Polypeptide cracking and depositing tank

Abstract

The utility model relates to the technical field of depositing tanks, in particular to a polypeptide cracking depositing tank. In order to mainly solve the problems that the purity of a target peptide chain is reduced, the subsequent purification is difficult, the cost is increased and the production quality requirement is difficult to meet due to the fact that upper-layer suspended matters are easy to carry when materials are discharged through a bottom discharge pipe after resin precipitation in an existing precipitation tank, the following technical scheme is provided: the precipitation tank comprises a tank body, and the outer side of the tank body is sleeved with a cooling sleeve; the stirring assembly is mounted on the tank body and is used for stirring the resin in the tank body; the floating liquid removing mechanism is arranged on the tank body and the stirring assembly and is used for sucking suspension liquid after resin precipitation in the tank body; and the feeding pipe is mounted at the top of the tank body. The device can improve the purity of a target peptide chain, reduce the subsequent purification difficulty and cost, ensure the quality stability of a polypeptide product, meet strict production quality standards and improve the polypeptide synthesis production efficiency and economic benefits.

Description

Technical Field

[0001] This utility model relates to the field of sedimentation tank technology, and in particular to a polypeptide lysis sedimentation tank. Background Technology

[0002] Polypeptides are bioactive substances widely involved in various cellular functions within organisms. Their molecular structure lies between that of amino acids and proteins, composed of multiple amino acids linked by peptide bonds in a specific sequence. In the polypeptide synthesis process, the hydroxyl groups of the terminal amino acids of the target peptide chain are first covalently linked to an insoluble polymer resin. The amino acids on the solid support serve as the amino group. After removing the protecting amino groups, they react with an excess of activated hydroxyl groups to link the long peptide chain. The target peptide chain length is achieved through repeated cycles of condensation, washing, deprotection, neutralization and washing, and subsequent condensation. The cleavage of the peptide chain from the resin is a crucial step in the synthesis process. The resin precipitation stage is particularly important and must be completed in a precipitation tank, directly affecting the purity and efficiency of subsequent peptide chain separation.

[0003] Currently, existing precipitation tanks typically discharge the precipitated portion through a drain pipe at the bottom after resin precipitation. However, this traditional discharge method has significant drawbacks. When discharging the bottom precipitate, especially near the end, a large amount of upper suspended matter is inevitably carried over. This contamination not only reduces the purity of the target peptide chain, significantly increasing the difficulty and cost of subsequent purification processes, but also affects the quality stability of the final peptide product, making it difficult to meet increasingly stringent production and quality standards. Therefore, this invention proposes a peptide lysis precipitation tank. Utility Model Content

[0004] The purpose of this invention is to address the problem in the prior art that existing sedimentation tanks, when discharging material through the bottom discharge pipe after resin precipitation, easily carry over upper suspended matter, leading to reduced purity of the target peptide chain, difficulty in subsequent purification, increased costs, and difficulty in meeting production quality requirements. This invention proposes a peptide lysis sedimentation tank.

[0005] The technical solution of this utility model is as follows: a polypeptide lysis precipitation tank, including a tank body, with a cooling sleeve sleeved on the outside of the tank body; a stirring assembly installed on the tank body, the stirring assembly being used to stir the resin in the tank body; a deflocculation mechanism provided on the tank body and the stirring assembly, the deflocculation mechanism being used to remove the suspension after resin precipitation in the tank body; a feed pipe installed on the top of the tank body, and a spray pipe connected to the top of the tank body, with a spray ball installed at the bottom of the spray pipe.

[0006] Optionally, the stirring assembly includes a rotating sleeve rotatably connected to the top of the tank, a drive rod slidably connected in the rotating sleeve, the drive rod being a regular square prism, a stirring rod fixedly connected to the bottom of the drive rod, multiple sets of stirring blades installed on the outer periphery of the stirring rod, a positioning ring provided below the stirring rod, the stirring rod being slidably connected in the positioning ring, and multiple sets of fixing rods fixedly connected between the positioning ring and the tank.

