Phototrophic bacteria group amplification culture device

Abstract

The utility model relates to photosynthetic bacteria group culture equipment technical field discloses photosynthetic bacteria group expansion culture device, including culture jar, the outer wall bottom of culture jar is fixedly connected with expansion mechanism, the expansion mechanism is used for expanding culture bacteria group, the inner wall middle part of expansion mechanism is rotatably connected with feeding mechanism, the feeding mechanism is used for stirring bacteria group, the outer wall top of culture jar is fixedly connected with control mechanism, the expansion mechanism includes expansion jar, the expansion jar fixedly connected in the outer wall bottom of culture jar, the inner wall top of expansion jar is fixedly connected with the connecting cover, the outer wall top of connecting cover is equipped with the feeding hole, in the utility model, the bacteria group growth in culture jar, need to expand the culture when entering expansion jar through the feeding hole of connecting cover, and connecting frame fixed support supplies light, and a large number of supply mode real -time response bacteria group metabolic change, improve photosynthetic bacteria group culture's stability and product yield.

Description

Technical Field

[0001] This utility model relates to the field of photosynthetic bacteria culture equipment, and in particular to a photosynthetic bacteria expansion culture device. Background Technology

[0002] Photosynthetic bacteria are a group of microorganisms capable of photosynthesis. They are a collection of bacteria that can use light energy to convert carbon dioxide into organic matter and release oxygen and other products. Large-scale cultivation of photosynthetic bacteria requires overcoming bottlenecks in strain selection, cultivation costs, and maintaining activity. However, with the advancement of synthetic biology and fermentation engineering, their application scenarios will continue to expand, becoming a potential biological solution to environmental, energy, and food problems.

[0003] Photosynthetic microbial community expansion culture devices are a type of equipment system specifically designed for the large-scale cultivation of photosynthetic microorganisms. Their main function is to achieve high-density growth of photosynthetic microbial communities and accumulation of metabolites by regulating environmental parameters such as light, temperature and nutrient supply. However, in existing photosynthetic microbial community expansion culture devices, the demand for culture materials varies significantly at different growth stages of photosynthetic bacteria. The traditional timed and quantitative supply mode is difficult to respond to changes in microbial community metabolism in real time, resulting in poor stability of photosynthetic microbial community culture and reduced product yield. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides a photosynthetic bacteria expansion culture device, which aims to improve the problem that the quantitative supply mode in the prior art is difficult to respond to changes in bacterial metabolism in real time, resulting in poor stability of photosynthetic bacteria culture and reduced product yield.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a photosynthetic bacteria colony expansion and cultivation device, comprising a culture tank, an expansion mechanism fixedly connected to the bottom of the outer wall of the culture tank, the expansion mechanism being used to expand the cultured bacteria colony, a feeding mechanism rotatably connected to the middle of the inner wall of the expansion mechanism, the feeding mechanism being used to stir the bacteria colony, and a control mechanism fixedly connected to the top of the outer wall of the culture tank; the expansion mechanism comprises an expansion tank, the expansion tank being fixedly connected to the bottom of the outer wall of the culture tank, a connecting cover fixedly connected to the top of the inner wall of the expansion tank, a feeding hole being opened on the top of the outer wall of the connecting cover, a sealing ring being fixedly connected around the outer wall of the connecting cover, and a connecting assembly being fixedly connected around the outer wall of the expansion tank.

[0006] As a further description of the above technical solution:

[0007] The connecting assembly includes a connecting frame, which is fixedly connected to the front and rear sides of the top of the expansion tank. A supplementary light is fixedly connected to the middle of the inner wall of the front connecting frame, and a temperature controller is installed in the middle of the inner wall of the rear connecting frame. Connecting rings are fixedly connected to the left and right sides of the bottom of the expansion tank.

