A laminated centrifugal filtration device for isolating and extracting exosomes

Abstract

This utility model discloses a stacked centrifugal filtration device for separating and extracting exosomes, including a cylindrical body. Multiple fixed rings are fixed inside the cylindrical body, and a rotating ring is rotatably connected inside each fixed ring. A filter screen is fixed inside the rotating ring, and a fixed plate is fixed to the bottom of the filter screen. Multiple support rods are fixed to the top of the fixed plate. The raw liquid enters the filter screen through a feed pipe. A motor is started, causing a circular rod to rotate. This circular rod rotates a fixed frame, a connecting rod, and the rotating ring. The rotating ring rotates the filter screen and the fixed plate. The fixed plate, through a speed-increasing mechanism, rotates the lower circular rod and the filter screen. This allows for centrifugal filtration of the raw liquid. The filtered liquid enters a funnel and then the next filter screen for further filtration. The filtration gaps in the filter screen decrease sequentially from top to bottom. This process is repeated multiple times to filter the raw liquid, improving filtration efficiency and purity.

Description

Technical Field

[0001] This utility model relates to the technical field of extraction and filtration devices, specifically a stacked centrifugal filtration device for separating and extracting exosomes. Background Technology

[0002] Exosomes are small membrane vesicles (30-150 nm) containing complex RNA and proteins; currently, they specifically refer to disc-shaped vesicles with a diameter of 40-100 nm. Exosomes were first discovered in sheep reticulocytes in 1983, and Johnstone named them "exosome" in 1987. Various cell types can secrete exosomes under both normal and pathological conditions. They mainly originate from multivesicles formed by the invagination of lysosomal microparticles within the cell, and are released into the extracellular matrix after the outer membrane of the multivesicle fuses with the cell membrane.

[0003] Currently, the main methods for extracting exosomes can be summarized as follows: ultracentrifugation, polymer precipitation, magnetic bead method, and ultrafiltration. Ultracentrifugation separates exosomes by rotating a filter screen. Existing centrifugal filtration devices use a single filter screen, which results in low purity and low filtration efficiency. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a stacked centrifugal filtration device for separating and extracting exosomes, which solves the problems mentioned in the background section.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a stacked centrifugal filtration device for separating and extracting exosomes, comprising a cylindrical body, wherein multiple fixed rings are fixed inside the cylindrical body, a rotating ring is rotatably connected inside the fixed rings, a filter mesh ring is fixed inside the rotating ring, a fixed plate is fixed at the bottom of the filter mesh ring, multiple support rods are fixed at the top of the fixed plate, and the two ends of the support rods are respectively fixedly connected to the fixed plate of the rotating ring, a fixed frame is provided inside the filter mesh ring, and multiple connecting rods are fixed at the top of the rotating ring;

[0006] The other end of the connecting rod is fixedly connected to the fixing frame. A round rod is fixed to the top of the fixing frame. A motor is fixed to the top of the cylinder. The output shaft of the motor is fixedly connected to the top of the uppermost round rod. An acceleration mechanism is provided below the two upper fixing plates. Multiple fixing rods are fixed to the outer wall of the acceleration mechanism. The other end of the fixing rod is fixedly connected to the inner wall of the cylinder. A feed pipe is fixed to the top of the cylinder.

[0007] Preferably, the bottom of the cylinder is fixed with multiple support legs.

[0008] Preferably, a funnel is fixed inside the cylinder above the two lower filter rings.

[0009] Preferably, the speed-increasing mechanism includes a housing, an input shaft rotatably connected to the upper part of the housing, a connecting plate fixed to the bottom end of the input shaft, a gear ring fixed to the bottom of the connecting plate, a plurality of driven gears inside the gear ring, the bottom of the driven gears rotatably connected to the lower inner surface of the housing, an output shaft rotatably connected to the lower inner surface of the housing, a master gear fixed to the top of the output shaft, the driven gears meshing with the gear ring, the input shaft fixedly connected to the bottom of the fixing plate, and the output shaft fixedly connected to the lower round rod.