[0007] Optionally, the stirring assembly further includes a mounting frame fixedly connected to the top of the tank. The mounting frame is U-shaped. A servo motor is mounted on the top of the mounting frame. The output end of the servo motor passes through the mounting frame and is fixedly connected to a first gear. A second gear is provided on one side of the first gear and meshes with it. The second gear is fixedly connected to the outer ring of the rotating sleeve.

[0008] Optionally, the deflocculation mechanism includes a positioning sleeve fixedly connected to the top of the drive rod, a first moving plate rotatably connected to the outer ring of the positioning sleeve, and two sets of first push rod motors installed on the top of the tank, the output ends of the two sets of first push rod motors being fixedly connected to the first moving plate.

[0009] Optionally, the deflocculation mechanism further includes a connecting rod fixedly connected to the outer ring of the stirring rod. The connecting rod is located below multiple sets of stirring blades. The driving rod, stirring rod, and connecting rod all share a drainage channel. The bottom of the connecting rod has multiple through holes communicating with the drainage channel. A connecting sleeve is installed on the top of the driving rod. A connecting pipe is installed above the connecting sleeve. A rubber ring is installed at the bottom of the connecting pipe. A micro pump is connected to the top of the connecting pipe via a pipeline. The output end of the micro pump is connected to a sewage pipe.

[0010] Optionally, the deflocculation mechanism further includes a second movable plate fixedly connected to the outer ring of the connecting pipe. Two sets of second push rod motors are installed on the top of the tank. The output ends of the two sets of second push rod motors pass through the second movable plate and are fixedly connected to a limiting plate. A spring is sleeved on the output end of the second push rod motor, and the spring is located between the second movable plate and the limiting plate. A support ring is sleeved on the output end of the second push rod motor, and the support ring is located below the second movable plate.

[0011] Optionally, a discharge pipe is connected to the bottom of the tank, and a solenoid valve is installed on the tank.

[0012] Optionally, the cooling sleeve is connected to an inlet pipe and an outlet pipe on both sides, with the inlet pipe located above the outlet pipe.

[0013] Optionally, an eyepiece is installed on the top of the tank.

[0014] Optionally, a control box is also included, wherein the solenoid valve, servo motor, first push rod motor, micro pump, and second push rod motor are all electrically connected to the control box.

[0015] In summary, this application includes at least one of the following beneficial technical effects:

[0016] This invention, through the setting of a deflocculation mechanism, after sedimentation, uses a first push rod motor and a second push rod motor to adjust the height of the connecting rod and seal the connection. A micro pump is then used to completely suck out the suspension above the sediment. Compared with traditional sedimentation tanks, this design fundamentally avoids the mixing of suspended matter into the sediment during discharge, which greatly improves the purity of the final discharged sediment, reduces the difficulty and cost of subsequent purification processes, and ensures the quality stability of peptide products.

[0017] The sedimentation process is further accelerated by driving the stirring components with a servo motor, the discharge is precisely controlled by a solenoid valve, and the operation of the deflocculation mechanism is automatically adjusted by a push rod motor. Operators can observe the internal situation through a visor and clean the equipment by spraying it with a spray pipe without much manual intervention. This not only improves the ease of operation but also enhances the stability and production efficiency of the equipment.

[0018] In summary, this invention can improve the purity of the target peptide chain, reduce the difficulty and cost of subsequent purification, ensure the quality stability of peptide products, meet strict production quality standards, and improve the efficiency and economic benefits of peptide synthesis. Attached Figure Description

[0019] Figure 1 A schematic diagram of a peptide lysis precipitation tank is provided.

[0020] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0021] Figure 3 for Figure 1 A schematic diagram of the cross-sectional structure;

[0022] Figure 4 This is a schematic diagram of the cross-sectional structure of the connecting pipe.

