Lactic acid bacteria fermentation liquor separation device

Abstract

The utility model discloses a lactic acid bacteria fermentation liquor separation device which comprises a cyclone separation cavity device for primarily separating thalli from supernate and a filtering module for finely filtering the primarily separated fermentation liquor, and the cyclone separation cavity device is connected with the filtering module through a corresponding pipeline. The filtering module comprises a filtering box, a flexible membrane assembly, a vibration driving mechanism and a back flushing mechanism; the flexible membrane assembly is arranged in the filtering box and is used for finely filtering the primarily separated fermentation liquid; the vibration driving mechanism is used for driving the flexible membrane assembly to generate micro-amplitude vibration; the back flushing mechanism is used for generating shock waves to vibrate off accumulated thalli on the flexible membrane assembly. According to the utility model, the accumulation of filter cakes is prevented, the problem of frequent shutdown for cleaning the filter screen is effectively avoided, the continuous and stable proceeding of the filtering process is ensured, the separation efficiency of lactic acid bacteria fermentation liquor is remarkably improved, the utilization rate of production equipment is improved, and the production cost is reduced.

Description

Technical Field

[0001] This utility model belongs to the field of fermentation broth separation technology, specifically, it relates to a lactic acid bacteria fermentation broth separation device. Background Technology

[0002] Lactic acid bacteria, as an important probiotic, have wide applications in food, medicine, agriculture, and other fields. However, in the actual application of lactic acid bacteria fermentation broth, in addition to containing a large number of lactic acid bacteria cells, the fermentation broth also contains unutilized culture medium components, metabolites, and some impurities. The presence of these impurities not only affects the purity and quality of the product but may also adversely affect subsequent processing, storage, and use.

[0003] Currently, a prominent problem in the separation of lactic acid bacteria fermentation broth is the frequent occurrence of filter clogging due to bacterial buildup. As the filtration process proceeds, a large number of lactic acid bacteria gradually accumulate on the filter surface, forming a thick filter cake layer. This filter cake layer not only increases filtration resistance, gradually slowing down the filtration speed, but also, when it accumulates to a certain extent, completely blocks the pores of the filter, causing filtration to cease. Once this happens, the filtration equipment must be stopped, and the filter screen cleaned to remove the bacteria and impurities clogging it. Frequent shutdowns for cleaning significantly reduce production efficiency and increase production costs. Utility Model Content

[0004] In order to solve the problems existing in the prior art, this utility model aims to provide a lactic acid bacteria fermentation broth separation device, so as to effectively avoid the phenomenon of bacterial accumulation clogging the filter screen, ensure the continuous and stable operation of the filtration process, significantly improve the separation efficiency of lactic acid bacteria fermentation broth, and reduce the increase in production costs caused by frequent shutdowns to clean the filter screen.

[0005] To achieve the above-mentioned technical objectives and effects, this utility model is implemented through the following technical solution:

[0006] A lactic acid bacteria fermentation broth separation device includes a cyclone separation chamber for initial separation of bacteria and supernatant, and a filtration module for fine filtration of the initially separated fermentation broth. The cyclone separation chamber and the filtration module are connected by corresponding pipes. The filtration module includes a filter box, a flexible membrane assembly disposed in the filter box for fine filtration of the initially separated fermentation broth, a vibration drive mechanism for driving the flexible membrane assembly to generate micro-vibrations, and a backwash mechanism for generating shock waves to shake off the bacteria accumulated on the flexible membrane assembly.

[0007] Furthermore, the flexible membrane assembly is made of polytetrafluoroethylene nanofibers.

[0008] Furthermore, the flexible membrane assembly has a gradient pore size distribution and its surface is coated with a chitosan-sodium alginate composite coating.

[0009] Furthermore, the vibration drive mechanism employs a piezoelectric ceramic sheet.

[0010] Furthermore, the recoil mechanism includes a high-pressure gas storage tank and a recoil execution module, which is connected via corresponding pipelines.

[0011] Furthermore, the backflush execution module includes an electromagnetic pulse valve and a jet pipe. The outlet of the electromagnetic pulse valve is connected to the jet pipe through a corresponding pipe, while the inlet of the electromagnetic pulse valve is connected to the high-pressure gas storage tank through a corresponding pipe.

