A multi-omics experimental device and experimental equipment

Abstract

The application discloses a multi-omics experiment device and experiment equipment. The multi-omics experiment device comprises a mounting seat, a pressing plate and a movable pressing block. The mounting seat is used for placing a microfluidic chip assembly. The pressing plate is used for covering the surface of the microfluidic chip assembly. The pressing plate is provided with a hollow structure. The hollow structure is arranged corresponding to a mark area of the microfluidic chip assembly. The movable pressing block is movably abutted against the upper surface of the pressing plate and is used for applying a pressing force to the pressing plate.

Description

Technical Field

[0001] This application relates to the field of space multi-omics experimental technology, and in particular to a multi-omics experimental device and equipment. Background Technology

[0002] Spatial multi-omics technology based on microfluidics uses a microfluidic chip with multiple lateral and longitudinal channels to add markers to molecules within cells on a tissue slide. The intersecting directions of the two labeling directions enable coordinate encoding of the spatial location of molecules within the cells. Typically, the labeling process involves placing the tissue to be labeled on a glass slide, attaching the slide to the microfluidic chip with channels, and adding different markers to each channel, allowing the markers to bind to the tissue, thereby adding coordinate information to the tissue to be labeled.

[0003] The above operation requires repeated addition of markers, thus consuming significant time and manpower. Related technologies utilize multi-omics experimental setups combined with robotic arms to automate marker addition, thereby improving operational efficiency. To ensure sufficient sealing between the flow channel chip and the glass slide, and to guarantee smooth flow of the markers within the channel under negative pressure, pressure is typically applied to the glass slide to ensure close contact with the flow channel chip. However, the area on the glass slide where the tissue to be labeled corresponds to the labeling area of ​​the flow channel chip. Furthermore, due to the dense and narrow flow channels in the labeling area of ​​the flow channel chip, there is a risk of channel blockage even under sealing conditions, affecting the labeling effect. Therefore, a solution is urgently needed that can both meet sealing requirements and ensure that the flow channels in the labeling area do not become blocked. Utility Model Content

[0004] To solve at least one of the above-mentioned technical problems, this application provides a multi-omics experimental device and equipment, and the technical solution adopted is as follows.

[0005] The multi-omics experimental apparatus provided in the first aspect of this application includes a mounting base, a pressure plate, and a movable pressure block. The mounting base is used to place a microfluidic chip assembly; the pressure plate is used to cover the surface of the microfluidic chip assembly, and the pressure plate has a clearance structure, which is set corresponding to the marking area of ​​the microfluidic chip assembly; the movable pressure block is movably abutted against the upper surface of the pressure plate and is used to apply a clamping force to the pressure plate.

[0006] In some embodiments of the first aspect of this application, the clearance structure includes a first clearance hole that penetrates both sides of the pressure plate, and at least a portion of the marking area of ​​the microfluidic chip assembly is exposed in the first clearance hole.

[0007] In some embodiments of the first aspect of this application, the marking area of ​​the microfluidic chip assembly is located in the projection area of ​​the first clearance hole in the microfluidic chip assembly.

[0008] In some embodiments of the first aspect of this application, the movable pressure block is provided with a second clearance hole, the second clearance hole penetrating both sides of the movable pressure block, and when the movable pressure block moves to the surface of the pressure plate, the second clearance hole can communicate with the first clearance hole.

[0009] In some embodiments of the first aspect of this application, the projections of the first clearance hole and the second clearance hole on the pressure plate coincide.

[0010] In some embodiments of the first aspect of this application, the microfluidic chip assembly includes an inlet region, a connecting region, and an outlet region connected in sequence, and a marking region of the microfluidic chip assembly is located in the connecting region; the movable pressure block is arranged in a strip shape, and the movable pressure block is at least correspondingly disposed in the connecting region, and the first clearance hole is disposed corresponding to the marking region.

[0011] In some embodiments of the first aspect of this application, the pressure plate is further provided with a reagent window, the reagent window is provided corresponding to the liquid inlet area of ​​the microfluidic chip assembly, and the reagent window is spaced apart from the first clearance hole; the multi-omics experimental device includes at least two of the movable pressure blocks, one of the movable pressure blocks is provided corresponding to the connection area, and the other movable pressure blocks are provided at least corresponding to the outer periphery of the reagent window of the microfluidic chip assembly.

[0012] In some embodiments of the first aspect of this application, the pressure plate is further provided with a negative pressure window, the negative pressure window is provided corresponding to the liquid outlet area of ​​the microfluidic chip assembly, and the negative pressure window is spaced apart from the first clearance hole; the multi-omics experimental device includes at least two of the movable pressure blocks, one of the movable pressure blocks is provided corresponding to the connection area, and the other movable pressure blocks are provided at least corresponding to the outer periphery of the negative pressure window of the microfluidic chip assembly.

[0013] In some embodiments of the first aspect of this application, when the movable pressure block moves to the surface of the pressure plate, the movable pressure block is configured to avoid the clearance structure.

[0014] In some embodiments of the first aspect of this application, the clearance structure includes a first clearance hole that penetrates both sides of the pressure plate. The minimum distance from the edge of the first clearance hole to the edge of the pressure plate is D. When the movable pressure block moves to the surface of the pressure plate, the length of the movable pressure block on the pressure plate is less than or equal to D.

[0015] In some embodiments of the first aspect of this application, the multi-omics experimental apparatus includes two movable pressure blocks, which are symmetrically arranged about the first clearance hole when they move to the surface of the pressure plate.

[0016] In some embodiments of the first aspect of this application, the multi-omics experimental apparatus further includes a base, the mounting base is disposed on the base, and the movable pressure block is rotatably connected to the base so that the movable pressure block rotates to the surface of the pressure plate or rotates away from the surface of the pressure plate.

[0017] In some embodiments of the first aspect of this application, the movable pressure block is a pneumatic pressure block.

[0018] In some embodiments of the first aspect of this application, the movable pressure block is an electric pressure block.

[0019] In some embodiments of the first aspect of this application, the multi-omics experimental device further includes a base, the mounting seat is detachably connected to the base, the mounting seat is provided with a first limiting part, the base is provided with a second limiting part, and when the mounting seat is disposed on the base, the first limiting part and the second limiting part are connected in cooperation.

[0020] In some embodiments of the first aspect of this application, the first limiting part is a limiting hole, and the second limiting part is a protruding post protruding from the surface of the mounting base, the protruding post being insertable into the limiting hole.

[0021] In some embodiments of the first aspect of this application, the mounting base is further provided with a third limiting part, the third limiting part protruding from the top surface of the mounting base, the protrusion height of the third limiting part being greater than that of the second limiting part, and when the mounting base is disposed on the base, the third limiting part is engaged with the outer peripheral side of the mounting base.

[0022] In some embodiments of the first aspect of this application, the pressure plate is provided with a heating structure.

[0023] In some embodiments of the first aspect of this application, the pressure plate is provided with a first magnetic connector, the first magnetic connector is disposed toward the mounting base, the mounting base is provided with a second magnetic connector, and when the pressure plate is disposed on the mounting base, the first magnetic connector and the second magnetic connector are connected to conduct the heating structure.

[0024] In some embodiments of the first aspect of this application, the pressure plate is further provided with a temperature sensor.

[0025] In some embodiments of the first aspect of this application, the surface of the pressure plate used to cover the microfluidic chip assembly is further provided with a sealing gasket, the sealing gasket being sealed between the pressure plate and the microfluidic chip assembly.

[0026] In some embodiments of the first aspect of this application, the pressure plate is provided with a first positioning structure, the mounting base is provided with a second positioning structure, and when the pressure plate covers the microfluidic chip assembly, the first positioning structure and the second positioning structure are aligned and connected.

