Flow cytometer separation liquid drop anti-adhesion flow guide device

Abstract

The utility model discloses a flow cytometer sorting liquid drop anti-adhesion flow guide device which comprises an installation shell, an installation cover is fixed on the side wall of the installation shell, a detection mechanism is arranged on the outer side of the installation cover, an installation support is fixed on the inner wall of the installation shell through bolts, a material storage mechanism is arranged on the top of the installation support, and a flow cytometer is arranged on the material storage mechanism. A sorting mechanism is arranged at the bottom of the storage mechanism, a flow guide mechanism is arranged at the bottom of the sorting mechanism, and an airflow compensation mechanism is arranged on the outer side of a flow guide pipe. The flow guide mechanism is arranged to guide sheath fluid, so that sheath fluid residues can be effectively reduced, cross contamination is avoided, frequent shutdown for cleaning is not needed, the continuity of the flow cytometer during use is guaranteed, flow guide parameters of the flow guide mechanism are adjusted through the airflow compensation mechanism, and the flow cytometer adapts to viscosity and liquid drop speed changes of different samples; and the angle deviation of liquid drop collision is reduced, so that the adhesion risk of liquid drops with different specifications is reduced.

Description

Technical Field

[0001] This utility model relates to the field of flow cytometer technology, and in particular to a flow cytometer droplet anti-adhesion and flow guiding device. Background Technology

[0002] Sorting flow cytometers separate cells by encapsulating them in a sheath fluid to form a stream, and then electrifying the cells within the stream and passing them through an electric field. The flow cytometer with sorting capabilities is equipped with an ultrasonic oscillating crystal plate on the nozzle of the flow chamber. This oscillation device breaks the liquid stream ejected from the nozzle into millions of tiny droplets, dispersing the flowing cells within these droplets. An electrical pulse signal is then applied to the stream, causing all the droplets to become charged. Cells with different charges flow through a constant electrostatic field of several thousand volts composed of two voltage deflection plates. The charged droplets are deflected according to the properties of their charge and finally fall into their respective collection tubes. Uncharged droplets enter the waste container, thus achieving cell sorting.

[0003] Compared with existing technologies, the following problems exist: Traditional flow guiding devices cause cell droplet residue due to electrostatic adsorption and surface tension imbalance, leading to cross-contamination and requiring frequent shutdowns for cleaning. Secondly, fixed flow guiding parameters cannot adapt to changes in sample viscosity and droplet velocity, resulting in droplet impact angle deviation and increasing the risk of adhesion. To address these issues, we propose a flow cytometer droplet sorting anti-adhesion flow guiding device. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a flow cytometer droplet sorting anti-adhesion guiding device.

[0005] The present invention solves its technical problem through the following technical solution: it includes a mounting housing, a mounting cover is fixed to the side wall of the mounting housing, a detection mechanism is provided on the outside of the mounting cover, a mounting bracket is fixed to the inner wall of the mounting housing by bolts, a material storage mechanism is provided on the top of the mounting bracket, a sorting mechanism is provided at the bottom of the material storage mechanism, and a flow guiding mechanism is provided at the bottom of the sorting mechanism.

[0006] The flow guiding mechanism includes a mounting buckle, which is fixed to one side of the mounting bracket by bolts. A material guide hopper is fixed to the inner wall of the mounting buckle. A hydrophobic nano-coating is fixed to the inner wall of the material guide hopper. A flow guiding pipe is fixed to the bottom of the material guide hopper. A spiral flow guiding block is fixed to the inner wall of the flow guiding pipe. The spiral flow guiding block and the flow guiding pipe cooperate to form an Archimedean spiral flow channel. Multiple V-shaped flow guiding fins are fixed to the inner wall of the flow guiding pipe near the spiral flow guiding block. An airflow compensation mechanism is provided on the outer side of the flow guiding pipe.

[0007] As a further improvement of this utility model, a control panel is provided on one side of the mounting housing.

[0008] As a further embodiment of this utility model: the detection mechanism includes a lateral laser emitter, which is fixed to one side of the mounting cover by bolts. A collecting lens assembly is fixed to the side of the mounting cover opposite to the lateral laser emitter. A forward laser emitter is fixed to the side wall of the mounting cover. A dripping mechanism is provided on the top of the mounting cover.

