Centrifugal screening device for monoclonal antibody

Abstract

The utility model relates to the technical field of monoclonal antibodies, and discloses a centrifugal screening device for monoclonal antibodies, which comprises a box body, a centrifugal component is arranged in the box body, an adjusting component is arranged in the centrifugal component, the centrifugal component comprises a shaft rod and a rotating frame, and the rotating frame is arranged on the shaft rod. The shaft rod is installed at the top end of an output shaft of the motor in the box body, the rotating frame is fixedly installed at the top end of the shaft rod, four supporting blocks are fixedly installed on the outer portion of the shaft rod, four limiting grooves are formed in the outer portion of the rotating frame, and a pipe sleeve is movably installed in each limiting groove. The test tubes can be automatically inclined through the tube sleeves and the positioning rods, so that cells of separated specific antibodies are positioned at the bottom ends of the test tubes, and the test tubes are prevented from colliding with the inner wall of the box body; through the screw rod and the base, the test tube support height can be conveniently adjusted, and test tubes with different lengths can be supported and fixed.

Description

A centrifugal screening device for monoclonal antibodies Technical Field

[0001] This utility model relates to the field of monoclonal antibody technology, specifically to a centrifugal screening device for monoclonal antibodies. Background Technology

[0002] Monoclonal antibodies are antibodies produced by a single type of immune cell and are highly specific and homogeneous. In the preparation process, spleen cells from an immunized animal are fused with myeloma cells to form hybridoma cells. Only a portion of these hybrid cells can produce the target monoclonal antibody. Due to the difference in density between the cells and the supernatant, the cells settle to the bottom of the centrifuge tube by centrifugation, while the supernatant is separated to isolate the cells that can produce the specific antibody.

[0003] The publication number CN220969492U discloses a centrifugal screening device for monoclonal antibodies, including a centrifuge chamber, a motor installed at the bottom of the centrifuge chamber, a turntable connected to the output shaft of the motor, and a support plate installed below the turntable.

[0004] The above-mentioned centrifugation screening device effectively increases the operating space for staff and facilitates the replacement of the elastic plate. However, during centrifugation, the separated cells precipitate on the inner wall of the test tube under gravity after centrifugation with the test tube tilted and fixed. After centrifugation with the test tube fixed vertically, the separated cells will also separate to the inner wall of the test tube, resulting in a large amount of mixing between the cells and the supernatant. It is not convenient to make the test tube automatically tilt during the separation process and automatically vertical after separation. At the same time, the test tubes of different specifications have different lengths, making it inconvenient to adjust the length of the test tube support and fixation. Summary of the Invention

[0005] The purpose of this invention is to provide a centrifugation screening device for monoclonal antibodies to solve the technical problems in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A centrifugal screening device for monoclonal antibodies includes a housing, inside which a centrifugal assembly is installed. Inside the centrifugal assembly is an adjusting assembly. The centrifugal assembly includes a shaft and a rotating frame. The shaft is mounted on the top of the output shaft of a motor inside the housing. The rotating frame is fixedly mounted on the top of the shaft. Four support blocks are fixedly mounted on the outside of the shaft. Four limiting grooves are formed on the outside of the rotating frame. A tube sleeve is movably installed inside each limiting groove. Positioning rods are fixedly mounted on the outside of the rotating shafts on both sides of the tube sleeve. Four sets of positioning blocks are fixedly mounted on the outside of the rotating frame.

[0008] Preferably, the four support blocks are fixedly surrounded around the outside of the shaft, and the direction of each support block corresponds to each limiting groove.

[0009] Preferably, the sleeve is located inside the limiting groove and is rotatably mounted via two rotating shafts on both sides, with the positioning rod and the sleeve both being perpendicular.

[0010] Preferably, the positioning rod is fixedly connected to the outer end of the rotating shaft of the sleeve, and there are two positioning blocks in each group.

[0011] Preferably, the adjustment assembly includes a base and two fixing blocks. The two fixing blocks are respectively fixedly installed on the outside of the tube sleeve and the base. A rotating sleeve is movably installed through the bottom of the fixing block. A fixing sleeve is fixedly installed at the bottom of the tube sleeve. A knob is fixedly installed at the top of the rotating sleeve. A screw is fixedly installed at the top of the fixing block outside the base. A telescopic rod is installed at the top of the base and through the inside of the fixing sleeve.

[0012] Preferably, the rotating sleeve is movably rotatable inside the fixed block, and the screw is located inside the rotating sleeve and is threadedly connected.

