Continuous online viral inactivation, incubation system

Abstract

The utility model belongs to virus inactivation technical field discloses a kind of continuous online virus inactivation, incubation system, including sample stirrer, first delivery pump, first inactivator storage tank, second delivery pump, mixed stirrer, first four-way valve, concentration module, third delivery pump, first online detection module, second four-way valve, incubation module, second online detection module and finished product collection tank.Sample in sample stirrer and inactivator in first inactivator storage tank are continuously delivered to mixed stirrer according to required proportion, after mixing, it can be selected to enter concentration module concentration and enter first online detection module or directly enter first online detection module and carry out detection, the solution after detection can be selected to enter incubation module, after incubation, enter second online detection module or directly enter second online detection module and carry out detection, after detection, enter finished product collection tank and collect.The continuous online virus inactivation, incubation system can realize the automation of virus inactivation and incubation.

Description

Technical Field

[0001] This utility model relates to the field of virus inactivation technology, and in particular to a continuous online virus inactivation and incubation system. Background Technology

[0002] The primary purpose of virus inactivation during the manufacturing of biological products is to ensure the safety of the final product, prevent viral contamination from posing an infection risk to patients, and meet stringent regulatory requirements.

[0003] Traditional virus inactivation methods typically involve collecting samples offline in batches, then manually or by pumping the samples to mix them with a virus inactivation solution for pH adjustment or solvent addition, before transferring them to an incubation device for incubation. This method cannot achieve continuous automated sample processing, has low virus inactivation efficiency, and incurs high labor costs.

[0004] Therefore, there is an urgent need to propose a continuous online virus inactivation and incubation system to solve the above-mentioned technical problems. Utility Model Content

[0005] This invention provides a continuous online virus inactivation and incubation system that enables continuous automated processing of samples, improves the efficiency of virus inactivation, saves labor costs, and offers high flexibility to meet various user needs.

[0006] To achieve this objective, the present invention adopts the following technical solution:

[0007] A continuous online virus inactivation and incubation system includes a sample stirrer, a first delivery pump, a first inactivating agent storage tank, a second delivery pump, a mixing stirrer, a first four-way valve, a concentration module, a third delivery pump, a first online detection module, a second four-way valve, an incubation module, a second online detection module, and a finished product collection tank.

[0008] The inlet of the sample stirrer is connected to the sample output device, the outlet of the sample stirrer is connected to the inlet of the first delivery pump, and the outlet of the first delivery pump is connected to the first inlet of the mixing stirrer.

[0009] The outlet of the first inactivating agent storage tank is connected to the inlet of the second delivery pump, and the outlet of the second delivery pump is connected to the second inlet of the mixing agitator;

[0010] The outlet of the mixer is connected to the first port of the first four-way valve, the second port of the first four-way valve is connected to the inlet of the concentration module, the outlet of the concentration module is connected to the inlet of the third delivery pump, and the outlet of the third delivery pump is connected to the third port of the first four-way valve.

[0011] The fourth port of the first four-way valve is connected to the detection inlet of the first online detection module, the detection outlet of the first online detection module is connected to the first port of the second four-way valve, the second port of the second four-way valve is connected to the inlet of the incubation module, and the outlet of the incubation module is connected to the third port of the second four-way valve.

[0012] The fourth port of the second four-way valve is connected to the detection inlet of the second online detection module, and the detection outlet of the second online detection module is connected to the inlet of the finished product collection tank.

[0013] Optionally, the first online detection module includes a first pH detection unit and a first conductivity detection unit; the fourth port of the first four-way valve is connected to the inlet of the first pH detection unit, the outlet of the first pH detection unit is connected to the inlet of the first conductivity detection unit, and the outlet of the first conductivity detection unit is connected to the first port of the second four-way valve.

[0014] Alternatively, the first online detection module includes a first UV detection unit, a first pH detection unit, and a first conductivity detection unit; the fourth port of the first four-way valve is connected to the inlet of the first UV detection unit, the outlet of the first UV detection unit is connected to the inlet of the first pH detection unit, the outlet of the first pH detection unit is connected to the inlet of the first conductivity detection unit, and the outlet of the first conductivity detection unit is connected to the first port of the second four-way valve.

