing clamp; 401, Outer shell; 402, Buffer pipe; 403, Horizontal output pipe; 404, Vertical input pipe; 405, Flexible pipe; 406, Side plate; 407, Spring; 501, Extraction pipe; 502, First adapter; 503, Handle; 504, Second adapter; 505, Switch valve; 506, External pipe. Detailed Implementation

[0023] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0024] The purpose of this invention is to address the shortcomings of existing technologies by providing an exosome extraction and filtration device.

[0025] Example 1

[0026] This embodiment provides an exosome extraction and filtration device, referring to... Figures 1 to 3 As shown, it includes: a vertical support tube 103, which provides longitudinal support; a second reinforcing plate 105, which is installed inside the vertical support tube 103 to provide an installation position; a third filter tank 205, which is installed in front of the second reinforcing plate 105 to provide filtration of exosomes; a storage tank 302, which is installed on the upper part of the third filter tank 205; a flexible tube 405, on the outside of which a side plate 406 is installed to restrict movement; and a switch valve 505 to control the extraction of exosomes.

[0027] Vertical support tubes 103 are symmetrically installed on both sides of the second reinforcing plate 105. The second reinforcing plate 105 is installed below the first reinforcing plate 104. The first reinforcing plate 104 is installed inside the vertical support tube 103. A transverse support tube 101 for providing lateral support force is installed at the bottom of the vertical support tube 103. A support angle 102 is installed at the bottom of the transverse support tube 101. Through the transverse support tube 101 for providing lateral support force and the vertical support tube 103 for providing longitudinal support force, stable support can be provided for the entire device, preventing the device from tipping over or causing other dangers during use.

[0028] The third filter tank 205 has a second filter tank 204 installed on its left side, and the second filter tank 204 has a first filter tank 203 installed on its left side. The first filter tank 203 has an injection pipe 202 installed on its left side, and the injection pipe 202 has an injection port 201 for inputting the exosomes to be filtered installed on its left side. The first filter tank 203 has a first filter material 208 installed inside, the second filter tank 204 has a second filter material 209 installed inside, and the third filter tank 205 has a third filter material 210 installed inside. The first filter tank 203 and the second filter tank 204, the second filter tank 204 and the third filter tank 205 are connected by a threaded connection, which is convenient for disassembly and ensures sealing. The required filter materials, such as polymer membrane materials and lipophilic gels, can be put into them. The materials are replaceable, so as to achieve the effect of filtering exosomes.

[0029] The third filter tank 205 is equipped with a first output pipe 206 on the right side, which is used to output the filtered exosomes. The first output pipe 206 is equipped with a power pump 207 on the right side, which is used to provide power. The power pump 207 used in this utility model is a commonly used pump on the market, such as the model LS-0416, which is powered by DC12V voltage and has a pumping speed of 5.0L / min.

[0030] A second output pipe 303 is connected and installed on the right side of the storage device 302. The outer side of the second output pipe 303 is fixed to the lower part of the storage device 302 by a fixing clamp 304. An adapter pipe 301 is installed on the upper part of the second output pipe 303. One end of the adapter pipe 301 is connected to the storage device 302, and the other end of the adapter pipe 301 is connected to the power pump 207. The installed storage device 302 can store the filtered exosomes, which can be prepared for the subsequent extraction stage. The exosomes in the storage device 302 can be output through the second output pipe 303 for the next extraction action.

[0031] Example 2

[0032] This embodiment provides an exosome extraction and filtration device, referring to... Figures 2 to 3 As shown, a spring 407 is installed at the rear of the side plate 406, a vertical input tube 404 is installed at the bottom of the flexible tube 405, a horizontal output tube 403 is installed at the bottom of the vertical input tube 404, a buffer tube 402 is installed on the right side of the horizontal output tube 403 to prevent excessive impact during exosome extraction, a second output tube 303 is installed at the bottom right side of the buffer tube 402, and a shell 401 is installed on the outside of the buffer tube 402. The installed buffer tube 402 can buffer the impact during exosome output and prevent vibration of the device. The side plate 406 and spring 407 installed on the outside of the flexible tube 405 can increase the pressure and rebound force on the outside, keep the exosome output of the flexible tube 405 stable, and can also add a power device to further increase the shrinkage and enlargement of the flexible tube 405 and increase the exosome output.

[0033] The top of the switch valve 505 is equipped with an external pipe 506 for outputting the filtered and extracted exosomes. The bottom of the switch valve 505 is equipped with a second adapter 504, the bottom of the second adapter 504 is equipped with a handle 503, the bottom of the handle 503 is equipped with a first adapter 502, the bottom of the first adapter 502 is equipped with an extraction tube 501, and the bottom of the extraction tube 501 is connected to the top of the flexible tube 405.

