High-throughput cell culture and real-time monitoring device based on micro-fluidic chip

Abstract

The utility model belongs to the technical field of microfluidics, and particularly discloses a high-flux cell culture and real-time monitoring device based on a micro-fluidic chip, which comprises a mounting folded plate, the micro-fluidic chip, two storage containers and a monitoring sensing assembly, the micro-fluidic chip is arranged on the upper surface of a transverse plate of the mounting folded plate, two supply-in micro-channels and an output micro-channel are arranged in the micro-fluidic chip, the tail ends of the two supply-in micro-channels intersect to form a culture bin, and one end of the output micro-channel is communicated with the culture bin; the two storage containers are arranged on the vertical plate of the mounting folded plate, and the bottom ends of the two storage containers are connected with the micro-flow output pipe. The micro-fluidic chip, the storage container, the micro-fluidic control pump, the monitoring sensing component and the like are integrated on the mounting folded plate to form a stable whole, so that the cell culture process in the micro-fluidic chip is convenient to observe during use.

Description

Technical Field

[0001] This utility model relates to the field of microfluidics technology, specifically a high-throughput cell culture and real-time monitoring device based on a microfluidic chip. Background Technology

[0002] Microfluidic chip technology integrates the basic operational units of biological, chemical, and medical analysis processes, such as sample preparation, reaction, separation, and detection, onto a single chip at the micrometer scale, automating the entire analysis process.

[0003] Microfluidic chips have a wide range of applications, such as monitoring the growth status of cells cultured within them. However, current methods for monitoring cell culture typically involve combining multiple devices, which is fragmented and difficult to operate. Utility Model Content

[0004] The purpose of this invention is to provide a high-throughput cell culture and real-time monitoring device based on microfluidic chips to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a high-throughput cell culture and real-time monitoring device based on a microfluidic chip, comprising:

[0006] Install the folding plate, microfluidic chip, two storage containers, and monitoring sensor components;

[0007] The mounting plate includes a horizontal plate and a vertical plate disposed on one side of the horizontal plate. The microfluidic chip is disposed on the upper surface of the horizontal plate of the mounting plate. The microfluidic chip is provided with an inlet microchannel and an outlet microchannel. There are two inlet microchannels. The ends of the two inlet microchannels intersect to form a culture chamber. One end of the outlet microchannel is connected to the culture chamber.

[0008] Two storage containers are mounted on the upright plate of the mounting plate. The bottom of each storage container is connected to a microfluidic output tube, and a microfluidic control pump is installed on each microfluidic output tube. The other end of each microfluidic output tube is connected to two supply microchannels. A mounting bracket is provided on the upright plate of the mounting plate, and a monitoring sensor component is installed on the mounting bracket.

[0009] Preferably, a support groove is provided in the middle of the horizontal plate of the mounting plate, a transparent support plate is embedded in the support groove, the microfluidic chip is located on the upper surface of the transparent support plate, and a supplementary lighting device corresponding to the position of the transparent support plate is provided on the lower surface of the mounting plate.

[0010] Preferably, the supplementary lighting device includes a support plate connected to the lower surface of the mounting plate by a support column, and lighting lamp beads are arranged at equal intervals on the upper surface of the support plate.

[0011] Preferably, support strips are provided at both ends of the lower surface of the mounting plate, and the bottom end of the support strip is lower than the lower surface of the bearing plate.

[0012] Preferably, the monitoring sensing component includes a fluorescence sensor and an image acquisition camera.

[0013] Preferably, a drainage collection box is provided on the upper surface of the horizontal plate of the mounting folding plate, and an output pipe is connected to the output microchannel, the output pipe being connected to the drainage collection box.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] The microfluidic chip, storage container, microfluidic control pump, monitoring and sensing components are integrated on the mounting plate to form a stable whole, which facilitates the observation of the cell culture process inside the microfluidic chip during use. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a structural diagram of the mounting plate, support groove, transparent support plate, and bearing plate of this utility model.

