Cracking tube for field fast detection multi-index integrated DNA (Deoxyribose Nucleic Acid) kit

Abstract

The utility model provides a splitting tube for a field rapid detection multi-index integrated DNA (deoxyribonucleic acid) kit. The splitting tube comprises a splitting tube, a sealing cover and an integrated connecting device, the cracking tube is prepared from materials which can be kept stable under the heating condition of 95 DEG C, such as polypropylene or polycarbonate; the sealing cover is plugged at the pipe orifice of the cracking pipe in a sealing manner; and the integrated connecting device is sleeved on the adjacent cracking pipes, so that the plurality of cracking pipes are integrated. Technical breakthrough is achieved through structural innovation, and field fast detection efficiency and reliability are remarkably improved: the stepped sealing cover is attached to multiple sections of the cracking pipe to form a multi-stage sealing barrier, high-temperature-resistant materials and polypropylene / polycarbonate are matched, automatic suspension is achieved after the cover is opened, and risks of contact with a contaminated surface and cross contamination are avoided; the double-bulge limiting structure ensures that the fixing sleeve does not fall off, the cracking tube is made of a more stable material and does not interfere with the nucleic acid extraction process, and meanwhile, the multi-stage sealing structure can avoid cross infection.

Description

Technical Field

[0001] This invention belongs to the field of biological detection, specifically a lysis tube for a multi-index integrated DNA reagent kit for rapid field testing. Background Technology

[0002] In field rapid testing and resource-constrained scenarios, such as environmental monitoring, disease control, and ecological research, there is an urgent need for rapid extraction of DNA from biological samples and integrated detection of multiple indicators. However, traditional lysis devices suffer from multiple technical bottlenecks: their sealing structures are mostly single-layer planes or simple protruding joints, which are difficult to withstand the steam pressure generated by high-temperature lysis and the vibration during transportation, easily leading to leakage or cross-contamination of samples; the sealing cap and lysis tube are independently separated, and after opening, they are easy to fall off, be lost, or come into contact with contaminated surfaces, increasing operational risks; the lysis tubes are stored independently and lack modular integration design, which means that when processing multiple samples, repeated opening and closing and one-by-one operation are required, resulting in low efficiency and difficulty in ensuring the consistency of sample processing; at the same time, the existing lysis tube materials cannot balance high-temperature stability and structural elasticity. Rigid materials are prone to cracking under thermal stress, while elastic materials have insufficient sealing or are difficult to disassemble. Utility Model Content

[0003] To address the aforementioned technical problems, this invention provides a lysis tube for a multi-index integrated DNA reagent kit for rapid field testing. This solves the problem that existing lysis tube materials are difficult to balance high-temperature stability and structural elasticity, with rigid materials prone to cracking under thermal stress and elastic materials having insufficient sealing or being difficult to disassemble.

[0004] A lysis tube for a multi-indicator integrated DNA rapid detection kit for field use includes a lysis tube, a sealing cap, and an integrated connecting device;

[0005] The pyrolysis tube is made of a material such as polypropylene or polycarbonate that can remain stable under heating conditions of 95°C.

[0006] A sealing cap, a sealing plug, is placed at the opening of the pyrolysis tube;

[0007] An integrated connecting device is fitted onto adjacent pyrolysis tubes, making multiple pyrolysis tubes form a single unit.

[0008] Preferably, the sealing cap has an integral anti-detachment strip on its side, and the other end of the anti-detachment strip is connected to the pyrolysis tube.

[0009] Preferably, the sealing cap and the pyrolysis tube are connected by a stepped interface, and the sealing cap and the opening of the pyrolysis tube are fitted together.

[0010] Preferably, the surface of the pyrolysis tube is provided with two rings of protrusions, and the integrated connecting device is sleeved on the upper part of the pyrolysis tube and limited between the two rings of protrusions.

[0011] Preferably, the integrated connecting device includes a fixing sleeve and a connecting strip, the connecting strip and the fixing sleeve being integrally connected alternately in sequence, and the fixing sleeve and the connecting strip being made of plastic with a certain degree of elasticity.

