Fluorescence signal reading assembly
By designing a fluorescence signal reading component consisting of a simple filter and a reading box, the problems of complex operation and high cost of existing equipment have been solved, realizing low-cost and simple operation of fluorescence signal reading, which is suitable for economically underdeveloped areas.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHENZHEN UNIV
- Filing Date
- 2025-04-21
- Publication Date
- 2026-05-08
AI Technical Summary
Existing fluorescence signal reading devices, such as fluorescence microscopes, are complex to operate, require a high level of expertise, and are expensive, making them difficult to popularize in economically underdeveloped regions.
A fluorescence signal reading assembly consisting of a filter and a reading box was designed. By vertically setting the placement through-hole, excitation through-hole, and imaging through-hole, fluorescence signal reading can be achieved with simple operation using an external excitation light source and an imager, adapting to centrifuge tubes of different specifications.
It enables low-cost and simple operation of fluorescence signal reading, reduces the professional requirements for operators, is suitable for economically underdeveloped areas, and improves the accessibility of the equipment.
Smart Images
Figure CN224216556U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of optical signal reading technology, and in particular to a fluorescence signal reading component. Background Technology
[0002] Fluorescence, also known as light emission, is the light emitted by a substance after absorbing light or other electromagnetic radiation; it is a cold light emission phenomenon. In detection technologies such as medical testing, fluorescent groups are often used as reporter molecules; for example, in the field of molecular diagnostics, nucleic acid fluorescent probes emit fluorescence when the target gene is amplified.
[0003] Current fluorescence signal readings are typically achieved using fluorescence microscopes. However, reading fluorescence signals requires specialized operation by the operator, who must adjust various complex parameters within the microscope to obtain a good fluorescence signal image. This demands a high level of expertise from the operator. Furthermore, the high cost of fluorescence microscopes limits their widespread adoption in economically underdeveloped regions. Utility Model Content
[0004] The purpose of this invention is to provide a fluorescence signal reading component, which has a simple structure, low cost, and is easy to operate, requiring no professional expertise from the operator.
[0005] To achieve this objective, the present invention adopts the following technical solution:
[0006] A fluorescence signal reading component, used to read the fluorescence signal of a sample in a centrifuge tube, includes:
[0007] Filters;
[0008] The reading box has a side wall with a spaced-out through hole, an excitation through hole and a shooting through hole. The center lines of the spaced-out through hole, the excitation through hole and the shooting through hole are perpendicular to each other and intersect at the same point. The filter is detachably mounted on the reading box corresponding to the shooting through hole.
[0009] The centrifuge tube is placed in the reading box through the placement through-hole. When the excitation light source shines on the centrifuge tube through the excitation through-hole, the camera can capture and read the fluorescence signal of the sample in the centrifuge tube through the imaging through-hole.
[0010] As a further technical solution, the shooting through hole is set as an elongated hole extending along the Z direction, and the reading box is adjustablely provided with a shooting auxiliary component, and the shooting auxiliary component is provided with a shooting auxiliary hole corresponding to the shooting through hole;
[0011] The excitation through hole is configured as an elongated hole extending along the Z direction. The reading box is adjustablely provided with an excitation auxiliary component, and the excitation auxiliary component is provided with an excitation auxiliary hole corresponding to the excitation through hole.
[0012] As a further technical solution, the shooting auxiliary component is configured as a long strip plate corresponding to the shooting through hole, and can be detachably abutted against the inner wall of the shooting through hole;
[0013] The shooting aid is provided with one shooting aid hole, or, along the Z direction, the shooting aid is provided with multiple obstructable shooting aid holes at intervals.
[0014] As a further technical solution, the reading box is provided with a shooting auxiliary chamber extending in the Z direction corresponding to the shooting through hole, the shooting auxiliary component is a flexible plate and is movably installed in the shooting auxiliary chamber, and the shooting auxiliary component is provided with a shooting auxiliary hole.
[0015] As a further technical solution, the excitation auxiliary component is configured as a long strip plate corresponding to the excitation through hole, and can be detachably abutted against the inner wall of the excitation through hole;
[0016] The excitation aid is provided with one excitation aid hole, or, along the Z direction, the excitation aid is provided with multiple shieldable excitation aid holes at intervals.
[0017] As a further technical solution, the reading box is provided with an excitation auxiliary chamber extending in the Z direction corresponding to the excitation through hole, the excitation auxiliary component is provided as a flexible plate and is movably installed in the excitation auxiliary chamber, and the excitation auxiliary component is provided with an excitation auxiliary hole.
