A cell filtration screening device

Abstract

The utility model relates to cell filtering technical field, propose a kind of cell filtering screening device, the design includes support seat, support seat top is provided with main component, and support seat and main component junction is provided with shaking screening subassembly;Cover assembled in support seat top;Shaking screening subassembly includes the first motor that can drive main component and connecting plate and shake in support seat top level;First motor is fixedly installed in support seat interior;Main component includes support platform;The first sleeve for storing cell liquid before and after screening and the second sleeve are respectively fixedly connected in the both sides of support platform top;Pipetting assembly is arranged in support platform top for moving cell liquid from the first sleeve to the second sleeve in moving;The design is filtered by dynamic shaking acceleration, motor drive automatic pipetting and double-stage filter cartridge precision grading, solves the pain point of traditional technology low efficiency, operation is complicated, while realizing "one-stop" automated sorting in the promotion cell survival rate.

Description

Technical Field

[0001] This utility model relates to the field of cell filtration technology, specifically to a cell filtration screening device. Background Technology

[0002] Cell filtration screening is a key technology in the biomedical field, used to isolate specific cells from complex samples, and has wide applications in research and clinical practice. Its principle is based on differences in cell size, density, surface markers, and other characteristics, achieving separation through physical or immunological methods. Common methods include: mechanical filtration, which uses filter membranes of different pore sizes to separate cells according to size; simple to operate but with limited precision; density gradient centrifugation, which separates cells using media stratification and centrifugal force; suitable for blood cell separation but may affect viability; immunomagnetic bead sorting, which separates target cells through antibody labeling and magnetic fields; high purity but high cost; and flow cytometry sorting, which uses laser detection and charge deflection to achieve high-precision separation; suitable for complex samples but expensive equipment. This technology is widely used in basic research (such as stem cell isolation), clinical treatment (such as CAR-T cell preparation), and diagnostic testing (such as circulating tumor cell enrichment). During operation, attention must be paid to aseptic conditions, optimization of separation parameters to reduce cell damage, and verification of separation effectiveness to ensure the purity and viability of target cells. Choosing an appropriate method requires comprehensive consideration of experimental objectives, sample type, and equipment conditions. Utility Model Content

[0003] This invention proposes a cell filtration and screening device, which solves the problems in the prior art:

[0004] 1. Low filtration efficiency and easy cell damage: Traditional static filtration relies on gravity natural permeation, which takes a long time and cells are easily inactivated due to prolonged exposure;

[0005] 2. The graded screening process is cumbersome: multi-stage filtration requires manual sample transfer, which increases the risk of contamination and makes it difficult to ensure the accurate separation of cells of different particle sizes;

[0006] 3. Insufficient automation: Pipetting and shaking require manual operation, which is difficult to adapt to high-throughput screening requirements.

[0007] The technical solution of this utility model is as follows: A cell filtration and screening device includes a support base, a main body component is provided on the top of the support base, and a shaking screening component is provided at the connection between the support base and the main body component;

[0008] A snap-on cover fitted onto the top of the support;

[0009] The shaking screening component includes a first motor and a connecting plate that can drive the main component to shake horizontally on the top of the support.

[0010] The first motor is fixedly installed inside the support base;

[0011] The main component includes a support platform;

[0012] The top two sides of the support are respectively fixedly connected to a first sleeve and a second sleeve for storing cell fluid before and after screening;

[0013] A pipetting assembly disposed on top of the tray for transferring cell fluid from the first sleeve to the second sleeve.

[0014] Preferably, the shaking screening component further includes:

[0015] The main shaft is fixedly connected to the output end of the first motor;

[0016] The upper outer part of the main shaft is fixedly connected to the connecting plate.

[0017] Preferably, the shaking screening component further includes:

[0018] Rotary shaft connected to one side of the connecting plate;

[0019] The top of the secondary rotating shaft is fixedly connected to the bottom of the support platform.

[0020] Preferably, the shaking screening component further includes:

[0021] Rotate the ball bearings connected to the bottom of the support platform;

[0022] The bottom of the ball contacts the top of the support.