[0018] Figure 3 This is a schematic diagram of the internal structure of the microfluidic chip of this utility model.

[0019] In the diagram: 1. Mounting plate; 2. Support groove; 3. Transparent support plate; 4. Bearing plate; 5. Illumination lamp beads; 6. Support strip; 7. Microfluidic chip; 8. Inlet microchannel; 9. Outlet microchannel; 10. Outlet tube; 11. Drainage collection box; 12. Mounting bracket; 13. Monitoring and sensing components; 14. Storage container; 15. Microfluidic output tube; 16. Microfluidic control pump. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0022] Example 1:

[0023] Please see Figure 1-3 This utility model provides a technical solution: a high-throughput cell culture and real-time monitoring device based on a microfluidic chip, comprising: a mounting plate 1, a microfluidic chip 7, two storage containers 14 and a monitoring and sensing component 13;

[0024] The mounting plate 1 includes a horizontal plate and a vertical plate disposed on one side of the horizontal plate. The microfluidic chip 7 is disposed on the upper surface of the horizontal plate of the mounting plate 1. The microfluidic chip 7 is provided with an inlet microchannel 8 and an outlet microchannel 9. There are two inlet microchannels 8, and the ends of the two inlet microchannels 8 intersect to form a culture chamber. One end of the outlet microchannel 9 is connected to the culture chamber. Two storage containers 14 are disposed on the vertical plate of the mounting plate 1. The bottom ends of the two storage containers 14 are connected to microfluidic output tubes 15. Microfluidic control pumps 16 are installed on the microfluidic output tubes 15. The other ends of the two microfluidic output tubes 15 are respectively connected to the two inlet microchannels 8. A mounting bracket 12 is disposed on the vertical plate of the mounting plate 1, and a monitoring sensor component 13 is installed on the mounting bracket 12.

[0025] Analysis of the above content: Before use, the cells to be cultured and the liquid culture medium required for the cells are stored in two separate storage containers 14. During culture, the cells and liquid culture medium are intermittently aspirated by the microfluidic pump 16. The cells and liquid culture medium are respectively introduced into the microfluidic chip 7 through the supply microchannel 8. In the culture chamber, the cells and liquid culture medium come into contact and are cultured. After the culture is completed, the cells and liquid culture medium are continued to be supplied. Under the push of air pressure, the cultured cells and liquid culture medium in the culture chamber are pushed out and output through the output microchannel 9.

[0026] During the cultivation process, the cultivation status is monitored by the monitoring sensor component 13, and the data monitored by the monitoring sensor component 13 is uploaded to the host computer. The host computer can output control to the microfluidic control pump 16 (the microfluidic control pump 16, the monitoring sensor component 13, the host computer, etc. all use the corresponding equipment in the existing microfluidics, which will not be described in detail here).

[0027] The temperature and other conditions required for cell culture can be supplied externally, depending on the specific circumstances.

[0028] Example 2:

[0029] Please see Figure 1-3 This utility model provides a technical solution based on Embodiment 1: A support groove 2 is provided in the middle of the horizontal plate of the mounting folding plate 1, and a transparent support plate 3 is embedded in the support groove 2. The microfluidic chip 7 is located on the upper surface of the transparent support plate 3, and a supplementary lighting device corresponding to the position of the transparent support plate 3 is provided on the lower surface of the mounting folding plate 1. The supplementary lighting device includes a support plate 4 connected to the lower surface of the mounting folding plate 1 by a support column, and lighting lamp beads 5 are arranged at equal intervals on the upper surface of the support plate 4. Support bars 6 are provided at both ends of the lower surface of the mounting folding plate 1, and the bottom end of the support bar 6 is lower than the lower surface of the support plate 4.