[0018] As a further technical solution, the cross-sectional area of the excitation auxiliary hole gradually increases from the excitation through hole to the center of the reading box.
[0019] As a further technical solution, the fluorescence signal reading component also includes a cover plate, which is disposed on the outer wall of the reading box corresponding to the placement through hole.
[0020] As a further technical solution, the fluorescence signal reading component also includes a guide and limiting component, and the cover plate is movably disposed on the outer wall of the reading box through the guide and limiting component.
[0021] As a further technical solution, a storage groove is also provided on the outer wall of the reading box. The storage groove is concentrically arranged with the storage through hole and cooperates with the storage through hole to form a limiting step.
[0022] And / or, an elastic limiting ring is fixedly provided on the inner side of the storage through hole.
[0023] Compared with the prior art, the fluorescent signal reading component provided by this utility model has the following technical advantages:
[0024] Since the fluorescence signal reading component consists only of a filter and a reading box, its overall structure is simple and inexpensive, making it suitable for widespread use in economically underdeveloped areas. To read the fluorescence signal, a centrifuge tube containing the sample is first inserted into the reading box through the placement port. An external excitation light source is then used to irradiate the centrifuge tube through the excitation port to excite the fluorescence signal of the sample inside. Because the reading box has a filter corresponding to the imaging port, the filter can filter out the excitation light emitted by the external excitation light source while retaining the fluorescence signal. Therefore, the operator can directly use an external imager to capture and read the fluorescence signal of the sample inside the centrifuge tube through the imaging port, using only the filter. The entire process is simple and requires no specialized skills from the operator. Attached Figure Description
[0025] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments of this utility model will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the content of the embodiments of this utility model and these drawings without creative effort.
[0026] Figure 1 This is a schematic diagram of the structure of the fluorescence signal reading component provided in this embodiment of the utility model;
[0027] Figure 2 This is a cross-sectional view of the fluorescence signal reading component provided in this embodiment of the present invention for capturing through-holes;
[0028] Figure 3 This is a cross-sectional view of the fluorescence signal reading component provided in this embodiment of the present invention with respect to the excitation via;
[0029] Figure 4 This is a cross-sectional view of the fluorescence signal reading component provided in this embodiment of the present invention with respect to a glass slide;
[0030] Figure 5 This is a cross-sectional view of the fluorescence signal reading component provided in this embodiment of the present invention for the test strip.
[0031] In the picture:
[0032] 10. Centrifuge tubes; 20. Glass slides; 30. Test paper;
[0033] 100. Filters;
[0034] 200. Reading box; 210. Storage through hole; 220. Excitation through hole; 221. Excitation auxiliary chamber; 230. Imaging through hole; 231. Imaging auxiliary chamber; 240. Storage groove; 250. Auxiliary reading plate; 251. Hook; 260. Auxiliary clip;
[0035] 300. Shooting aid; 310. Shooting aid hole;
[0036] 400. Excitation auxiliary component; 410. Excitation auxiliary hole;
[0037] 500, cover plate;
[0038] 600, guide and limit assembly; 610, limit post; 620, limit groove. Detailed Implementation
[0039] Before explaining any implementation of this application in detail, it should be understood that this application is not limited to its application to the structural details and component arrangements set forth in the following description or shown in the above drawings.
[0040] In this application, the terms "comprising," "including," "having," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.
[0041] In this application, the term "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent three cases: A existing alone, A and B existing simultaneously, and B existing alone. Additionally, the character " / " in this application generally indicates that the preceding and following related objects have an "and / or" relationship.
[0042] In this application, the terms "connection," "combination," "coupling," and "installation" can refer to direct connection, combination, coupling, or installation, or indirect connection, combination, coupling, or installation. For example, a direct connection refers to two parts or components being connected together without the need for an intermediary, while an indirect connection refers to two parts or components each being connected to at least one intermediary, with the connection achieved through the intermediary. Furthermore, "connection" and "coupling" are not limited to physical or mechanical connections or couplings, but can also include electrical connections or couplings.
[0043] In this application, those skilled in the art will understand that relative terms (e.g., “about,” “approximately,” “basically,” etc.) used in conjunction with quantities or conditions are to include the values and have the meaning indicated by the context. For example, such relative terms include at least the degree of error associated with the measurement of a particular value, tolerances associated with the particular value due to manufacturing, assembly, use, etc. Such terms should also be considered as disclosing a range defined by the absolute values of the two endpoints. Relative terms may refer to a certain percentage (e.g., 1%, 5%, 10% or more) of the indicated value. Numerical values not using relative terms should also be disclosed as specific values with tolerances. Furthermore, “basically” when expressing relative angular relationships (e.g., substantially parallel, substantially perpendicular) may refer to a certain degree (e.g., 1 degree, 5 degrees, 10 degrees or more) added to or subtracted from the indicated angle.