[0009] As a further embodiment of this utility model: the storage mechanism includes a mounting base, which is fixed to one side of the mounting bracket by bolts. A storage cylinder is fixed to the top of the mounting base, and a filling port is fixed to the bottom of the storage cylinder.

[0010] As a further embodiment of this utility model: the sorting mechanism includes an installation pipe, the installation pipe is fixed to the bottom of the storage cylinder, a control valve is fixed to the bottom of the installation pipe, a connecting pipe is fixed to the bottom of the control valve, a sorting instrument is fixed to the bottom of the connecting pipe, and a drain pipe is fixed to the bottom of the sorting instrument.

[0011] As a further embodiment of this utility model: the airflow compensation mechanism includes an air pump bracket, which is fixed to one side of a mounting bracket by bolts. A miniature air pump is fixed to the inner wall of the air pump bracket. An air outlet pipe is fixed to one side of the miniature air pump. An air collection groove is fixed to one end of the air outlet pipe. Multiple connecting air pipes are fixed to the inner wall of the air collection groove. A solenoid valve is fixed to one end of each of the multiple connecting air pipes. An air delivery pipe is fixed to one side of the solenoid valve. An air nozzle is fixed to one end of the air delivery pipe. The air nozzle is fixedly connected to a guide pipe. A pair of air nozzles are fixed to the side wall of the guide pipe.

[0012] As a further embodiment of this utility model: the dripping mechanism includes a support frame, which is fixed to the top of the mounting cover by bolts. A sheath fluid storage tank is fixed to the inner wall of the support frame. A dripping pipe is fixed to the bottom of the sheath fluid storage tank. A top cover is fixed to the top of the sheath fluid storage tank. An inlet pipe is fixed to the top of the top cover. The inlet pipe is connected to a guide pipe.

[0013] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0014] 1. By incorporating a flow guiding mechanism and applying a hydrophobic nano-coating to create a superhydrophobic surface on the inner wall of the feed hopper, the adhesion rate of droplets on the inner wall of the feed hopper is reduced. After the sheath fluid enters the flow guide tube, it is guided by a spiral flow guide block. Centrifugal force is used to achieve secondary acceleration of the droplets. V-shaped flow guide fins are set in the flow channel abrupt change zone to disrupt the retention effect of the boundary layer during sheath fluid flow, effectively reduce eddy current intensity, and improve the uniformity of sheath fluid flow velocity. The combination of these mechanisms can effectively reduce sheath fluid residue, avoid cross-contamination, and eliminate the need for frequent shutdowns for cleaning, ensuring the continuity of flow cytometer use.

[0015] 2. When the sheath fluid flows to the outlet of the guide tube, the airflow compensation mechanism monitors the droplet flight trajectory in real time and sprays it in different directions. This allows for adjustment of the guide parameters of the guide mechanism and adaptation to changes in the viscosity and droplet velocity of different samples. It reduces the angle deviation of droplet impact, thereby reducing the risk of adhesion of droplets of different sizes and improving the stability of the guide operation. Attached Figure Description

[0016] Figure 1 A schematic diagram of an isometric structure according to an embodiment of the present invention is shown;

[0017] Figure 2 A schematic diagram of an isometric sectional view of a structure according to an embodiment of the present invention is shown;

[0018] Figure 3 The present invention provides an embodiment of the present invention. Figure 2 Enlarged structural diagram of part A in the middle;

[0019] Figure 4 The present invention provides an embodiment of the present invention. Figure 2 Enlarged structural diagram of section B in the middle;

[0020] Figure 5 A schematic diagram of the detection mechanism structure provided according to an embodiment of the present utility model is shown;

[0021] Figure 6 A schematic diagram of the flow guiding mechanism provided according to an embodiment of the present invention is shown.

[0022] Legend:

[0023] 100 Mounting housing, 110 Control panel, 120 Mounting cover, 130 Mounting bracket, 210 Side laser emitter, 220 Collecting lens assembly, 230 Forward laser emitter, 310 Mounting base, 320 Storage cylinder, 330 Filling port, 410 Mounting tube, 420 Control valve, 430 Connecting tube, 440 Sorting instrument, 450 Drain pipe, 510 Mounting clip, 520 Guide hopper, 521 Hydrophobic nano-coating, 530 Guide tube, 531 Spiral guide block, 540 V-shaped guide fin, 610 Air pump bracket, 620 Miniature air pump, 621 Air outlet pipe, 630 Air collection tank, 631 Connecting air pipe, 640 Solenoid valve, 650 Air delivery pipe, 660 Air nozzle, 670 High-precision infrared sensor, 710 Support frame, 720 Sheath fluid storage tank, 730 Drip pipe, 740 Top cover, 750 Inlet pipe. Detailed Implementation

[0024] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," and "top" are used interchangeably.

