A cell culture flask to prevent contamination

Abstract

This utility model discloses a cell culture flask for preventing contamination, relating to the field of cell culture flask technology. It includes a mother flask and a daughter flask, each with an installation tube fixedly connected to one side. A connecting tube is provided between the two installation tubes, and a clamping assembly is provided on one side of each installation tube. The connecting tube is fixedly connected to the installation tube via the clamping assembly. This utility model employs the above structure, installing a baffle at the sealing cap. A HEPA filter and a filter screen are installed inside the baffle. The filter screen performs preliminary filtration of large particles in the air, and the HEPA filter efficiently filters out micron-sized particles, including most microorganisms. This significantly reduces the risk of microorganisms entering the culture flask and contaminating the cells, ensuring a sterile environment for cell culture, reducing cell death or mutation due to contamination, thereby improving the purity of the gas entering the flask and preventing harmful gases or impurities from affecting cell culture.

Description

Technical Field

[0001] This invention belongs to the field of cell culture flask technology, and specifically relates to a cell culture flask that prevents contamination. Background Technology

[0002] Cell culture flasks are usually made of polystyrene or glass. Polystyrene culture flasks have good transparency, making it easy to observe cell growth. The surface is also specially treated to promote cell adhesion and growth. Cell culture flasks usually need to be equipped with anti-contamination structures to prevent cell contamination.

[0003] For example, CN221544653U discloses a type of anti-contamination mother cell culture flask, which includes a mother cell culture flask and a daughter cell culture flask. The top of the mother cell culture flask and the daughter cell culture flask are provided with a stopper, and the upper part of the stopper is provided with a dust cover. The dust cover is provided with vent holes evenly distributed. The stopper can effectively prevent contaminants in the external environment from entering the interior of the daughter cell culture flask, ensuring that the cell culture fluid inside the daughter cell culture flask can be cultured normally. A connecting tube is provided between the mother cell culture flask and the daughter cell culture flask, and a cell filter A is provided inside the connecting tube.

[0004] The aforementioned patent can prevent contamination of the cell culture medium in the mother bottle, thus ensuring that the cell culture medium in the mother bottle can still be used for normal culture experiments after sampling and analysis. However, the device still has some defects that need to be improved. There are a large number of microorganisms in the air, such as bacteria, fungi, and viruses. Even if the bottle stopper and dust cover have a certain filtering effect, it is difficult to completely block them, which may still cause cell contamination. In addition, after the culture work is completed, the connecting tube between the daughter bottle and the mother bottle is not detachable, which makes subsequent cleaning and maintenance inconvenient. Utility Model Content

[0005] In view of the problems mentioned in the background art, the purpose of this utility model is to provide a cell culture flask that prevents contamination, so as to solve the problems mentioned in the background art.

[0006] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0007] A cell culture flask for preventing contamination includes a mother flask and a daughter flask. An installation tube is fixedly connected to one side of each of the mother and daughter flasks. A connecting tube is provided between the two installation tubes. A clamping assembly is provided on one side of each installation tube. The connecting tube is fixedly connected to the installation tube via the clamping assembly. A screw head is fixedly connected to the top of each of the mother and daughter flasks. A sealing cap is threaded onto the upper side of the screw head. A filter mechanism is provided on one side of the sealing cap. A stopper is inserted into the inside of each of the mother and daughter flasks, and a vent is provided inside the stopper.

[0008] As a preferred technical solution, the filtration mechanism includes a cover, which is fitted and connected to the upper side of the sealing cover. A filter screen is fixedly connected to the inner side of the cover, and a HEPA filter is fixedly connected to the lower side of the filter screen inside the cover. An installation assembly is provided between the cover and the sealing cover.