[0023] Figure label:

[0024] 1. Tank body; 11. Discharge pipe; 12. Solenoid valve;

[0025] 2. Cooling sleeve; 21. Inlet pipe; 22. Outlet pipe;

[0026] 3. Stirring assembly; 31. Rotating sleeve; 32. Drive rod; 33. Stirring rod; 34. Stirring blade; 35. Positioning ring; 36. Fixing rod; 37. Mounting frame; 38. Servo motor; 39. First gear; 310. Second gear;

[0027] 4. Deflocculation mechanism; 41. Positioning sleeve; 42. First moving plate; 43. First push rod motor; 44. Connecting rod; 45. Drainage channel; 46. Through hole; 47. Connecting sleeve; 48. Connecting pipe; 49. Micro pump; 410. Second moving plate; 411. Second push rod motor; 412. Limiting plate; 413. Spring; 414. Support ring;

[0028] 5. Feed pipe; 6. Spray pipe; 61. Spray ball;

[0029] 7. Eyepiece. Detailed Implementation

[0030] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some embodiments of this utility model, but not all embodiments.

[0031] The components of the present invention embodiments described and shown in the accompanying drawings can typically be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0032] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0033] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0034] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0035] Example

[0036] like Figure 1 and Figure 3 As shown, this utility model proposes a peptide lysis precipitation tank, including a tank body 1. A discharge pipe 11 is connected to the bottom of the tank body 1 to facilitate the discharge of precipitates and efficiently transport the precipitated resin and other materials to subsequent processing stages. A solenoid valve 12 is installed on the tank body 1 to control the opening and closing of the discharge pipe 11. It can be flexibly switched on and off according to the precipitation progress and discharge requirements, precisely controlling material discharge. A cooling sleeve 2 is fitted around the outside of the tank body 1. An inlet pipe 21 and an outlet pipe 22 are connected to both sides of the cooling sleeve 2, respectively. The inlet pipe 21 is located above the outlet pipe 22. A circulating refrigeration device is connected through the inlet pipe 21 and the outlet pipe 22 to cool the tank body 1, ensuring that the peptide lysis and precipitation process inside the tank body 1 takes place at a suitable temperature, effectively preventing temperature changes from affecting the material reaction and precipitation effect.

[0037] For further details, please refer to Figure 2 and Figure 3The aforementioned sedimentation tank includes a stirring assembly 3 mounted on the tank body 1. The stirring assembly 3 is used to stir the resin in the tank body 1, accelerating resin dispersion and mixing, and significantly improving sedimentation efficiency. The stirring assembly 3 includes a rotating sleeve 31 rotatably connected to the top of the tank body 1. The rotating sleeve 31 rotates in its original position, providing a stable power transmission basis for the stirring action. A drive rod 32 is slidably connected within the rotating sleeve 31. The drive rod 32 is a regular square prism, allowing the rotating sleeve 31 to drive the drive rod 32 to rotate synchronously without hindering the sliding of the drive rod 32 within the rotating sleeve 31. A stirring rod 33 is fixedly connected to the bottom of the drive rod 32. Multiple sets of stirring blades 34 are mounted on the outer periphery of the stirring rod 33. When the drive rod 32 rotates, it drives the stirring rod 33 and the multiple sets of stirring blades 34 to rotate synchronously, stirring the resin in the tank body 1 and promoting sedimentation. Through multi-angle and multi-directional stirring actions, the resin fully contacts the reaction environment, accelerating the sedimentation process. A positioning ring 35 is provided below the stirring rod 33, and the stirring rod 33 is slidably connected to the positioning ring 35. The positioning ring 35 ensures the stability of the stirring rod 33 during the stirring process and avoids uneven stirring or damage to components due to shaking. Multiple sets of fixing rods 36 are fixedly connected between the positioning ring 35 and the tank body 1, so that the position of the positioning ring 35 is fixed and further enhances the structural stability of the stirring assembly 3. The stirring assembly 3 also includes a mounting frame 37 fixedly connected to the top of the tank body 1. The mounting frame 37 is U-shaped and its fixed position provides reliable mounting support for the servo motor 38. The servo motor 38 is mounted on the top of the mounting frame 37. The output end of the servo motor 38 passes through the mounting frame 37 and is fixedly connected to the first gear 39. After the servo motor 38 is started, it drives the first gear 39 to rotate, providing a stable and adjustable stirring power source. A second gear 310 is provided on one side of the first gear 39 and meshes with it. The second gear 310 is fixedly connected to the outer ring of the rotating sleeve 31. When the first gear 39 rotates, it drives the second gear 310 to rotate synchronously, thereby driving the rotating sleeve 31 to rotate. Through the gear transmission structure, the efficient transmission and stable output of power are achieved.