[0044] In this application, those skilled in the art will understand that the function performed by a component can be performed by one component, multiple components, one part, or multiple parts. Similarly, the function performed by a part can also be performed by one part, one component, or a combination of multiple parts.
[0045] In this application, the directional terms "upper," "lower," "left," "right," "front," and "rear" are used to describe the orientation and positional relationships shown in the accompanying drawings and should not be construed as limiting the embodiments of this application. Furthermore, in the context, it should be understood that when an element is mentioned as being connected "upper" or "lower" to another element, it can be directly connected to the other element "upper" or "lower," or indirectly connected through an intermediate element. It should also be understood that directional terms such as upper side, lower side, left side, right side, front side, and rear side not only represent positive orientation but can also be understood as lateral orientation. For example, "below" can include directly below, lower left, lower right, lower front, and lower rear.
[0046] Combination Figures 1 to 5 As shown, the fluorescence signal reading component provided in this embodiment is used to read the fluorescence signal of the sample in the centrifuge tube 10. Specifically, the fluorescence signal reading component includes a filter 100 and a reading box 200: the side wall of the reading box 200 is provided with a placement through hole 210, an excitation through hole 220 and an imaging through hole 230 at intervals. The midlines of the placement through hole 210, the excitation through hole 220 and the imaging through hole 230 are arranged perpendicularly to each other and intersect at the same point. The filter 100 is detachably installed in the reading box 200 corresponding to the imaging through hole 230. The centrifuge tube 10 is placed in the reading box 200 through the placement through hole 210. When the excitation light source shines on the centrifuge tube 10 through the excitation through hole 220, the imager can capture and read the fluorescence signal of the sample in the centrifuge tube 10 through the imaging through hole 230.
[0047] Since the fluorescence signal reading component consists only of a filter 100 and a reading box 200, its overall structure is simple and inexpensive, making it suitable for widespread use in economically underdeveloped areas. When reading the fluorescence signal, the centrifuge tube 10 containing the sample is first inserted into the reading box 200 through the placement through-hole 210. Then, an external excitation light source is used to irradiate the centrifuge tube 10 through the excitation through-hole 220 to excite the fluorescence signal of the sample inside the centrifuge tube 10. Because the reading box 200 has a filter 100 corresponding to the imaging through-hole 230, the filter 100 can filter out the excitation light emitted by the external excitation light source while retaining the fluorescence signal. Therefore, the operator can directly use an external camera to capture and read the fluorescence signal of the sample inside the centrifuge tube 10 through the imaging through-hole 230 using the filter 100. The entire process is simple and requires no professional expertise from the operator.
[0048] Furthermore, since the filter 100 is detachably mounted on the reading box 200, different specifications of the filter 100 can be used depending on the type of excitation light source, thereby further improving the applicability of the fluorescence signal reading component. The reading box 200 is configured as an opaque rectangular box, and the storage through hole 210 is located on the top wall of the reading box 200.
[0049] The excitation light source and the camera can be selected according to the actual operation. In this embodiment, the excitation light source is an LED light and the camera is a mobile phone.
[0050] Preferably, the imaging through hole 230 is configured as an elongated hole extending along the Z direction, and the reading box 200 is adjustablely provided with an imaging auxiliary component 300, which has an imaging auxiliary hole 310 corresponding to the imaging through hole 230; the excitation through hole 220 is configured as an elongated hole extending along the Z direction, and the reading box 200 is adjustablely provided with an excitation auxiliary component 400, which has an excitation auxiliary hole 410 corresponding to the excitation through hole 220.