[0015] Optionally, the second online detection module includes a second UV detection unit, a second pH detection unit, and a second conductivity detection unit; the fourth port of the second four-way valve is connected to the inlet of the second UV detection unit, the outlet of the second UV detection unit is connected to the inlet of the second pH detection unit, the outlet of the second pH detection unit is connected to the inlet of the second conductivity detection unit, and the outlet of the second conductivity detection unit is connected to the inlet of the finished product collection tank.

[0016] Optionally, the continuous online virus inactivation and incubation system further includes a neutralization liquid storage tank, a first solenoid valve, a fourth delivery pump, and a neutralization stirrer; the outlet of the neutralization liquid storage tank is connected to the inlet of the first solenoid valve, the outlet of the first solenoid valve is connected to the inlet of the fourth delivery pump, the outlet of the fourth delivery pump is connected to the inlet of the neutralization stirrer, and the outlet of the neutralization stirrer is connected to the inlet of the second pH detection unit;

[0017] The outlet of the second UV detection unit is connected to the inlet of the second pH detection unit through a first pipeline. A second solenoid valve is provided on the first pipeline. The outlet of the second UV detection unit is also connected to the inlet of the neutralizing stirrer through a second pipeline. A third solenoid valve is provided on the second pipeline.

[0018] Optionally, the inlet of the sample stirrer is connected to the sample output device via a third pipeline, and a fourth solenoid valve is provided on the third pipeline.

[0019] Optionally, the sample stirrer is connected to the first delivery pump via a fourth pipeline, and a fifth solenoid valve is provided on the fourth pipeline;

[0020] The first inactivating agent storage tank is connected to the second delivery pump via a fifth pipeline, and a sixth solenoid valve is provided on the fifth pipeline;

[0021] The second online detection module is connected to the finished product collection tank through a sixth pipeline, and a seventh solenoid valve is provided on the sixth pipeline.

[0022] Optionally, the sample stirrer is connected to the first delivery pump via a fourth pipeline, the fourth pipeline being equipped with a first three-way valve, the first and second ports of the first three-way valve being connected to the fourth pipeline, and the third port of the first three-way valve being connected to the outlet of the first inactivating agent storage tank.

[0023] The first inactivating agent storage tank is connected to the second delivery pump through a fifth pipeline, and a sixth solenoid valve is provided on the fifth pipeline.

[0024] Optionally, the second online detection module is connected to the finished product collection tank via a sixth pipeline, and a second three-way valve is provided on the sixth pipeline. The first and second ports of the second three-way valve are connected to the sixth pipeline.

[0025] Optionally, the mixing agitator is connected to the first interface of the first four-way valve via a seventh pipeline, and a third three-way valve is provided on the seventh pipeline, with the first and second interfaces of the third three-way valve connected to the seventh pipeline.

[0026] Optionally, the detection outlet of the first online detection module is connected to the first interface of the second four-way valve through an eighth pipeline, and a third four-way valve is provided on the eighth pipeline, with the first interface and the second interface of the third four-way valve connected to the eighth pipeline;

[0027] The continuous online virus inactivation and incubation system further includes a second inactivating agent storage tank, an eighth solenoid valve, and a fifth delivery pump. The outlet of the second inactivating agent storage tank is connected to the inlet of the eighth solenoid valve, the outlet of the eighth solenoid valve is connected to the inlet of the fifth delivery pump, and the outlet of the fifth delivery pump is connected to the fourth port of the third four-way valve.

[0028] The beneficial effects of this utility model are:

[0029] This invention provides a continuous online virus inactivation and incubation system, comprising a sample stirrer, a first delivery pump, a first inactivating agent storage tank, a second delivery pump, a mixing stirrer, a first four-way valve, a concentration module, a third delivery pump, a first online detection module, a second four-way valve, an incubation module, a second online detection module, and a finished product collection tank. The sample from the sample output device is continuously delivered to the sample stirrer and stirred until homogeneous, then delivered to the mixing stirrer. Simultaneously, the inactivating agent in the first inactivating agent storage tank is also continuously delivered to the mixing stirrer according to the required ratio. In the mixing stirrer, the sample and inactivating agent are continuously mixed. The mixed solution can be selected to enter the concentration module for concentration as needed. After concentration, it enters the first online detection module or directly enters the first online detection module for detection. After detection, the solution can be selected to enter the incubation module for incubation as needed. After incubation, it enters the second online detection module or directly enters the second online detection module for detection. After detection, it is collected in the finished product collection tank. This continuous online virus inactivation and incubation system can automate virus inactivation and incubation, improving the efficiency of virus inactivation. Furthermore, it allows users to choose whether to concentrate and incubate the solution according to actual needs, offering high flexibility and meeting various user requirements.

