Bacterial liquid spraying device for manufacturing mouse skin bacterial infection model

Abstract

The utility model provides a bacterial liquid spraying device for manufacturing a mouse skin bacterial infection model, which comprises a fixed cylinder and two support rods, a through groove is arranged at the top of the fixed cylinder, a mounting groove is arranged at the front end of the through groove at the top of the fixed cylinder, a fixing mechanism is arranged in the fixed cylinder, sliding grooves are arranged at the top of the fixed cylinder on both sides of the through groove, and the sliding grooves are communicated with the through groove. Mounting plates are fixedly connected to the two ends of the top of the fixed cylinder, a motor is fixedly mounted on one side of the mounting plate at one end, a moving mechanism is arranged between the two mounting plates, and limiting mechanisms are arranged on the portions, on the two sides of the moving mechanism, between the two mounting plates. And then a moving block is driven to move, so that the moving block drives an adjusting plate below to move from the tail of the nude mouse to the head of the nude mouse, and uniform spraying is performed on the skin of the nude mouse through a through groove by utilizing a bacterial liquid spray head, so that the accuracy and repeatability of a research result are better, and the biological safety is better guaranteed.

Description

Technical Field

[0001] This utility model mainly relates to the field of biomedical technology, specifically to a bacterial solution spraying device for creating a mouse skin bacterial infection model. Background Technology

[0002] As a model animal, nude mice can be used to create different types of skin infection models by applying different kinds of bacteria to their skin. Then, candidate drugs can be used to evaluate the therapeutic effect on the model, which is applied to the non-clinical evaluation of drugs.

[0003] Currently, there are two methods for applying bacterial suspension to the skin of nude mice: one is to manually hold the mouse, use a pipette to draw up the bacterial suspension, and directly apply it to the mouse's skin, then use a pipette tip to spread the suspension evenly; the other is to manually hold the mouse and use a sterile cotton swab to apply the bacterial suspension along the midline of the mouse's back for inoculation. The disadvantages of these two methods are as follows: First, manual application is not easy to achieve even distribution, easily leading to significant differences in the amount of bacterial suspension applied between individuals; second, the applied bacterial suspension does not dry easily, and mice returning to their cages may easily transfer the suspension to other mice or the cage walls, resulting in poor accuracy and reproducibility of the research results; third, when applying the suspension to each mouse, there is a greater chance of the bacterial suspension being exposed to air, which can easily cause biosafety issues. Summary of the Invention

[0004] This invention mainly provides a bacterial solution spraying device for creating a bacterial infection model of mouse skin, in order to solve the technical problems mentioned in the background art.

[0005] The technical solution adopted by this utility model to solve the above-mentioned technical problems is as follows:

[0006] A bacterial solution spraying device for creating a mouse skin bacterial infection model includes a fixed cylinder and two support rods. The top of the fixed cylinder has a through groove, and the top of the fixed cylinder has an installation groove at the front end of the through groove. A fixing mechanism is provided inside the fixed cylinder.

[0007] The top of the fixed cylinder has sliding grooves on both sides of the through groove. The top of the fixed cylinder is fixedly connected to both ends of the fixed cylinder. A motor is fixedly installed on one side of one end of the mounting plate. A moving mechanism is provided between the two mounting plates, and a limiting mechanism is provided on both sides of the moving mechanism between the two mounting plates.

[0008] Both of the support rods are fixedly connected to the front side with a fixing rod, and the two fixing rods are connected to an adjustment plate through a rotating shaft. The top of the adjustment plate is provided with a spraying assembly.

[0009] Preferably, the fixing mechanism includes a first fixing block, a second fixing block, and a locking nut. The first fixing block is slidably fitted inside one end of the fixing cylinder, and the second fixing block is installed inside the fixing cylinder at the end away from the first fixing block. The tops of the first fixing block and the second fixing block are both threaded with locking nuts.

[0010] Preferably, the moving mechanism includes a threaded rod and a moving block. The threaded rod is connected to the two mounting plates by a rotating shaft, and one end of the threaded rod is connected to the output shaft of the motor. The moving block is threaded to the outer side of one end of the threaded rod.

[0011] Preferably, a connecting plate is fixedly connected to the bottom of the movable block, and an electric push rod is fixedly installed at the bottom of the connecting plate, and the extension end of the electric push rod is fixedly connected to one end of the bottom of the adjusting plate;

[0012] Preferably, the limiting mechanism includes a limiting rod and a limiting block. The two mounting plates are fixedly connected to the limiting rod on both sides of the moving mechanism. The limiting rod is movably connected to the limiting block through a mounting hole on its outer side. The two limiting blocks are connected to the moving block through a connecting block at one end. The two limiting blocks are fixedly connected to the support rod at the end away from the moving block.

