A magnetic bead stirring device

Abstract

The utility model discloses a kind of magnetic bead stirring devices, including base, stirring assembly, probe and receiver;Stirring assembly is installed on base;Base is provided with recess matched with container;At least a pair of probe and receiver are provided on the side wall of the recess.Through the probe, detection light source is emitted to receiver, and detection light source is received by receiver after passing through container and magnetic bead suspension in container;Through the intensity change of detection light source, the dispersion of magnetic bead suspension is judged, and according to dispersion, the parameter of stirring assembly is controlled.

Description

Technical Field

[0001] This utility model relates to the field of stirrer technology, specifically to a magnetic bead stirring device. Background Technology

[0002] The application of magnetic beads in fields such as biosensing and nucleic acid extraction requires maintaining uniform dispersion. Current technologies mostly employ mechanical or centrifugal stirring, suspending the magnetic beads in the solution. Biosensing or nucleic acid extraction is then performed via magnetic beads coupled together. However, current magnetic bead stirring methods suffer from the following problems: dispersion relies on manual experience for adjustment, lacking a quantitative feedback mechanism; and traditional conductivity and temperature monitoring methods cannot directly observe the distribution of magnetic bead particles, resulting in a lag in observation of the dispersion state and reduced reaction efficiency. Utility Model Content

[0003] In view of the above-mentioned technical problems existing in the prior art, the present invention provides a magnetic bead stirring device that can stir the magnetic beads while detecting the dispersion state of the magnetic beads.

[0004] This utility model discloses a magnetic bead stirring device, including a base, a stirring assembly, a probe, and a receiver; the stirring assembly is mounted on the base; the base is provided with a groove that mates with a container; at least one pair of probes and receivers are provided on the side wall of the groove.

[0005] Preferably, the emission wavelength of the probe is 850nm and / or 1300nm.

[0006] Preferably, a reflector is also provided on the side wall of the groove.

[0007] Preferably, a boss is provided in the groove; the container is detachably mounted on the boss.

[0008] Preferably, the present invention further includes a retainer installed in the groove; the retainer includes a base plate and a retaining plate spaced apart, and a connector is provided between the base plate and the retaining plate; the retaining plate is provided with a retaining hole that matches the container.

[0009] Preferably, the base plate is provided with a mounting groove that matches the bottom of the container.

[0010] Preferably, the base is provided with a support frame, and the stirring assembly includes a variable frequency motor and a stirring paddle;

[0011] The variable frequency motor is mounted on the bracket; the output end of the variable frequency motor is connected to the stirring paddle.

[0012] Preferably, the output end of the variable frequency motor is provided with an installation interface;

[0013] The connecting rod of the agitator is detachably mounted on the mounting interface;

[0014] The upper side of the base is provided with a mounting hole; the bracket is rotatably mounted in the mounting hole;

[0015] A locking mechanism that cooperates with the bracket is provided on one side of the base.

[0016] Preferably, it also includes a control module; the control module is connected to the probe, the receiver and the variable frequency motor respectively.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows: the probe emits a detection light source to the receiver, the detection light source passes through the container and the magnetic bead suspension in the container and is received by the receiver; by the intensity change of the detection light source, the dispersion of the magnetic bead suspension is determined, and the parameters of the stirring component are controlled according to the dispersion. Attached Figure Description

[0018] Figure 1 A schematic diagram of the magnetic bead stirring device of this utility model;

[0019] Figure 2 This is a schematic diagram of the probe and receiver installation;

[0020] Figure 3 It is a sectional view of the machine base;

[0021] Figure 4 This is a schematic diagram of the cage structure;

[0022] Figure 5 This is a schematic diagram showing the distribution of the probe and receiver;

[0023] Figure 6 This is a block diagram illustrating the control principle of a magnetic bead stirring device.

[0024] Markings in the diagram: 1. Magnetic bead stirring device; 2. Base; 21. Groove; 22. Boss; 23. Mounting hole; 3. Container; 31. Holder; 32. Base plate; 33. Connector; 35. Holder plate; 36. Holder hole; 37. Mounting slot; 4. Bracket;

[0025] 5. Mixing assembly; 51. Variable frequency motor; 52. Mixing paddle; 53. Connecting rod; 55. Mounting interface;

[0026] 6. Locking mechanism; 7. Probe; 71. Receiver; 72. Reflector; 8. Control module. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0028] The present invention will now be described in further detail with reference to the accompanying drawings:

[0029] like Figures 1-6 The magnetic bead stirring device 1 includes a base 2, a stirring assembly 5, a probe 7, and a receiver 71; the stirring assembly 5 is mounted on the base 2; the base 2 is provided with a groove 21 that mates with the container 3; for example... Figure 2 and Figure 5 At least one pair of probes 7 and receivers 71 are provided on the side wall of the groove 21.

[0030] The probe emits a detection light source to the receiver 71. The detection light source passes through the container 3 and the magnetic bead suspension inside the container 3 and is received by the receiver 71. By measuring the intensity change of the detection light source, the dispersion of the magnetic bead suspension is determined, and the parameters of the stirring component 5 are controlled according to the dispersion, such as adjusting the rotation speed.

[0031] The probe emits light at wavelengths of 850 nm and / or 1300 nm. Dual-wavelength or multi-wavelength probes are preferred.

[0032] like Figure 3 A reflector 72 is also provided on the side wall of the groove 21 to enhance the acquisition of light signals in the edge area. A boss 22 is provided inside the groove 21; the container 3 is detachably mounted on the boss 22. The groove 21 outside the boss 22 can be used to receive residual reagents or sputtered reagents, and is easy to clean.