[0012] Furthermore, the backflush execution module also includes a pressure sensor, which monitors the pressure difference between the inside and outside of the flexible membrane assembly.

[0013] Furthermore, the cyclone separation chamber device adopts a three-stage cyclone separation chamber structure.

[0014] Furthermore, it also includes a temperature circulation system for maintaining the temperature environment during the separation process.

[0015] Furthermore, the temperature circulation system includes a plate heat exchanger and heat exchange pipes.

[0016] The beneficial effects of this utility model are as follows:

[0017] 1. This utility model uses a cyclone separation chamber device to perform three-stage cyclone separation on the fermentation broth, initially separating bacteria of different particle sizes from the supernatant. This reduces the pressure on the subsequent filtration module. At the same time, by setting a vibration drive mechanism and a backwash mechanism in the filtration module, filter cake accumulation is prevented, effectively avoiding the problem of frequent shutdowns to clean the filter screen. This ensures that the filtration process is continuous and stable, significantly improving the separation efficiency of lactic acid bacteria fermentation broth, increasing the utilization rate of production equipment, and reducing production costs.

[0018] 2. By setting up a temperature circulation system, this utility model maintains the temperature environment throughout the separation process, which helps to maintain the activity of lactic acid bacteria, reduces damage to the bacteria caused by temperature changes, and further ensures the quality of the product.

[0019] The above description is merely an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it according to the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. The specific implementation methods of this utility model are given in detail in the following embodiments and their accompanying drawings. Attached Figure Description

[0020] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0021] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0022] Figure 2 This is a schematic diagram of the filter module of this utility model.

[0023] The following are the labels in the diagram: 1. Cyclone separation chamber device; 11. Primary cyclone chamber; 12. Secondary cyclone chamber; 13. Tertiary cyclone chamber; 2. Filter module; 21. Filter box; 211. Recovery port; 22. Flexible membrane module; 23. Vibration drive mechanism; 24. Backflush mechanism; 241. High-pressure air tank; 242. Backflush actuator module; 2421. Electromagnetic pulse valve; 2422. Jet pipe; 3. Temperature circulation system; 31. Plate heat exchanger; 32. Heat exchange pipeline; 33. Circulation pump; 4. Outer shell; 5. Insulation material; 6. Electromagnetically controlled check valve. Detailed Implementation

[0024] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0025] It should be noted that all directional indicators (such as up, down, left, right, front, back, upper end, lower end, top, bottom, etc.) in this utility model embodiment are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0026] See Figure 1-2 As shown, a lactic acid bacteria fermentation broth separation device includes a cyclone separation chamber device 1 for preliminary separation of bacteria and supernatant, and a filter module 2 for fine filtration of the fermentation broth after preliminary separation. The cyclone separation chamber device 1 and the filter module 2 are connected by corresponding pipes.

[0027] In this embodiment, the cyclone separation chamber device 1 includes a primary cyclone chamber 11, a secondary cyclone chamber 12, and a tertiary cyclone chamber 13, forming a three-stage cyclone separation chamber structure. During the separation operation, the fermentation broth sequentially enters the primary cyclone chamber 11, the secondary cyclone chamber 12, and the tertiary cyclone chamber 13. In the primary cyclone chamber 11, medium and large particle bacteria (diameter > 50 μm) are separated; in the secondary cyclone chamber 12, small and medium-sized particle bacteria (10-50 μm) are separated; and in the tertiary cyclone chamber 13, tiny bacterial cells (< 10 μm) are separated.

[0028] In this embodiment, the filtration module 2 includes a filter box 21, a flexible membrane assembly 22 disposed in the filter box 21 for fine filtration of the fermentation broth after preliminary separation, a vibration drive mechanism 23 for driving the flexible membrane assembly 22 to generate micro-vibrations, and a backwash mechanism 24 for generating shock waves to shake off the bacteria accumulated on the flexible membrane assembly 22.

[0029] In this embodiment, a recovery port 211 is provided on the lower side wall of the filter box 21. The recovery port 211 is in a sealed closed state during the separation operation, and the residual bacteria trapped in the fermentation broth can be easily removed through the recovery port 211. The flexible membrane component 22 is made of polytetrafluoroethylene (PTFE) nanofibers with a gradient pore size distribution (0.2-1.0μm) and is coated with a chitosan-sodium alginate composite coating to reduce bacterial adsorption.

