Protein separation and purification device convenient to adjust

Abstract

The utility model discloses a protein separation and purification device convenient to adjust, and relates to the technical field of protein separation and purification devices. The device comprises a processing box, a dismounting mechanism is arranged on the processing box, and the dismounting mechanism comprises a filtering cavity formed in the processing box. According to the utility model, the separation screen plate can be dismounted and mounted through the dismounting mechanism, so that an operator can flexibly replace the separation screen plate with adaptive aperture or material according to different protein sample characteristics and separation and purification requirements, the filtering effect is optimized, the accuracy and efficiency of the whole separation and purification process are improved, and meanwhile, the separation and purification efficiency is improved. The detachable separation net plate greatly facilitates maintenance work, after the device is used, the separation net plate can be conveniently taken out, comprehensive and thorough cleaning is carried out, accumulated impurities and residual protein are effectively removed, the filter holes are prevented from being blocked by the impurities and the residual protein, it is guaranteed that the separation net plate continuously and stably plays a filtering function, and the service life of the separation net plate is prolonged.

Description

Technical Field

[0001] This invention belongs to the technical field of protein separation and purification devices, and in particular relates to a protein separation and purification device that is easy to adjust. Background Technology

[0002] The related technology discloses a protein separation and purification device with announcement number CN217939405U. The device uses a separation screen plate that slides up and down to make the separation screen plate vibrate, which facilitates the further separation of impurities inside the protein during separation. This improves the efficiency of the separation and purification device, effectively increases the separation speed, and is practical and easy to use.

[0003] However, the separation screen of this device is not easy to disassemble during use. As a result, when faced with different protein sample characteristics and separation and purification requirements, operators cannot flexibly change the separation screen with appropriate pore size or material. This makes it difficult to optimize the filtration effect and greatly reduces the accuracy and efficiency of the entire separation and purification process. Utility Model Content

[0004] The purpose of this invention is to provide a protein separation and purification device that is easy to adjust. By providing a disassembly mechanism, the problem of the separation screen plate being inconvenient to disassemble during use is solved.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] This invention relates to an easily adjustable protein separation and purification device, comprising a processing chamber equipped with a disassembly mechanism and a stirring mechanism.

[0007] The disassembly mechanism includes a filter chamber located inside the processing box. A mounting frame is slidably connected to the front side of the processing box, and the rear side of the mounting frame extends into the filter chamber. A separation mesh plate is slidably connected to the inner wall of the mounting frame. T-shaped sliders are fixedly connected to the left and right sides of the mounting frame. T-shaped grooves are provided on the left and right inner walls of the filter chamber. Both T-shaped sliders are slidably connected to their corresponding T-shaped grooves. The stirring mechanism includes a support plate fixedly connected to the top of the processing box.

[0008] Furthermore, a C-shaped plate is fixedly connected to the front side of the processing box, and a bidirectional threaded rod passes through the C-shaped plate. The bidirectional threaded rod is rotatably connected to the C-shaped plate, and a knob is fixedly connected to the right end of the bidirectional threaded rod.

[0009] Furthermore, the bidirectional threaded rod is threaded with two internal threaded blocks, the inner wall of the C-shaped plate is fixedly connected with a slide rod, both internal threaded blocks are slidably connected to the slide rod, the inner wall of the C-shaped plate is slidably connected to a moving plate, and both internal threaded blocks are hinged to the moving plate with a connecting plate.

[0010] Furthermore, the mounting frame has two positioning slots, and the bottom of the movable plate is fixedly connected to two positioning blocks. The bottom of each positioning block extends into the interior of the corresponding positioning slot and is slidably connected to the corresponding positioning slot. T-shaped sliders are fixedly connected to the left and right sides of the movable plate. T-shaped grooves are provided on the left and right inner walls of the U-shaped plate. The two T-shaped sliders are slidably connected to the corresponding T-shaped grooves.

[0011] Furthermore, the processing box has a stirring chamber inside, and a motor is fixedly connected to the top of the support plate. The output shaft of the motor is fixedly connected to a rotating shaft 1 via a coupling. The bottom end of the rotating shaft 1 extends into the interior of the stirring chamber and is rotatably connected to the processing box and the support plate.

[0012] Furthermore, the top of the processing box is rotatably connected to several rotating shafts, the bottom ends of which extend into the interior of the stirring chamber. A gear is fixedly connected to the rotating shaft, and gears are fixedly connected to the rotating shafts. All gears mesh with gears.

[0013] Furthermore, several stirring rods are fixedly connected to the rotating shaft one, and several stirring rods are fixedly connected to the rotating shaft two.

[0014] Furthermore, a connecting pipe is fixedly connected to the inner top wall of the filter chamber, a valve is fixedly connected to the outer wall of the connecting pipe, a feeding pipe is fixedly connected to the outer wall of the processing box, and a discharging pipe is fixedly connected to the bottom of the processing box.