[0051] Specifically, when the length of the centrifuge tube 10 inserted into the reading box 200 through the placement through-hole 210 changes, the excitation auxiliary component 400 can be adaptively adjusted according to the actual length of the centrifuge tube 10. This changes the relative position of the excitation auxiliary hole 410 and the excitation through-hole 220 on the excitation auxiliary component 400, so that the light from the external excitation light source illuminating the centrifuge tube 10 through the excitation auxiliary hole 410 is exactly at the center of the sample inside the centrifuge tube 10, thus ensuring the excitation effect on the fluorescence signal of the sample inside the centrifuge tube 10. At the same time, the imaging auxiliary component 300 is adaptively adjusted accordingly to the excitation auxiliary component 400, thereby changing the relative position of the imaging auxiliary hole 310 and the imaging through-hole 230 on the imaging auxiliary component 300. This ensures that when the imager captures and reads the fluorescence signal through the imaging auxiliary hole 310, the imaging center is directly opposite the center of the sample inside the centrifuge tube 10, thus ensuring the imaging and reading effect on the fluorescence signal of the sample inside the centrifuge tube 10. That is, by adaptively adjusting the imaging aid 300 and the excitation aid 400, the center lines of the imaging aid hole 310, the excitation aid hole 410 and the imaging through hole 230 are set perpendicular to each other and always intersect at the same point, thereby enabling the reading of fluorescence signals of samples in centrifuge tubes of different sizes, thereby improving the applicability of the fluorescence signal reading component.
[0052] This embodiment provides three methods for adjusting the relative position of the excitation auxiliary hole 410 and the excitation through hole 220, as follows:
[0053] In the first type, the excitation auxiliary component 400 is configured as a long strip corresponding to the excitation through hole 220 and is detachably abutted against the inner wall of the excitation through hole 220. The excitation auxiliary component 400 has one excitation auxiliary hole 410. Specifically, multiple excitation auxiliary components 400 are provided, each with only one excitation auxiliary hole 410, and the position of the excitation auxiliary hole 410 on each excitation auxiliary component 400 is different. When the specifications of the centrifuge tube 10 inserted into the reading box 200 change, it is only necessary to remove the original excitation auxiliary component 400 and then detach the excitation auxiliary component 400 with the corresponding excitation auxiliary hole 410 and abut against the inner wall of the excitation through hole 220. For example, when the target centrifuge tube 10 is long, the excitation auxiliary component 400 with the excitation auxiliary hole 410 closer to the bottom wall of the reading box 200 is selected; when the target centrifuge tube 10 is short, the excitation auxiliary component 400 with the excitation auxiliary hole 410 closer to the top wall of the reading box 200 is selected.
[0054] The second type involves an excitation auxiliary component 400 configured as a long strip corresponding to the excitation through-hole 220, which is detachably abutted against the inner wall of the excitation through-hole 220. Each excitation auxiliary component 400 has one such component, and multiple obstructable excitation auxiliary holes 410 are spaced apart along the Z-direction. When the specifications of the centrifuge tube 10 inserted into the reading box 200 change, simply obstruct the original excitation auxiliary hole 410 and then unobstruct the corresponding excitation auxiliary hole 410. For example, when the target centrifuge tube 10 is long, the corresponding camera auxiliary hole near the bottom wall of the reading box 200 is unobstructed; when the target centrifuge tube 10 is short, the corresponding camera auxiliary hole near the top wall of the reading box 200 is unobstructed.
[0055] The third type involves a reading box 200 having an excitation auxiliary chamber 221 extending along the Z direction corresponding to the excitation through-hole 220. The excitation auxiliary component 400 is a flexible plate and is movably installed within the excitation auxiliary chamber 221. The excitation auxiliary component 400 has an excitation auxiliary hole 410. Specifically, in conjunction with... Figure 3 As shown, the side wall of the reading box 200, which has an excitation through hole 220, has an excitation auxiliary chamber 221 extending along the Z direction, corresponding to the excitation through hole 220, so that a sandwich is formed in the middle of the side wall. The flexible excitation auxiliary member 400 is movably disposed in the sandwich, and an excitation auxiliary hole 410 is provided in the middle of the flexible excitation auxiliary member 400. When the specifications of the centrifuge tube 10 inserted in the reading box 200 change, the flexible excitation auxiliary member 400 can be manually moved so that it moves along the Z direction in the excitation auxiliary chamber 221, thereby changing the relative position of the excitation auxiliary hole 410 and the excitation through hole 220. For example, when the target centrifuge tube 10 is long, the excitation auxiliary member 400 is moved towards the bottom wall of the reading box 200; when the target centrifuge tube 10 is short, the excitation auxiliary member 400 is moved towards the top wall of the reading box 200.