[0013] Preferably, the telescopic rod is fixedly installed with the base, and the telescopic rod is slidably connected inside the fixed sleeve.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1) This centrifugation screening device, by setting centrifugation components, the tube sleeve rotates and drives the positioning rod to swing, which can make the test tube automatically tilt, thereby placing the cells of the specific antibody separated at the bottom of the test tube, while improving the fixation of the test tube to prevent it from popping out. The rotation angle is limited by the positioning block to prevent the test tube from colliding with the inner wall of the chamber.

[0016] 2) This centrifugal screening device, by setting an adjustment component, rotates the sleeve to drive the screw to extend and retract, thereby raising and lowering the base. The base supports the bottom of the test tube, making it easy to adjust the height of the test tube support. It is suitable for supporting and fixing test tubes of different lengths. Attached Figure Description

[0017] Figure 1 is a schematic diagram of the overall structure of a centrifugal screening device for monoclonal antibodies according to an embodiment of the present invention;

[0018] Figure 2 is a schematic diagram of the centrifugal assembly in an embodiment of this utility model;

[0019] Figure 3 is a schematic diagram of the structure of the adjustment component in an embodiment of this utility model;

[0020] Figure 4 is an overall internal side view of an embodiment of this utility model.

[0021] In the diagram: 1. Box body; 2. Centrifugal assembly; 3. Adjustment assembly; 4. Shaft; 5. Support block; 6. Rotating frame; 7. Limiting groove; 8. Tube sleeve; 9. Positioning rod; 10. Positioning block; 11. Base; 12. Fixing block; 13. Rotating sleeve; 14. Fixing sleeve; 15. Knob; 16. Screw; 17. Telescopic rod. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Example 1

[0024] Referring to Figures 1-4, a centrifugation screening device for monoclonal antibodies includes a housing 1, a centrifugation assembly 2 installed inside the housing 1, and an adjustment assembly 3 installed inside the centrifugation assembly 2.

[0025] Referring to Figure 2, it is further seen that the centrifugal assembly 2 includes a shaft 4 and a rotating frame 6. The shaft 4 is installed at the top of the output shaft of the motor inside the housing 1. The rotating frame 6 is fixedly installed at the top of the shaft 4. Four support blocks 5 are fixedly installed on the outside of the shaft 4. Four limiting grooves 7 are opened on the outside of the rotating frame 6. A sleeve 8 is movably installed inside each limiting groove 7. Positioning rods 9 are fixedly installed on the outside of the rotating shafts on both sides of the sleeve 8. Four sets of positioning blocks 10 are fixedly installed on the outside of the rotating frame 6.

[0026] Four support blocks 5 are fixedly wrapped around the outside of the shaft 4. The direction of each support block 5 corresponds to each limiting groove 7. The test tube is inserted into the tube sleeve 8 and supported to a vertical state by the support blocks 5.

[0027] The sleeve 8 is located inside the limiting groove 7 and is movably rotated via two rotating shafts. The positioning rod 9 and the sleeve 8 are both in a vertical state. During centrifugation, the sleeve 8 causes the test tube to tilt automatically, thereby separating the cells of the specific antibody to the bottom of the test tube.

[0028] The positioning rod 9 is fixedly connected to the outer end of the rotating shaft of the sleeve 8. There are two positioning blocks 10 in each group. The positioning rod 9 rotates with the sleeve 8, and the rotation angle of the sleeve 8 is limited by the positioning blocks 10.

[0029] Specifically, the rotating frame 6 is driven to rotate by the shaft 4, and the test tube is inserted into the tube sleeve 8. During the rotation, the tube sleeve 8 automatically rotates and tilts under the action of centrifugal force at the bottom of the test tube, which can separate the cells of specific antibodies to the bottom of the test tube. At the same time, the positioning rod 9 rotates and contacts the fixing block 12 to limit the tilt angle.

[0030] Example 2

[0031] Referring to Figure 3, and based on Embodiment 1, the adjustment component 3 further includes a base 11 and two fixing blocks 12. The two fixing blocks 12 are respectively fixedly installed on the outside of the sleeve 8 and the base 11. A rotating sleeve 13 is movably installed through the bottom of the fixing block 12. A fixing sleeve 14 is fixedly installed at the bottom of the sleeve 8. A knob 15 is fixedly installed at the top of the rotating sleeve 13. A screw 16 is fixedly installed at the top of the fixing block 12 outside the base 11. A telescopic rod 17 is installed at the top of the base 11 and inside the fixing sleeve 14.

[0032] The rotating sleeve 13 is movably rotatable inside the fixed block 12. The screw 16 is located inside the rotating sleeve 13 and is threadedly connected. By rotating the rotating sleeve 13 through the knob 15, the screw 16 drives the base 11 to slide up and down under the action of the thread.