[0009] As a preferred technical solution, the mounting component includes a plug, which is fixedly connected to both sides of the cover. Mounting frames are fixedly connected to both sides of the sealing cover. A slot is provided inside the mounting frame, and the plug is inserted into the slot. A locking hole is provided inside the plug, and a locking block is inserted into the locking hole. A spring is fixedly connected inside the mounting frame, and a slider is fixedly connected to one side of the spring. One side of the slider is fixedly connected to one side of the locking block.

[0010] As a preferred technical solution, limit grooves are provided on both sides of the inner side of the mounting frame, and one side of the slider is slidably connected to the limit groove.

[0011] As a preferred technical solution, the pipe clamping assembly includes a fixing frame, which is fixedly connected to one side of the installation pipe. The fixing frame has a sliding groove inside, and a moving block is slidably connected inside the sliding groove. A clamping block is fixedly connected to one side of the moving block. A lead screw is rotatably connected inside the sliding groove, and a torsion block is fixedly connected to the end of the lead screw. One side of the moving block is threadedly connected to the lead screw.

[0012] In summary, the present invention has the following main advantages:

[0013] First, this utility model installs a baffle at the sealing cap, and a HEPA filter and a filter screen are installed inside the baffle. The filter screen is used to initially filter large particles in the air, and then the HEPA filter can efficiently filter out micron-sized particles, including most microorganisms, which greatly reduces the risk of microorganisms entering the culture bottle and contaminating the cells, ensuring a sterile environment for cell culture, reducing the possibility of cell death or mutation due to contamination, thereby improving the purity of the gas entering the bottle and preventing harmful gases or impurities from affecting cell culture.

[0014] Secondly, this utility model has an installation tube fixed on both the mother bottle and the daughter bottle, and the two installation tubes are connected by a clamping assembly to form a connecting tube. Therefore, the connecting tube can be removed and replaced, which facilitates the cleaning of the connecting tube, the mother bottle, and the daughter bottle. After the culture work is completed, it can be easily cleaned to remove residual cell culture medium and metabolic products, avoid bacterial growth, and improve the cleaning and maintenance efficiency of cell culture flasks. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the installation component structure of this utility model;

[0017] Figure 3 This is a utility model Figure 2 A magnified structural diagram at point A;

[0018] Figure 4 This is a schematic diagram of the internal structure of the cover of this utility model;

[0019] Figure 5 This is a schematic diagram of the clamping assembly structure of this utility model.

[0020] Reference numerals: 1. Mother bottle; 2. Daughter bottle; 3. Mounting tube; 4. Connecting tube; 5. Screw head; 6. Sealing cap; 7. Bottle stopper; 8. Vent hole; 9. Filtering mechanism; 91. Cover; 92. Filter screen; 93. HEPA filter; 94. Mounting assembly; 941. Insert block; 942. Locking hole; 943. Slot; 944. Locking block; 945. Slider; 946. Spring; 947. Mounting frame; 948. Limiting groove; 10. Tube clamping assembly; 101. Fixing bracket; 102. Lead screw; 103. Torsion block; 104. Slide groove; 105. Moving block; 106. Clamping block. Detailed Implementation

[0021] Example

[0022] refer to Figures 1 to 5 This embodiment describes a cell culture flask for preventing contamination, comprising a mother flask 1 and a daughter flask 2. Each of the mother flask 1 and daughter flask 2 is fixedly connected to one side of an installation tube 3. A connecting tube 4 is provided between the two installation tubes 3, and a sealing ring is provided between the installation tube 3 and the connecting tube 4. The connecting tube 4 ensures that the culture environments of the cell culture mother flask 1 and the cell culture daughter flask 2 are completely identical. When sampling and analysis of the cultured cells is required, sampling and analysis can be performed in the cell culture daughter flask 2, preventing contamination of the cell culture mother flask. The cell fluid in bottle 1 causes contamination. A clamping assembly 10 is provided on one side of the installation tube 3. The connecting tube 4 is fixedly connected to the installation tube 3 through the clamping assembly 10. A screw head 5 is fixedly connected to the top of both the mother bottle 1 and the daughter bottle 2. A sealing cap 6 is threadedly connected to the upper side of the screw head 5. A filter mechanism 9 is provided on one side of the sealing cap 6. A bottle stopper 7 is inserted into the inside of both the mother bottle 1 and the daughter bottle 2. A ventilation hole 8 is opened inside the bottle stopper 7 to ensure normal airflow into and out of the mother bottle 1 or the daughter bottle 2.

