A portable extraction immersion type test paper roll for detecting hydrogen peroxide in milk

Abstract

The utility model provides a portable extraction infiltration formula test paper reel of hydrogen peroxide in milk is detected, has solved hydrogen peroxide detection method cost higher, not easy operation's problem. The utility model discloses test paper reel and hand -held liquid aspirator, test paper reel is the reel shape structure of front and back permeability, and test paper reel includes the backing layer at its outer layer and the test layer of fixedly established inside the backing layer, and the inside of test layer includes the C line coating and T line coating of front and back interval arrangement, and C line coating is located T line coating's rear, C line coating contains UCNPs coating, fluorescent donor coating in proper order from outside to inside, T line coating contains UCNPs coating, fluorescent donor coating, fluorescent acceptor coating in proper order from outside to inside, and fluorescent donor coating adopts NaYF4:Yb 3+ ,Er 3+ @SiO2 coating and is formed, and fluorescent acceptor coating adopts SQA-Fe 3+ Coating, and hand -held liquid aspirator includes liquid aspirator and the liquid pipe head of fixedly established liquid aspirator front end, and the rear end of test paper reel can be detachably equipped on the liquid pipe head.

Description

Technical Field

[0001] This utility model relates to the field of test strip technology, and in particular to a portable extractable immersion test strip roll for detecting hydrogen peroxide in milk. Background Technology

[0002] Hydrogen peroxide (H2O2) is a strong oxidizing agent with bleaching, disinfecting, and sterilizing properties, commonly used in chemical production and the food industry. In the food industry, H2O2 is used for packaging and sterilizing dairy products and extending the shelf life of milk. Studies have shown that excessive H2O2 in the human body can damage proteins, lipids, and nucleic acids, leading to cell apoptosis or necrosis, and may even trigger Alzheimer's disease, cancer, and cardiovascular disease. Residual H2O2 in milk poses a potential threat to human health, and my country explicitly prohibits the addition of H2O2 in dairy product production. Therefore, establishing a sensitive and rapid detection method for H2O2 in milk is of great significance for protecting the interests of consumers and ensuring human health.

[0003] Currently, methods for detecting H2O2 mainly include chemical titration analysis, electrochemical analysis, high-performance liquid chromatography (HPLC), spectrophotometry, and fluorescence analysis. Chemical titration analysis includes methods such as the potassium permanganate method, iodometric titration, and cerium titration. These methods have high sensitivity, but the potassium permanganate method has side reactions that can cause measurement deviations. In the iodometric titration, I2 is volatile and Na2S2O3 has poor stability, affecting the accuracy of the method. The cerium titration method has high cost for cerium salts and a narrow detection range. Electrochemical methods are highly sensitive and efficient, but the repeatability of the results is poor and the electrode life is limited. HPLC has high accuracy and sensitivity, but the instruments are expensive, sample pretreatment is complex, and the operating skills required of the operators are high; it also cannot achieve rapid on-site detection. In spectrophotometry, the titanium salt colorimetric method is a national standard detection method with advantages such as low cost and simple operation, but its stability needs further improvement. Therefore, establishing a sensitive, rapid, easy-to-operate, accurate, and low-cost method for detecting H2O2 residues is of great significance. Utility Model Content

[0004] To address the problems of high cost and difficulty in operation of current hydrogen peroxide detection methods in the background art, this utility model proposes a portable extractable immersion test paper roll for detecting hydrogen peroxide in milk.

[0005] The technical solution of this utility model is: a portable extractable immersion test strip roll for detecting hydrogen peroxide in milk, including a test strip roll and a handheld extractor;

[0006] The test strip roll is a roll-shaped structure that is open at both ends. The test strip roll includes a backing layer on its outer layer and a test layer fixed inside the backing layer. The interior of the test layer includes a C-line coating and a T-line coating that are spaced apart at both ends. The C-line coating is located behind the T-line coating.

[0007] The C-line coating consists of, from the outside in, a UCNPs coating and a fluorescent donor coating.

[0008] The T-line coating consists of, from the outside in, a UCNPs coating, a fluorescent donor coating, and a fluorescent acceptor coating.