[0030] Analysis of the above content: The lighting bead 5 is powered by an external power source. After the lighting bead 5 is lit, the light passes through the transparent support plate 3 to supplement the microfluidic chip 7, which facilitates the monitoring sensor component 13 to collect the corresponding data. If some data collection does not require the light to be turned on, the power supply circuit of the lighting bead 5 can be cut off.

[0031] Example 3:

[0032] Please see Figure 1-3 Based on Embodiment 1, this utility model provides a technical solution: the monitoring sensing component 13 includes a fluorescence sensor and an image acquisition camera.

[0033] Analysis of the above content: The fluorescence sensor and the image acquisition camera can respectively collect fluorescence information and image data, and can also be set as other sensing components according to the needs of use.

[0034] Example 4:

[0035] Please see Figure 1-3 Based on Embodiment 1, this utility model provides a technical solution: a drainage collection box 11 is provided on the upper surface of the horizontal plate of the mounting folding plate 1, and an output pipe 10 is connected to the output microchannel 9. The output pipe 10 is connected to the drainage collection box 11.

[0036] Based on the above analysis, the used cells and liquid culture medium are no longer needed and are collected centrally through the drainage collection box 11.

[0037] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. It is obvious to those skilled in the art that this utility model is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or basic characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description. Therefore, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this utility model, and no reference numerals in the claims should be considered as limiting the scope of the claims.

[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A high-throughput cell culture and real-time monitoring device based on a microfluidic chip, characterized in that, include: Install the folding plate (1), microfluidic chip (7), two storage containers (14) and monitoring sensor assembly (13); The mounting plate (1) includes a horizontal plate and a vertical plate disposed on one side of the horizontal plate. The microfluidic chip (7) is disposed on the upper surface of the horizontal plate of the mounting plate (1). The microfluidic chip (7) is provided with an inlet microchannel (8) and an outlet microchannel (9). There are two inlet microchannels (8). The ends of the two inlet microchannels (8) intersect to form a culture chamber. One end of the outlet microchannel (9) is connected to the culture chamber. Two storage containers (14) are set on the upright plate of the mounting plate (1). The bottom ends of the two storage containers (14) are connected to microfluidic output tubes (15). Microfluidic control pumps (16) are installed on the microfluidic output tubes (15). The other ends of the two microfluidic output tubes (15) are respectively connected to two supply microchannels (8). An installation bracket (12) is set on the upright plate of the mounting plate (1). A monitoring sensor component (13) is installed on the installation bracket (12).

2. The high-throughput cell culture and real-time monitoring device based on a microfluidic chip according to claim 1, characterized in that: The mounting plate (1) has a support groove (2) in the middle of the horizontal plate, and a transparent support plate (3) is embedded in the support groove (2). The microfluidic chip (7) is located on the upper surface of the transparent support plate (3), and a supplementary light device corresponding to the position of the transparent support plate (3) is provided on the lower surface of the mounting plate (1).

3. The high-throughput cell culture and real-time monitoring device based on a microfluidic chip according to claim 2, characterized in that: The supplementary lighting device includes a support plate (4) connected to the lower surface of the mounting plate (1) by a support column, and lighting lamp beads (5) are arranged at equal intervals on the upper surface of the support plate (4).

4. The high-throughput cell culture and real-time monitoring device based on a microfluidic chip according to claim 3, characterized in that: The mounting plate (1) has support bars (6) at both ends of its lower surface, and the bottom end of the support bar (6) is lower than the lower surface of the bearing plate (4).

5. The high-throughput cell culture and real-time monitoring device based on a microfluidic chip according to claim 1, characterized in that: The monitoring sensing component (13) includes a fluorescence sensor and an image acquisition camera.

6. The high-throughput cell culture and real-time monitoring device based on a microfluidic chip according to claim 1, characterized in that: The upper surface of the horizontal plate of the mounting plate (1) is provided with a drainage collection box (11), and the output microchannel (9) is connected to the output pipe (10), which is connected to the drainage collection box (11).

Images