3D cell culture plate

Abstract

The utility model belongs to the technical field of 3D cell culture, and discloses a 3D cell culture plate which comprises a plate body, a plurality of reaction tanks are arranged on the plate body, and the plurality of reaction tanks are distributed in an adjacent array; a sample adding hole and a plurality of reaction holes are formed in the bottom of each reaction tank, and the plurality of reaction holes are arranged around the circumferential direction of the sample adding hole. A culture medium can be sucked, discarded and added through the sample adding hole formed in the middle, so that the culture medium is collected from the sample adding hole to be sucked and discarded in the suction and discarding culture process; and in the process of adding the culture medium, the culture medium is diffused from the sample adding hole to the surrounding reaction holes, so that the interference on cells in the processes of absorbing and abandoning the culture medium and adding the culture medium is reduced, the efficiency is improved, the data stability is improved, and meanwhile, the plurality of reaction tanks can also meet the simultaneous culture of a plurality of cell modules.

Description

Technical Field

[0001] This utility model relates to the field of 3D cell culture technology, and in particular to a 3D cell culture plate. Background Technology

[0002] 3D cell culture is a technique that allows biological cells to grow in all three dimensions within an artificially created environment. Unlike 2D environments (such as culture dishes), 3D cell culture allows cells to grow in all directions in vitro, similar to their growth patterns in vivo. These three-dimensional cultures are typically grown in bioreactors or small capsules, where cells can grow into spherical or three-dimensional cell colonies. However, in current 3D cell culture techniques, the early stages are often completed in multi-well plates (such as 96-well or 384-well plates). The culture process typically involves aspirating and adding culture medium to the wells, which can easily interfere with the placed cells, affecting both the efficiency of medium aspiration and addition, and the accuracy of subsequent data analysis. Utility Model Content

[0003] The purpose of this invention is to provide a 3D cell culture plate that reduces interference to cells during the process of aspirating and adding culture medium, thereby improving efficiency and data stability.

[0004] To achieve this objective, the present invention adopts the following technical solution:

[0005] 3D cell culture plate, including the plate body.

[0006] The plate is provided with a plurality of reaction tanks, which are arranged in an adjacent array.

[0007] Each of the reaction tanks has a sample loading hole and several reaction holes on its bottom wall, with the reaction holes arranged circumferentially around the sample loading hole.

[0008] Preferably, the sample feeding hole is cylindrical.

[0009] Preferably, the top diameter of the sample dispensing hole is 1.5mm-2.5mm; the bottom diameter of the sample dispensing hole is 1.5mm-2.5mm; and the height of the sample dispensing hole is 1.5mm-2.5mm.

[0010] Preferably, the reaction orifice includes a top, a middle, and a bottom that are connected in sequence; the top of the orifice is rectangular; the middle of the orifice is an inverted cone; and the bottom of the orifice is hemispherical.

[0011] Preferably, the top of the hole has a length, width, and height of 2.5 mm; the middle of the hole has a height of 1 mm; and the bottom of the hole has a diameter of 1 mm to 3 mm.

[0012] Preferably, the sample feeding holes are arranged in a rectangular array within the reaction vessel.

[0013] Preferably, a plurality of the reaction tanks are arranged in a rectangular array on the plate.

[0014] Preferably, the reaction tank is rectangular.

[0015] Preferably, the length and width of the reaction tank are both 10mm-30mm, and the height of the reaction tank is 3mm-10mm.

[0016] Preferably, the system also includes a cover plate, the outer periphery of which is provided with an insertion step. The cover plate is inserted into the top of the plate to open or close the reaction tank.

