A glass slide bonding and liquid injection fixture

Abstract

This utility model provides a slide-fitting and liquid-filling fixture, including a slide assembly and a clamping assembly. The slide assembly includes an upper slide, a lower slide, and a flexible pad. The flexible pad is disposed between the upper slide and the lower slide to form a flow channel between them. The upper slide has an inlet and an outlet, which are respectively connected to the flow channel. The clamping assembly includes an upper cover, a lower cover, and an elastic compression member. The elastic compression member is disposed on the upper cover. The upper slide, the flexible pad, and the lower slide are clamped between the elastic compression member and the lower cover in a top-to-bottom arrangement, effectively reducing reagent usage while ensuring sufficient contact between the reagent and the sample.

Description

Technical Field

[0001] This utility model relates to the field of glass slide technology, and in particular to a glass slide bonding and liquid injection tool. Background Technology

[0002] In laboratory operations, adding samples to glass slides is a common experimental procedure. Currently, laboratory operators generally add sufficient reagent directly to the sample on the slide to ensure that the reagent completely submerges the sample, thereby achieving sufficient contact between the reagent and the sample and meeting the requirements of subsequent experimental analysis.

[0003] However, the existing sample addition method has obvious drawbacks. The reagents used in related experiments are often quite precious, costly, and their acquisition process can be complex. Adding sufficient reagent directly to submerge the sample leads to significant reagent waste, increases experimental costs, and hinders the rational use of resources.

[0004] At the same time, ensuring sufficient contact between reagents and samples while conserving reagents becomes a major challenge. Reducing the amount of reagent added makes it difficult to ensure that the reagents fully cover the samples, which may result in some samples not coming into contact with the reagents, thus affecting the accuracy and reliability of the experimental results. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by providing a slide-fitting and liquid-dispensing fixture that can save reagents while ensuring full contact between the reagents and the sample.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A glass slide bonding and liquid injection fixture, comprising:

[0008] A glass slide assembly, comprising an upper glass slide, a lower glass slide, and a flexible pad, wherein the flexible pad is disposed between the upper glass slide and the lower glass slide to form a flow channel between the upper glass slide and the lower glass slide, and the upper glass slide has an inlet and an outlet, the inlet and the outlet being respectively connected to the flow channel;

[0009] A clamping assembly includes an upper cover, a lower cover, and an elastic compression member disposed on the upper cover. The upper slide, a flexible pad, and a lower slide are clamped between the elastic compression member and the lower cover in a top-to-bottom arrangement.

[0010] In a preferred embodiment, the inlet and outlet are located at both ends of the flow channel along its length.

[0011] In a preferred embodiment, the thickness of the flexible pad is less than the thickness of the upper glass slide and the lower glass slide, respectively.

[0012] In a preferred embodiment, the lower cover includes:

[0013] The lower cover base plate is attached to the lower glass sheet;

[0014] The lower cover support block is provided on both sides of the lower cover bottom plate in the width direction, and the upper cover abuts against the lower cover support block.

[0015] In a preferred embodiment, the download glass slide is completely fitted to the bottom cover plate.

[0016] In a preferred embodiment, a lower observation port is provided on the bottom plate of the lower cover.

[0017] In a preferred embodiment, the elastic compression member includes:

[0018] A support rod, which passes through the upper cover;

[0019] A support plate, which is connected to the lower end of the support rod, and abuts against the upper glass slide;

[0020] A spring is sleeved on the support rod and located between the upper cover and the support plate.

[0021] In a preferred embodiment, there are multiple elastic compression members, which are evenly arranged on the upper cover.

[0022] In a preferred embodiment, the upper cover has an upper observation port, and a plurality of elastic compression members surround the upper observation port.

[0023] Compared with existing technologies, this technical solution has the following advantages:

[0024] The flow channel is used to contain the sample. After the reagent is injected through the inlet, it flows along the flow channel until it flows out from the outlet. When reagent flows out of the outlet, it indicates that the flow channel is full of reagent and the reagent is in sufficient contact with the sample. At this point, the injection of reagent into the inlet can be stopped. Compared with the existing reagent dripping method, this method effectively reduces the amount of reagent used while ensuring sufficient contact between the reagent and the sample.

[0025] The slide assembly is held in place by the elastic compression member and the lower cover, so that the flexible pad fits tightly between the upper and lower slides to prevent reagent leakage.

[0026] The flexible pad is relatively thin, which results in a smaller flow volume. This effectively reduces the amount of reagents used and lowers experimental costs while still meeting experimental requirements.