[0056] In addition, a first elastic element (not shown in the figure) is provided on the side wall of the excitation auxiliary chamber 221 along the Y direction. When the flexible excitation auxiliary component 400 is moved, the first elastic element is compressed by force to facilitate the adjustment of the flexible excitation auxiliary component 400. After the flexible excitation auxiliary component 400 is adjusted, the first elastic element is no longer subjected to external force and begins to rebound autonomously, thereby pressing against the flexible excitation auxiliary component 400 to prevent the flexible excitation auxiliary component 400 from shifting again after the position adjustment. A first guide groove is provided on each of the two side walls of the excitation auxiliary chamber 221 along the X direction. The two side walls of the flexible excitation auxiliary component 400 are slidably disposed in the two first guide grooves in a one-to-one correspondence to guide the flexible excitation auxiliary component 400 to slide along the Z direction.
[0057] Correspondingly, this embodiment provides three methods for adjusting the relative position of the imaging auxiliary hole 310 and the imaging through hole 230, as follows:
[0058] The first type involves an auxiliary imaging component 300 configured as a long strip corresponding to the imaging through-hole 230, which is detachably attached to the inner wall of the imaging through-hole 230. Each auxiliary imaging component 300 has an auxiliary imaging hole 310. Specifically, multiple auxiliary imaging components 300 are used, each with only one auxiliary imaging hole 310, and the position of the auxiliary imaging hole 310 on each component is different. When the specifications of the centrifuge tube 10 inserted into the reading box 200 change, simply remove the original auxiliary imaging component 300 and reattach the auxiliary imaging component 300 with the corresponding auxiliary imaging hole 310 to the inner wall of the imaging through-hole 230. For example, when the target centrifuge tube 10 is long, the auxiliary imaging component 300 with the auxiliary imaging hole 310 closer to the bottom wall of the reading box 200 is selected; when the target centrifuge tube 10 is short, the auxiliary imaging component 300 with the auxiliary imaging hole 310 closer to the top wall of the reading box 200 is selected.
[0059] The second type involves an auxiliary imaging component 300 configured as a long strip corresponding to the imaging through-hole 230, which is detachably attached to the inner wall of the imaging through-hole 230. Each auxiliary imaging component 300 has one such component, and multiple obstructable imaging auxiliary holes 310 are spaced apart along the Z-direction. When the specifications of the centrifuge tube 10 inserted into the reading box 200 change, simply obstruct the original imaging auxiliary hole 310 and then unobstruct the corresponding imaging auxiliary hole 310. For example, when the target centrifuge tube 10 is long, the corresponding imaging auxiliary hole near the bottom wall of the reading box 200 is unobstructed; when the target centrifuge tube 10 is short, the corresponding imaging auxiliary hole near the top wall of the reading box 200 is unobstructed.
[0060] The third type involves a photographing auxiliary chamber 231 extending along the Z-direction, provided in the reading box 200 corresponding to the photographing through-hole 230. The photographing auxiliary component 300 is a flexible plate and is movably installed within the photographing auxiliary chamber 231. The photographing auxiliary component 300 has a photographing auxiliary hole 310. Specifically, in conjunction with... Figure 2As shown, the side wall of the reading box 200, which has a shooting through hole 230, has a shooting auxiliary chamber 231 extending along the Z direction, corresponding to the shooting through hole 230, so that a sandwich is formed in the middle of the side wall. The flexible shooting auxiliary component 300 is movably disposed in the sandwich, and a shooting auxiliary hole 310 is provided in the middle of the flexible shooting auxiliary component 300. When the specifications of the centrifuge tube 10 inserted in the reading box 200 change, the flexible shooting auxiliary component 300 can be manually moved so that it moves along the Z direction in the shooting auxiliary chamber 231, thereby changing the relative position of the shooting auxiliary hole 310 and the shooting through hole 230. For example, when the target centrifuge tube 10 is long, the shooting auxiliary component 300 is moved towards the bottom wall of the reading box 200; when the target centrifuge tube 10 is short, the shooting auxiliary component 300 is moved towards the top wall of the reading box 200.
[0061] Additionally, a second elastic element (not shown in the figure) is provided on the side wall of the imaging auxiliary chamber 231 along the X direction. When the flexible imaging auxiliary component 300 is moved, the second elastic element is compressed to facilitate adjustment of the flexible imaging auxiliary component 300. After the flexible imaging auxiliary component 300 is adjusted, the second elastic element is no longer subjected to external force and begins to rebound autonomously, thereby pressing against the flexible imaging auxiliary component 300 to prevent it from shifting again after the position adjustment. Second guide grooves are provided on both side walls of the imaging auxiliary chamber 231 along the Y direction. The two side walls of the flexible imaging auxiliary component 300 are slidably disposed in the two second guide grooves to guide the flexible imaging auxiliary component 300 to slide along the Z direction.