[0013] Preferably, both the support rod and the fixed rod are fixedly connected to rollers at their bottom ends, and the rollers are disposed inside the groove.

[0014] Preferably, the spraying assembly includes a bacterial liquid reservoir, a first pipe, a bacterial liquid nozzle, a baffle, a warm air connection port, a second pipe, and a warm air nozzle. A bacterial liquid reservoir is fixedly installed at one end of the top of the adjusting plate, and one end of the bacterial liquid reservoir is connected to the first pipe. A bacterial liquid nozzle is fixedly installed at one end of the first pipe, and a baffle is fixedly installed on the outside of the bacterial liquid nozzle.

[0015] A warm air connection port is fixedly installed on the bottom of the adjustment plate on the side away from the electric push rod, and a second pipe is fixedly connected to one side of the warm air connection port, and a warm air nozzle is connected to one end of the second pipe.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] 1. The motor drives the threaded rod to rotate through its output shaft, which in turn moves the moving block. This causes the moving block to move the adjusting plate below from the tail of the nude mouse towards the head, and then the bacterial solution is evenly sprayed onto the skin of the nude mouse through the permeable trough using a spray nozzle. This improves the accuracy and repeatability of the research results and better ensures biosafety.

[0018] 2. The warm air nozzle can be used to dry the mouse skin after spraying the bacterial solution, ensuring that the solution adheres better to the skin surface and avoids sticking to other mice or cage walls, thus improving the accuracy and reproducibility of the research results.

[0019] 3. The bacterial solution spray nozzle is equipped with a baffle on the outside, and both the bacterial solution spray nozzle and the warm air spray nozzle spray the bacterial solution and dry the nude mice through a transparent groove. The relatively sealed environment can reduce the probability of the bacterial solution being exposed to the air, better prevent biosafety risks, and protect the health of operators.

[0020] 4. The electric push rod extension drives one end of the adjustment plate upward, thereby adjusting the tilt angle of the adjustment plate, and thus adjusting the spraying angle of the spraying component, so as to better colonize the bacterial solution into the hair follicles of the experimental mouse skin.

[0021] The present invention will be explained in detail below with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0023] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0024] Figure 3 This is a schematic diagram of the internal structure of the fixed cylinder of this utility model;

[0025] Figure 4 This is a top view of the fixed cylinder structure of this utility model;

[0026] Figure 5 This is a rear view of the top structure of the fixed cylinder of this utility model;

[0027] Figure 6 This is a schematic diagram of the structure of the first fixing block and the locking nut of this utility model;

[0028] Figure 7 This is a schematic diagram of the bacterial liquid spray nozzle and baffle structure of this utility model;

[0029] Figure 8 This is a schematic diagram of the connection structure between the second pipe and the warm air nozzle of this utility model.

[0030] The attached figures are labeled as follows: 1. Fixed cylinder; 2. Support rod; 3. Through groove; 4. Mounting groove; 5. Fixing mechanism; 501. First fixing block; 502. Second fixing block; 503. Locking nut; 6. Slide groove; 7. Mounting plate; 8. Motor; 9. Moving mechanism; 901. Threaded rod; 902. Moving block; 10. Limiting mechanism; 1001. Limiting rod; 1002. Limiting block; 11. Fixing rod; 12. Adjusting plate; 13. Spraying assembly; 1301. Bacterial liquid storage container; 1302. First pipe; 1303. Bacterial liquid nozzle; 1304. Baffle; 1305. Warm air connection port; 1306. Second pipe; 1307. Warm air nozzle; 14. Connecting plate; 15. Electric push rod; 16. Roller. Detailed Implementation

[0031] To facilitate understanding of this utility model, a more comprehensive description of the utility model will be given below with reference to the accompanying drawings, which show several embodiments of the utility model. However, the utility model can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the disclosure of the utility model more thorough and comprehensive.