[0038] Furthermore, such as Figures 1 to 4As shown, the sedimentation tank also includes a deflocculation mechanism 4 mounted on the tank body 1 and the stirring assembly 3. The deflocculation mechanism 4 is used to absorb the suspension after resin sedimentation in the tank body 1, effectively removing the upper suspended matter and preventing the discharge from carrying suspended matter, thus significantly improving the purity of the sediment. The deflocculation mechanism 4 includes a positioning sleeve 41 fixedly connected to the top of the drive rod 32. A first moving plate 42 is rotatably connected to the outer ring of the positioning sleeve 41. Under the action of the positioning sleeve 41, the first moving plate 42 drives the drive rod 32 to move synchronously when it rises and falls, without hindering the rotation of the drive rod 32. This facilitates adjustment of the height of the connecting rod 44, allowing for thorough absorption of the suspension at the top of the sediment. The absorption position can be precisely adjusted according to the actual sedimentation situation. Two sets of first push rod motors 43 are installed on the top of the tank body 1. The output ends of both sets of first push rod motors 43 are fixedly connected to the first moving plate 42. The first push rod motors 43 are used to drive the first moving plate 42 to rise and fall, making operation convenient and efficient. The descaling mechanism 4 also includes a connecting rod 44 fixedly connected to the outer ring of the stirring rod 33. The connecting rod 44 is located below multiple sets of stirring blades 34. When the stirring rod 33 rotates, it drives the connecting rod 44 to rotate synchronously, so as to effectively absorb the suspension during the rotation process. The drive rod 32, stirring rod 33 and connecting rod 44 are all provided with a drainage channel 45. The bottom of the connecting rod 44 is provided with multiple sets of through holes 46 communicating with the drainage channel 45, so as to draw out the upper part of the suspension of sediment in the tank 1 through the drainage channel 45 and the through holes 46, forming an efficient suspension absorption channel. A connecting sleeve 47 is installed on the top of the drive rod 32. A connecting pipe 48 is provided above the connecting sleeve 47. A rubber ring is installed at the bottom of the connecting pipe 48. After the connecting pipe 48 is pressed into the connecting sleeve 47, the rubber ring achieves a seal, ensuring no leakage during the suspension absorption process and ensuring the absorption effect. A micro pump 49 is connected to the top of the connecting pipe 48 via a pipeline. The output end of the micro pump 49 is connected to a drain pipe. The micro pump 49 generates suction to draw out the suspension in the tank 1 and collect it through the drain pipe for easy processing, thus realizing the automated collection and treatment of the suspension. The desuspension mechanism 4 also includes a second movable plate 410 fixedly connected to the outer ring of the connecting pipe 48. The second movable plate 410 moves synchronously with the connecting pipe 48. Two sets of second push rod motors 411 are installed on the top of the tank body 1. The output ends of the two sets of second push rod motors 411 pass through the second moving plate 410 and are fixedly connected to the limiting plate 412. A spring 413 is sleeved on the output end of the second push rod motor 411. The spring 413 is located between the second moving plate 410 and the limiting plate 412. When the second push rod motor 411 shortens, it drives the second moving plate 410 to move downward. At the same time, after the connecting pipe 48 contacts the connecting sleeve 47, the limiting plate 412 continues to move downward and squeezes the spring 413 to prevent hard squeezing. At the same time, the elastic force of the spring 413 ensures that the connecting sleeve 47 and the connecting pipe 48 are in tight contact to prevent leakage. This facilitates the suction of the suspension after the micro pump 49 is started, ensuring the reliability and sealing of the connection structure.A support ring 414 is fitted onto the output end of the second push rod motor 411. The support ring 414 is located below the second moving plate 410. When the second push rod motor 411 extends, it drives the second moving plate 410 to move upward through the support ring 414, thereby realizing convenient opening and closing control of the connecting pipe 48 and the connecting sleeve 47.

