A grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine

Abstract

This utility model discloses a simulated cultivation machine for the mycorrhizal symbiotic environment of *Pleurotus ostreatus*. The simulated cultivation machine can be either a hydroponic or soil-based symbiotic cultivation machine. It includes several symbiotic cultivation modules arranged in parallel and a supply module for supplying nutrients to each module. Each module has a support frame, an environmental simulator, and a symbiotic cultivation rack. The environmental simulator is mounted on the upper side of the support frame, simulating the light, temperature, humidity, gaseous environment, and sound wave environment for *Pleurotus ostreatus* growth. The symbiotic cultivation rack is located inside the support frame. This utility model achieves flexible control and precise simulation of the root growth state of *Pleurotus ostreatus* and *Pleurotus ostreatus* through the coordinated design of the cultivation tubes and drive mechanism within the symbiotic cultivation rack. The cultivation tubes are made of transparent material and are distributed in a gradient and connected by a circulating tube rack, providing suitable growth space and nutrient circulation channels for the plant roots.

Description

Technical Field

[0001] This utility model relates to the field of grassland white mushroom cultivation, specifically a grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine. Background Technology

[0002] *Prairia prairie* is a large fungus with high economic and ecological value. Not only is it delicious and nutritious, making it highly sought after in the food industry, but it also plays a crucial role in the ecosystem. *Prairia prairie* forms a mycorrhizal symbiotic relationship with plants such as *Leymus chinensis*, a relationship vital for maintaining the stability and health of the grassland ecosystem. Mycorrhizal fungi enhance plants' ability to absorb nutrients and water, improving their resilience, while plants provide the fungi with nutrients such as carbohydrates; the two are interdependent and mutually reinforcing. In-depth research into the mycorrhizal symbiotic mechanism of *Prairia prairie* has significant theoretical and practical value for revealing the laws governing material cycling and energy flow in grassland ecosystems, and for promoting grassland ecological restoration and sustainable development.

[0003] Traditional methods of artificially cultivating *Pleurotus ostreatus* often fail to simulate the natural symbiotic environment. On one hand, traditional cultivation containers and methods cannot precisely control environmental parameters, making it difficult to meet the specific requirements of *Pleurotus ostreatus* and *Leymus chinensis* for light, temperature, humidity, and gas conditions, resulting in unsatisfactory symbiotic effects and hindering research on mycorrhizal symbiosis. On the other hand, traditional cultivation methods make it difficult to precisely control and observe the growth status of plant roots, failing to effectively simulate the "root crossroads" in natural symbiosis. This hinders research on the spread and interaction of mycorrhizal fungi among different plant roots, limiting in-depth research into the mycorrhizal symbiotic mechanism of *Pleurotus ostreatus*.

[0004] Therefore, developing a simulated cultivation machine that can accurately simulate the mycorrhizal symbiotic environment of white mushrooms in grasslands, achieve intelligent regulation and coordinated operation of equipment, and effectively observe and study the mycorrhizal growth and symbiotic state has important practical significance and broad application prospects. Utility Model Content

[0005] The purpose of this invention is to provide a simulated cultivation machine for the mycorrhizal symbiotic environment of white mushrooms in grasslands. Through the coordinated design of the cultivation tube and the drive mechanism in the symbiotic cultivation rack, flexible control and precise simulation of the root growth status of sheepgrass and white mushrooms in grasslands are achieved.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a simulated cultivation machine for the mycorrhizal symbiotic environment of *Pleurotus ostreatus*, wherein the simulated cultivation machine is either a hydroponic or soil-based symbiotic cultivation machine. The simulated cultivation machine includes several symbiotic cultivation modules arranged in parallel and a supply module for supplying nutrients to each symbiotic cultivation module. Each symbiotic cultivation module has a support frame, an environmental simulator, and a symbiotic cultivation rack. An environmental simulator is installed on the upper side of the support frame, which simulates the light, temperature, humidity, gas environment, and sound wave environment for the growth of *Pleurotus ostreatus*. A symbiotic cultivation rack is installed inside the support frame. The symbiotic cultivation rack includes a cultivation rack body and a nutrient supply box arranged in an upper and lower configuration. The cultivation rack body includes multiple cultivation tubes arranged in a gradient distribution. The first and last ends of the multiple cultivation tubes are connected through a circulating tube frame. Each cultivation tube has a planting opening, and *Pleurotus ostreatus* and *Pleurotus ostreatus* are spaced apart inside the planting opening. A number of partitions corresponding to the number of planting openings are rotatably installed inside each cultivation tube.