[0032] Example

[0033] Please refer to the appendix carefully. Figure 1-8A bacterial solution spraying device for creating a mouse skin bacterial infection model includes a fixed cylinder 1 and two support rods 2. The top of the fixed cylinder 1 has a through groove 3 for spraying the bacterial solution. At the front end of the through groove 3, the top of the fixed cylinder 1 also has an installation groove 4 for installing and fixing related components. The installation groove 4 begins at the front end of the through groove 3 on the top of the fixed cylinder 1. The fixed cylinder 1 has a fixing mechanism 5 inside, including a first fixing block 501, a second fixing block 502, and a locking nut 503. The first fixing block 501 is slidably mounted on one end of the fixed cylinder 1, and the second fixing block 502 is installed at the end of the fixed cylinder 1 away from the first fixing block 501. The tops of both the first fixing block 501 and the second fixing block 502 are threaded together. Locking nuts 503 are provided, and the locking nuts 503 on the top of the first fixing block 501 are set in the through groove 3, while the locking nuts 503 on the top of the second fixing block 502 are set in the mounting groove 4, to ensure the stability and reliability of the fixing mechanism 5. Sliding grooves 6 are provided on both sides of the through groove 3 at the top of the fixing cylinder 1. Mounting plates 7 are fixedly connected to both ends of the top of the fixing cylinder 1. A motor 8 is fixedly installed on one side of one mounting plate 7. A moving mechanism 9 is provided between the two mounting plates 7. The moving mechanism 9 includes a threaded rod 901 and a moving block 902. The threaded rod 901 is connected between the two mounting plates 7 via a rotating shaft, and one end of it is connected to the output shaft end of the motor 8. The moving block 902 is threadedly connected to the outer side of the threaded rod 901. Driven by the motor 8, the moving mechanism 901 moves... Block 902 can move on threaded rod 901, thereby realizing the movement function of the device. To ensure the stability and accuracy of the movement process, two limiting mechanisms 10 are respectively provided on both sides of the moving mechanism 9 between the two mounting plates 7. The limiting mechanism 10 includes a limiting rod 1001 and a limiting block 1002. The limiting rod 1001 is fixedly connected between the two mounting plates 7, and one end of its outer side is movably connected to the limiting block 1002 through a mounting hole. One end of the two limiting blocks 1002 is connected to the moving block 902 through a connecting block, and the other end is fixedly connected to the support rod 2. The front side of the support rod 2 is fixedly connected to a fixing rod 11, and the bottom end of the support rod 2 and the fixing rod 11 are fixedly connected to rollers 16. The rollers 16 are set inside the slide groove 6, and the rollers 16 are arranged inside the slide groove 6. The rolling of the roller 16 within the chute 6 ensures smooth movement of the device. An adjusting plate 12 is connected between the two fixed rods 11 via a rotating shaft. A spraying assembly 13 is mounted on the top of the adjusting plate 12 to complete the spraying operation of the bacterial solution. The spraying assembly 13 includes a bacterial solution reservoir 1301, a first pipe 1302, a bacterial solution nozzle 1303, a baffle 1304, a warm air connection port 1305, a second pipe 1306, and a warm air nozzle 1307. A bacterial solution reservoir 1301 is fixedly installed at one end of the top of the adjusting plate 12 for storing the bacterial solution. One end of the bacterial solution reservoir 1301 is connected to the first pipe 1302, and a bacterial solution nozzle 1303 is fixedly installed at one end of the first pipe 1302 for evenly spraying the bacterial solution onto the mouse skin.To prevent external interference during the spraying process, a baffle 1304 is fixedly installed on the outside of the bacterial spray nozzle 1303. A warm air connection port 1305 is fixedly installed on the bottom of the adjusting plate 12 on the side away from the electric push rod 15. A second pipe 1306 is fixedly connected to one side of the warm air connection port 1305. One end of the second pipe 1306 is connected to a warm air nozzle 1307, and the other end of the warm air connection port 1305 is connected to a warm air system through a corrugated pipe to facilitate the delivery of warm air to the warm air nozzle. Through the warm air nozzle 1307, the bacterial solution can be applied to the mouse skin after spraying. A drying process is performed to ensure that the bacterial solution adheres better to the skin surface and avoids sticking to other mice or cage walls, thus improving the accuracy and reproducibility of research results. A connecting plate 14 is fixedly connected to the bottom of the moving block 902, and an electric push rod 15 is fixedly installed at the bottom of the connecting plate 14. The extended end of the electric push rod 15 is fixedly connected to one end of the bottom of the adjusting plate 12. Driving the electric push rod 15 causes one end of the adjusting plate 12 to move upward, thereby adjusting the tilt angle of the adjusting plate 12, and consequently adjusting the spraying angle of the spraying assembly 13, facilitating better colonization of the bacterial solution into the hair follicles of the experimental mouse skin.

[0034] The specific operation method of this utility model is as follows:

[0035] In use, the user can place the nude mouse inside the fixing tube 1, and then fix the first fixing block 501 and the second fixing block 502 by tightening the locking nut 503, thereby fixing the nude mouse and preventing it from moving around. After fixing, the drive motor 8 drives the threaded rod 901 to rotate at its output shaft end, which in turn drives the moving block 902 to move. This causes the moving block 902 to move the lower adjustment plate 12 from the tail of the nude mouse towards the head, and then start spraying the nude mouse skin with the bacterial solution spray nozzle 1303 through the permeable groove 3, thereby reducing the difference in bacterial solution application between individuals. The baffle 1304 on the outside of the bacterial solution spray nozzle 1303 can reduce the probability of bacterial solution being exposed to the air, better preventing biosafety risks. At the same time as spraying, the warm air nozzle 1307 blows out warm air to make the bacterial solution dry quickly and avoid sticking to other mice or cage walls, making the research results more accurate and reproducible.