[0030] The vibration drive mechanism 23 uses a piezoelectric ceramic sheet and is located below the flexible membrane assembly 22. The piezoelectric ceramic sheet drives the flexible membrane assembly 22 to generate micro-vibrations (amplitude ±0.5mm, frequency 200Hz), thereby preventing filter cake accumulation on the flexible membrane assembly 22 and affecting the separation efficiency.

[0031] The backflushing mechanism 24 includes a high-pressure gas storage tank 241 and a backflushing execution module 242, which is connected by corresponding pipelines. During the separation operation, nitrogen gas is supplied to the backflushing execution module 242 through the high-pressure gas storage tank 241 to generate shock waves that dislodge the bacteria accumulated on the flexible membrane assembly 22, thereby effectively avoiding the problem of reduced production efficiency caused by frequent shutdowns for cleaning during the separation operation.

[0032] The recoil actuator 242 includes an electromagnetic pulse valve 2421 and a jet pipe 2422. During connection, the outlet of the electromagnetic pulse valve 2421 is connected to the jet pipe 2422 via a corresponding pipe, while the inlet of the electromagnetic pulse valve 2421 is connected to the high-pressure gas storage tank 241 via a corresponding pipe. During operation, the electromagnetic pulse valve 2421 controls the on / off state of the high-pressure gas and the jetting rhythm. In this embodiment, to achieve precise control of the on / off state of the electromagnetic pulse valve 2421, the recoil actuator 242 also includes a pressure... A sensor (not shown in the figure) detects the pressure difference between the inside and outside of the flexible membrane assembly 22 via the pressure sensor. When the pressure sensor detects that the transmembrane pressure difference is greater than a set pressure (in this embodiment, it is set to >0.1MPa), it controls the electromagnetic pulse valve 2421 to start, causing nitrogen gas in the high-pressure gas storage tank 241 to be sprayed out through the spray pipe 2422, thereby backflushing the flexible membrane assembly 22 and shaking off the bacteria accumulated on the flexible membrane assembly 22. In this embodiment, the nitrogen backflushing pressure is 0.3MPa and the pulse width is 50ms.

[0033] Furthermore, in this embodiment, the device also includes a temperature circulation system 3 for maintaining the temperature environment during the separation process. The temperature circulation system 3 includes a plate heat exchanger 31, heat exchange pipes 32, and a circulation pump 33. During installation, the heat exchange pipes 32 are wound around the outer surfaces of the primary cyclone chamber 11, the secondary cyclone chamber 12, the tertiary cyclone chamber 13, and the filter box 21. After winding, an outer shell 4 is wrapped around the outside. A sealed insulation layer is formed by wrapping the outer shell 4 and filling it with insulation material 5 (such as polyurethane foam or aerogel). Specifically, as shown... Figure 2 As shown, it should be noted that the primary swirl chamber 11, the secondary swirl chamber 12, and the tertiary swirl chamber 13 can be set up with reference to the filter box 21, and will not be shown again; in addition, it should be noted that in this embodiment, in order to achieve individual temperature control, electromagnetic control check valves 6 are respectively installed on the heat exchange pipes 32 upstream of the inlet of the primary swirl chamber 11, the secondary swirl chamber 12, the tertiary swirl chamber 13, and the filter box 21, and on the heat exchange pipes 32 downstream of the outlet of the secondary swirl chamber 12 and the tertiary swirl chamber 13.

[0034] The working principle of this utility model is as follows:

[0035] Before the separation operation, the device is connected to the control system. The control system monitors the flow rate, pressure and temperature parameters in real time through the PLC controller, and automatically adjusts the rotation speed of the cyclone separation chamber device 1, the vibration frequency of the vibration drive mechanism 23 and the backwash cycle of the backwash mechanism 24.

[0036] During the separation process, the fermentation broth enters the hydrocyclone separation chamber 1, where a three-stage hydrocyclone separation process is performed on the bacterial cells of different particle sizes in the fermentation broth. After the bacterial cells are initially separated from the supernatant, the broth enters the filter box 21 of the filter module 2 through the corresponding pipe. The fermentation broth is then finely filtered by the flexible membrane assembly 22 to retain any residual bacterial cells in the fermentation broth, thus completing the separation of the fermentation broth.

