A circulating carbon dioxide incubator

Abstract

This application discloses a circulating carbon dioxide incubator, belonging to the field of biological culture equipment technology. It includes an incubator body, an exhaust pipe, and an intake pipe. A limiting seat is fixed inside the incubator body, and a placement plate slides within the limiting seat. An exhaust box is fixed through the front end of the incubator body, and a first vent hole is opened on the exhaust box in the front area of ​​the lower placement plate. This circulating carbon dioxide incubator, by changing the traditional bottom humidification method, sets up humidification boxes inside the exhaust box and the conversion box. The positions of the humidification boxes correspond to the areas of different placement plates. A fan drives airflow circulation, allowing the moisture generated by the humidification boxes to be evenly distributed to all levels of the incubator. This solves the problem of uneven humidity distribution between different levels within the incubator, reduces dehydration of upper-layer samples and mold growth in lower-layer samples due to excessive humidity, and improves the biological sample culture effect.

Description

Technical Field

[0001] This application relates to the field of biological culture equipment technology, specifically a circulating carbon dioxide incubator. Background Technology

[0002] In modern biomedical research and the biotechnology industry, carbon dioxide incubators are indispensable key equipment. They provide suitable conditions for the growth, reproduction, and metabolism of biological samples by precisely controlling various parameters of the culture environment.

[0003] In some existing CO2 incubators, the humidification box or tray is mostly placed at the bottom of the incubator. This bottom humidification method tends to result in higher humidity in the lower layer and lower humidity in the upper layer. Especially in multi-layered structures, the humidity difference between biological samples in different layers may affect the culture effect. For example, samples in the upper layer may dehydrate due to insufficient humidity, while samples in the lower layer may grow mold due to excessive humidity. Therefore, it is necessary to design a circulating CO2 incubator to solve the above problems.

[0004] It should be noted that the information disclosed in this background section is only for understanding the background technology of this application concept, and therefore may include information that does not constitute prior art. Utility Model Content

[0005] Based on the aforementioned problems in the existing technology, the problem to be solved by this application is to provide a circulating carbon dioxide incubator, which solves the problem that the uneven humidity distribution of different levels in the incubator caused by the bottom humidification method in the existing carbon dioxide incubator, thereby affecting the culture effect of biological samples.

[0006] The technical solution adopted by this application to solve its technical problem is: a circulating carbon dioxide incubator, including an incubator body, an exhaust pipe and an intake pipe, wherein a limiting seat is fixed inside the incubator body and a placement plate slides inside the limiting seat;

[0007] The front end of the incubator is fixed with an exhaust box, and the exhaust box has a first vent hole located in the front area of ​​the lower placement plate. A first humidification box is slidably arranged inside the exhaust box.

[0008] The front and rear ends of the incubator are respectively connected to a conversion box. The conversion box has a second vent located in the rear end area of ​​the middle layer plate and the front end area of ​​the upper layer plate. A second humidification box is slidably arranged inside the conversion box.

[0009] The conversion box is located inside the incubator and is equipped with multiple first circulation pipes and second circulation pipes. The air inlets at the other ends of the first circulation pipes and second circulation pipes are connected to the circulating airflow inside the incubator.

[0010] A fan is installed on the upper part of the incubator. The air outlet of the fan is connected to the exhaust box through the exhaust pipe, and the air inlet of the fan is connected to the incubator through the air intake pipe.

[0011] Furthermore, the first and second circulation pipes at the rear end are arranged in the rear end region of the middle layer placement plate, and the first and second circulation pipes at the front end are arranged in the front end region of the upper layer placement plate.

[0012] Furthermore, the suction position of the suction tube inside the incubator is located at the rear end of the upper placement plate.

[0013] Furthermore, an air filter is installed in the body of the intake pipe.

[0014] Furthermore, both the first humidification box and the second humidification box are equipped with observation windows, which are made of transparent and heat-resistant material.

[0015] Furthermore, a protective door is hinged to one side of the incubator.

[0016] Furthermore, the protective door is equipped with an observation door.