[0027] In some embodiments of the first aspect of this application, the first positioning structure includes a positioning notch, and the second positioning structure includes a positioning protrusion. When the pressure plate is closed onto the microfluidic chip assembly, the positioning protrusion extends into the positioning notch.

[0028] In some embodiments of the first aspect of this application, the surface of the pressure plate facing the microfluidic chip assembly is provided with a negative pressure groove, the bottom of the negative pressure groove is provided with a negative pressure window, the negative pressure window penetrates the surface of the pressure plate, the negative pressure groove is provided corresponding to the liquid outlet area of ​​the microfluidic chip assembly, and the negative pressure window is spaced apart from the first clearance hole provided on the pressure plate.

[0029] In some embodiments of the first aspect of this application, the multi-omics experimental apparatus further includes an ejection assembly, the ejection assembly including a first lifting member that can be lifted vertically, the first lifting member being disposed outside the mounting base, the first lifting member being used to lift the glass plate of the microfluidic chip assembly to separate it from the flow channel chip of the microfluidic chip assembly.

[0030] In some embodiments of the first aspect of this application, the ejection assembly further includes a second lifting member that can be ejected in a horizontal direction. The second lifting member is disposed on the outside of the mounting base, and the second lifting member is disposed on the same side or different side from the first lifting member. The second lifting member is used to abut against the side of the flow channel chip of the microfluidic chip assembly during ejection, so as to fix the flow channel chip in the mounting base.

[0031] In some embodiments of the first aspect of this application, the ejection assembly further includes an adsorption member, which is at least disposed on the bottom or side surface of the mounting base, and is used to adsorb and fix the flow channel chip of the microfluidic chip assembly.

[0032] In some embodiments of the first aspect of this application, the multi-omics experimental apparatus further includes a microfluidic chip assembly, the microfluidic chip assembly including a glass sheet and a flow channel chip, and at least one side edge of the glass sheet is provided with a protrusion, the protrusion protruding from the side of the flow channel chip when the glass sheet covers the upper surface of the flow channel chip.

[0033] When the first lifting member is raised, it abuts against the protrusion to drive the glass sheet to separate from the flow channel chip.

[0034] In some embodiments of the first aspect of this application, the surface of the mounting base is further provided with a positioning groove for placing the microfluidic chip assembly.

[0035] In some embodiments of the first aspect of this application, the multi-omics experimental apparatus further includes a base, the mounting base is disposed on the base, and the base is provided with a waste liquid collection tank and an outlet communicating with the waste liquid collection tank.

[0036] The second aspect of this application provides an experimental device, including a negative pressure module and the multi-omics experimental apparatus provided in the first aspect. The negative pressure module is movably connected to the negative pressure window of the multi-omics experimental apparatus and is used to perform negative pressure suction on the flow channels in the microfluidic chip assembly.

[0037] In some embodiments of the second aspect of this application, the negative pressure module includes a negative pressure suction cup, which is movably connected to the negative pressure window of the multi-omics experimental device, and the negative pressure suction cup is sealed to the negative pressure window.

[0038] In some embodiments of the second aspect of this application, the negative pressure module includes a base and a cantilever that are rotatably connected to each other, the negative pressure suction cup is disposed on the cantilever, and the cantilever is used to drive the negative pressure suction cup to move to connect with the negative pressure window, or to drive the negative pressure suction cup to move out of the negative pressure window.

[0039] In some embodiments of the second aspect of this application, the experimental equipment further includes an insulated box and a heating module, wherein the multi-omics experimental device, the negative pressure module and the heating module are disposed in the insulated box, and the heating module is used to provide heat to the insulated box.

[0040] In some embodiments of the second aspect of this application, the heating module includes a heating element and a heat dissipation structure, wherein the heat dissipation structure is connected to the heating element.

[0041] In some embodiments of the second aspect of this application, the negative pressure module includes a negative pressure suction cup and a base and a cantilever that are rotatably connected to each other. The negative pressure suction cup is disposed on the cantilever, and the heating module is disposed on the base, with the heating module avoiding the cantilever.

[0042] In some embodiments of the second aspect of this application, the insulated box includes a box body and a lid, the box body being used to house the multi-omics experimental device, the negative pressure module and the heating module, the box body having an opening, and the lid being able to seal or open the opening.

[0043] In some embodiments of the second aspect of this application, the opening is located at the top of the housing, and the cover is foldable to one side of the opening to open the opening, or the cover is unfoldable to seal the opening.

[0044] In some embodiments of the second aspect of this application, the insulated box further includes a linear module disposed at the opening, the cover being connected to the linear module, and the linear module being used to drive the cover to fold or unfold along the extension direction of the linear module.

[0045] The embodiments of this application have at least the following beneficial effects: Through the combined action of the pressure plate and the movable pressure block, on the one hand, the clamping force of the movable pressure block on the pressure plate is used to press the glass plate and the flow channel chip in the microfluidic chip assembly together, ensuring a close fit and giving the microfluidic chip assembly good sealing performance, facilitating the flow of the reaction reagent (label) in the flow channel under negative pressure. On the other hand, the pressure plate can evenly distribute the clamping force generated by the movable pressure block, ensuring that all parts of the surface of the microfluidic chip assembly are pressed, improving the stability and uniformity of the pressure on the microfluidic chip assembly. By setting a void structure on the pressure plate, the marking area of ​​the microfluidic chip assembly can be avoided, thus preventing direct compression of the marking area. Instead, the compression force is applied to the outer periphery of the marking area, ensuring the sealing performance of the microfluidic chip assembly and overcoming the problem of flow channel blockage caused by direct compression of the marking area, thereby improving the marking effect. Attached Figure Description

[0046] The aspects and advantages described and / or added to the embodiments of this application will become apparent and readily understood in conjunction with the following drawings. It should be noted that the embodiments illustrated in the following drawings are exemplary and are used only to explain this application, and should not be construed as limiting this application.

[0047] Figure 1 This is a schematic diagram of the structure of the multi-omics experimental device provided in the embodiments of this application;

[0048] Figure 2 This is an exploded view of the structure of the multi-omics experimental device provided in the embodiments of this application;

[0049] Figure 3 A schematic diagram of the structure of a multi-omics experimental device provided in an embodiment of this application, in one usage state.

[0050] Figure 4 A schematic diagram of the structure of the multi-omics experimental device provided in an embodiment of this application under another usage state;

[0051] Figure 5 A schematic diagram of the pressure plate of the multi-omics experimental device provided in the embodiments of this application;

[0052] Figure 6 A schematic diagram of the mounting base for the multi-omics experimental device provided in the embodiments of this application;

[0053] Figure 7 A schematic diagram of the base of the multi-omics experimental device provided in the embodiments of this application;

[0054] Figure 8 A schematic diagram of the microfluidic chip assembly structure of the multi-omics experimental device provided in the embodiments of this application;

[0055] Figure 9 This is a schematic diagram of the structure of the experimental equipment provided in the embodiments of this application;

[0056] Figure 10 A schematic diagram showing the negative pressure module of the experimental equipment provided in the embodiments of this application in a separated state from the multi-omics experimental device;

[0057] Figure 11 A schematic diagram showing the connection state of the negative pressure module of the experimental equipment provided in the embodiments of this application with the multi-omics experimental device;

[0058] Figure 12 A flowchart of a spatial multi-omics experimental method is provided as an example of an embodiment of this application;

[0059] Figure 13 A flowchart of a spatial multi-omics experimental method provided as an example of an embodiment of this application.