[0007] Preferably, the nutrient supply box is equipped with a pump, and the output side of the pump is connected to the circulation tube rack at the top of the cultivation tubes via a supply pipe. The nutrient solution, aided by gravity and the optimized layout of the circulation tube rack, flows evenly from top to bottom through each cultivation tube, ensuring that each *Pleurotus ostreatus* and *Leymus chinensis* plant receives sufficient and balanced nutrient nourishment.

[0008] Preferably, the cultivation tube is made of a transparent material, and the partition is circular and fits the cultivation tube. The partition has flow holes at its bottom, and the cultivation tube is equipped with a drive mechanism to regulate the movement of the partition. Researchers can directly observe the growth of the roots of Leymus chinensis and Trichoderma pentaphyllum, the formation process of mycorrhizae, and the interaction between them through the cultivation tube without disrupting the cultivation environment. This achieves real-time, non-destructive monitoring of the symbiotic state, providing an intuitive and accurate observation window for in-depth research on mycorrhizal symbiosis mechanisms.

[0009] Preferably, the drive mechanism includes a rocker arm servo and a linkage frame. The rocker arm shaft of the rocker arm servo is connected to the linkage frame, which has multiple bushings corresponding to the number and position of the partitions. Each bushing contains a shaft connected to the shaft end of the partition. The partitions rotate relative to the cultivation tube as the rocker arm servo moves. The flow holes at the bottom of the partitions ensure a certain degree of sealing of the internal space of the cultivation tube while allowing nutrient solution and gas to pass through, simulating the water and gas exchange conditions around the roots at different growth stages in the natural environment. The drive mechanism installed on the cultivation tube can precisely control the movement of the partitions, flexibly changing the degree of openness inside the cultivation tube according to the different growth stages of *Leymus chinensis* and *Pleurotus ostreatus*.

[0010] Preferably, the front side of the support frame is equipped with a host unit that is connected to the environmental simulator, pump body and drive mechanism. The host unit is based on a multimodal perception, decision-making and execution closed-loop architecture to realize intelligent control of cultivation environment parameters and coordinated operation of equipment.

[0011] Preferably, the lower side of the main unit is equipped with an image acquisition device for detecting the mycorrhizal growth status of *Pleurotus ostreatus*. The image acquisition device can acquire high-definition images of the mycorrhizae in real time and non-destructively.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] 1. This invention achieves flexible control and precise simulation of the root growth status of *Leymus chinensis* and *Trichoderma pentaphyllum* through the coordinated design of the cultivation tubes and drive mechanism in a symbiotic cultivation rack. The cultivation tubes are made of transparent material and are distributed in a gradient and connected by a circulating tube rack, providing suitable growth space and nutrient circulation channels for the plant roots. The pendulum servo motor in the drive mechanism works in conjunction with the linkage frame to precisely control the movement of the partitions inside each cultivation tube. In the early stage of growth of *Leymus chinensis* and *Trichoderma pentaphyllum*, the partitions seal the inside of the cultivation tubes, allowing only the nutrient solution to pass through the flow holes, simulating a specific early growth environment; in the middle stage of growth, the partitions open the cultivation tubes, allowing the roots of the two plants to intertwine freely, precisely simulating the "root crossover zone" in natural symbiosis. This coordinated design provides ideal experimental conditions for studying the dissemination efficiency of mycorrhizal fungi in heterogeneous root systems.

[0014] 2. This invention achieves precise simulation and intelligent dynamic control of the mycorrhizal symbiotic environment of *Pleurotus ostreatus* through the collaborative design of an environmental simulator and a host computer. The environmental simulator comprehensively simulates the natural symbiotic conditions under the combined effects of multiple factors such as light, temperature, humidity, gas environment, and sound wave environment, providing the host computer with a rich foundation of environmental parameters. Based on a multimodal perception, decision-making, and execution closed-loop architecture, the host computer receives various data feedback from the environmental simulator in real time and makes intelligent decisions in conjunction with a preset symbiotic growth model. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the symbiotic cultivation module in an embodiment of the present invention;

[0017] Figure 3 This is a schematic diagram of the symbiotic cultivation rack in an embodiment of the present invention;

[0018] Figure 4 This is a schematic diagram of the drive mechanism and partition in an embodiment of the present invention.

