Stem cell preparation storing and transferring device

Abstract

The utility model relates to the field of cell preservation and transfer, and discloses a stem cell preparation preservation and transfer device which comprises an outer shell, an inner heat preservation shell is fixedly installed in the outer shell, a fixing base is fixedly installed in the inner heat preservation shell, a plurality of round limiting grooves are formed in the top face of the fixing base, and a plurality of through holes are formed in the round limiting grooves. A plurality of circular limiting grooves are formed in the outer shell, flexible sponge protective sleeves are fixedly installed in the multiple circular limiting grooves, stem cell preparation freezing pipes are arranged in the middles of the flexible sponge protective sleeves, a liquid nitrogen adding assembly is arranged on the side of the outer shell, and a protective plate body is arranged at the position, located above the fixing base, in the inner heat preservation shell. According to the device, through the combination of the protection plate body, the heat preservation flexible plate, the external threaded rod and the sealing protruding block, the overall heat preservation performance can be further enhanced, it is ensured that the freezing pipe cannot be directly exposed when the sealing heat preservation box cover is opened, heat entering is reduced as much as possible when a person takes a single freezing pipe, and the relative stability of the internal low-temperature environment is maintained.

Description

Technical Field

[0001] This application belongs to the field of cell preservation and transport technology, specifically a stem cell preparation preservation and transport device. Background Technology

[0002] Stem cell preparations, as biological products containing stem cells with self-replication and multi-directional differentiation potential, encompass different types such as hematopoietic stem cells and mesenchymal stem cells depending on their source and purpose. They are often present in the form of cell suspensions or cell tissue blocks. Their preservation and transportation are of great significance for maintaining activity and efficacy. For short-term transportation (within a few hours), low-temperature preservation at 2-8°C is used, with the cell activity ensured by transport culture medium containing nutrients, and temperature is stabilized by an incubator to avoid violent shaking. For long-term preservation and transportation, deep cryopreservation in liquid nitrogen (-196°C) is used, with strict temperature control and a sterile environment throughout the process to maintain the biological activity of stem cell preparations and ensure the effectiveness of clinical applications.

[0003] For example, utility model patent CN222357120U discloses a cryopreservation box for stem cells, including a box body, an internal placement slot containing several sets of inner liners, each inner liner having a columnar structure, and a liquid nitrogen freezing chamber inside the box body with an inlet at its upper part and a sealing cap at the inlet. This utility model's design, with its internal placement slot containing several sets of inner liners in a columnar structure, facilitates the placement of stem cell test tubes. The liquid nitrogen freezing chamber inside the box body with an inlet at its upper part and a sealing cap at the inlet facilitates the addition of liquid nitrogen, allowing for timely addition of liquid nitrogen during transport, thus ensuring the cryopreservation time.

[0004] However, during actual use, it was discovered that the device achieves long-term transportation by placing stem cell test tubes in an inner liner and freezing them with liquid nitrogen.

[0005] However, when the lid of the device is opened to take out or put in stem cell test tubes, the test tubes inside the box are exposed to the external environment, causing the temperature inside the box to rise rapidly. This makes it difficult to stabilize the low-temperature environment, and the stem cell preparations in the stem cell test tubes are affected by temperature fluctuations in a short period of time, resulting in reduced cell activity and poor cryopreservation effect. Therefore, a stem cell preparation preservation and transportation device is provided. Utility Model Content

[0006] The purpose of this application is to provide a stem cell preparation preservation and transport device in order to solve the problems mentioned above.

[0007] The technical solution adopted in this application is as follows: A stem cell preparation preservation and transport device includes an outer shell, an inner insulation shell fixedly installed inside the outer shell, a fixing seat fixedly installed inside the inner insulation shell, a plurality of circular limiting grooves opened on the top surface of the fixing seat, and a flexible sponge sheath fixedly installed inside each of the plurality of circular limiting grooves, a stem cell preparation cryotube disposed in the middle of the flexible sponge sheath, a liquid nitrogen addition component disposed on the side of the outer shell, a protective plate disposed above the fixing seat inside the inner insulation shell, a rectangular groove opened on the bottom surface of the protective plate, an insulation flexible plate fixedly installed inside the rectangular groove, a plurality of circular holes corresponding to the stem cell preparation cryotubes opened on the bottom surface of the insulation flexible plate, a plurality of internally threaded holes communicating with the circular holes opened on the top surface of the protective plate, an externally threaded rod connected to the internal thread of the internally threaded hole, a sealing protrusion fixedly installed at the bottom end of the externally threaded rod, and a limiting mechanism connected to the protective plate disposed inside the inner insulation shell.

