Detection device for density gradient centrifugal sample

Abstract

The utility model provides a detection device for a density gradient centrifugal sample, and belongs to the technical field of detection devices. The device comprises a sample suction platform mechanism which is used for sucking layered samples to be detected in a centrifugal tube layer by layer and conveying the layered samples to a sample detection chamber of an optical detector mechanism; the optical detector mechanism is provided with a xenon lamp light source and a beam shrinking light path system and supports 200-1100 nm full-wavelength detection; and the automatic collecting platform mechanism is used for sub-packaging the samples detected by the optical detector mechanism. According to the utility model, a separation system and a collection system are integrated into a continuous process, so that the man-made interference is reduced, and the resolution ratio and the efficiency are improved. The xenon lamp light source and the beam shrinking light path system are adopted, the detection precision and stability are improved based on the spectrophotometer principle, the detection wavelength can be freely selected, and the limitation of traditional dual wavelengths is broken through.

Description

Technical Field

[0001] This utility model relates to the field of detection device technology, and in particular to a detection device for density gradient centrifuged samples. Background Technology

[0002] Density gradient centrifugation is a fundamental and commonly used separation technique in biochemistry and life science research. It utilizes the differences in sedimentation coefficients of different substances to separate them into bands within regions of varying density gradients under specific centrifugal speeds or forces. Typical applications include the separation of lipoproteins of different densities, the purification and separation of large cell particles, the separation of nucleic acids, the separation of subcellular particles, and the separation of biological membranes. Commonly used density gradient preparation solutions include sucrose, cesium chloride, and sodium bromide. However, how to quickly, conveniently, and accurately collect our target substances from the different density bands formed after density gradient centrifugation remains a problem that needs to be solved.

[0003] Currently, there are three methods for collecting samples after density gradient centrifugation. The first is visual observation, using a needle to slowly pierce the liquid surface from above, reaching the target layer, and then manually aspirating the sample. The second is piercing the bottom of the centrifuge tube with a needle and collecting the liquid; later improvements can also control the flow rate for collection. However, both of these methods suffer from low resolution because the vertical movement of the gradient can leave particles on the centrifuge tube wall. The third method is Biocomp's fully automated density gradient preparation and separation system. This system solves the problem of particle residue on the centrifuge tube wall by moving a piston from top to bottom. It increases the resolution and accuracy of the collected samples by installing corresponding UV 260nm and 280nm wavelength LEDs to detect the collected sample signals. However, Biocomp currently only offers 260nm and 280nm light sources. While this system is very effective for collecting spectral substances with these wavelengths, such as nucleic acids and proteins, it still has significant limitations. First, the separation and collection systems are two separate systems. Second, the limitation to the two wavelengths means that there are no selectable signal acquisition methods for certain samples, such as liposomes and large cell particles. Utility Model Content

[0004] In view of this, to address the technical problem in existing fully automated density gradient preparation and separation systems where the separation and collection systems are two independent systems and only allow for the selection of two wavelengths, resulting in a lack of selectable signal acquisition methods for the collection of certain samples such as liposomes and large cell particles, this invention provides a detection device for density gradient centrifuged samples. By integrating the separation and collection systems into a continuous process, human interference is reduced, and resolution and efficiency are improved. By employing a xenon lamp light source and a beam-constricting optical path system, based on the principle of spectrophotometry, detection accuracy and stability are improved, and the detection wavelength can be freely selected, breaking through the limitations of traditional dual-wavelength methods.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A detection device for density gradient centrifuged samples, comprising:

[0007] The sample aspiration platform mechanism is used to aspirate the layered samples to be tested from the centrifuge tubes layer by layer and transport them to the sample detection chamber of the optical detector mechanism.

[0008] The optical detector mechanism is equipped with a xenon lamp source and a beam-contraction optical path system, supporting full wavelength detection from 200nm to 1100nm.

[0009] An automated collection platform mechanism is used to dispense samples that have been detected by the optical detector mechanism.

[0010] Preferably, the sample aspiration platform mechanism includes:

[0011] The base is mounted on the platform of the sample absorption platform mechanism;

[0012] A hydraulic support is mounted on the base;

[0013] The slide table is slidably connected to the hydraulic support;

[0014] A fixing sleeve is provided on the base for fixing the centrifuge tube;

[0015] The hydraulic rod has a hydraulic suction head at its lower end for aspirating the layered sample to be tested from the centrifuge tube, and its upper end is connected to the slide table.

