Double-system reaction tube
By designing a dual-system reaction tube, solution A and solution B can be mixed without opening the cap, solving the environmental pollution and detection error problems caused by opening the test tube in the existing technology, and improving detection efficiency and accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- 重庆市南岸区疾病预防控制中心
- Filing Date
- 2025-05-30
- Publication Date
- 2026-05-12
AI Technical Summary
In nucleic acid amplification detection, existing test tubes require opening the cap when adding the second solution, which exposes the reacted solution to air, easily causing environmental pollution and gene leakage, affecting the accuracy of the test results. Furthermore, the detection process is cumbersome and costly, making it unsuitable for rapid detection.
Design a dual-system reaction tube that uses two side-by-side test tubes and a specific cap structure to achieve mixing of solution A and solution B without opening the cap. Seal is achieved using components such as baffles, sealing plugs, and rubber rings to prevent the solutions from contacting air.
It effectively avoids contact between the solution and air, reduces environmental pollution and detection errors, simplifies the operation steps, and improves detection efficiency.
Smart Images
Figure CN224227054U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of test tube technology, specifically to a dual-system reaction tube. Background Technology
[0002] In medical testing, disease control centers, and routine laboratories, test tubes are crucial tools for sample collection, reaction, and storage. Disease control centers typically use test tubes for virus detection, bacterial culture, immunoassay, and molecular biology testing. Currently, the structure of test tubes in the testing field remains largely unchanged, usually consisting of a cylindrical tube with a spherical bottom. Chinese patent document CN209061162U discloses a test tube comprising: a tube body with a receiving space inside and an opening at the top, and a first magnetic suction component on the tube body; and a cap, located at the top of the tube body, covering the opening, and equipped with a second magnetic suction component adapted to the first magnetic suction component. The cap is fixed to the tube body by the magnetic attraction between the second and first magnetic suction components. This test tube effectively solves the problem of inconvenience in existing technologies where the cap is connected to the tube body via threads. However, nucleic acid amplification detection involves two sequential reactions: a solution (e.g., solution A) reacts with the added sample for a period of time, followed by the addition of another solution (e.g., solution B) to react with the solution after the first reaction for calibration, thus obtaining the test result. During the process of adding solution B after the reaction of solution A, opening the test tube cap can easily expose the reacted solution A to air. Especially during nucleic acid amplification, the amplification products are highly susceptible to leakage due to opening the cap, causing environmental gene contamination and potential infection of operators by airborne aerosols. This affects the accuracy of the test results and causes environmental pollution (especially for the detection of infectious bacteria, potentially leading to the spread of infectious diseases). Currently, the common practice for this test is to cool the test tube containing the reacted solution A in a 4°C refrigerator under low pressure and sterile conditions before adding solution B. However, this method is cumbersome, costly, inefficient, and carries the risk of gene fragment leakage, making it unsuitable for single-use, rapid testing. Utility Model Content
[0003] To address the problems existing in the prior art, the purpose of this utility model is to provide a dual-system reaction tube that allows solution A to react first, and then solution A to be mixed with solution B without opening the cap, effectively avoiding environmental and reagent contamination caused by opening the cap.
[0004] The objective of this utility model is achieved through the following technical solution:
[0005] A dual-system reaction tube includes a test tube body and a cap. The test tube body consists of two test tubes arranged side by side, with their sidewalls connected. The cap is provided corresponding to the test tube body and includes a cap plate and a sealing plug. The sealing plug is fixedly disposed on the bottom surface of the cap plate, and the outer ring of the sealing plug matches the inner ring of the test tube body. Multiple sealing rubber rings are evenly arranged along the axial direction of the outer ring of the sealing plug. The size of the cap plate is larger than the size of the outer wall of the test tube body.
[0006] Based on further optimization of the above scheme, a baffle is slidably installed at the connection point of the two test tubes to block and seal the connection area of the two test tubes, so as to prevent the solutions in the two test tubes from mixing first and causing detection errors.
[0007] Based on further optimization of the above scheme, rubber layers are respectively provided on the bottom surface and side wall of the baffle that are in contact with the test tube body to achieve sealing and prevent leakage.
[0008] Based on further optimization of the above scheme, a positioning head is set on the top of the baffle through a support column. The longitudinal section of the positioning head is a conical structure with a smaller top and a larger bottom. A through hole is opened in the middle of the sealing plug and a positioning sleeve is slidably set in the through hole. A groove is opened in the middle of the bottom surface of the positioning sleeve corresponding to the positioning head, and multiple sets of clip assemblies are set on the inner wall of the groove corresponding to the positioning head. The connection between the cover and the baffle is realized through the connection between the positioning sleeve and the positioning head.