[0012] Preferably, the integrated connecting device and the pyrolysis tube form a detachable structure.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. This utility model achieves a technological breakthrough through structural innovation, significantly improving the efficiency and reliability of rapid field testing: The stepped sealing cap and the multi-segment lysis tube form a multi-level sealing barrier, combined with high-temperature resistant materials, polypropylene / polycarbonate, reducing the risk of leakage under 95℃ lysis and violent shaking; the anti-detachment strip connects the sealing cap and the lysis tube in one piece, automatically suspending after opening to avoid contact with contaminated surfaces and the risk of cross-contamination; the fixed sleeve and connecting strip of the elastic integrated connection device achieve modular assembly through deformation, and each lysis tube can be disassembled independently, balancing integration and flexibility; the double-protrusion limiting structure ensures that the fixed sleeve will not fall off, and the lysis tube uses a more stable material that will not interfere with the nucleic acid extraction process, while the multi-level sealing structure can prevent cross-infection. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0016] Figure 2 This is a top view schematic diagram of the pyrolysis tube structure of this utility model;

[0017] Figure 3 This is a bottom-view three-dimensional structural diagram of the pyrolysis tube of this utility model;

[0018] Figure 4 This is a schematic diagram of the three-dimensional structure of the integrated connecting device of this utility model after assembly.

[0019] In the diagram: 1. Pyrolysis tube; 2. Sealing cap; 3. Anti-detachment strip; 4. Protrusion; 5. Fixing sleeve; 6. Connecting strip. Detailed Implementation

[0020] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0021] Example 1: As Figure 1-4 As shown, a lysis tube for a multi-index integrated DNA rapid detection kit for the field includes a lysis tube 1, a sealing cap 2, and an integrated connecting device.

[0022] The pyrolysis tube 1 is made of a material such as polypropylene or polycarbonate that can remain stable under heating conditions of 95°C.

[0023] The sealing cap 2 is a sealing plug placed at the opening of the pyrolysis tube 1. The sealing cap 2 and the pyrolysis tube 1 are connected by a stepped interface. The sealing cap 2 and the opening of the pyrolysis tube 1 are fitted together. The stepped interface of the sealing cap 2 can seal with multiple parts of the opening of the pyrolysis tube 1, thereby further ensuring the sealing performance of the pyrolysis tube 1.

[0024] An integrated connecting device is fitted onto adjacent pyrolysis tubes 1, so that multiple pyrolysis tubes 1 are integrated into one unit.

[0025] Example 2: Unlike Example 1, the sealing cap 2 is integrally provided with an anti-detachment strip 3 on its side. The other end of the anti-detachment strip 3 is connected to the pyrolysis tube 1. The anti-detachment strip 3 can catch the sealing cap 2 when it is opened, preventing the sealing cap 2 from falling off. It also prevents the sealing cap 2 from coming into contact with the table, thus preventing cross-contamination.

[0026] Example 3: Unlike Example 1, the integrated connecting device includes a fixing sleeve 5 and a connecting strip 6. The connecting strip 6 and the fixing sleeve 5 are connected in an alternating manner. The fixing sleeve 5 and the connecting strip 6 are made of plastic with a certain degree of elasticity. They have a certain degree of elasticity so that the pyrolysis tube 1 can be easily removed from the fixing sleeve 5. At the same time, when the fixing sleeve 5 is on the pyrolysis tube 1, the pyrolysis tube 1 will not easily fall off. The integrated connecting device and the pyrolysis tube 1 form a detachable structure.

[0027] Example 4: Unlike Examples 1 and 3, the surface of the pyrolysis tube 1 is provided with two rings of protrusions 4. The integrated connecting device is sleeved on the upper part of the pyrolysis tube 1 and limited between the two rings of protrusions 4. The fixing sleeve 5 is sleeved between the two rings of protrusions 4, which can fix the position of the fixing sleeve 5. Multiple pyrolysis tubes 1 can be connected together to form an integral unit, and each pyrolysis tube 1 can be processed in an independent state, which can be more convenient for subsequent independent disassembly.