[0016] A drive unit is used to drive the slide table to move up and down along the extension direction of the hydraulic support.

[0017] Preferably, the driving device includes:

[0018] A lead screw mechanism, which has a lead screw;

[0019] A hydraulic motor is connected to the lead screw via a coupling, and drives the slide table to raise and lower the hydraulic rod via the lead screw.

[0020] Preferably, the optical detector mechanism further includes:

[0021] A signal detector is used to collect the ultraviolet spectral absorption signal of the sample.

[0022] Preferably, the automated collection platform mechanism includes:

[0023] A collection needle is used to collect the sample after testing in the sample detection chamber into a sample collection plate.

[0024] Preferably, the automated collection platform mechanism further includes:

[0025] A drive system is used to drive the collection needle to move to the area where the sample collection plate is located.

[0026] Preferably, the driving system includes an X-axis driving system for driving the collecting needle to move along the X-axis direction and / or a Y-axis driving system for driving the collecting needle to move along the Y-axis direction.

[0027] Preferably, the X-axis drive system drives the Y-axis drive system to move along the X-axis direction, and the collecting needle is disposed on the Y-axis drive system.

[0028] Preferably, the automatic collection platform mechanism further includes a waste liquid tank.

[0029] Preferably, the automated collection platform mechanism further includes a heat sink.

[0030] Compared with the prior art, this utility model has the following beneficial effects:

[0031] This invention relates to a device for detecting samples by density gradient centrifugation. The unique feature of this device is its ingenious integration of the separation and collection systems into a continuous process, significantly reducing interference from human operation. This integrated design not only improves the resolution of the entire detection process but also greatly enhances work efficiency. Furthermore, the device employs a xenon lamp light source and a beam-constricting optical path system, based on the principles of a spectrophotometer, further enhancing the accuracy and stability of the detection. Users can freely select different detection wavelengths as needed, freeing the device from the limitations of traditional dual-wavelength methods and providing greater flexibility and higher accuracy for scientific research and experiments.

[0032] This invention, based on the integration of separation and collection systems, innovatively combines the principle of a spectrophotometer. By using a beam-constricted optical path, it enables the detection of flowing samples, improving the method of detecting flowing liquid paths. By integrating the separation and collection systems into one system and utilizing an ultraviolet-full-wavelength detection optical path, it is possible to select any wavelength within the visible light wavelength range for detection, or to perform a full-wavelength scan of the liquid path to determine the characteristic absorption of substances. This makes the collection method after density gradient centrifugation accurate, fast, and visualized.

[0033] Other advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0035] Figure 2 This is a schematic diagram of the sample aspiration platform mechanism of this utility model;

[0036] Figure 3 This is a schematic diagram of the optical detector mechanism of this utility model;

[0037] Figure 4 This is a schematic diagram of the automatic sample collection platform mechanism of this utility model;

[0038] Figure 5 To Figure 3 A schematic diagram of the detection optical path in the sample detection chamber of an optical detector;

[0039] In the diagram, 1. Sample aspiration platform mechanism; 2. Optical detector mechanism; 3. Automatic sample collection platform mechanism; 11. Hydraulic support; 12. Screw mechanism; 121. Screw; 122. Support end; 123. Fixed end; 13. Slide table; 14. First connecting flange; 15. Hydraulic rod; 151. Hydraulic suction head; 16. Coupling; 17. Fixed sleeve; 18. Hydraulic motor; 19. Hydraulic motor mounting plate; 20. Guide rail; 21. Xenon lamp light source; 22. 1. Beam-shrinking optical path system; 23. Sample detection chamber; 24. Signal detector; 31. Y-axis motor; 311. Y-axis motor shaft; 32. Y-axis guide rail; 33. First X-axis guide rail; 34. Second connecting flange; 35. X-axis motor; 351. X-axis motor shaft; 36. Second X-axis guide rail; 37. Mounting plate; 38. Connecting rod; 39. Collection needle; 3010. Waste liquid pool; 3011. Sample collection plate; 3012. Radiator; 3013. Fixing sleeve. Detailed Implementation

[0040] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0041] In the description of this utility model, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0042] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "sleeved / connected," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0043] like Figure 1 As shown, this utility model provides a detection device for density gradient centrifuged samples, comprising:

[0044] The sample aspiration platform mechanism 1 is used to aspirate the layered samples to be tested from the centrifuge tubes layer by layer and transport them to the sample detection chamber 23 of the optical detector mechanism 2. The main function of the sample aspiration platform mechanism 1 is to automate the aspiration and transport of samples. It can accurately aspirate the layered samples to be tested from the centrifuge tubes layer by layer and then transport these samples to the sample detection chamber 23 of the optical detector mechanism 2 for further analysis and detection. This process greatly reduces the tediousness and errors of manual operation, and improves experimental efficiency and accuracy.

