A cell culture and detection box for stem cell therapy

Abstract

This utility model relates to a cell culture testing box for stem cell therapy, and more particularly to a cell culture testing box for stem cell therapy, comprising a box body, a microscope, a second sealing door, and a culture medium. The microscope is fixed to the top of the box body, the second sealing door is rotatably connected to the side wall of the box body, a glove is fixed to the side wall of the box body, and a transfer box is embedded in the side wall of the box body near the glove. The end of the transfer box is rotatably connected to a first sealing door. Compared with traditional cell culture testing boxes for stem cell therapy, this utility model, through the cooperation of the transfer box, the first sealing door, the sliding cover, and the nozzle, can reduce the entry of external air and bacteria into the box body. The cooperation of the sliding cover and the sliding groove improves the stability of the sliding cover movement. The cooperation of the glove and the lever improves the convenience of opening the sliding cover, making it convenient for operators to pour the nutrient solution inside the transfer box into the culture medium, thereby improving the safety of the stem cell culture process and thus increasing the survival rate.

Description

Technical Field

[0001] This utility model relates to a cell culture testing box for stem cell therapy, and more particularly to a cell culture testing box for stem cell therapy. Background Technology

[0002] Stem cells are a type of cell with self-renewal and multi-lineage differentiation potential, and they have shown enormous application potential in the medical field. Stem cells can be obtained from adult tissues: adult stem cells can be isolated and extracted from bone marrow, peripheral blood, adipose tissue, umbilical cord blood, placenta, and other adult tissues. For example, hematopoietic stem cells and mesenchymal stem cells can be obtained from bone marrow through bone marrow aspiration; adipose-derived stem cells can be obtained from adipose tissue through liposuction; and hematopoietic stem cells can be isolated from umbilical cord blood. By culturing specific types of stem cells, such as cardiac stem cells, for the treatment of cardiovascular diseases, it is hoped that damaged tissues and organs can be repaired and their functions restored. A culture monitoring chamber is required during stem cell culture.

[0003] Existing stem cell therapy cell culture testing chambers typically include a chamber body, a door, and control and testing components. The control and testing components usually include a microscope, a temperature and humidity sensor, a single gas sensor, and a heater. The microscope is positioned directly above the stem cell culture medium for easy real-time monitoring by the operator. The temperature and humidity sensor is used to monitor the temperature and humidity inside the chamber in real time, and the single gas sensor is used to detect CO2 concentration, thereby improving the survival rate of stem cell culture.

[0004] In the use of existing stem cell culture testing chambers for stem cell therapy, operators usually need to open the chamber door to add nutrient solution. Opening the door may allow air to enter the chamber, which may contain bacteria and a large amount of CO2. This may cause air disturbance inside the chamber, thereby affecting the safety and survival rate of stem cell culture. Utility Model Content

[0005] To overcome the problem that existing stem cell culture testing chambers for stem cell therapy typically require operators to open the chamber door to add nutrient solution, opening the door may allow air to enter the chamber. This air may contain bacteria and a large amount of CO2, which may cause air disturbance inside the chamber, thus affecting the safety and survival rate of stem cell culture.

[0006] The technical solution of this utility model is as follows: a cell culture detection box for stem cell therapy, comprising a box body, a microscope, a second sealing door, and a culture medium. The microscope is fixed at the top of the box body, the second sealing door is rotatably connected to the side wall of the box body, a glove is fixed on the side wall of the box body, a transfer box is embedded in the side wall of the box body near the glove, a first sealing door is rotatably connected to the end of the transfer box, a sliding cover is slidably connected to the side wall of the transfer box near the glove, a lever is fixed on the side wall of the sliding cover, a groove is opened on the side wall of the transfer box, a nozzle is fixed at the inner top of the transfer box, a support frame is fixed on the inner side wall of the box body near the bottom of the transfer box, and the culture medium is placed on the surface of the support frame near the microscope.

[0007] Furthermore, the cross-section of the sliding cover is convex, and the external dimensions of the sliding cover are adapted to the internal dimensions of the sliding groove.

[0008] Furthermore, a magnetic block is snapped onto the side wall of the sliding cover near the nozzle, and the magnetic block is attracted to the transfer box.

[0009] Furthermore, a glass window is embedded in the middle of the second sealed door, and a sealing ring is snapped onto the side wall of the second sealed door near the box body.

[0010] Furthermore, the first sealing door is rectangular, and its external dimensions are adapted to the external dimensions of the transfer box. A handle is fixed on the side wall of the first sealing door, and a bolt is installed on the side wall of the first sealing door near the handle.

[0011] Furthermore, heating wires are inserted into the side wall of the enclosure near the support frame, and the heating wires are distributed in an S-shape. An air filter is fixed on the side wall of the enclosure away from the second sealing door.

