A disposable cell filtration collector

Abstract

This utility model discloses a disposable cell filter collector, comprising: a column having a through cavity; a column cap, which is sealed to the column to form a filter cavity; a filter membrane located within the filter cavity and disposed on a sieve plate; a sieve plate located within the filter cavity for supporting the filter membrane; and a rubber stopper located at the lower end of the column cap, having a through vent hole for sealing the connection between the collector and external instruments. Based on cell filtration using a filter membrane, this utility model, by setting a sieve plate structure adapted to the pore size of the filter membrane, effectively supports the filter membrane during the filtration process, preventing deformation or even damage due to the filter membrane's inability to withstand fluid impact; simultaneously, the sieve plate and filter membrane work together to complete cell filtration and enrichment.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a disposable cell filter collector. Background Technology

[0002] Circulating tumor cells (CTCs) are present in very small numbers in peripheral blood, typically requiring the isolation of a small number of tumor cells from a large pool of blood cells. Current cell filters often employ complex hollow membrane holders and filter screens, requiring connection via ultrasonic welding or thermal fusion, demanding precise handling. Furthermore, solid membrane holders can negatively impact filtration efficiency. For example, CN210458181U discloses a cell filter, but its complex structure and high production cost make it unsuitable for use by non-professionals. Utility Model Content

[0003] To solve the above-mentioned technical problems, this utility model provides a disposable cell filter collector.

[0004] Specifically, this utility model discloses a disposable cell filter collector, comprising:

[0005] A column with a through cavity, and a connecting part at its lower end that connects to a column cap;

[0006] The column cap is sealed to the column body to form a filter cavity;

[0007] A filter membrane is located within the filter cavity and disposed on a sieve plate;

[0008] A sieve plate, located inside the filter cavity, is used to support the filter membrane. The end face of the sieve plate that contacts the filter membrane has several through holes.

[0009] A rubber stopper, located at the lower port of the column cap, has a vent hole for sealing the connection between the collector and external instruments.

[0010] Furthermore, the filter membrane is an inverted disc-shaped structure, and the sieve plate is a circular pad embedded in the recess of the disc-shaped structure.

[0011] Furthermore, it also includes a sealing ring disposed between the column body and the column cap.

[0012] Furthermore, the end face of the rubber stopper located at one end of the cap has a concave surface structure.

[0013] Furthermore, a flange is provided on the upper outer edge of the connecting part, the sealing ring is fitted on the connecting part and located below the flange, the column cap is fitted on the connecting part, and the sealing connection is achieved by pressing the sealing ring through the stepped structure on the inner wall of the column cap.

[0014] Furthermore, the upper end of the column cap and the flange are provided with a matching one-way self-locking clamping structure.

[0015] Furthermore, the vent hole of the rubber plug is provided with a diaphragm for sealing.

[0016] The disposable cell filter collector of this invention, based on a filter membrane for cell filtration, incorporates a sieve plate structure adapted to the pore size of the filter membrane. This sieve plate effectively supports the filter membrane during filtration, preventing deformation or damage due to the membrane's inability to withstand fluid impact. Simultaneously, the sieve plate and filter membrane work together to filter and enrich cells. This disposable cell filter collector of this invention has a simple structure, is easy to install, and has strong industrial applicability. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0018] Figure 1 This is a schematic diagram of the structure of a disposable cell filter collector according to the present invention;

[0019] Figure 2 This is a schematic diagram of the overall assembly structure;

[0020] Figure 3 This is a schematic diagram of a one-way self-locking mechanism between the column and the column cap;

[0021] Figure 4 This is a schematic diagram of the filter membrane structure;

[0022] Figure 5 This is a schematic diagram of the sieve plate structure;

[0023] Figure 6 This is a schematic diagram of the assembly of the filter membrane and the sieve plate;

[0024] Figure 7 Schematic diagram of rubber stopper structure

[0025] Figure 8 This is a schematic diagram illustrating the installation of the disposable cell filter collector of this utility model during use.

[0026] Figure 9 This is a structural diagram showing the installation of the receiving seat and the puncture needle. Detailed Implementation

[0027] The present invention will now be described in further detail with reference to the accompanying drawings.