[0060] Figure label: 100, multi-omics experimental setup;

[0061] 10. Mounting base; 11. Second magnetic connector; 12. Second positioning structure; 13. Positioning groove; 14. First limiting part;

[0062] 20. Pressure plate; 21. First clearance hole; 22. Reagent window; 23. Negative pressure window; 24. Heating structure; 25. First magnetic connector; 26. Temperature sensor; 27. First positioning structure; 28. Negative pressure groove;

[0063] 30. Movable pressure block; 31. Second clearance hole;

[0064] 40. Base; 41. Waste liquid collection tank; 42. Second limiting part; 43. Third limiting part;

[0065] 51. First lifting component; 52. Second lifting component;

[0066] 200. Microfluidic chip assembly; 201. Flow channel chip; 202. Glass slide; 2021. Protrusion; 203. Marking area; 204. Liquid inlet area; 205. Liquid outlet area; 206. Connecting area;

[0067] 300. Experimental equipment; 310. Negative pressure module; 311. Negative pressure suction cup; 312. Base; 313. Cantilever;

[0068] 320. Insulated box; 321. Box body; 3211. Opening; 322. Lid; 323. Linear module;

[0069] 330. Heating module; 331. Heating element; 332. Heat dissipation structure. Detailed Implementation

[0070] The embodiments of this application are described in detail below with reference to the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0071] In the description of this application, it should be understood that the terms "center", "middle", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0072] In the description of this application, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0073] In the description of this application, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0074] In the description of this application, the use of terms such as "as one implementation," "an embodiment," "some examples," "some embodiments," "illustrative embodiment," "example," "specific example," "some examples," etc., indicates that the specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0075] Firstly, please refer to Figures 1 to 4 This application provides a multi-omics experimental device 100, including a mounting base 10, a pressure plate 20, and a movable pressure block 30. The mounting base 10 is used to place a microfluidic chip assembly 200, and the pressure plate 20 is used to cover the surface of the microfluidic chip assembly 200. The pressure plate 20 is provided with a clearance structure, which is set corresponding to the marking area 203 of the microfluidic chip assembly 200. The movable pressure block 30 is movably abutted against the upper surface of the pressure plate 20 and is used to apply a clamping force to the pressure plate 20. Through the cooperation of the pressure plate 20 and the movable pressure block 30, the clamping force of the movable pressure block 30 on the pressure plate 20 is used to press the glass plate 202 and the flow channel chip 201 in the microfluidic chip assembly 200 together, ensuring that the two are closely fitted. This gives the microfluidic chip assembly 200 good sealing performance, which facilitates the flow of reaction reagents (labels) in the flow channel under negative pressure. On the other hand, the pressure plate 20 can evenly distribute the clamping force generated by the movable pressure block 30, so that all parts of the surface of the microfluidic chip assembly 200 can be pressed, improving the stability and uniformity of the pressure on the microfluidic chip assembly 200. By setting an air-proof structure in the pressure plate 20, it is convenient to observe the marking area 203 under a microscope. This avoids direct compression of the marking area 203 of the microfluidic chip assembly 200, and instead applies the compression force to the outer periphery of the marking area 203. This ensures the sealing performance of the microfluidic chip assembly 200 and overcomes the problem of flow channel blockage caused by direct compression of the marking area 203, thus improving the marking effect.

[0076] In some embodiments, the clearance structure includes a first clearance hole 21 that penetrates both sides of the pressure plate 20, and at least a portion of the marking area 203 of the microfluidic chip assembly 200 is exposed through the first clearance hole 21. By providing the first clearance hole 21, the area above the marking area 203 can be left open, thereby preventing the pressure plate 20 from directly pressing the marking area 203, achieving the effect that the pressure plate 20 can both press down on the microfluidic chip assembly 200 and avoid the marking area 203.

[0077] Optionally, the marking area 203 of the microfluidic chip assembly 200 may be entirely or partially located in the first clearance hole 21. That is, when the marking area 203 is entirely located in the first clearance hole 21, the marking area 203 is entirely located in the projection area of ​​the first clearance hole 21 in the microfluidic chip assembly 200. Of course, in other examples, a portion of the marking area 203 may also be located outside the projection area of ​​the first clearance hole 21.

[0078] As an alternative implementation, in addition to providing the first clearance hole 21, the clearance structure can also be provided as a clearance groove. That is, the clearance groove does not penetrate through both sides of the pressure plate 20, but is provided as a blind hole. For example, a clearance groove can be provided on the bottom surface of the pressure plate 20 (the surface facing the microfluidic chip assembly 200), and the position of the clearance groove corresponds to the marking area 203. In this way, the position of the clearance groove can also avoid contact with the marking area 203, thereby achieving the effect of preventing the pressure plate 20 from pressing the marking area 203.

[0079] Optionally, the movable pressure block 30 can be a plate-shaped, block-shaped, or strip-shaped structure. Taking the strip shape as an example, it can be a long strip or a short strip. The following will describe the different implementation methods of the movable pressure block 30.

[0080] In the first example, such as Figure 1 As shown, the movable pressure block 30 can be elongated to increase the pressure area of ​​the movable pressure block 30 on the pressure plate 20, thus achieving a better pressing effect. However, with this configuration, when the movable pressure block 30 presses against the surface of the pressure plate 20, it will cover the first clearance hole 21. Therefore, in some embodiments, the movable pressure block 30 is provided with a second clearance hole 31, which penetrates both sides of the movable pressure block 30. When the movable pressure block 30 moves to the surface of the pressure plate 20, the second clearance hole 31 can connect with the first clearance hole 21. Therefore, by providing the second clearance hole 31 and connecting it with the first clearance hole 21, the first clearance hole 21 can be exposed again. This avoids the marking area 203 being squeezed by the movable pressure block 30, and also exposes the marking area 203 through the second clearance hole 31 and the first clearance hole 21, facilitating observation of the marking area 203.

[0081] In some embodiments, the projections of the first clearance hole 21 and the second clearance hole 31 on the pressure plate 20 coincide. In this way, the second clearance hole 31 can fully expose the first clearance hole 21, preventing the first clearance hole 21 from being covered, and also preventing the field of view from being obstructed when observing the marking area 203.

[0082] In some embodiments, the microfluidic chip assembly 200 includes an inlet region 204, a connecting region 206, and an outlet region 205 connected in sequence. A marking region 203 of the microfluidic chip assembly 200 is located in the connecting region 206. A movable pressure block 30 is strip-shaped and is at least correspondingly disposed in the connecting region 206, with a first clearance hole 21 corresponding to the marking region 203. The strip-shaped movable pressure block 30 can better cover the connecting region 206 and helps to apply sufficient pressure to the pressure plate 20, achieving a sealing effect for the microfluidic chip assembly 200. Since the inlet region 204 and the outlet region 205 are located on both sides of the connecting region 206, limiting the pressure application position of the movable pressure block 30 to the connecting region 206 also prevents the movable pressure block 30 from pressing against the inlet region 204 or the outlet region 205, thus preventing the movable pressure block 30 from affecting the addition of reaction reagents (markers) in the inlet region 204 or the negative pressure suction operation in the outlet region 205.

[0083] In some embodiments, the pressure plate 20 is further provided with a reagent window 22, which is disposed corresponding to the liquid inlet area 204 of the microfluidic chip assembly 200, and the reagent window 22 is spaced apart from the first clearance hole 21. The multi-omics experimental device 100 includes at least two movable pressure blocks 30, one of which is disposed corresponding to the connection area 206, and the other movable pressure blocks 30 are disposed at least around the outer periphery of the reagent window 22 of the microfluidic chip assembly 200. The reagent window 22 exposes the liquid inlet area 204, facilitating the addition of reaction reagents (labels) to the microfluidic chip assembly 200. In addition to applying pressure to the connection area 206, one movable pressure block 30 also applies pressure to the liquid inlet area 204 simultaneously, which makes the pressure of the pressure plate 20 on the microfluidic chip assembly 200 more uniform and improves the pressing effect. In addition, another movable pressure block 30 is pressed against the outer periphery of the reagent window 22, which can prevent the reagent window 22 from being blocked and avoid affecting the addition of reaction reagents.