[0019] In the diagram: 1. Support frame; 2. Environmental simulator; 3. Cultivation frame; 31. Cultivation pipe; 32. Circulation pipe rack; 33. Planting port; 34. Partition; 35. Flow hole; 36. Swing servo motor; 37. Linkage frame; 38. Bushing; 4. Nutrient supply box; 5. Main unit; 6. Image acquisition device. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] In the description of this utility model, it should be noted that the terms "vertical", "up", "down", "horizontal", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0022] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0023] Please see Figure 1 This utility model provides a technical solution: a grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine, wherein the symbiotic environment simulation cultivation machine is a hydroponic symbiotic cultivation machine, and the symbiotic environment simulation cultivation machine includes several symbiotic cultivation modules arranged in parallel and a supply module for supplying nutrients to each symbiotic cultivation module.

[0024] Please see Figure 2 , Figure 3In this embodiment, each symbiotic cultivation module has a support frame 1, an environment simulator 2, and a symbiotic cultivation rack. The environment simulator 2 is installed on the upper side of the support frame 1. The environment simulator 2 is used to simulate the light, temperature, humidity, gas environment, and sound wave environment for the growth of white shiitake mushrooms. The symbiotic cultivation rack is set inside the support frame 1. The symbiotic cultivation rack includes a cultivation rack body 3 distributed vertically and a nutrient supply box 4. The cultivation rack body 3 includes a plurality of cultivation tubes 31 distributed in a gradient. The first and last ends of the plurality of cultivation tubes 31 are connected through a circulation tube rack 32. Each cultivation tube 31 is provided with a planting port 33. Sheep grass and white shiitake mushrooms are placed at intervals inside the planting port 33. Each cultivation tube 31 is rotatably installed with partitions 34 corresponding to the number of planting ports 33.

[0025] In this embodiment, a pump body is installed inside the nutrient supply box 4. The output side of the pump body is connected to the circulation tube rack 32 at the top of the cultivation tube 31 through a supply pipe. The nutrient solution can flow evenly from top to bottom through each cultivation tube 31 with the help of gravity and the reasonable layout of the circulation tube rack 32, so that each grassland white mushroom and sheep grass can receive sufficient and balanced nutrient nourishment.

[0026] Please see Figure 3 , Figure 4 In this embodiment, the cultivation tube 31 is made of transparent material, and the partition 34 is circular and fits the cultivation tube 31. The bottom of the partition 34 has a flow hole 35, and the cultivation tube 31 is equipped with a drive mechanism for controlling the movement of the partition 34. Researchers can directly observe the growth of the roots of *Leymus chinensis* and *Trichoderma pentaphyllum*, the formation process of mycorrhizae, and the interaction between them through the cultivation tube 31 without damaging the cultivation environment. This achieves real-time, non-destructive monitoring of the symbiotic state, providing an intuitive and accurate observation window for in-depth research on the mycorrhizal symbiosis mechanism. The drive mechanism includes a swing arm servo motor 36 and a linkage frame 37. The swing arm shaft of the swing arm servo motor 36 is connected to the linkage frame 37. The linkage frame 37 has multiple bushings 38 corresponding to the number and position of the partitions 34. Each bushing 38 contains a shaft connected to the shaft end of the partition 34. The partitions 34 move with the swing arm servo motor 36 and rotate relative to the cultivation tube 31. The flow holes 35 at the bottom of the partition 34 ensure that the partition 34 has a certain sealing effect on the internal space of the cultivation tube 31, while allowing nutrient solution and gas to pass through, simulating the water and gas exchange conditions around the roots at different growth stages in the natural environment. The drive mechanism installed on the cultivation tube 31 can precisely control the movement of the partition 34, flexibly changing the degree of openness inside the cultivation tube 31 according to the different growth stages of Leymus chinensis and Pleurotus ostreatus. In the early stage of growth, the drive mechanism causes the partition 34 to seal the cultivation tube 31, creating a relatively independent microenvironment for growth; in the middle stage of growth, the drive mechanism opens the partition 34, allowing the roots of the two plants to intertwine freely, accurately simulating the "root crossroads" in natural symbiosis, providing ideal experimental conditions for studying the dispersal efficiency of mycorrhizal fungi in heterogeneous root systems.