[0017] The beneficial effects of this utility model are:

[0018] The sample wells located in the middle are used for both aspiration and addition of culture medium. During the aspiration process, the culture medium flows into the sample wells for aspiration, while during the addition process, the culture medium diffuses from the sample wells to the surrounding reaction wells. This reduces interference with cells during aspiration and addition, improves efficiency, and enhances data stability. In addition, multiple reaction tanks can accommodate the simultaneous culture of multiple cell modules. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the plate body in the 3D cell culture plate of this utility model;

[0020] Figure 2 This is a schematic diagram of one of the reaction tanks of this utility model;

[0021] Figure 3 This is a side view of the plate body in the 3D cell culture plate of this utility model;

[0022] Figure 4 yes Figure 3 Sectional view along the AA direction;

[0023] Figure 5 yes Figure 3 Cross-sectional view along the BB direction;

[0024] Figure 6 This is a schematic diagram of the plate body and cover plate in the 3D cell culture plate of this utility model.

[0025] In the picture:

[0026] 1. Plate body; 2. Reaction tank; 3. Sample feeding hole; 4. Reaction hole; 41. Top of hole; 42. Middle of hole; 43. Bottom of hole; 5. First marking group; 6. Second marking group; 7. Insertion step; 8. Cover plate. Detailed Implementation

[0027] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, not the entire structure.

[0028] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0029] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0030] In the description of this embodiment, the terms "upper," "lower," "left," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0031] like Figures 1 to 6 As shown, this embodiment provides a 3D cell culture plate, which includes a plate body 1, and a plurality of reaction grooves 2 are provided on the plate body 1, and the plurality of reaction grooves 2 are arranged in an adjacent array; each reaction groove 2 has a sample loading hole 3 and a plurality of reaction holes 4 on its bottom, and the plurality of reaction holes 4 are arranged circumferentially around the sample loading hole 3.

[0032] This allows for the aspiration and addition of culture medium through the central sample well 3. During the aspiration process, the culture medium flows into the sample well 3 for aspiration; while during the addition process, the culture medium diffuses from the sample well 3 to the surrounding reaction wells 4. This reduces interference with cells during the aspiration and addition of culture medium, improves efficiency, and enhances data stability.

[0033] In this embodiment, the plate 1 is rectangular, and several reaction tanks 2 are arranged in a rectangular array on the plate 1. The arrangement of multiple reaction tanks 2 can meet the simultaneous culture of multiple cell modules, avoiding cross-infection between them, thereby achieving high experimental efficiency and obtaining more information in a shorter time. This flexibility also allows for the creation of suitable multi-organ models based on different drugs, pathological or physiological processes, further enhancing the operability and applicability of the experiment. For example, 24 reaction tanks 2 are provided. Furthermore, each reaction tank 2 is rectangular; for example, the length and width of the reaction tank 2 are both 10mm-30mm, preferably 30mm, and the height of the reaction tank 2 is 3mm-10mm, preferably 10mm. Based on this, the reaction tank 2 can provide a large volume, greatly meeting the needs of cell culture.

[0034] like Figure 1 , Figure 2 and Figure 5As shown, in this embodiment, a plurality of reaction wells 4 surround the sample loading wells 3 circumferentially, thereby enabling the aspiration or addition of culture medium to a plurality of reaction wells 4 at one time, improving operational efficiency. Exemplarily, the plurality of reaction wells 4 are arranged in a rectangular array surrounding the sample loading wells 3. In some embodiments, each reaction tank 2 is provided with one sample loading well 3 and 16 reaction wells 4; thus, with 24 reaction tanks 2, a total of 384 reaction wells 4 are provided on the plate 1, enabling compatibility with commercial pipetting workstations and high-content imaging devices, providing a more convenient experimental platform and allowing researchers to perform experiments more easily. The system is used for operation and data collection; in other embodiments, it can be customized according to the size of the reaction tank 2; furthermore, a first mark group 5 is provided on the first side of the upper surface of the plate 1, and a second mark group 6 is provided on the second side of the upper surface of the plate 1. The first side and the second side are arranged adjacent to each other, wherein the first mark group 5 and the second mark group 6 correspond to the reaction holes 4 arrayed along the first side and the reaction holes 4 arrayed along the second side, respectively, thereby realizing the rapid positioning of the reaction holes 4; in this embodiment, one of the first mark group 5 and the second mark group 6 is a continuous set of numbers, and the other is a continuous set of letters. Furthermore, the sample well 3 is cylindrical; the above structure facilitates rapid aspiration and replacement of the culture medium; for example, the top diameter of the sample well 3 is 1.5mm-2.5mm, preferably 2.5mm; the bottom diameter of the sample well 3 is 1.5mm-2.5mm, preferably 2.5mm; the height of the sample well 3 is 1.5mm-2.5mm, preferably 2.5mm; in addition, the sample well 3 can also increase the culture medium capacity on the basis of the original volume of the reaction tank 2, further meeting the needs of cell culture.