[0033] The telescopic rod 17 is fixedly installed with the base 11. The telescopic rod 17 is slidably connected inside the fixed sleeve 14. The telescopic rod 17 is used to prevent the base 11 from rotating. The extension and retraction of the telescopic rod 17 allows the base 11 to rise and fall smoothly.

[0034] Specifically, rotating the knob 15 causes the rotating sleeve 13 to rotate, and the screw 16 drives the base 11 to slide up and down under the action of the thread. The base 11 is raised and lowered smoothly through the telescopic rod 17. The height of the test tube is adjusted by raising and lowering the base 11.

[0035] In actual operation, the monoclonal antibody stock solution is added to the test tube and the test tube is placed in the rotating rack 6. The internal motor is controlled by the control system inside the box 1 to make the rotating rack 6 drive the test tube to rotate, and the cells that can produce specific antibodies are separated.

[0036] During centrifugation, the test tube is inserted and fixed inside the tube sleeve 8. The rotating frame 6 rotates through the shaft 4. As the bottom of the test tube rotates under the centrifugal action, the tube sleeve 8 rotates, causing the test tube to tilt automatically. This allows the cells containing the specific antibodies to be separated to be located at the bottom of the test tube, while also improving the fixation of the test tube and preventing it from popping out. When the test tube is tilted, the rotating shaft of the tube sleeve 8 drives the positioning rod 9 to swing. When the positioning rod 9 contacts the positioning block 10, the tube sleeve 8 tilts the test tube to a specified angle, preventing it from colliding with the inner wall of the chamber 1.

[0037] When adjusting the fixed height, different test tubes have different lengths. Rotating the knob 15 causes the rotating sleeve 13 to rotate. The screw 16 drives the base 11 to slide up and down through the rotating sleeve 13 and the thread. The base 11 is raised and lowered vertically through the telescopic rod 17 to avoid rotating with the rotating sleeve 13. The base 11 supports the bottom of the test tube.

[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A centrifugal screening device for monoclonal antibodies, comprising a housing (1), characterized in that: The centrifugal assembly (2) is installed inside the housing (1), and the centrifugal assembly (2) is installed inside the centrifugal assembly (2). The centrifugal assembly (2) includes a shaft (4) and a rotating frame (6). The shaft (4) is installed at the top of the output shaft of the motor inside the housing (1). The rotating frame (6) is fixedly installed at the top of the shaft (4). Four support blocks (5) are fixedly installed on the outside of the shaft (4). Four limiting grooves (7) are opened on the outside of the rotating frame (6). A sleeve (8) is movably installed inside each limiting groove (7). Positioning rods (9) are fixedly installed on the outside of the rotating shafts on both sides of the sleeve (8). Four sets of positioning blocks (10) are fixedly installed on the outside of the rotating frame (6).

2. The centrifugal screening device for monoclonal antibodies according to claim 1, characterized in that: The four support blocks (5) are fixedly surrounded around the outside of the shaft (4), and the direction of each support block (5) corresponds to each limiting groove (7).

3. The centrifugal screening device for monoclonal antibodies according to claim 1, characterized in that: The sleeve (8) is located inside the limiting groove (7) and is rotatably mounted on both sides of the rotating shaft. The positioning rod (9) and the sleeve (8) are both perpendicular to each other.

4. The centrifugal screening device for monoclonal antibodies according to claim 1, characterized in that: The positioning rod (9) is fixedly connected to the outer end of the rotating shaft of the sleeve (8), and there are two positioning blocks (10) in each group.

5. The centrifugal screening device for monoclonal antibodies according to claim 1, characterized in that: The adjustment assembly (3) includes a base (11) and two fixing blocks (12). The two fixing blocks (12) are respectively fixedly installed on the outside of the sleeve (8) and the base (11). A rotating sleeve (13) is movably installed through the bottom of the fixing block (12). A fixing sleeve (14) is fixedly installed at the bottom of the sleeve (8). A knob (15) is fixedly installed at the top of the rotating sleeve (13). A screw (16) is fixedly installed at the top of the fixing block (12) outside the base (11). A telescopic rod (17) is installed at the top of the base (11) and inside the fixing sleeve (14).

6. The centrifugal screening device for monoclonal antibodies according to claim 5, characterized in that: The rotating sleeve (13) is movably rotatable inside the fixed block (12), and the screw (16) is located inside the rotating sleeve (13) and is threadedly connected.

7. The centrifugal screening device for monoclonal antibodies according to claim 5, characterized in that: The telescopic rod (17) is fixedly installed with the base (11), and the telescopic rod (17) is slidably connected inside the fixed sleeve (14).

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