[0084] In some embodiments, please refer to Figure 5The pressure plate 20 is also provided with a negative pressure window 23, which is set corresponding to the liquid outlet area 205 of the microfluidic chip assembly 200, and the negative pressure window 23 is spaced apart from the first clearance hole 21. The multi-omics experimental device 100 includes at least two movable pressure blocks 30, one of which is set corresponding to the connection area 206, and the other movable pressure blocks 30 are set at least on the outer periphery of the negative pressure window 23 of the microfluidic chip assembly 200. The negative pressure window 23 can expose the liquid outlet area 205, so as to apply negative pressure to the flow channel in the microfluidic chip assembly 200, guide the marker to flow in the flow channel and fully combine with cell tissue. In addition to applying pressure to the connection area 206, one movable pressure block 30 also applies pressure to the liquid outlet area 205 at the same time, which can make the pressure of the pressure plate 20 on the microfluidic chip assembly 200 more uniform and improve the pressing effect. In addition, another movable pressure block 30 is pressed against the outer periphery of the negative pressure window 23, which can prevent the negative pressure window 23 from being blocked and avoid affecting the operation of negative pressure suction.

[0085] For example, one, two, three or more movable pressure blocks 30 can be provided. When one movable pressure block 30 is provided, it can press against the connecting area 206. When two movable pressure blocks 30 are provided, in addition to the connecting area 206, the other movable pressure block 30 can be provided in either the liquid inlet area 204 or the liquid outlet area 205. Of course, movable pressure blocks 30 can also be provided simultaneously in the liquid inlet area 204, the liquid outlet area 205, and the connecting area 206 to make the force on the pressure plate 20 more even.

[0086] In the second example, such as Figure 9 As shown, the movable pressure block 30 can be configured as a short strip, that is, the length of the movable pressure block 30 is shorter than that in the previous embodiment. Specifically, when the movable pressure block 30 moves to the surface of the pressure plate 20, the movable pressure block 30 is configured to avoid the clearance structure. In this way, the movable pressure block 30 can avoid squeezing the clearance structure part of the pressure plate 20, thereby further preventing the clearance structure from squeezing the marking area 203.

[0087] In some embodiments, the clearance structure includes a first clearance hole 21 that penetrates both sides of the pressure plate 20. The minimum distance from the edge of the first clearance hole 21 to the edge of the pressure plate 20 is D. When the movable pressure block 30 moves to the surface of the pressure plate 20, the length of the movable pressure block 30 on the pressure plate 20 is less than or equal to D. Thus, when the clearance structure is set in the form of the first clearance hole 21, the movable pressure block 30 can avoid obstructing the first clearance hole 21, ensuring that the first clearance hole 21 can expose the marking area 203.

[0088] In some embodiments, the multi-omics experimental apparatus 100 includes two movable pressure blocks 30. When the movable pressure blocks 30 move to the surface of the pressure plate 20, the two movable pressure blocks 30 are symmetrically arranged about the first clearance hole 21. By setting two movable pressure blocks 30 and symmetrically arranging them about the first clearance hole 21, the movable pressure blocks 30 can apply pressure evenly to both sides of the pressure plate 20, making the force on the entire pressure plate 20 more uniform.

[0089] In some embodiments, the multi-omics experimental apparatus 100 further includes a base 40, a mounting base 10 disposed on the base 40, and a movable pressure block 30 rotatably and vertically connected to the base 40, so that the movable pressure block 30 rotates down to the surface of the pressure plate 20, or rotates up to leave the surface of the pressure plate 20. The movable pressure block 30's rotational and lifting mechanism for entering or leaving above the pressure plate 20 shortens its movement path and simplifies its movement, making its movement easier to achieve.

[0090] For example, the movable pressure block 30 can be a pneumatic pressure block or an electric pressure block. That is, the rotation and pressing action can be achieved by pneumatic or electric drive, thereby applying pressure to the pressure plate 20. When releasing the pressure, the movable pressure block 30 can also be rotated upward by pneumatic or electric drive, at which time the pressure of the movable pressure block 30 on the pressure plate 20 disappears.

[0091] In some embodiments, please refer to Figure 6 and Figure 7 The mounting base 10 is detachably connected to the base 40. The mounting base 10 is provided with a first limiting part 14, and the base 40 is provided with a second limiting part 42. When the mounting base 10 is placed on the base 40, the first limiting part 14 and the second limiting part 42 cooperate to connect. By utilizing the cooperation of the first limiting part 14 and the second limiting part 42, the mounting base 10 can be accurately placed on the base 40, improving the placement accuracy of the mounting base 10, preventing misalignment between the mounting base 10 and the base 40 after the pressure plate 20 is pressed, and improving the reliability of the experimental process.

[0092] For example, the first limiting part 14 is a limiting hole, and the second limiting part 42 is a protruding post protruding from the surface of the mounting base 10, which can be inserted into the limiting hole. By using the insertion and cooperation of the protruding post and the limiting hole, the limiting effect of the mounting base 10 can be achieved. By making the first limiting part 14 into the form of a hole, it is easier to lay the mounting base 10 flat when it is removed.

[0093] In some embodiments, the mounting base further includes a third limiting portion 43, which protrudes from the top surface of the mounting base 10. The protrusion height of the third limiting portion 43 is greater than that of the second limiting portion 42. The third limiting portion 43 is used to limit the pressure plate 20. The third limiting portion 43 helps to guide the pressure plate 20 to accurately cover the mounting base 10, thereby achieving the limiting of the pressure plate 20.

[0094] Since the binding of the marker to the cells / tissue requires a certain temperature, heating can improve the labeling effect. Therefore, in some embodiments, please refer to [the relevant documentation]. Figure 5 The pressure plate 20 is equipped with a heating structure 24, which provides suitable temperature conditions for the microfluidic chip assembly 200, helping to improve experimental efficiency and results. For example, the heating structure 24 can be a PTC (Positive Temperature Coefficient) thermostat embedded inside the pressure plate 20. For instance, the pressure plate 20 may have a sandwich structure or cavity to accommodate the PTC thermostat.

[0095] In some embodiments, the pressure plate 20 is provided with a first magnetic connector 25, which is positioned towards the mounting base 10. The mounting base 10 is provided with a second magnetic connector 11. When the pressure plate 20 is positioned on the mounting base 10, the first magnetic connector 25 and the second magnetic connector 11 are connected to conduct electricity to the heating structure 24. Utilizing magnetic attraction to connect or disconnect the heating structure 24 from the power supply simplifies the operation of the heating structure 24 and improves the convenience of experimental operations.

[0096] In some embodiments, the pressure plate 20 is further provided with a temperature sensor 26. The temperature sensor 26 can monitor and provide feedback on the heating level of the pressure plate 20, and can also adjust the heating power to ensure that the microfluidic chip assembly 200 is under suitable reaction conditions. The heating structure 24, in conjunction with the temperature sensor 26, can achieve temperature control, for example, controlling the temperature of the pressure plate 20 and the microfluidic chip assembly 200 at around 37°C. This ensures that the cell tissue in the glass slide 202 and the markers in the flow channels maintain a high binding efficiency, which is typically highest at 37°C.

[0097] In some embodiments, the surface of the pressure plate 20 that covers the microfluidic chip assembly 200 is further provided with a sealing gasket (not shown), which seals between the pressure plate 20 and the microfluidic chip assembly 200. By providing the sealing gasket, the sealing performance between the pressure plate 20 and the microfluidic chip assembly 200 can be further improved, which helps to improve the effect of negative pressure suction from the negative pressure window 23.