[0027] The flow channel is observed through the upper and lower observation ports to ensure the reliability of the experiment. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the structure of the glass slide bonding and liquid injection fixture described in this utility model;

[0029] Figure 2 for Figure 1 Enlarged diagram of A in the middle;

[0030] Figure 3 This is a perspective view of the glass slide bonding and liquid injection fixture described in this utility model;

[0031] Figure 4 This is a front view of the glass slide bonding and liquid injection fixture described in this utility model;

[0032] Figure 5 for Figure 4 A sectional view along the BB direction;

[0033] Figure 6 This is a top view of the glass slide bonding and liquid injection fixture described in this utility model.

[0034] In the diagram: 100 glass slide assembly, 100a flow channel, 100b liquid inlet, 100c liquid outlet, 110 upper glass slide, 120 lower glass slide, 130 flexible pad, 200 clamp assembly, 210 upper cover, 210a upper observation port, 220 lower cover, 221 lower cover base plate, 221a lower observation port, 222 lower cover support block, 230 elastic compression component, 231 support rod, 232 support plate. Detailed Implementation

[0035] The following description is intended to disclose the present invention so that those skilled in the art can implement it. The preferred embodiments described below are merely examples, and other obvious variations will occur to those skilled in the art. The basic principles of the present invention defined in the following description can be applied to other embodiments, modifications, improvements, equivalents, and other technical solutions that do not depart from the spirit and scope of the present invention.

[0036] In existing technologies, the reaction between samples and reagents on a glass slide is usually achieved by directly adding reagents. This requires adding an excessive amount of reagent to ensure complete coverage of the sample, resulting in a large amount of reagent used. If the amount of reagent added is reduced, it is difficult to ensure that the reagent can fully cover the sample, which may result in some samples not being able to come into contact with the reagent, thereby affecting the accuracy and reliability of the experimental results.

[0037] Please refer to Figures 1 to 5 This utility model provides a slide-mounted liquid dispensing fixture to solve the problems existing in the prior art, ensuring sufficient contact between the reagent and the sample while saving reagents, including:

[0038] A slide assembly 100 includes an upper slide 110, a lower slide 120, and a flexible pad 130. The flexible pad 130 is disposed between the upper slide 110 and the lower slide 120 to form a flow channel 100a between the upper slide 110 and the lower slide 120. The upper slide 110 has a liquid inlet 100b and a liquid outlet 100c, which are respectively connected to the flow channel 100a.

[0039] The clamp assembly 200 includes an upper cover 210, a lower cover 220, and an elastic compression member 230. The elastic compression member 230 is disposed on the upper cover 210. The upper slide 110, the flexible pad 130, and the lower slide 120 are clamped between the elastic compression member 230 and the lower cover 220 in a top-to-bottom arrangement.

[0040] The flow channel 100a is used to contain the sample. After the reagent is injected through the inlet 100b, it flows along the flow channel 100a until it flows out from the outlet 100c. When reagent flows out of the outlet 100c, it indicates that the flow channel 100a is full of reagent and the reagent is in full contact with the sample. At this point, the injection of reagent into the inlet 100b can be stopped. Compared with the existing reagent drop method, this method effectively reduces the amount of reagent used while ensuring full contact between the reagent and the sample. The slide assembly 100 is clamped by the elastic compression member 230 and the lower cover 220, so that the flexible pad 130 is tightly attached between the upper slide 110 and the lower slide 120 to prevent reagent leakage.

[0041] The samples include blood, urine, saliva, cells, genes, biological slides, etc., and the reagents include biochemical, immunological, cell culture, and molecular biology reagents.

[0042] like Figure 1 and Figure 2As shown, both the upper slide 110 and the lower slide 120 are rectangular and have the same dimensions. The upper slide 110, the elastic compression member 230, and the lower slide 120 are aligned along their length and then stacked sequentially from top to bottom. Both the upper slide 110 and the lower slide 120 are made of transparent material, allowing researchers to easily observe the internal flow channels 100a, such as whether they are filled with reagents, thus enabling them to monitor the experimental progress.

[0043] like Figure 5 As shown, the flow channel 100a is elongated, and its direction is consistent with the length direction of the upper slide 110 and the lower slide 120. The inlet 100b and the outlet 100c are located at opposite ends of the length direction of the flow channel 100a. This allows the reagent injected through the inlet 100b to flow along the length direction of the flow channel 100a until it is discharged through the outlet 100c, ensuring sufficient time and space for the sample and reagent to make adequate contact, thus laying the foundation for accurate subsequent experimental analysis.

[0044] The left and right ends of the flow channel 100a are each triangular, with the apex of the triangle facing outwards. The liquid inlet 100b is connected to the apex of the right triangle of the flow channel 100a, and the liquid outlet 100c is connected to the apex of the left triangle of the flow channel 100a.

[0045] The reagent introduced from the inlet 100b enters from the apex of the triangle at the right end of the flow channel 100a. Under the guiding effect of the triangle, the reagent can pass through the flow channel 100a smoothly and steadily, and then reach the triangular area at the left end of the flow channel 100a. This triangle also plays a guiding role, guiding the reagent to be smoothly discharged from the outlet 100c connected to its apex, further optimizing the flow path of the reagent and ensuring that the reagent and sample are fully mixed and reacted.