[0008] In a preferred embodiment, the limiting mechanism includes a mounting groove, a limiting protrusion, and a spring. Two mounting grooves are symmetrically formed on the inner sidewall of the inner insulation shell, and a limiting protrusion is provided inside each of the two mounting grooves. Two springs are fixedly mounted on one end of the limiting protrusion, and the ends of the two springs away from the limiting protrusion are fixed to the inner sidewall of the mounting groove. An arc-shaped transition surface is provided on the end of the limiting protrusion away from the spring.

[0009] In a preferred embodiment, a sealed insulated box lid is hinged to the top surface of the outer shell, and a handle is fixedly installed on the top surface of the sealed insulated box lid.

[0010] In a preferred embodiment, a plurality of positioning posts are fixedly installed on the top surface of the fixing base near the corner, and a plurality of positioning holes corresponding to the positioning posts are opened on the bottom surface of the protective plate near the corner, and the positioning posts are inserted into the positioning holes.

[0011] In a preferred embodiment, a sign is fixedly installed on the top surface of the protective plate, located on the side of the internal threaded hole.

[0012] In a preferred embodiment, an anti-slip rubber pad is fixedly installed on the bottom surface of the outer casing.

[0013] In summary, due to the adoption of the above technical solution, the beneficial effects of this application are:

[0014] 1. In this application, due to the adoption of the above-mentioned solution, the device can form a tight insulation structure through the outer shell, the inner insulation shell, and the sealed insulation box lid, which greatly reduces heat exchange, effectively improves the insulation effect, and ensures that the inside of the inner insulation shell is kept at a low temperature for a long time, thus providing a guarantee for the safe transport of stem cell preparation cryotubes. At the same time, the combination of the protective plate, the insulating flexible plate, the external threaded rod, and the sealing protrusion can further enhance the overall insulation performance, ensuring that the cryotubes are not directly exposed when the sealed insulation box lid is opened, so that when personnel take out a single cryotube, heat is minimized and the internal low temperature environment is kept relatively stable. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this application;

[0016] Figure 2 This is a schematic diagram of the internal insulation shell structure of this application;

[0017] Figure 3 This is a partial exploded view of the structure of this application;

[0018] Figure 4 This is a schematic diagram of the thermal insulation flexible plate structure of this application;

[0019] Figure 5 For the purposes of this application Figure 3 Enlarged structural diagram at point A in the middle;

[0020] Figure 6 For the purposes of this application Figure 3 Enlarged structural diagram at point B;

[0021] Figure 7 This is a schematic diagram of the structure of the limiting protrusion plate in this application before it is installed.

[0022] The markings in the diagram are: 1. Outer shell; 2. Inner insulation shell; 3. Fixing base; 4. Circular limiting groove; 5. Flexible sponge sheath; 6. Stem cell preparation cryotube; 7. Protective plate; 8. Rectangular groove; 9. Insulating flexible plate; 10. Circular hole; 11. Internal threaded hole; 12. External threaded rod; 13. Sealing protrusion; 14. Liquid nitrogen addition component; 15. Limiting mechanism; 1501. Mounting groove; 1502. Limiting protrusion; 1503. Spring; 16. Sealed insulation box lid; 17. Handle; 18. Positioning post; 19. Positioning hole; 20. Identification plate; 21. Anti-slip rubber pad. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below in conjunction with the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0024] refer to Figures 1-7 As shown, a stem cell preparation preservation and transport device includes an outer shell 1, an inner insulating shell 2 fixedly installed inside the outer shell 1, a sealed insulating box lid 16 hinged to the top surface of the outer shell 1, a handle 17 fixedly installed on the top surface of the sealed insulating box lid 16, and an anti-slip rubber pad 21 fixedly installed on the bottom surface of the outer shell 1. The outer shell 1 and the inner insulating shell 2 can form a stem cell preparation preservation and transport box. An insulating layer is provided between the outer shell 1 and the inner insulating shell 2. The insulating layer can be vacuum-sealed or have an insulating board installed, which can improve the insulation performance of the transport box. The sealed insulating box lid 16 can effectively seal the transport box and prevent heat exchange. The handle 17 facilitates the opening and closing of the transport box and makes it easy to carry. The anti-slip rubber pad 21 makes the transport box more stable when placed and prevents it from sliding.

[0025] refer to Figures 1-7 As shown, a fixing seat 3 is fixedly installed inside the inner insulation shell 2. The top surface of the fixing seat 3 has multiple circular limiting grooves 4, and a flexible sponge sleeve 5 is fixedly installed inside each of the multiple circular limiting grooves 4. A stem cell preparation cryotube 6 is set in the middle of the flexible sponge sleeve 5, and a liquid nitrogen adding component 14 is set on the side of the outer shell 1. The fixing seat 3 and the circular limiting grooves 4 facilitate the positioning of the stem cell preparation cryotube 6, while the flexible sponge sleeve 5 can play a role in limiting and buffering protection to prevent the cryotube 6 from being damaged by collision during transportation. The liquid nitrogen adding component 14 can add and replenish liquid nitrogen into the inner insulation shell 2 to maintain a low temperature environment, which is conducive to the preservation of stem cell preparations.