[0039] Specifically, the aforementioned sedimentation tank includes a feed pipe 5 installed at the top of the tank body 1, facilitating the addition of materials to the tank body 1 and enabling rapid and convenient material delivery. A spray pipe 6 is also connected to the top of the tank body 1, with spray balls 61 installed at the bottom of the spray pipe 6. This allows for cleaning of the inside of the tank body 1 or the addition of cleaning solutions, reaction solutions, etc., ensuring the cleanliness of the tank body 1 and the full reaction of the materials. An eyepiece 7 is installed at the top of the tank body 1 for easy observation of the sedimentation process inside, allowing operators to monitor the sedimentation progress in real time and adjust equipment operating parameters accordingly.

[0040] Furthermore, the aforementioned sedimentation tank also includes a control box. The solenoid valve 12, servo motor 38, first push rod motor 43, micro pump 49, and second push rod motor 411 are all electrically connected to the control box, realizing automated and coordinated control of various components of the equipment, improving the convenience of operation and the stability of equipment operation.

[0041] In this embodiment, the polypeptide lysis mixture to be precipitated is injected into the tank 1 through the feed pipe 5. The circulating refrigeration equipment is started, and the coolant enters the cooling sleeve 2 through the liquid inlet pipe 21 to cool the tank 1 and optimize the precipitation environment.

[0042] The control box starts the servo motor 38, which drives the rotating sleeve 31 to rotate through the meshing of the first gear 39 and the second gear 310. The rotating sleeve 31 drives the stirring rod 33 and the stirring blade 34 to rotate through the square prism-shaped drive rod 32, accelerating the mixing reaction of the resin and the solution and promoting precipitation. The positioning ring 35 ensures the stability of the stirring process.

[0043] After sedimentation, the first pusher motor 43 drives the first moving plate 42 to rise, which in turn moves the drive rod 32 and connecting rod 44 down to the suspension layer above the sediment via the positioning sleeve 41. The second pusher motor 411 shortens, pushing the second moving plate 410 to press the connecting pipe 48 into the connecting sleeve 47, with the spring 413 ensuring a seal. The micro pump 49 starts, sucking out the upper suspension through the drain channel 45 and through hole 46, and discharging it through the drain pipe. The stirring rod 33 drives the connecting rod 44 to rotate, ensuring that the suction area covers the entire cross-section and that the suspension is completely removed.

[0044] After the desquamation mechanism 4 completes its operation and resets, the control box opens the solenoid valve 12, and the sediment is discharged through the discharge pipe 11. Because the upper suspension has been removed beforehand, the purity of the finally discharged sediment is significantly improved. The inner wall of the tank 1 can be cleaned through the spray pipe 6 and spray balls 61. Operators can observe the internal status in real time through the eyepiece 7 to ensure the normal operation of each process.

[0045] The above specific embodiments are merely optional embodiments of this utility model. Based on the technical solution of this utility model and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A polypeptide lysis precipitation tank, characterized in that, Comprising: A tank body (1), with a cooling sleeve (2) sleeved outside the tank body (1); A stirring component (3) installed on the tank body (1), and the stirring component (3) is used for stirring the resin in the tank body (1); A floating liquid removing mechanism (4) arranged on the tank body (1) and the stirring component (3), and the floating liquid removing mechanism (4) is used for sucking the suspension after the resin in the tank body (1) precipitates; A feed pipe (5) installed on the top of the tank body (1), a spray pipe (6) is also connected to the top of the tank body (1), and a spray ball (61) is installed at the bottom of the spray pipe (6).