[0009] Based on further optimization of the above scheme, the clip assembly includes a slider and a return spring. Multiple spring grooves are evenly opened on the side wall of the groove and around the central axis of the positioning sleeve, and the slider is slidably arranged in the spring groove. The slider is connected to the bottom of the corresponding spring groove through a return spring. The bottom surface of the slider away from the return spring is provided with an inclined surface structure that matches the positioning head.
[0010] Based on further optimization of the above scheme, the diameter of the positioning sleeve is greater than the width of the baffle, and the diameter of the groove is less than the width of the baffle.
[0011] Based on further optimization of the above scheme, the top surface of the positioning sleeve is connected to the bottom surface of the cover plate through a tension spring; at least two sliding rods are provided on the top surface of the positioning sleeve and on the outer ring of the tension spring, with the end of the sliding rod away from the positioning sleeve penetrating the cover plate; a fixing groove is opened on the top surface of the cover plate and a limiting block is provided at the top of the sliding rod corresponding to the fixing groove; a positioning shaft is provided on the top surface of the fixing groove and between the limiting blocks; a turntable is rotatably provided on the outer wall of the upper side of the fixing groove, and a blind hole is opened on the turntable corresponding to the limiting block.
[0012] Based on further optimization of the above scheme, the outer diameter of the limiting block is larger than the diameter of the slide rod to prevent the top of the slide rod from disengaging from the fixing groove; the outer diameter of the turntable is larger than the diameter of the fixing groove, thereby achieving the closure of the fixing groove.
[0013] The following are the technical effects of this utility model:
[0014] The reaction tube provided in this application uses two side-by-side test tubes to add solution A and solution B respectively. A cap consisting of a cover plate and a sealing plug seals the test tube body, thus preventing solution A from coming into contact with air during and after the reaction, which could lead to environmental or reagent contamination. After solution A has reacted, solution A and solution B are mixed by pouring the test tube body without opening the cap, further preventing reagent contamination from contact with the atmosphere. Furthermore, this reaction tube eliminates the need for repeated opening and closing of the cap and the step-by-step addition of solutions A and B, effectively simplifying the detection process and improving detection efficiency. Attached Figure Description
[0015] Figure 1 This is a schematic diagram of the reaction tube in an embodiment of the present invention.
[0016] Figure 2 for Figure 1 A magnified view of part A in the image.
[0017] Figure 3 for Figure 1 BB-direction sectional view.
[0018] Figure 4 This is a diagram showing the usage state of the reaction tube in an embodiment of this utility model.
[0019] Figure 5 This is a schematic diagram of another embodiment of the reaction tube of this utility model.
[0020] Among them, 100 is solution A; 200 is solution B; 10 is the test tube body; 11 is the handle; 21 is the cover plate; 211 is the positioning shaft; 212 is the turntable; 22 is the sealing plug; 221 is the sealing rubber ring; 30 is the baffle; 31 is the support column; 32 is the positioning head; 40 is the positioning sleeve; 411 is the slider; 412 is the return spring; 42 is the tension spring; 431 is the slide rod; and 432 is the limit block. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.
[0022] Example 1:
[0023] A dual-system reaction tube includes a test tube body 10 and a cap. The test tube body 10 consists of two test tubes arranged side by side, with the side walls of the two test tubes 10 connected to each other (in combination). Figure 1 and Figure 3 As shown; at the same time, as Figure 3As shown, the cross-section of the test tube body 10 is an "∞" structure with the central intersection points connected. The cover is provided corresponding to the test tube body 10, including a cover plate 21 and a sealing plug 22. The sealing plug 22 is fixedly mounted on the bottom surface of the cover plate 21, and the outer ring of the sealing plug 22 matches the inner ring of the test tube body 10. Multiple sealing rubber rings 221 are evenly arranged along the axial direction of the outer ring of the sealing plug 22 (the sealing rubber rings 221 achieve a tight fit between the outer wall of the sealing plug 22 and the inner wall of the test tube body 10, thereby sealing the opening of the test tube body 10, as shown). Figure 1 As shown), the size of the cover plate 21 is larger than the outer wall size of the test tube body 10 (e.g. Figure 1 As shown, the outer ring of the cover plate 21 protrudes from the outer wall of the test tube body 10 in all directions.