[0033] A retainer 31 is provided within the groove 21 to hold the container 3 and prevent it, such as a centrifuge tube, from tilting. Figure 4 The retainer 31 includes a base plate 32 and a retaining plate 35 spaced apart, with a connector 33 between the base plate 32 and the retaining plate 35; the retaining plate 35 has retaining holes 36 that match the container 3. The base plate 32 has a mounting groove 37 that mates with the bottom of the container 3.

[0034] The base 2 is provided with a bracket 4, and the stirring assembly 5 includes a variable frequency motor 51 and a stirring paddle 52; the variable frequency motor 51 is mounted on the bracket 4; the output end of the variable frequency motor 51 is connected to the stirring paddle 52 and is used to drive the stirring paddle 52 to rotate.

[0035] The variable frequency motor 51 has an installation interface 55 at its output end; the connecting rod 53 of the stirring paddle 52 is detachably installed on the installation interface 55, such as by means of a buckle or a threaded ring; the upper side of the base 2 has an installation hole 23; the bracket 4 is rotatably installed in the installation hole 23; and a locking mechanism 6 that cooperates with the bracket 4 is provided on one side of the base 2, such as a locking pin.

[0036] Figure 6 The control principle of the magnetic bead stirring device is illustrated, and a control module 8 is designed. The control module 8 is connected to the probe 7, the receiver 71, and the variable frequency motor 51. It controls the probe 7 to emit detection light and receive signals from the receiver 71; based on the signals, it determines the change in light intensity and the uniformity of the magnetic bead suspension; and it controls the parameters of the variable frequency motor 51 according to the change in light intensity.

[0037] In a specific test, adjacent probes and / or receivers 71 are installed at a 30° interval, for a total of 6 sets of probes and 6 sets of receivers 71. The optical sampling period of the receiver 71 can be set to three levels: 100ms, 500ms, and 1s. The dynamic adjustment range of the variable frequency motor 51 is 50-1500 rpm (step accuracy ±5 rpm). The holder 31 is used to hold 50mL centrifuge tubes for DNA extraction testing via magnetic beads.

[0038] Based on the received light intensity, the dispersion D is calculated. When the dispersion D is greater than 0.95, the rotation speed is reduced to 0.7 times the current rotation speed, triggering speed reduction protection. When the dispersion D is less than 0.85, the rotation speed is increased to 1.3 times the current rotation speed. When the dispersion D is between 0.85 and 0.95, the rotation speed is the sum of the integral of the current rotation speed and the dispersion.

[0039] When the light intensity drops by more than 15%, a pulse acceleration mode is triggered, such as 3000 rpm / 0.5s.

[0040] This invention overcomes the reliance on manual observation methods when using magnetic beads, as it utilizes multiple probes to obtain real-time three-dimensional dispersion data. In specific tests, multi-dimensional optical data fusion improves dispersion uniformity by over 40%; closed-loop control delay is <200ms; it supports containers of various sizes from 50mL to 5000mL; reaction time is reduced to 60% of the original process; and energy consumption is reduced by 35%.

[0041] Additionally, a cover (not shown in the figure) can be installed on the support 4 to protect the stirring assembly 5. During use, the connector 33 should be kept out of reach of the probe light. For containers 3 that can be laid flat, the retainer 31 can be removed.

[0042] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A magnetic bead stirring device, characterized in that, It includes a base (2), a stirring assembly (5), a probe (7), and a receiver (71); The stirring assembly (5) is mounted on the base (2); The base (2) is provided with a groove (21) that mates with the container (3); At least one pair of probes (7) and receivers (71) are provided on the side wall of the groove (21).

2. The magnetic bead stirring device according to claim 1, characterized in that, The probe emits wavelengths of 850 nm and / or 1300 nm.

3. The magnetic bead stirring device according to claim 1, characterized in that, A reflector (72) is also provided on the side wall of the groove (21).

4. The magnetic bead stirring device according to claim 1, characterized in that, The groove (21) is provided with a boss (22); The container (3) is detachably mounted on the boss (22).

5. The magnetic bead stirring device according to claim 4, characterized in that, It also includes a retainer (31) installed in the groove (21); The retainer (31) includes a base plate (32) and a retaining plate (35) spaced apart. A connector (33) is provided between the base plate (32) and the retaining plate (35); The retaining plate (35) is provided with retaining holes (36) that match the container (3).

6. The magnetic bead stirring device according to claim 5, characterized in that, The base plate (32) is provided with an installation groove (37) that matches the bottom of the container (3).

7. The magnetic bead stirring device according to claim 1, characterized in that, A bracket (4) is provided on the base (2). The stirring assembly (5) includes a variable frequency motor (51) and a stirring paddle (52); The variable frequency motor (51) is mounted on the bracket (4); the output end of the variable frequency motor (51) is connected to the stirring paddle (52).

8. The magnetic bead stirring device according to claim 7, characterized in that, The output end of the variable frequency motor (51) is provided with an installation interface (55); The connecting rod (53) of the stirring paddle (52) is detachably mounted on the mounting interface (55); The base (2) is provided with a mounting hole (23) on its upper side; the bracket (4) is rotatably disposed in the mounting hole (23); A locking mechanism (6) that cooperates with the bracket (4) is provided on one side of the base (2).

9. The magnetic bead stirring device according to claim 1, characterized in that, It also includes a control module (8); The control module (8) is connected to the probe (7), receiver (71) and variable frequency motor (51) respectively.

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