[0009] The fluorescent donor coating uses NaYF4:Yb 3+ Er 3+ The coating is made of SiO2, and the fluorescent acceptor coating uses SQA-Fe. 3+ Coated;

[0010] The handheld liquid extractor includes a liquid extraction cylinder and a liquid extraction tube head fixed at the front end of the liquid extraction cylinder. The rear end of the test strip roll can be detachably fitted onto the liquid extraction tube head.

[0011] Preferably, the backing layer is made of a transparent and waterproof material.

[0012] Preferably, the backing layer has a cutting area that extends continuously in the front-to-back direction, and the test layer is fixed inside the cutting area and is broken at the cutting area.

[0013] Preferably, the inner side of the cutting area protrudes from the inner wall of the backing layer to form a strip structure extending in the vertical direction. The inner side of the strip structure is flush with the inner side of the test layer, and the ends of the test layer abut against the left and right sides of the strip structure.

[0014] Preferably, a cutting line is provided on the outer surface of the cutting area along its central axis.

[0015] Preferably, the test layer includes a sample pad, a nitrocellulose pad, an absorbent pad, and a pH test pad, which are fixedly disposed in a roll shape inside the backing layer from front to back, with the rear end of the sample pad and the front end of the absorbent pad respectively pressed onto the front and rear ends of the nitrocellulose pad.

[0016] The C-line coating and T-line coating are spaced apart on the inner side of the nitrocellulose pad.

[0017] Preferably, a detachable strap is fitted onto the liquid extraction tube head to secure the rear end of the test strip roll to the liquid extraction tube head.

[0018] Preferably, the liquid extraction cylinder is a syringe-like structure with a rear opening. The rear end of the liquid extraction cylinder is provided with a detachable rear cover. An electrically controlled push-pull device that can extend and retract back and forth is fixed on the front side of the rear cover. The electrically controlled push-pull device is located inside the liquid extraction cylinder. A piston is fixed on the front end of the electrically controlled push-pull device. The piston slides and fills inside the liquid extraction cylinder.

[0019] An electrical control box is fixedly installed on the back side of the back cover. A mobile power supply is installed inside the electrical control box. A push-out switch and a pull-back switch are fixedly embedded on the rear surface of the electrical control box. The mobile power supply is connected in series with the push-out switch and the pull-back switch respectively. The push-out switch and the pull-back switch are connected to the electric push-pull device respectively. The push-out switch and the pull-back switch are used to control the push-out and pull-back actions of the electric push-pull device respectively.

[0020] Advantages of this utility model: (1) This test paper roll detection method has high accuracy, high sensitivity, strong anti-interference ability, and simple operation. It does not require complicated labeling and incubation processes, providing a new idea and method for sensitive and rapid detection of H2O2 in food.

[0021] (2) Dry test paper rolls have a longer shelf life.

[0022] (3) Since the test strip roll contains specific values ​​of fluorescence signals, the detection results are more accurate compared to traditional colorimetric methods. Compared to traditional large-scale instrument detection methods, this patented method is more convenient and can achieve rapid on-site sample detection. At the same time, different detectors can be selected for detection according to different application scenarios: a portable fluorescence microplate reader with 980 nm excitation light and an F-4600 Hitachi fluorescence spectrophotometer with a solid sample holder can be used for analysis and detection.

[0023] (4) The test paper roll and hand-held liquid extractor are used together, which is faster, more direct and has a larger contact reaction area than the traditional capillary action paper-based test paper strip, thus realizing the on-site rapid specific classification, qualitative and quantitative detection of H2O2, and the detection time is shorter.

