A chromatographic column backflush apparatus

Abstract

The utility model relates to the technical field of chromatographic column, concretely to a chromatographic column backwash device, including chromatographic column body, adjusting mechanism and storage tank, the input and output of chromatographic column body all are provided with adjusting seat, and the first connecting pipe, second connecting pipe and third connecting pipe are connected on adjusting seat, and adjusting mechanism is constituted by adjusting assembly setting on adjusting seat and two groups of blockades installed on adjusting assembly, and two groups of blockades are driven to rotate adjusting along adjusting seat inner chamber through adjusting assembly, the inside of storage tank is provided with cleaning agent chamber and waste liquid chamber, and the output of cleaning agent chamber is connected with three -way valve through the liquid pump, the utility model discloses setting adjusting mechanism on two adjusting seats respectively, and the convenient adjustment can be carried out, can carry out positive and negative two -way flushing to chromatographic column body, improves cleaning efficiency, reduces the disassembly cleaning frequency to chromatographic column body, provides the convenience for use, improves cleaning efficiency.

Description

Technical Field

[0001] This utility model relates to the field of chromatography column technology, specifically to a chromatography column backwashing device. Background Technology

[0002] After a period of use, impurities accumulate inside a chromatographic column, making them difficult to elute. This impurities can affect the column's separation performance, potentially leading to increased column pressure, uneven baseline, double peaks, and reduced separation efficiency. Cleaning the contaminated column can restore some or even most of its separation capacity.

[0003] Chinese Patent CN220939232U discloses a chromatographic column rinsing device, including a base, a rotating frame, and an adjusting frame. Support columns are provided on both sides of the base. The rotating frame is rotatably mounted between the two support columns. The rotating frame has a first collection chamber and a first guide tube communicating with the first collection chamber. The adjusting frame is mounted on the rotating frame and can be operably adjusted to move closer to or further away from the rotating frame. The adjusting frame has a second collection chamber and a second guide tube communicating with the second collection chamber. Several mounting components are provided on the adjusting frame and the rotating frame for mounting the chromatographic column, so that the chromatographic column is connected to the first and second collection chambers. This invention not only greatly improves the cleaning effect of the chromatographic column but also increases the cleaning efficiency.

[0004] However, the above-mentioned disclosed solutions have the following shortcomings: when using the above-mentioned column rinsing device, the column needs to be disassembled from the equipment for cleaning, which causes inconvenience and reduces cleaning efficiency. Utility Model Content

[0005] The purpose of this invention is to address the problems existing in the background technology by proposing a chromatographic column backwashing device.

[0006] The technical solution of this utility model is as follows: A chromatographic column backwashing device includes a chromatographic column, with an adjusting seat at both its input and output ends. A first connecting pipe, a second connecting pipe, and a third connecting pipe are connected to the adjusting seat. The angle between the first and third connecting pipes and the angle between the third and second connecting pipes are both set to 90 degrees. An adjusting mechanism consists of an adjusting component mounted on the adjusting seat and two sets of sealing seats mounted on the adjusting component. The angle between the two sets of sealing seats is set to 90 degrees. The two sets of sealing seats are driven by the adjusting component to rotate and adjust along the inner cavity of the adjusting seat. The sealing seats abut against the side wall of the adjusting seat. A storage tank is also included, containing a cleaning agent chamber and a waste liquid chamber. The output end of the cleaning agent chamber is connected to a three-way valve via a pump. The two output ends of the three-way valve are respectively connected to the second connecting pipe at the input end of the chromatographic column and the third connecting pipe at the output end of the chromatographic column. The third connecting pipe at the input end of the chromatographic column is connected to the waste liquid chamber, and the second connecting pipe at the output end of the chromatographic column is connected to the waste liquid chamber.

[0007] Preferably, an extension tube is connected to the adjusting seat, and an end cap is connected to the end of the extension tube away from the adjusting seat. The end cap is threaded to the end of the chromatographic column.

[0008] Preferably, the first connecting pipe at the input end of the chromatographic column is configured as the column feed pipe, and the first connecting pipe at the output end of the chromatographic column is configured as the column discharge pipe; the second connecting pipe at the input end of the chromatographic column is configured as the forward feed pipe for the cleaning agent, and the second connecting pipe at the output end of the chromatographic column is configured as the forward discharge pipe for the cleaning agent; the third connecting pipe at the input end of the chromatographic column is configured as the reverse discharge pipe for the cleaning agent, and the third connecting pipe at the output end of the chromatographic column is configured as the reverse feed pipe for the cleaning agent.