[0098] In some embodiments, please refer to Figure 2and Figure 3 The pressure plate 20 is provided with a first positioning structure 27, and the mounting base 10 is provided with a second positioning structure 12. When the pressure plate 20 is closed on the microfluidic chip assembly 200, the first positioning structure 27 and the second positioning structure 12 are aligned and connected. The cooperation between the first positioning structure 27 and the second positioning structure 12 helps to improve the accuracy of the placement of the pressure plate 20.

[0099] For example, the first positioning structure 27 includes a positioning notch, and the second positioning structure 12 includes a positioning protrusion. When the pressure plate 20 covers the microfluidic chip assembly 200, the positioning protrusion extends into the positioning notch. The first positioning structure 27 and the second positioning structure 12 are connected in the form of a positioning protrusion and a positioning notch, making the positioning structure easy to implement.

[0100] In some embodiments, the surface of the pressure plate 20 facing the microfluidic chip assembly 200 is provided with a negative pressure groove 28, and a negative pressure window 23 is provided at the bottom of the negative pressure groove 28. The negative pressure window 23 penetrates the surface of the pressure plate 20. The negative pressure groove 28 is provided corresponding to the liquid outlet area 205 of the microfluidic chip assembly 200, and the negative pressure window 23 is spaced apart from the first clearance hole 21 provided on the pressure plate 20. The negative pressure groove 28 can create a negative pressure space between the surface of the microfluidic chip assembly 200 and the pressure plate 20, ensuring the effect of negative pressure attraction. With this arrangement, the lower surface of the pressure plate 20 can be prevented from directly contacting the glass slide 202 of the liquid outlet area 205, which helps to avoid contamination of the pressure plate 20 by the labeled reagent.

[0101] In some embodiments, the multi-omics experimental apparatus 100 further includes an ejection assembly, which includes a first lifting member 51 that can be lifted vertically. The first lifting member 51 is disposed on the outside of the mounting base 10 and is used to lift the glass plate 202 of the microfluidic chip assembly 200 to separate it from the flow channel chip 201 of the microfluidic chip assembly 200. By setting the ejection assembly, sufficient force can be provided to separate the glass plate 202 from the flow channel chip 201, avoiding manual operation and improving the convenience and reliability of the operation of peeling off the glass plate 202. Exemplarily, the first lifting member 51 can be a power structure such as a linear motor or a cylinder.

[0102] In some embodiments, the ejection assembly further includes a second lifting member 52 that can be ejected horizontally. The second lifting member 52 is disposed on the outside of the mounting base 10, and may be disposed on the same side or different side from the first lifting member 51. The second lifting member is used to abut against the side of the flow channel chip 201 of the microfluidic chip assembly 200 during ejection to fix the flow channel chip 201 in the mounting base 10. By utilizing the abutment action of the second lifting member 52 against the flow channel chip 201, the flow channel chip 201 can be fixed, preventing the flow channel chip 201 from being lifted when the glass plate 202 is removed. Therefore, the separation of the glass plate 202 and the flow channel chip 201 can be achieved.

[0103] As an alternative implementation, the ejection assembly also includes an adsorption element, which is at least disposed on the bottom or side surface of the mounting base 10. The adsorption element is used to adsorb and fix the flow channel chip 201 of the microfluidic chip assembly 200. The adsorption element can also fix the flow channel chip 201, thereby achieving separation of the glass sheet 202 and the flow channel chip 201 under the lifting force of the first lifting element 51.

[0104] In some embodiments, please refer to Figure 8 The multi-omics experimental device 100 also includes a microfluidic chip assembly 200, which includes a glass sheet 202 and a flow channel chip 201. At least one edge of the glass sheet 202 has a protrusion 2021. When the glass sheet 202 covers the upper surface of the flow channel chip 201, the protrusion 2021 protrudes from the side of the flow channel chip 201. When the first lifting member 51 is lifted, it abuts against the protrusion 2021 to separate the glass sheet 202 from the flow channel chip 201. Because the glass sheet 202 and the flow channel chip 201 have a sealing effect after they are covered, that is, a certain surface tension keeps the glass sheet 202 and the flow channel chip 201 in close contact, which makes it difficult for the glass sheet 202 to separate from the flow channel chip 201. Therefore, by providing the protrusion 2021, when it is necessary to separate the glass sheet 202 from the flow channel chip 201, the glass sheet 202 can be lifted by applying force from the protrusion 2021 so as to separate it from the flow channel chip 201.

[0105] In some embodiments, the surface of the mounting base 10 is further provided with a positioning groove 13 for placing the microfluidic chip assembly 200. The positioning groove 13 serves to fix and limit the microfluidic chip assembly 200, preventing it from shifting or falling out of the mounting base 10. Exemplarily, the channel chip 201 also has a soft, adhesive characteristic; therefore, by placing the microfluidic chip assembly 200 in the mounting base 10, the mounting base 10 can be fixed in place. When picking up or placing the microfluidic chip assembly 200, the mounting base 10 can be operated (e.g., using a robotic arm or operator to hold the mounting base 10), avoiding direct contact with the channel chip 201 and improving the convenience and reliability of the picking and placing operation.

[0106] In some embodiments, the base 40 is provided with a waste liquid collection tank 41 and an outlet connected to the waste liquid collection tank 41. The waste liquid collection tank 41 can collect the reaction reagents that overflow from the microfluidic chip assembly 200 under negative pressure, thereby avoiding contamination of areas outside the multi-omics experimental device 100. After the experiment, the waste liquid in the waste liquid collection tank 41 can be collected and cleaned from the outlet.

[0107] Secondly, please refer to Figures 9 to 11 This application also provides an experimental device 300, including a negative pressure module 310 and the multi-omics experimental device 100 provided in the first aspect. The negative pressure module 310 is movably connected to the negative pressure window 23 of the multi-omics experimental device 100. The negative pressure module 310 is used to apply negative pressure to the flow channels in the microfluidic chip assembly 200. Utilizing the negative pressure effect of the negative pressure module 310, it can provide power to the liquid in the microfluidic chip assembly 200, solving the problem of discontinuous liquid flow in the flow channels of the flow channel chip 201 due to temperature changes, improving the binding effect of the label to cell tissues, and increasing the labeling efficiency. By movably connecting the negative pressure module 310 to the multi-omics experimental device 100, when the multi-omics experimental device 100 is adding liquid or removing / placing the microfluidic chip assembly 200, the negative pressure module 310 can be removed (e.g., when adding liquid or removing / placing the microfluidic chip assembly 200). Figure 11 As shown), the negative pressure module 310 is moved to connect with the negative pressure window 23 of the multi-omics experimental device 100 only when negative pressure suction is required (as shown). Figure 10 As shown in the figure, this helps to improve the ease of operation and practicality of the experimental equipment 300.

[0108] In some embodiments, the negative pressure module 310 includes a negative pressure suction cup 311, which is movably connected to the negative pressure window 23 of the multi-omics experimental device, and the negative pressure suction cup 311 is sealed to the negative pressure window 23. The negative pressure suction cup 311 can form a sealed connection with the negative pressure window 23 to ensure sealing performance during the negative pressure suction process.

[0109] In some embodiments, the negative pressure module 310 includes a base 312 and a cantilever 313 rotatably connected to each other. A negative pressure suction cup 311 is disposed on the cantilever 313, which is used to move the negative pressure suction cup 311 to connect with the negative pressure window 23, or to move the negative pressure suction cup 311 out of the negative pressure window 23. By installing a drive module in the base 312, the base 312 can provide power for the rotation of the cantilever 313, and the cantilever 313 can drive the negative pressure suction cup 311 to move. When the negative pressure suction cup 311 is not in use, the cantilever 313 can drive the negative pressure suction cup 311 to rotate away from the multi-omics experimental device 100 by a certain distance, avoiding interference with other experimental operations of the multi-omics experimental device 100. When the negative pressure suction cup 311 is in use, the cantilever 313 can be rotated to connect with the multi-omics experimental device 100, improving the convenience and automation of experimental operations.