[0024] A baffle 30 is slidably installed at the connection point of the two test tubes (e.g.) Figure 1 or Figure 3 As shown), the baffle 30 is used to shield and seal the connection area between the two test tubes, preventing the solutions in the two test tubes from mixing first and causing detection errors. Rubber layers (using conventional rubber materials in this field) are provided on the bottom surface and side walls of the baffle 30 where it contacts the test tube body 10 to achieve a seal and prevent leakage. A positioning head 32 is provided at the top of the baffle 30 via a support column 31 (the support column 31 and the positioning head 32 are coaxial, and the central axis of the support column 31 is collinear with the vertical centerline of the baffle 30). The longitudinal cross-section of the positioning head 32 is a tapered structure with a smaller top and a larger bottom (e.g., ...). Figure 2 (As shown); a through hole is opened in the middle of the sealing plug 22, and a positioning sleeve 40 is slidably arranged in the through hole. A groove is opened in the middle of the bottom surface of the positioning sleeve 40 corresponding to the positioning head 32, and multiple sets of clamping assemblies are arranged in the inner wall of the groove corresponding to the positioning head 32 (the clamping assemblies are generally 2 to 4 sets, and 4 sets are used in this application). The clamping assembly includes a slider 411 and a return spring 412. Multiple spring grooves are evenly opened on the side wall of the groove around the central axis of the positioning sleeve 40 (in this embodiment, since there are four sets of clamping assemblies, there are 4 spring grooves). The slider 411 is slidably arranged in the spring groove. The slider 411 is connected to the bottom of the corresponding spring groove through the return spring 412. The bottom surface of the end of the slider 411 away from the return spring 412 is provided with an inclined structure that matches the positioning head 32 (e.g. Figure 2 As shown, the bottom surface of the end of the slider 411 away from the corresponding return spring 412 is an inclined surface structure that slopes from the central axis of the groove towards the side wall; the diameter of the positioning sleeve 40 is larger than the width of the baffle 30, and the diameter of the groove is smaller than the width of the baffle 30 (e.g., Figure 2 (As shown). The connection between the cover and the baffle 30 is achieved through the connection between the positioning sleeve 40 and the positioning head 32.
[0025] The top surface of the positioning sleeve 40 is connected to the bottom surface of the cover plate 21 via a tension spring 42 (in combination). Figure 1 and Figure 2As shown); at least two slide rods 431 are provided on the top surface of the positioning sleeve 40 and on the outer ring of the tension spring 42 (in this embodiment, two slide rods 431 are used and the two slide rods 431 are arranged symmetrically). The end of the slide rod 431 away from the positioning sleeve 40 passes through the cover plate 21; a fixing groove is opened on the top surface of the cover plate 21 and a limiting block 432 is provided on the top surface of the fixing groove corresponding to the fixing groove. A positioning shaft 211 is provided on the top surface of the fixing groove and between the limiting blocks 432. A turntable 212 is rotatably provided on the outer wall of the upper side of the fixing groove. A blind hole is opened on the turntable 212 corresponding to the limiting block 432 (under the initial condition, the limiting block 432 is located inside the fixing groove and is pushed against the non-blind hole position of the turntable 212, and the tension spring 42 is in the extended state). The outer diameter of the limiting block 432 is larger than the diameter of the slide rod 431 to prevent the top of the slide rod 431 from leaving the fixing groove; the outer diameter of the turntable 212 is larger than the diameter of the fixing groove, thereby realizing the closure of the fixing groove (as shown). Figure 1 (As shown).
[0026] Working principle:
[0027] In use, the test tube body 10 is placed vertically. Due to gravity, the baffle 30 slides down, causing the bottom surface of the baffle 30 to adhere to the top surface of the inner cavity of the connection area of the test tube body 10, thereby dividing the test tube body 10 into two regions (e.g., Figure 1 (As shown); then, add solution A 100 and solution B 200 to the left and right areas of the test tube body 10 respectively, and quickly put the cap on the opening of the test tube body 10; when the cap is put on, the sealing rubber ring 221 of the outer ring of the sealing plug 22 fits against the inner cavity of the test tube body 10 to form a seal. At the same time, the positioning sleeve 40 moves down with the cap, which does not require center positioning, due to the specific "∞" structure of the test tube body 10, so that the positioning head 32 cooperates with the groove; when the slider 411 contacts the side of the positioning head 32, due to the action of the positioning head 32 The force causes the slider 411 to slide in the corresponding spring groove away from the positioning head 32. The return spring 412 is compressed, and the positioning sleeve 40 continues to move downward. The top surface of the slider 411 is located below the bottom surface of the positioning head 32. The slider 411 is pushed out by the elastic force of the return spring 412 and then locked onto the lower side of the positioning head 32, realizing the connection between the positioning sleeve 40 and the baffle 30. (During this process, due to the continuous downward movement of the positioning sleeve 40, the baffle 30 is squeezed downward, further ensuring the tightness of the connection between the baffle 30 and the test tube body 10.) Next, place the test tube body 10 with the cap installed under specific conditions (such as heating or water cooling, depending on the specific test item) for 20-30 minutes to allow solution A 100 to react preferentially; then rotate the turntable 212 so that the blind hole of the turntable 212 corresponds to the limiting block 432. At this time, due to the elastic force of the tension spring 42, the positioning sleeve 40 and the baffle 30 are pulled upward a certain distance, thereby opening the bottom of the connection area between the two test tubes (such as...). Figure 4(as shown in the figure). Finally, tilt the test tube body 10 so that the reacted solution A 100 mixes with solution B 200 through the opening at the bottom of the baffle 30. The entire process does not require opening the cap, effectively avoiding environmental or reagent contamination.