[0030] Stirring the sample with a sample stirrer facilitates thorough mixing of the sample with the inactivating agent, thereby improving the adequacy of virus inactivation to a certain extent. Attached Figure Description

[0031] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments of this utility model will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the content of the embodiments of this utility model and these drawings without creative effort.

[0032] Figure 1 This is a schematic diagram of the continuous online virus inactivation and incubation system provided in Embodiment 1 of this utility model;

[0033] Figure 2 This is a schematic diagram of the continuous online virus inactivation and incubation system provided in Embodiment 2 of this utility model;

[0034] Figure 3 This is a schematic diagram of a continuous online virus inactivation and incubation system provided in Embodiment 3 of this utility model;

[0035] Figure 4 This is a schematic diagram of another continuous online virus inactivation and incubation system provided in Embodiment 3 of this utility model;

[0036] Figure 5 This is a schematic diagram of the continuous online virus inactivation and incubation system provided in Embodiment 4 of this utility model.

[0037] In the picture:

[0038] 1. Sample stirrer; 2. First transfer pump; 3. First inactivating agent storage tank; 4. Second transfer pump; 5. Mixing stirrer; 6. First four-way valve; 7. Concentration module; 8. Third transfer pump; 9. First online detection module; 91. First UV detection unit; 92. First pH detection unit; 93. First conductivity detection unit; 10. Second four-way valve; 11. Incubation module; 12. Second online detection module; 121. Second UV detection unit; 122. Second pH detection unit; 123. Second conductivity detection unit; 13. Finished product collection tank; 14. Neutralization liquid storage tank; 15. First 16. Solenoid valve; 17. Fourth transfer pump; 18. Neutralizing stirrer; 19. First pipeline; 20. Second solenoid valve; 21. Third solenoid valve; 22. Third pipeline; 23. Fourth solenoid valve; 24. Fourth pipeline; 25. Fifth solenoid valve; 26. Fifth pipeline; 27. Sixth solenoid valve; 28. Sixth pipeline; 29. ​​Seventh solenoid valve; 30. First three-way valve; 31. Second three-way valve; 32. Seventh pipeline; 33. Third three-way valve; 34. Eighth pipeline; 35. Third four-way valve; 36. Second inactivating agent storage tank; 37. Eighth solenoid valve; 38. Fifth transfer pump. Detailed Implementation

[0039] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, not the entire structure.

[0040] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0041] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0042] In the description of this embodiment, the terms "upper," "lower," "left," and "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0043] Example 1

[0044] This embodiment provides a continuous online virus inactivation and incubation system that enables continuous automated processing of samples, improves the efficiency of virus inactivation, saves labor costs, and offers high flexibility to meet various user needs.

[0045] like Figure 1 As shown, the continuous online virus inactivation and incubation system includes a sample stirrer 1, a first delivery pump 2, a first inactivating agent storage tank 3, a second delivery pump 4, a mixing stirrer 5, a first four-way valve 6, a concentration module 7, a third delivery pump 8, a first online detection module 9, a second four-way valve 10, an incubation module 11, a second online detection module 12, and a finished product collection tank 13.

[0046] Specifically, the connection method of the above components is as follows:

[0047] The inlet of sample stirrer 1 is connected to the sample output device, and the outlet of sample stirrer 1 is connected to the inlet of the first delivery pump 2. The outlet of the first delivery pump 2 is connected to the first inlet of the mixing stirrer 5. The outlet of the first inactivating agent storage tank 3 is connected to the inlet of the second delivery pump 4, and the outlet of the second delivery pump 4 is connected to the second inlet of the mixing stirrer 5. The outlet of the mixer 5 is connected to the first port a of the first four-way valve 6, the second port b of the first four-way valve 6 is connected to the inlet of the concentration module 7, the outlet of the concentration module 7 is connected to the inlet of the third delivery pump 8, the outlet of the third delivery pump 8 is connected to the third port c of the first four-way valve 6, the fourth port d of the first four-way valve 6 is connected to the detection inlet of the first online detection module 9, the detection outlet of the first online detection module 9 is connected to the first port a of the second four-way valve 10, the second port b of the second four-way valve 10 is connected to the inlet of the incubation module 11, the outlet of the incubation module 11 is connected to the third port c of the second four-way valve 10, the fourth port d of the second four-way valve 10 is connected to the detection inlet of the second online detection module 12, and the detection outlet of the second online detection module 12 is connected to the inlet of the finished product collection tank 13.