[0110] Since the binding of the marker to the cells / tissues requires a certain temperature, heating helps improve the labeling effect. Therefore, in some embodiments, the experimental apparatus 300 also includes an incubator 320 and a heating module 330. The multi-omics experimental device 100, the negative pressure module 310, and the heating module 330 are disposed in the incubator 320, and the heating module 330 is used to provide heat to the incubator 320. By utilizing the combined action of the heating module 330 and the incubator 320, the incubator 320 can be maintained at a suitable temperature under the heating effect of the heating module 330, thereby providing a suitable environment for the binding of the cells / tissues to the marker, which helps improve experimental efficiency and results.

[0111] In some embodiments, the heating module 330 includes a heating element 331 and a heat dissipation structure 332, with the heat dissipation structure 332 connected to the heating element 331. By providing the heat dissipation structure 332, the heat generated by the heating element 331 can be diffused, thereby increasing the heating rate of the insulation box 320 and helping to make the temperature in the insulation box 320 more uniform. Exemplarily, the heating element 331 may be a heating chip, etc., and the heat dissipation structure 332 may be a cooling fan, heat dissipation fins, etc.

[0112] In some embodiments, the heating module 330 is disposed on the base 312, and the heating module 330 is disposed to avoid the cantilever 313. By disposing the heating module 330 in the base 312, it is helpful to integrate the heating module 330 and the negative pressure module 310 together, making the structure of the experimental device 300 more compact and reducing the space occupied in the housing 321. The fact that the heating module 330 is disposed to avoid the cantilever 313 can prevent the heating module 330 from affecting the rotation of the cantilever 313. Exemplarily, the cantilever 313 can be rotatably disposed on the top of the base 312, and the heating module 330 can be fixedly disposed on the side of the base 312, so that the cantilever 313 can operate without affecting the heating module 330. The heat dissipation direction of the heat dissipation structure 332 can be the exhaust direction of the cooling fan or the extension direction of the heat dissipation fins.

[0113] In some embodiments, the insulated box 320 includes a box body 321 and a lid 322. The box body 321 is used to house the multi-omics experimental device 100, the negative pressure module 310, and the heating module 330. The box body 321 has an opening 3211, and the lid 322 can close or open the opening 3211. Utilizing the opening and closing function of the lid 322 on the opening 3211, when the lid 322 is open, it is convenient to put the microfluidic chip assembly 200 into the box body 321 and to add liquid to it. When the lid 322 is closed, it can prevent heat dissipation within the space of the box body 321, thus providing a good heat preservation effect within the box body 321.

[0114] In some embodiments, the opening 3211 is located at the top of the housing 321, and the cover 322 is foldable to one side of the opening 3211 to open the opening 3211, or the cover 322 can be unfolded to close the opening 3211. By setting the cover 322 to a foldable opening method, the space occupied by the cover 322 when it is open can be reduced, thereby saving space in the housing 321.

[0115] In some embodiments, the insulated box 320 further includes a linear module 323 disposed at the opening 3211, with the lid 322 connected to the linear module 323. The linear module 323 is used to drive the lid 322 to fold or unfold along the extension direction of the linear module 323. The linear module 323 can guide the folding or unfolding of the lid 322, making it easy to open or close the lid 322. The linear module 323 may include a linear motor to electrically drive the lid 322, and the linear module 323 may also include a linear track to guide the movement of the lid 322.

[0116] Thirdly, please refer to Figure 12 This application also provides a spatial multi-omics experimental method, including:

[0117] S100. Install the microfluidic chip assembly 200 into the mounting base 10;

[0118] S200. Cover the surface of the microfluidic chip assembly 200 with the pressure plate 20 so that the void structure corresponds to the marking area 203 of the microfluidic chip assembly 200;

[0119] S300. The movable pressure block 30 moves to the surface of the pressure plate 20 and applies a clamping force to the pressure plate 20;

[0120] S400. Add reaction reagents to the microfluidic chip assembly 200 to initiate a reaction;

[0121] S500. Remove the movable pressure block 30 from the surface of the pressure plate 20;

[0122] S600. Remove the pressure plate 20;

[0123] S700. Remove the microfluidic chip assembly 200.

[0124] By utilizing the combined pressing action of the movable pressure block 30 and the pressure plate 20, a sealed connection can be achieved between the flow channel chip 201 in the microfluidic chip assembly 200 and the glass slide 202 with the tissue to be labeled, ensuring reliable sealing. The air-proof structure on the pressure plate 20 prevents direct pressure from the pressure plate 20 on the labeling area 203 of the microfluidic chip assembly 200, thereby preventing flow channel blockage and improving the labeling effect. The above experimental method enables the labeling of cell tissues.

[0125] In some embodiments, the clearance structure includes a first clearance hole 21, and the movable pressure block 30 is provided with a second clearance hole 31. The above step S300, in which the movable pressure block 30 moves to the surface of the pressure plate 20 and applies a clamping force to the pressure plate 20, includes:

[0126] S310. The movable pressure block 30 moves to the surface of the pressure plate 20 so that the first clearance hole 21 and the second clearance hole 31 are connected.

[0127] By moving the movable pressure block 30 and making the first clearance hole 21 and the second clearance hole 31 correspondingly connected, on the one hand, the movable pressure block 30 can avoid pressing on the marking area 203, and on the other hand, the marking area can be observed through the first clearance hole 21 and the second clearance hole 31.

[0128] In some embodiments, step S400, adding a reaction reagent to the microfluidic chip assembly 200 to carry out the reaction, includes:

[0129] S410. Add markers to the microfluidic chip assembly 200 through the reagent window 22 of the pressure plate 20;

[0130] S420. The microfluidic chip assembly 200 is attracted by negative pressure through the negative pressure window 23 of the pressure plate 20.

[0131] Optionally, the process of adding markers can be completed by the experimenter operating a pipette or by a robotic arm; there is no limitation here.

[0132] In some embodiments, step S420 above, applying negative pressure to the microfluidic chip assembly 200 through the negative pressure window 23 of the pressure plate 20, includes:

[0133] S421. The cantilever 313 drives the negative pressure suction cup 311 to move to the negative pressure window 23, and the negative pressure suction cup 311 is sealed to the negative pressure window 23.

[0134] S422. Negative pressure suction cup 311 performs negative pressure suction on negative pressure window 23;

[0135] S423. The cantilever 313 drives the negative pressure suction cup 311 away from the negative pressure window 23.

[0136] When the negative pressure suction cup 311 is used, the cantilever 313 can rotate to connect with the multi-omics experimental device 100. When the negative pressure suction cup 311 is not used, the cantilever 313 can rotate the negative pressure suction cup 311 away from the multi-omics experimental device 100 by a certain distance, avoiding interference with other experimental operations of the multi-omics experimental device 100. The cantilever 313 helps to improve the convenience and automation of experimental operations.

[0137] In some embodiments, before performing step S600, removing the movable pressure block 30 from the surface of the pressure plate 20, the spatial multi-omics experimental method further includes:

[0138] S601. Add cleaning solution to the microfluidic chip assembly 200 through the reagent window 22 of the pressure plate 20 to flush the flow channels of the flow channel chip 201.

[0139] By setting a cleaning step to flush the channels of the flow channel chip 201, the markers in the channels can be removed before the glass plate 202 is separated. This prevents the markers from contaminating each channel after the glass plate 202 is separated from the flow channel chip 201, ensuring that the markers in each channel do not mix, thereby improving the accuracy and reliability of the marking.

[0140] In some embodiments, after implementing step S601, adding cleaning solution to the microfluidic chip assembly 200 through the reagent window 22 of the pressure plate 20, the spatial multi-omics experimental method further includes:

[0141] S6011. Remove the glass plate 202 from the microfluidic chip assembly 200;

[0142] S6012. Remove the cleaning solution from the surface of glass slide 202;

[0143] S6013. Drying glass slide 202.

