A tissue filtration device

Abstract

This utility model discloses a tissue filtration device, including a centrifuge tube, a first filter element, a second filter element, and a propulsion component. The centrifuge tube has an inner cavity with an opening. The first filter element is installed in the inner cavity and has a first filter chamber. The first filter chamber has a first filter hole and an opening, which are connected to the first filter chamber. Tissue homogenate can pass through the first filter hole, while connective tissue is blocked by the first filter element and remains in the first filter chamber. The second filter element is installed at the bottom of the first filter chamber and has a second filter chamber. The second filter chamber has a second filter hole and an opening, the diameter of which is smaller than that of the first filter hole. The second filter hole and the opening are connected to the second filter chamber, where connective tissue is collected. The propulsion component is located in the first filter chamber and is used to propel the tissue homogenate towards the second filter element. The propulsion component applies pressure to the tissue homogenate. This utility model can reduce the residual rate of connective tissue in tissue homogenate, shorten processing time, and improve filtration efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical technology, specifically relating to a tissue filtration device. Background Technology

[0002] In biomedical research, tissue digestion or tissue disruption is a key pretreatment step in many experiments, with the aim of releasing cells, nucleic acids, proteins, or other target molecules.

[0003] In biomedical experiments (such as single-cell sequencing, primary cell culture, protein extraction, etc.), tissue samples often need to be mechanically broken or enzymatically digested into homogenates, and then further filtered to remove impurities such as connective tissue.

[0004] Existing technology uses a single-layer filter cartridge, which can only intercept large particles of connective tissue, while fine fibers can easily pass through the filter cartridge, resulting in low filtration efficiency. Utility Model Content

[0005] This invention provides a tissue filtration device that solves the technical problem of fine fibers easily passing through the filter cartridge and resulting in low filtration efficiency.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0007] A tissue filtration device, comprising:

[0008] Centrifuge tube, the centrifuge tube has an inner cavity, and the inner cavity has a tube opening;

[0009] Its characteristic is that it further includes:

[0010] Filter element one is installed in the inner cavity. Filter element one has a filter chamber one. Filter chamber one has a filter hole one and an opening one. Filter hole one and opening one are connected to filter chamber one. Tissue homogenate can pass through filter hole one. Connective tissue in tissue homogenate is blocked by filter element one and remains in filter chamber one.

[0011] Filter element two is installed at the bottom of filter chamber one. Filter element two has filter chamber two, filter chamber two has filter hole two and opening two. The diameter of filter hole two is smaller than the diameter of filter hole one. Filter hole two and opening two are connected to filter chamber two. Connective tissue is collected in filter chamber two.

[0012] The propulsion component is located in the first filter chamber and is used to propel the tissue homogenate towards the second filter chamber. The propulsion component applies pressure to the tissue homogenate.

[0013] Furthermore, the filter element 2 is provided with a connecting post, the propulsion element is provided with a guide post, and the guide post and the propulsion element are provided with a through hole that runs vertically through the top and bottom. When the propulsion element is in the propulsion state, the connecting post passes through the through hole.

[0014] Furthermore, the top of the connecting column is provided with an end, and the cross-sectional area of ​​the end gradually decreases from bottom to top.

[0015] Furthermore, the top of the guide post is sloped to form a pointed tip.

[0016] Furthermore, the propulsion component is made of rubber.

[0017] Furthermore, a reinforcing rib is provided at the connection between the side and bottom of the filter element 2, and a reinforcing rib 2 is provided between the bottom of the filter element 2 and the connecting column. The reinforcing rib 2 and the reinforcing rib 1 are fixedly connected.

[0018] Furthermore, the centrifuge tube has an external thread on its outer side, which is used to connect a sealing cap or a limiting head. When filtering tissue homogenate, the limiting head is threaded onto the external thread, and the filter element is limited by the limiting head; when the centrifuge tube is not in use, the sealing cap is threaded onto the external thread for sealing the tube opening.

[0019] Furthermore, the limiting head is provided with a limiting hole, the filter element is provided with an end plate, the end plate is provided with a limiting post, and the limiting post is embedded into the limiting hole from top to bottom.

[0020] Furthermore, a protrusion is provided on the top edge of the end plate, and a groove is formed between the protrusion and the end plate.

[0021] Furthermore, a stiffening plate is fixed between the protrusion and the end plate.

[0022] This utility model, by adopting the above-mentioned technical solution, has the following beneficial effects:

[0023] (1) This utility model uses filter element one to initially intercept most of the connective tissue and filter element two to retain small fibers in a second stage, thereby achieving gradient separation of connective tissue and reducing the residual rate of connective tissue in tissue homogenate.