[0062] Furthermore, the cross-sectional area of the excitation auxiliary hole 410 gradually increases from the excitation through hole 220 to the center of the readout box 200. With this configuration, as the cross-sectional area of the excitation auxiliary hole 410 gradually increases, the light emitted by the external excitation source gradually diffuses through the excitation through hole 220, which helps to increase the amount of light entering the readout box 200, thereby increasing the irradiation area of the external excitation source and ensuring that the fluorescence signal in the sample can be fully excited.
[0063] Furthermore, the fluorescence signal reading component also includes a cover plate 500, which is disposed on the outer wall of the reading box 200 corresponding to the placement through hole 210. In this embodiment, the cover plate 500 is configured as an opaque plate. After the centrifuge tube 10 containing the sample is inserted into the placement through hole 210, the cover plate 500 is disposed on the outer wall of the reading box 200. That is, the cover plate 500 blocks the open end of the centrifuge tube 10, preventing the sample in the centrifuge tube 10 from spilling out, while also preventing the excitation light from diffusing from the blocked open end of the centrifuge tube 10, thereby further ensuring that the fluorescence signal in the sample can be fully excited.
[0064] Preferably, the fluorescence signal reading component further includes a guide limiting component 600, and the cover plate 500 is movably disposed on the outer wall of the reading box 200 through the guide limiting component 600.
[0065] Specifically, in this embodiment, the guide limiting component 600 includes a limiting post 610 and a limiting groove 620. The limiting post 610 is an L-shaped component, with one end fixedly connected to the outer side of the top wall of the reading box 200 and located on one side of the storage through hole 210 along the X direction. The other end bends and extends towards the center of the storage through hole 210. The limiting groove 620 is disposed on the upper surface of the cover plate 500 corresponding to the limiting post 610. After the cover plate 500 is disposed on the outer wall of the reading box 200, the limiting post 610 is inserted into the limiting groove 620. Through the cooperation of the limiting post 610 and the limiting groove 620, the relative position of the cover plate 500 after being disposed on the reading box 200 is limited, preventing the cover plate 500 from shifting or falling off. The number of limiting posts 610 can be appropriately increased or decreased according to the actual situation, and the number of limiting grooves 620 is corresponding to the number of limiting posts 610. The position of the limit post 610 can also be adjusted according to the actual situation.
[0066] In some other embodiments, the guide limiting component 600 further includes a guide groove (not shown in the figure) and a guide rail (not shown in the figure). The guide groove is disposed on the outer side of the top wall of the reading box 200. Guide grooves are provided on both sides of the storage through hole 210 along the Y direction. Two guide rails are correspondingly provided on the lower end face of the cover plate 500. The two guide rails are slidably connected to the two guide grooves one-to-one. By slidingly engaging the guide rails with the corresponding guide grooves, the cover plate 500 is guided to slide along a predetermined path, thereby ensuring that the cover plate 500 can always completely cover the storage through hole 210 and prevent the excitation light from diffusing from the storage through hole 210.
[0067] Furthermore, a storage groove 240 is provided on the outer wall of the reading box 200. The storage groove 240 is concentrically arranged with the storage through hole 210 and cooperates with the storage through hole 210 to form a limiting step. This arrangement serves two purposes: First, the storage groove 240 provides sufficient clearance, preventing the open end of the centrifuge tube 10 from protruding beyond the top wall of the reading box 200 after it is inserted into the storage through hole 210. Therefore, when the cover plate 500 is fixedly connected to the outside of the top wall of the reading box 200, a gap is avoided between the cover plate 500 and the top wall of the reading box 200, further preventing the excitation light from diffusing from the storage through hole 210. Second, when the centrifuge tube 10 is inserted into the storage through hole 210, since the diameter of the open end of the centrifuge tube 10 is larger than the diameter of other parts, the open end of the centrifuge tube 10 can abut against the limiting step, preventing the centrifuge tube 10 from falling into the reading box 200.
[0068] An elastic limiting ring (not shown in the figure) is fixedly provided on the inner side of the storage through hole 210. When the centrifuge tube 10 is inserted into the storage through hole 210, the elastic limiting ring is opened under the action of external force to facilitate the insertion of the centrifuge tube 10. After the centrifuge tube 10 is inserted into the storage through hole 210, the elastic limiting ring rebounds on its own and abuts against the outer peripheral wall of the centrifuge tube 10, thereby further restricting the relative position of the centrifuge tube 10. This prevents the centrifuge tube 10 from falling into the reading box 200 and reduces the probability of displacement or shaking of the centrifuge tube 10 during the fluorescence signal imaging and reading process, thereby ensuring the reading effect and accuracy of the fluorescence signal.