[0026] The liquid nitrogen addition assembly 14 consists of a pipe connector, a filter, a protective housing, a connecting spray pipe, and a safety valve. The pipe connector and filter are located inside the outer protective housing of the outer shell 1 to effectively prevent collision damage. The pipe connector is used to connect to the external liquid nitrogen addition pipe, and the filter can filter out impurities in the liquid nitrogen. The filtered liquid nitrogen is evenly sprayed into the area below the fixing seat 3 inside the inner insulation housing 2 through the connecting spray pipe, thereby creating a low-temperature environment inside the inner insulation housing 2 and providing suitable conditions for the preservation and transportation of stem cell preparations. The safety valve is also installed inside the protective housing on one side of the outer shell 1. When the pressure exceeds the limit due to the addition of liquid nitrogen inside the inner insulation housing 2, it can release pressure in time to improve the safety of the device operation. The above components are all common and commonly used structures in reality and have a practical application basis. Although not all of them are shown in the attached drawings, it does not affect their functional realization and the overall operation of the device.

[0027] refer to Figures 1-7 As shown, a protective plate 7 is installed inside the inner insulation shell 2 above the fixing base 3. Multiple positioning posts 18 are fixedly installed on the top surface of the fixing base 3 near the corners. Multiple positioning holes 19 corresponding to the positioning posts 18 are opened on the bottom surface of the protective plate 7 near the corners. The positioning posts 18 are inserted into the positioning holes 19. A rectangular groove 8 is opened on the bottom surface of the protective plate 7. An insulating flexible plate 9 is fixedly installed inside the rectangular groove 8. Multiple circular holes 10 corresponding to the stem cell preparation cryotubes 6 are opened on the bottom surface of the insulating flexible plate 9. Through the cooperation of the positioning posts 18 and positioning holes 19, the protective plate 7 can be accurately positioned and stably installed. The insulating flexible plate 9 further enhances the insulation effect. At the same time, its circular holes 10 can be adapted to the cryotubes, playing a certain role in protection and positioning.

[0028] refer to Figure 1-7 As shown, the top surface of the protective plate 7 has multiple internally threaded holes 11 that communicate with the circular holes 10. A label 20 is fixedly installed on the side of the internally threaded holes 11 on the top surface of the protective plate 7. An externally threaded rod 12 is connected to the internal thread of the internally threaded hole 11. A sealing protrusion 13 is fixedly installed at the bottom end of the externally threaded rod 12. A limiting mechanism 15 connected to the protective plate 7 is provided inside the inner insulation shell 2. By cooperating with the internally threaded holes 11 and the externally threaded rod 12, the position of the sealing protrusion 13 can be adjusted by rotating the externally threaded rod 12 to achieve sealing of the top of the cryotube. The label 20 facilitates the labeling and identification of relevant information of different stem cell preparation cryotubes 6, so that subsequent personnel can take the designated stem cell preparation cryotube 6. The limiting mechanism 15 can restrict the position of the protective plate 7, making its installation more stable.

[0029] refer to Figures 1-7As shown, the limiting mechanism 15 includes a mounting groove 1501, a limiting protrusion 1502, and a spring 1503. Two mounting grooves 1501 are symmetrically opened on the inner sidewall of the inner insulation shell 2, and a limiting protrusion 1502 is provided inside each of the two mounting grooves 1501. Two springs 1503 are fixedly installed on one end of the limiting protrusion 1502, and the ends of the two springs 1503 away from the limiting protrusion 1502 are fixed to the inner sidewall of the mounting groove 1501. An arc-shaped transition surface is provided on the end of the limiting protrusion 1502 away from the springs 1503. The springs 1503 can provide elastic support force, so that the limiting protrusion 1502 can limit the protective plate 7. The arc-shaped transition surface facilitates the cooperation between the protective plate 7 and the limiting protrusion 1502 during installation, making the installation operation convenient.