[0008] As a further description of the above technical solution:

[0009] The feeding mechanism includes a feeding pipe that passes through the middle of the inner wall of the expansion tank. A branch pipe is connected to the bottom of the outer wall of the feeding pipe. Multiple spray pipes are fixedly connected to one side of the outer wall of the branch pipe. Multiple fixing buckles are fixedly connected to the upper and lower sides of the outer wall of the branch pipe. A stirring assembly is fixedly connected to the top of the outer wall of the branch pipe.

[0010] As a further description of the above technical solution:

[0011] The stirring assembly includes a motor, which is fixedly connected to the top right side of the culture tank. A bevel gear one is fixedly connected to the output end of the motor. A bevel gear two is rotatably connected to the middle of the top wall of the culture tank. The bevel gear two meshes with the bevel gear one. A connecting shaft is fixedly connected to the bottom of the outer wall of the bevel gear two. The connecting shaft is fixedly connected to the outer wall of the feed pipe. Multiple stirring blades are fixedly connected to the front and rear sides of the outer wall of the connecting shaft.

[0012] As a further description of the above technical solution:

[0013] The control mechanism includes an output pipe that is connected to the top of the outer wall of the culture tank. A fixing frame is fixedly connected around the outer wall of the output pipe, and a flow limiting component is fixedly connected to the rear side of the outer wall of the fixing frame.

[0014] As a further description of the above technical solution:

[0015] The flow limiting component includes a proportional valve, which is fixedly connected to the rear side of the outer wall of the fixing frame, and a valve is installed on the front side of the outer wall of the proportional valve.

[0016] As a further description of the above technical solution:

[0017] A support frame is fixedly connected to the top side of the feed pipe, and a screw is threaded onto the outer wall side of the support frame.

[0018] As a further description of the above technical solution:

[0019] An observation window is fixedly connected to one side of the outer wall of the culture tank, and an exhaust hole is provided at the top of the outer wall of the culture tank.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, when the microbial community in the culture tank needs to be expanded, it enters the expansion tank through the feed hole of the connecting cover. The connecting frame provides fixed support, the supplementary light provides light, the temperature controller controls the temperature, the sealing ring prevents dust and seals, the feeding mechanism stirs to promote the mixing of microbial community and nutrients, the control mechanism adjusts the parameters to ensure smooth culture, and the mass supply mode responds to changes in microbial community metabolism in real time, improving the stability of photosynthetic microbial community culture and product yield.

[0022] 2. In this utility model, when the feeding mechanism is working, the material enters the expansion tank through the feeding pipe, is dispersed and conveyed through the branch pipe and the spray pipe, the motor drives the first bevel gear, and the second bevel gear drives the connecting shaft to rotate, so that the feeding pipe and the branch pipe rotate, the stirring blades stir the material synchronously, and the fixing buckle ensures the stability of the structure. Attached Figure Description

[0023] Figure 1 This is a perspective view of the photosynthetic bacteria colony expansion culture device proposed in this utility model;

[0024] Figure 2 This is a front view of the photosynthetic bacteria colony expansion culture device proposed in this utility model;

[0025] Figure 3 This is a structural exploded view of the photosynthetic bacteria colony expansion culture device proposed in this utility model;

[0026] Figure 4 This is a partial structural exploded view of the photosynthetic bacteria colony expansion culture device proposed in this utility model;

[0027] Figure 5 This is a partial structural schematic diagram of the photosynthetic bacteria colony expansion culture device proposed in this utility model.

[0028] Legend:

[0029] 1. Culture tank; 2. Expansion mechanism; 201. Expansion tank; 202. Connecting cover; 203. Feed port; 204. Sealing ring; 205. Connecting assembly; 2051. Connecting frame; 2052. Supplemental light; 2053. Temperature controller; 2054. Connecting ring; 3. Feeding mechanism; 301. Feed pipe; 302. Branch pipe; 303. Spray pipe; 304. Fixing buckle; 305. Stirring assembly; 3051. Motor; 3052. Bevel gear one; 3053. Bevel gear two; 3054. Connecting shaft; 3055. Stirring blade; 4. Control mechanism; 401. Output pipe; 402. Fixing frame; 403. Flow limiting assembly; 4031. Proportional valve; 4032. Valve; 5. Support frame; 6. Screw; 7. Observation window; 8. Exhaust port. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Reference Figure 1 , Figure 3 and Figure 4 This utility model provides an embodiment of a photosynthetic bacteria colony expansion and cultivation device, comprising a culture tank 1, an expansion mechanism 2 fixedly connected to the bottom of the outer wall of the culture tank 1 for expanding the cultured bacteria, a feeding mechanism 3 rotatably connected to the middle of the inner wall of the expansion mechanism 2 for stirring the bacteria, and a control mechanism 4 fixedly connected to the top of the outer wall of the culture tank 1; the expansion mechanism 2 includes an expansion tank 201 fixedly connected to the bottom of the outer wall of the culture tank 1, and a connecting cover 202 fixedly connected to the top of the inner wall of the expansion tank 201. The top of the outer wall of 02 is provided with a feed hole 203. A sealing ring 204 is fixedly connected to the outer wall of the connecting cover 202. A connecting component 205 is fixedly connected to the outer wall of the expansion tank 201. The connecting component 205 includes a connecting frame 2051. The connecting frame 2051 is fixedly connected to the front and rear sides of the top of the expansion tank 201. A supplementary light 2052 is fixedly connected to the middle of the inner wall of the front connecting frame 2051. A temperature controller 2053 is installed in the middle of the inner wall of the rear connecting frame 2051. A connecting ring 2054 is fixedly connected to the left and right sides of the bottom of the expansion tank 201.

[0032] Specifically, the photosynthetic bacteria grow in culture tank 1. When expanding as needed, the culture medium is supplemented with reagents according to the ratio and enters the expansion tank 201 through the feed hole 203. The expansion tank 201 is fixed and supported by the connecting frame 2051. The front supplemental light 2052 provides light, and the rear temperature controller 2053 regulates the temperature. The sealing ring 204 of the connecting cover 202 prevents impurities. The bottom connecting ring 2054 is used for fixing or connecting. The feeding mechanism 3 stirs to promote the contact of bacteria with nutrients. The control mechanism 4 adjusts the parameters. All equipment needs to be dissolved and sterilized with boiling water. During the cultivation period, the tank is shaken twice a day to ensure successful cultivation.

[0033] Reference Figure 1 , Figure 2 and Figure 5The feeding mechanism 3 includes a feeding pipe 301, which penetrates the middle of the inner wall of the expansion tank 201. A branch pipe 302 is connected to the bottom of the outer wall of the feeding pipe 301. Multiple spray nozzles 303 are fixedly connected to one side of the outer wall of the branch pipe 302. Multiple fixing buckles 304 are fixedly connected to the upper and lower sides of the outer wall of the branch pipe 302. A stirring assembly 305 is fixedly connected to the top of the outer wall of the branch pipe 302. The stirring assembly 305 includes a motor 3051, which is fixedly connected to the feed tank. On the top right side of the culture tank 1, a bevel gear 3052 is fixedly connected to the output end of the motor 3051. A bevel gear 3053 is rotatably connected to the middle of the top wall of the culture tank 1. The bevel gear 3053 meshes with the bevel gear 3052. A connecting shaft 3054 is fixedly connected to the bottom of the outer wall of the bevel gear 3053. The connecting shaft 3054 is fixedly connected to the outer wall of the feed pipe 301. Multiple stirring blades 3055 are fixedly connected to the front and rear sides of the outer wall of the connecting shaft 3054.

[0034] Specifically, the material enters the expansion tank 201 through the feed pipe 301, and part of it is diverted to the branch pipe 302 and dispersed and conveyed by the spray pipe 303. The motor 3051 starts and drives the first bevel gear 3052 to rotate, meshing with the second bevel gear 3053. The power is transmitted through the connecting shaft 3054, causing the feed pipe 301 and the branch pipe 302 to rotate. The stirring blade 3055 stirs the mixture, and the fixing buckle 304 stabilizes the branch pipe 302, ensuring that the material conveying and stirring process is stable and efficient, and achieving uniform dispersion and full mixing.