[0028] like Figures 1 to 7As shown, a disposable cell filter collector includes:

[0029] The column 1 has a through cavity, and its lower end is provided with a connecting part that connects to the column cap 2;

[0030] The column cap 2 is sealed to the column body 1 to form a filter cavity;

[0031] Filter membrane 4 is located inside the filter cavity and disposed on the sieve plate 5;

[0032] The sieve plate 5 is located inside the filter cavity and is used to support the filter membrane 4. The end face of the sieve plate 5 that contacts the filter membrane 4 has several through holes.

[0033] The rubber stopper 6, located at the lower port of the column cap 2, has a vent hole for sealing the connection between the collector and external instruments.

[0034] The column 1 is installed and connected to the column cap 2. The internal liquid path is connected by the receiving seat 7 and the puncture needle 9 on the cell sorter. The sieve plate 5 and the filter membrane 4 are connected to complete the filtration and enrichment of cells. Unwanted components are collected through the filter membrane, and the cells are enriched on the upper side of the filter membrane 4. The structure is simple and has strong applicability.

[0035] Furthermore, the filter membrane 4 is an inverted disc-shaped structure, and the sieve plate 5 is a circular pad embedded in the recess of the disc-shaped structure. Since the filter membrane 4 is inverted, during use, a liquid absorption action occurs first, which happens on the underside of the sieve plate 5. At this time, the sieve plate 5 acts as a support, protecting the filter membrane 4 from excessive deformation and effectively extending the service life of the entire device. Simultaneously, the sieve plate 5 is also provided with multiple through holes, allowing cells and liquids to pass through. Furthermore, the upper side of the sieve plate 4 is clamped and fixed to the protruding position inside the column cap 2 via a step located inside the column body 1, ensuring that it does not shake during use.

[0036] Furthermore, it also includes a sealing ring 3 disposed between the column body 1 and the column cap 2. The sealing ring effectively prevents leakage between the column body and the column cap 2, ensuring that the liquid inside the column body will not leak.

[0037] Furthermore, the end face of the rubber stopper 6 located at one end of the cap 2 has a concave structure, forming a cavity between it and the sieve plate 5. In use, the puncture needle 8 passes through the rubber stopper and extends into the cavity, located on the lower side of the sieve plate 5. At this time, the rubber stopper 6 can ensure that there is no leakage of liquid or air between the puncture needle and the rubber stopper 6, which facilitates subsequent liquid aspiration and filling. Liquid aspiration removes excess components, and the required cells are intercepted by the filter membrane 4. Then, liquid filling is performed, allowing the liquid to enter the upper side of the diaphragm 4. The cells intercepted on the filter membrane 4 and the liquid form a new cell suspension. The liquid in the cell filter collector is taken out with a pipette for subsequent pathological staining, molecular diagnostic and other experiments.

[0038] Furthermore, a flange is provided on the upper outer edge of the connecting part, the sealing ring 3 is sleeved on the connecting part and located below the flange, and the column cap 2 is sleeved on the connecting part. The sealing connection is achieved by pressing the sealing ring 3 through the stepped structure on the inner wall of the column cap 5. This also ensures that there is no leakage and guarantees its use.

[0039] Furthermore, the upper end of the column cap 2 and the flange are provided with a matching one-way self-locking clamping structure. The inner side of the column cap 2 is provided with a groove, and the flange has a protrusion. The two are installed by snap-fit ​​to form a clamping structure, ensuring that the column cap 2 and the column body 1 will not move.

[0040] The vent hole of the rubber stopper 6 is provided with a diaphragm 9 for sealing. The diaphragm plays a protective role, ensuring that the inside of the cap 2 is clean. At the same time, the diaphragm 9 is punctured by the puncturing needle 8, so that the puncturing needle 8 and the rubber stopper 6 are tightly attached, effectively preventing liquid and gas leakage.

[0041] This device is a consumable accessory for a cell sorter. The cell sorter is a device that physically sorts cells based on their size using a size-based filtration method. Since the cell sorter is existing equipment, it will not be described in detail here. The cell sorter is equipped with a receiving base 7, such as... Figure 8-9 As shown, it has an internal container for accommodating a disposable cell filter collector. A puncture needle 8 is located at the bottom of the container to form a liquid inlet, which is also connected to the internal liquid system.