[0045] The optical detector mechanism 2 is equipped with a xenon lamp source 21 and a beam-constricting optical path system 22, supporting full-wavelength detection from 200nm to 1100nm. It covers the ultraviolet (characteristic absorption of nucleic acids and proteins), visible light (scattering of liposomes and cell clusters), and near-infrared (detection of specific markers) spectral ranges. Based on the principle of a spectrophotometer, it uses a beam-constricting optical path to detect flowing samples, improving the method of detecting flowing liquids. It integrates the separation and collection systems into one system. Utilizing the ultraviolet-full-wavelength detection optical path, it allows for the selection of any wavelength within the visible light range for detection, or it can perform a full-wavelength scan of the liquid path to determine the characteristic absorption of substances. This makes the collection method after density gradient centrifugation accurate, rapid, and visualized.

[0046] The xenon lamp light source 21 emits a continuous spectrum that penetrates the liquid path of the flow sample detection chamber 23. The signal detector captures the transmitted light signal in real time and calculates the absorbance or the intensity of the scattered light.

[0047] When light of a specific wavelength passes through a flowing sample, the absorption intensity of the target substance is proportional to its concentration (formula: A = ε·c·l, where A is absorbance, ε is molar absorptivity, c is concentration, and l is optical path length).

[0048] Among them, the beam-shrinking optical path system 22, such as Figure 5 As shown. The xenon lamp light source 21 utilizes the broadband composite light emitted by the xenon lamp. The beam-constricting optical path is based on a dispersive beam splitter to achieve wavelength separation of the beam, forming a reference optical path and a sample optical path that are parallel vertically. The beam first passes through the objective lens for beam constriction and then enters the eyepiece for focusing. The reference optical path passes through the air, while the sample optical path penetrates the flowing sample under test. Finally, the two sets of optical paths, carrying optical information of different wavelengths, arrive at the detector simultaneously. By comparing the attenuation or change characteristics of specific wavelengths of light in the two paths, the optical performance of the sample is analyzed. Here, the reference optical path is assumed to detect air, and the sample optical path detects the density gradient layered solution absorbed by the sample absorption platform mechanism 1.

[0049] Therefore, this invention solves the three major technical bottlenecks in density gradient centrifugation sample detection—namely, single wavelength, low sensitivity, and fragmented operation—by modifying the spectrophotometer principle, designing a precise optical path for the narrow beam, and integrating the functions of the entire system. It achieves broad-spectrum, high-precision, and fully automated detection and collection, providing an efficient and reliable tool for biochemical separation experiments.

[0050] An automated collection platform mechanism is used to dispense samples after they have been detected by the optical detector mechanism 2. This effectively organizes and manages the samples, ensuring that each sample is processed according to a predetermined procedure, thereby improving the efficiency and accuracy of the entire detection process.

[0051] like Figure 2As shown, in this utility model, the sample aspiration platform mechanism 1 includes:

[0052] The base is set on the platform of the sample aspiration platform mechanism 1, and serves as the supporting foundation for the sample aspiration platform mechanism 1, the optical detector mechanism 2, and the automatic collection platform mechanism.

[0053] The hydraulic support 11 is mounted on the base and supports the rigid frame of the entire sample suction platform mechanism 1, and is fixedly connected to the base.

[0054] The slide 13 is slidably connected to the hydraulic support 11, and the slide 13 is preferably slidably connected to the slide rail provided on the hydraulic support 11.

[0055] The fixing sleeve 17 is set on the base to fix the centrifuge tube, ensuring that the sample tube is vertical and stable during the aspiration process and avoiding tilting or shaking of the liquid surface.

[0056] The hydraulic rod 15 has a carefully designed lower end equipped with a hydraulic pipette tip 151 capable of aspirating the layered sample to be tested from the centrifuge tube. This hydraulic pipette tip 151 can accurately aspirate the sample, ensuring its integrity. The upper end of the hydraulic rod 15 is tightly connected to the slide table 13 (preferably via a first connecting flange 14), ensuring the stability and precise movement of the hydraulic rod 15. Furthermore, the hydraulic rod 15 employs a hollow tube design, which allows it to effectively transport the aspirated layered sample to be tested to the sample testing chamber 23. To further improve the efficiency and accuracy of sample transfer, the inner wall of the hollow tube is specially treated with a smooth material, such as a polytetrafluoroethylene coating, which significantly reduces liquid residue during transfer, ensuring sample purity and the accuracy of the test results.