[0010] Preferably, the fixed plate has a sealing block inside, the top of the sealing block is fixed with a ring, the top of the ring is rotatably connected with a connecting ring, the top of the connecting ring is fixed with a moving rod, two moving rings are provided above the uppermost moving rod, the moving rings are rotatably connected to each other, the top of the uppermost moving rod is fixedly connected to the bottom of the lower moving ring, the tops of the two lower moving rods are fixedly connected to the bottom of the sealing block, a water inlet pipe is fixedly fixed to the top of the cylinder, a support plate is fixedly fixed to the top of the cylinder, a hydraulic rod is fixedly fixed to the bottom of the support plate, and the output shaft of the hydraulic rod is fixedly connected to the upper moving ring.

[0011] Preferably, the inner side of the ring is slidably connected with a plurality of limiting rods, and the bottom of the limiting rods is fixedly connected to the top of the fixing plate. Beneficial effects

[0012] This invention provides a stacked centrifugal filtration device for separating and extracting exosomes. Compared with the prior art, it has the following advantages:

[0013] 1. This stacked centrifugal filtration device for separating and extracting exosomes comprises a motor, a round rod, a fixed frame, a connecting rod, a speed-increasing mechanism, a support rod, and a fixed plate. The raw liquid enters the filter ring through the feed pipe. When the motor is started, it drives the round rod to rotate, which in turn drives the fixed frame, connecting rod, and rotating ring. The rotating ring drives the filter ring and fixed plate to rotate, and the fixed plate, through the speed-increasing mechanism, drives the lower round rod and filter ring to rotate. This process centrifugally filters the raw liquid. The filtered liquid enters a funnel and then the next filter ring for further filtration. The filtration gaps in the filter ring decrease progressively from top to bottom. This process is repeated multiple times to filter the raw liquid, improving filtration efficiency and purity.

[0014] 2. This stacked centrifugal filtration device for separating and extracting exosomes consists of a blocking block, a circular ring, a connecting ring, a moving ring, a moving rod, and a hydraulic rod. When the hydraulic rod is activated, it moves the moving ring, which in turn moves the upper moving rod. The upper moving rod then moves the connecting ring, the circular ring, and the blocking block. The blocking block separates from the fixed plate, and cleaning water enters through the inlet pipe to clean the filter screen. The wastewater from the cleaning process drains down from the fixed plate, thus removing impurities from the filter screen. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a cross-sectional view of the middle cylinder of this utility model;

[0017] Figure 3 This is a cross-sectional view of the filter mesh ring in this utility model;

[0018] Figure 4 This is a cross-sectional view of the shell structure in this utility model.