[0028] Example 2:
[0029] As a further optimization of the present invention, based on the solution of Embodiment 1, for ease of operation, as follows: Figure 5 As shown, a handle 11 is provided on one side of the outer wall of the test tube body 10 to facilitate the handling of the test tube body 10; at the same time, in order to ensure the tightness of the installation between the test tube body 10 and the cap, a positioning clip is provided on the outer wall of the test tube body 10 above the handle 11, and the cap 21 is located on the bottom surface of the outer ring of the test tube body 10 and is provided with an elastic clip corresponding to the positioning clip. The connection between the cap and the test tube body 10 is achieved by fixing the positioning clip and the elastic clip (e.g., Figure 5 (As shown).
[0030] Example 3:
[0031] As a further optimization of the present utility model, based on the solution of embodiment 1, in order to avoid the turntable 212 being accidentally rotated during operation, causing the positioning sleeve 40 to move upward prematurely and fail to connect with the baffle 30, a through hole is provided on one side of the outer ring of the blind hole on the turntable 212, and a positioning groove is opened on the top surface of the cover plate 21 corresponding to the through hole. The rotation of the turntable 212 is hard-limited by the positioning pin passing through the through hole from top to bottom and connecting with the positioning groove. When rotation is required, the positioning pin can be pulled out.
Claims
1. A dual-system reaction tube, characterized in that: The test tube body consists of two test tubes arranged side by side with their sidewalls connected. The cap is provided corresponding to the test tube body and includes a cap plate and a sealing plug. The sealing plug is fixedly installed on the bottom surface of the cap plate and its outer ring matches the inner ring of the test tube body. Multiple sealing rubber rings are evenly arranged along the axial direction of the outer ring of the sealing plug. The size of the cap plate is larger than the size of the outer wall of the test tube body.
2. The dual-system reaction tube according to claim 1, characterized in that: A baffle is slidably installed at the connection point between the two test tubes.
3. A dual-system reaction tube according to claim 2, characterized in that: The bottom surface and side walls of the baffle that contact the test tube body are respectively provided with rubber layers.
4. A dual-system reaction tube according to claim 2 or 3, characterized in that: The baffle is topped with a positioning head via a support column. The longitudinal section of the positioning head is a tapered structure with a smaller top and a larger bottom. A through hole is opened in the middle of the sealing plug, and a positioning sleeve is slidably installed inside the through hole. A groove is opened in the middle of the bottom surface of the positioning sleeve corresponding to the positioning head, and multiple sets of clip assemblies are installed on the inner wall of the groove corresponding to the positioning head.
5. A dual-system reaction tube according to claim 4, characterized in that: The clip assembly includes a slider and a return spring. Multiple spring grooves are evenly opened on the side wall of the groove and around the central axis of the positioning sleeve. The slider is slidably arranged in the spring groove. The slider is connected to the bottom of the corresponding spring groove through a return spring. The bottom surface of the slider away from the return spring is provided with an inclined surface structure that matches the positioning head.
6. A dual-system reaction tube according to claim 4, characterized in that: The diameter of the positioning sleeve is greater than the width of the baffle, and the diameter of the groove is less than the width of the baffle.
7. A dual-system reaction tube according to claim 4, characterized in that: The top surface of the positioning sleeve is connected to the bottom surface of the cover plate via a tension spring; at least two sliding rods are provided on the top surface of the positioning sleeve and on the outer ring of the tension spring, with the end of the sliding rod away from the positioning sleeve penetrating the cover plate; a fixing groove is opened on the top surface of the cover plate and a limiting block is provided at the top of the sliding rod corresponding to the fixing groove; a positioning shaft is provided on the top surface of the fixing groove and between the limiting blocks; a turntable is rotatably provided on the outer wall of the upper side of the fixing groove, and a blind hole is opened on the turntable corresponding to the limiting block.
8. A dual-system reaction tube according to claim 7, characterized in that: The outer diameter of the limiting block is larger than the diameter of the sliding rod; the outer diameter of the turntable is larger than the diameter of the fixing groove.