[0057] In designing the hydraulic suction tip 151, its tip portion was specially considered to ensure optimal performance and safety. Typically, the tip is designed with a blunt shape, the primary purpose of which is to avoid disrupting the layered interfaces during puncture. Layered interfaces refer to the boundaries between different layers in certain liquids or substances, which need to be kept clear to avoid mixing or contamination. The blunt tip design effectively reduces damage to these interfaces, thus maintaining their integrity. Furthermore, the hydraulic suction tip 151 can be equipped with a miniature pressure sensor that monitors pressure changes in real time during aspiration. This monitoring function is crucial for preventing over-absorption of liquid, as excessive aspiration can cause mixing of substances that should remain layered, affecting experimental results or product quality. The built-in miniature pressure sensor provides timely pressure data, allowing the operator to adjust the aspiration force accordingly, ensuring the aspiration process is both effective and safe.

[0058] The drive unit is designed to drive the slide table 13 to move up and down along the extension direction of the hydraulic support 11. In this way, the hydraulic rod 15 and the hydraulic suction head 151 can be effectively driven to move up and down together, thereby realizing the suction of the layered sample to be tested.

[0059] In this utility model, the driving device includes:

[0060] The lead screw mechanism 12 has a lead screw 121, and may also have a support end 122 and a fixed end 123, such as... Figure 2 In the process, the hydraulic motor is mounted on the hydraulic motor mounting plate 19. The lead screw mechanism 12 includes a lead screw 121, a support end 122, a fixed end 123, and a lead screw nut. One end of the lead screw is mounted on the fixed end 123 to fix the lead screw, and the other end is mounted on the support end 122 to support the lead screw. The support end and the fixed end are fixedly mounted on the hydraulic support 11. The hydraulic motor is connected to the lead screw through a coupling 16. The lead screw nut is fixedly connected to the slide table 13. The slide table 13 is mounted on the guide rail 20. The hydraulic motor 18 drives the lead screw to rotate, which drives the lead screw nut on the lead screw to move up and down, thereby driving the entire slide table to move up and down along the guide rail 20.

[0061] A hydraulic motor 18 is tightly connected to the lead screw 121 via a coupling 16, and then drives the slide 13 through the lead screw 121, enabling the hydraulic rod 15 to move up and down. The hydraulic motor 18 is preferably fixed to the base. In this process, the hydraulic motor 18 serves as the main power source, and the coupling 16 above it plays a crucial connecting role. The connection between the coupling 16 and the lead screw 121 allows the lead screw 121 to rotate smoothly under the drive of the hydraulic motor 18. The rotation of the lead screw 121 is then converted into linear movement of the slide 13, thereby realizing the raising and lowering of the hydraulic rod 15.

[0062] like Figure 3 As shown, in this utility model, the optical detector mechanism 2 further includes:

[0063] The signal detector is used to collect the ultraviolet spectral absorption signal of the sample. It is an integral part of the optical detector mechanism 2 and is specifically designed to collect the spectral absorption signal generated by the sample after irradiation with ultraviolet light of a specific wavelength. By accurately detecting and analyzing these signals, the composition, concentration, and other relevant characteristics of the sample can be accurately determined and measured.

[0064] The optical detector mechanism 2 is as follows Figure 4 As shown, in this utility model, the automatic collection platform mechanism includes:

[0065] A collecting needle 39 is used to collect the tested sample from the sample detection chamber 23 into the sample collecting plate 3011. The sample collecting plate 3011 is a porous sample collecting plate 3011. The collecting needle 39 collects the tested sample from the sample detection chamber 23 and dispenses it into designated wells on the sample collecting plate 3011. This collecting needle 39 is particularly suitable for porous sample collecting plates 3011. During sample testing, the collecting needle 39 can effectively remove the tested sample from the detection chamber and ensure that the sample is accurately distributed into the pre-set wells on the sample collecting plate 3011, thereby achieving orderly sample management and subsequent analysis.

[0066] In this utility model, the automatic collection platform mechanism further includes:

[0067] A drive system is used to move the collection needle 39 to the area where the sample collection plate 3011 is located. This drive system is responsible for precise control and operation to move the collection needle 39 to the area where the sample collection plate 3011 is located, ensuring that the collection needle 39 can accurately reach the predetermined position, thereby achieving accurate sample collection.