[0048] The working process of this continuous online virus inactivation and incubation system is as follows:

[0049] The sample from the sample output device is continuously fed to the sample stirrer 1 for stirring. The uniformly stirred sample is then fed to the mixing stirrer 5 by the first delivery pump 2. At the same time, the inactivating agent in the first inactivating agent storage tank 3 is continuously fed to the mixing stirrer 5 by the second delivery pump 4 according to the required ratio. The sample and the inactivating agent are continuously mixed in the mixing stirrer 5.

[0050] If the mixed solution needs to be concentrated, the first port a and the second port b of the first four-way valve 6 are connected, as are the third port c and the fourth port d. In this way, the mixed solution enters the concentration module 7 for concentration via the first port a and the second port b of the first four-way valve 6. The concentrated solution then enters the first online detection module 9 for detection, enabling online data collection. If the mixed solution does not need to be concentrated, the first port a and the fourth port d of the first four-way valve 6 are connected. In this way, the mixed solution directly enters the first online detection module 9 for detection, enabling online data collection.

[0051] If the tested solution requires incubation, the first port a and the second port b of the second four-way valve 10 are connected, as are the third port c and the fourth port d. Thus, the solution tested by the first online detection module 9 enters the incubation module 11 through the first port a and the second port b of the second four-way valve 10 for further virus inactivation. After incubation, the solution enters the second online detection module 12 through the third port c and the fourth port d of the second four-way valve 10 for online data collection. If incubation is not required, the first port a and the fourth port d of the second four-way valve 10 are connected. Thus, the solution enters the second online detection module 12 through the first port a and the fourth port d of the second four-way valve 10 for online data collection. After testing, the solution enters the finished product collection tank 13 for centralized collection.

[0052] The continuous online virus inactivation and incubation system provided in this embodiment can realize continuous and automated processing of samples to inactivate and incubate the virus in the samples. Compared with the offline batch-by-batch method in the prior art, it has higher work efficiency, requires no manual intervention, saves labor costs, and can choose whether to concentrate and incubate the solution according to actual needs, which has high flexibility and can meet various user needs.

[0053] In addition, stirring the sample with sample stirrer 1 helps to ensure thorough mixing of the sample with the inactivating agent, which improves the adequacy of virus inactivation to a certain extent.

[0054] It is understandable that the inactivating agent in the first inactivating agent storage tank 3 can be selected according to the needs of the sample, and this application does not make any specific restrictions.

[0055] Optionally, see [link to relevant documentation] Figure 1 In one possible embodiment, the first online detection module 9 includes a first pH detection unit 92 and a first conductivity detection unit 93. The fourth port d of the first four-way valve 6 is connected to the inlet of the first pH detection unit 92, the outlet of the first pH detection unit 92 is connected to the inlet of the first conductivity detection unit 93, and the outlet of the first conductivity detection unit 93 is connected to the first port a of the second four-way valve 10. That is, the solution exiting from the fourth port d of the first four-way valve 6 enters the first online detection module 9 to sequentially detect pH and conductivity, and collects detection data online to track the virus inactivation status in real time.

[0056] Optionally, see [link to relevant documentation] Figure 1In another possible embodiment, the first online detection module 9 includes a first UV detection unit 91, a first pH detection unit 92, and a first conductivity detection unit 93. The fourth port d of the first four-way valve 6 is connected to the inlet of the first UV detection unit 91, the outlet of the first UV detection unit 91 is connected to the inlet of the first pH detection unit 92, the outlet of the first pH detection unit 92 is connected to the inlet of the first conductivity detection unit 93, and the outlet of the first conductivity detection unit 93 is connected to the first port a of the second four-way valve 10. That is, the solution exiting from the fourth port d of the first four-way valve 6 enters the first online detection module 9 to sequentially detect UV, pH, and conductivity, and collects detection data online to track the virus inactivation status in real time.