[0069] The cross-sectional shapes of the imaging auxiliary hole 310, the excitation auxiliary hole 410, and the object placement through hole 210 can be set to rectangles, polygons, circles, ellipses, etc., depending on the actual situation. In this embodiment, the length of the reading box 200 is set to 90mm, the width to 50mm, and the height to 29mm; the imaging auxiliary hole 310 is set to a rectangular hole with a length of 10mm and a width of 10mm, and a second auxiliary hole is set on the side of the imaging auxiliary hole 310 near the inside of the reading box 200. The second auxiliary hole is set to a rectangular hole with a length of 6.5mm and a width of 6.5mm to facilitate the installation of filters 100 of different specifications in the imaging auxiliary hole 310 or the second auxiliary hole; the excitation auxiliary hole 410 is set to a circular hole, and the excitation auxiliary hole 410 is located opposite to the inside of the reading box 200. The diameter of one side of the reading box 200 is set to 7mm, and the diameter of the side closer to the reading box 200 is set to 9.6mm; the storage through hole 210 is a circular hole with a diameter of 6mm to facilitate the insertion of 200μL centrifuge tubes 10 into the reading box 200 through the storage hole; the depth of the storage groove 240 is set to 2mm; the cover plate 500 is a rectangular plate with a length of 19mm and a width of 15mm, and the limiting groove 620 on the cover plate 500 is a rectangular groove with a length of 3.5mm and a width of 2.5mm. The dimensions of the limiting post 610 are correspondingly set to the limiting groove 620. In other embodiments, the dimensions of the components in the fluorescence signal reading assembly and the specifications of the centrifuge tubes 10 are not limited to these and can be set according to the actual situation.
[0070] The fluorescence signal reading component provided in this embodiment can read not only the fluorescence signal of the liquid sample in the centrifuge tube 10, but also the color signal or phosphorescence signal, etc., of the liquid sample in the centrifuge tube 10. In addition to the centrifuge tube 10, the sample carrier for the liquid sample can also be a test tube, a container bottle, a glass slide 20, or a test paper 30, etc. That is, in some other embodiments, the fluorescence signal reading component can also read the fluorescence signal, luminescence signal, or color signal on the liquid sample carrier such as a test tube, container bottle, glass slide 20, or test paper 30.
[0071] Test tubes or containers can be directly inserted into the storage through hole 210 and extended into the reading box 200, which will not be described in detail here. The slide 20 and test paper 30 require the assistance of the auxiliary reading plate 250 and auxiliary clip 260.
[0072] Combination Figure 4 and Figure 5 As shown, the principle of signal reading for slide 20 and test paper 30 is the same. In this embodiment, the signal reading for slide 20 is taken as an example. Specifically, the reading box 200 also includes an auxiliary reading plate 250 and an auxiliary clip 260. The lower end face of the auxiliary reading plate 250 is provided with a hook 251. The auxiliary clip 260 can be detachably hung on the hook 251 to increase the cross-sectional area of the storage through hole 210. When it is necessary to read fluorescence, luminescence, or color signals from a glass slide 20 containing a liquid sample, the glass slide 20 containing the liquid sample is first clamped in the auxiliary clip 260, and then the auxiliary clip 260 is hooked to the hook 251. Next, the auxiliary reading plate 250 is placed in the storage groove 240. In this state, the auxiliary clip 260 extends through the storage through-hole 210 into the reading box 200, and the glass slide 20 is located inside the reading box 200. Then, the fluorescence, luminescence, or color signals of the liquid sample on the glass slide 20 are read through the excitation auxiliary hole 410 and the imaging auxiliary hole 310. Besides being hooked to the auxiliary reading plate 250 via the hook 251, the auxiliary clip 260 can also be set on the lower end face of the auxiliary reading plate 250 by welding, bonding, snap-fitting, threaded connection, interference fit, or integral molding. Alternatively, the auxiliary clip 260 can be directly set on the lower end face of the cover plate 500. Alternatively, the slide 20 can be placed inside the reading box 200 using other auxiliary components, such as a suspension wire.