[0068] In this invention, the drive system includes an X-axis drive system for driving the collecting needle 39 to move along the X-axis and / or a Y-axis drive system for driving the collecting needle 39 to move along the Y-axis. These two drive systems work together to ensure that the collecting needle 39 can be efficiently and accurately positioned and moved on a two-dimensional plane. The design and implementation of the X-axis and Y-axis drive systems enable the entire drive system to meet the requirements of high precision and high efficiency, thus playing a key role in automated equipment and improving production efficiency and product quality.

[0069] In this invention, the described X-axis drive system is responsible for driving the Y-axis drive system to move along the X-axis direction. Specifically, the Y-axis drive system is designed to be precisely positioned and moved along the X-axis direction to ensure it operates according to a predetermined path and speed. Furthermore, to achieve precise sample collection, a collection needle 39 is cleverly positioned and fixed to the Y-axis drive system. This arrangement allows the collection needle 39 to move synchronously with the movement of the Y-axis drive system, thereby achieving precise sample collection in the X-axis direction.

[0070] like Figure 4 As shown, in this invention, a bracket is formed by connecting rod 38 and mounting plate 37 for mounting the X-axis drive system. The X-axis drive system uses X-axis motor 35 as a power source, and its output end is connected to X-axis motor shaft 351 (such as an X-axis lead screw). A first X-axis guide rail 33 and a second X-axis guide rail 36 are respectively provided on both sides to provide sliding guide rails for the Y-axis drive system.

[0071] The Y-axis drive system has a slider that is slidably connected to a guide rail on the X-axis. A Y-axis motor is mounted on the slider, and the output end of the Y-axis motor is connected to a signal detector on the Y-axis motor axis 24. One end of a Y-axis lead screw (such as a Y-axis lead screw) is slidably connected to a second connecting flange 34, and a collecting needle 39 is mounted on the second connecting flange 34. The Y-axis drive system can drive the collecting needle 39 to move along the Y-axis guide rail 32 on the Y-axis via the Y-axis lead screw.

[0072] In this invention, the automated collection platform mechanism also includes a waste liquid tank 3010. During dispensing intervals or sample changes, the collection needle 39 discharges non-target liquid into the waste liquid tank 3010, preventing contamination of the sample collection plate 3011. This solves the core problems of inefficient waste liquid treatment and high pollution risk in traditional collection methods, significantly improving the reliability and safety of the fully automated detection-collection system, and providing key technical support for the standardization and large-scale application of biomedical experiments.

[0073] In this invention, the automatic heat collection platform mechanism also includes a radiator 3012, whose main function is to rapidly dissipate heat to ensure the stable operation of the entire platform and extend the service life of the equipment. The radiator 3012 is designed with heat conduction efficiency in mind and is typically made of high thermal conductivity materials, such as aluminum alloy or copper, to achieve optimal heat dissipation. Furthermore, the radiator 3012 may be equipped with a fan or other cooling device to enhance airflow and further improve heat dissipation performance. In some advanced applications, the radiator 3012 may also integrate an intelligent temperature control system that can automatically adjust the cooling intensity based on the heat generated during platform operation, thereby ensuring both performance and energy efficiency.

[0074] The installation and usage methods of this utility model are described below with reference to specific embodiments:

[0075] like Figure 1 As shown, this device is primarily composed of three main mechanisms: the sample aspiration platform mechanism 1, the optical detector mechanism 2, and the automatic sample collection platform mechanism 3. See details below. Figure 1 .

[0076] like Figure 2The diagram shows a schematic of the sample aspiration platform mechanism 1 of this device. First, the sample tube after density gradient centrifugation is placed in the fixing sleeve 17 and fixed. The fixing sleeve 17 is fixed on the fixing sleeve 3013 of the base. A coupling 16 is set above the hydraulic motor 18. The lead screw of the lead screw mechanism 12 is connected to the hydraulic motor 18 through the coupling 16. Then, during the hydraulic process, the lead screw mechanism 12 is driven to move downward under the action of the hydraulic motor 18. At the same time, a connecting flange is connected to the lead screw mechanism 12. The upper end is provided with a support end 122 and the lower end is provided with a fixing end 123. The connecting flange is connected to the hydraulic rod 15. The lower end of the hydraulic rod 15 is provided with a hydraulic suction head 151. Under the action of the hydraulic motor 18, the hydraulic suction head 151 is driven to move downward. The layered sample in the sample tube is sucked out layer by layer through the hydraulic suction head 151 and the hydraulic rod 15 into the sample detection chamber 23 of the optical detector mechanism 2.