[0144] After cleaning the flow channel chip 201 and separating the glass slide 202, the glass slide 202 can be cleaned again. This removes any residual markers on the glass slide 202, preventing them from affecting subsequent observation. Drying the glass slide 202 by blowing it dry prevents residual cleaning solution from affecting subsequent second marking or observation operations.

[0145] In some embodiments, step S700, removing the microfluidic chip assembly 200, includes:

[0146] S710. Fix the flow channel chip 201 of the microfluidic chip assembly 200 in the mounting base 10;

[0147] S720. The first lifting member 51 is pushed out in the vertical direction to lift the edge of the glass plate 202 of the microfluidic chip assembly 200 to separate it from the flow channel chip 201 of the microfluidic chip assembly 200.

[0148] By applying an upward force to the glass plate 202 using the first lifting component 51, the glass plate 202 can be separated from the flow channel chip 201, improving the ease of operation and automation, and avoiding manual operation by experimental personnel.

[0149] In some embodiments, step S710, fixing the flow channel chip 201 of the microfluidic chip assembly 200 in the mounting base 10, includes:

[0150] S711. The second lifting member 52 extends horizontally and abuts against the side of the flow channel chip 201 to fix the flow channel chip 201 in the mounting base 10.

[0151] By using the second lifting member 52 to fix the flow channel chip 201, it is possible to prevent the flow channel chip 201 from moving along with the glass sheet 202 when the glass sheet 202 is lifted, thus ensuring that the glass sheet 202 and the flow channel chip 201 can be separated smoothly.

[0152] In some embodiments, step S200, covering the surface of the microfluidic chip assembly 200 with the pressure plate 20, includes:

[0153] S210. When the pressure plate 20 covers the surface of the microfluidic chip assembly 200, the magnetic switch of the pressure plate 20 is connected to the magnetic connector of the mounting base 10, and the heating structure 24 in the pressure plate 20 is energized and heated.

[0154] Heating the pressure plate 20 can increase the temperature of the microfluidic chip assembly 200, which helps to improve the labeling effect and experimental efficiency of the marker under appropriate temperature conditions.

[0155] In some embodiments, while performing step S400 above—adding reactants to the microfluidic chip assembly 200 to carry out the reaction—the space multi-omics experimental method further includes:

[0156] S430. Use cover 322 to close box 321;

[0157] S440. Start the heating module 330 to heat the environment inside the insulation box 320.

[0158] By utilizing the combined action of the heating module 330 and the incubator 320, the incubator 320 can be maintained at a suitable temperature under the heating effect of the heating module 330, thereby providing a suitable environment for the binding of the cells and tissues to be labeled with the label, which helps to improve experimental efficiency and experimental results.

[0159] In some embodiments, step S400, adding a reaction reagent to the microfluidic chip assembly 200 to carry out the reaction, includes:

[0160] S450. Move the microscope to the second clearance hole 31 and observe the marking area 203 of the microfluidic chip assembly 200 through the second clearance hole 31 and the first clearance hole 21.

[0161] Microscopic observation allows for timely understanding of the degree of binding between the marker and cell tissues, which helps in the rational control of the reaction progress.

[0162] In some embodiments, prior to step S450, moving the microscope to the second clearance hole 31, the spatial multi-omics experimental method further includes:

[0163] S451. Fold the cover 322 to open the box 321 so that the multi-omics experimental apparatus is exposed in the opening 3211 of the box 321.

[0164] After the opening 3211 of the housing 321 is opened, the microscope can enter the space of the housing 321 to observe the microfluidic chip assembly 200 in the multi-omics experimental device 100.

[0165] In some embodiments, please refer to Figure 13 The above step S400, adding reaction reagents to the microfluidic chip assembly 200 to carry out the reaction, includes:

[0166] S461. Add the first round of markers to the microfluidic chip assembly 200;

[0167] S462. Add cleaning fluid to the microfluidic chip assembly 200 to flush the channels of the channel chip 201;

[0168] S463. Remove the glass plate 202 from the microfluidic chip assembly 200;

[0169] S464. Cover and connect the glass plate 202 with another flow channel chip 201 to form another microfluidic chip assembly 200;

[0170] S465. Add a second marker to the microfluidic chip assembly 200, wherein the marking direction of the second marker is set at an angle to the marking direction of the first marker.

[0171] By adding markers in two rounds, the cells on the glass slide 202 can be labeled in two different directions. Therefore, the cells can carry two dimensions of label information. Using these two dimensions of label information, the label position can be uniquely determined, thereby achieving the encoding of the spatial coordinates of the cell molecules.

[0172] Optionally, the marking directions of the first and second markers are perpendicular to each other. The method for adding the second marker is the same as that for adding the first marker, and will not be repeated here.

[0173] The embodiments of this application have been described in detail above with reference to the accompanying drawings. However, this application is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this application. Furthermore, unless otherwise specified, the embodiments and features described in the embodiments of this application can be combined with each other.

Claims

1. A multi-omics experiment device, characterized by: The application relates to a multi-omics experiment device. The mounting seat is used for placing a microfluidic chip assembly; The pressing plate is used for covering the surface of the microfluidic chip assembly, and the pressing plate is provided with a clearance structure corresponding to the marking area of the microfluidic chip assembly; The movable pressing block movably abuts against the upper surface of the pressing plate and is used for applying a pressing force to the pressing plate.

2. The multi-omics experiment device of claim 1, wherein: The clearance structure comprises a first clearance hole penetrating through the two side surfaces of the pressing plate, and at least part of the marking area of the microfluidic chip assembly is exposed to the first clearance hole.

3. The multi-omics experiment device of claim 2, wherein: The marking area of the microfluidic chip assembly is located in the projection area of the first clearance hole in the microfluidic chip assembly.

4. The multi-omics experiment device of claim 2, wherein: The movable pressing block is provided with a second clearance hole penetrating through the two side surfaces of the movable pressing block, and when the movable pressing block moves to the surface of the pressing plate, the second clearance hole is in communication with the first clearance hole.

5. The multi-omics experiment device of claim 4, wherein: The projection of the first clearance hole and the second clearance hole on the pressing plate is coincident.

6. The multi-omics experiment device according to any one of claims 2 to 5, wherein: The microfluidic chip assembly comprises a liquid inlet area, a connecting area and a liquid outlet area connected in sequence, and the marking area of the microfluidic chip assembly is located in the connecting area; The movable pressing block is in a strip shape, and the movable pressing block is arranged at least corresponding to the connecting area, and the first clearance hole is arranged corresponding to the marking area.

7. The multi-omics experiment device of claim 6, wherein: The pressing plate is further provided with a reagent window, the reagent window is arranged corresponding to the liquid inlet area of the microfluidic chip assembly, and the reagent window is arranged in a spaced manner with the first clearance hole; The multi-omics experiment device comprises at least two movable pressing blocks, one of the movable pressing blocks is arranged corresponding to the connecting area, and the remaining movable pressing blocks are arranged at least corresponding to the outer periphery of the reagent window of the microfluidic chip assembly.

8. The multi-omics experiment device of claim 6, wherein: The pressing plate is further provided with a negative pressure window, the negative pressure window is arranged corresponding to the liquid outlet area of the microfluidic chip assembly, and the negative pressure window is arranged in a spaced manner with the first clearance hole; The multi-omics experiment device comprises at least two movable pressing blocks, one of the movable pressing blocks is arranged corresponding to the connecting area, and the remaining movable pressing blocks are arranged at least corresponding to the outer periphery of the negative pressure window of the microfluidic chip assembly.

9. The multi-omics experiment device of claim 1, wherein: When the movable pressing block moves to the surface of the pressing plate, the movable pressing block avoids the clearance structure.