[0012] Furthermore, a temperature and humidity sensor is fixedly installed on the inner wall of the box away from the transfer box, and an IR sensor is fixedly installed on the side wall of the box close to the temperature and humidity sensor.

[0013] Furthermore, a connecting pipe is snapped onto the side wall of the enclosure near the temperature and humidity sensor. Several nozzles are inserted into the side wall of the connecting pipe. A water tank is fixed to the side wall of the enclosure, and a water pump is fixed to the top of the water tank. Pipes are inserted into both ends of the water pump, and the pipes are connected to the water tank and the connecting pipe, respectively.

[0014] The beneficial effects of this utility model are:

[0015] Compared to traditional stem cell culture testing chambers for stem cell therapy, this device, through the combination of a transfer box, a first sealed door, a sliding cover, and a nozzle, reduces the entry of external air and bacteria into the chamber. The sliding cover and its groove enhance the stability of its movement, while the combination of gloves and a lever improves the ease of opening the sliding cover, allowing operators to easily pour the nutrient solution from the transfer box into the culture medium, thereby improving the safety of the stem cell culture process and increasing the survival rate. Furthermore, the device incorporates a magnetic block, whose magnetism enhances the stability of the sliding cover's closure and provides support and restraint, preventing the sliding cover from sliding out of the groove and improving the safety of opening the cover. Attached Figure Description

[0016] Figure 1 The diagram shown is a three-dimensional structural schematic of the cell culture and testing box for stem cell therapy according to this utility model.

[0017] Figure 2 The diagram shown is a schematic representation of the box structure of this utility model.

[0018] Figure 3 The diagram shown is a schematic representation of the microscope structure of this utility model.

[0019] Figure 4 The diagram shown is a schematic representation of the structure of the transfer box of this utility model.

[0020] Figure 5 The diagram shown is a schematic representation of the sliding cover structure of this utility model.

[0021] Explanation of reference numerals in the attached diagram: 1. Box body; 2. Glove; 3. Transfer box; 4. First sealed door; 5. Microscope; 6. Second sealed door; 7. Support frame; 8. Culture medium; 9. Heating wire; 10. Sliding cover; 11. Water tank; 12. Water pump; 13. Temperature and humidity sensor; 14. IR sensor; 15. Connecting pipe; 16. Nozzle; 17. Magnetic block; 18. Slide; 19. Paddle; 20. Air filter. Detailed Implementation

[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0023] Example 1

[0024] Please see Figures 1-5A cell culture testing box for stem cell therapy includes a box body 1, a microscope 5, a second sealing door 6, and a culture medium 8. The microscope 5 is fixed to the top of the box body 1. The second sealing door 6 is rotatably connected to the side wall of the box body 1. A glove 2 is fixed to the side wall of the box body 1. A transfer box 3 is embedded in the side wall of the box body 1 near the glove 2. A temperature and humidity sensor 13 is fixed to the inner side wall of the box body 1 away from the transfer box 3 to detect temperature and humidity. An IR sensor 14 is fixed to the side wall of the box body 1 near the temperature and humidity sensor 13 to detect CO2 content. A first sealing door is rotatably connected to the end of the transfer box 3. Door 4 and transfer box 3 are slidably connected to the side wall near glove 2. A lever 19 is fixed on the side wall of the sliding cover 10. A sliding groove 18 is opened on the side wall of the transfer box 3. A nozzle 16 is fixed on the inner top of the transfer box 3. A support frame 7 is fixed on the inner side wall of the box body 1 near the bottom of the transfer box 3. A heating wire 9 is inserted into the side wall of the box body 1 near the bottom of the support frame 7. The heating wire 9 is distributed in an S-shape. An air filter 20 is fixed on the side wall of the box body 1 away from the second sealing door 6. The air filter 20 is connected to the inside of the box body 1. The culture medium 8 is placed on the surface of the support frame 7 near the microscope 5.

[0025] The sliding cover 10 has a convex cross-section, and its external dimensions are compatible with the internal dimensions of the slide groove 18. The second sealing door 6 has a glass window embedded in the middle, and a sealing ring is snapped onto the side wall of the second sealing door 6 near the box 1. The first sealing door 4 is rectangular, and its external dimensions are compatible with the external dimensions of the transfer box 3. A handle is fixed on the side wall of the first sealing door 4, and a bolt is installed on the side wall of the first sealing door 4 near the handle.