[0023] refer to Figure 2 and Figure 4The filtration mechanism 9 includes a cover 91, which is fitted to the upper side of the sealing cover 6. A filter screen 92 is fixedly connected to the inside of the cover 91, and a HEPA filter 93 is fixedly connected to the inside of the cover 91 below the filter screen 92. An installation component 94 is provided between the cover 91 and the sealing cover 6. By setting the filtration mechanism 9, the cover 91 is fitted to the top of the sealing cover 6 and fixed by the installation component 94. When air enters the bottle, large particles in the air are first initially filtered by the filter screen 92. Then, the air passes through the HEPA filter to filter out micron-sized particles, including most microorganisms. The filter element of the HEPA filter is usually made of materials such as glass fiber, synthetic fiber, or paper. These materials have a very fine fiber structure, which can form complex pore channels, thereby achieving a highly efficient filtration effect.

[0024] refer to Figure 3 The mounting component 94 includes a plug 941, which is fixedly connected to both sides of the cover 91. Mounting frames 947 are fixedly connected to both sides of the sealing cover 6. A slot 943 is provided inside the mounting frame 947, and the plug 941 is inserted into the slot 943. A locking hole 942 is provided inside the plug 941, and a locking block 944 is inserted into the locking hole 942. A spring 946 is fixedly connected inside the mounting frame 947, and a slider 945 is fixedly connected to one side of the spring 946. One side of the slider 945 is fixedly connected to one side of the locking block 944. By setting up the mounting component 94, during installation, the plug 941 on the cover 91 is aligned with the mounting frame 947 on the sealing cover 6 and inserted. At this time, the locking block 944, under the action of the spring 946, is inserted into the locking hole 942 of the plug 941, completing the fixed connection between the cover 91 and the sealing cover 6. Disassembly is also very convenient; simply lift the cover 91 upwards.

[0025] refer to Figure 3 The mounting frame 947 has limit grooves 948 on both sides inside. One side of the slider 945 is slidably connected to the limit groove 948. By setting the limit groove 948, the slider 945 slides along the limit groove 948. The limit groove 948 restricts the movement direction of the slider 945, ensuring that the card block 944 can be accurately inserted into and removed from the card hole 942.

[0026] refer to Figure 5The clamping assembly 10 includes a fixing frame 101, which is fixedly connected to one side of the mounting tube 3. The fixing frame 101 has a sliding groove 104 inside, and a moving block 105 is slidably connected inside the sliding groove 104. A clamping block 106 is fixedly connected to one side of the moving block 105. A lead screw 102 is rotatably connected inside the sliding groove 104, and a torsion block 103 is fixedly connected to the end of the lead screw 102. One side of the moving block 105 is threadedly connected to the lead screw 102. By setting the clamping assembly 10, rotating the torsion block 103 drives the lead screw 102 to rotate, causing the moving block 105 to move within the sliding groove 104. The moving block 105 drives the clamping block 106 to move, thereby clamping or loosening the connecting tube 4. After the culture work is completed, the connecting tube 4 can be removed for easy cleaning of the connecting tube 4, mother bottle 1, and daughter bottle 2.