[0019] In the diagram: 1. Support plate; 2. Hydraulic rod; 3. Support leg; 4. Cylinder; 5. Feed pipe; 6. Motor; 7. Water inlet pipe; 8. Fixing plate; 9. Filter screen; 10. Support rod; 11. Connecting rod; 12. Funnel; 13. Fixing rod; 14. Rotary ring; 15. Round rod; 16. Speed-increasing mechanism; 1601. Housing; 1602. Input shaft; 1603. Driven gear; 1604. Output shaft; 1605. Main gear; 1606. Connecting plate; 1607. Gear ring; 17. Fixing ring; 18. Moving rod; 19. Fixing frame; 20. Connecting ring; 21. Moving ring; 22. Sealing block; 23. Circular ring; 24. Limiting rod. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figures 1-4This utility model provides a technical solution: a stacked centrifugal filtration device for separating and extracting exosomes, comprising a cylindrical body 4, with multiple fixed rings 17 fixed inside the cylindrical body 4, a rotating ring 14 rotatably connected inside the fixed rings 17, a filter mesh ring 9 fixed inside the rotating ring 14, a fixed plate 8 fixed at the bottom of the filter mesh ring 9, and multiple support rods 10 fixed at the top of the fixed plate 8. The two ends of the support rods 10 are respectively fixedly connected to the fixed plate 8 of the rotating ring 14. A fixed frame 19 is provided inside the filter mesh ring 9, and multiple connecting rods 11 are fixed at the top of the rotating ring 14. The other end of the connecting rods 11 is fixedly connected to the fixed frame 19. A round rod 15 is fixed to the top of the fixed frame 19, and a motor 6 is fixed to the top of the cylinder 4. The output shaft of the motor 6 is fixedly connected to the top of the uppermost round rod 15. An acceleration mechanism 16 is provided below the two upper fixed plates 8. Multiple fixed rods 13 are fixed to the outer wall of the acceleration mechanism 16, and the other end of the fixed rods 13 is fixedly connected to the inner wall of the cylinder 4. A feed pipe 5 is fixed to the top of the cylinder 4. This process is repeated multiple times to filter the raw liquid, increasing the filtration efficiency and purity. Multiple support legs 3 are fixed to the bottom of the cylinder 4 to raise the height of the device. The interior of the cylinder 4 is located in the lower two... A funnel 12 is fixed above each filter ring 9 to facilitate the filtered liquid entering the interior of the lower filter ring 9. The speed-increasing mechanism 16 includes a housing 1601. An input shaft 1602 is rotatably connected to the upper part of the housing 1601. A connecting plate 1606 is fixed to the bottom end of the input shaft 1602. A gear ring 1607 is fixed to the bottom of the connecting plate 1606. Multiple driven gears 1603 are provided inside the gear ring 1607. The bottom of the driven gears 1603 is rotatably connected to the lower surface of the housing 1601. An output shaft 1604 is rotatably connected to the lower part of the housing 1601. The top of the output shaft 1604... A main gear 1605 is fixed, and a driven gear 1603 and a gear ring 1607 are meshed together. The input shaft 1602 is fixedly connected to the bottom of the fixed plate 8, and the output shaft 1604 is fixedly connected to the lower round rod 15. The fixed plate 8 rotates the input shaft 1602, which in turn rotates the connecting plate 1606 and the gear ring 1607. One rotation of the gear ring 1607 causes the driven gear 1603 to rotate multiple times. The driven gear 1603 drives the main gear 1605 and the output shaft 1604 to rotate synchronously. This increases the rotational speed of the output shaft 1604, thereby increasing the rotational speed of the lower filter ring 9 and improving the filtration efficiency.

[0022] Furthermore, the fixed plate 8 has a sealing block 22 inside, and a ring 23 is fixed to the top of the sealing block 22. A connecting ring 20 is rotatably connected to the top of the ring 23. A moving rod 18 is fixed to the top of the connecting ring 20. Two moving rings 21 are provided above the uppermost moving rod 18 and are rotatably connected to each other. The top of the uppermost moving rod 18 is fixedly connected to the bottom of the lower moving ring 21, and the tops of the two lower moving rods 18 are fixedly connected to the bottom of the sealing block 22. A water inlet pipe 7 is fixed to the top of the cylinder 4, and a support plate 1 is fixed to the top of the cylinder 4. A hydraulic rod 2 is fixed to the bottom of the support plate 1. The output shaft of the hydraulic rod 2 is fixedly connected to the upper moving ring 21. This allows for cleaning of the filter ring 9. Multiple limiting rods 24 are slidably connected inside the ring 23. The bottom of the limiting rods 24 is fixedly connected to the top of the fixed plate 8, which restricts the rotation of the ring 23.

[0023] During operation, the raw liquid enters the upper filter ring 9 through the feed pipe 5. The motor 6 is started, causing the round rod 15 to rotate. The round rod 15, in turn, rotates the fixed frame 19, connecting rod 11, and rotating ring 14. The rotating ring 14 then rotates the filter ring 9 and the fixed plate 8. The fixed plate 8, via the speed-increasing mechanism 16, rotates the lower round rod 15 and the filter ring 9. This centrifugal filtration of the raw liquid is achieved. The filtered liquid enters the funnel 12 and then the next filter ring 9 for further filtration. The filter ring 9 has increasingly larger filtration gaps from top to bottom. The filter is reduced in size and then filtered multiple times to increase filtration efficiency and purity. After filtration is complete, hydraulic rod 2 is activated, which moves moving ring 21. Moving ring 21 moves moving upper moving rod 18, which in turn moves connecting ring 20, circular ring 23, and sealing block 22. Sealing block 22 separates from fixed plate 8, and cleaning water enters from inlet pipe 7 to clean the filter screen 9. The wastewater after cleaning drains from fixed plate 8, thus cleaning impurities on the filter screen 9.