[0025] The orientation or positional relationship indicated by terms such as "bottom", "inner", "outer", "clockwise", and "counterclockwise" is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the purpose of facilitating the description of this utility model and simplifying the description, and is not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this utility model.

[0026] In the description of this utility model, "multiple" means two or more, unless otherwise explicitly specified.

[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "sleeved / connected," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0028] Please see Figure 1-6This utility model provides a technical solution: including a mounting housing 100, a control panel 110 is provided on one side of the mounting housing 100, a mounting cover 120 is fixed to the side wall of the mounting housing 100, a detection mechanism is provided on the outer side of the mounting cover 120, a mounting bracket 130 is fixed to the inner wall of the mounting housing 100 by bolts, a material storage mechanism is provided on the top of the mounting bracket 130, a sorting mechanism is provided at the bottom of the material storage mechanism, and a flow guiding mechanism is provided at the bottom of the sorting mechanism;

[0029] The flow guiding mechanism includes a mounting buckle 510, a feed hopper 520, a hydrophobic nano-coating 521, and a flow guiding tube 530. A spiral flow guiding block 531 is fixed to the inner wall of the flow guiding tube 530. The spiral flow guiding block 531 and the flow guiding tube 530 cooperate to form an Archimedean spiral flow channel. Multiple V-shaped flow guiding fins 540 are fixed to the inner wall of the flow guiding tube 530 near the spiral flow guiding block 531. An airflow compensation mechanism is provided on the outer side of the flow guiding tube 530. By providing the flow guiding mechanism and using the hydrophobic nano-coating 521 to form a superhydrophobic surface on the inner wall of the feed hopper 520, the adhesion rate of droplets on the inner wall of the feed hopper 520 can be reduced. After the sheath fluid enters the flow guiding tube 530, the spiral flow guiding block 531 guides the sheath fluid, utilizing centrifugal force to... The secondary acceleration of the droplets, achieved by incorporating V-shaped guide fins 540 in the abrupt change zone of the flow channel, disrupts the boundary layer retention effect during sheath fluid flow, effectively reduces eddy current intensity, and enhances the uniformity of sheath fluid flow velocity. This combined mechanism effectively reduces sheath fluid residue, preventing cross-contamination and eliminating the need for frequent shutdowns for cleaning, ensuring the continuity of flow cytometer operation. When the sheath fluid reaches the outlet of the guide tube 530, the airflow compensation mechanism monitors the droplet trajectory in real time and sprays it towards different droplet positions. This allows for adjustment of the guide parameters of the guide mechanism, adapting to variations in sample viscosity and droplet velocity, reducing droplet impact angle deviation, and consequently lowering the risk of adhesion from droplets of different sizes, thus improving the stability of the guide operation.

[0030] Specifically, the detection mechanism includes a lateral laser emitter 210, which is fixed to one side of the mounting cover 120 by bolts. A collecting lens assembly 220 is fixed to the side of the mounting cover 120 opposite to the lateral laser emitter 210. A forward laser emitter 230 is fixed to the side wall of the mounting cover 120. A dripping mechanism is provided on the top of the mounting cover 120. With the detection mechanism, the sheath fluid drips through the dripping mechanism, and the lateral laser emitter 210, the collecting lens assembly 220, and the forward laser emitter 230 work together to detect the sheath fluid.

[0031] Specifically, the storage mechanism includes a mounting base 310, which is fixed to one side of the mounting bracket 130 by bolts. A storage cylinder 320 is fixed to the top of the mounting base 310, and a filling port 330 is fixed to the bottom of the storage cylinder 320. By providing the storage mechanism, the sheath fluid is stored through the storage cylinder 320.

[0032] Specifically, the sorting mechanism includes an installation pipe 410, which is fixed to the bottom of the storage cylinder 320. A control valve 420 is fixed to the bottom of the installation pipe 410, a connecting pipe 430 is fixed to the bottom of the control valve 420, a sorting instrument 440 is fixed to the bottom of the connecting pipe 430, and a drain pipe 450 is fixed to the bottom of the sorting instrument 440. With the sorting mechanism, the sheath liquid inside the storage cylinder 320 enters the sorting instrument 440 through the installation pipe 410 and the connecting pipe 430. The sorting instrument 440 sorts the sheath liquid, and the sorted sheath liquid enters the guide hopper 520 through the drain pipe 450.