[0035] like Figure 4 As shown, the reaction well 4 includes a top 41, a middle 42, and a bottom 43 connected in sequence; the top 41 is rectangular; the middle 42 is an inverted cone; and the bottom 43 is hemispherical. The sample loading well 3 forms a 3D microsphere structure, which is convenient for observation and also realistically simulates the cell growth environment in vivo, thus creating a foundation for providing more accurate experimental data and further facilitating applications in drug development, tissue engineering, and disease research. For example, the top 41 has a length, width, and height of 2.5 mm; the middle 42 has a height of 1 mm; and the bottom 43 has a diameter of 1 mm to 3 mm, preferably 3 mm.

[0036] like Figure 3 and Figure 6 As shown, in this embodiment, the 3D cell culture plate is also provided with a cover plate 8, and the outer periphery of the plate body 1 is provided with an insertion step 7. The cover plate 8 is inserted into the upper part of the plate body 1 to abut against the insertion step 7 for limiting, so that the reaction tank 2 can be opened or closed by the cover plate 8.

[0037] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make various obvious changes, readjustments, and substitutions without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. 3D cell culture plate, comprising a plate body (1), characterized in that, The plate (1) is provided with a plurality of reaction tanks (2), and the plurality of reaction tanks (2) are arranged in an adjacent array; Each of the reaction tanks (2) has a sample feeding hole (3) and several reaction holes (4) on its bottom wall, and the several reaction holes (4) are arranged circumferentially around the sample feeding hole (3).

2. The 3D cell culture plate of claim 1, wherein, The sample feeding hole (3) is cylindrical.

3. The 3D cell culture plate of claim 2, wherein, The top diameter of the sample feeding hole (3) is 1.5mm-2.5mm; the bottom diameter of the sample feeding hole (3) is 1.5mm-2.5mm; and the height of the sample feeding hole (3) is 1.5mm-2.5mm.

4. The 3D cell culture plate of claim 1, wherein, The reaction hole (4) includes a top (41), a middle (42), and a bottom (43) connected in sequence; the top (41) is rectangular; the middle (42) is an inverted cone; and the bottom (43) is hemispherical.

5. The 3D cell culture plate of claim 4, wherein, The length, width and height of the top part (41) of the hole are all 2.5mm; the height of the middle part (42) of the hole is 1mm; and the diameter of the bottom part (43) of the hole is 1mm-3mm.

6. The 3D cell culture plate of claim 1, wherein, The sample feeding holes (3) are arranged in a rectangular array within the reaction tank (2).

7. The 3D cell culture plate of claim 1, wherein, Several of the reaction tanks (2) are arranged in a rectangular array on the plate (1).

8. The 3D cell culture plate of claim 7, wherein, The reaction tank (2) is rectangular.

9. The 3D cell culture plate of claim 7, wherein, The length and width of the reaction tank (2) are both 10mm-30mm, and the height of the reaction tank (2) is 3mm-10mm.

10. The 3D cell culture plate according to any one of claims 1 to 9, wherein, It also includes a cover plate (8), and the outer periphery of the plate body (1) is provided with an insertion step (7). The cover plate (8) is inserted into the top of the plate body (1) so as to open or close the reaction tank (2).

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