[0017] The beneficial effects of this application are as follows: The circulating carbon dioxide incubator provided by this application changes the traditional bottom humidification method by setting up humidification boxes in the exhaust box and conversion box, and the position of the humidification boxes corresponds to the areas of different layer placement plates. The airflow is driven by a fan to circulate, so that the moisture generated by the humidification boxes can be evenly distributed to each layer of the incubator with the airflow. This solves the problem of uneven humidity distribution in different layers of the incubator, reduces the occurrence of dehydration of upper layer samples and mold growth of lower layer samples due to excessive humidity, and improves the culture effect of biological samples. Attached Figure Description

[0018] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. In the drawings:

[0019] Figure 1 This is a first three-dimensional structural schematic diagram of a circulating carbon dioxide incubator according to an embodiment of this application;

[0020] Figure 2 This is a second three-dimensional structural schematic diagram of a circulating carbon dioxide incubator according to an embodiment of this application;

[0021] Figure 3 This is a cross-sectional view of the exhaust box and the conversion box according to an embodiment of this application;

[0022] Figure 4This is a first assembly diagram showing the first and second circulation tubes located inside the incubator according to an embodiment of this application;

[0023] Figure 5 This is a second assembly diagram showing the first and second circulation tubes located inside the incubator according to an embodiment of this application.

[0024] The following are the labeling elements in the figure:

[0025] 1. Incubator; 2. Protective door; 3. Observation door; 4. Limiting seat; 5. Placement plate; 6. Exhaust box; 7. First vent; 8. First humidification box; 9. Observation window; 10. Transfer box; 11. Second vent; 12. Second humidification box; 13. First circulation pipe; 14. Second circulation pipe; 15. Fan; 16. Exhaust pipe; 17. Intake pipe; 18. Air filter. Detailed Implementation

[0026] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0027] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0028] like Figures 1-5As shown, this application provides a circulating carbon dioxide incubator, including an incubator body 1, a limiting seat 4, a placement plate 5, an exhaust box 6, a transfer box 10, a first circulation pipe 13, a second circulation pipe 14, a fan 15, an exhaust pipe 16, and an intake pipe 17. The incubator body 1 adopts a double-layer stainless steel structure. The inner layer is 304 stainless steel with electrolytic polishing treatment for easy cleaning and disinfection; the outer layer is Q235 carbon steel with a thickness of 2mm and powder coating treatment, providing good heat insulation and rust prevention. A 100mm thick polyurethane insulation material with a thermal conductivity ≤0.024W / (m・K) is filled between the two layers to ensure the incubator's heat preservation performance and reduce heat loss. The internal space of the incubator 1 is divided into three layers, with three placement plates 5 mounted on them via limiting seats 4. The limiting seats 4 are stainless steel supports welded to the inner wall of the incubator 1. The placement plates 5 are stainless steel mesh plates with a mesh aperture of 5mm, ensuring both airflow and stable placement of culture vessels. The incubator 1 is used to maintain the culture environment for cells, tissues, etc., by providing stable temperature, humidity, carbon dioxide concentration, and sterile conditions. Specifically, the temperature is regulated through a circulation system, carbon dioxide concentration is monitored by sensors and feedback is used to control the air intake, combined with humidity regulation and sterilization devices to create a suitable culture environment.

[0029] The exhaust box 6, made of stainless steel, is welded and fixed to the front end of the incubator body 1. The exhaust box 6 has a first vent 7 with a diameter of 10mm, arranged in a matrix pattern, corresponding to the front area of ​​the lower placement plate 5. A first humidification box 8, a cuboid structure made of PP plastic, slides inside the exhaust box 6, exhibiting good corrosion resistance and high-temperature resistance (able to withstand sterilization at 121℃). The first humidification box 8 can hold sterile water or other humidifying liquids, providing moisture to the airflow through water evaporation. When the first humidification box 8 slides to its installation position within the exhaust box 6, it is secured with bolts, ensuring a tight seal between the first humidification box 8 and the exhaust box 6.