[0057] Optionally, see [link to relevant documentation] Figure 1 The second online detection module 12 includes a second UV detection unit 121, a second pH detection unit 122, and a second conductivity detection unit 123. The fourth port d of the second four-way valve 10 is connected to the inlet of the second UV detection unit 121; the outlet of the second UV detection unit 121 is connected to the inlet of the second pH detection unit 122; the outlet of the second pH detection unit 122 is connected to the inlet of the second conductivity detection unit 123; and the outlet of the second conductivity detection unit 123 is connected to the inlet of the finished product collection tank 13. That is, after the solution exiting from the fourth port d of the second four-way valve 10 enters the second online detection module 12, UV, pH, and conductivity are detected sequentially, and the detection data is collected online to track the virus inactivation status in real time.

[0058] Understandably, the second online detection module 12 is mainly for detecting the incubated solution.

[0059] Further, see also Figure 1 In this embodiment, the inlet of the sample stirrer 1 is connected to the sample output device through a third pipe 22, and a fourth solenoid valve 23 is provided on the third pipe 22. By controlling the opening and closing of the fourth solenoid valve 23, the inactivation and incubation of the virus can be controlled.

[0060] Further, see also Figure 1 The sample stirrer 1 is connected to the first delivery pump 2 via a fourth pipeline 24, and a fifth solenoid valve 25 is installed on the fourth pipeline 24. By controlling the opening and closing of the fifth solenoid valve 25, the delivery of the sample can be controlled.

[0061] Further, see also Figure 1 The first deactivating agent storage tank 3 is connected to the second delivery pump 4 via a fifth pipeline 26, on which a sixth solenoid valve 27 is installed. By controlling the opening and closing of the sixth solenoid valve 27, the delivery of the deactivating agent can be controlled.

[0062] Further, see also Figure 1 The second online detection module 12 is connected to the finished product collection tank 13 via a sixth pipeline 28, on which a seventh solenoid valve 29 is installed. By controlling the opening and closing of the seventh solenoid valve 29, the collection of finished products can be controlled.

[0063] Example 2

[0064] This embodiment provides a continuous online virus inactivation and incubation system, which is largely the same in structure as Embodiment 1, with improvements only. Therefore, only the differences between the two are described here, and the structures identical to those in Embodiment 1 will not be repeated. In this embodiment, the same or corresponding technical features as in Embodiment 1 are referred to by the same reference numerals.

[0065] Specifically, such as Figure 2 As shown, in this embodiment, the continuous online virus inactivation and incubation system further includes a neutralizing liquid storage tank 14, a first solenoid valve 15, a fourth delivery pump 16, and a neutralizing stirrer 17. The outlet of the neutralizing liquid storage tank 14 is connected to the inlet of the first solenoid valve 15, the outlet of the first solenoid valve 15 is connected to the inlet of the fourth delivery pump 16, the outlet of the fourth delivery pump 16 is connected to the inlet of the neutralizing stirrer 17, and the outlet of the neutralizing stirrer 17 is connected to the inlet of the second pH detection unit 122. Furthermore, the outlet of the second UV detection unit 121 is connected to the inlet of the second pH detection unit 122 via a first pipeline 18, on which a second solenoid valve 19 is installed. The outlet of the second UV detection unit 122 is also connected to the inlet of the neutralizing stirrer 17 via a second pipeline 20, on which a third solenoid valve 21 is installed.

[0066] In this embodiment, the continuous online virus inactivation and incubation system can neutralize the incubated solution to further eliminate the virus, thus meeting various user needs.

[0067] Understandably, the neutralization operation can be used or not depending on actual needs.

[0068] If neutralization is not required, the first solenoid valve 15 and the third solenoid valve 21 are closed, the second solenoid valve 19 is opened, and the fourth delivery pump 16 is shut down.

[0069] If a neutralization operation is required, the first solenoid valve 15 and the third solenoid valve 21 are opened, the second solenoid valve 19 is closed, and the fourth delivery pump 16 is started.