[0030] The implementation principle of the stem cell preparation preservation and transportation device of this application is as follows: First, the personnel insert the stem cell preparation cryotube 6 into the flexible sponge sleeve 5. The flexible sponge sleeve 5, through its own softness, can not only accurately limit the position of the cryotube, but also effectively buffer the external impact force during transportation to avoid damage to the cryotube. Then, through the cooperation of the positioning post 18 on the top surface of the fixed seat 3 and the positioning hole 19 on the bottom surface of the protective plate 7, the protective plate 7 is stably installed above the fixed seat 3. The insulating flexible plate 9 further enhances the heat preservation performance. When the protective plate 7 is installed in place, the limiting protrusion 1502 in the limiting mechanism 15, under the elastic action of the spring 1503, limits the protective plate 7 to ensure that it will not shift during transportation, thereby maintaining the stable state of the stem cell preparation cryotube 6.

[0031] Then, the user connects to the liquid nitrogen adding component 14 through an external liquid nitrogen pipeline to add liquid nitrogen into the device, thereby maintaining the low temperature environment inside the inner insulation shell 2 and providing suitable conditions for the preservation of stem cell preparations. When a single stem cell preparation cryotube 6 needs to be retrieved, it can be quickly located according to the label 20, and the corresponding external thread rod 12 can be unscrewed to retrieve the cryotube. If all cryotubes need to be retrieved at once, the user can press the limiting protrusion 1502 to release the restriction on the protective plate 7, and then retrieve them conveniently and quickly. The device can form a tight insulation structure through the outer shell 1, the inner insulation shell 2 and the sealed insulation box cover 16, which greatly reduces heat exchange and effectively improves the insulation effect, ensuring that the inside of the inner insulation shell 2 is kept at a low temperature for a long time, providing a solid guarantee for the safe transport of stem cell preparation cryotubes 6. At the same time, the combined design of the protective plate 7, the insulating flexible plate 9, the external thread rod 12 and the sealing protrusion 13 further enhances the overall insulation performance, so that when the user retrieves a single cryotube, heat is minimized and the internal low temperature environment is kept relatively stable.

[0032] The above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A stem cell preparation preservation and transport device, comprising an outer shell (1), characterized in that: An inner insulation shell (2) is fixedly installed inside the outer shell (1). A fixing seat (3) is fixedly installed inside the inner insulation shell (2). A plurality of circular limiting grooves (4) are opened on the top surface of the fixing seat (3), and a flexible sponge sheath (5) is fixedly installed inside each of the plurality of circular limiting grooves (4). A stem cell preparation cryotube (6) is provided in the middle of the flexible sponge sheath (5). A liquid nitrogen addition component (14) is provided on the side of the outer shell (1). A protective plate (7) is provided inside the inner insulation shell (2) above the fixing seat (3). The bottom surface of the protective plate (7) A rectangular groove (8) is provided, and a thermal insulation plate (9) is fixedly installed inside the rectangular groove (8). The bottom surface of the thermal insulation plate (9) is provided with multiple circular holes (10) corresponding to the stem cell preparation cryotube (6). The top surface of the protective plate (7) is provided with multiple internal threaded holes (11) that communicate with the circular holes (10). An external threaded rod (12) is threaded inside the internal threaded hole (11). A sealing protrusion (13) is fixedly installed at the bottom end of the external threaded rod (12). A limiting mechanism (15) connected to the protective plate (7) is provided inside the inner thermal insulation shell (2).

2. The stem cell preparation preservation and transport device as described in claim 1, characterized in that: The limiting mechanism (15) includes a mounting groove (1501), a limiting protrusion (1502), and a spring (1503). The inner wall of the inner insulation shell (2) has two symmetrical mounting grooves (1501), and the interior of each of the two mounting grooves (1501) is provided with a limiting protrusion (1502). Two springs (1503) are fixedly installed at one end of the limiting protrusion (1502), and the ends of the two springs (1503) away from the limiting protrusion (1502) are fixed to the inner wall of the mounting groove (1501). The end of the limiting protrusion (1502) away from the springs (1503) is provided with an arc-shaped transition surface.

3. The stem cell preparation preservation and transport device as described in claim 1, characterized in that: The top surface of the outer shell (1) is hinged with a sealed insulated box cover (16), and a handle (17) is fixedly installed on the top surface of the sealed insulated box cover (16).

4. The stem cell preparation preservation and transport device as described in claim 1, characterized in that: Multiple positioning posts (18) are fixedly installed on the top surface of the fixed base (3) near the corner. Multiple positioning holes (19) corresponding to the positioning posts (18) are opened on the bottom surface of the protective plate (7) near the corner. The positioning posts (18) are inserted into the positioning holes (19).

5. The stem cell preparation preservation and transport device as described in claim 1, characterized in that: A sign (20) is fixedly installed on the top surface of the protective plate (7) on the side of the internal threaded hole (11).

6. The stem cell preparation preservation and transport device as described in claim 1, characterized in that: The bottom surface of the outer shell (1) is fixedly equipped with an anti-slip rubber pad (21).

Images