[0024] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0025] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, the phrase "comprising an element defined as..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0026] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A stacked centrifugal filtration device for separating and extracting exosomes, comprising a cylindrical body (4), characterized in that: The cylinder (4) has multiple fixed rings (17) inside, and a rotating ring (14) is rotatably connected inside the fixed rings (17). A filter mesh ring (9) is fixed inside the rotating ring (14). A fixed plate (8) is fixed at the bottom of the filter mesh ring (9). Multiple support rods (10) are fixed at the top of the fixed plate (8). The two ends of the support rods (10) are fixedly connected to the fixed plate (8) of the rotating ring (14). A fixed frame (19) is provided inside the filter mesh ring (9). Multiple connecting rods (11) are fixed at the top of the rotating ring (14). The other end of the connecting rod (11) is fixedly connected to the fixing frame (19). A round rod (15) is fixed to the top of the fixing frame (19). A motor (6) is fixed to the top of the cylinder (4). The output shaft of the motor (6) is fixedly connected to the top of the uppermost round rod (15). An acceleration mechanism (16) is provided below the two upper fixing plates (8). Multiple fixing rods (13) are fixed to the outer wall of the acceleration mechanism (16). The other end of the fixing rod (13) is fixedly connected to the inner wall of the cylinder (4). A feed pipe (5) is fixed to the top of the cylinder (4).

2. The stacked centrifugal filtration device for separating and extracting exosomes according to claim 1, characterized in that: The bottom of the cylinder (4) is fixed with multiple support legs (3).

3. The stacked centrifugal filtration device for separating and extracting exosomes according to claim 2, characterized in that: Inside the cylinder (4), above the two filter rings (9) below, there are funnels (12) fixed.

4. The stacked centrifugal filtration device for separating and extracting exosomes according to claim 3, characterized in that: The speed-increasing mechanism (16) includes a housing (1601), an input shaft (1602) is rotatably connected to the upper part of the housing (1601), a connecting plate (1606) is fixed to the bottom end of the input shaft (1602), a gear ring (1607) is fixed to the bottom of the connecting plate (1606), a plurality of driven gears (1603) are provided inside the gear ring (1607), the bottom of the driven gears (1603) is rotatably connected to the lower inner surface of the housing (1601), an output shaft (1604) is rotatably connected to the lower inner part of the housing (1601), a master gear (1605) is fixed to the top of the output shaft (1604), the driven gears (1603) and the gear ring (1607) are meshed, the input shaft (1602) is fixedly connected to the bottom of the fixing plate (8), and the output shaft (1604) is fixedly connected to the lower round rod (15).

5. The stacked centrifugal filtration device for separating and extracting exosomes according to claim 4, characterized in that: The fixed plate (8) is provided with a sealing block (22) inside. A ring (23) is fixed on the top of the sealing block (22). A connecting ring (20) is rotatably connected to the top of the ring (23). A moving rod (18) is fixed on the top of the connecting ring (20). Two moving rings (21) are provided above the uppermost moving rod (18). The moving rings (21) are rotatably connected to each other. The top of the uppermost moving rod (18) and the bottom of the lower moving ring (21) are fixedly connected. The tops of the two lower moving rods (18) and the bottoms of the sealing block (22) are fixedly connected. A water inlet pipe (7) is fixed on the top of the cylinder (4). A support plate (1) is fixed on the top of the cylinder (4). A hydraulic rod (2) is fixed on the bottom of the support plate (1). The output shaft of the hydraulic rod (2) is fixedly connected to the upper moving ring (21).

6. The stacked centrifugal filtration device for separating and extracting exosomes according to claim 5, characterized in that: The inner side of the ring (23) is slidably connected with a plurality of limiting rods (24), the bottom of the limiting rods (24) and the top of the fixing plate (8) are fixedly connected.

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