[0024] (5) The pH test pad at the rear end of the test strip roll can display the pH of the sample to be tested, determine whether the test conditions of the test strip are optimal, and determine whether the test results are relatively accurate. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the internal structure of Example 1;

[0027] Figure 2 for Figure 1 A schematic diagram of the external structure of the test paper roll;

[0028] Figure 3 for Figure 2 A top-down view of the planar structure;

[0029] Figure 4 for Figure 3 A structural schematic diagram of the BB cross section in the image;

[0030] Figure 5 for Figure 1 Enlarged view of the structure at point A in the image;

[0031] In the diagram, 1. Test strip roll, 2. Handheld liquid extractor, 3. Liquid extraction tube head, 4. Piston, 5. Electrically controlled push-pull device, 6. Back cover, 7. Electrical control box, 8. Push-out switch, 9. Pull-back switch, 10. Backing layer, 11. Cutting area, 12. Cutting line, 13. Test layer, 14. Nitrocellulose pad, 15. C-line coating, 16. T-line coating, 17. Sample pad, 18. Absorbent pad, 19. pH test pad, 20. Strap. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] Example 1: A portable, immersion-type test strip roll for detecting hydrogen peroxide in milk, such as... Figure 1 As shown, it includes a test strip roll 1 and a handheld liquid extractor 2.

[0034] like Figure 2 , Figure 3 and Figure 4 As shown, the test strip roll 1 is a roll-shaped structure that is open at both ends. The test strip roll 1 includes a backing layer 10 on its outer layer and a test layer 13 fixed inside the backing layer. In order to facilitate observation of the bonding between the test layer 13 and the liquid to be tested, to prevent the liquid to be tested from seeping out of the test layer 13, and to provide support for the wetted test layer 13, the backing layer 10 in this embodiment is made of transparent and waterproof polycarbonate material.

[0035] To facilitate cutting and avoid damaging test layer 13 during cutting, such as Figure 2 and Figure 3As shown, the backing layer 10 has a cutting area 11 extending continuously in the front-to-back direction. The inner side of the cutting area 11 protrudes from the inner wall of the backing layer 10 to form a strip structure extending in the vertical direction. The test layer 13 is fixedly disposed on the inner side of the cutting area 11 and is broken at the cutting area 11. The inner side of the strip structure is flush with the inner side of the test layer 13, and the ends of the test layer 13 abut against the left and right sides of the strip structure. A cutting line 12 is provided on the outer surface of the cutting area 11 along its central axis.

[0036] like Figure 4 As shown, the test layer 13 includes a sample pad 17, a nitrocellulose pad 14, an absorbent pad 18, and a pH test pad 19, which are fixedly disposed in a roll shape inside the backing layer 10 from front to back. The rear end of the sample pad 17 and the front end of the absorbent pad 18 are respectively pressed onto the front and rear ends of the nitrocellulose pad 14.

[0037] A pH test pad 19 is set at the rear end of the test strip roll 1 to display the pH of the sample to be tested and determine whether the test conditions are optimal.

[0038] The inner surface of the nitrocellulose pad 14 is coated with a C-line coating 15 and a T-line coating 16 spaced apart, with the C-line coating 15 located behind the T-line coating 16.

[0039] The C-line coating 15 includes, from the outside in, a UCNPs coating and a fluorescent donor coating.

[0040] The T-line coating 16 comprises, from the outside in, a UCNPs coating, a fluorescent donor coating, and a fluorescent acceptor coating.

[0041] The fluorescent donor coating uses NaYF4:Yb 3+ Er 3+ The coating is made of SiO2, and the fluorescent acceptor coating uses SQA-Fe. 3+ It is coated.

[0042] Selected NaYF4:Yb 3+ Er 3+ When used as a fluorescence donor, rare-earth-doped upconversion nanomaterials like SiO2 provide Yb with only one excited-state energy level. 2 F 7 / 2 The absorption band is at 980 nm in the near-infrared region. When Yb is irradiated by a 980 nm light source, Yb can transition to an excited state and transfer the absorbed energy to the activator.

[0043] NaYF4:Yb using Er as an activator 3+ Er 3+ The emission wavelength of SiO2 is 544 nm, which is similar to that of ferric squartz (squaric acid and Fe). 3+ The chelate, SQA-Fe 3+The absorption bands of NaYF4:Yb overlap, satisfying the conditions for the internal filtering effect. Simultaneously, SiO2 is used to coat NaYF4:Yb 3+ Er 3+ The surface can not only improve NaYF4:Yb 3+ Er 3+ The water solubility and biocompatibility of the probe prevent interference from the detection matrix on fluorescence, further enhancing the stability of the fluorescent probe. During detection, the strong oxidizing properties of H2O2 are used to decompose Fe... 2+ Oxidized to Fe 3+ SQA-Fe 3+ Due to the internal filtration effect, NaYF4:Yb 3+ Er 3+ Based on the principle of SiO2 fluorescence quenching, NaYF4:Yb was constructed. 3+ Er 3+ @SiO2-SQA-Fe 3+ Fluorescent nanosensors are used for the quantitative detection of H2O2 in milk.