[0035] Reference Figure 1 , Figure 2 and Figure 3 The control mechanism 4 includes an output pipe 401, which is connected to the top of the outer wall of the culture tank 1. A fixing frame 402 is fixedly connected around the outer wall of the output pipe 401. A flow limiting component 403 is fixedly connected to the rear side of the outer wall of the fixing frame 402. The flow limiting component 403 includes a proportional valve 4031, which is fixedly connected to the rear side of the outer wall of the fixing frame 402. A valve 4032 is installed on the front side of the outer wall of the proportional valve 4031. A support frame 5 is fixedly connected to the top side of the feed pipe 301. A screw 6 is threadedly connected to the outer wall side of the support frame 5. An observation window 7 is fixedly connected to the outer wall side of the culture tank 1. An exhaust hole 8 is opened at the top of the outer wall of the culture tank 1.

[0036] Specifically, the working principle of the control mechanism 4 is as follows: When it is necessary to discharge the material in the culture tank 1, the material flows out through the output pipe 401. The fixing frame 402 plays a role in stabilizing the output pipe 401 and ensuring the stability of its connection. The proportional valve 4031 in the flow limiting component 403 can adjust the opening and closing degree of the valve 4032 according to actual needs, thereby accurately controlling the flow rate of the material. The support frame 5 and screw 6 are used to fix the feed pipe 301 to ensure the stability of the feeding process. The observation window 7 allows the operator to observe the material status in the culture tank 1 in real time, while the exhaust port 8 can discharge the gas in the culture tank 1, maintain the pressure balance in the tank, and ensure the safe and stable operation of the entire system.

[0037] Working principle: When the photosynthetic bacteria expansion culture device is running, the bacteria in culture tank 1 grow under suitable conditions. The required number of layers is stacked according to user needs. When it is necessary to expand the culture scale, the photosynthetic bacteria culture medium is mixed according to the following ratio: 20g sodium acetate, 20g potassium dioxin, 2g magnesium sulfate, 20g ammonium sulfate, 2g sodium oxide, and 1g manganese sulfate. This mixture enters the expansion tank 201 through the feed hole 203 on the connecting cover 202 to achieve bacterial culture. The connecting frames 2051 around the expansion tank 201 provide fixation and support. The supplementary light 2052 on the front connecting frame 2051 provides light to the photosynthetic bacteria to meet their photosynthetic needs; the temperature controller 2053 on the rear connecting frame 2051... The temperature inside the expansion tank 201 is monitored and adjusted in real time to ensure that the bacteria multiply in a suitable environment. The sealing ring 204 on the connecting cover 202 prevents external impurities from entering and ensures a pure culture environment. The connecting ring 2054 at the bottom can be used to fix the device or connect other components. The feeding mechanism 3 rotates and stirs inside the expansion tank 201 to promote full contact between the bacteria and nutrients and accelerate the expansion culture process. The control mechanism 4 regulates relevant parameters during the culture process. It should be noted that the required 10 liters of original bacteria and 10 liters of culture medium need to be dissolved in boiling water. At the same time, all equipment should be sterilized by boiling water. During the culture process, it needs to be shaken twice a day to ensure the smooth progress of the entire culture process.

[0038] When the feeding mechanism 3 is working, the material enters the expansion tank 201 through the feeding pipe 301. Some of the material is diverted to the branch pipe 302 and dispersed and conveyed through the spray pipe 303. After the motor 3051 is started, it drives the first bevel gear 3052 to rotate. Through the meshing transmission with the second bevel gear 3053, the power is transmitted to the connecting shaft 3054, which causes the feeding pipe 301 and the branch pipe 302 to rotate together. At the same time, the stirring blade 3055 on the connecting shaft 3054 rotates to stir and mix the material. The fixing buckle 304 is used to stabilize the position of the branch pipe 302 to ensure that the material conveying and stirring process is stable and efficient, and to achieve uniform dispersion and full mixing of the material.