[0033] Specifically, the airflow compensation mechanism includes an air pump bracket 610, which is bolted to one side of the mounting bracket 130. A miniature air pump 620 is fixed to the inner wall of the air pump bracket 610. An air outlet pipe 621 is fixed to one side of the miniature air pump 620. An air collection groove 630 is fixed to one end of the air outlet pipe 621. Multiple connecting air pipes 631 are fixed to the inner wall of the air collection groove 630. A solenoid valve 640 is fixed to one end of each of the multiple connecting air pipes 631. An air delivery pipe 650 is fixed to one side of the solenoid valve 640. A jet nozzle 660 is fixed to one end of the air delivery pipe 650. The jet nozzle 660 is fixedly connected to the guide pipe 530. The side wall of the guide pipe 530 is fixed... A pair of jet nozzles 660 are provided; an airflow compensation mechanism is set up, and high-precision infrared sensors 670 on both sides monitor the flight trajectory of the droplets in real time and control the start and stop of multiple solenoid valves 640. A micro air pump 620 delivers gas to the inside of the gas collection tank 630 through the air outlet pipe 621, and finally sprays the gas out through the jet nozzles 660. Multiple jet nozzles 660 form a ring array of air curtain nozzles and are distributed around the guide outlet. By spraying the droplets in different directions through the jet nozzles 660, the guide parameters of the guide mechanism can be adjusted and adapted to the changes in viscosity and droplet velocity of different samples. This reduces the angle deviation of droplet impact, thereby reducing the adhesion risk of droplets of different sizes and improving the stability of the guide operation.

[0034] Specifically, the dripping mechanism includes a support frame 710, which is fixed to the top of the mounting cover 120 by bolts. A sheath fluid storage tank 720 is fixed to the inner wall of the support frame 710. A dripping pipe 730 is fixed to the bottom of the sheath fluid storage tank 720. A top cover 740 is fixed to the top of the sheath fluid storage tank 720. An inlet pipe 750 is fixed to the top of the top cover 740. The inlet pipe 750 is connected to the guide pipe 530. With the dripping mechanism, the sheath fluid enters the sheath fluid storage tank 720 through the inlet pipe 750 and drips downwards through the dripping pipe 730, which, in conjunction with the detection mechanism, performs detection operations on the sheath fluid.

[0035] Working Principle: During use, the device is controlled via the control panel 110. Sheath fluid is stored in the storage cylinder 320. The sheath fluid inside the storage cylinder 320 enters the sorting instrument 440 through the installation pipe 410 and connecting pipe 430. The sorting instrument 440 sorts the sheath fluid, and the sorted sheath fluid enters the guide hopper 520 through the drain pipe 450. The sheath fluid directly contacts the hydrophobic nano-coating 521, which forms a superhydrophobic surface on the inner wall of the guide hopper 520, reducing the adhesion rate of droplets on the inner wall. After entering the guide pipe 530, the sheath fluid is guided by the spiral guide block 531. Centrifugal force is used to achieve secondary acceleration of the droplets. V-shaped guide fins 540 are set in the flow channel abrupt change zone to disrupt the boundary layer retention effect during sheath fluid flow, effectively reduce eddy current intensity, and improve the uniformity of sheath fluid flow velocity. When the liquid flows to the outlet of the guide tube 530, the high-precision infrared sensors 670 on both sides monitor the flight trajectory of the droplets in real time and control the start and stop of multiple solenoid valves 640. The micro air pump 620 delivers the gas to the gas collection tank 630 through the air outlet pipe 621. The gas collection tank 630 delivers the gas to the gas delivery pipe 650 through the connecting air pipe 631. Finally, the gas is ejected through the jet nozzle 660. Multiple jet nozzles 660 form a ring array of air curtain nozzles and are distributed around the guide outlet. By spraying the droplets in different directions through the jet nozzles 660, the guide parameters of the guide mechanism can be adjusted and adapted to different sample viscosities and droplet velocity changes. The sheath fluid enters the sheath fluid storage tank 720 through the liquid inlet pipe 750 and drips downward through the drip pipe 730. The sheath fluid is detected by the side laser emitter 210, the collecting lens assembly 220 and the forward laser emitter 230.