[0034] Working principle: Exosomes flow into the first filter tank 203 through the injection port 201 and injection pipe 202. The first filter tank 203 contains the first filter material 208, the second filter tank 204 contains the second filter material 209, and the third filter tank 205 contains the third filter material 210. After triple filtration by the first filter tank 203, the second filter tank 204, and the third filter tank 205, the filtered exosomes are pumped by the power pump 207 through the first output pipe 206 and the transfer pipe 301 to the storage tank 302. The exosomes in the storage tank 302 can be transported to the buffer pipe 402 through the second output pipe 303. The fixing clamp 304 provides a fixing function for the second output pipe 303, and the buffer pipe 402 can buffer the transported exosomes to prevent excessive impact from causing device vibration. To prevent adverse effects, the exosomes in the buffer tube 402 are transported to the vertical input tube 404 via the horizontal output tube 403, and then to the flexible tube 405. The side plate 406 and spring 407 installed on the outside of the flexible tube 405 can increase the pressure and rebound force on the outside, keeping the output of exosomes from the flexible tube 405 stable. In addition, a power device can be added to further increase the shrinkage and enlargement of the flexible tube 405, increasing the output of exosomes. The exosomes in the flexible tube 405 are controlled by the handle 503 connected to the first adapter 502 and the switch valve 505 on the upper part of the handle 503 via the extraction tube 501. Finally, the exosomes are output by the outer tube 506. The combined installation of the horizontal support tube 101, support angle 102, vertical support tube 103, first reinforcing plate 104 and second reinforcing plate 105 provides stable support for the entire device.

[0035] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An exosome extraction and filtration device, characterized in that, include: Vertical support tube (103), the vertical support tube (103) is used to provide longitudinal support force; The second reinforcing plate (105) is installed inside the vertical support tube (103) to provide an installation position; The third filter canister (205) is installed in front of the second reinforcing plate (105) and is used to provide filtration of exosomes; Storage container (302), said storage container (302) is installed on the upper part of the third filter tank (205); A flexible tube (405), with a side plate (406) mounted on the outside of the flexible tube (405) for restricting movement; and a switch valve (505) for controlling the extraction of exosomes.

2. The exosome extraction filtration device of claim 1, wherein: The vertical support tube (103) is symmetrically installed on both sides of the second reinforcing plate (105). The second reinforcing plate (105) is installed at the lower part of the first reinforcing plate (104). The first reinforcing plate (104) is installed inside the vertical support tube (103). A transverse support tube (101) for providing transverse support force is installed at the bottom of the vertical support tube (103). A support angle (102) is installed at the bottom of the transverse support tube (101).

3. The exosome extraction filtration device of claim 1, wherein: The third filter tank (205) is equipped with a second filter tank (204) on its left side, and the second filter tank (204) is equipped with a first filter tank (203) on its left side. The first filter tank (203) is equipped with an injection tube (202) on its left side, and the injection tube (202) is equipped with an injection port (201) for inputting the exosomes to be filtered on its left side.

4. The exosome extraction filtration device of claim 1, wherein: The third filter tank (205) is equipped with a first output pipe (206) on the right side for outputting the filtered exosomes. A power pump (207) is also installed on the right side of the first output pipe (206) for providing power.

5. The exosome extraction filtration device of claim 1, wherein: The storage device (302) is connected to a second output pipe (303) on the right side. The outer side of the second output pipe (303) is fixed to the lower part of the storage device (302) by a fixing clamp (304). An adapter pipe (301) is installed on the upper part of the second output pipe (303). One end of the adapter pipe (301) is connected to the storage device (302), and the other end of the adapter pipe (301) is connected to the power pump (207).

6. The exosome extraction filtration device of claim 1, wherein: A spring (407) is installed at the rear of the side plate (406), a vertical input tube (404) is installed at the bottom of the soft tube (405), a horizontal output tube (403) is installed at the bottom of the vertical input tube (404), a buffer tube (402) is installed on the right side of the horizontal output tube (403) to prevent excessive force during exosome extraction, a second output tube (303) is installed at the bottom right side of the buffer tube (402), and a shell (401) is installed on the outside of the buffer tube (402).

7. The exosome extraction filtration device of claim 1, wherein: The switch valve (505) is provided with an external pipe (506) at the top, which is used to output the filtered and extracted exosomes.

8. The exosome extraction filtration device of claim 7, wherein: The bottom of the extraction pipe (501) is connected with the top of the soft tube (405).

9. An exosome extraction and filtration device according to claim 3, characterized in that: The first filter tank (203) is internally provided with a first filter material (208), the second filter tank (204) is internally provided with a second filter material (209), and the third filter tank (205) is internally provided with a third filter material (210).

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