10. The multi-omics experiment device of claim 9, wherein: The clearance structure comprises a first clearance hole penetrating through the two side surfaces of the pressing plate, and the minimum distance from the edge of the first clearance hole to the edge of the pressing plate is D, and when the movable pressing block moves to the surface of the pressing plate, the length of the movable pressing block on the pressing plate is less than or equal to D.

11. The multi-omics experiment device of claim 10, wherein: The multi-omics experiment device comprises two movable pressing blocks, and when the movable pressing blocks move to the surface of the pressing plate, the two movable pressing blocks are arranged in a symmetrical manner about the first clearance hole.

12. The multi-omics experiment device of any one of claims 1 to 5, wherein: The multi-omics experiment device further comprises a base, the mounting seat is arranged on the base, and the movable pressing block is rotationally connected to the base, so that the movable pressing block is rotated to the surface of the pressing plate or is rotated away from the surface of the pressing plate.

13. The multi-omics experiment device of claim 1, wherein: The movable pressing block is a pneumatic pressing block.

14. The multi-omics experiment device of claim 1, wherein: The movable pressing block is an electric pressing block.

15. The multi-omics experiment device of claim 1, wherein: The multi-omics experimental device further comprises a base, the mounting seat is detachably connected to the base, the mounting seat is provided with a first limiting part, the base is provided with a second limiting part, when the mounting seat is arranged on the base, the first limiting part and the second limiting part are connected in cooperation.

16. The multi-omics experiment device of claim 15, wherein: The first limiting part is a limiting hole, the second limiting part is a protrusion column protruding from the surface of the mounting seat, and the protrusion column can be inserted into the limiting hole.

17. The multi-omics experiment device of claim 16, wherein: The mounting seat is further provided with a third limiting part, the third limiting part protrudes from the top surface of the mounting seat, the protruding height of the third limiting part is greater than that of the second limiting part, and the third limiting part is used for limiting the pressing plate.

18. The multi-omic experiment device of claim 1, wherein: The pressing plate is provided with a heating structure.

19. The multi-omics experiment device of claim 18, wherein: The pressing plate is provided with a first magnetic attraction joint, the first magnetic attraction joint is arranged towards the mounting seat, the mounting seat is provided with a second magnetic attraction joint, when the pressing plate is arranged on the mounting seat, the first magnetic attraction joint is connected with the second magnetic attraction joint to conduct the heating structure.

20. The multi-omics experiment device of claim 18 or 19, wherein: The pressing plate is further provided with a temperature sensor.

21. The multi-omic experiment device of claim 1, wherein: The surface of the pressing plate for covering the microfluidic chip assembly is further provided with a sealing gasket, and the sealing gasket is sealed between the pressing plate and the microfluidic chip assembly.

22. The multi-omic experiment device of claim 1, wherein: The pressing plate is provided with a first positioning structure, the mounting seat is provided with a second positioning structure, when the pressing plate covers the microfluidic chip assembly, the first positioning structure and the second positioning structure are connected in position.

23. The multi-omics experiment device of claim 22, wherein: The first positioning structure comprises a positioning notch, the second positioning structure comprises a positioning protrusion column, and when the pressing plate covers the microfluidic chip assembly, the positioning protrusion column extends into the positioning notch.

24. The multi-omic experiment device of claim 1, wherein: The surface of the pressing plate towards the microfluidic chip assembly is provided with a negative pressure groove, the bottom of the negative pressure groove is provided with a negative pressure window, the negative pressure window penetrates the surface of the pressing plate, the negative pressure groove is arranged corresponding to the liquid outlet area of the microfluidic chip assembly, and the negative pressure window is arranged in interval with the first avoiding hole arranged on the pressing plate.

25. The multi-omic experiment device of claim 1, wherein: The multi-omics experimental device further comprises an ejection assembly, the ejection assembly comprises a first lifting piece that can be lifted in a vertical direction, the first lifting piece is arranged outside the mounting seat, and the first lifting piece is used for lifting the glass sheet of the microfluidic chip assembly to separate the flow channel chip of the microfluidic chip assembly.

26. The multi-omics experiment device of claim 25, wherein: The ejection assembly further comprises a second lifting piece that can be ejected in a horizontal direction, the second lifting piece is arranged outside the mounting seat, and the second lifting piece is arranged on the same side or different side of the first lifting piece, the second lifting piece is used for abutting the side surface of the flow channel chip of the microfluidic chip assembly when being ejected, so as to fix the flow channel chip in the mounting seat.

27. The multi-omic experiment device of claim 25, wherein: The ejection assembly further comprises a suction accessory, the suction accessory is arranged at least on the bottom surface or the side surface of the mounting seat, and the suction accessory is used for adsorbing and fixing the flow channel chip of the microfluidic chip assembly.

28. The multi-omics experiment apparatus of any one of claims 25 to 27, wherein: The multi-omics experimental device further comprises a microfluidic chip assembly, the microfluidic chip assembly comprises a glass sheet and a flow channel chip, at least one side edge of the glass sheet is further provided with a protruding part, when the glass sheet covers the upper surface of the flow channel chip, the protruding part protrudes from the side surface of the flow channel chip. The first jacking member is abutted against the protruding part when being lifted to drive the glass sheet to separate from the flow channel chip.

29. The multi-omic experiment device of claim 1, wherein: The surface of the mounting seat is further provided with a positioning groove for placing the microfluidic chip assembly.

30. The multi-omic experiment device of claim 1, wherein: The multi-omics experimental device further comprises a base, the mounting seat is arranged on the base, and the base is provided with a waste liquid collecting groove and a flow outlet communicated with the waste liquid collecting groove.

31. An experimental apparatus, characterized by: The multi-omics experimental device comprises a negative pressure module, and the negative pressure module is movably connected to a negative pressure window of the multi-omics experimental device, and the negative pressure module is used for negative pressure suction of a flow channel in the microfluidic chip assembly.

32. The experimental setup of claim 31, wherein: The negative pressure module comprises a negative pressure suction disc, and the negative pressure suction disc is movably connected to the negative pressure window of the multi-omics experimental device, and the negative pressure suction disc is sealingly connected to the negative pressure window.

33. The experimental apparatus of claim 32, wherein: The negative pressure module comprises a base and a cantilever which are rotationally connected to each other, the negative pressure suction disc is arranged on the cantilever, and the cantilever is used to drive the negative pressure suction disc to be connected to the negative pressure window or to be removed from the negative pressure window.

34. The experimental apparatus of claim 31, wherein: The experimental equipment further comprises an incubator and a heating module, the multi-omics experimental device, the negative pressure module and the heating module are arranged in the incubator, and the heating module is used to provide heat to the incubator.

35. The experimental apparatus of claim 34, wherein: The heating module comprises a heating element and a heat dissipation structure, and the heat dissipation structure is connected to the heating element.

36. The experimental apparatus of claim 35, wherein: The negative pressure module comprises a negative pressure suction disc and a base and a cantilever which are rotationally connected to each other, the negative pressure suction disc is arranged on the cantilever, the heating module is arranged on the base, and the heating module avoids the cantilever.

37. The experimental apparatus of claim 34, wherein: The incubator comprises a box body and a cover body, the box body is used to accommodate the multi-omics experimental device, the negative pressure module and the heating module, the box body is provided with an opening, and the cover body can cover or open the opening.

38. The experimental setup of claim 37, wherein: The opening is arranged at the top of the box body, and the cover body can be foldably arranged on one side of the opening to open the opening, or the cover body can be unfolded to cover the opening.

39. The experimental apparatus of claim 38, wherein: The incubator further comprises a linear module, the linear module is arranged at the opening, the cover body is connected to the linear module, and the linear module is used to drive the cover body to be folded or unfolded along the extension direction of the linear module.

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