[0024] (2) The present invention uses a propulsion component to apply pressure to the tissue homogenate, thereby accelerating the passage of the tissue homogenate through filter component one and filter component two, shortening the processing time and improving the filtration efficiency. Attached Figure Description

[0025] The present invention will be further described below with reference to the accompanying drawings:

[0026] Figure 1 This is a schematic diagram of the connection between the sealing cap and the centrifuge tube in this utility model;

[0027] Figure 2 This is a schematic diagram of the centrifuge tube in this utility model;

[0028] Figure 3 This is a schematic diagram of the connection between the centrifuge tube and the limiting head in this utility model;

[0029] Figure 4 This is a schematic diagram of the limiting head in this utility model;

[0030] Figure 5 This is a schematic diagram of the structure of filter element one in this utility model;

[0031] Figure 6 for Figure 5 The main view;

[0032] Figure 7 This is a schematic diagram of the structure of filter element one and filter element two after installation in this utility model;

[0033] Figure 8 This is a schematic diagram of the structure of filter element two in this utility model;

[0034] Figure 9 This is a schematic diagram of the structure of the propulsion component after installation in this utility model;

[0035] Figure 10 This is a schematic diagram of the propulsion component in this utility model;

[0036] Figure 11 This is a cross-sectional view of the propulsion component in this utility model.

[0037] In the diagram, 1-centrifuge tube; 2-sealing cap; 3-external thread; 4-limiting head; 5-limiting hole; 6-anti-slip protrusion; 7-filter element one; 8-filter chamber one; 9-opening one; 10-filter hole one; 11-end plate; 12-protrusion; 13-reinforcing plate; 14-limiting post; 15-filter element two; 16-filter hole two; 17-filter chamber two; 18-opening two; 19-connecting post; 20-end; 21-reinforcing rib one; 22-reinforcing rib two; 23-propeller; 24-guide post; 25-sloping surface; 26-pointed tip; 27-through hole; 28-inner cavity; 29-pipe opening. Detailed Implementation

[0038] like Figures 1 to 11 As shown, this utility model discloses a tissue filtration device, which includes a first filter element 7, a second filter element 15, and a propulsion element 23. The tissue filtration device filters the tissue homogenate formed after the tissue is digested or broken up in the centrifuge tube 1.

[0039] like Figure 1 and Figure 2 As shown, this utility model uses a 50ml centrifuge tube 1. The centrifuge tube 1 has an inner cavity 28 and a tube opening 29. The outer side of the centrifuge tube 1 has an external thread 3. When the centrifuge tube 1 is not in use, a sealing cap 2 is threaded onto the external thread 3. The sealing cap 2 seals the tube opening 29 to prevent impurities from entering the inner cavity 28.

[0040] When filtering the tissue homogenate formed after tissue digestion or disruption, remove the sealing cap 2 and thread a limiting head 4 through the external thread 3. The limiting head 4 has a limiting hole 5 and an anti-slip protrusion 6 on its outer side to facilitate unscrewing the limiting head 4. Figure 3 As shown.

[0041] like Figure 5 and Figure 6 As shown, filter element 7 is a filter barrel. Filter element 7 has a filter chamber 8. Filter holes 10 are provided on the sides and bottom of filter element 7, and an opening 9 is provided on the top of filter element 7. The filter holes 10 and the opening 9 communicate with the filter chamber 8. An end plate 11 is provided on the top of filter element 7, and the end plate 11 is correspondingly arranged with the opening 9. The end plate 11 is provided with a limiting post 14. A protrusion 12 is provided on the top edge of the end plate 11, and a groove is formed between the protrusion 12 and the end plate 11. The protrusion 12 can block the tissue homogenate, allowing the tissue homogenate to enter the filter chamber 8 through the opening 9. A stiffening plate 13 is fixed between the protrusion 12 and the end plate 11 to increase the connection strength between the protrusion 12 and the end plate 11. When installing filter element 7, filter element 7 is installed in the inner cavity 28 of centrifuge tube 1. At the same time, the limiting column 14 is embedded into the limiting hole 5 from top to bottom, and filter element 7 is limited on the limiting head 4, which improves the stability of tissue homogenization filtration.

[0042] like Figure 8 As shown, filter element 2 15 uses a filter screen. Filter element 2 15 has a filter chamber 2 17. Filter holes 2 16 are provided on the side and bottom of filter element 2 15, and an opening 2 18 is provided on the top of filter element 2 15. The size of filter hole 2 16 is smaller than that of filter hole 10. Filter hole 2 16 and opening 2 18 connect to filter chamber 2 17. Filter element 2 15 has a connecting post 19. The top of the connecting post 19 has an end 20, and the cross-sectional area of ​​the end 20 gradually decreases from bottom to top. Reinforcing rib 1 21 is provided at the connection between the side and bottom of filter element 2 15. Reinforcing rib 22 is provided between the bottom of filter element 2 15 and the connecting post 19. Reinforcing rib 22 and reinforcing rib 1 21 are fixedly connected to increase the strength of filter element 2 15 and meet the installation and use requirements of the subsequent propulsion component 23. After filter element 1 7 is installed, force is applied to the connecting post 19 to install filter element 2 15 in the bottom of filter chamber 1 8, as shown. Figure 7 As shown.