[0028] The working principle of the lysis tube in this field rapid multi-index integrated DNA kit is as follows: The lysis tube 1 is made of a material such as polypropylene or polycarbonate that can remain stable under 95°C heating conditions. The sealing cap 2 fits tightly with the opening of the lysis tube 1 through its stepped mating interface, achieving multi-part sealing and ensuring the airtightness of the sample inside the lysis tube 1. In Example 2, an anti-detachment strip 3 is integrally set on the side of the sealing cap 2, and its other end is connected to the lysis tube 1. When the sealing cap 2 is opened, the anti-detachment strip 3 can catch the sealing cap 2 to prevent it from falling off, while avoiding contact between the sealing cap 2 and the table surface, which could lead to cross-contamination. In Example 3, the integrated connection device is composed of a fixing sleeve 5 and a connecting strip 6 that are sequentially and integrally connected, and the fixing sleeve 5 and the connecting strip 6 is made of elastic plastic and is fitted onto adjacent lysis tubes 1 by a fixing sleeve 5, so that multiple lysis tubes 1 are integrated. At the same time, the elastic design makes it easy to remove the lysis tubes 1 from the fixing sleeve 5, and the lysis tubes 1 are not easy to fall off after being fitted, forming a detachable structure. In embodiment four, the surface of the lysis tube 1 is provided with two rings of protrusions 4. When the fixing sleeve 5 of the integrated connecting device is fitted onto the lysis tube 1, it is partially limited between the two rings of protrusions 4, fixing the position of the fixing sleeve 5, thereby connecting multiple lysis tubes 1 into one unit while keeping each lysis tube 1 in an independent state, so as to facilitate independent disassembly later. This ensures that the lysis tubes 1 achieve safe sample storage, convenient operation and efficient integrated processing in the process of multi-index integrated DNA detection in field rapid testing.

[0029] The embodiments of this utility model are given for the purpose of illustration and description. Although embodiments of this utility model have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this utility model. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this utility model.

Claims

1. A lysis tube for a multi-index integrated DNA rapid detection kit for field use, characterized in that, It includes a pyrolysis tube (1), a sealing cap (2), and an integrated connecting device; The pyrolysis tube (1) is made of a material that can remain stable under heating conditions of 95°C, such as polypropylene or polycarbonate. A sealing cap (2) is placed at the opening of the pyrolysis tube (1); An integrated connecting device is fitted onto adjacent pyrolysis tubes (1) to form a single unit from multiple pyrolysis tubes (1).

2. The lysis tube for the multi-index integrated DNA rapid detection kit as described in claim 1, characterized in that: The sealing cap (2) is integrally provided with an anti-detachment strip (3) on its side, and the other end of the anti-detachment strip (3) is connected to the pyrolysis tube (1).

3. The lysis tube for the multi-index integrated DNA rapid detection kit as described in claim 1, characterized in that: The sealing cap (2) and the pyrolysis tube (1) are connected by a stepped interface, and the sealing cap (2) and the opening of the pyrolysis tube (1) are matched.

4. The lysis tube for the multi-index integrated DNA rapid detection kit as described in claim 1, characterized in that: The surface of the pyrolysis tube (1) is provided with two rings of protrusions (4), and the integrated connecting device is sleeved on the upper part of the pyrolysis tube (1) and limited between the two rings of protrusions (4).

5. The lysis tube for the multi-index integrated DNA rapid detection kit as described in claim 1, characterized in that: The integrated connecting device includes a fixing sleeve (5) and a connecting strip (6), which are connected in an alternating manner. The fixing sleeve (5) and the connecting strip (6) are made of plastic with a certain degree of elasticity.

6. The lysis tube for the multi-index integrated DNA rapid detection kit as described in claim 5, characterized in that: The integrated connecting device and the pyrolysis tube (1) form a detachable structure.

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