[0077] like Figure 3 The diagram shows the optical detector mechanism 2 of this device. Under the action of the sample aspiration platform mechanism 1, the sample, after density gradient centrifugation, flows into the sample detection cell in layers. Simultaneously, the xenon lamp light source 21 passes through the beam-constricting optical path system 22, and according to the different ultraviolet absorption properties of the samples being detected, ... Figure 5 In the schematic diagram of the beam-contraction detection optical path shown, the sample can be detected at any wavelength between 200nm and 1100nm. The ultraviolet absorption signal of the sample is collected by the signal detector. After passing through the sample detection cell, the sample then enters the automatic sample collection platform mechanism 3.

[0078] like Figure 4 The diagram shows the automatic sample collection platform mechanism 3 of this device. The pressure motor 18 drives the pressure rod downwards via a lifting mechanism. Under pressure, the sample in the centrifuge tube passes through the light source detection system and the collection device along the collection tube in the horn head, finally reaching the position of the collection needle 39. The sample is collected into the sample collection plate 3011 according to volume or number of dispensing tubes. The collection needle 39, driven by the X-axis motor 35 and the Y-axis motor 31, can move along the X-axis guide rail and Y-axis guide rail 32 respectively. The movement of the collection needle 39 dispenses the sample into the corresponding holes in the sample collection plate 3011. A heat sink 3012 is provided below the automatic sample collection platform mechanism 3 to dissipate heat from the entire device.

[0079] The above description is merely a preferred embodiment of this utility model; however, the protection scope of this utility model is not limited thereto; any equivalent substitutions or modifications made by those skilled in the art within the technical scope disclosed in this utility model, based on the technical solution and its improved concept, should be covered within the protection scope of this utility model.

Claims

1. A detection device for density gradient centrifuged samples, characterized in that, include: The sample aspiration platform mechanism is used to aspirate the layered samples to be tested from the centrifuge tubes layer by layer and transport them to the sample detection chamber of the optical detector mechanism. The optical detector mechanism is equipped with a xenon lamp source and a beam-contraction optical path system, supporting full wavelength detection from 200nm to 1100nm. An automated collection platform mechanism is used to dispense samples that have been detected by the optical detector mechanism.

2. The detection device for density gradient centrifuged samples according to claim 1, characterized in that, The sample aspiration platform mechanism includes: The base is mounted on the platform of the sample absorption platform mechanism; A hydraulic support is mounted on the base; The slide table is slidably connected to the hydraulic support; A fixing sleeve is provided on the base for fixing the centrifuge tube; The hydraulic rod has a hydraulic suction head at its lower end for aspirating the layered sample to be tested from the centrifuge tube, and its upper end is connected to the slide table. A drive unit is used to drive the slide table to move up and down along the extension direction of the hydraulic support.

3. The detection device for density gradient centrifuged samples according to claim 2, characterized in that, The driving device includes: A lead screw mechanism, which has a lead screw; A hydraulic motor is connected to the lead screw via a coupling, and the lead screw drives the slide table to raise and lower the hydraulic rod.

4. The detection device for density gradient centrifuged samples according to claim 1, characterized in that, The optical detector mechanism further includes: A signal detector is used to collect the ultraviolet spectral absorption signal of the sample.

5. The detection device for density gradient centrifuged samples according to claim 1, characterized in that, The automated collection platform includes: The collection needle is used to collect the sample after testing in the sample detection chamber into the sample collection plate.

6. The detection device for density gradient centrifuged samples according to claim 5, characterized in that, The automated collection platform mechanism also includes: A drive system is used to drive the collection needle to move to the area where the sample collection plate is located.

7. The detection device for density gradient centrifuged samples according to claim 6, characterized in that, The drive system includes an X-axis drive system for driving the collecting needle to move along the X-axis direction and / or a Y-axis drive system for driving the collecting needle to move along the Y-axis direction.

8. The detection device for density gradient centrifuged samples according to claim 7, characterized in that, The X-axis drive system drives the Y-axis drive system to move along the X-axis direction, and the collecting needle is disposed on the Y-axis drive system.

9. The detection device for density gradient centrifuged samples according to claim 1, characterized in that, The automated collection platform also includes a waste liquid tank.

10. A detection device for density gradient centrifuged samples according to any one of claims 1-9, characterized in that, The automated collection platform mechanism also includes a radiator.

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