[0009] Preferably, the adjusting assembly includes a rotating rod rotatably connected to an adjusting seat, one end of the rotating rod extending to the outside of the adjusting seat and mounted on a rotating plate, and the other end of the rotating rod extending into the inner cavity of the adjusting seat and connected to a connecting frame, with two sets of sealing seats mounted on the connecting frame; and two sets of first marking lines, the two sets of first marking lines being set at the top of the rotating plate, the two sets of first marking lines being vertically arranged, the first marking lines corresponding to the sealing seats, and two sets of second marking lines being set at the top of the rotating plate, the two sets of second marking lines being vertically arranged, the second marking lines being located on the same horizontal line as the first marking lines.

[0010] Preferably, the adjustment assembly further includes an abutment seat, which is slidably sleeved on the outside of the rotating plate along the height direction of the rotating plate, and an elastic element is provided between the rotating plate and the abutment seat; and a handle, which is provided in two sets, the two sets of handles being symmetrically installed at the top edge of the abutment seat, and the handles being L-shaped.

[0011] Preferably, the abutment seat is a ring seat, and an anti-slip pad is installed at the bottom of the abutment seat. The anti-slip pad is a ring, which increases the friction at the abutment seat and the adjusting seat.

[0012] Compared with the prior art, the above-mentioned technical solution of this utility model has the following beneficial technical effects: By setting adjustment mechanisms on two sets of adjustment seats, this utility model facilitates adjustment, enables bidirectional rinsing of the chromatographic column, improves cleaning efficiency, reduces the frequency of disassembly and cleaning of the chromatographic column, provides convenience for use, and improves cleaning efficiency. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a schematic diagram showing the connection between the adjustment mechanism and the adjustment seat of this utility model;

[0015] Figure 3 This is a schematic diagram of the adjusting seat structure of this utility model;

[0016] Figure 4 This is a schematic diagram of the adjustment mechanism of this utility model.

[0017] Reference numerals: 1. Chromatographic column; 101. End cap; 2. Adjustment seat; 201. Extension tube; 202. First connecting tube; 203. Second connecting tube; 204. Third connecting tube; 3. Cleaning agent chamber; 301. Three-way valve; 302. Waste liquid chamber; 4. Rotating plate; 401. First marking line; 402. Second marking line; 403. Rotating rod; 404. Connecting frame; 405. Sealing seat; 5. Abutment seat; 501. Handle; 502. Elastic element. Detailed Implementation

[0018] Example 1

[0019] like Figures 1 to 4 As shown, the present invention proposes a chromatographic column backwashing device, comprising a chromatographic column 1, an adjustment mechanism, and a storage tank; both the input and output ends of the chromatographic column 1 are provided with adjustment seats 2, and the adjustment seats 2 are connected to a first connecting pipe 202, a second connecting pipe 203, and a third connecting pipe 204, the included angles between the first connecting pipe 202 and the third connecting pipe 204 and between the third connecting pipe 204 and the second connecting pipe 203 are both set to 90 degrees; the adjustment mechanism consists of an adjustment component provided on the adjustment seat 2 and two sets of sealing seats 405 installed on the adjustment component, the included angle between the two sets of sealing seats 405 is set to 90 degrees, and the two sets of sealing seats 405 are... The plug 405 is driven by the adjustment component to rotate and adjust along the inner cavity of the adjustment seat 2, and the plug 405 is in contact with the side wall of the adjustment seat 2. The storage tank is provided with a cleaning agent chamber 3 and a waste liquid chamber 302. The output end of the cleaning agent chamber 3 is connected to a three-way valve 301 through a liquid pump. The two sets of output ends of the three-way valve 301 are respectively connected to the second connecting pipe 203 at the input end of the chromatographic column 1 and the third connecting pipe 204 at the output end of the chromatographic column 1 through a hose. The third connecting pipe 204 at the input end of the chromatographic column 1 is connected to the waste liquid chamber 302 through a hose, and the second connecting pipe 203 at the output end of the chromatographic column 1 is connected to the waste liquid chamber 302 through a hose.

[0020] Furthermore, an extension tube 201 is connected to the adjusting seat 2, and an end cap 101 is connected to the end of the extension tube 201 away from the adjusting seat 2. The end cap 101 is threadedly connected to the end of the chromatographic column 1. The inner wall of the end cap 101 and the outer wall of the chromatographic column 1 are provided with corresponding threaded portions. A sealing ring corresponding to the end of the chromatographic column 1 is provided inside the end cap 101 to improve the sealing of the connection and prevent leakage.

[0021] The end cap 101 is detachably connected to the chromatographic column 1, allowing for regular and thorough cleaning of the inside of the chromatographic column 1 to avoid interfering with subsequent use.