[0043] After filter element 2 (15) is installed, the tissue homogenate formed after tissue digestion or disruption is slowly transferred to filter element 1 (7). The tissue homogenate can pass through filter holes 10, while most of the connective tissue in the tissue homogenate is blocked by filter element 1 (7) and remains in filter chamber 8. Filter element 2 (15) is used for secondary retention of small fibers, achieving gradient separation of connective tissue and significantly reducing the residual connective tissue rate in the tissue homogenate.

[0044] like Figure 10 and Figure 11 As shown, the propeller 23 is made of rubber and has a guide post 24. The guide post 24 and the propeller 23 form a through hole 27. The top of the guide post 24 has a slope 25, forming a pointed tip 26. When the tissue homogenate cannot be filtered normally, the propeller 23 is used for propulsion. The propeller 23 is placed in the first filter chamber 8, and the propeller 23 is attached to the inner wall of the first filter 7. The connecting post 19 is inserted into the through hole 27, and the propeller 23 is sleeved on the connecting post 19 of the second filter 15. The connecting post 19 is attached to the guide post 24, as shown. Figure 9 As shown, the propeller 23 is continuously pushed towards the filter element 15. The propeller 23 applies pressure to the tissue homogenate, accelerating the passage of the tissue homogenate through the filter element 7 and the filter element 15, shortening the processing time and improving the filtration efficiency.

[0045] After filtration, force is applied to the tip 26 of the guide column 24 to remove the pusher 23. Then, force is applied to the connecting column 19 to remove the second filter element 15. Next, force is applied to the protrusion 12 of the first filter element 7 to remove the first filter element 7, removing the connective tissue. The centrifuge tubes 1 containing the tissue homogenate are then collected and placed for subsequent experiments. The first filter element 7, the second filter element 15, and the pusher 23 are then cleaned and sterilized to ensure their reusability.

[0046] The above are merely specific embodiments of this utility model, but the technical features of this utility model are not limited thereto. Any simple changes, equivalent substitutions, or modifications made based on this utility model to solve essentially the same technical problems and achieve essentially the same technical effects are all covered within the protection scope of this utility model.

Claims

1. A tissue filtration device, comprising: Centrifuge tube, wherein the centrifuge tube has an inner cavity and an opening in the inner cavity; Its characteristic is that it further includes: A filter element 1 is installed in the inner cavity. The filter element 1 has a filter chamber 1, and the filter chamber 1 has a filter hole 1 and an opening 1. The filter hole 1 and the opening 1 are connected to the filter chamber 1. The tissue homogenate can pass through the filter hole 1, and the connective tissue in the tissue homogenate is blocked by the filter element 1 and remains in the filter chamber 1. A second filter element is installed in the bottom of the first filter chamber. The second filter element has a second filter chamber, which has a second filter hole and a second opening. The diameter of the second filter hole is smaller than the diameter of the first filter hole. The second filter hole and the second opening communicate with the second filter chamber. Connective tissue is collected in the second filter chamber; A propulsion component is disposed in the first filter chamber and is used to propel the tissue homogenate toward the second filter chamber. The propulsion component applies pressure to the tissue homogenate.

2. The tissue filtration device according to claim 1, characterized in that: The filter element 2 is provided with a connecting post, the propulsion element is provided with a guide post, and the guide post and the propulsion element are provided with a through hole that runs vertically through each other. When the propulsion element is in the propulsion state, the connecting post passes through the through hole.

3. The tissue filtration device according to claim 2, characterized in that: The top of the connecting column is provided with an end, and the cross-sectional area of ​​the end gradually decreases from bottom to top.

4. A tissue filtration device according to claim 2, characterized in that: The top of the guide post has a slope, forming a pointed tip.

5. A tissue filtration device according to claim 2, characterized in that: The propulsion component is made of rubber.

6. A tissue filtration device according to claim 2, characterized in that: The side and bottom of the filter element 2 are provided with a reinforcing rib 1, and the bottom of the filter element 2 and the connecting post are provided with a reinforcing rib 2. The reinforcing rib 2 and the reinforcing rib 1 are fixedly connected.

7. A tissue filtration device according to claim 1, characterized in that: The centrifuge tube is provided with external threads on the outside, which are used for threaded connection of sealing caps or limiting heads; When filtering tissue homogenate, the limiting head is threadedly connected to the external thread, and the filter element is limited on the limiting head; When the centrifuge tube is not in use, the sealing cap is threaded onto the external thread for sealing the tube opening.

8. A tissue filtration device according to claim 7, characterized in that: The limiting head is provided with a limiting hole, the filter element is provided with an end plate, the end plate is provided with a limiting post, and the limiting post is embedded into the limiting hole from top to bottom.

9. A tissue filtration device according to claim 8, characterized in that: The top edge of the end plate is provided with a protrusion, and a groove is formed between the protrusion and the end plate.

10. A tissue filtration device according to claim 9, characterized in that: A stiffening plate is fixed between the protrusion and the end plate.

Images