[0046] The flexible pad 130 may be made of silicone. After the slide assembly 100 is pressed between the elastic compression member 230 and the lower cover 220, the flexible pad 130 not only forms the flow channel 100a, but also ensures that the flow channel 100a has good sealing performance.

[0047] like Figure 2 As shown, the thickness of the flexible pad 130 is less than the thickness of the upper slide 110 and the lower slide 120, respectively. That is, the flexible pad 130 is thinner. This design makes the volume of the flow 100a relatively small, which effectively reduces the amount of reagents used and lowers the experimental cost while meeting the experimental requirements.

[0048] refer to Figure 1 and Figure 5The number of flow channels 100a can be two, and they are arranged at intervals along the width direction of the flexible pad 130. At this time, the upper glass slide 110 has two liquid inlets 100b and two liquid outlets 100c, that is, each flow channel 100a is independently connected to one liquid inlet 100b and one liquid outlet 100c.

[0049] Since the two flow channels 100a are separated and do not interfere with each other, this design allows each flow channel 100a to form a relatively independent reaction space.

[0050] like Figure 1 and Figure 3 As shown, the lower cover 220 includes:

[0051] The lower cover base plate 221 is attached to the lower glass slide 120;

[0052] The lower cover support block 222 is provided on both sides of the lower cover bottom plate 221 in the width direction, and the upper cover 210 abuts against the lower cover support block 222.

[0053] The lower cover plate 221 and the download glass slide 120 have the same shape, both being rectangular. Furthermore, the size of the lower cover plate 221 is not smaller than that of the download glass slide 120, so that the lower surface of the download glass slide 120 completely adheres to the upper surface of the lower cover plate 221, preventing the download glass slide 120 from partially suspending and cracking when the elastic compression member 230 presses the glass slide assembly 100.

[0054] The lower cover support block 222 is integrally formed with the lower cover base plate 221. The upper surface of the lower cover support block 222 abuts against the upper cover 210, so that there is a gap between the upper cover 210 and the lower cover base plate 221 for arranging the glass slide assembly 100. The lower cover support block 222 and the upper cover 210 can be fixed together by bolts.

[0055] like Figure 1 As shown, the elastic compression member 230 includes:

[0056] Support rod 231, the support rod 231 passing through the upper cover 210;

[0057] Support plate 232, the support plate 232 is connected to the lower end of the support rod 231, and the support plate 232 abuts against the upper glass slide 110;

[0058] A spring is sleeved on the support rod 231 and located between the upper cover 210 and the support plate 232.

[0059] The cross-sectional area of ​​the support plate 232 is larger than that of the support rod 231. This not only increases the contact area between the support plate 232 and the upper glass slide 110, making the pressure distribution more uniform, but also serves to cooperate with the spring. Under the action of the spring, the support plate 232 is tightly fitted to the upper glass slide 110, thereby ensuring a tight fit between the flexible pad 130 and both the upper and lower glass slides 110, forming a reliable sealed flow channel 100a to prevent reagent leakage from affecting the experimental results.

[0060] The spring force satisfies the pressure of the support plate 232 against the upper glass slide 110, so that the glass slide assembly 100 is tightly pressed between the support plate 232 and the lower cover plate 221.

[0061] A nut can be locked onto the support rod 231, with the nut positioned above the upper cover 210 and the spring positioned below the upper cover 210, to secure the elastic compression member 230 to the upper cover 210. This allows the elastic compression member 230 and the upper cover 210 to be assembled as a single unit with the lower cover 220. The distance between the support plate 232 and the upper cover 210 can be adjusted by rotating the nut clockwise or counterclockwise, thereby adjusting the spring force to accommodate the pressure requirements of different experiments.

[0062] The support plate 232 may be made of rubber, and the support plate 232 is partially staggered from the flow channel 100a to prevent damage to the upper glass slide 110.

[0063] The number of elastic compression members 230 is multiple, and they are evenly arranged on the upper cover 210. This makes the pressing force more uniform, ensures that the forces on each part of the slide assembly 100 are balanced, and further guarantees the sealing effect between the flexible pad 130 and the upper slide 110 and the lower slide 120.

[0064] like Figure 1 and 6 As shown, the upper cover 210 has an upper observation port 210a, and multiple elastic compression members 230 surround the upper observation port 210a. Since both the upper slide 110 and the lower slide 120 are made of transparent material, the condition of their internal flow channels 100a can be observed through the upper observation port 210a, such as whether the flow channels 100a are filled with reagents, allowing the experimenter to monitor the experimental progress in a timely manner.