[0027] Operating principle and advantages: Place the mother bottle 1 and daughter bottle 2 side by side, ensuring that the installation tubes 3 on one side of both correspond. Place the connecting tube 4 between the installation tubes 3 of the mother bottle 1 and daughter bottle 2. Rotate the torsion block 103 to drive the lead screw 102 to rotate. The rotation of the lead screw 102 will cause the moving block 105 to move within the slide groove 104. The moving block 105 will drive the clamping block 106 to move until the clamping block 106 clamps the connecting tube 4, making the connecting tube 4 stably connected to the installation tube 3. This ensures that the culture environment of the cell culture mother bottle 1 and the cell culture daughter bottle 2 is completely identical. Air is initially filtered through the filter screen 92 to remove large particles. HEPA filters can efficiently filter out micron-sized particles, including most microorganisms, greatly reducing the risk of microorganisms entering the culture flask and contaminating cells. This ensures a sterile environment for cell culture, reduces cell death or mutation due to contamination, and improves the purity of the gas entering the flask, preventing harmful gases or impurities from affecting cell culture. After the culture is completed, rotating the torsion block 103 drives the lead screw 102 to rotate, causing the moving block 105 to move within the slide groove 104. The moving block 105 drives the clamping block 106 to move, thereby disassembling the connecting tube 4 and facilitating the cleaning of the connecting tube 4, mother bottle 1, and daughter bottle 2.

Claims

1. A cell culture flask for preventing contamination, comprising a mother flask (1) and a daughter flask (2), characterized in that: The mother bottle (1) and the daughter bottle (2) are each fixedly connected to one side of an installation tube (3). A connecting tube (4) is provided between the two installation tubes (3). A clamping assembly (10) is provided on one side of the installation tube (3). The connecting tube (4) is fixedly connected to the installation tube (3) through the clamping assembly (10). A screw head (5) is fixedly connected to the top of the mother bottle (1) and the daughter bottle (2). A sealing cap (6) is threaded on the upper side of the screw head (5). A filter mechanism (9) is provided on one side of the sealing cap (6). A bottle stopper (7) is inserted into the inside of the screw head (5) of both the mother bottle (1) and the daughter bottle (2). An air exchange hole (8) is opened inside the bottle stopper (7).

2. The cell culture flask for preventing contamination according to claim 1, characterized in that: The filtration mechanism (9) includes a cover (91) which is fitted to the upper side of the sealing cover (6). A filter screen (92) is fixedly connected to the inside side of the cover (91). A HEPA filter (93) is fixedly connected to the cover (91) below the filter screen (92). An installation assembly (94) is provided between the cover (91) and the sealing cover (6).

3. A cell culture flask for preventing contamination according to claim 2, characterized in that: The mounting assembly (94) includes a plug (941) which is fixedly connected to both sides of the cover (91). Both sides of the sealing cover (6) are fixedly connected to a mounting frame (947). The mounting frame (947) has a slot (943) inside. The plug (941) is inserted into the slot (943). The plug (941) has a locking hole (942) inside. A locking block (944) is inserted into the locking hole (942).

4. A cell culture flask for preventing contamination according to claim 3, characterized in that: A spring (946) is fixedly connected inside the mounting frame (947), and a slider (945) is fixedly connected to one side of the spring (946). One side of the slider (945) is fixedly connected to one side of the locking block (944).

5. A cell culture flask for preventing contamination according to claim 4, characterized in that: Limiting grooves (948) are provided on both sides of the interior of the mounting frame (947), and one side of the slider (945) is slidably connected to the limiting groove (948).

6. A cell culture flask for preventing contamination according to claim 1, characterized in that: The clamping assembly (10) includes a fixing frame (101), which is fixedly connected to one side of the mounting pipe (3). The fixing frame (101) has a sliding groove (104) inside, and a moving block (105) is slidably connected inside the sliding groove (104). A clamping block (106) is fixedly connected to one side of the moving block (105).

7. A cell culture flask for preventing contamination according to claim 6, characterized in that: The slide groove (104) is rotatably connected to a lead screw (102), and a torsion block (103) is fixedly connected to the end of the lead screw (102). One side of the moving block (105) is threadedly connected to the lead screw (102).

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