[0073] In addition, the fluorescence signal reading component provided in this embodiment can also read fluorescence signals, luminescence signals, or color signals of solid or gas samples. Specifically, to read the target signal of a solid sample, the solid sample to be read can be clamped in the auxiliary clamp 260 so that the solid sample is located inside the reading box 200, thereby realizing the reading of the target signal of the solid sample; when reading the target signal of a gas sample, the gas sample to be read is first placed in a sample carrier with a transparent sealed cavity, such as a bottle with a sealed cap; then the sample carrier with a transparent sealed cavity is inserted into the placement through hole 210, or clamped in the auxiliary clamp 260 so that the sample carrier with a transparent sealed cavity is located inside the reading box 200, thereby realizing the reading of the target signal of the gas sample. Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. For those skilled in the art, various obvious changes, readjustments, and substitutions can be made without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model shall be included within the scope of protection of the claims of this utility model.
Claims
1. A fluorescence signal reading component for reading the fluorescence signal of a sample in a centrifuge tube (10), characterized in that, include: Filter (100); A reading box (200) has a side wall with a spaced-out through hole (210), an excitation through hole (220), and a shooting through hole (230). The center lines of the spaced-out through hole (210), the excitation through hole (220), and the shooting through hole (230) are perpendicular to each other and intersect at the same point. The filter (100) is detachably mounted on the reading box (200) corresponding to the shooting through hole (230). The centrifuge tube (10) is placed in the reading box (200) through the placement through hole (210). When the laser source irradiates the centrifuge tube (10) through the excitation through hole (220), the camera can capture and read the fluorescence signal of the sample in the centrifuge tube (10) through the imaging through hole (230).
2. The fluorescence signal reading component according to claim 1, characterized in that, The shooting through hole (230) is configured as an elongated hole extending along the Z direction. The reading box (200) is adjustablely provided with a shooting auxiliary component (300). The shooting auxiliary component (300) is provided with a shooting auxiliary hole (310) corresponding to the shooting through hole (230). The excitation through hole (220) is configured as an elongated hole extending along the Z direction. The reading box (200) is adjustablely provided with an excitation auxiliary component (400). The excitation auxiliary component (400) is provided with an excitation auxiliary hole (410) corresponding to the excitation through hole (220).
3. The fluorescence signal reading component according to claim 2, characterized in that, The shooting auxiliary component (300) is configured as a long strip corresponding to the shooting through hole (230) and can be detachably abutted against the inner wall of the shooting through hole (230); The shooting aid (300) is provided with a shooting aid hole (310), or, along the Z direction, the shooting aid (300) is provided with a plurality of obstructable shooting aid holes (310) at intervals.
4. The fluorescence signal reading component according to claim 2, characterized in that, The reading box (200) is provided with a shooting auxiliary chamber (231) extending in the Z direction corresponding to the shooting through hole (230). The shooting auxiliary component (300) is a flexible plate and is movably installed in the shooting auxiliary chamber (231). The shooting auxiliary component (300) is provided with a shooting auxiliary hole (310).
5. The fluorescence signal reading component according to claim 2, characterized in that, The excitation auxiliary component (400) is configured as a long strip corresponding to the excitation through hole (220) and can be detachably abutted against the inner wall of the excitation through hole (220); The excitation aid (400) is provided with one excitation aid hole (410), or, along the Z direction, the excitation aid (400) is provided with a plurality of shieldable excitation aid holes (410) at intervals.
6. The fluorescence signal reading component according to claim 2, characterized in that, The reading box (200) is provided with an excitation auxiliary chamber (221) extending in the Z direction corresponding to the excitation through hole (220). The excitation auxiliary component (400) is a flexible plate and is movably installed in the excitation auxiliary chamber (221). The excitation auxiliary component (400) is provided with an excitation auxiliary hole (410).
7. The fluorescence signal reading component according to claim 2, characterized in that, The cross-sectional area of the excitation auxiliary hole (410) gradually increases from the excitation through hole (220) to the center of the reading box (200).
8. The fluorescence signal reading component according to any one of claims 1-7, characterized in that, The fluorescence signal reading component also includes a cover plate (500), which is disposed on the outer wall of the reading box (200) corresponding to the placement through hole (210).
9. The fluorescence signal reading component according to claim 8, characterized in that, The fluorescence signal reading component further includes a guide limiting component (600), and the cover plate (500) is movably disposed on the outer wall of the reading box (200) through the guide limiting component (600).
10. The fluorescence signal reading component according to claim 8, characterized in that, The outer wall of the reading box (200) is also provided with a storage groove (240), the storage groove (240) and the storage through hole (210) are concentrically arranged, and cooperate with the storage through hole (210) to form a limiting step; And / or, an elastic limiting ring is fixedly provided on the inner side of the storage through hole (210).