[0042] The specific working principle is as follows: The sample is filtered by a filter membrane of a specific size in a disposable cell filter collector through the sorting control method of the cell sorter to remove impurities and enrich the target cells on the filter membrane; then, a high-purity target cell mixture is formed by reverse ejection of buffer solution.

[0043] Detailed operating steps: Follow Figure 1 After pressing and clamping all parts in the sequence shown, assembly is complete.

[0044] Insert one side of the cap 2 of the disposable cell filter collector into the liquid inlet of the matching instrument, i.e., into the receiver 7. The puncture needle 8 at the inlet pierces the diaphragm 9 inside the rubber stopper 6, thus connecting the cell filter collector to the liquid system of the matching instrument.

[0045] Add the processed sample to the disposable cell filter collector, click the sample processing function, the instrument first draws liquid downwards, and cells with small pore size in the liquid to be processed are removed by the liquid path through the filtration action of filter membrane 4 and sieve plate 5, while cells of the target size are retained on filter membrane 4.

[0046] The instrument ejects liquid upwards and backwards. The cells intercepted on the filter membrane 4 form a new cell suspension with the liquid. The liquid in the cell filter collector is then removed with a pipette for subsequent pathological staining, molecular diagnostic and other experiments.

[0047] The disposable cell filter collector of this invention, based on the cell filtration using a filter membrane 4, incorporates a sieve plate 5 structure adapted to the pore size of the filter membrane 4. On one hand, the sieve plate 5 effectively supports the filter membrane 4 during the filtration process, preventing deformation or damage due to the membrane's inability to withstand fluid impact. On the other hand, the sieve plate 5 and the filter membrane 4 work together to filter and enrich cells. This disposable cell filter collector of this invention has a simple structure, is easy to install, and has strong industrial applicability.

[0048] Existing technologies and market solutions for cell separation and enrichment often employ multi-layered filtration, such as using two consumables—a large-pore membrane and a small-pore membrane—or welding different materials together. This increases production and personnel costs and is inconvenient for non-professionals. In contrast, this application presents a simple, modular design, where assembly and sealing are achieved directly through the interlocking of the column and cap. It boasts high integration, requires no special processing, is easy to use and assemble, and is more suitable for practical applications.

[0049] It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the inventive concept of this utility model, and these all fall within the protection scope of this utility model.

Claims

1. A disposable cell filter collector, characterized in that, include: The column (1) has a through cavity and its lower end is provided with a connecting part that connects to the column cap (2); The column cap (2) is sealed to the column body (1) to form a filter cavity; A filter membrane (4) is located inside the filter cavity and disposed on a sieve plate (5); The sieve plate (5) is located inside the filter cavity and is used to support the filter membrane (4). The end face of the sieve plate (5) that contacts the filter membrane (4) has several through holes. A rubber stopper (6), located at the lower port of the cap (2), has a vent hole for sealing the connection between the collector and external instruments.

2. The disposable cell filter collector according to claim 1, characterized in that, The filter membrane (4) is an inverted disc-shaped structure, and the sieve plate (5) is a circular pad embedded in the recess of the disc-shaped structure.

3. The disposable cell filter collector according to claim 1, characterized in that, It also includes a sealing ring (3) disposed between the column (1) and the column cap (2).

4. The disposable cell filter collector according to claim 1, characterized in that, The rubber stopper (6) has a concave surface structure on one or both ends of the cap (2).

5. The disposable cell filter collector according to claim 3, characterized in that, A flange is provided on the upper outer edge of the connecting part. The sealing ring (3) is sleeved on the connecting part and located below the flange. The column cap (2) is sleeved on the connecting part, and the sealing connection is achieved by pressing the sealing ring (3) through the stepped structure on the inner wall of the column cap (2).

6. The disposable cell filter collector according to claim 5, characterized in that, The upper end of the column cap (2) is provided with a matching one-way self-locking clamping structure with the flange.

7. The disposable cell filter collector according to any one of claims 1-6, characterized in that, The vent of the rubber plug (6) is provided with a diaphragm (9) for sealing.

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