[0030] The conversion boxes 10 are respectively welded and fixed to the front and rear ends of the incubator body 1. The front conversion box 10 corresponds to the front area of ​​the upper placement plate 5, and the rear conversion box 10 corresponds to the rear area of ​​the middle placement plate 5. The conversion box 10 uses the same material and process as the exhaust box 6. A second vent 11 with a diameter of 8mm is opened on the conversion box 10, corresponding to the rear area of ​​the middle placement plate 5 and the front area of ​​the upper placement plate 5. A second humidification box 12 is slidably installed inside the conversion box 10. The material is the same as the first humidification box 8, which is convenient for replacement and cleaning. At the same time, when the second humidification box 12 slides into the installation position inside the conversion box 10, it is fixed by bolts, thereby ensuring the sealing effect between the second humidification box 12 and the conversion box 10.

[0031] Both the first circulation pipe 13 and the second circulation pipe 14 are made of stainless steel with a diameter of 25 mm and a wall thickness of 1.2 mm. The rear end of the first circulation pipe 13 and the second circulation pipe 14 are arranged in the rear area of ​​the middle layer placement plate 5, and the front end of the first circulation pipe 13 and the second circulation pipe 14 are arranged in the front area of ​​the upper layer placement plate 5. The air inlet of the circulation pipe faces the inside of the incubator 1 to facilitate the intake of circulating airflow; the air outlet is connected to the conversion box 10 to ensure that the airflow enters the conversion box 10 smoothly. The number of circulation pipes is set according to the size of the incubator 1. In this embodiment, 4 first circulation pipes 13 and 3 second circulation pipes 14 are set in each layer, evenly distributed in the placement plate area.

[0032] Fan 15 is installed on the upper part of incubator 1. It is a brushless DC fan, model EC130, with a rated power of 60W, a maximum air volume of 150m³ / h, and a noise level ≤45dB, suitable for laboratory environments. The air outlet of fan 15 is connected to exhaust box 6 through exhaust pipe 16. Exhaust pipe 16 is a 50mm diameter PVC pipe with an insulation layer to prevent airflow temperature loss. The air inlet is connected to incubator 1 through suction pipe 17. Suction pipe 17 is located at the rear end of upper placement plate 5 within incubator 1, ensuring that upper air is drawn in to form a three-dimensional circulation. Air filter 18 is installed on the body of suction pipe 17. The filter uses an H14 grade high-efficiency filter membrane with a filtration efficiency ≥99.995% (for 0.3μm particles), ensuring that the air entering fan 15 is clean and avoiding contamination of the culture environment.

[0033] Both the first humidification box 8 and the second humidification box 12 are equipped with observation windows 9. These windows are made of transparent polycarbonate (PC) material, 5mm thick, and offer good transparency and temperature resistance (withstanding temperatures from -40℃ to 120℃), facilitating observation of the water level within the humidification box and allowing for timely replenishment of moisture. A protective door 2 is hinged to one side of the incubator body 1. The protective door 2 has a double-layer structure and seals tightly with the incubator body 1. A silicone sealing ring is installed on the edge of the door to ensure the incubator's airtightness. An observation door 3, made of transparent glass, is installed on the protective door 2, allowing observation of the sample status inside the chamber without opening the protective door 2, minimizing interference with the culture environment.

[0034] Working principle: First, the fan 15 is started by connecting an external power supply and the corresponding controller. The fan 15 draws air from the rear end of the upper placement plate 5 inside the incubator 1 through the air intake pipe 17. The air is filtered by the air filter 18 on the air intake pipe 17 to remove impurities and microorganisms in the air and ensure the cleanliness of the air.

[0035] The filtered air enters the fan 15, and after being pressurized by the fan 15, it is delivered to the exhaust box 6 through the exhaust pipe 16. In the exhaust box 6, the airflow passes through the first humidification box 8, carrying the moisture in the first humidification box 8, and then enters the front area of ​​the lower placement plate 5 in the incubator 1 through the first vent 7 on the exhaust box 6, providing humidity for the biological samples in the lower layer.