[0037] During the separation process, the vibration drive mechanism 23 (piezoelectric ceramic plate) works continuously, driving the flexible membrane assembly 22 to vibrate continuously, thereby preventing filter cake accumulation on the flexible membrane assembly 22, ensuring the smooth progress of the filtration process, and maintaining high separation efficiency. Then, the piezoelectric ceramic plate drives the flexible membrane assembly 22 to generate micro-vibrations, which can prevent filter cake accumulation. However, under continuous operation, filter cake accumulation is unavoidable. At this time, when the pressure sensor detects that the pressure difference between the inside and outside of the flexible membrane assembly 22 is greater than the set pressure, the control system controls the backwash mechanism 24 to start, generating a shock wave to dislodge the accumulated bacteria on the flexible membrane assembly 22, thus effectively avoiding the problem of reduced production efficiency caused by frequent shutdowns for cleaning during the separation process.

[0038] In addition, during the separation process, the plate heat exchanger 31 exchanges heat with the circulating medium. After heat exchange, the medium enters the heat exchange pipe 32 under the action of the circulating pump 33. When the medium flows through the first-stage cyclone chamber 11, the second-stage cyclone chamber 12, the third-stage cyclone chamber 13 and the outer surface of the filter box 21, it exchanges heat with them and then flows back to the plate heat exchanger 31 to form a cycle, thereby maintaining the temperature environment of the entire separation process.

[0039] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A lactic acid bacteria fermentation broth separation device, comprising a cyclone separation chamber device (1) for preliminary separation of bacteria and supernatant, and a filter module (2) for fine filtration of the fermentation broth after preliminary separation, wherein the cyclone separation chamber device (1) and the filter module (2) are connected by corresponding pipes, characterized in that: The filtration module (2) includes a filter box (21), a flexible membrane assembly (22) disposed in the filter box (21) for fine filtration of the fermentation broth after preliminary separation, a vibration drive mechanism (23) for driving the flexible membrane assembly (22) to generate micro-vibrations, and a backwash mechanism (24) for generating shock waves to shake off the bacteria accumulated on the flexible membrane assembly (22).

2. The lactic acid bacteria fermentation broth separation device according to claim 1, characterized in that: The flexible membrane assembly (22) is made of polytetrafluoroethylene nanofibers.

3. The lactic acid bacteria fermentation broth separation device according to claim 2, characterized in that: The flexible membrane assembly (22) has a gradient pore size and is coated with a chitosan-sodium alginate composite coating.

4. The lactic acid bacteria fermentation broth separation device according to claim 1, characterized in that: The vibration drive mechanism (23) uses a piezoelectric ceramic sheet.

5. The lactic acid bacteria fermentation broth separation device according to claim 1, characterized in that: The recoil mechanism (24) includes a high-pressure gas storage tank (241) and a recoil execution module (242), which is connected by a corresponding pipeline.

6. The lactic acid bacteria fermentation broth separation device according to claim 5, characterized in that: The recoil actuator module (242) includes an electromagnetic pulse valve (2421) and a blow pipe (2422). The outlet of the electromagnetic pulse valve (2421) is connected to the blow pipe (2422) through a corresponding pipe, while the inlet of the electromagnetic pulse valve (2421) is connected to the high-pressure gas storage tank (241) through a corresponding pipe.

7. The lactic acid bacteria fermentation broth separation device according to claim 6, characterized in that: The recoil actuator module (242) also includes a pressure sensor, which monitors the pressure difference between the inside and outside of the flexible membrane assembly (22).

8. The lactic acid bacteria fermentation broth separation device according to claim 1, characterized in that: The cyclone separation chamber device (1) adopts a three-stage cyclone separation chamber structure.

9. The lactic acid bacteria fermentation broth separation device according to claim 1, characterized in that: It also includes a temperature circulation system (3) for maintaining the temperature environment of the separation process.

10. The lactic acid bacteria fermentation broth separation device according to claim 9, characterized in that: The temperature circulation system (3) includes a plate heat exchanger (31) and heat exchange pipes (32).

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