[0070] Since the neutralized solution does not require UV detection, and only pH and conductivity need to be detected, setting the neutralization solution delivery after the second UV detection unit 121 is beneficial to saving the use of neutralization solution.

[0071] Example 3

[0072] This embodiment provides a continuous online virus inactivation and incubation system, which is largely the same in structure as Embodiment 1 or Embodiment 2, with improvements only made based on Embodiment 1 or Embodiment 2. Therefore, only the differences are described here, and the structures identical to those in Embodiment 1 or Embodiment 2 will not be repeated. In this embodiment, the same or corresponding technical features as in Embodiment 1 or Embodiment 2 are referred to by the same reference numerals.

[0073] When a sample needs to be treated using the above-mentioned continuous online virus inactivation and incubation system, and it is necessary to change the sample or the type of inactivating agent, in order to avoid the residual solution in the continuous online virus inactivation and incubation system affecting the effect of the next sample treatment, it is necessary to rinse the continuous online virus inactivation and incubation system with a new inactivating agent.

[0074] Based on the above issues, such as Figure 3 As shown, in the continuous online virus inactivation and incubation system provided in this embodiment, the sample stirrer 1 is connected to the first delivery pump 2 via a fourth pipeline 24. A first three-way valve 30 is provided on the fourth pipeline 24. The first port a and the second port b of the first three-way valve 30 are connected to the fourth pipeline 24, and the third port c of the first three-way valve 30 is connected to the outlet of the first inactivating agent storage tank 3. The first inactivating agent storage tank 3 is connected to the second delivery pump 4 via a fifth pipeline 26. A sixth solenoid valve 27 is provided on the fifth pipeline 26.

[0075] With this setup, the third port c and the second port b of the first three-way valve 30 are connected, the sixth solenoid valve 27 is opened, the first port a and the fourth port d of the first four-way valve 6 are connected, and the first port a and the fourth port d of the second four-way valve 10 are connected. This allows the new inactivating agent in the first inactivating agent storage tank 3 to be used to flush the continuous online virus inactivation and incubation system.

[0076] It is worth noting that, for the scheme in Embodiment 2, the second solenoid valve 19 and the third solenoid valve 21 also need to be opened during rinsing.

[0077] Further, see also Figure 3 The second online detection module 12 is connected to the finished product collection tank 13 via a sixth pipeline 28. A second three-way valve 31 is installed on the sixth pipeline 28, with its first port a and second port b connected to the sixth pipeline 28. With this configuration, when the continuous online virus inactivation and incubation system is in flushing mode, the first port a and third port c of the second three-way valve 31 are connected to drain the waste liquid. When the continuous online virus inactivation and incubation system is in sample processing mode, the first port a and second port b of the second three-way valve 31 are connected to collect the finished product through the finished product collection tank 13. The structure is simple and easy to control.

[0078] Optionally, such as Figure 4 As shown, the mixing agitator 5 is connected to the first port a of the first four-way valve 6 through the seventh pipe 32. The seventh pipe 32 is equipped with a third three-way valve 33, and the first port a and the second port b of the third three-way valve 33 are connected to the seventh pipe 32.

[0079] By installing a third three-way valve 33 on the seventh pipeline 32, a portion of the deactivating agent used for rinsing can be discharged in advance, which helps improve rinsing efficiency. Specifically, during rinsing, the first port a of the third three-way valve 33 can be simultaneously connected to the second port b and the third port c; alternatively, during rinsing, the first port a of the third three-way valve 33 can be connected to the third port c first, and after rinsing for a period of time, the first port a of the third three-way valve 33 can be connected to the second port b. The operation can be adjusted according to actual needs, and this application does not impose specific limitations.

[0080] Example 4

[0081] This embodiment provides a continuous online virus inactivation and incubation system, which is largely the same in structure as Embodiment 3, with improvements only. Therefore, only the differences between the two are described here, and the structures identical to those in Embodiment 3 will not be repeated. In this embodiment, the same or corresponding technical features as in Embodiment 3 are referred to by the same reference numerals.