[0015] This utility model has the following beneficial effects:

[0016] 1. By setting up a disassembly mechanism, rotating the knob in the opposite direction will drive the bidirectional threaded rod to rotate. When the bidirectional threaded rod rotates, it drives the internal threaded block to move. When the internal threaded block moves, it drives the connecting plate to move. When the connecting plate moves, it drives the moving plate to move. When the moving plate moves, it drives the positioning block to disengage from the positioning groove. The disassembly mechanism allows the separation screen plate to be disassembled and installed. This allows operators to flexibly change the separation screen plate with appropriate pore size or material according to the characteristics of different protein samples and separation and purification needs, thereby optimizing the filtration effect and improving the accuracy and efficiency of the entire separation and purification process. At the same time, the detachable separation screen plate greatly facilitates maintenance. After the device is used, the separation screen plate can be easily removed for thorough cleaning, effectively removing accumulated impurities and residual proteins, preventing them from clogging the filter pores, ensuring that the separation screen plate can continuously and stably perform its filtration function, and extending the service life of the separation screen plate.

[0017] 2. By setting up a stirring mechanism and starting the motor, the motor drives shaft one to rotate, which in turn drives gear one to rotate, which in turn drives gear two to rotate, which in turn drives shaft two to rotate. The rotation of shaft one and shaft two respectively drives stirring rod one and stirring rod two to rotate. Through the stirring mechanism, the raw materials in the stirring chamber are mixed and stirred, so that the components in the raw materials are fully mixed, and the protein and related reagents are in uniform contact, which accelerates the chemical reaction process, improves the reaction efficiency, and promotes the effective extraction and separation of proteins. At the same time, stirring can prevent precipitation or stratification of different components in the raw materials due to density differences, maintain the homogeneity of the system, ensure the stability and accuracy of subsequent operations such as filtration, and ensure the efficient and stable operation of the entire protein separation and purification process, ultimately improving the purity and yield of the target protein.

[0018] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the front cross-section of the present invention;

[0022] Figure 3 This is a schematic diagram of the structure of the C-shaped plate of this utility model;

[0023] Figure 4 This is a schematic diagram of the installation frame of this utility model;

[0024] Figure 5 This is a schematic diagram of the structure of the motor of this utility model.

[0025] The attached diagram lists the components represented by each number as follows:

[0026] 1. Processing box; 2. Disassembly mechanism; 3. Stirring mechanism; 21. Filter chamber; 22. Mounting frame; 23. Separation screen plate; 24. T-shaped slider one; 25. T-shaped slide groove one; 26. C-shaped plate; 27. Bidirectional threaded rod; 28. Knob; 29. ​​Internal threaded block; 210. Slide rod; 211. Moving plate; 212. Connecting plate; 213. Positioning groove; 214. Positioning block; 215. T-shaped slider two; 216. T-shaped slide groove two; 31. Support plate; 32. Stirring chamber; 33. Motor; 34. Rotating shaft one; 35. Rotating shaft two; 36. Gear one; 37. Gear two; 38. Stirring rod one; 39. Stirring rod two; 310. Connecting pipe; 311. Valve; 312. Feeding pipe; 313. Discharging pipe. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0028] Please see Figure 1-5As shown, this utility model is an easily adjustable protein separation and purification device, including a processing box 1. The processing box 1 is equipped with a disassembly mechanism 2 and a stirring mechanism 3. The disassembly mechanism 2 includes a filter chamber 21 opened inside the processing box 1. A mounting frame 22 is slidably connected to the front side of the processing box 1. The rear side of the mounting frame 22 extends into the interior of the filter chamber 21. A separation mesh plate 23 is slidably connected to the inner wall of the mounting frame 22. T-shaped sliders 24 are fixedly connected to the left and right sides of the mounting frame 22. T-shaped grooves 25 are opened on the left and right inner walls of the filter chamber 21. Both T-shaped sliders 24 are... The stirring mechanism 3 includes a support plate 31 fixedly connected to the top of the processing box 1, a U-shaped plate 26 fixedly connected to the front side of the processing box 1, a bidirectional threaded rod 27 passing through the U-shaped plate 26, the bidirectional threaded rod 27 being rotatably connected to the U-shaped plate 26, a knob 28 fixedly connected to the right end of the bidirectional threaded rod 27, two internal threaded blocks 29 threadedly connected to the bidirectional threaded rod 27, a slide rod 210 fixedly connected to the inner wall of the U-shaped plate 26, both internal threaded blocks 29 being slidably connected to the slide rod 210, a movable plate 211 slidably connected to the inner wall of the U-shaped plate 26, and the two internal threaded blocks... 29 is hinged to the movable plate 211 by a connecting plate 212. The mounting frame 22 has two positioning grooves 213. Two positioning blocks 214 are fixedly connected to the bottom of the movable plate 211. The bottoms of the two positioning blocks 214 extend into the corresponding positioning grooves 213 and slide in connection with them. T-shaped sliders 215 are fixedly connected to the left and right sides of the movable plate 211. T-shaped grooves 216 are provided on the left and right inner walls of the U-shaped plate 26. The two T-shaped sliders 215 slide in connection with their corresponding T-shaped grooves 216. The disassembly mechanism 2 allows for separation. The screen plate 23 can be disassembled and installed, allowing operators to flexibly replace the screen plate 23 with one of suitable pore size or material according to the characteristics of different protein samples and the requirements of separation and purification. This optimizes the filtration effect and improves the accuracy and efficiency of the entire separation and purification process. At the same time, the detachable screen plate 23 greatly facilitates maintenance. After the device is used, the screen plate 23 can be easily removed for thorough cleaning, effectively removing accumulated impurities and residual proteins, preventing them from clogging the filter pores, ensuring that the screen plate 23 can continuously and stably perform its filtration function, and extending the service life of the screen plate 23.The processing box 1 has an internal stirring chamber 32. A motor 33 is fixedly connected to the top of the support plate 31. The output shaft of the motor 33 is fixedly connected to a rotating shaft 34 via a coupling. The bottom end of the rotating shaft 34 extends into the interior of the stirring chamber 32 and is rotatably connected to the processing box 1 and the support plate 31. Several rotating shafts 35 are rotatably connected to the top of the processing box 1. The bottom ends of the rotating shafts 35 all extend into the interior of the stirring chamber 32. A gear 36 is fixedly connected to the rotating shaft 34. A gear 37 is fixedly connected to each of the rotating shafts 35. The gears 37 mesh with the gears 36. Several stirring rods 38 are fixedly connected to the rotating shaft 34. Several stirring rods 39 are fixedly connected to each of the rotating shafts 35. The filter chamber 21 contains... A connecting pipe 310 is fixedly connected to the top wall, and a valve 311 is fixedly connected to the outer wall of the connecting pipe 310. A feeding pipe 312 is fixedly connected to the outer wall of the processing chamber 1, and a discharging pipe 313 is fixedly connected to the bottom of the processing chamber 1. The raw materials in the stirring chamber 32 are mixed and stirred by the stirring mechanism 3, so that the components in the raw materials are fully mixed, the protein and related reagents are uniformly contacted, the chemical reaction process is accelerated, the reaction efficiency is improved, and the effective extraction and separation of proteins are promoted. At the same time, stirring can prevent precipitation or stratification of different components in the raw materials due to density differences, maintain the homogeneity of the system, ensure the stability and accuracy of subsequent operations such as filtration, ensure the efficient and stable operation of the entire protein separation and purification process, and ultimately improve the purity and yield of the target protein.

[0029] A specific application of this embodiment is as follows: When using this device, after the raw material is stirred by the stirring mechanism 3, it enters the filter chamber 21 from the stirring chamber 32. After entering the filter chamber 21, the raw material falls onto the separating screen plate 23, which filters the raw material and separates the impurities. The raw material after impurity separation is discharged through the feed pipe 313 for subsequent production processes. When it is necessary to disassemble the separating screen plate 23 for cleaning or maintenance, first turn the knob 28 in the reverse direction. The knob 28 will drive the bidirectional threaded rod 27 to rotate. The internal thread block 29 moves as the sliding rod 210 limits its movement, converting its rotational motion into linear motion. The internal thread block 29 moves, causing the connecting plate 212 to move, which in turn moves the moving plate 211. The moving plate 211 then moves the T-shaped slider 215 within the T-shaped groove 216. The T-shaped slider 215 and the T-shaped groove 216 stabilize the movement of the moving plate 211. During movement, the moving plate 211 causes the positioning block 214 to disengage from the positioning groove 213. When the positioning block 214 is completely disengaged... After disengaging from the positioning groove 213, pull the mounting frame 22 forward to detach it from the processing box 1. Since the separating screen plate 23 is slidably connected to the inner wall of the mounting frame 22, the separating screen plate 23 can be disassembled once the mounting frame 22 is completely detached from the processing box 1. The bottom of the mounting frame 22 has a protruding horizontal plate, so there is no need to worry about the separating screen plate 23 falling out of the mounting frame 22. When it is necessary to install the separating screen plate 23, first place the separating screen plate 23 into the interior of the mounting frame 22, and then slide the mounting frame 22 into the interior of the filter chamber 21, while simultaneously allowing the T-shaped slider 24 to slide into the corresponding T-shaped... Inside the slide groove 25, the T-shaped slider 24 and the T-shaped slide groove 25 are used to position and guide the mounting frame 22. The end of the mounting frame 22 with the separation mesh plate 23 faces upward and the end with the separation mesh plate 23 is on the outside of the processing box 1. When the mounting frame 22 slides into the processing box 1, the knob 28 is rotated in the forward direction. The principle of the forward rotation of the knob 28 is the same as the principle of the reverse rotation. Therefore, when the knob 28 is rotated in the forward direction, the positioning block 214 will move into the positioning groove 213, thereby completing the fixation of the mounting frame 22 and preventing the separation mesh plate 23 from moving when the mounting frame 22 moves in case of accident.