[0023] Preferably, the main component further includes:

[0024] A 60mm filter cartridge is fixedly connected inside the first sleeve;

[0025] The first inner ring is formed inside the 60mm filter cartridge;

[0026] The first outer ring is formed on the outside of the 60mm filter cartridge.

[0027] Preferably, the main component further includes:

[0028] A 30mm filter cartridge is fixedly connected inside the second sleeve;

[0029] A second inner ring is formed inside the second sleeve;

[0030] The second outer ring is formed on the outside of the second sleeve.

[0031] Preferably, the pipetting assembly includes:

[0032] Support plate and clips symmetrically fixedly connected to the back of the support plate;

[0033] The support plate is assembled onto the support platform via a clip.

[0034] Preferably, the pipetting assembly further includes:

[0035] A second motor is fixedly installed on the back of the support plate;

[0036] The inner rotating shaft is fixedly connected to the output end of the second motor;

[0037] The extrusion disc is fixedly connected to the outside of the inner rotating shaft.

[0038] Preferably, the pipetting assembly further includes:

[0039] A sleeve that is fixedly connected to the front of the support plate.

[0040] Preferably, the pipetting assembly further includes:

[0041] A pipette fixedly connected to the support plate inside the sleeve;

[0042] One end of the pipette is placed at the bottom of the first outer ring;

[0043] The other end of the pipette is placed inside the second inner ring.

[0044] The beneficial effects of this utility model are as follows:

[0045] I. Highly efficient graded screening and cell viability protection

[0046] 1. Dual-stage filter cartridge design: Large-sized impurities are initially removed by the first 60mm filter cartridge, and then the target cells are precisely separated by the second 30mm filter cartridge, achieving gradient grading screening.

[0047] 2. Dynamic shaking optimization: The shaking screening component is driven by the first motor to drive the connecting plate, which causes the support platform to shake horizontally (with ball bearing 23 assisting in stabilization), significantly accelerating the speed at which cell fluid penetrates the filter cartridge and reducing cell exposure damage.

[0048] II. Fully Automated Liquid Transfer and Contamination Prevention

[0049] 1. Integrated pipetting assembly: The second motor drives the squeezing disc to periodically squeeze the pipetting tube, automatically drawing in the initial screening cell fluid from the first outer ring and discharging it into the second inner ring, avoiding the risk of contamination from manual transfer.

[0050] 2. Modular mounting structure: The support plate is assembled by inserting and connecting the card holder, which facilitates quick disassembly and assembly of the pipette for cleaning or replacement.

[0051] III. Improved ease of use and stability

[0052] 1. Integrated design: The first sleeve, the second sleeve and the pipetting assembly are integrated on the platform, and a single device completes the entire process of shaking, filtering and transferring.

[0053] 2. Anti-tilt protection: The ball bearings at the bottom of the support base make rolling contact with the support seat to ensure smooth shaking and prevent cell fluid from splashing.

[0054] IV. Cost and Universality Advantages

[0055] 1. Purely mechanical structure: No need for expensive equipment such as flow cytometers, separation accuracy is controlled by the filter cartridge pore size, reducing the threshold for use in small and medium-sized laboratories.

[0056] 2. Adaptable to multiple scenarios: The filter cartridges with different pore sizes can be replaced to meet the sorting needs of different samples such as blood cells and tumor cells. Attached Figure Description

[0057] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0058] Figure 1 This is a schematic diagram of the overall device of this utility model;

[0059] Figure 2 This is a front view of the pipetting assembly of this utility model;

[0060] Figure 3 This is a schematic diagram of the back of the pipetting assembly of this utility model;

[0061] Figure 4 This is a schematic diagram of the shaking screening component of this utility model;

[0062] Figure 5 This is a schematic diagram of the main components of this utility model;

[0063] In the diagram: 1. Support base; 11. Cover; 2. Shaking screening assembly; 21. First motor; 211. Main shaft; 22. Connecting plate; 221. Secondary shaft; 23. Ball bearings; 3. Main assembly; 31. Support platform; 32. First sleeve; 321. 60mm filter cartridge; 3211. First inner ring; 3212. First outer ring; 33. Second sleeve; 331. 30mm filter cartridge; 3311. Second inner ring; 3312. Second outer ring; 4. Pipetting assembly; 41. Support plate; 411. Sleeve; 42. Second motor; 421. Inner shaft; 43. Squeezing plate; 44. Pipette; 45. Holder. Detailed Implementation

[0064] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this utility model.