[0082] Specifically, such as Figure 5 As shown, in this embodiment, the detection outlet of the first online detection module 9 is connected to the first interface a of the second four-way valve 10 via an eighth pipeline 34. A third four-way valve 35 is provided on the eighth pipeline 34, and the first interface a and the second interface b of the third four-way valve 35 are connected to the eighth pipeline 34. Furthermore, this continuous online virus inactivation and incubation system also includes a second inactivating agent storage tank 36, an eighth solenoid valve 37, and a fifth delivery pump 38. The outlet of the second inactivating agent storage tank 36 is connected to the inlet of the eighth solenoid valve 37, the outlet of the eighth solenoid valve 37 is connected to the inlet of the fifth delivery pump 38, and the outlet of the fifth delivery pump 38 is connected to the fourth interface d of the third four-way valve 35.

[0083] It is understandable that the inactivating agent in the second inactivating agent storage tank 36 is the same as that in the first inactivating agent storage tank 3. By setting up the second inactivating agent storage tank 36 to deliver inactivating agent to the continuous online virus inactivation and incubation system, the first inactivating agent can assist in flushing the continuous online virus inactivation and incubation system.

[0084] Specifically, during flushing, the first port a and the third port c of the third four-way valve 35 are connected, as are the second port b and the fourth port d. In this way, the flushing waste liquid upstream of the second deactivating agent storage tank 36 can be discharged through the third port c of the third four-way valve 35, while the flushing liquid for the system downstream of the second deactivating agent storage tank 36 is supplied through the second deactivating agent storage tank 36. This arrangement helps to improve flushing efficiency.

[0085] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make various obvious changes, readjustments, and substitutions without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A continuous online virus inactivation and incubation system, characterized in that, It includes a sample stirrer (1), a first delivery pump (2), a first inactivating agent storage tank (3), a second delivery pump (4), a mixing stirrer (5), a first four-way valve (6), a concentration module (7), a third delivery pump (8), a first online detection module (9), a second four-way valve (10), an incubation module (11), a second online detection module (12), and a finished product collection tank (13); The inlet of the sample stirrer (1) is connected to the sample output device, the outlet of the sample stirrer (1) is connected to the inlet of the first delivery pump (2), and the outlet of the first delivery pump (2) is connected to the first inlet of the mixing stirrer (5). The outlet of the first inactivating agent storage tank (3) is connected to the inlet of the second delivery pump (4), and the outlet of the second delivery pump (4) is connected to the second inlet of the mixing agitator (5); The outlet of the mixing agitator (5) is connected to the first port of the first four-way valve (6), the second port of the first four-way valve (6) is connected to the inlet of the concentration module (7), the outlet of the concentration module (7) is connected to the inlet of the third delivery pump (8), and the outlet of the third delivery pump (8) is connected to the third port of the first four-way valve (6). The fourth port of the first four-way valve (6) is connected to the detection inlet of the first online detection module (9), the detection outlet of the first online detection module (9) is connected to the first port of the second four-way valve (10), the second port of the second four-way valve (10) is connected to the inlet of the incubation module (11), and the outlet of the incubation module (11) is connected to the third port of the second four-way valve (10). The fourth port of the second four-way valve (10) is connected to the detection inlet of the second online detection module (12), and the detection outlet of the second online detection module (12) is connected to the inlet of the finished product collection tank (13).

2. The continuous online virus inactivation and incubation system according to claim 1, characterized in that, The first online detection module (9) includes a first pH detection unit (92) and a first conductivity detection unit (93); the fourth port of the first four-way valve (6) is connected to the inlet of the first pH detection unit (92), the outlet of the first pH detection unit (92) is connected to the inlet of the first conductivity detection unit (93), and the outlet of the first conductivity detection unit (93) is connected to the first port of the second four-way valve (10); Alternatively, the first online detection module (9) includes a first UV detection unit (91), a first pH detection unit (92), and a first conductivity detection unit (93); the fourth port of the first four-way valve (6) is connected to the inlet of the first UV detection unit (91), the outlet of the first UV detection unit (91) is connected to the inlet of the first pH detection unit (92), the outlet of the first pH detection unit (92) is connected to the inlet of the first conductivity detection unit (93), and the outlet of the first conductivity detection unit (93) is connected to the first port of the second four-way valve (10).