[0036] The airflow entering the lower layer is drawn into the rear conversion box 10 through the rear first circulation pipe 13 and the rear second circulation pipe 14 under the negative pressure of the fan 15. The airflow in the rear conversion box 10 is humidified by the second humidification box 12 and then discharged to the rear area of ​​the middle layer placement plate 5 through the second vent 11.

[0037] The airflow from the rear end of the middle layer flows to the front end to form a lateral convection. Part of the airflow is drawn into the front conversion box 10 through the first circulation pipe 13 and the second circulation pipe 14 at the front end. After being humidified by the second humidification box 12, it is discharged to the front end area of ​​the upper placement plate 5 through the second vent 11. The humid airflow at the front end of the upper layer diffuses to the rear end, mixes with the air at the rear end of the upper layer, and is drawn in by the suction pipe 17. After being pressurized by the fan 15, it re-enters the exhaust pipe 16 to start a new cycle, realizing a three-dimensional circulation of "lower layer → middle layer → upper layer → fan 15 → lower layer", so that the humidity in the incubator 1 is uniform.

[0038] In this system, the airflow continuously circulates within the incubator 1, passing through the various levels of placement plates 5, evenly distributing temperature, humidity, and carbon dioxide concentration to all areas of the incubator 1. Simultaneously, throughout the entire circulation process, the suction pipe 17 continuously draws in air from the rear end of the upper placement plate 5, and the fan 15 continuously pressurizes and delivers the air to the exhaust box 6 and the conversion box 10. After humidification, the air is then circulated back into the incubator 1, forming a complete airflow circulation system to ensure that biological samples grow in a suitable environment.

[0039] The above are merely preferred embodiments of this application and are not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A circulating carbon dioxide incubator comprising an incubator body (1), an exhaust pipe (16) and an air suction pipe (17), characterized in that: The culture chamber (1) is fixed with a limiting seat (4), and a placement plate (5) slides inside the limiting seat (4). The front end of the incubator (1) is fixed with an exhaust box (6), and the exhaust box (6) is provided with a first ventilation hole (7) located in the front end area of ​​the lower placement plate (5). The first humidification box (8) is slidably arranged inside the exhaust box (6). The front and rear ends of the culture box (1) are respectively connected to the conversion box (10). The conversion box (10) is provided with a second ventilation hole (11) located in the rear end area of ​​the middle layer placement plate (5) and the front end area of ​​the upper layer placement plate (5). The conversion box (10) is slidably arranged with a second humidification box (12). The conversion box (10) is equipped with multiple first circulation pipes (13) and second circulation pipes (14) in the part inside the culture chamber (1). The air inlets at the other ends of the first circulation pipes (13) and second circulation pipes (14) are connected to the circulating airflow inside the culture chamber (1). A fan (15) is installed on the upper part of the culture chamber (1). The air outlet of the fan (15) is connected to the exhaust box (6) through the exhaust pipe (16), and the air inlet of the fan (15) is connected to the culture chamber (1) through the air inlet pipe (17).

2. The recirculating carbon dioxide incubator of claim 1, wherein: The first circulation pipe (13) and the second circulation pipe (14) at the rear end are arranged in the rear end area of ​​the middle layer placement plate (5), and the first circulation pipe (13) and the second circulation pipe (14) at the front end are arranged in the front end area of ​​the upper layer placement plate (5).

3. A circulating carbon dioxide incubator according to claim 1, characterized in that: The suction tube (17) is located at the rear end of the upper placement plate (5) inside the incubator (1).

4. A circulating carbon dioxide incubator according to claim 1, characterized in that: An air filter (18) is installed in the body of the intake pipe (17).

5. A circulating carbon dioxide incubator according to claim 1, characterized in that: Both the first humidification box (8) and the second humidification box (12) are equipped with observation windows (9), which are made of transparent heat-resistant material.

6. A circulating carbon dioxide incubator according to claim 1, characterized in that: A protective door (2) is hinged to one side of the incubator (1).

7. A circulating carbon dioxide incubator according to claim 6, characterized in that: An observation door (3) is provided on the protective door (2).

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