[0026] When using this stem cell therapy cell culture testing chamber, the operator first connects the external power supply, then places the culture medium 8 containing stem cells and nutrient solution on top of the support frame 7, and then rotates the second sealing door 6 to close the chamber 1, thus achieving sealed preservation. The operator can observe the internal state of the chamber 1 through the glass window on the surface of the second sealing door 6. The operator can conveniently detect the activity of stem cell culture by adjusting the eyepiece of the microscope 5. Turning on the power to the heating wire 9 generates heat, thereby maintaining a constant internal temperature of the chamber 1. The air filter 20 is used to filter and replenish oxygen inside the chamber 1. When culture medium needs to be added... The operator can rotate and open the first sealing door 4, place the container containing the culture medium at the bottom of the transfer box 3, and then close the first sealing door 4. Activating the nozzle 16 can kill bacteria inside the transfer box 3. The operator then inserts their hand into the inside of the glove 2, which is made of rubber and has good elasticity and sealing properties. The operator can then turn the lever 19 to open the transfer box 3 and remove the container containing the culture medium. The container can be poured into the culture medium 8. The internal space of the transfer box 3 is limited. After sterilization by the nozzle 16, air disturbance can be reduced, thereby improving the safety of the stem cell culture process and increasing the survival rate.

[0027] Example 2

[0028] Please see Figures 2-5 A magnetic block 17 is snapped onto the side wall of the sliding cover 10 near the nozzle 16, and the magnetic block 17 is attracted to the transfer box 3.

[0029] A connecting pipe 15 is snapped onto the side wall of the housing 1 near the temperature and humidity sensor 13. Several nozzles 16 are inserted into the side wall of the connecting pipe 15. A water tank 11 is fixed to the side wall of the housing 1. A water pump 12 is fixed to the top of the water tank 11. Pipes are inserted into both ends of the water pump 12. The pipes are connected to the water tank 11 and the connecting pipe 15, respectively. When the water pump 12 is started, distilled water inside the water tank 11 can be drawn out and sprayed out through the connecting pipe 15 and the nozzles 16, thereby facilitating humidity adjustment.

[0030] When the sliding cover 10 is sliding, the magnetic block 17 can provide support and limit, thereby preventing the sliding cover 10 from sliding out of the slide groove 18. The magnetic block 17 is magnetic and is attracted to the side wall of the transfer box 3, thereby improving the stability of the sliding cover 10 in closing.

[0031] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A cell culture detection case for stem cell therapy, characterized by, The container includes a box (1), a microscope (5), a second sealing door (6), and a culture medium (8). The microscope (5) is fixed at the top of the box (1). The second sealing door (6) is rotatably connected to the side wall of the box (1). A glove (2) is fixed on the side wall of the box (1). A transfer box (3) is embedded in the side wall of the box (1) near the glove (2). A first sealing door (4) is rotatably connected to the end of the transfer box (3). A sliding cover (10) is slidably connected to the side wall of the transfer box (3) near the glove (2). A lever (19) is fixed on the side wall of the sliding cover (10). A groove (18) is opened on the side wall of the transfer box (3). A nozzle (16) is fixed on the top inner part of the transfer box (3). A support frame (7) is fixed on the inner side wall of the box (1) near the bottom of the transfer box (3). The culture medium (8) is placed on the surface of the support frame (7) near the microscope (5).

2. The cell culture inspection case for stem cell treatment according to claim 1, wherein: The sliding cover (10) has a convex cross-section, and the external dimensions of the sliding cover (10) are matched with the internal dimensions of the slide groove (18).

3. The cell culture inspection case for stem cell treatment according to claim 2, wherein: A magnetic block (17) is snapped onto the side wall of the sliding cover (10) near the nozzle (16), and the magnetic block (17) is attracted to the transfer box (3).

4. The cell culture inspection case for stem cell treatment according to claim 1, wherein: The second sealing door (6) has a glass window embedded in the middle, and a sealing ring is attached to the side wall of the second sealing door (6) near the box (1).

5. The cell culture inspection case for stem cell treatment according to claim 1, wherein: The first sealing door (4) is rectangular. The external dimensions of the first sealing door (4) are adapted to the external dimensions of the transfer box (3). A handle is fixed on the side wall of the first sealing door (4), and a bolt is installed on the side wall of the first sealing door (4) near the handle.

6. The cell culture inspection case for stem cell treatment according to claim 1, wherein: A heating wire (9) is inserted into the side wall of the box (1) near the support frame (7). The heating wire (9) is distributed in an S-shape. An air filter (20) is fixed on the side wall of the box (1) away from the second sealing door (6).

7. The cell culture inspection case for stem cell treatment according to claim 1, characterized by: A temperature and humidity sensor (13) is fixed on the inner wall of the box (1) away from the transfer box (3), and an IR sensor (14) is fixed on the side wall of the box (1) close to the temperature and humidity sensor (13).

8. The cell culture inspection case for stem cell treatment according to claim 7, characterized by: A connecting pipe (15) is attached to the side wall of the housing (1) near the temperature and humidity sensor (13). Several nozzles (16) are inserted into the side wall of the connecting pipe (15). A water tank (11) is fixed on the side wall of the housing (1). A water pump (12) is fixed at the top of the water tank (11). Pipes are inserted at both ends of the water pump (12). The pipes are connected to the water tank (11) and the connecting pipe (15) respectively.

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