3. The continuous online virus inactivation and incubation system according to claim 1, characterized in that, The second online detection module (12) includes a second UV detection unit (121), a second pH detection unit (122), and a second conductivity detection unit (123); the fourth port of the second four-way valve (10) is connected to the inlet of the second UV detection unit (121), the outlet of the second UV detection unit (121) is connected to the inlet of the second pH detection unit (122), the outlet of the second pH detection unit (122) is connected to the inlet of the second conductivity detection unit (123), and the outlet of the second conductivity detection unit (123) is connected to the inlet of the finished product collection tank (13).

4. The continuous online virus inactivation and incubation system according to claim 3, characterized in that, The continuous online virus inactivation and incubation system further includes a neutralization liquid storage tank (14), a first solenoid valve (15), a fourth delivery pump (16), and a neutralization stirrer (17); the outlet of the neutralization liquid storage tank (14) is connected to the inlet of the first solenoid valve (15), the outlet of the first solenoid valve (15) is connected to the inlet of the fourth delivery pump (16), the outlet of the fourth delivery pump (16) is connected to the inlet of the neutralization stirrer (17), and the outlet of the neutralization stirrer (17) is connected to the inlet of the second pH detection unit (122); The outlet of the second UV detection unit (121) is connected to the inlet of the second pH detection unit (122) through the first pipe (18). The first pipe (18) is equipped with a second solenoid valve (19). The outlet of the second UV detection unit (121) is also connected to the inlet of the neutralizing stirrer (17) through the second pipe (20). The second pipe (20) is equipped with a third solenoid valve (21).

5. The continuous online virus inactivation and incubation system according to claim 1, characterized in that, The inlet of the sample stirrer (1) is connected to the sample output device through a third pipeline (22), and a fourth solenoid valve (23) is provided on the third pipeline (22).

6. The continuous online virus inactivation and incubation system according to any one of claims 1-5, characterized in that, The sample stirrer (1) is connected to the first delivery pump (2) through a fourth pipeline (24), and a fifth solenoid valve (25) is provided on the fourth pipeline (24); The first inactivating agent storage tank (3) is connected to the second delivery pump (4) through a fifth pipeline (26), and a sixth solenoid valve (27) is provided on the fifth pipeline (26); The second online detection module (12) is connected to the finished product collection tank (13) through a sixth pipeline (28), and a seventh solenoid valve (29) is provided on the sixth pipeline (28).

7. The continuous online virus inactivation and incubation system according to any one of claims 1-5, characterized in that, The sample stirrer (1) is connected to the first delivery pump (2) through a fourth pipeline (24). A first three-way valve (30) is provided on the fourth pipeline (24). The first and second ports of the first three-way valve (30) are connected to the fourth pipeline (24), and the third port of the first three-way valve (30) is connected to the outlet of the first inactivating agent storage tank (3). The first inactivating agent storage tank (3) is connected to the second delivery pump (4) through a fifth pipeline (26), and a sixth solenoid valve (27) is provided on the fifth pipeline (26).

8. The continuous online virus inactivation and incubation system according to claim 7, characterized in that, The second online detection module (12) is connected to the finished product collection tank (13) through the sixth pipeline (28). The sixth pipeline (28) is equipped with a second three-way valve (31), and the first and second ports of the second three-way valve (31) are connected to the sixth pipeline (28).

9. The continuous online virus inactivation and incubation system according to claim 7, characterized in that, The mixing agitator (5) is connected to the first interface of the first four-way valve (6) through the seventh pipeline (32). The seventh pipeline (32) is provided with a third three-way valve (33), and the first interface and the second interface of the third three-way valve (33) are connected to the seventh pipeline (32).

10. The continuous online virus inactivation and incubation system according to claim 7, characterized in that, The detection outlet of the first online detection module (9) is connected to the first interface of the second four-way valve (10) through the eighth pipeline (34). The eighth pipeline (34) is provided with a third four-way valve (35). The first interface and the second interface of the third four-way valve (35) are connected to the eighth pipeline (34). The continuous online virus inactivation and incubation system also includes a second inactivating agent storage tank (36), an eighth solenoid valve (37), and a fifth delivery pump (38). The outlet of the second inactivating agent storage tank (36) is connected to the inlet of the eighth solenoid valve (37), the outlet of the eighth solenoid valve (37) is connected to the inlet of the fifth delivery pump (38), and the outlet of the fifth delivery pump (38) is connected to the fourth port of the third four-way valve (35).

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