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A photosynthetic bacteria expansion culture device, comprising a culture tank (1), characterized in that: An expansion mechanism (2) is fixedly connected to the bottom of the outer wall of the culture tank (1). The expansion mechanism (2) is used to expand the cultured microbial community. A feeding mechanism (3) is rotatably connected to the middle of the inner wall of the expansion mechanism (2). The feeding mechanism (3) is used to stir the microbial community. A control mechanism (4) is fixedly connected to the top of the outer wall of the culture tank (1). The expansion mechanism (2) includes an expansion tank (201), which is fixedly connected to the bottom of the outer wall of the culture tank (1). A connecting cover (202) is fixedly connected to the top of the inner wall of the expansion tank (201). A feed hole (203) is opened on the top of the outer wall of the connecting cover (202). A sealing ring (204) is fixedly connected around the outer wall of the connecting cover (202). A connecting assembly (205) is fixedly connected around the outer wall of the expansion tank (201).

2. The photosynthetic bacteria expansion culture device according to claim 1, characterized in that: The connecting assembly (205) includes a connecting frame (2051), which is fixedly connected to the front and rear sides of the top of the expansion tank (201). A supplementary light (2052) is fixedly connected to the middle of the inner wall of the front connecting frame (2051), and a temperature controller (2053) is installed in the middle of the inner wall of the rear connecting frame (2051). Connecting rings (2054) are fixedly connected to the left and right sides of the bottom of the expansion tank (201).

3. The photosynthetic bacteria expansion culture device according to claim 1, characterized in that: The feeding mechanism (3) includes a feeding pipe (301), which passes through the middle of the inner wall of the expansion tank (201). The bottom of the outer wall of the feeding pipe (301) is connected to a branch pipe (302). A plurality of spray pipes (303) are fixedly connected to one side of the outer wall of the branch pipe (302). A plurality of fixing buckles (304) are fixedly connected to the upper and lower sides of the outer wall of the branch pipe (302). A stirring assembly (305) is fixedly connected to the top of the outer wall of the branch pipe (302).

4. The photosynthetic bacteria expansion culture device according to claim 3, characterized in that: The stirring assembly (305) includes a motor (3051), which is fixedly connected to the top right side of the culture tank (1). A bevel gear (3052) is fixedly connected to the output end of the motor (3051). A bevel gear (3053) is rotatably connected to the middle of the top wall of the culture tank (1). The bevel gear (3053) meshes with the bevel gear (3052). A connecting shaft (3054) is fixedly connected to the bottom of the outer wall of the bevel gear (3053). The connecting shaft (3054) is fixedly connected to the outer wall of the feed pipe (301). Multiple stirring blades (3055) are fixedly connected to the front and rear sides of the outer wall of the connecting shaft (3054).

5. The photosynthetic bacteria expansion culture device according to claim 1, characterized in that: The control mechanism (4) includes an output pipe (401) which is connected to the top of the outer wall of the culture tank (1). A fixing frame (402) is fixedly connected around the outer wall of the output pipe (401), and a flow limiting component (403) is fixedly connected to the rear side of the outer wall of the fixing frame (402).

6. The photosynthetic bacteria expansion culture device according to claim 5, characterized in that: The flow limiting component (403) includes a proportional valve (4031), which is fixedly connected to the rear side of the outer wall of the fixing frame (402), and a valve (4032) is installed on the front side of the outer wall of the proportional valve (4031).

7. The photosynthetic bacteria expansion culture device according to claim 3, characterized in that: A support frame (5) is fixedly connected to the top side of the feed pipe (301), and a screw (6) is threadedly connected to the outer wall side of the support frame (5).

8. The photosynthetic bacteria expansion culture device according to claim 1, characterized in that: An observation window (7) is fixedly connected to one side of the outer wall of the culture tank (1), and an exhaust hole (8) is provided at the top of the outer wall of the culture tank (1).

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