[0022] Furthermore, the first connecting pipe 202 at the input end of the chromatographic column 1 is configured as the column feed pipe, and the first connecting pipe 202 at the output end of the chromatographic column 1 is configured as the column discharge pipe; the second connecting pipe 203 at the input end of the chromatographic column 1 is configured as the forward feed pipe for the cleaning agent, and the second connecting pipe 203 at the output end of the chromatographic column 1 is configured as the forward discharge pipe for the cleaning agent; the third connecting pipe 204 at the input end of the chromatographic column 1 is configured as the reverse discharge pipe for the cleaning agent, and the third connecting pipe 204 at the output end of the chromatographic column 1 is configured as the reverse feed pipe for the cleaning agent.

[0023] Furthermore, the adjustment assembly includes a rotating rod 403 and a first marking line 401; the rotating rod 403 is rotatably connected to the adjustment seat 2, one end of the rotating rod 403 extends to the outside of the adjustment seat 2 and is mounted on a rotating plate 4, and the other end of the rotating rod 403 extends into the inner cavity of the adjustment seat 2 and is connected to a connecting frame 404, two sets of sealing seats 405 are mounted on the connecting frame 404, and the abutting end of the sealing seat 405 is provided with a sealing gasket to improve the sealing performance between the sealing seat 405 and the opening on the side wall of the adjustment seat 2 and prevent leakage; two sets of the first marking line 401 are provided, the two sets of the first marking line 401 are set at the top of the rotating plate 4, the two sets of the first marking line 401 are set vertically, the first marking line 401 corresponds to the sealing seat 405, and the top of the rotating plate 4 is also provided with two sets of second marking lines 402, the two sets of second marking lines 402 are set vertically, and the second marking lines 402 are set on the same horizontal line as the first marking lines 401.

[0024] In this embodiment, initially, the two sets of sealing seats 405 in the inner cavity of the adjusting seat 2 seal the openings of the third connecting pipe 204 and the second connecting pipe 203, respectively. Material is introduced into the chromatographic column 1 through the first connecting pipe 202 at the input end of the chromatographic column 1 for chromatographic separation. After the chromatographic separation is completed, the material is discharged from the first connecting pipe 202 at the output end of the chromatographic column 1. When rinsing the chromatographic column 1 is required, the rotating plate 4 is rotated, and the rotation direction of the first marker line 401 is observed. This causes the rotating plate 4 to drive the rotating rod 403 to rotate, rotating the two sets of sealing seats 405 along the inner cavity of the adjusting seat 2 until the two sets of sealing seats 405 seal the openings of the first connecting pipe 202 and the third connecting pipe 204, respectively. The cleaning agent inside the cleaning agent chamber 3 is then extracted by the pump and transported to the chromatographic column 1 via the three-way valve 301. In the second connecting pipe 203 at the input end of the column 1, the cleaning agent enters the regulating seat 2 through the second connecting pipe 203 and enters the column 1 along the extension pipe 201 to perform forward flushing of the inside of the column 1. The flushing waste liquid is discharged into the waste liquid chamber 302 along the second connecting pipe 203 at the output end of the column 1. Then, the cleaning agent in the cleaning agent chamber 3 is extracted by the pump and delivered to the third connecting pipe 204 at the output end of the column 1 through the three-way valve 301. The cleaning agent enters the inside of the column 1 through the third connecting pipe 204 to perform reverse flushing of the inside. The flushing waste liquid is discharged into the waste liquid chamber 302 along the third connecting pipe 204 at the input end of the column 1. The column 1 can be flushed in both forward and reverse directions without disassembling it from the equipment, reducing the frequency of disassembly of the column 1 and making cleaning more convenient and faster.

[0025] Example 2

[0026] like Figure 2 and Figure 4 As shown, the chromatographic column backwashing device proposed in this utility model, compared with Embodiment 1, includes an adjustment component that further includes an abutment seat 5 and a handle 501. The abutment seat 5 is slidably sleeved on the outside of the rotating plate 4 along the height direction of the rotating plate 4. A guide strip is provided on the outer wall of the rotating plate 4 and above the adjustment seat 2. A guide groove is provided on the abutment seat 5. The guide strip and the guide groove are slidably connected, so that the abutment seat 5 can only slide and adjust along the height direction of the rotating plate 4. An elastic element 502 is provided between the rotating plate 4 and the abutment seat 5. The elastic element 502 is a spring. Two sets of handles 501 are provided. The two sets of handles 501 are symmetrically installed at the top edge of the abutment seat 5. The handles 501 are L-shaped.

[0027] Furthermore, the abutment seat 5 is designed as an annular seat, and an anti-slip pad is installed at the bottom of the abutment seat 5. The anti-slip pad is an annular ring, which increases the friction between the abutment seat 5 and the adjusting seat 2 and prevents the rotating rod 403 from rotating under non-external force adjustment.