[0036] The extension end of the electric push rod 15 can drive one end of the adjustment plate 12 to rise, thereby adjusting the angle of the spraying component 13, so as to better colonize the bacterial solution into the hair follicles of the experimental mouse skin.

[0037] The present invention has been described above by way of example in conjunction with the accompanying drawings. Obviously, the specific implementation of the present invention is not limited to the above-described manner. Any non-substantial improvement made by adopting the inventive concept and technical solution of the present invention, or the direct application of the inventive concept and technical solution of the present invention to other occasions without modification, shall be within the protection scope of the present invention.

Claims

1. A bacterial spraying device for making a mouse skin bacterial infection model, comprising a fixed cylinder (1) and two support rods (2), wherein the top of the fixed cylinder (1) is provided with a through groove (3), and the top of the fixed cylinder (1) is provided with an installation groove (4) at the front end of the through groove (3), and a fixing mechanism (5) is provided inside the fixed cylinder (1). The top of the fixed cylinder (1) has sliding grooves (6) on both sides of the through groove (3). The top two ends of the fixed cylinder (1) are fixedly connected to mounting plates (7). A motor (8) is fixedly installed on one side of the mounting plate (7). A moving mechanism (9) is provided between the two mounting plates (7), and a limiting mechanism (10) is provided on both sides of the moving mechanism (9) between the two mounting plates (7). The two support rods (2) are fixedly connected to the front side of the two support rods (2), and the two fixed rods (11) are connected to the adjustment plate (12) through a rotating shaft. The top of the adjustment plate (12) is provided with a spraying assembly (13).

2. The bacterial solution spraying device for creating a mouse skin bacterial infection model according to claim 1, characterized in that, The fixing mechanism (5) includes a first fixing block (501), a second fixing block (502), and a locking nut (503). The first fixing block (501) is slidably assembled at one end of the fixing cylinder (1), and the second fixing block (502) is installed at the end of the fixing cylinder (1) away from the first fixing block (501). The tops of the first fixing block (501) and the second fixing block (502) are both threaded with locking nuts (503).

3. The bacterial solution spraying device for creating a mouse skin bacterial infection model according to claim 1, characterized in that, The moving mechanism (9) includes a threaded rod (901) and a moving block (902). The threaded rod (901) is connected to the shaft between the two mounting plates (7). One end of the threaded rod (901) is connected to the output shaft end of the motor (8), and the moving block (902) is threaded to the outside of one end of the threaded rod (901).

4. The bacterial solution spraying device for creating a mouse skin bacterial infection model according to claim 3, characterized in that, The bottom of the movable block (902) is fixedly connected to a connecting plate (14), and an electric push rod (15) is fixedly installed at the bottom of the connecting plate (14), and the extended end of the electric push rod (15) is fixedly connected to one end of the bottom of the adjusting plate (12).

5. The bacterial solution spraying device for creating a mouse skin bacterial infection model according to claim 1, characterized in that, The limiting mechanism (10) includes a limiting rod (1001) and a limiting block (1002). The two mounting plates (7) are fixedly connected to the limiting rod (1001) on both sides of the moving mechanism (9). The limiting rod (1001) is movably connected to the limiting block (1002) through a mounting hole on one side. The two limiting blocks (1002) are connected to the moving block (902) through a connecting block at one end. The two limiting blocks (1002) are fixedly connected to the support rod (2) at the end away from the moving block (902).

6. The bacterial solution spraying device for creating a mouse skin bacterial infection model according to claim 1, characterized in that, Both the support rod (2) and the fixing rod (11) are fixedly connected to rollers (16) at their bottom ends, and the rollers (16) are set inside the groove (6).

7. The bacterial solution spraying device for creating a mouse skin bacterial infection model according to claim 1, characterized in that, The spraying assembly (13) includes a bacterial liquid reservoir (1301), a first pipe (1302), a bacterial liquid nozzle (1303), a baffle (1304), a hot air connection port (1305), a second pipe (1306), and a hot air nozzle (1307). The bacterial liquid reservoir (1301) is fixedly installed at one end of the top of the adjusting plate (12), and one end of the bacterial liquid reservoir (1301) is connected to the first pipe (1302). The bacterial liquid nozzle (1303) is fixedly installed at one end of the first pipe (1302), and a baffle (1304) is fixedly installed on the outside of the bacterial liquid nozzle (1303). The bottom of the adjustment plate (12) is fixedly installed with a warm air connection port (1305) on the side away from the electric push rod (15), and a second pipe (1306) is fixedly connected to one side of the warm air connection port (1305), and a warm air nozzle (1307) is connected to one end of the second pipe (1306).

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