[0065] like Figure 3 As shown, a lower observation port 221a is provided on the bottom plate 221 of the lower cover. This provides convenience for the experimenters to observe the situation inside the flow channel 100a from below, further enhancing the observability of the experimental device.

[0066] The method of using the glass slide bonding and liquid injection fixture is as follows:

[0067] First, place the flexible pad 130 on the download slide 120, then place the sample in the area enclosed by the flexible pad 130, and then place the upper slide 110 on the flexible pad 130, so that they form the slide assembly 100.

[0068] Next, the slide assembly 100 is placed on the bottom plate 221 of the lower cover 220, and the upper cover 210 is put on. The upper cover 210 and the lower cover support block 222 are fixed by bolts. At this time, the elastic compression member 230 applies pressure to the slide assembly 100, so that the flexible pad 130 is tightly attached between the upper slide 110 and the lower slide 120, forming a sealed flow channel 100a.

[0069] Then, the reagent is injected into the flow channel 100a through the inlet 100b. The reagent flows along the flow channel 100a until it flows out from the outlet 100c. At this point, the injection of reagent is stopped, and the reagent is in full contact with the sample, so that subsequent experimental analysis can be carried out.

[0070] During the experiment, the flow channel 100a can be observed through the upper observation port 210a and the lower observation port 221a.

[0071] In summary, the flow channel 100a is used to contain the sample. After the reagent is injected through the inlet 100b, it flows along the flow channel 100a until it flows out from the outlet 100c. When reagent flows out of the outlet 100c, it indicates that the flow channel 100a is full of reagent and the reagent is in full contact with the sample. At this point, the injection of reagent into the inlet 100b can be stopped. Compared with the existing reagent drop method, this method effectively reduces the amount of reagent used while ensuring full contact between the reagent and the sample. The slide assembly 100 is clamped by the elastic compression member 230 and the lower cover 220, so that the flexible pad 130 is tightly attached between the upper slide 110 and the lower slide 120 to prevent reagent leakage.

[0072] The embodiments described above are only used to illustrate the technical ideas and features of this utility model. Their purpose is to enable those skilled in the art to understand the content of this utility model and implement it accordingly. The scope of patent application of this utility model should not be limited by these embodiments. That is, any equivalent changes or modifications made in accordance with the spirit disclosed in this utility model still fall within the patent scope of this utility model.

Claims

1. A glass slide lamination liquid injection tool, characterized by, include: A slide assembly (100) includes an upper slide (110), a lower slide (120), and a flexible pad (130). The flexible pad (130) is disposed between the upper slide (110) and the lower slide (120) to form a flow channel (100a) between the upper slide (110) and the lower slide (120). The upper slide (110) has an inlet (100b) and an outlet (100c), which are respectively connected to the flow channel (100a). The clamp assembly (200) includes an upper cover (210), a lower cover (220), and an elastic compression member (230). The elastic compression member (230) is disposed on the upper cover (210), and the upper slide (110), the flexible pad (130), and the lower slide (120) are clamped between the elastic compression member (230) and the lower cover (220) in a top-to-bottom arrangement.

2. The glass slide bonding and liquid injection fixture as described in claim 1, characterized in that, The inlet (100b) and outlet (100c) are located at both ends of the length of the flow channel (100a).

3. The glass slide bonding and liquid injection fixture as described in claim 1, characterized in that, The thickness of the flexible pad (130) is less than the thickness of the upper glass slide (110) and the lower glass slide (120), respectively.

4. The glass slide bonding and liquid injection fixture as described in claim 1, characterized in that, The lower cover (220) includes: The lower cover base plate (221) is attached to the lower glass slide (120); The lower cover support block (222) is provided on both sides of the lower cover bottom plate (221) in the width direction, and the upper cover (210) abuts against the lower cover support block (222).

5. The glass slide bonding and liquid injection fixture as described in claim 4, characterized in that, The download glass slide (120) is completely attached to the bottom cover plate (221).

6. The glass slide bonding and liquid injection fixture as described in claim 4, characterized in that, The bottom plate (221) of the lower cover is provided with a lower observation port (221a).

7. The glass slide bonding and liquid injection fixture as described in claim 1, characterized in that, The elastic compression member (230) includes: A support rod (231) passes through the upper cover (210). A support plate (232) is connected to the lower end of the support rod (231), and the support plate (232) abuts against the upper glass slide (110); A spring is sleeved on the support rod (231) and located between the upper cover (210) and the support plate (232).

8. The glass slide bonding and liquid injection fixture as described in claim 7, characterized in that, The number of elastic compression members (230) is multiple, and they are evenly arranged on the upper cover (210).

9. The glass slide bonding and liquid injection fixture as described in claim 8, characterized in that, The upper cover (210) has an upper observation port (210a), and a plurality of elastic compression members (230) surround the upper observation port (210a).

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