[0044] The handheld liquid extractor 2 includes a liquid extraction cylinder and a liquid extraction tube head 3 fixedly mounted at the front end of the liquid extraction cylinder. The rear end of the test strip roll 1 can be detachably fitted onto the liquid extraction tube head 3. To avoid the difficulty in controlling the flow rate of the test liquid flowing through the test strip roll 1 when manually pushing and pulling with a conventional syringe, thus affecting the measurement results, in this embodiment, the liquid extraction cylinder is a syringe-like structure with a rear opening. The rear end of the liquid extraction cylinder is provided with a detachable rear cover 6. An electrically controlled push-pull device 5 that can extend and retract is fixedly mounted on the front side of the rear cover 6. The electrically controlled push-pull device 5 is located inside the liquid extraction cylinder, and a piston 4 is fixedly mounted at the front end of the electrically controlled push-pull device 5. The piston 4 slides and fills inside the liquid extraction cylinder. In this embodiment, the electrically controlled push-pull device 5 adopts an electric push rod structure.

[0045] An electrical control box 7 is fixedly installed on the rear side of the back cover 6. A mobile power supply is installed inside the electrical control box 7. A push-out switch 8 and a pull-back switch 9 are fixedly embedded on the rear surface of the electrical control box 7. The mobile power supply is connected in series with the push-out switch 8 and the pull-back switch 9 respectively. The push-out switch 8 and the pull-back switch 9 are respectively connected to the electrically controlled push-pull device 5. The push-out switch 8 and the pull-back switch 9 are used to control the push-out and pull-back actions of the electrically controlled push-pull device 5 respectively.

[0046] To further improve the stability of the test strip roll 1 on the liquid extraction tube head 3, such as Figure 5 As shown, a detachable strap 20 is fitted onto the liquid extraction tube head 3. The strap 20 is used to secure the rear end of the test strip roll 1 to the liquid extraction tube head 3. In this embodiment, the strap 20 is an elastic rubber band strap; in other embodiments, Velcro can be used instead.

[0047] Operating principle: Pour an appropriate amount of the test solution into a beaker. Tightly assemble the test strip roll 1 and the handheld liquid extractor 2 with the strap 20. Use the handheld liquid extractor 2 to extract the test sample, so that the test sample and the test strip roll 1 are in full contact and react. After the liquid has completely soaked the sample pad 17 and reacted, cut the test strip roll 1 along the cutting line 12 of the cutting area 11. Use a fluorescence signal detection instrument with a 980nm excitation light source to detect the fluorescence value of specific wavelengths at the T line and C line.

[0048] After the sample solution has soaked and fully reacted, when the sample solution at point 15 of the T-line coating contains the target H2O2, the strong oxidizing property of H2O2 will cause Fe... 2+ Oxidized to Fe 3+ SQA-Fe 3+ Due to the internal filtration effect, NaYF4:Yb 3+ Er 3+ @SiO2 fluorescence quenching: Under 980nm excitation light, the fluorescence value at coating 15 (T line) in negative results was significantly weaker than that of coating 16 (C line), while the fluorescence values ​​at coating 15 (T line) and coating 16 (C line) in positive results were basically consistent, both showing strong fluorescence. In other words, the fluorescence values ​​of the T line and C line in negative results were basically consistent, both showing strong fluorescence, while the fluorescence value of the T line in positive results was significantly weaker than that of the C line.

[0049] Meanwhile, the pH test pad at the end of the test strip can display the pH of the sample to be tested, and determine whether the test conditions are optimal.

[0050] Alternatively, the instrument for detecting this fluorescence value can be a portable fluorescence microplate reader with a 980 nm excitation light setting or a Hitachi F-4600 fluorescence spectrophotometer with a solid sample holder, depending on the actual situation.