2. The polypeptide lysis precipitation tank according to claim 1, characterized in that, The stirring component (3) includes a rotating sleeve (31) rotatably connected to the top of the tank body (1), a driving rod (32) is slidably connected in the rotating sleeve (31), the driving rod (32) is arranged in a regular quadrangular prism shape, a stirring rod (33) is fixedly connected to the bottom of the driving rod (32), multiple groups of stirring blades (34) are installed on the outer circumference of the stirring rod (33), a positioning ring (35) is arranged below the stirring rod (33), the stirring rod (33) is slidably connected in the positioning ring (35), and multiple groups of fixing rods (36) are fixedly connected between the positioning ring (35) and the tank body (1).

3. The polypeptide lysis precipitation tank according to claim 2, characterized in that, The stirring component (3) further includes a mounting frame (37) fixedly connected to the top of the tank body (1), the mounting frame (37) is arranged in a "冂" shape, a servo motor (38) is installed on the top of the mounting frame (37), the output end of the servo motor (38) penetrates through the mounting frame (37) and is fixedly connected with a first gear (39), a second gear (310) meshing with the first gear (39) is arranged on one side of the first gear (39), and the second gear (310) is fixedly connected to the outer ring of the rotating sleeve (31).

4. The polypeptide lysis precipitation tank according to claim 3, characterized in that, The floating liquid removing mechanism (4) includes a positioning sleeve (41) fixedly connected to the top of the driving rod (32), a first moving plate (42) is rotatably connected to the outer ring of the positioning sleeve (41), two groups of first push rod motors (43) are installed on the top of the tank body (1), and the output ends of the two groups of first push rod motors (43) are fixedly connected with the first moving plate (42).

5. The polypeptide lysis precipitation tank according to claim 4, characterized in that, The floating liquid removing mechanism (4) further includes a connecting rod (44) fixedly connected to the outer circle of the stirring rod (33), the connecting rod (44) is arranged below multiple groups of stirring blades (34), a liquid discharge channel (45) is jointly opened in the driving rod (32), the stirring rod (33) and the connecting rod (44), multiple through holes (46) communicating with the liquid discharge channel (45) are opened at the bottom of the connecting rod (44), a connecting sleeve (47) is installed on the top of the driving rod (32), a connecting pipe (48) is arranged above the connecting sleeve (47), a rubber ring is installed at the bottom of the connecting pipe (48), the top of the connecting pipe (48) is connected to a micro pump (49) through a pipeline, and the output end of the micro pump (49) is connected to a sewage discharge pipe.

6. The polypeptide lysis precipitation tank according to claim 5, characterized in that, The deflocculation mechanism (4) further includes a second movable plate (410) fixedly connected to the outer ring of the connecting pipe (48). Two sets of second push rod motors (411) are installed on the top of the tank (1). The output ends of the two sets of second push rod motors (411) pass through the second movable plate (410) and are fixedly connected to the limiting plate (412). A spring (413) is sleeved on the output end of the second push rod motor (411). The spring (413) is located between the second movable plate (410) and the limiting plate (412). A support ring (414) is sleeved on the output end of the second push rod motor (411). The support ring (414) is located below the second movable plate (410).

7. A polypeptide lysis precipitation tank according to claim 6, characterized in that, The bottom of the tank (1) is connected to a discharge pipe (11), and a solenoid valve (12) is installed on the tank (1).

8. The polypeptide lysis precipitation tank according to claim 7, characterized in that, The cooling sleeve (2) is connected to an inlet pipe (21) and an outlet pipe (22) on both sides respectively, with the inlet pipe (21) located above the outlet pipe (22).

9. A polypeptide lysis precipitation tank according to claim 8, characterized in that, An eyepiece (7) is installed on the top of the tank (1).

10. A polypeptide lysis precipitation tank according to claim 7, characterized in that, It also includes a control box, and the solenoid valve (12), servo motor (38), first push rod motor (43), micro pump (49) and second push rod motor (411) are all electrically connected to the control box.

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