[0028] In this embodiment, when it is necessary to adjust the rotating plate 4 and the rotating rod 403 to rotate along the adjusting seat 2, the two sets of handles 501 are held to drive the abutment seat 5 to slide along the height direction of the rotating plate 4, so that the elastic element 502 is compressed, and the catheter abutment seat 5 moves away from the adjusting seat 2. At the same time, the rotating plate 4 is rotated to drive the rotating rod 403 to rotate along the adjusting seat 2, which drives the connecting frame 404 to rotate, and then drives the two sets of sealing seats 405 to rotate to seal the required opening on the adjusting seat 2. When the handles 501 are released, the elastic element 502 returns to its original position, causing the abutment seat 5 to press against the adjusting seat 2, thereby limiting the rotating rod 403 relative to the adjusting seat 2, preventing it from easily rotating under non-external force adjustment, interfering with the sealing of the opening by the sealing seat 405, and improving the sealing stability of the sealing seat 405.

[0029] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited thereto. Various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention.

Claims

1. A chromatographic column backwashing device, characterized in that, include The chromatographic column (1) is equipped with an adjustment seat (2) at both its input and output ends. The adjustment seat (2) is connected to a first connecting tube (202), a second connecting tube (203), and a third connecting tube (204). The angle between the first connecting tube (202) and the third connecting tube (204) and the angle between the third connecting tube (204) and the second connecting tube (203) are both set to 90 degrees. The adjustment mechanism consists of an adjustment component set on the adjustment seat (2) and two sets of sealing seats (405) installed on the adjustment component. The included angle between the two sets of sealing seats (405) is set to 90 degrees. The two sets of sealing seats (405) are driven by the adjustment component to rotate and adjust along the inner cavity of the adjustment seat (2). The sealing seats (405) are in contact with the side wall of the adjustment seat (2). The container includes a cleaning agent chamber (3) and a waste liquid chamber (302). The output end of the cleaning agent chamber (3) is connected to a three-way valve (301) via a pump. The two output ends of the three-way valve (301) are connected to the second connecting pipe (203) at the input end of the chromatographic column (1) and the third connecting pipe (204) at the output end of the chromatographic column (1), respectively. The third connecting pipe (204) at the input end of the chromatographic column (1) is connected to the waste liquid chamber (302), and the second connecting pipe (203) at the output end of the chromatographic column (1) is connected to the waste liquid chamber (302).

2. The chromatographic column backwashing device according to claim 1, characterized in that, An extension tube (201) is connected to the adjustment seat (2), and an end cap (101) is connected to the end of the extension tube (201) away from the adjustment seat (2). The end cap (101) is threaded to the end of the chromatographic column (1).

3. The chromatographic column backwashing device according to claim 1, characterized in that, The first connecting pipe (202) at the input end of the chromatographic column (1) is set as the column feed pipe, and the first connecting pipe (202) at the output end of the chromatographic column (1) is set as the column discharge pipe; the second connecting pipe (203) at the input end of the chromatographic column (1) is set as the cleaning agent forward feed pipe, and the second connecting pipe (203) at the output end of the chromatographic column (1) is set as the cleaning agent forward discharge pipe; the third connecting pipe (204) at the input end of the chromatographic column (1) is set as the cleaning agent reverse discharge pipe, and the third connecting pipe (204) at the output end of the chromatographic column (1) is set as the cleaning agent reverse feed pipe.

4. The chromatographic column backwashing device according to claim 1, characterized in that, Adjustment components include Rotating rod (403) is rotatably connected to adjusting seat (2). One end of rotating rod (403) extends to the outside of adjusting seat (2) and is equipped with rotating plate (4). The other end of rotating rod (403) extends to the inner cavity of adjusting seat (2) and is connected to connecting frame (404). Two sets of sealing seats (405) are installed on connecting frame (404). And the first marking line (401) is provided in two sets. The two sets of first marking lines (401) are set at the top of the rotating plate (4). The two sets of first marking lines (401) are set vertically. The first marking line (401) corresponds to the sealing seat (405). The top of the rotating plate (4) is also provided with two sets of second marking lines (402). The two sets of second marking lines (402) are set vertically. The second marking lines (402) and the first marking lines (401) are set on the same horizontal line.

5. A chromatographic column backwashing device according to claim 4, characterized in that, The adjustment components also include The abutment seat (5) is slidably sleeved on the outside of the rotating plate (4) along the height direction of the rotating plate (4), and an elastic element (502) is provided between the rotating plate (4) and the abutment seat (5); And handles (501), which are provided in two sets. The two sets of handles (501) are symmetrically installed at the top edge of the abutment seat (5). The handles (501) are L-shaped.

6. A column backwashing device according to claim 5, characterized in that, The abutment seat (5) is set as an annular seat, and an anti-slip pad is installed at the bottom of the abutment seat (5). The anti-slip pad is set as an annular ring to increase the friction at the abutment seat (5) and the adjusting seat (2).

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