[0051] Example 2: A portable, immersion-type test strip roll for detecting hydrogen peroxide in milk. The difference between this example and Example 1 is that the handheld applicator 2 in this example uses a medical handheld syringe from the prior art. Other results are the same as in Example 1.

[0052] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims and not by the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A portable, immersion-type test strip roll for detecting hydrogen peroxide in milk, characterized in that: Includes test strip rolls (1) and handheld liquid extractor (2); The test strip roll (1) is a roll-shaped structure that is open at both ends. The test strip roll (1) includes a backing layer (10) on its outer layer and a test layer (13) fixed inside the backing layer. The test layer (13) includes a C-line coating (15) and a T-line coating (16) arranged at intervals. The C-line coating (15) is located behind the T-line coating (16). The C-line coating (15) consists of a UCNPs coating and a fluorescent donor coating from the outside to the inside. The T-line coating (16) consists of a UCNPs coating, a fluorescent donor coating, and a fluorescent acceptor coating from the outside to the inside. The fluorescent donor coating uses NaYF4:Yb 3+ Er 3+ The coating is made of SiO2, and the fluorescent acceptor coating uses SQA-Fe. 3+ Coated; The handheld liquid extractor (2) includes a liquid extraction cylinder and a liquid extraction tube head (3) fixed at the front end of the liquid extraction cylinder. The rear end of the test paper roll (1) can be detachably mounted on the liquid extraction tube head (3).

2. The portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in claim 1, characterized in that: The backing layer (10) is made of a transparent and waterproof material.

3. The portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in claim 2, characterized in that: The backing layer (10) has a cutting area (11) that extends along the front-to-back direction. The test layer (13) is fixed inside the cutting area (11) and is broken at the cutting area (11).

4. The portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in claim 3, characterized in that: The inner side of the cutting area (11) protrudes from the inner wall of the backing layer (10) to form a strip structure extending in the vertical direction. The inner side of the strip structure is flush with the inner side of the test layer (13), and the end of the test layer (13) abuts against the left and right sides of the strip structure.

5. A portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in claim 3 or 4, characterized in that: A cutting line (12) is provided on the outer surface of the cutting area (11) along its central axis.

6. A portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in any one of claims 1-4, characterized in that: The test layer (13) includes a sample pad (17), a nitrocellulose pad (14), an absorbent pad (18) and a pH test pad (19) that are fixed in a roll shape inside the backing layer (10) from front to back. The rear end of the sample pad (17) and the front end of the absorbent pad (18) are respectively pressed onto the front and rear ends of the nitrocellulose pad (14). The C-line coating (15) and the T-line coating (16) are spaced apart on the inner side of the nitrocellulose pad (14).

7. A portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in claim 1, characterized in that: A detachable strap (20) is fitted on the liquid extraction tube head (3). The strap (20) is used to bind and fix the rear end of the test paper roll (1) to the liquid extraction tube head (3).

8. The portable, immersion-type test strip roll for detecting hydrogen peroxide in milk as described in claim 1, characterized in that: The liquid extraction cylinder is a syringe-like structure with a rear opening. The rear end of the liquid extraction cylinder is provided with a detachable rear cover (6). The front side of the rear cover (6) is fixed with an electrically controlled push-pull device (5) that can extend and retract back and forth. The electrically controlled push-pull device (5) is located inside the liquid extraction cylinder. The front end of the electrically controlled push-pull device (5) is fixed with a piston (4). The piston (4) slides and fills inside the liquid extraction cylinder. An electrical control box (7) is fixedly installed on the rear side of the back cover (6). A mobile power supply is installed inside the electrical control box (7). A push-out switch (8) and a pull-back switch (9) are fixedly embedded on the rear surface of the electrical control box (7). The mobile power supply is connected in series with the push-out switch (8) and the pull-back switch (9) respectively. The push-out switch (8) and the pull-back switch (9) are connected to the electric push-pull device (5) respectively. The push-out switch (8) and the pull-back switch (9) are used to control the push-out and pull-back actions of the electric push-pull device (5) respectively.

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