[0030] The raw materials are fed into the mixing chamber 32 through the feeding pipe 312. The motor 33 is started, and the motor 33 drives the rotating shaft 34 to rotate. When the rotating shaft 34 rotates, it drives the gear 36 to rotate. When the gear 36 rotates, it drives the gear 37 to rotate. When the gear 37 rotates, it drives the rotating shaft 35 to rotate. When the rotating shaft 34 and the rotating shaft 35 rotate, they drive the stirring rod 38 and the stirring rod 39 to rotate, respectively. Thus, the raw materials in the mixing chamber 32 are stirred by the stirring rods 38 and 39. After the raw materials are stirred, the valve 311 is opened, so that the raw materials in the mixing chamber 32 enter the filter chamber 21 through the connecting pipe 310, and are filtered by the separation screen 23.

[0031] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0032] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A protein separation and purification device that is easy to adjust, characterized in that: It includes a processing box (1), and the processing box (1) is provided with a disassembly mechanism (2) and a stirring mechanism (3); The disassembly mechanism (2) includes a filter chamber (21) opened inside the processing box (1). The front side of the processing box (1) is slidably connected to an installation frame (22). The rear side of the installation frame (22) extends into the interior of the filter chamber (21). The inner wall of the installation frame (22) is slidably connected to a separation mesh plate (23). The left and right sides of the installation frame (22) are fixedly connected to T-shaped sliders (24). The left and right inner walls of the filter chamber (21) are provided with T-shaped grooves (25). The two T-shaped sliders (24) are slidably connected to the corresponding T-shaped grooves (25). The stirring mechanism (3) includes a support plate (31) fixedly connected to the top of the processing box (1). A U-shaped plate (26) is fixedly connected to the front side of the processing box (1). A bidirectional threaded rod (27) passes through the U-shaped plate (26). The bidirectional threaded rod (27) is rotatably connected to the U-shaped plate (26). A knob (28) is fixedly connected to the right end of the bidirectional threaded rod (27). The bidirectional threaded rod (27) is threaded with two internal threaded blocks (29). The inner wall of the swivel plate (26) is fixedly connected with a slide rod (210). Both internal threaded blocks (29) are slidably connected to the slide rod (210). The inner wall of the swivel plate (26) is slidably connected with a moving plate (211). Both internal threaded blocks (29) are hinged to the moving plate (211) with a connecting plate (212). The mounting frame (22) has two positioning slots (213). The bottom of the movable plate (211) is fixedly connected to two positioning blocks (214). The bottom of the two positioning blocks (214) extends into the interior of the corresponding positioning slot (213) and slides in connection with the corresponding positioning slot (213). The left and right sides of the movable plate (211) are fixedly connected to T-shaped sliders (215). The inner walls of the left and right sides of the U-shaped plate (26) are provided with T-shaped grooves (216). The two T-shaped sliders (215) slide in connection with the corresponding T-shaped grooves (216).

2. The easily adjustable protein separation and purification device according to claim 1, characterized in that, The processing box (1) has a stirring chamber (32) inside. A motor (33) is fixedly connected to the top of the support plate (31). The output shaft of the motor (33) is fixedly connected to a rotating shaft (34) through a coupling. The bottom end of the rotating shaft (34) extends into the stirring chamber (32) and is rotatably connected to the processing box (1) and the support plate (31).

3. The easily adjustable protein separation and purification device according to claim 2, characterized in that, The top of the processing box (1) is rotatably connected to several rotating shafts (35), the bottom ends of several rotating shafts (35) extend into the interior of the stirring chamber (32), a gear (36) is fixedly connected to the rotating shaft (34), a gear (37) is fixedly connected to several rotating shafts (35), and several gears (37) mesh with gears (36).

4. The easily adjustable protein separation and purification device according to claim 3, characterized in that, Several stirring rods (38) are fixedly connected to the rotating shaft one (34), and several stirring rods (39) are fixedly connected to the rotating shaft two (35).

5. The easily adjustable protein separation and purification apparatus according to claim 4, characterized in that, The inner top wall of the filter chamber (21) is fixedly connected to a connecting pipe (310), the outer wall of the connecting pipe (310) is fixedly connected to a valve (311), the outer wall of the processing box (1) is fixedly connected to a feeding pipe (312), and the bottom of the processing box (1) is fixedly connected to a discharging pipe (313).

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