[0065] Please see Figures 1-5 The present invention provides a technical solution: a cell filtration and screening device, including a support 1, a main body component 3 is provided on the top of the support 1, and a shaking screening component 2 is provided at the connection between the support 1 and the main body component 3.

[0066] The cover 11 is fitted onto the top of the support 1;

[0067] The shaking screening component 2 includes a first motor 21 capable of driving the main component 3 to shake horizontally on the top of the support 1 and a connecting plate 22;

[0068] The first motor 21 is fixedly installed inside the support 1;

[0069] The main component 3 includes a support platform 31;

[0070] The top two sides of the support platform 31 are respectively fixedly connected to the first sleeve 32 and the second sleeve 33 for storing the cell fluid before and after screening;

[0071] A pipetting assembly 4 is provided on the top of the tray 31 for transferring cell fluid from the first sleeve 32 to the second sleeve 33;

[0072] This design addresses the pain points of low efficiency and complicated operation of traditional technologies by using dynamic shaking to accelerate filtration, motor-driven automatic liquid transfer, and dual-stage filter cartridges for precise grading. It improves cell survival rate while achieving "one-stop" automated sorting.

[0073] Please see Figure 4 The shaking filter component 2 also includes:

[0074] The main shaft 211 is fixedly connected to the output end of the first motor 21;

[0075] The upper outer part of the main shaft 211 is fixedly connected to the connecting plate 22;

[0076] Shake-to-screen component 2 also includes:

[0077] Rotary shaft 221 is rotatably connected to one side of connecting plate 22;

[0078] The top of the secondary rotating shaft 221 is fixedly connected to the bottom of the support 31;

[0079] Shake-to-screen component 2 also includes:

[0080] Rotate the ball bearing 23 connected to the bottom of the support 31;

[0081] The bottom of ball 23 contacts the top of support 1;

[0082] The cell solution to be screened is first added to the inner side of the 60mm filter cartridge 321, located in the first inner ring 3211 area. Then, the first motor 21 is started to drive the main shaft 211 and the connecting plate 22 to rotate. By rotating the auxiliary shaft 221 connected to one side of the connecting plate 22, the support platform 31 can be driven to swing horizontally on the top of the support seat 1. This design can complete the shaking and screening of the cell solution.

[0083] In particular, this design provides rotatable ball bearings 23 at the bottom of the support platform 31. The support platform 31 is supported on the top of the support seat 1 by the ball bearings 23. This design can prevent the support platform 31 from tilting on the top of the support seat 1.

[0084] Please see Figure 2 and Figure 3 and Figure 5 The main component 3 also includes:

[0085] A 60mm filter cartridge 321 is fixedly connected inside the first sleeve 32;

[0086] The first inner ring 3211 is formed inside the 60mm filter cartridge 321;

[0087] The first outer ring 3212 is formed on the outside of the 60mm filter cartridge 321;

[0088] Main component 3 also includes:

[0089] A 30mm filter cartridge 331 is fixedly connected inside the second sleeve 33;

[0090] The second inner ring 3311 is formed inside the second sleeve 33;

[0091] The second outer ring 3312 is opened on the outside of the second sleeve 33;

[0092] Pipetting assembly 4 includes:

[0093] Support plate 41 and card holders 45 symmetrically fixedly connected to the back of support plate 41;

[0094] The support plate 41 is assembled onto the tray 31 via a clip 45.

[0095] The pipetting assembly 4 also includes:

[0096] A second motor 42 is fixedly installed on the back of the support plate 41;

[0097] The inner rotating shaft 421 is fixedly connected to the output end of the second motor 42;

[0098] The extrusion disc 43 is fixedly connected to the outside of the inner rotating shaft 421;

[0099] The pipetting assembly 4 also includes:

[0100] A sleeve 411 is fixedly connected to the front of the support plate 41;

[0101] The pipetting assembly 4 also includes:

[0102] A pipette 44 is fixedly connected to the support plate 41 and located inside the sleeve 411;

[0103] One end of the pipette 44 is placed at the bottom of the first outer ring 3212;