[0036] Although the present invention discloses embodiments and accompanying drawings, those skilled in the art will understand that various substitutions, variations and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the contents disclosed in the embodiments and accompanying drawings.

Claims

1. A flow cytometer droplet sorting anti-adhesion and diversion device, characterized in that, The device includes a mounting housing (100), a mounting cover (120) fixed to the side wall of the mounting housing (100), a detection mechanism provided on the outer side of the mounting cover (120), a mounting bracket (130) fixed to the inner wall of the mounting housing (100) by bolts, a material storage mechanism provided on the top of the mounting bracket (130), a sorting mechanism provided on the bottom of the material storage mechanism, and a flow guiding mechanism provided on the bottom of the sorting mechanism. The flow guiding mechanism includes a mounting buckle (510), which is fixed to one side of the mounting bracket (130) by bolts. A guide hopper (520) is fixed to the inner wall of the mounting buckle (510). A hydrophobic nano-coating (521) is fixed to the inner wall of the guide hopper (520). A flow guiding pipe (530) is fixed to the bottom of the guide hopper (520). A spiral flow guiding block (531) is fixed to the inner wall of the flow guiding pipe (530). The spiral flow guiding block (531) and the flow guiding pipe (530) cooperate to form an Archimedes spiral flow channel. Multiple V-shaped flow guiding fins (540) are fixed to the inner wall of the flow guiding pipe (530) near the spiral flow guiding block (531). An airflow compensation mechanism is provided on the outer side of the flow guiding pipe (530).

2. The flow cytometer sort droplet anti-attachment flow directing device of claim 1, wherein, A control panel (110) is provided on one side of the mounting housing (100).

3. The flow cytometer sort droplet anti-attachment flow directing device of claim 1, wherein, The detection mechanism includes a lateral laser emitter (210), which is fixed to one side of a mounting cover (120) by bolts. A collecting lens assembly (220) is fixed to one side of the mounting cover (120) relative to the lateral laser emitter (210). A forward laser emitter (230) is fixed to the side wall of the mounting cover (120). A dripping mechanism is provided on the top of the mounting cover (120).

4. The flow cytometer sort droplet anti-attachment flow directing device of claim 1, wherein, The storage mechanism includes a mounting base (310), which is fixed to one side of the mounting bracket (130) by bolts. A storage cylinder (320) is fixed to the top of the mounting base (310), and a filling port (330) is fixed to the bottom of the storage cylinder (320).

5. The flow cytometer sort droplet anti-attachment flow directing device of claim 4, wherein, The sorting mechanism includes an installation pipe (410), which is fixed to the bottom of the storage cylinder (320). A control valve (420) is fixed to the bottom of the installation pipe (410), a connecting pipe (430) is fixed to the bottom of the control valve (420), a sorting instrument (440) is fixed to the bottom of the connecting pipe (430), and a drain pipe (450) is fixed to the bottom of the sorting instrument (440).

6. The flow cytometer sort droplet anti-attachment flow directing device of claim 1, wherein, The airflow compensation mechanism includes an air pump bracket (610), which is fixed to one side of the mounting bracket (130) by bolts. A miniature air pump (620) is fixed to the inner wall of the air pump bracket (610). An air outlet pipe (621) is fixed to one side of the miniature air pump (620). An air collection groove (630) is fixed to one end of the air outlet pipe (621). A plurality of connecting air pipes (631) are fixed to the inner wall of the air collection groove (630). A solenoid valve (640) is fixed to one end of each of the plurality of connecting air pipes (631). An air delivery pipe (650) is fixed to one side of the solenoid valve (640). A jet nozzle (660) is fixed to one end of the air delivery pipe (650). The jet nozzle (660) is fixedly connected to the guide pipe (530). A pair of jet nozzles (660) are fixed to the side wall of the guide pipe (530).

7. The flow cytometer sort droplet anti-attachment flow directing device of claim 3, wherein, The dripping mechanism includes a support frame (710), which is fixed to the top of the mounting cover (120) by bolts. A sheath fluid storage tank (720) is fixed to the inner wall of the support frame (710). A dripping pipe (730) is fixed to the bottom of the sheath fluid storage tank (720). A top cover (740) is fixed to the top of the sheath fluid storage tank (720). An inlet pipe (750) is fixed to the top of the top cover (740). The inlet pipe (750) is connected to the guide pipe (530).

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