[0027] Please see Figure 2 In this embodiment, a host 5 connected to the environmental simulator 2, pump body, and drive mechanism is configured on the front side of the support frame 1. The host 5 is based on a multimodal perception, decision-making, and execution closed-loop architecture to realize intelligent control of cultivation environment parameters and coordinated operation of equipment. An image acquisition device 6 for detecting the mycorrhizal growth status of *Trichoderma pentaphyllum* is located on the lower side of the host 5. The image acquisition device 6 can acquire high-definition images of the mycorrhizae in real time and non-destructively. Through high-resolution imaging technology, it can clearly present the morphology, structure, color, and symbiotic details of the mycorrhizae with the *Leymus chinensis* root system, such as the mycelial entanglement method and the degree of mycorrhizal infection. This detailed image information provides researchers with rich and accurate data sources, enabling them to more accurately analyze the changes in mycorrhizae at different growth stages and to conduct in-depth research on the interaction mechanism between mycorrhizal fungi and plants, thereby greatly improving the scientific rigor and reliability of the research.

[0028] The embodiments of this utility model are given for the purpose of illustration and description. Although the embodiments of this utility model have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the utility model. Any changes, modifications, substitutions and variations made by those skilled in the art to the above embodiments within the scope of this utility model should be included within the protection scope of this utility model.

Claims

1. A grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine, characterized in that, The symbiotic environment simulation cultivation machine is either a hydroponic symbiotic cultivation machine or a soil-based symbiotic cultivation machine. The symbiotic environment simulation cultivation machine includes several symbiotic cultivation modules arranged in parallel and a supply module for supplying nutrients to each symbiotic cultivation module. Each symbiotic cultivation module has a support frame (1), an environment simulator (2), and a symbiotic cultivation rack. The environment simulator (2) is installed on the upper side of the support frame (1), and the symbiotic cultivation rack is installed on the inner side of the support frame (1). The symbiotic cultivation rack includes... The cultivation frame (3) and nutrient supply box (4) are distributed in an upper and lower position. The cultivation frame (3) includes multiple cultivation tubes (31) distributed in a gradient. The first and last ends of the multiple cultivation tubes (31) are connected through a circulating tube frame (32). Each cultivation tube (31) is provided with a planting port (33). Sheep grass and grassland white mushroom are placed at intervals in the planting port (33). Each cultivation tube (31) is rotatably installed with partitions (34) corresponding to the number of planting ports (33).

2. The grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine according to claim 1, characterized in that: The nutrient supply box (4) is equipped with a pump body, and the output side of the pump body is connected to the circulation pipe rack (32) at the top of the cultivation pipe (31) through the supply pipe.

3. The grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine according to claim 1, characterized in that: The cultivation tube (31) is made of transparent material, the partition (34) is circular and fits the cultivation tube (31), the bottom of the partition (34) is provided with a flow hole (35), and the cultivation tube (31) is equipped with a drive mechanism for regulating the movement of the partition (34).

4. The grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine according to claim 3, characterized in that: The drive mechanism includes a rocker arm servo (36) and a linkage frame (37). The rocker arm shaft of the rocker arm servo (36) is connected to the linkage frame (37). The linkage frame (37) is provided with multiple bushings (38) corresponding to the number and position of the partitions (34). The bushings (38) are provided with shafts connected to the shaft ends of the partitions (34). The partitions (34) move with the rocker arm servo (36) and rotate relative to the cultivation tube (31).

5. The grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine according to claim 1, characterized in that: The front side of the support frame (1) is equipped with a host (5) that is connected to the environmental simulator (2), the pump body and the drive mechanism.

6. The grassland white mushroom mycorrhizal symbiotic environment simulation cultivation machine according to claim 5, characterized in that: The host (5) is equipped with an image acquisition device (6) on its lower side for detecting the mycorrhizal growth status of white mushrooms.

Images