[0104] The other end of pipette 44 is placed inside the second inner ring 3311;

[0105] After the cell solution is shaken and screened in the first inner ring 3211, cell solutions smaller than 60 mm will pass through the 60 mm filter cartridge 321 and enter the first outer ring 3212. At this time, the second motor 42 is started to drive the inner rotating shaft 421 and the squeezing plate 43 to rotate. The squeezing plate 43 reciprocates to squeeze the pipette 44, which allows the cell solution in the first outer ring 3212 to be drawn in through one end of the pipette 44 and discharged from the other end of the pipette 44. At this time, the cell solution will fall into the second inner ring 3311. The first motor 21 continues to work to generate shaking, which allows the cell solution smaller than 30 mm in the second inner ring 3311 to pass through the 30 mm filter cartridge 331 and enter the second outer ring 3312. The cell solution that has passed through the 60 mm and 30 mm filters in sequence is pre-stored in the second outer ring 3312 and awaits to be taken out by the staff.

[0106] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A cell filtration and screening device, comprising a support (1), characterized in that: The support (1) is provided with a main body component (3) on its top, and a shaking screening component (2) is provided at the connection between the support (1) and the main body component (3). The cover (11) is fitted on the top of the support (1); The shaking screening component (2) includes a first motor (21) capable of driving the main component (3) to shake horizontally on the top of the support (1) and a connecting plate (22). The first motor (21) is fixedly installed inside the support (1); The main component (3) includes a support platform (31); The top two sides of the support (31) are respectively fixedly connected to a first sleeve (32) and a second sleeve (33) for storing cell fluid before and after screening. A pipetting assembly (4) is disposed on top of the tray (31) for transferring cell fluid from the first sleeve (32) to the second sleeve (33).

2. The cell filtration and screening device according to claim 1, characterized in that, The shaking screening component (2) also includes: The main shaft (211) is fixedly connected to the output end of the first motor (21). The upper outer part of the main shaft (211) is fixedly connected to the connecting plate (22).

3. The cell filtration and screening device according to claim 2, characterized in that, The shaking screening component (2) also includes: Rotary shaft (221) is rotatably connected to one side of the connecting plate (22); The top of the secondary rotating shaft (221) is fixedly connected to the bottom of the support (31).

4. The cell filtration and screening device according to claim 1, characterized in that, The shaking screening component (2) also includes: Rotate the ball bearing (23) connected to the bottom of the support (31); The bottom of the ball (23) is in contact with the top of the support (1).

5. The cell filtration and screening device according to claim 1, characterized in that, The main component (3) also includes: A 60mm filter cartridge (321) is fixedly connected inside the first sleeve (32); The first inner ring (3211) is formed inside the 60mm filter cartridge (321). The first outer ring (3212) is formed on the outside of the 60mm filter cartridge (321).

6. The cell filtration and screening device according to claim 1, characterized in that, The main component (3) also includes: A 30mm filter cartridge (331) is fixedly connected inside the second sleeve (33); The second inner ring (3311) is opened inside the second sleeve (33); The second outer ring (3312) is opened on the outside of the second sleeve (33).

7. The cell filtration and screening device according to claim 1, characterized in that, The pipetting assembly (4) includes: Support plate (41) and card holder (45) symmetrically fixedly connected to the back of the support plate (41); The support plate (41) is assembled on the tray (31) by inserting a bracket (45).

8. The cell filtration and screening device according to claim 7, characterized in that, The pipetting assembly (4) further includes: A second motor (42) is fixedly installed on the back of the support plate (41); The inner shaft (421) is fixedly connected to the output end of the second motor (42); The extrusion disc (43) is fixedly connected to the outside of the inner rotating shaft (421).

9. The cell filtration and screening device according to claim 7, characterized in that, The pipetting assembly (4) further includes: A sleeve (411) is fixedly connected to the front of the support plate (41).

10. A cell filtration and screening device according to claim 7, characterized in that, The pipetting assembly (4) further includes: A pipette (44) is fixedly connected to the support plate (41) on the inside of the sleeve (411). One end of the pipette (44) is placed at the bottom of the first outer ring (3212); The other end of the pipette (44) is placed inside the second inner ring (3311).

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